A traditional Chinese medicine composition for preventing and treating infectious anemia in chickens and its preparation method

Abstract

This invention belongs to the field of veterinary drug technology and relates to a traditional Chinese medicine composition for preventing and treating infectious anemia in chickens and its preparation method. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20-40 parts of *Smilax china*, 10-30 parts of *Smilax glabra* root, 10-30 parts of hematite, 10-20 parts of *Polygonum multiflorum*, and 10-20 parts of *Hemiberlesia asiatica*. This traditional Chinese medicine composition can rapidly improve the mental state, feed intake, and anemia status of sick chickens, reduce viral load, reduce mortality, and is green and safe. It can meet the needs of actual production (especially for laying hens) for the prevention and control of infectious anemia in chickens, reduce infection losses, and improve breeding efficiency.

Description

Technical Field

[0001] This invention belongs to the field of veterinary medicine technology, specifically relating to a traditional Chinese medicine composition for preventing and treating infectious anemia in chickens and its preparation method. Background Technology

[0002] Chicken infectious anemia (CAV) is an immunosuppressive and egg-borne infectious disease primarily affecting chicks, caused by chicken infectious anemia virus (CAV). It is characterized by aplastic anemia and systemic lymphoid atrophy. Chickens of all ages and breeds can be infected, with a mortality rate generally between 5% and 60%, affecting poultry growth and egg production. This disease can occur year-round and is one of the major diseases affecting the poultry industry.

[0003] The typical clinical symptom of avian infectious anemia virus (CAV) is anemia. Seven days after infection, 1-day-old SPF chickens, compared to healthy chickens, show decreased appetite, smaller weight gain, smaller body size, and a slightly paler comb, eyelids, and visible mucous membranes. Fourteen days after infection, ruffled feathers, drooping wings, lethargy, and skin lesions with serous exudate are observed. Dissection of affected chickens reveals hematocrit (HCT) values ​​<27%. Observation of immune organs shows the most characteristic pathological changes: yellowing and atrophy of the bone marrow in hematopoietic organs, and atrophy and damage to lymphoid organs and cells. After CAV infection, proerythroblasts and hematopoietic cells in the bone marrow become enlarged and deformed, the number of mature red blood cells decreases significantly, adipose tissue gradually replaces the atrophied hematopoietic tissue, and the bone marrow color changes from normal red to yellowish-white. After chickens are infected with CAV, the medullary cells in the thymus gradually die, the cortical cells gradually lyse, and as the number of lymphocytes continues to decrease, the thymus tissue atrophies, and the thymus organ index is also lower than normal. The volume of lymphatic vesicles in the bursa of Fabricius gradually decreases, and the number of lymphocytes drops sharply. The spleen loses its color, and some cells in the lymph nodes degenerate, and in severe cases, infiltration and necrosis may occur. The liver will also become swollen, soft in texture, disordered hepatic cords, and necrosis may occur in the central part of the liver lobules.

[0004] Currently, in terms of drug prevention and treatment, veterinarians usually use antibiotics and antibacterial drugs to control infection, while also giving chickens nutritional supplements to improve their immunity and restore their hematopoietic ability. However, antibiotics have no antiviral effect and are mainly used clinically to control secondary bacterial infections. Nutritional supplements can supplement iron and blood to play a supporting role in symptomatic treatment. There is a lack of effective antiviral drugs for infectious anemia in chickens on the market. Especially with the ban on antibiotics in laying hens, green and antibiotic-free drugs for treating infectious anemia in chickens have become one of the new issues that researchers in this field urgently need to solve. Summary of the Invention

[0005] In view of the problems existing in the prior art, the purpose of this invention is to provide a traditional Chinese medicine composition for preventing and treating infectious anemia in chickens and its preparation method.

