A plant growth regulating composition comprising scutellarein and seminole

Abstract

The application discloses a plant growth regulating composition containing scutellarein and seminole, and belongs to the technical field of agricultural plant growth regulators. The mass ratio of scutellarein and seminole in the effective component of the plant growth regulating composition provided by the application is 1:30-13:1. It is proved that scutellarein and seminole have a synergistic effect when used for promoting crop growth and improving crop yield within the range of the ratio, and are particularly beneficial to improving the quality of crop fruits.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of plant growth regulators for agricultural plants, and discloses a plant growth regulator composition comprising scutellarin and hemiphorin. BACKGROUND

[0002] Scutellarin, also known as wild skyrin, high skyrin or scullcapflavone B, is the main active ingredient in short flying dandelion. At present, the research on the effect of scutellarin is mainly used for the treatment of ischemic cardiovascular and cerebrovascular diseases in clinical medicine. This drug has the effects of dilating blood vessels, improving blood microcirculation, reducing vascular resistance and resisting platelet aggregation. Patent CN102613185A describes that scutellarin can greatly promote the elongation of Arabidopsis thaliana roots at low concentrations. The promotion effect is enhanced with the increase of concentration within a certain concentration range, and when the maximum effect is reached, the effect is gradually weakened with the further increase of concentration. The root elongation promoting effect of scutellarin can be antagonized by hormone substances such as methyl jasmonate and auxin 2,4-D. The concentration-dependent effect mode and the characteristics of interaction with known hormones of scutellarin show that it regulates the process of root elongation in a similar hormone-like manner. At present, there is no report that scutellarin has the function of improving the yield (output) or fruit quality of crops.

[0003] Hemiphorin, also known as N-acetylthioproline and 3-acetylthiazolidine-4-carboxylic acid, has a protective effect on myocardial ischemia and is widely used in the medical field. In recent years, studies have found that hemiphorin can participate in plant signal transduction and play an important role, increase the activity of ATPase and other enzymes in plants, promote photosynthesis, and have the stress resistance characteristics of proline and the weak gibberellin efficacy. Exogenous hemiphorin treatment can significantly improve seed germination speed, enhance seed vigor, and improve seedling emergence rate. In agricultural production, it can also relieve the antagonism between phosphorus fertilizer and multi-element trace element fertilizer, stimulate the absorption and utilization of crops to fertilizers, and improve fertilizer efficiency.

[0004] Different plant growth regulators have different mechanisms of action, and mixing can exert synergistic effects on each other. For example, the combination of sodium nitrophenolate and naphthalene acetic acid can improve cell viability, accelerate plant growth rate, break seed dormancy, prevent flower and fruit drop, etc. The combination of sodium nitrophenolate and paclobutrazol can prolong plant cell division and elongation, shorten internodes, thicken stems, and compact plants, while also promoting flower bud formation, fruit retention, etc. Although numerous plant growth regulators have different molecular structures, they all have similar functions to plant endogenous hormones in terms of physiological functions. When two or more plant growth regulators act on plants, they will affect the synthesis, decomposition and activity of plant endogenous hormones, and this effect is a comprehensive performance of the synergistic action of multiple plant endogenous hormones. Therefore, when two or more plant growth regulators are mixed, the physiological effects of their combined action are often not equal to the sum of the effects of each individual action. That is, different types of plant growth regulators will interact during plant growth, and mixing can easily lead to unpredictable results. Therefore, when mixing plant growth regulators, scientific principles and methods should be followed to ensure safety and effectiveness. SUMMARY

[0005] In order to better exert the agricultural use of sculpin and semileaf in plant growth regulation, the present application provides a plant growth regulating composition containing sculpin and semileaf. The present application has carried out in-depth research and tests on the combination of sculpin and semileaf, and found that the combination of sculpin and semileaf can effectively improve and promote crop growth, and not only shows no antagonistic effect, but also shows synergistic effect within a certain range of combination ratio.

[0006] Specifically, the plant growth regulating composition containing sculpin and semileaf provided by the present application has an effective component for regulating plant growth and fruit, which is composed of sculpin and semileaf, and the mass ratio of sculpin to semileaf is 1:30-13:1.

