Application of plant extract composition in cleaning intestinal waste and moistening bowel and laxative products

Abstract

The present invention belongs to the field of plant extract compositions, and is mainly related to products containing plant extract compositions, and is particularly related to the application of plant extract compositions in cleaning intestinal garbage and laxative products. The present invention uses plant lactobacillus CICC-24936 and acidophilus Lactobacillus CICC-6095 to ferment Euryale ferox, sunflower disk, papaya hydrolysate and gardenia, and is matched with red raspberry juice, sour cherry juice, hawthorn, mulberry leaf, chicory, kudzu root, celery seed, eucommia male flower and corn silk to make preparations, which have the functions of cleaning intestinal garbage toxins and laxative. Treat constipation, lose weight, control weight, reduce uric acid, dissolve tophi, relieve joint pain, inhibit sugar absorption, diuresis and detumescence, lower blood pressure, lower blood lipids, lower blood sugar, invigorate the stomach and digest food, clear away heat and remove fire, promote the growth of bifidobacteria, improve intestinal function, sober up and sober up, improve sleep, anti-thrombosis, anti-atheroma, beauty spot lightening, anti-oxidation, reduce the effects of body burden.

Description

Technical Field

[0001] The invention belongs to the field of plant extract compositions, and mainly relates to products containing the plant extract compositions, and in particular to the application of the plant extract compositions in products for cleaning intestinal waste and moistening the intestines and relieving constipation. Background Art

[0002] If harmful substances discharged during the metabolism of the human body cannot be "cleaned" in time, it will easily lead to a decline in human body functions and induce diseases. 60% of the stool is water, and the rest is indigestible food residues, bacteria, bile pigments and electrolytes, which are not easy to be discharged. The human body has two "golden times" for defecation: one is the "standing reflex" when getting up in the morning. In the morning, when you change from lying to sitting and then standing, the brain will send a message to the large intestine to make the intestine move, and the intestine will help you push out the stool. The other is the "stomach-large intestine reflex" after breakfast. After eating, it notifies the large intestine to move quickly, creating the "right time" for defecation. Usually the large intestine will move three to four times a day, pushing waste from the colon to the rectum, and the expansion of the rectum will produce the urge to defecate. If you hold back the urge to defecate all day long, food residues will be retained in the folds of the intestines. They will rot and ferment under the action of bacteria. Over time, water will be absorbed and constipation will occur. The thick and heavy toxic substances in these food residues will adhere to the intestinal wall like rust, which is called stool retention. Stool retention constantly produces various toxins and toxic gases, causing intestinal environmental disorders and gastric dysfunction, which will lead to endocrine disorders and may also cause other diseases such as colon cancer.

[0003] Occasional constipation and constipation of bedridden people can use some measures to assist defecation, such as lubricating the anus with enema and glycerin suppositories to assist in the discharge of feces. Even stimulant laxatives can be used to treat acute constipation and clean the intestines. However, "medicine is three-point poison", so these laxatives are not recommended for long-term consumption by patients. At present, the main ingredients of many laxatives are rhubarb, senna leaves, etc., which can cause colitis and are not suitable for long-term use. In fact, some plants also have certain effects of clearing intestinal garbage and moistening the intestines and relieving constipation. For example, natural plants such as Euryale ferox, sunflower disk and gardenia have laxative effects. Therefore, the present invention is based on this, and through research on various plants, mulberry leaves, Euryale ferox, sunflower disk, hawthorn, papaya, chicory, gardenia, kudzu root, celery seeds, Eucommia male flowers and corn silk are selected as raw materials, and a plant extract composition and product with the effects of cleaning intestinal garbage, moistening the intestines and relieving constipation and enriching blood are developed through biological fermentation technology. Summary of the invention

[0004] In view of this, the present invention selects Euryale ferox, sunflower disc, gardenia and papaya hydrolysates to improve performance through segmented fermentation with Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095, so as to achieve the effects of cleaning intestinal waste, moistening the intestine and relieving constipation and replenishing blood.

[0005] In a first aspect, the present invention provides a plant extract composition, which is obtained by fermenting and extracting a composite strain; the composition comprises 5-7 parts of Euryale ferox, 12-14 parts of sunflower discs, 10-15 parts of Gardenia jasminoides and 20-25 parts of papaya hydrolysate by weight; the composite strain comprises Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095.

[0006] In the second aspect, the application of the plant extract composition in cleaning intestinal waste and moistening the intestines and relieving constipation, and the application of the plant extract composition in preparing functional products have the effects of cleaning intestinal waste, moistening the intestines and relieving constipation and replenishing blood.