[0006] To achieve the objectives of this invention, the technical solution adopted is as follows:

[0007] The first aspect of the present invention provides a traditional Chinese medicine composition for preventing and treating infectious anemia in chickens. The traditional Chinese medicine composition is mainly prepared from the following raw materials in parts by weight: 20-40 parts of black vine, 10-30 parts of yellow root, 10-30 parts of hematite, 10-20 parts of fern root, and 10-20 parts of red baby's breath.

[0008] Preferably, the traditional Chinese medicine composition is mainly prepared from the following raw materials in parts by weight: 30 parts of black vine, 20 parts of yellow root, 20 parts of hematite, 15 parts of fern root, and 15 parts of red baby's foot.

[0009] Preferably, the dosage form of the traditional Chinese medicine composition is a powder or an oral liquid.

[0010] Preferably, the dosage form of the traditional Chinese medicine composition is an oral liquid, and the traditional Chinese medicine composition further includes sodium benzoate and niparatinin.

[0011] Preferably, the amount of sodium benzoate used is 0.1% to 0.2% of the total weight of the active pharmaceutical ingredient; and the amount of paraben used is 0.05% to 0.1% of the total weight of the active pharmaceutical ingredient.

[0012] The second aspect of this invention provides a method for preparing a traditional Chinese medicine composition for preventing and treating infectious anemia in chickens, wherein raw materials such as black vine, yellow root, hematite, fern root, and red baby's ear are mixed, pulverized, and prepared into corresponding dosage forms according to pharmaceutical requirements.

[0013] When the dosage form of the traditional Chinese medicine composition is a powder, the specific process of its preparation is as follows: mix, pulverize, and sieve the black vine, yellow root, hematite, fern root, and red baby's ear until the particle size reaches 60-200 mesh, and then package them to obtain the finished product.

[0014] Preferably, the optimal clinical dosage for preventing and treating infectious anemia in chickens is: 1 kg of product mixed with 200 kg of feed, used continuously for 3-5 days.

[0015] When the dosage form of the traditional Chinese medicine composition is an oral liquid, it is prepared using a steam explosion crushing extraction-pressure filtration-concentration preparation technology.

[0016] Preferably, the steps are as follows:

[0017] (1) Crush black vine, yellow root, hematite, fern root, and red baby into coarse powder and load them into the steam explosion device. Introduce saturated steam, then open the pressure relief valve to carry out steam explosion crushing.

[0018] (2) Take the fine powder obtained in step (1) and add 8 to 10 times the volume of water for the first extraction and decoction, and add 6 to 7 times the volume of water for the second extraction and decoction.

[0019] (3) Transfer the medicinal liquid and residue from step (2) into a hydraulic pressure filter for filtration and separation;

[0020] (4) Transfer the separated liquid from step (3) into a concentrator and concentrate it until the weight of the liquid is 1.0 ± 0.1 times the total weight of the Chinese herbal raw materials. Add 0.1% to 0.2% sodium benzoate and 0.05% to 0.1% paraben to the concentrated liquid as preservatives to obtain the oral liquid.

[0021] Preferably, the steam pressure in step (1) is 4 to 6 MPa; the pressure is maintained for 5 to 8 minutes.

[0022] Preferably, in step (2), the initial decocting temperature is 85-90℃ and the decocting time is 30-60 min; the secondary decocting temperature is 60-65℃ and the decocting time is 10-30 min.

[0023] Preferably, the filter cloth pore size in step (3) is 1000-1200 mesh.

[0024] Preferably, in step (4), an MVR concentrator is used, and the concentration temperature is 55-60℃.

[0025] Preferably, the optimal clinical dosage for the prevention and treatment of infectious anemia in chickens is: 250ml of product diluted in 200kg of water, used continuously for 3-5 days.

[0026] All components in the traditional Chinese medicine composition of this invention can be purchased commercially available products.

