Integrated kit for detecting african swine fever virus

Abstract

The application relates to the technical field of virus detection, in particular to an integrated kit for African swine fever virus detection, which comprises a box body bottom capsule plate and a box body upper cover, the box body bottom capsule plate and the box body upper cover can be covered to form a box body structure, the box body bottom capsule plate is a plate-shaped structure with an internal cavity and has elasticity, a virus detection card is arranged on the box body bottom capsule plate, and a sample adding window is arranged on the virus detection card; the integrated kit integrates the box body and a sample suction assembly, not only enhances the protection effect on the virus detection card, but also is more convenient for detection, the sample adding window does not need to be exposed, a rubber head dropper and other sample liquid transfer tools do not need to be used to directly draw sample liquid, the utilization rate of the box body is improved, and the probability of cross contamination is reduced.

Description

Technical Field

[0001] This invention relates to the field of virus detection technology, specifically to an integrated reagent kit for detecting African swine fever virus. Background Technology

[0002] African swine fever virus (ASFV) is highly contagious and extremely pathogenic. Acute cases are characterized by high fever, short course of illness, high mortality, widespread internal organ hemorrhage, and respiratory and nervous system dysfunction. Pigs of all ages are susceptible, with a short incubation period and a morbidity and mortality rate as high as 100%. Currently, there is no effective treatment for ASFV, causing enormous economic losses to the global pig farming industry. Currently, the most common method for pig farmers to detect ASFV is through ASFV test kits. Existing technology involves packaging these test kits within a reagent kit. Paper boxes offer poor protection, while plastic boxes, although providing sufficient support, are wasteful. Traditional plastic boxes only serve a protective function and do not participate in the testing process. Furthermore, using the test kit exposes the sample application window and requires sample transfer tools, posing a high risk of cross-contamination. Summary of the Invention

[0003] The purpose of this invention is to provide an integrated reagent kit for detecting African swine fever virus, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: an integrated reagent kit for detecting African swine fever virus, comprising a bottom capsule and a top cover, wherein the bottom capsule and the top cover can be closed to form a box structure. The bottom capsule is a plate-like structure with an internal cavity and is elastic. A virus detection card is disposed on the bottom capsule, and the virus detection card has a sample application window for adding sample solution. A sample aspiration tube is disposed outside the virus detection card, and the sample aspiration tube is a tubular structure with an internal cavity. A rubber connecting tube is disposed between the sample aspiration tube and the bottom capsule. A sealing and fixed adhesive rotating seat is attached to the test card, which covers the sample application window. An inner tube is rotatably mounted inside the adhesive rotating seat, and the upper end of the inner tube is fixedly connected to the sample aspiration tube. The adhesive rotating seat and the inner tube are in sealed contact. A limiting upper ring is fixedly mounted on the inner wall surface of the adhesive rotating seat, and a limiting lower ring is fixedly mounted on the outer surface of the inner tube. The limiting upper ring is located above the limiting lower ring and limits the limiting lower ring. A locking structure is provided between the bottom bladder plate and the top cover of the box. When the bottom bladder plate and the top cover of the box are closed, the locking structure will interrupt the connection of the rubber connecting tube.

[0005] The locking structure includes a top cover shaft and a control frame. The top cover shaft is fixedly installed on the upper cover of the box, and the control frame is fixedly installed on the bottom plate of the box. The rubber connecting tube passes through the control frame.

[0006] The inner wall surface of the control frame is fixedly provided with a mating pressure ridge, and a movable pressure block is provided in the control frame. The rubber connecting pipe passes between the mating pressure ridge and the movable pressure block.

[0007] An elastic connecting plate is fixedly installed on the lower surface of the movable pressure block. The elastic connecting plate is elastic, and the lower end of the elastic connecting plate is fixedly installed on the upper surface of the bottom plate of the box.

[0008] The movable pressure block is provided with a guide slope. When the bottom plate of the box and the top cover of the box are closed together, the top shaft of the cover will press against the guide slope, pushing the movable pressure block to move towards the mating pressure edge, and clamping and sealing the rubber connecting tube.