[0007] The functional product provided by the present invention has the following specific steps for its preparation:

[0008] S1. Raw material pretreatment: Select fresh and high-quality mulberry leaves, Euryale ferox, sunflower disc, hawthorn, papaya, chicory, gardenia, kudzu root, celery seeds, Eucommia male flowers and corn silk, wash them with water, dry them, crush them and set aside; weigh 3 mL of red raspberry juice and 3 mL of sour cherry juice, where red raspberry juice (25.25 kg / barrel) and sour cherry juice (20.19 kg / barrel) were purchased from Sanmenxia Yingrun Food Co., Ltd.;

[0009] S2, preparation of papaya hydrolysate: take 15g of papaya powder pretreated in step S1, add 150mL of water to dissolve, put it in a water bath, and extract it with hot water at 90°C for 3h to obtain papaya extract; then adjust pH=5 at 50°C, add 8g of papain to hydrolyze for 3h, after the reaction, place it in a water bath at 85°C for 10 min to inactivate the enzyme, and cool it to obtain papaya hydrolysate;

[0010] S3. Preparation of bacterial liquid: Lactobacillus plantarum CICC-24936 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus plantarum, which was then transferred to a shake flask containing 500-1000 mL of MRS liquid medium and fermented for 6 h to obtain a concentration of 1.2-1.5×10 8 cfu / mL of Lactobacillus plantarum; Lactobacillus acidophilus CICC-6095 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus acidophilus, which was then transferred to a shake flask containing 500-1000 mL MRS liquid medium and fermented for 6 h to obtain a concentration of 1.2-1.5×10 8 cfu / mL Lactobacillus acidophilus bacterial liquid; Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095 were purchased from China Industrial Microbiological Culture Collection Management Center;

[0011] S4, plant fermentation: 5-7g of Euryale ferox, 12-14g of sunflower disc, 10-15g of Gardenia jasminoides in step S1 and 20-25g of papaya hydrolysate in step S2 are mixed to obtain a composition, the combination is ultrasonicated for 5-10min, 10-20mL of plant lactobacillus liquid prepared in step S3 is added, and aerobic fermentation is performed at 37-39°C for 12-24h to obtain a first product; then 10-20mL of Lactobacillus acidophilus liquid is added, and anaerobic fermentation is performed at 37-39°C for 12-24h to complete the fermentation; after the fermentation is completed, 3% H2O2 solution is added to inactivate plant lactobacillus and Lactobacillus acidophilus to obtain a plant fermentation product;

[0012] S5, extraction of fermented product: take 40 mL of the plant fermented product in step S4, adjust pH=2.0, add 60 mL of 70% ethanol, perform ultrasonic extraction for 35 min, and then perform reflux extraction for 30-60 min through a condensation reflux device. After extraction, collect the supernatant by suction filtration. The collected supernatant is kept at a temperature of 55° C., adjust pH=9.0, add 0.1 mL of alkaline protease, and stir and hydrolyze for 30 min. After the hydrolysis is completed, concentrate. When the concentration reaches 70-80% of the original volume, a high-purity plant extract fermentation liquid, i.e., a plant extract composition, can be obtained;

[0013] S6, preparation of the preparation: take 20 mL of the plant extract composition prepared in step S5, 5-10 g of hawthorn, 3-6 g of mulberry leaves, 4-8 g of chicory, 2-5 g of eucommia male flowers, 3-5 g of celery seeds, 15-30 g of kudzu root and 2-5 g of corn silk in step S1, as well as 3 mL of red raspberry juice and 3 mL of cherry juice, add 200-300 mL of water, put into a blender and stir for 15 min, then add L-arabinose, fructose syrup, citric acid and sodium citrate, stir for another 5 min, filter, and obtain the preparation; the L-arabinose selected in this step is a low GI composite sugar, has weight loss and blood sugar lowering effects, and is relatively friendly to obese and diabetic patients;

[0014] S7, Sterilization: The preparation is sterilized at high temperature at 135°C for 10-20 seconds. Compared with the general sterilization method, this step uses high temperature instantaneous sterilization to obtain smaller molecular clusters, which enhances the permeability, solubility and diffusion of the preparation.

[0015] S8. Canning: Take 180-200mL of the preparation and fill it into a packaging bottle made of PP (polypropylene) material, and then use an automatic packaging machine to seal it. When packaging, it is necessary to observe whether the liquid output meets the packaging volume, paste the packaging label, check the swab and prescription information and packaging volume again, and you can get the bottled preparation after confirmation. The packaging bottle made of PP material in this step has the characteristics of thermoplasticity, light specific gravity, and chemical corrosion resistance. It can be placed in a microwave oven for heating before drinking, which is more friendly to the elderly and children with poor intestinal function.

[0016] Preferably, the raw materials selected in step S1 of the present invention, namely, mulberry leaves, euryale seeds, sunflower discs, hawthorns, papayas, chicory, gardenias, kudzu roots, celery seeds, eucommia male flowers and corn silks, are all fresh and high-quality raw materials selected manually.

[0017] Preferably, the water used in the present invention is deionized water prepared by RO reverse osmosis.

[0018] Preferably, the papaya selected in step S1 of the present invention is medicinal papaya.

[0019] Preferably: the components of the MRS agar medium used in step S3 of the present invention are: 10 g peptone, 10 g beef extract powder, 5 g yeast extract powder, 20 g glucose, 1 g Tween, 5 g sodium acetate, 2 g triammonium citrate, 2 g dipotassium hydrogen phosphate, 0.2 g magnesium sulfate heptahydrate, 0.05 g sulfuric acid tetrahydrate, 15 g agar, 1000 mL distilled water, and the pH is adjusted to 6.2-6.5.

[0020] Preferably: the volume of the MRS liquid culture medium selected in step S3 of the present invention is 800 mL.

[0021] Preferably, in step S4 of the present invention, the amount of Lactobacillus plantarum liquid and Lactobacillus acidophilus liquid added is 15 mL.