[0027] Black Blood Vine: The old stem of *Mucuna macrcocar pa Wall.Cat.*, a legume. It can be harvested year-round; the stems and vines are cut and used fresh, or sliced ​​and dried. It is bitter, astringent, and cool in nature. It enters the liver and kidney meridians, nourishing and invigorating blood; clearing the lungs and moistening dryness; and promoting blood circulation. It is used to treat anemia; irregular menstruation; dry cough due to lung heat; hemoptysis; lower back and knee pain; rheumatic pain; numbness in the hands and feet; and paralysis.

[0028] Yellow root: The root of *Prismatomeris tetrandra* (Roxb.) k. Schum, a plant in the Rubiaceae family. It can be harvested year-round. Wash, slice, and sun-dry. It is slightly bitter and has cooling properties. It cools the blood and stops bleeding; promotes diuresis and reduces jaundice; disperses blood stasis and strengthens muscles. It is mainly used for bleeding gums; anemia; hepatitis; rheumatoid arthritis; traumatic injuries; and urinary tract infections.

[0029] Haematitum: a corundum group mineral, mainly containing ferric oxide (Fe2O3). It has a wide range of clinical applications and is good at calming the liver and suppressing yang, sedating and suppressing adverse reactions, cooling the blood and stopping bleeding. It is mainly used to treat headache, dizziness, palpitations, anemia, vomiting, hiccups, esophageal obstruction, cough, asthma, hematochezia, hematuria, etc.

[0030] Potentilla anserina L., a plant in the Rosaceae family, is a tuberous root. Harvested from June to September, impurities are removed, it is washed, and then sun-dried. It is sweet, bitter, and cold in nature. It enters the spleen and stomach meridians. It tonifies qi and blood, strengthens the spleen and stomach, and promotes the production of body fluids and quenches thirst. It is mainly used for spleen deficiency diarrhea, post-illness anemia, malnutrition, edema, and rheumatic pain.

[0031] Red Boy: The dried tuber of *Dioscorea cirrhosa*, Lour., a plant in the Dioscoreaceae family. Harvested in summer and autumn, washed, sliced, and sun-dried. Slightly bitter and astringent, slightly cold in nature. Used to stop bleeding, cool the blood, and replenish blood. Used for metrorrhagia, postpartum hemorrhage, hemoptysis, hematuria, upper gastrointestinal bleeding, and anemia.

[0032] Compared with the prior art, the positive and beneficial effects achieved by the present invention are as follows:

[0033] The formulation mechanism of the traditional Chinese medicine composition of this invention is as follows: Black vine is bitter and cool in nature, clears heat and moistens dryness, promotes blood circulation and removes blood stasis, and is the principal drug; Yellow root is bitter and cool in nature, cools blood and disperses blood stasis; Hematite is bitter and cold in nature, calms the liver and cools blood, and together they are the assistant drugs, assisting the principal drug in clearing blood stasis and removing blood stasis; Fern root is neutral in nature and sweet in taste, invigorates qi, nourishes blood and strengthens the spleen; Red baby's foot is astringent and slightly cold in taste, invigorates blood and nourishes blood, and is the adjuvant drug, strengthening the spleen and nourishing blood; When all the drugs are used together, they work together to clear heat and remove blood stasis, and nourish blood and replenish deficiency.

[0034] The traditional Chinese medicine composition provided in this application can rapidly improve the chicken's spirit, feed intake, and anemia, reduce viral load, reduce mortality, and is green and safe. It can basically meet the needs of actual production (especially for laying hens) in the prevention and control of infectious anemia in chickens, reduce infection losses, and improve breeding efficiency. Detailed Implementation

[0035] The present invention will be further described in detail below through specific embodiments, but this does not limit the scope of the present invention.

[0036] Experiment 1

[0037] Example 1

[0038] A traditional Chinese medicine composition for preventing and treating infectious anemia in chickens, in the form of a powder, is prepared from the following raw materials by weight: 300g of black vine, 200g of yellow root, 200g of hematite, 150g of fern root, and 150g of red baicalensis.