[0009] The inner wall surface of the sample aspiration tube is provided with a positioning blind hole, and a central disk is provided inside the sample aspiration tube. The positioning blind hole is coaxially corresponding to the central disk.

[0010] Blind hole shafts are symmetrically arranged on the upper and lower surfaces of the central disk. The blind hole shafts are rotatably inserted into the positioning blind holes. Arc-curved blades are fixedly arranged on the side surface of the central disk. The arc-curved blades are curved in an arc shape and are evenly distributed in a circular array around the central disk.

[0011] The bottom plate of the box has a cover rim, and the top cover of the box is sealed to the cover rim. A fixing clip is fixedly installed on the upper surface of the bottom plate of the box, and the virus detection card is held in the fixing clip.

[0012] The sample aspiration tube is connected to an aspiration and gathering part at its end. A protective bracket is fixedly installed on the bottom plate of the box. A positioning groove is provided on the protective bracket, and the aspiration and gathering part is placed in the positioning groove.

[0013] The inner wall surface of the bottom plate of the box is fixedly provided with a reinforcing inner wall ridge, and the virus detection card is provided with a result observation window.

[0014] Compared with the prior art, the beneficial effects of the present invention are:

[0015] This invention presents an integrated reagent kit that combines the kit body and sample aspiration component into one unit. This design not only enhances the protection of the virus detection card but also facilitates testing. It eliminates the need for an exposed sample application window and the use of sample transfer tools such as droppers, allowing for direct sample aspiration and improving kit utilization while reducing the probability of cross-contamination. Furthermore, during routine use, the virus detection card can still be removed by peeling off the adhesive transposable from the card for regular application.

[0016] By using a locking structure in conjunction with the rubber connecting tube and the bottom bladder plate of the box, the rubber connecting tube can be automatically shut off after the top cover and the bottom bladder plate of the box are closed. This allows the gas in the bottom bladder plate to be sealed inside the bottom bladder plate, making the bottom bladder plate less prone to deformation under pressure and providing better protection.

[0017] By combining the designed sample aspiration tube, central disc, and curved blades, the sample liquid flowing inside the sample aspiration tube is stirred and mixed again when it is drawn through the tube, improving the uniformity of the sample liquid and making the test results more accurate. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the present invention when it is fully closed.

[0019] Figure 2 This is an open schematic diagram of the overall structure of the present invention.

[0020] Figure 3 This is a schematic diagram showing the overall structure of the present invention from another angle.

[0021] Figure 4 This is a three-dimensional half-section diagram of the adhesive rotating base of the present invention.

[0022] Figure 5 for Figure 4 Enlarged schematic diagram of region A in the middle.

[0023] Figure 6 This is a three-dimensional half-section schematic diagram of the control box of the present invention.

[0024] Figure 7 for Figure 6 Enlarged schematic diagram of region B in the middle.

[0025] Figure 8 This is a horizontal half-section diagram of the sample aspiration tube of the present invention.

[0026] Figure 9 This is an exploded view of part of the structure of the present invention.

[0027] Figure 10 for Figure 9 Enlarged schematic diagram of region C in the middle.

