A pesticide composition containing biogenic insecticide and its application

Abstract

The present invention belongs to the field of pesticide control technology and discloses a pesticide composition containing a biogenic insecticide and its use. The active ingredients of the pesticide composition include a compound of Formula I and a biogenic insecticide. The structure of the compound of Formula I is as follows: #imgabs0# The biogenic insecticide is either spinetoram or spinosad, and the mass ratio of the compound of Formula I to the biogenic insecticide is 1:58 to 58:1. The pesticide composition of the present invention has excellent control effects on various pests in agriculture, horticulture, and forestry, reduces the dosage of pesticides used, and slows the development of pest resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide killing technology and discloses a pesticide composition containing a biogenic pesticide and application thereof. Background Art

[0002] Biogenic pesticides are pesticides that use biological metabolites as their main insecticidal ingredients. For example, microbial pesticides are secondary metabolites produced by bacteria and fungi that are toxic to pests. They are extracted and developed into biological pesticides through artificial fermentation processes to kill pests. Such pesticides have less harm to the environment and are not easy to leave residues.

[0003] Spinetoram is a macrolide biogenic insecticide developed in recent years by Dow AgroSciences of the United States. It mainly acts on nicotinic acetylcholine receptors and γ-aminobutyric acid receptors in the insect nervous system, rendering the insect insensitive to excitatory or inhibitory signal transmission, leading to death.

[0004] Spinosad, also known as spinosad, is a macrolide biogenic compound and a secondary metabolite obtained by aerobic fermentation of soil actinomycetes. It has good insecticidal activity against a variety of lepidopteran pests and has low toxicity to natural enemies of insects and mammals. It degrades rapidly in soil and water and is safe for the environment.

[0005] The compound of formula I is a new compound independently developed by our company. This compound has a broad insecticide spectrum, low toxicity and high efficacy. The structure of the compound of formula I is shown below:

[0006]

[0007] At present, pests are still mainly controlled by chemical means, but the long-term irrational use of pesticides causes the continuous occurrence and development of pest resistance. In view of the above problems, the rational compounding of agents with different insecticide mechanisms can be selected, which can not only improve the efficacy and reduce the cost, but also be one of the important methods to overcome the development of pest resistance. Therefore, the inventors explored the combined effect of the compound of formula I and the biogenic insecticide spinosad or ethyl spinetoram on various pests, aiming to provide a theoretical basis for reducing the amount of pesticide used for pest control and improving the control effect. However, there is currently no report on the control of pests by the compound of formula I and spinosad or ethyl spinetoram. Summary of the Invention

[0008] To address the aforementioned problems in the prior art, the present invention provides a pesticide composition containing a biogenic insecticide and its use. The pesticide composition has excellent control effects on a variety of agricultural, horticultural, and forestry pests, reduces pesticide dosage, and slows the development of pest resistance.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition containing a biogenic insecticide and its application, wherein the active ingredient of the pesticide composition comprises a compound of formula I and a biogenic insecticide, and the structure of the compound of formula I is as follows: The biogenic insecticide is any one of spinosad or ethyl spinetoram, and the mass ratio of the compound of formula I to the biogenic insecticide is 1:58 to 58:1, such as 1:58, 1:55, 1:45, 1:42, 1:40, 1:36, 1:35, 1:30, 1:25, 1:20, 1:18, 1:12, 1:10, 1:9, 1:6, 1:5, 1:2, 1:1, 2:1, 3:1, 5:1, 8:1, 9:1, 10:1, 16:1, 18:1, 20:1, 25:1, 32:1, 30:1, 35:1, 36:1, 40:1, 42:1, 45:1, 48:1, 50:1, 58:1, or any value between the above values;

[0010] Furthermore, the mass ratio of the compound of formula I to the biogenic insecticide in the pesticide composition is 1:55 to 45:1, such as 1:55, 1:45, 1:42, 1:40, 1:36, 1:35, 1:30, 1:25, 1:20, 1:18, 1:12, 1:10, 1:9, 1:6, 1:5, 1:2, 1:1, 2:1, 3:1, 5:1, 8:1, 9:1, 10:1, 16:1, 18:1, 20:1, 25:1, 32:1, 30:1, 35:1, 36:1, 40:1, 42:1, 45:1, or any value in between;