[0007] It is easy for those skilled in the art to know that the combination or joint use of multiple plant growth regulators (pesticides) is one of the dominant trends in the future market of agricultural chemicals. However, in the case where the mechanism and mechanism of each component in the plant growth regulator (pesticide) composition are unknown, it is unpredictable whether the active ingredients of the plant growth regulator (pesticide) can produce synergistic effect. According to the comprehensive effect shown in the field test, the joint application of sculpin and semileaf has a yield-increasing effect on tomato, and the yield-increasing rate reaches 35.2-38.7% within the range of 1:30-13:1, which is higher than 10.3-25.0% of semileaf and 4.6-15.8% of sculpin, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate after the joint application of sculpin and semileaf is greater than 10%, showing synergistic effect.

[0008] Further preferably, the mass ratio of the scutellarein and the hemiptelein in the plant growth regulating composition is 1:5 to 4:1.

[0009] Further preferably, the mass ratio of the scutellarein and the hemiptelein in the plant growth regulating composition is 2:1.

[0010] It is easy for those skilled in the art to know that, in order to fully exert the pharmacological effect of the plant growth regulating active component, it can be prepared into a formulation dosage form convenient for application according to the need of application. The plant growth regulating composition is processed into an agriculturally acceptable formulation dosage form, and the formulation contains an auxiliary agent for assisting the scutellarein and the hemiptelein to regulate the growth and fruit of a plant. The plant growth regulating composition can be formulated into a water agent, a soluble liquid agent, a water dispersible granule, a wettable powder, a dispersible oil suspension agent, a suspension agent, a microemulsion, a granule, and a microcapsule agent with a certain slow-release effect, and the like. When the above different dosage forms are formulated, in addition to the need to use the active component for optional plant growth regulation, a person skilled in the art also needs to select a plurality of auxiliary agents, and different formulation auxiliary components (auxiliary agents) can be selected according to the need. The auxiliary components can be one or more of a dispersion medium, a dispersant, an emulsifier, a wetting agent, a thickening agent, an antifoaming agent, an antifreezing agent, a disintegrating agent, a binder, a filler, a carrier, and the like.

[0011] Further, the present application provides the use of the plant growth regulating composition containing the scutellarein and the hemiptelein in improving the quality of plant fruits and promoting the growth of plants. As described above, within the appropriate dosage ratio range, the plant growth regulating composition containing the scutellarein and the hemiptelein has an excellent effect of improving the quality of plant fruits, such as tomatoes and the like. Further preferably, the plant growth regulating composition containing the scutellarein and the hemiptelein and the preparation thereof have various application modes, and the present application is not particularly limited. Generally, according to the conventional application method of an exogenous plant growth regulator, the plant growth regulating composition containing the scutellarein and the hemiptelein is sprayed.

[0012] Regarding the use object of the plant growth regulating composition containing the scutellarein and the hemiptelein, it is a plant fruit having a demand for promoting growth or improving quality, and further preferably, the plant fruit is the fruit of tomatoes or watermelons. Based on the inspiration or idea of the technical solution of the present application, the use object of the plant growth regulating composition containing the scutellarein and the hemiptelein can be further extended to melons, fruits, and vegetables.

[0013] Also, the application provides the use of the plant growth regulating composition containing scutellarein and lobin in improving the yield and / or quality of crops, wherein the plant growth regulating composition containing scutellarein and lobin is applied during the growth period of the plant or before the harvest of the fruit. The application of the plant growth regulating composition containing scutellarein and lobin before the harvest of the fruit of the plant is only a common or conventional practice, but the application does not exclude the application of the plant growth regulating composition containing scutellarein and lobin after the harvest of the fruit of the plant, especially for the fruit of the plant which needs to be ripened to obtain better quality. The application does not make any special limitation on the use concentration of the plant growth regulating composition containing scutellarein and lobin, and the use concentration provided in the example part is only an example for a specific use object in a specific regional environment.