[0022] Preferably, in step S6 of the present invention, the added amounts of L-arabinose, fructose syrup, citric acid and sodium citrate are 3 g, 2 g, 0.3 g and 0.1 g respectively.

[0023] Preferably, in step S7 of the present invention, the time for sterilizing the preparation in the can is 16 seconds.

[0024] The present invention has the following beneficial effects:

[0025] The present invention uses plant lactobacillus CICC-24936 and acidophilus Lactobacillus CICC-6095 to perform segmented fermentation on natural plants Euryale ferox, sunflower disc and gardenia to improve performance, thereby achieving the effects of cleaning intestinal waste and moistening the intestines and relieving constipation.

[0026] The papaya hydrolysate and gardenia jasminoides have the effect of nourishing blood after being synergistically fermented.

[0027] The present invention uses L-arabinose, which is a low GI compound sugar, has the effect of reducing weight and lowering blood sugar, and is more friendly to obese and diabetic patients;

[0028] 4. The present invention uses high-temperature instantaneous sterilization to obtain smaller molecular clusters, which can enhance the permeability, solubility and diffusion of the preparation.

[0029] 5. The packaging bottle of the present invention uses PP material, which has the characteristics of thermoplasticity, light specific gravity, chemical corrosion resistance, etc. It can be heated in a microwave oven before drinking, which is more friendly to the elderly and children with poor intestinal function. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0031] Figure 1 It is a comparison chart of the intestinal propulsion rate of each group of mice after taking the preparations of Example 2 and Comparative Examples 4-8.

[0032] Figure 2 This is the feces picture of the blank group mice.

[0033] Figure 3 This is the feces picture of mice in the model group.

[0034] Figure 4 This is a picture of the feces of mice in Group 2 of Example 2.

[0035] Figure 5 This is a picture of the feces of mice in group 4 of the comparative example.

[0036] Figure 6 This is a picture of the feces of mice in group 5 of the comparative example.

[0037] Figure 7 This is a picture of the feces of mice in group 6 of the comparative example.

[0038] Figure 8 This is a picture of the feces of mice in group 7 of the comparative example.

[0039] Fig. 9 This is a picture of the feces of mice in group 8 of the comparative example.

[0040] Fig.10 This is the feces picture of mice in the positive control group.

[0041] Fig.11This is the nuclear magnetic resonance spectrum of Example 3.

[0042] Fig.12 It is the nuclear magnetic resonance spectrum of comparative example 9.

[0043] Fig.13 This is a physical picture of the product of Example 3.

[0044] Fig.14 This is a physical picture of the product production line of Example 3. DETAILED DESCRIPTION

[0045] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0046] Example 1

[0047] This example proves that the enzymatically modified papaya hydrolysate and Gardenia jasminoides have the effect of nourishing blood after synergistic fermentation.

[0048] This embodiment provides a plant extract composition and its application, and the specific steps are as follows:

[0049] S1. Raw material pretreatment: Select fresh and high-quality mulberry leaves, Euryale ferox, sunflower disc, hawthorn, papaya, chicory, gardenia, kudzu root, celery seeds, Eucommia ulmoides male flowers and corn silk, wash them with water, dry them, crush them and set aside; weigh 3 mL of red raspberry juice and 3 mL of sour cherry juice; red raspberry juice (25.25 kg / barrel) and sour cherry juice (20.19 kg / barrel) were purchased from Sanmenxia Yingrun Food Co., Ltd.;

[0050] S2, preparation of papaya hydrolysate: 15 g of papaya powder pretreated in step S1 was taken, 150 mL of water was added to dissolve, and the mixture was placed in a water bath, and hot water was extracted at 90° C. for 3 h to obtain papaya extract; then, at 50° C., the pH was adjusted to 5, 8 g of papain was added to hydrolyze for 3 h, and after the reaction was completed, the mixture was placed in a water bath at 85° C. for 10 min to inactivate the enzyme, and the papaya hydrolysate was obtained after cooling;

[0051] S3. Preparation of bacterial liquid: Lactobacillus plantarum CICC-24936 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus plantarum. The activated Lactobacillus plantarum was transferred to a shake flask containing 800 mL of MRS liquid medium and fermented for 6 h to obtain a concentration of 1.2×10 8cfu / mL of Lactobacillus plantarum liquid; Lactobacillus acidophilus CICC-6095 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus acidophilus, which was then transferred to a shake flask containing 800 mL of MRS liquid medium and fermented for 6 h to obtain a concentration of 1.2×10 8 cfu / mL Lactobacillus acidophilus bacterial liquid; Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095 were purchased from China Industrial Microbiological Culture Collection Management Center;

[0052] S4, plant fermentation: 5 g of Euryale ferox, 12 g of sunflower disc, 10 g of Gardenia jasminoides in step S1 and 20 g of papaya hydrolysate in step S2 were mixed to obtain a composition, the composition was ultrasonicated for 5 min, 15 mL of the plant lactobacillus liquid prepared in step S3 was added, and aerobic fermentation was performed at 37° C. for 12 h to obtain a first product; then 15 mL of the Lactobacillus acidophilus liquid was added, and anaerobic fermentation was performed at 37° C. for 12 h to complete the fermentation; after the fermentation was completed, 3% H2O2 solution was added to inactivate the plant lactobacillus and the Lactobacillus acidophilus to obtain a plant fermentation product;

[0053] S5, extraction of fermentation product: take 40 mL of the plant fermentation product in step S4, adjust pH=2.0, add 60 mL of 70% ethanol, perform ultrasonic extraction for 35 min, and then perform reflux extraction for 30 min through a condensation reflux device. After the extraction, filter and collect the supernatant. The collected supernatant is kept at 55° C. After the extraction, the temperature is maintained at 55° C., adjust pH=9.0, add 0.1 mL of alkaline protease, stir and hydrolyze for 30 min, and concentrate after the hydrolysis is completed. When the volume is concentrated to 70% of the original volume, a high-purity plant extract fermentation liquid, i.e., a plant extract composition, can be obtained.