[0039] The preparation method is as follows: weigh out the black vine, yellow root, hematite, fern root, and red baby's ear according to weight, mix them, crush and sieve them to achieve a particle size of 60 mesh, and then package them to obtain the Chinese herbal composition.

[0040] Example 2

[0041] A traditional Chinese medicine composition for preventing and treating infectious anemia in chickens, in the form of a powder, is prepared from the following raw materials by weight: 200g of black vine, 150g of yellow root, 150g of hematite, 100g of fern root, and 100g of red baicalensis.

[0042] The preparation method is as follows: weigh out the black vine, yellow root, hematite, fern root, and red baby's ear according to weight, mix them, crush and sieve them to achieve a particle size of 120 mesh, and then package them to obtain the Chinese herbal composition.

[0043] Example 3

[0044] A traditional Chinese medicine composition for preventing and treating infectious anemia in chickens, in the form of a powder, is prepared from the following raw materials by weight: 400g of black vine, 100g of yellow root, 300g of hematite, 200g of fern root, and 200g of red baicalensis.

[0045] The preparation method is as follows: weigh out the black vine, yellow root, hematite, fern root, and red baby's ear according to weight, mix them, crush and sieve them to achieve a particle size of 200 mesh, and then package them to obtain the Chinese herbal composition.

[0046] Example 4

[0047] A traditional Chinese medicine composition for the prevention and treatment of infectious anemia in chickens, in the form of an oral liquid, is prepared from the following raw materials by weight: 300g of black vine, 200g of yellow root, 200g of hematite, 150g of fern root, and 150g of red baicalensis; with 0.1% sodium benzoate and 0.1% parabens as preservatives by volume.

[0048] The preparation method is as follows:

[0049] (1) Crush black vine, yellow root, hematite, fern root, and red baby into coarse powder and put them into the steam explosion device. Introduce saturated steam at a pressure of 3.5 MPa. Maintain the pressure for 4 minutes. Then open the pressure relief valve to perform steam explosion crushing.

[0050] (2) Take the medicinal materials after steam explosion in step (1) and add 8 times the volume of water for the first extraction and decoction. The decoction temperature is 90℃ and the decoction time is 30min. Add 6 times the volume of water for the second extraction and decoction. The decoction temperature is 65℃ and the decoction time is 10min. After extraction, the medicinal liquid and the residue are transferred to a hydraulic high-efficiency filter press. The filter cloth has a pore size of 1100 mesh. After filter press, the medicinal liquid is transferred to an MVR concentrator for concentration. The concentration temperature is 55℃. The concentration is reduced and concentrated until the weight of the medicinal liquid is 1.0±0.1 times the total weight of the Chinese herbal raw materials. Add 0.1% sodium benzoate and 0.1% paraben as preservatives to the concentrated medicinal liquid to obtain the oral liquid.

[0051] Example 5

[0052] A traditional Chinese medicine composition for the prevention and treatment of infectious anemia in chickens, in the form of an oral liquid, is prepared from the following raw materials by weight: 280g of black vine, 200g of yellow root, 180g of hematite, 150g of fern root, and 120g of red baicalensis; with 0.1% sodium benzoate and 0.1% parabens as preservatives by volume.

[0053] The preparation method is as follows:

[0054] (1) Crush black vine, yellow root, hematite, fern root, and red baby into coarse powder and put them into the steam explosion device. Introduce saturated steam at a pressure of 3.5 MPa. Maintain the pressure for 4 minutes. Then open the pressure relief valve to perform steam explosion crushing.

[0055] (2) Take the medicinal materials after steam explosion in step (1) and add 8 times the volume of water for the first extraction and decoction. The decoction temperature is 90℃ and the decoction time is 30min. Add 6 times the volume of water for the second extraction and decoction. The decoction temperature is 65℃ and the decoction time is 10min. After extraction, the medicinal liquid and the residue are transferred to a hydraulic high-efficiency filter press. The filter cloth has a pore size of 1100 mesh. After filter press, the medicinal liquid is transferred to an MVR concentrator for concentration. The concentration temperature is 55℃. The concentration is reduced and concentrated until the weight of the medicinal liquid is 1.0±0.1 times the total weight of the Chinese herbal raw materials. Add 0.1% sodium benzoate and 0.1% paraben as preservatives to the concentrated medicinal liquid to obtain the oral liquid.