[0014] Compared with the prior art, the technical solution provided by the application has at least the following advantages:

[0015] The application provides the plant growth regulating composition containing scutellarein and lobin, and the effective components for regulating the growth of the plant and the fruit are composed of scutellarein and lobin, and the mass ratio of the scutellarein to the lobin is 1:30-13:1. It is verified by the experiment of watermelon seedlings that, under the same application concentration, the growth effect of the lobin on the plant height, fresh weight and dry weight of the watermelon seedlings is more obvious than that of the scutellarein, and the growth rates of the plant height, fresh weight and dry weight are 63.80%, 91.74% and 91.36% respectively. When the scutellarein and the lobin are used in combination within the ratio range of 1:30-13:1, the effect is significantly higher than that of the single use of the lobin or the scutellarein, and the synergistic effect is shown.

[0016] It is verified by the greenhouse experiment of tomatoes that the scutellarein has no improving effect on the quality of the tomato fruit, and even has a slight decrease (not significant decrease); the lobin has a certain improving effect on the quality of the tomato fruit, but does not significantly improve the quality. When the scutellarein and the lobin are used in combination within the ratio range of 1:30-13:1, the quality of the tomato fruit can be significantly improved. The use of the scutellarein and the lobin in combination has a yield-increasing effect on the tomato, and the yield-increasing rate reaches 35.2-38.7% within the ratio range of 1:30-13:1, which is higher than 10.3-25.0% of the lobin and 4.6-15.8% of the scutellarein, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate after the use of the scutellarein and the lobin in combination is greater than 10%, and the synergistic effect is shown. DETAILED DESCRIPTION

[0017] The technical solutions of the present application are described below in combination with examples, but the present application is not limited to the following examples. The experimental methods and detection methods described in each example are all conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified. Scopoletin is purchased from Shandong Provincial Fanyuan Chemical Co., Ltd., with a purity of 99%. Scutellarein is purchased from Xi'an Jiahe Biotechnology Co., Ltd., with a purity of 98%.

[0018] Example 1

[0019] This example provides the effect of combined use of scutellarein and scopoletin on the growth of watermelon seedlings.

[0020] A potting method test is used, and watermelon seedlings are grown outdoors. The watermelon seedlings are sprayed when they grow to 2 cotyledons and 1 true leaf. The concentration of the effective components is uniformly 20 mg / L, 100 mL of pesticide solution is poured per pot (dispersed thoroughly before spraying), and the positive control agent is scopoletin or scutellarein, and the blank control agent is water. After spraying, the seedlings are cultured under normal outdoor growth conditions. After 1 week, the growth rate of the plant height, fresh weight and dry weight of the watermelon seedlings is investigated, and the experimental results are shown in Table 1.

[0021] Growth rate (%) = [(treated group plant height, fresh weight or dry weight - blank group plant height, fresh weight or dry weight) / blank group plant height, fresh weight or dry weight] x 100%

[0022] Table 1 Growth rate of plant height, fresh weight and dry weight of watermelon seedlings

[0023]

[0024]

[0025] As shown in Table 1, under the same application concentration, the growth effect of scopoletin on the plant height, fresh weight and dry weight of watermelon seedlings is more obvious than that of scutellarein, and the growth rates of the plant height, fresh weight and dry weight are 63.80%, 91.74% and 91.36%, respectively. When scutellarein and scopoletin are used in combination in a ratio of 1:30 to 13:1, the effect is significantly higher than that of single use of scopoletin or scutellarein, showing a synergistic effect. When scutellarein and scopoletin are used in combination, the ratio changes, and the effect on the plant height, fresh weight and dry weight of watermelon seedlings also changes, which shows that although scutellarein and scopoletin are both plant growth regulators, the effects they exert or the pathways they regulate are different.

[0026] Example 2

[0027] This example provides the effect of combined use of scutellarein and scopoletin on the yield and fruit quality of tomatoes.