[0054] Comparative Example 1: Except that papaya hydrolysate is not added in step S4, the remaining steps are the same as those in Example 1.

[0055] Comparative Example 2: Except that papaya extract is directly used without enzymatic hydrolysis of papaya in step S2, the remaining steps are the same as those in Example 1.

[0056] Comparative Example 3: Except that Gardenia jasminoides is not added in step S4, the remaining steps are the same as those in Example 1.

[0057] Experiment 1: Grouping: 42 SPF male mice were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd. and randomly divided into 7 groups, 6 mice in each group, namely the normal group (injected with equal volume of normal saline), the model group, the Example 1 group, the comparative example 1-3 groups, and the positive control group (2.7 g / kg dose of regenerative blood tablets); except the normal group, the mice in other groups were subjected to modeling treatment.

[0058] Modeling scheme of aplastic anemia mouse model: except for the normal group mice, the other groups of mice were intraperitoneally injected with 10 mL / kg busulfan solution 10 mg / kg on the 1st, 3rd, 5th, 7th, 9th, and 11th days; 10 mL / kg cyclophosphamide 20 mg / kg was intraperitoneally injected on the 2nd, 4th, 6th, 8th, 10th, and 12th days. After the preparation of the aplastic anemia model, the Example 1 group and the Comparative Examples 1-3 groups were respectively gavaged with 20 mL / kg of the corresponding plant extract composition, the positive control group was gavaged with 2.7 g / kg of regeneration blood tablets every day, the model group was injected with an equal volume of saline, and the normal group was injected with an equal volume of saline for normal mice, and the administration was continued for 30 days; after the last administration, fasting but not water for 12 hours, then the eyeballs were removed for blood collection, heparin sodium anticoagulation, and the number of peripheral blood red blood cells (RBC), white blood cells (WBC), platelets (PLT) and hemoglobin (HGB) content were determined by a fully automatic biochemical analyzer. The results are shown in Table 1.

[0059] Table 1

[0060]

[0061] Table 1 is a graph showing the results of Example 1 and Comparative Examples 1-3 after alternating intraperitoneal injection of busulfan 10 mg / kg and cyclophosphamide 20 mg / kg for 12 consecutive days. mg / kg after the peripheral blood red blood cells (RBC), white blood cells (WBC), platelets (PLT) number and hemoglobin (HGB) content were compared. It can be seen that compared with the normal group of mice, the model group of mice peripheral blood red blood cells (RBC), white blood cells (WBC), platelets (PLT) number and hemoglobin (HGB) content were significantly reduced; compared with the model group of mice, the example 1 group of mice peripheral blood red blood cells (RBC), white blood cells (WBC), platelets (PLT) number and hemoglobin (HGB) content were significantly increased, while the comparison example 1 group, comparison example 2 group and comparison example 3 group of mice peripheral blood red blood cells (RBC), white blood cells (WBC), platelets (PLT) number and hemoglobin (HGB) content did not change significantly, confirming that the enzymatically modified papaya hydrolysate and gardenia have the effect of enriching blood after synergistic fermentation.

[0062] Example 2

[0063] This example demonstrates that the segmented fermentation of Euryale ferox, sunflower disc and Gardenia jasminoides using Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095 improves the performance and has the effects of cleaning intestinal waste and moistening the intestines to promote bowel movements.

[0064] This embodiment provides the use of a plant extract composition in cleaning intestinal waste and moistening the intestines and relieving constipation, and the specific steps are as follows:

[0065] S1. Raw material pretreatment: select fresh and high-quality mulberry leaves, Euryale ferox, sunflower disc, hawthorn, papaya, chicory, gardenia, kudzu root, celery seeds, Eucommia ulmoides male flowers and corn silk, wash them with water, dry them in the air, crush them and set aside; weigh 3 mL of red raspberry juice and 3 mL of sour cherry juice;

[0066] S2, preparation of papaya hydrolysate: 15g of papaya powder pretreated in step S1 was taken, 150mL of water was added to dissolve, and the mixture was put into a water bath, and hot water was extracted at 90°C for 3h to obtain papaya extract; then, at 50°C, pH=5 was adjusted, 8g of papain was added for enzymolysis for 3h, and after the reaction was completed, the mixture was placed in a water bath at 85°C for 10 min to inactivate the enzyme, and the papaya hydrolysate was obtained after cooling; wherein Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095 were purchased from the China Industrial Microbiological Culture Collection Administration Center;