[0056] Example 6

[0057] A traditional Chinese medicine composition for preventing and treating infectious anemia in chickens, in the form of an oral liquid, is prepared from the following raw materials by weight: 400g of black vine, 300g of yellow root, 100g of hematite, 150g of fern root, and 200g of red baicalensis; with 0.2% sodium benzoate and 0.05% parabens as preservatives.

[0058] The preparation method is as follows:

[0059] (1) Crush black vine, yellow root, hematite, fern root, and red baby into coarse powder and load them into the steam explosion device. Introduce saturated steam at a pressure of 3.5 MPa. Maintain the pressure for 5 minutes. Then open the pressure relief valve to perform steam explosion crushing.

[0060] (2) Take the medicinal materials after steam explosion in step (1) and add 8 times the volume of water for the first extraction and decoction. The decoction temperature is 85℃ and the decoction time is 60min. Add 6 times the volume of water for the second extraction and decoction. The decoction temperature is 60℃ and the decoction time is 30min. After extraction, the medicinal liquid and the residue are transferred to a hydraulic high-efficiency filter press. The filter cloth pore size is 1000 mesh. After filter press, the medicinal liquid is transferred to an MVR concentrator for concentration. The concentration temperature is 57℃. The concentration is reduced and concentrated until the weight of the medicinal liquid is 1.0±0.1 times the total weight of the Chinese herbal raw materials. Add 0.2% sodium benzoate and 0.05% paraben as preservatives to the concentrated medicinal liquid to obtain the oral liquid.

[0061] Effect verification

[0062] 1. Materials and Methods

[0063] 1.1 The CAV standard strain AV1550 was purchased from the National Veterinary Microbiology Culture Collection Center, with a virus content of 105.3 EID50 / 0.2 mL.

[0064] 1.2 180 one-day-old SPF chickens were purchased from the Experimental Animal Center of Zhengzhou University.

[0065] 1.3 Grouping: One-day-old SPF chickens were randomly divided into 6 groups of 30 birds each, based on their body weight. Each group was housed independently in a separate isolation enclosure. Feed and purified water were sterilized and disinfected. The chickens were raised for 21 consecutive days. Except for the control group, all other groups of one-day-old SPF chickens were infected via intramuscular injection into the pectoral cavity. Based on preliminary experimental results, the infection dose per chicken was set at 10,000 EID. 50 / 0.1mL. Chicks were isolated and observed for 21 days after infection. The body weight of each chicken in each group was measured on the day of infection, and the body weight, necropsy, and thymocyte viral load were measured 21 days after infection. Specific groupings are shown in Table 1 below.

[0066] Table 1 SPF Chicken Grouping Table

[0067]

[0068] Note: The Angelica Blood-Nourishing Decoction group was prepared according to the method in "Traditional Chinese Medicine Formulae", with a specification of 1g crude drug / ml; Qingwen Baidu Powder was purchased from Shandong Dayu Animal Pharmaceutical Co., Ltd. (Veterinary Drug Approval No. 151715165, 1000g / bag, production batch number 23060712); Groups 1 and 4 of the invention were prepared according to the above preparation method.

[0069] 1.4 Clinical Symptom Observation and Weight Monitoring: Chickens were observed for 21 days post-infection. All chickens were weighed on the day of infection and at the end of the experiment to compare weight gain. Clinical symptoms were observed daily during the observation period.

[0070] 1.5 Hematocrit test: Blood samples were collected 21 days post-infection for hematocrit testing.