[0028] Tomato (Shenfen 16) seeds were soaked in warm soup, germinated, and then sown in 76-hole hole trays. When the seedlings grew to 2 true leaves, they were transplanted to a greenhouse. When applying the pesticide, a small amount of pesticide was dipped with a brush and gently applied to the just-opened female flower stalk. After the fruit ripened, 3-4 fruits with regular shape, uniform size, and no disease and insect pests were selected from the 2nd and 3rd clusters for soluble protein content determination (using Coomassie brilliant blue staining method). The positive control agent was scabrousleaf or scabiosa sinensis, and the blank control agent was water. The total yield was recorded during the harvest period, and the results are shown in Table 2. The yield increase rate in Table 2 is calculated as follows:

[0029] Actual yield increase rate E (%) = [(total yield of pesticide treatment group - total yield of blank control group) / total yield of blank control group] x 100%

[0030] Theoretical yield increase rate E0 (%) of two pesticide combinations = X + Y - XY / 100

[0031] In the formula, X represents the actual yield increase rate E of scabiosa sinensis at a dosage of P; Y represents the actual yield increase rate E of scabrousleaf at a dosage of Q, and E0 represents the theoretical yield increase rate of scabiosa sinensis and scabrousleaf combined application at a dosage of P+Q. According to the Gowing method in NY / T2061.5-2016, the combined effect type of the mixture is evaluated. E-E0>10% is synergistic effect, E-E0<-10% is antagonistic effect, and the value of E-E0 is within ±10% is additive effect.

[0032] Table 2 Effect of combined application of scabiosa sinensis and scabrousleaf on tomato yield and fruit quality

[0033]

[0034]

[0035] The soluble protein of tomato fruit treated with blank control agent was 4.9 mg / g. Soluble protein is an important osmoregulatory substance that plays an important role in maintaining cell osmotic pressure. At the same time, soluble protein not only reflects the nutritional value of fruit, but also reflects the stress resistance of plants. As shown in Table 2, scabiosa sinensis had no improvement effect on tomato fruit quality, and even slightly decreased (not significantly decreased); scabrousleaf had a certain improvement effect on tomato fruit quality, but did not significantly improve it. When scabiosa sinensis and scabrousleaf were combined, within the ratio range of 1:30-13:1, the quality of tomato fruit was significantly improved. Tomato quality is closely related to non-volatile components in the fruit. After the combined application of scabiosa sinensis and scabrousleaf, the content of certain or some components in the tomato fruit changed, affecting the quality of the tomato, resulting in improved quality of tomato fruit treated with scabiosa sinensis and scabrousleaf. However, the mechanism of how scabiosa sinensis and scabrousleaf affect tomato quality still needs further research.

[0036] Based on the statistics of total yield, it can be seen from Table 2 that the combination of scutellarein and scutellarein has yield-increasing effect on tomato, and the yield-increasing rate reaches 35.2-38.7% in the ratio range of 1:30-13:1, which is higher than that of scutellarein (10.3-25.0%) and scutellarein (4.6-15.8%), and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate after the combination of scutellarein and scutellarein is greater than 10%, which shows the synergistic effect; when the ratio of scutellarein and scutellarein is 4:2, the synergistic effect is best, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate is 23.8%.

[0037] The above-described embodiments are part of the embodiments of the present application, rather than all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents selected embodiments of the application. All other embodiments obtained by related deduction and replacement made by those skilled in the art under the condition of the concept of the present application, without making creative labor, belong to the scope of protection of the present application.

Claims

1. A plant growth regulator composition comprising ligustrazine and hemifolin, characterized in that, The active ingredients of the plant growth regulating composition consist of scutellarin and hemifolin, wherein the mass ratio of scutellarin to hemifolin is 1:30 to 13:

1.

2. The plant growth regulator composition comprising ligustrazine and hemifolin according to claim 1, characterized in that, The mass ratio of the ligustrazine and hemifolin is 1:5 to 4:

1.

3. The plant growth regulator composition comprising ligustrazine and hemifolin according to claim 2, characterized in that, The mass ratio of the ligustrazine to the hemifolin is 2:

1.

4. The use of the plant growth regulator composition comprising ligustrazine and hemifolin as described in any one of claims 1 to 3 in improving crop yield.

5. The use of the plant growth regulator composition comprising ligustrazine and hemifolin as described in any one of claims 1 to 3 in improving the quality of crop fruits.

6. The application according to claim 4, characterized in that, Used to increase the yield and / or quality of tomatoes or watermelons.

7. The application according to claim 5, characterized in that, Used to increase the yield and / or quality of tomatoes or watermelons.

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