[0067] S3. Preparation of bacterial solution: Lactobacillus plantarum CICC-24936 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus plantarum. The activated Lactobacillus plantarum was transferred to a shake flask containing 800 mL MRS liquid medium and fermented for 6 h to obtain a concentration of 1.3×10 8 cfu / mL of plant lactobacillus liquid; Lactobacillus acidophilus CICC-6095 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus acidophilus, which was then transferred to a shake flask containing 800 mL of MRS liquid medium and fermented for 6 h to obtain a concentration of 1.3×10 8 cfu / mL Lactobacillus acidophilus liquid;

[0068] S4, plant fermentation: 6 g of Euryale ferox, 13 g of sunflower disc, 13 g of Gardenia jasminoides in step S1 and 22 g of papaya hydrolysate in step S2 were mixed to obtain a composition, the composition was ultrasonicated for 8 min, 15 mL of the plant lactobacillus solution prepared in step S3 was added, and aerobic fermentation was performed at 38° C. for 18 h to obtain a first product; then 15 mL of the Lactobacillus acidophilus solution was added, and anaerobic fermentation was performed at 38° C. for 18 h to complete the fermentation; after the fermentation was completed, 3% H2O2 solution was added to inactivate the plant lactobacillus and the Lactobacillus acidophilus to obtain a plant fermentation product;

[0069] S5, extraction of fermentation product: take 40 mL of the plant fermentation product in step S4, adjust pH=2.0, add 60 mL of 70% ethanol, perform ultrasonic extraction for 35 min, and then perform reflux extraction for 45 min through a condensation reflux device. After extraction, collect the supernatant by suction filtration. The collected supernatant is kept at a temperature of 55° C., adjust pH=9.0, add 0.1 mL of alkaline protease, stir and hydrolyze for 30 min, concentrate after hydrolysis, and concentrate to 75% of the original volume to obtain a high-purity plant extract fermentation liquid, i.e., a plant extract composition;

[0070] Comparative Example 4: The four plants (Gorgon fruit, sunflower disc, Gardenia, and papaya hydrolysate) were fermented and modified without using biological fermentation technology, that is, the mixed solution obtained by dissolving the Gorgon fruit, sunflower disc, Gardenia, and papaya hydrolysate in deionized water in step S4 was not fermented by Lactobacillus plantarum and Lactobacillus acidophilus, and the remaining steps were the same as Example 2.

[0071] Comparative Example 5: In step S4, four plants (euryale ferox, sunflower disc, gardenia, papaya hydrolysate) were fermented in stages using Lactobacillus plantarum CICC-24936 alone, and the remaining steps were the same as those in Example 2.

[0072] Comparative Example 6: In step S4, Lactobacillus acidophilus CICC-6095 was used alone to perform segmented fermentation on four plants (euryale ferox, sunflower disc, gardenia, and papaya hydrolysate), and the remaining steps were the same as those in Example 2.

[0073] Comparative Example 7: In step S4, Lactobacillus plantarum CICC-22134 (purchased from China Industrial Microbiological Culture Collection Center) was used to replace Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095 to perform segmented fermentation on four plants (euryale ferox, sunflower disc, gardenia, and papaya hydrolysate), and the remaining steps were the same as in Example 2.

[0074] Comparative Example 8: In step S4, Lactobacillus acidophilus CICC-20248 (purchased from China Industrial Microbiological Culture Collection Center) was used to replace Lactobacillus acidophilus CICC-6095 and Lactobacillus plantarum CICC-24936 to perform segmented fermentation on four plants (euryale ferox, sunflower disc, gardenia, and papaya hydrolysate), and the remaining steps were the same as in Example 2.

[0075] Experiment 2: Effects of plant extract composition on constipation model mice

[0076] SPF grade Balb / c male mice (provided by Beijing Weitonglihua Experimental Animal Technology Co., Ltd.), 100 days old, weighing 18-22g each. The animals were raised in an environment with 12h light and dark alternation, constant temperature of 25±2℃ and humidity of 50%±10%, with free access to water and food. The experiment began after 54 mice were adaptively raised for 5 days and randomly divided into a modeling group of 48 mice and a blank group of 6 mice; the blank group mice were given 0.2 mL / (10 g·d) of pure water by gavage for 4 consecutive days.

[0077] Constipation model establishment: The modeling group mice were given 8 mg / (kg·d) of loperamide suspension with a concentration of 0.4 mg / mL by gavage for 4 consecutive days, once a day. On the 4th day after modeling, the number of feces particles and the wet weight of feces in the mice after 6 hours were significantly reduced compared with those before modeling. The modeling was successful. The 48 mice with successful modeling were randomly divided into 8 groups, 6 mice in each group, namely the model group, Example 2 group, Comparative Example 4-8 groups, and the positive control group. Starting from the 5th day of the experiment, the model group, Example 2 group, Comparative Example 4-8 groups, and the positive control group were still gavaged with the above-mentioned loperamide suspension and the dosage for a total of 4 days. 2 hours after daily gavage, the mice in Example 2 group were gavaged with 0.2 mL / (10 g·d) of the plant extract composition of Example 2 group, and the mice in Comparative Example 4-8 groups were gavaged with 0.2 mL / (10 g·d) of the corresponding plant extract composition of Comparative Example 4-8 groups. The positive control group was gavaged with 0.6 g / (kg·d) of a 30 mg / ml raw rhubarb suspension, and the mice in the blank group and the model group were gavaged with 0.2 mL / (10 g·d) of pure water. Each group was gavaged once a day for 4 consecutive days.