[0071] 1.6 Necropsy and Pathological Examination: During the observation period, dead chickens were necropsed at any time. At the end of the experiment, all surviving chickens were necropsed, and gross pathological changes in the thymus, bursa of Fabricius, etc., were observed. Viral load was measured in selected thymus samples. The results are shown in Table 2 below.

[0072] Table 2 Clinical symptoms and weight of each group

[0073]

[0074] Note: Different letters (a, b) indicate significant differences (P < 0.05).

[0075] 2. Results Analysis

[0076] 2.1 Clinical Symptoms and Weight: One-day-old SPF chickens infected with avian infectious anemia virus mainly exhibited symptoms such as lethargy, reduced feed intake, and pale combs. The weight of the challenge model group (Group II) was significantly lower than that of the control group (Group I). The Danggui Buxue Tang group showed slightly better results than the Qingwen Baidu San group, indicating that tonifying qi and blood is beneficial for improving the metabolic capacity of the gastrointestinal and bone marrow systems. The superiority of Group VI over Group V is speculated to be related to the increased gastrointestinal absorption and utilization of active ingredients after enzymatic extraction of the drug.

[0077] 2.2 Infection indicators: Infectious dose is 10000 EID 50At that time, the mortality rate of SPF chickens in the 1-day-old challenge model group was as high as 63.3%, with most deaths occurring 14 to 18 days after infection. The mortality rates of groups III to VI decreased to varying degrees, especially in the Qingwen Baidu San and patent example groups. The decrease in mortality rate in the Danggui Buxue Tang group was not significant, indicating that after chickens are infected with infectious anemia virus, toxemia leads to circulatory system disorders and organ failure. Simply increasing qi and blood replenishment cannot counteract viral damage; it is necessary to use pathogen-removing agents to fight the virus. The mortality rate of the patent example group was significantly lower than that of the Qingwen Baidu San group, indicating that the invention example group is more effective in addressing the pathogenesis of chicken infectious anemia virus-induced blood deficiency. Hematocrit results showed that after viral challenge, hematocrit dropped to about 15%. After medication, the effect of Dang Gui Bu Xue Tang (Angelica Blood-Nourishing Decoction) was better than that of Qing Wen Bai Du San (Clear Heat and Detoxify Powder) group, but the difference was large compared with the blank control group. After using the patent implementation group, hematocrit rose to about 28%, which corresponded to the clinical observation of reddening of the crown beard and increased food intake. Moreover, for the thymus, which is the site with the highest viral load, the effect (viral load value) of the patent implementation group and Qing Wen Bai Du San group (which use tonifying and eliminating pathogens) was significantly better than that of the tonifying Dang Gui Bu Xue Tang group. The patent implementation group (which uses tonifying and eliminating pathogens) was better than the Qing Wen Bai Du San group (which simply clears heat and drains fire without tonifying and nourishing the body).

[0078] Experiment 2

[0079] A poultry farm in Handan City, Hebei Province, raised 30,000 Hy-Blue commercial laying hens (two chicken houses, 15,000 hens in each house). At 342 days old, the flock began to show signs of loss of appetite, pale skin (especially comb and wattles), emaciation, thin blood, longer clotting time, paler plasma than normal, and decreased egg production (egg rate decreased by 3-5%). The daily mortality rate increased from 0.1% to 0.5%.

[0080] Necropsy revealed that the organs of dead and diseased chickens were atrophied (liver, kidneys, spleen, etc.), the muscles and internal organs were pale, the bone marrow was atrophied, the bone marrow of the thigh bone was fat-colored and pale yellow, and the thymus was atrophied and dark reddish-brown.

[0081] The resident veterinarian took blood samples from the sick chickens for testing. The hematocrit was significantly lower than normal (around 17%), and the test was positive for chicken infectious anemia virus. After ruling out avian adenovirus group I infection, folic acid deficiency, sulfonamide poisoning, avian toxoplasmosis, and other infections, the diagnosis was chicken infectious anemia virus infection.