[0011] Furthermore, the biogenic insecticide is spinetoram, and the mass ratio of the compound of formula I to spinetoram is 1:45 to 32:1, such as 1:45, 1:42, 1:36, 1:35, 1:30, 1:20, 1:12, 1:10, 1:6, 1:5, 1:2, 1:1, 2:1, 5:1, 8:1, 10:1, 16:1, 20:1, 30:1, 32:1, or any value between the above values;

[0012] Furthermore, the biogenic insecticide is spinetoram, and the mass ratio of the compound of formula I to spinetoram is 1:42 to 32:1, such as 1:42, 1:36, 1:35, 1:30, 1:20, 1:12, 1:10, 1:6, 1:5, 1:2, 1:1, 2:1, 5:1, 8:1, 10:1, 16:1, 20:1, 30:1, 32:1, or any value between the above values;

[0013] Furthermore, when the pesticide composition is used to control Plutella xylostella, the mass ratio of the compound of formula I to spinetoram is 1:42 to 32:1, or any value between the above values, preferably 1:36 to 16:1, more preferably 1:12 to 16:1;

[0014] When the pesticide composition is used to control rice leaf roller, the mass ratio of the compound of formula I to spinetoram is 1:30 to 40:1, preferably 1:20 to 30:1, and more preferably 1:10 to 30:1;

[0015] When the pesticide composition is used to control western flower thrips, the mass ratio of the compound of formula I to spinetoram is 1:45 to 20:1, or any value between the above values, preferably 1:35 to 10:1, more preferably 1:20 to 5:1;

[0016] Further, the biogenic insecticide is spinosad, and the mass ratio of the compound of formula I to spinosad is 1:55 to 42:1, or any value between the above values, such as 1:55, 1:45, 1:42, 1:40, 1:36, 1:35, 1:30, 1:25, 1:20, 1:18, 1:10, 1:9, 1:5, 1:2, 1:1, 2:1, 3:1, 5:1, 9:1, 10:1, 18:1, 20:1, 25:1, 30:1, 35:1, 36:1, 40:1, 42:1, or any value between the above values;

[0017] Furthermore, the biogenic insecticide is spinosad, and the mass ratio of the compound of formula I to spinosad is 1:42 to 42:1, such as 1:42, 1:40, 1:36, 1:35, 1:30, 1:25, 1:20, 1:18, 1:10, 1:9, 1:5, 1:2, 1:1, 2:1, 3:1, 5:1, 9:1, 10:1, 18:1, 20:1, 25:1, 30:1, 35:1, 36:1, 40:1, 42:1, or any value in between;

[0018] Furthermore, when the pesticide composition is used to control Plutella xylostella, the mass ratio of the compound of formula I to spinosad is 1:55 to 25:1, or any value between the above values, preferably 1:45 to 10:1, more preferably 1:35 to 5:1;

[0019] When the pesticide composition is used to control rice leaf roller, the mass ratio of the compound of formula I to spinosad is 1:42 to 42:1, or any value between the above values, preferably 1:36 to 36:1, more preferably 1:18 to 18:1;

[0020] When the pesticide composition is used to control western flower thrips, the mass ratio of the compound of formula I to spinosad is 1:30 to 30:1, or any value between the above values, preferably 1:20 to 20:1, and more preferably 1:10 to 10:1;

[0021] Furthermore, the total weight of the pesticide composition is 100 wt%, and the compound of formula I and the biogenic insecticide account for 0.01% to 80% of the total weight of the pesticide composition;

[0022] Furthermore, the pesticide composition contains, in addition to the active ingredient, auxiliary ingredients permitted in pesticides, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

[0023] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0024] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0025] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0026] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0027] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0028] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0029] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0030] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0031] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0032] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0033] Synergists are selected from synergist, piperonyl butoxide; and / or

[0034] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0035] Furthermore, the pesticide composition is prepared into a pesticide formulation, which is a solid formulation or a liquid formulation;

[0036] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0037] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0038] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder; the liquid preparation is a suspension, a microemulsion, a dispersible oil suspension or an aqueous emulsion;

[0039] The present invention also discloses the use of the above pesticide composition for preventing and controlling agricultural, horticultural or forestry pests.