[0028] In the diagram: 1. Box bottom plate; 2. Box top cover; 3. Virus detection card; 4. Sample application window; 5. Sample aspiration tube; 6. Rubber connecting tube; 7. Adhesive turntable; 8. Turntable inner tube; 9. Upper limiting ring; 10. Lower limiting ring; 201. Top cover shaft; 202. Control frame; 203. Fitting pressure edge; 204. Movable pressure block; 205. Elastic connecting plate; 206. Guide slope; 501. Positioning blind hole; 502. Central disc; 503. Blind hole shaft; 504. Curved blade; 505. Suction gathering part; 101. Capsule cover; 102. Fixing clip; 103. Protective bracket; 104. Positioning groove; 105. Reinforced inner wall edge; 301. Result observation window. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] This invention provides a technical solution: an integrated reagent kit for detecting African swine fever virus, such as... Figure 2 As shown, the box includes a bottom liner 1 and a top cover 2. The bottom liner 1 and the top cover 2 can be closed to form a box structure. The bottom liner 1 is a plate-like structure with an internal cavity and is elastic. A virus detection card 3 is installed on the bottom liner 1. The virus detection card 3 is an African swine fever virus antigen colloidal gold detection card. Figure 5 As shown, the virus test card 3 has a sample application window 4 for adding the sample solution. A sample aspiration tube 5 is located on the outside of the virus test card 3. The sample aspiration tube 5 is a tubular structure with an internal cavity. A rubber connecting tube 6 connects the sample aspiration tube 5 and the bottom plate 1 of the test box. An adhesive rotating seat 7 is sealed and fixed to the virus test card 3. The virus test card 3 and the adhesive rotating seat 7 are fixed together with adhesive and can be torn apart. The adhesive rotating seat 7 covers the sample application window 4, and the interior of the adhesive rotating seat 7 rotates. A rotating inner tube 8 is provided, and the upper end of the rotating inner tube 8 is fixedly connected to the sample aspiration tube 5. The adhesive rotating seat 7 is in sealed contact with the rotating inner tube 8. A limiting upper ring 9 is fixedly provided on the inner wall surface of the adhesive rotating seat 7, and a limiting lower ring 10 is fixedly provided on the outer surface of the rotating inner tube 8. The limiting upper ring 9 is located above the limiting lower ring 10 and limits the limiting lower ring 10. A locking structure is provided between the bottom bladder plate 1 and the top cover 2 of the box. When the bottom bladder plate 1 and the top cover 2 of the box are closed, the locking structure will interrupt the connection of the rubber connecting tube 6.

[0031] The locking structure includes a top cover shaft 201 and a control frame 202. The top cover shaft 201 is fixedly installed on the upper cover 2 of the box body, and the control frame 202 is fixedly installed on the bottom liner 1 of the box body. A rubber connecting tube 6 passes through the control frame 202. A mating pressure ridge 203 is fixedly provided on the inner wall surface of the control frame 202. A movable pressure block 204 is provided in the control frame 202, and the rubber connecting tube 6 passes between the mating pressure ridge 203 and the movable pressure block 204. An elastic connecting plate 205 is fixedly provided on the lower surface of the movable pressure block 204. The elastic connecting plate 205 is elastic, and its lower end is fixedly installed on the upper surface of the bottom liner 1 of the box body. A guide slope 206 is provided on the movable pressure block 204.

[0032] like Figure 7 As shown, when the bottom bladder 1 and the top cover 2 of the box are closed together, the top cover shaft 201 will press against the guide slope 206, pushing the movable pressure block 204 to move towards the mating pressure ridge 203, clamping and sealing the rubber connecting tube 6; after the rubber connecting tube 6 is clamped and sealed, the gas in the bottom bladder 1 of the box will be locked inside the bottom bladder 1 of the box, which greatly improves the pressure resistance of the bottom bladder 1 of the box. When the bottom bladder 1 of the box is squeezed, the air pressure will support the bottom bladder 1 of the box, reduce the deformation of the bottom bladder 1 of the box, and improve the protective effect of the bottom bladder 1 of the box.

[0033] The inner wall surface of the sample aspiration tube 5 is provided with a positioning blind hole 501, and a central disk 502 is provided inside the sample aspiration tube 5. The positioning blind hole 501 and the central disk 502 are coaxially corresponding.

[0034] Blind hole shafts 503 are symmetrically arranged on the upper and lower surfaces of the central disk 502. The blind hole shafts 503 are rotatably inserted into the positioning blind holes 501. Curved blades 504 are fixedly arranged on the side surface of the central disk 502. The curved blades 504 are curved in an arc shape and are evenly distributed in a circular array around the central disk 502. Figure 8 As shown, the structure formed by the central disc 502 and the curved blade 504 can be set in multiple sets inside the sample aspiration tube 5, thereby enhancing the stirring effect.