[0078] Determination of fecal pellet count and wet weight of feces in each group of mice within 6 hours: On the day after the last oral gavage, mice were housed in individual cages and allowed to drink water freely. Feces were collected within 6 hours using EP tubes. The fecal pellet count and wet weight of mice in each group were recorded within 6 hours. The feces of one mouse in each group was selected as shown in the figure below. Figure 2-10 The experimental results are shown in Table 2 below.

[0079] Table 2

[0080]

[0081] Table 2 is a comparison chart of mouse feces. The results show that the mice in the blank group have always maintained a good mental state, shiny fur, and are lively and active; compared with the blank group, the number of feces particles and wet weight of feces of the mice in the model group were significantly reduced; compared with the model group, the number of feces particles and wet weight of feces of the mice in Example 2 and Comparative Examples 4-8 were increased, and the number of feces particles and wet weight of feces of the mice in Example 2 were higher than those in Comparative Examples 4-8, and the number of feces particles and wet weight of feces of the mice were slightly higher than those in the positive control group.

[0082] Determination of intestinal propulsion rate of mice in each group: weigh 100 g of gum arabic and put it into a 1L beaker, add 800 mL of pure water, stir well and put it into a boiling pot to boil, keep stirring at a constant speed during the process, when the solution in the pot boils to a transparent state, add 50 g of activated carbon powder into the boiling pot, boil and cool to room temperature, boil without adding water for 3 times, wait for the solution to cool naturally, add distilled water to 1L, and store in a refrigerator at 4℃. The next day after the last gavage, after recording the feces of mice, each group of mice fasted for 6 hours but not water, and then gavaged with 0.4 mL of ink, and killed 20 minutes later, opened the abdominal cavity, fully exposed and separated the mesentery, cut the intestinal tube from the pylorus to the ileocecal part, flattened on the experimental table without gravity, and gently pulled the small intestine into a straight line to detect the intestinal propulsion rate of the small intestine. The total length of the intestinal tube is L1, the distance traveled by the ink in the small intestine is L2, and the intestinal propulsion rate = L2 / L1×100%.

[0083] Figure 1 It is a comparison chart of the intestinal propulsion rate of each group of mice. The results show that compared with the blank group, the intestinal propulsion rate of the model group mice was reduced; compared with the model group, the intestinal propulsion rate of the mice in Example 2 and the comparative example 4-8 groups were increased, and the propulsion rate of the mice in Example 2 group was significantly higher than that in the comparative example 4-8 groups and the positive control group.

[0084] In summary: the preparation of Example 2 can increase the amount of feces, wet weight of feces, and promote intestinal motility of constipation model mice, and are all higher than those of Comparative Examples 4-8. Therefore, Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095 are used to perform segmented fermentation on the hydrolysates of Euryale ferox, sunflower disc, Gardenia jasminoides and papaya to improve performance, thereby achieving the effect of clearing intestinal garbage toxins and moisturizing the intestines and promoting bowel movements.

[0085] Example 3

[0086] This example demonstrates that compared with the general sterilization method, the high temperature instantaneous sterilization in this step can obtain smaller molecular clusters and enhance the permeability, solubility and diffusion of the preparation.

[0087] This embodiment provides the use of a plant extract composition in cleaning intestinal waste and moistening the intestines and promoting bowel movements, and the specific steps are as follows:

[0088] S1. Raw material pretreatment: Select fresh and high-quality mulberry leaves, Euryale ferox, sunflower disc, hawthorn, papaya, chicory, gardenia, kudzu root, celery seeds, Eucommia ulmoides male flowers and corn silk, wash them with water, dry them, crush them and set aside; weigh 3 mL of red raspberry juice and 3 mL of sour cherry juice; red raspberry juice (25.25 kg / barrel) and sour cherry juice (20.19 kg / barrel) were purchased from Sanmenxia Yingrun Food Co., Ltd.;

[0089] S2, preparation of papaya hydrolysate: take 15g of papaya powder pretreated in step S1, add 150mL of water to dissolve, put it in a water bath, and extract it with hot water at 90°C for 3h to obtain papaya extract; then adjust pH=5 at 50°C, add 8g of papain to hydrolyze for 3h, after the reaction, place it in a water bath at 85°C for 10 min to inactivate the enzyme, and cool it to obtain papaya hydrolysate;

[0090] S3. Preparation of bacterial solution: Lactobacillus plantarum CICC-24936 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus plantarum. The activated Lactobacillus plantarum was transferred to a shake flask containing 800 mL MRS liquid medium and fermented for 6 h to obtain a concentration of 1.5×10 8 cfu / mL of Lactobacillus plantarum liquid; Lactobacillus acidophilus CICC-6095 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus acidophilus, which was then transferred to a shake flask containing 800 mL of MRS liquid medium and fermented for 6 h to obtain a concentration of 1.5×10 8 cfu / mL of Lactobacillus acidophilus bacterial liquid, and both bacterial liquids were placed in a bacterial liquid tank for standby use; wherein Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095 were purchased from the China Industrial Microbiological Culture Collection Management Center;