[0040] Furthermore, the pests are lepidopteran pests, thysanoptera pests or coleoptera pests;

[0041] Furthermore, the Lepidoptera includes, but is not limited to, Plutellaxylostella, diamondback moth, Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (snoctuids), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (soybean looper), Rachiplusia nu, Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides), Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp. (snooper moths), Spodoptera exigua (beet armyworm), Spodoptera fugiperda (fall armyworm), Adoxophyes spp., Adoxophyes orana, Agrotis spp.(cutworm), Agrotis ipsilon (black cutworm), Alabama argillacea, Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anarsia lineatella (peach twig borer), Anomissa bulifera (jute looper), Anticarsia gemma talis (velvet bean pelleter), Archips argyrospila (fruittree leafroller), Archips rosana (rose leaf roller), Argyrotaenia spp. (tortricidmoths), Argyrotaenia citrana, Autographa gamma, Bonagota cranaodes, Borbo cinnara), Caloptilia spp. (leaf miners), Capuareticulana, Carposina niponensis (peach fruit moth), Chilospp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded leafroller), Chrysodeixis spp., Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus, Crambus spp.) (Sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella, Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester cornborer), Earias spp. (cotton bollworms), Earias spp. (Egyptian bollworms). insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopophaaurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobbaco moth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella, Euxoa auxiliaris (army cutworm), Feltia spp., Gortyna spp.(stemborers), Grapholita molesta (oriental fruit moth), Hedylepta indicate, bean leaf webber, Helicoverpas pp. (spotworms), Helicoverpa paarmigera, Helicoverpa zea, Heliothis spp. (spotworms), Heliothis virescens, tobacco budworm, Hellula undalis (cabbage webworm), Indarbela spp., root borers, Keiferia lycopersicella, (tomato pinworm), Leucinodes orbonalis (eggplant fruit borer). borer), Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth), Loxagrotis spp. (southworm), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.(tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis, Metisa plana, Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis (small tomato borer), Operophthera brumata (winter moth), Ostrinia nubilalis (European corn borer), Pandemis cerasana, common currant tortrix, Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma species ( spp. (cutworm), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp. (leafminers), Pieris rapae (imported cabbageworm), Plathypena scabra, Plodia interpunctella (Indian mealmoth), Spodoptera oridania (southern armyworm), Synanthedon spp.) (root borers), Thecla basilides, Thermisiagemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (rape worm), Tuta absoluta, Yponomeuta spp., Zeuzera coffeae (red branch borer), and Zeuzera pyrina (leopard moth).

[0042] The Thysanoptera pests include, but are not limited to, Frankliniella occidentalis, Thrips spp., Scirtothrips dorsalis, Stenchaetothrips biformis, Frankliniella intonsta, Thrips palmi, Anaphothrips obscurus, Neohydatothrips samayunkur, Dendrothrips minowai, Haplothrips aculeatus, Frankliniella tenuicornis, and Thrips hawaiiensis.

[0043] The coleopteran pests include, but are not limited to, Acanthoscelides spp. (weevils),

[0044] Acanthos celides obtectus (common pea weevil), Agrilus planipennis (flowered ash borer), wireworm species