[0082] To prevent the spread of the disease, while ensuring environmental disinfection, 162 infected chickens were isolated and raised separately. Both the main flock and the isolated sick chickens were treated with the oral solution described in Example 2 of the invention, at a dosage of 1000ml diluted in 600kg of water for 5 consecutive days. Results showed that on the 4th day of treatment, the main flock's feed intake significantly increased (from an average of 100g per bird per day to approximately 125g), and the daily mortality rate decreased from approximately 150 birds to 26 birds on the 5th day (close to the normal mortality rate). Seven days after the treatment ended, the paleness of the combs, wattles, and visible mucous membranes in the main flock significantly improved, and the egg production rate recovered to approximately 91% (a 4% increase compared to before treatment).

[0083] 162 sick chickens were isolated and fed the same dosage in drinking water for 3-4 hours for 5 days. Their feed intake and comb and wattle color recovered significantly. Necropsy revealed that the visceral atrophy of 5 chickens had significantly improved, blood clotting time was close to normal, and hematocrit had risen to about 33% (close to normal). Seven days after the treatment, 10 chickens were randomly sampled for testing. Seven chickens tested negative for infectious anemia virus, and three tested positive. The treatment was continued at the same dosage for 3 days. Seven days after the treatment, 10 chickens were randomly sampled for testing. All 10 chickens tested negative for infectious anemia virus. The customer reported that the effect was satisfactory.

[0084] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions above are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed.

Claims

1. A traditional Chinese medicine composition for preventing and treating infectious anemia in chickens, characterized in that, The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20-40 parts of black vine, 10-30 parts of yellow root, 10-30 parts of hematite, 10-20 parts of fern root, and 10-20 parts of red baby's foot.

2. The traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to claim 1, characterized in that, The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30 parts of black vine, 20 parts of yellow root, 20 parts of hematite, 15 parts of fern root, and 15 parts of red baby's foot.

3. The traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to claim 1 or 2, characterized in that, The dosage form of the traditional Chinese medicine composition is a powder or oral liquid.

4. The traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to claim 3, characterized in that, The dosage form is an oral liquid, which also includes sodium benzoate and nipagin.

5. The traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to claim 4, characterized in that, The amount of sodium benzoate used is 0.1% to 0.2% of the total weight of the active pharmaceutical ingredient; the amount of paraben used is 0.05% to 0.1% of the total weight of the active pharmaceutical ingredient.

6. A method for preparing the traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to any one of claims 1 to 5, characterized in that, The raw materials of black vine, yellow root, hematite, fern root, and red baby's ear are mixed, pulverized, and prepared into corresponding dosage forms according to pharmaceutical requirements.

7. The method for preparing the traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to claim 6, characterized in that, The dosage form of the traditional Chinese medicine composition is an oral liquid, which is prepared using a steam explosion extraction-pressure filtration-concentration preparation technology.

8. The method for preparing the traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to claim 7, characterized in that, The steps are as follows: (1) Crush black vine, yellow root, hematite, fern root, and red baby into coarse powder, and then crush them into fine powder by steam explosion; (2) Take the fine powder obtained in step (1) and add 8 to 10 times the volume of water for extraction and decoction for the first time, and add 6 to 7 times the volume of water for extraction and decoction for the second time; filter and separate, concentrate the separated medicinal liquid to the weight of the medicinal liquid to 1.0 ± 0.1 times the total weight of the Chinese herbal raw materials, add 0.1% to 0.2% sodium benzoate and 0.05% to 0.1% paraben to the concentrated medicinal liquid to obtain oral liquid.

9. The method for preparing the traditional Chinese medicine composition for preventing and treating infectious anemia in chickens according to claim 8, characterized in that, In step (1), the steam pressure is 4-6 MPa and the time is 5-8 minutes.

10. The preparation method according to claim 8, characterized in that, In step (2), the initial decocting temperature is 85-90℃ and the decocting time is 30-60 min. The second decocting temperature is 60-65℃ and the decocting time is 10-30 min.

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