[0091] S4, plant fermentation: 7 g of Euryale ferox, 14 g of sunflower disc, 15 g of Gardenia jasminoides in step S1 and 25 g of papaya hydrolysate in step S2 were mixed to obtain a composition, the composition was ultrasonicated for 10 min, 20 mL of the plant lactobacillus solution prepared in step S3 was added, and aerobic fermentation was performed at 39° C. for 24 h to obtain a first product; then 20 mL of the Lactobacillus acidophilus solution was added, and anaerobic fermentation was performed at 39° C. for 24 h to complete the fermentation; after the fermentation was completed, 3% H2O2 solution was added to inactivate the plant lactobacillus and the Lactobacillus acidophilus to obtain a plant fermentation product;

[0092] S5, extraction of fermented product: take 40 mL of the plant fermented product in step S4, adjust pH=2.0, add 60 mL of 70% ethanol, perform ultrasonic extraction for 35 min, and then perform reflux extraction for 60 min through a condensation reflux device. After extraction, collect the supernatant by suction filtration. The collected supernatant is kept at a temperature of 55° C., adjust pH=9.0, add 0.1 mL of alkaline protease, stir and hydrolyze for 30 min, concentrate after hydrolysis, and concentrate to 80% of the original volume to obtain a high-purity plant extract fermentation liquid, i.e., a plant extract composition;

[0093] S6, preparation of the preparation: take 20 mL of the plant extract composition prepared in step S5, 8 g of hawthorn, 5 g of mulberry leaf, 6 g of chicory, 3 g of eucommia male flower, 4 g of celery seed, 23 g of kudzu root and 3 g of corn silk in step S1, as well as 3 mL of red raspberry juice and 3 mL of cherry juice, add 250 mL of water, put into a blender and stir for 15 min, then add 3 g of L-arabinose, 2 g of fructose syrup, 0.3 g of citric acid and 0.1 g of sodium citrate, stir for another 5 min, filter, and obtain the preparation; the L-arabinose selected in this step is a low GI composite sugar, has weight loss and blood sugar lowering effects, and is relatively friendly to obese and diabetic patients;

[0094] S7, Sterilization: The preparation is sterilized at high temperature at 135°C for 16 seconds. Compared with the general sterilization method, this step uses high temperature instantaneous sterilization to obtain smaller molecular clusters, which enhances the permeability, solubility and diffusion of the preparation.

[0095] S8, canning: take 190mL of the preparation and fill it into a packaging bottle made of PP material, and then use an automatic packaging machine to seal it. During packaging, it is necessary to observe whether the liquid output meets the packaging volume, paste the packaging label, check the swab and prescription information and the packaging volume again, and the bottled preparation can be obtained after confirmation. The packaging bottle made of PP material used in this step has the characteristics of thermoplasticity, light specific gravity, and chemical corrosion resistance. It can be placed in a microwave oven for heating before drinking, which is more friendly to the elderly and children with poor intestinal function. The product obtained in this embodiment is as follows Fig.13 As shown, the product production line is Fig.14 shown.

[0096] Comparative Example 9: Except for the general sterilization method in step S7, which uses a sterilization temperature of 100° C. for 16 seconds, the remaining steps are the same as those in Example 3.

[0097] Experiment 3: Using a 400MHz NMR spectrometer to measure 17 0 Spectral line width characterizes the average relative size of liquid water cluster structure. Parameter settings: acquisition pulse program zg, total number of scans (NS) 1024, frequency of ch.1 (01) 0, F1 channel -90 degree high frequency (P1) 12. The preparations obtained in Example 3 and Comparative Example 9 were tested, and their nuclear magnetic resonance spectra were as follows Figure 11-12 The results are shown in Table 3 below.

[0098] Table 3

[0099]

[0100] Table 3 is a comparison of the half width of the molecular clusters of the preparations of Example 3 and Comparative Example 9. It can be seen that the molecular clusters of the preparation of Example 3 are significantly lower than those of Comparative Example 9, and have stronger permeability, solubility and diffusion power.

[0101] The above-mentioned embodiments only express the specific implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present invention. It should be pointed out that, for those skilled in the art, without departing from the concept of the present invention, any other changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A plant extract composition, characterized in that: The invention discloses a composition obtained by fermenting and extracting composite strains; the composition is composed of 5-7 parts of euryale seeds, 12-14 parts of sunflower discs, 10-15 parts of gardenias and 20-25 parts of papaya hydrolysate by weight; the composite strains contain Lactobacillus plantarum CICC-24936 and Lactobacillus acidophilus CICC-6095; the preparation method of the papaya hydrolysate is as follows: 15 g of papaya powder is obtained by crushing air-dried papaya, 150 mL of water is added to dissolve the powder, the powder is put into a water bath, and the powder is extracted with hot water at 90°C for 3 hours to obtain a papaya extract; then, the pH is adjusted to 5 at 50°C, 8 g of papain is added for enzymolysis for 3 hours, and after the reaction is completed, the powder is placed in a water bath at 85°C for 10 minutes to inactivate the enzyme, and the papaya hydrolysate is obtained after cooling.

2. The plant extract composition according to claim 1, characterized in that: The plant extract composition has the effects of nourishing blood, clearing intestinal waste and moistening the intestines and facilitating bowel movements.