[0035] The bottom plate 1 of the box body has a cover 101. The top cover 2 of the box body is sealed to the cover 101. A fixing clip 102 is fixedly installed on the upper surface of the bottom plate 1 of the box body. The virus detection card 3 is clamped in the fixing clip 102. The fixing clip 102 limits and clamps the virus detection card 3, and the two can be separated.

[0036] The end of the sample aspiration tube 5 is connected to an aspiration gathering part 505. The aspiration gathering part 505 makes it easier for the sample aspiration tube 5 to draw sample liquid. A protective bracket 103 is fixedly installed on the bottom plate 1 of the box. A positioning groove 104 is opened on the protective bracket 103. The aspiration gathering part 505 is placed in the positioning groove 104. The protective bracket 103 supports the aspiration gathering part 505, so that the sample aspiration tube 5 is prevented from deforming and collapsing.

[0037] The inner wall surface of the bottom plate 1 of the box is fixedly provided with a reinforcing inner wall ridge 105. By providing the reinforcing inner wall ridge 105, the bottom plate 1 of the box has stronger support and sufficient suction force for elastic reset after the bottom plate 1 of the box is pressed. The virus detection card 3 is provided with a result observation window 301.

[0038] The integrated reagent kit of this invention, when used, requires separating the top cover 2 of the box from the bottom plate 1 of the box, as follows: Figure 3 and Figure 7 As shown, the top cover shaft 201 will be pulled out from the control box 202, causing the movable pressure block 204 to reset and no longer squeeze the rubber connecting tube 6. At this time, the rubber connecting tube 6 is in the connected state.

[0039] like Figure 6 As shown, rotate the sample aspiration tube 5 180° around the adhesive turntable 7, press the bottom capsule 1 of the box to expel some of the gas from the bottom capsule 1, then insert the aspiration convergence part 505 into the sample solution, keeping the virus detection card 3 facing upwards, and release the bottom capsule 1. At this time, the bottom capsule 1 elastically returns to its original position and aspirates gas. The sample solution is drawn into the sample aspiration tube 5 through the aspiration convergence part 505 and flows towards the rubber connecting tube 6; as... Figure 5 As shown, when the sample liquid flows over the inner tube 8 of the transducer, it will fall into the inner tube 8 of the transducer under the action of gravity, and thus enter the sample application window 4.

[0040] Once the sample solution enters the sample application window 4, the virus detection card 3 will test the sample solution and display the results through the result observation window 301. When both C and T show horizontal bars, it indicates that there is African swine fever virus infection, and the result is positive.

[0041] In the above process, such as Figure 8 As shown, when the sample liquid flows in the sample aspiration tube 5, the curved blade 504, being curved plate-shaped, generates a thrust difference, causing the central disk 502 to move along the edge. Figure 8 Rotating clockwise from a perspective in the sample tube 5 mixes and stirs the sample solution flowing in the sample aspiration tube 5, improving the uniformity of the sample solution.

[0042] In normal use, the virus detection card 3 can be directly removed from the fixing clip 102, and then the adhesive adapter 7 can be separated from the virus detection card 3. The adhesive adapter 7 is attached to the virus detection card 3 with adhesive. It can be manually separated to remove the virus detection card 3 for normal operation.

[0043] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An integrated kit for detecting African swine fever virus, comprising a box body bottom capsule plate (1) and a box body upper cover (2), the box body bottom capsule plate (1) and the box body upper cover (2) can be covered to form a box body structure, characterized in that: The box body bottom capsule plate (1) is a plate-shaped structure with an internal cavity and has elasticity, a virus detection card (3) is arranged on the box body bottom capsule plate (1), a sample adding window (4) is formed in the virus detection card (3), the sample adding window (4) is used for adding a sample liquid, a sample suction tube (5) is arranged outside the virus detection card (3), the sample suction tube (5) is a tubular structure with an internal cavity, a rubber connecting tube (6) is arranged in communication between the sample suction tube (5) and the box body bottom capsule plate (1), a paste transfer seat (7) is fixedly attached to the virus detection card (3), the paste transfer seat (7) covers the sample adding window (4), a transfer seat inner tube (8) is rotatably arranged in the paste transfer seat (7), the upper end of the transfer seat inner tube (8) is fixedly connected with the sample suction tube (5), the paste transfer seat (7) is in sealing contact with the transfer seat inner tube (8), a limiting upper ring (9) is fixedly arranged on the inner wall surface of the paste transfer seat (7), a limiting lower ring (10) is fixedly arranged on the outer surface of the transfer seat inner tube (8), the limiting upper ring (9) is located above the limiting lower ring (10) and limits the limiting lower ring (10), a lock catch structure is arranged between the box body bottom capsule plate (1) and the box body upper cover (2), when the box body bottom capsule plate (1) and the box body upper cover (2) are overlapped with each other, the lock catch structure interrupts the communication of the rubber connecting tube (6).