3. Use of the plant extract composition according to claim 1 or 2 in the preparation of products for cleaning intestinal waste and moistening the intestines and promoting bowel movements, characterized in that: The specific preparation method of the product is as follows: S1. Raw material pretreatment: select fresh and high-quality mulberry leaves, Euryale ferox, sunflower disc, hawthorn, papaya, chicory, gardenia, kudzu root, celery seeds, Eucommia male flowers and corn silk, wash them with water, dry them, crush them and set aside; weigh 3 mL of red raspberry juice and 3 mL of sour cherry juice and set aside; S2, preparation of papaya hydrolysate: 15 g of papaya powder pretreated in step S1 was taken, 150 mL of water was added to dissolve, and the mixture was placed in a water bath, and hot water was extracted at 90° C. for 3 h to obtain a papaya extract; then, the pH was adjusted to 5 at 50° C., 8 g of papain was added to hydrolyze for 3 h, and after the reaction was completed, the mixture was placed in a water bath at 85° C. for 10 min to inactivate the enzyme, and the papaya hydrolysate was obtained after cooling; S3. Preparation of bacterial liquid: Lactobacillus plantarum CICC-24936 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus plantarum. The activated Lactobacillus plantarum was transferred to a shake flask containing 500-1000 mL MRS liquid medium and fermented for 6 h to obtain a concentration of 1.2-1.5×10 8 cfu / mL of Lactobacillus plantarum; Lactobacillus acidophilus CICC-6095 was inoculated into MRS agar medium and cultured in a 37°C incubator for 48 h to obtain activated Lactobacillus acidophilus, which was then transferred to a shake flask containing 500-1000 mL MRS liquid medium and fermented for 6 h to obtain a concentration of 1.2-1.5×10 8 cfu / mL Lactobacillus acidophilus liquid; S4, plant fermentation: 5-7g of Euryale ferox in step S1, 12-14g of sunflower disc, 10-15g of Gardenia jasminoides and 20-25g of papaya hydrolysate in step S2 are mixed to obtain a composition, the composition is ultrasonicated for 5-10min, 10-20mL of plant lactobacillus liquid prepared in step S3 is added, and aerobic fermentation is performed at 37-39°C for 12-24h to obtain a first product, and then 10-20mL of Lactobacillus acidophilus liquid is added, and anaerobic fermentation is performed at 37-39°C for 12-24h to complete the fermentation; after the fermentation is completed, 3% H2O2 solution is added to inactivate plant lactobacillus and Lactobacillus acidophilus to obtain a plant fermentation product; S5, extraction of fermented product: take 40 mL of the plant fermented product in step S4, adjust pH=2.0, add 60 mL of 70% ethanol, perform ultrasonic extraction for 35 min, and then perform reflux extraction for 30-60 min through a condensation reflux device. After extraction, collect the supernatant by suction filtration. The collected supernatant is kept at a temperature of 55° C., adjust pH=9.0, add 0.1 mL of alkaline protease, and stir and hydrolyze for 30 min. After the hydrolysis is completed, concentrate. When the concentration reaches 70-80% of the original volume, a high-purity plant extract fermentation liquid, i.e., a plant extract composition, can be obtained; S6, preparation of the preparation: take 20 mL of the plant extract composition prepared in step S5, 5-10 g of hawthorn, 3-6 g of mulberry leaves, 4-8 g of chicory, 2-5 g of eucommia male flowers, 3-5 g of celery seeds, 15-30 g of kudzu root, and 2-5 g of corn silk in step S1, as well as 3 mL of red raspberry juice and 3 mL of cherry juice, add 200-300 mL of water, put into a blender and stir for 15 min, then add L-arabinose, fructose syrup, citric acid and sodium citrate, stir for another 5 min, filter, and obtain the preparation; S7, Sterilization: The preparation is subjected to high temperature instantaneous sterilization treatment, the sterilization temperature is 135°C, and the time is 10-20s; S8. Canning: Take 180-200mL of the preparation and fill it into a packaging bottle made of PP material, then use an automatic packaging machine to seal it. During packaging, it is necessary to observe whether the liquid output meets the packaging volume, paste the packaging label, check the swab and prescription information and packaging volume again, and you can get the bottled preparation after confirmation.

4. The use of the plant extract composition according to claim 3 in the preparation of products for cleaning intestinal waste and moistening the intestines and promoting bowel movements, characterized in that: The papaya selected in step S1 is medicinal papaya.

5. The use of the plant extract composition according to claim 4 in the preparation of products for cleaning intestinal waste and moistening the intestines and promoting bowel movements, characterized in that: The components of the MRS agar medium used in step S3 are: 10 g peptone, 10 g beef extract powder, 5 g yeast extract powder, 20 g glucose, 1 g Tween, 5 g sodium acetate, 2 g triammonium citrate, 2 g dipotassium hydrogen phosphate, 0.2 g magnesium sulfate heptahydrate, 0.05 g sulfuric acid tetrahydrate, 15 g agar, and 1000 mL distilled water, and the pH is adjusted to 6.2-6.

5.

6. Use of the plant extract composition according to claim 5 in preparing products for cleaning intestinal waste and moistening the intestines and promoting bowel movements, characterized in that: In the step S4, the amount of Lactobacillus plantarum liquid and Lactobacillus acidophilus liquid added is 15 mL.

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