2. The integrated kit for detecting African swine fever virus according to claim 1, characterized by: The lock catch structure comprises a cover top shaft (201) and a control square box (202), the cover top shaft (201) is fixedly installed on the box body upper cover (2), the control square box (202) is fixedly installed on the box body bottom capsule plate (1), and the rubber connecting tube (6) penetrates through the control square box (202).

3. The integrated kit for detecting African swine fever virus according to claim 2, characterized in that: A matching pressing rib (203) is fixedly arranged on the inner wall surface of the control square box (202), and a movable pressing block (204) is arranged in the control square box (202), and the rubber connecting tube (6) penetrates between the matching pressing rib (203) and the movable pressing block (204).

4. The integrated kit for detecting African swine fever virus according to claim 3, characterized in that: An elastic connecting plate (205) is fixedly arranged on the lower surface of the movable pressing block (204), the elastic connecting plate (205) has elasticity, and the lower end of the elastic connecting plate (205) is fixedly installed on the upper surface of the box body bottom capsule plate (1).

5. The integrated kit for detecting African swine fever virus according to claim 4, characterized in that: A guide inclined surface (206) is formed in the movable pressing block (204), when the box body bottom capsule plate (1) and the box body upper cover (2) are overlapped with each other, the cover top shaft (201) is located on the guide inclined surface (206) and pushes the movable pressing block (204) to move towards the matching pressing rib (203), so that the rubber connecting tube (6) is clamped and closed.

6. The integrated kit for detecting African swine fever virus according to claim 1, characterized in that: A positioning blind hole (501) is formed in the inner wall surface of the sample suction tube (5), and a center disc body (502) is arranged in the sample suction tube (5), and the positioning blind hole (501) is coaxially corresponding to the center disc body (502).

7. The integrated kit for detecting African swine fever virus according to claim 6, characterized in that: The upper and lower surfaces of the center disc body (502) are symmetrically provided with blind hole rotating shafts (503), the blind hole rotating shafts (503) are rotatably arranged in the positioning blind holes (501), the side surface of the center disc body (502) is fixedly provided with arc bending blades (504), the arc bending blades (504) are arc bending, and are uniformly distributed in a circumferential array type with the center disc body (502) as the center.

8. The integrated kit for detecting African swine fever virus according to claim 1, characterized in that: The box body bottom capsule plate (1) is provided with a capsule plate cover eave (101), the box body upper cover (2) is in sealing cooperation with the capsule plate cover eave (101), the upper surface of the box body bottom capsule plate (1) is fixedly provided with a fixed clamp (102), and the virus detection card (3) is clamped in the fixed clamp (102).

9. The integrated kit for detecting African swine fever virus according to claim 1, characterized in that: The end of the sample suction pipe (5) is communicatively provided with a suction gathering part (505), the box body bottom capsule plate (1) is fixedly provided with a protection bracket (103), the protection bracket (103) is provided with a positioning sink (104), and the suction gathering part (505) is placed in the positioning sink (104).

10. The integrated kit for detecting African swine fever virus according to claim 1, characterized in that: The inner wall surface of the box body bottom capsule plate (1) is fixedly provided with a reinforced inner wall rib (105), and the virus detection card (3) is provided with a result observation window (301).

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