[0045] Agriotes spp. (wireworms), Anoplophora glabripennis (Asian long-horned beetle), Anthonomus spp. (Cumicidae), Anthonomus grandis (cotton bollworm), Aphidius spp., Apion spp. (weevils), Apogonia spp. (grubs), Atacnius sprctulus (black velvet beetle), Atomaria linearis (pygmy mangold beetle), Aulacophores spp., Bothynoderes punctiventris (beetroot weevil), Bruchus spp. (weevils), Bruchus pisorum (pea weevil), Cacoesia spp., Callosobruchus maculatus (southern cowpea weevil), Carpophilus hemipteras (dried fruit beetle), Cassida vittata, Ccrostcrna spp., Ccrotoma spp. (chrysomcides), Cerotoma trifur cata (bean leaf beetle), Ceutorhynchus spp. (weevils), Ceutorhynchus assimilis (cabbage seedpod weevil), Ceutorhynchus napi (cabbage curculio), Chaetocnema spp.) (Trichomonas), Colaspis spp.(earth beetle), Conoderus scalaris, Conoderus stigmosus, Conotrachelus nenuphar (plum cone weevil), Cotinus nitidis (Green June beetle), Criocerisas paragi (asparagi beetle), Cryptolestes ferrugincus (rusty grain beetle), Cryptolestes pusillus (grain beetle), Cryptolestes turcicus (Turkish grain beetle), Ctenicera spp. (nematodes), Curculio spp. (weevils), Cyclocephala spp. (grubs), Cylindrocpturus adspersus (sunflower stem weevil), Deporaus marginatus (mangoleaf-cutting weevil), Dermestes lardarius (ham weevil), Dermestes maculates (white-bellied weevil), Diabrotica spp. (leaf beetles), Epilachna varivcstis (Mexican bean beetle), Raustinus cubae (stem borer), Hylobius pales (pales weevil), Hypera spp. (weevils), Hypera postica (alfalfa weevil), Hyperdoes spp. (leaves beetles), spp.) (Hyperodes weevil), Hypothenemus hampei (coffee berry beetle), Ips spp.) (engravers), Lasioderma serricorne (tobacco beetle), Leptinotarsa decemlineata (Colorado potato beetle), Liogenys fuscus, Liogenys suturalis, Lissorhoptrus oryzophilus (rice water weevil), Lyctus spp. (powder post beetles), Maecolaspis joliveti, Megascelis spp., Melanotus communis, Meligethes spp., Meligethes aeneus (blossom beetle), Melolontha melolontha (common European beetle), Oberea brevis, Oberea linearis, Oryctes rhinoceros (date palm beetle), Oryzaephilus mercator (merchant grain beetle), Oryzaephilus surinamensis (sawtooth grain beetle), Otiorhynchus spp. (weevils), Oulema melanopus (cereal leaf beetle), Oulema oryzae, Pantomorus spp. (weevils), Phyllophaga spp. (May / June beetles), Phyllophaga cuyabana, Phyllotreta spp. (yellow flea beetles), spp.) (Trichomonas), Phynchites spp.), Popillia japonica (Japanese beetle), Prostephanus truncates (larger grain borer), Rhizopertha dominica (lesser grain borer), Rhizotrogus spp. (European chafer), Rhynchophorus spp. (weevils), Scolytus spp. (wood moths), Shenophorus spp. (grain weevils), Sitona lincatus (pea leaf weevil), Sitophilus spp. (grain weevils), Sitophilus granaries (granary weevil), Sitophilus oryzae (rice weevil) weevil), Stegobium paniceum (drugstore beetle), Tribolium spp. (face weevil), Tribolium castaneum (red flour beetle), Tribolium confusum (confused flour beetle), Trogoderma variabile (warehouse beetle), and Zabrus tenebioides.

[0046] In particular, the pesticide composition of the present invention has excellent control effects on diamondback moth, beet armyworm, rice leaf folder, thrips and yellow flea beetle.

[0047] Furthermore, the pesticide composition or its formulation is applied to the pests to be controlled or the medium in which they grow.

[0048] In order to obtain the desired insecticidal effect, the dosage of the pesticide composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0049] The beneficial effects of the present invention are as follows:

[0050] 1) The pesticide composition of the present invention rationally combines compounds with different mechanisms of action, and has a significant synergistic effect on pests at a certain mass ratio;

[0051] 2) The pesticide composition of the present invention has excellent control effects on Lepidoptera, Thysanoptera and Coleoptera pests in agriculture, horticulture and forestry;

[0052] 3) The pesticide composition of the present invention reduces the amount of pesticide used, has less impact on the environment, and slows down the occurrence of pesticide resistance in pests. DETAILED DESCRIPTION

[0053] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0054] Preparation example:

[0055] Preparation Example 1: 42% Formula I Compound·Spinetoram Water Dispersible Granules (1:2)

[0056] Formula composition: 14% compound of formula I, 28% spinetoram, 12% naphthalenesulfonate formaldehyde condensate, 6% sodium salt of polycarboxylate, 2% sodium lauryl sulfate, 12% ammonium sulfate, and starch makes up the balance;

[0057] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then the mixture is kneaded, granulated, dried, and sieved to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0058] Preparation Example 2: 30% Formula I compound·Spinetoram wettable powder (1:1)

[0059] Formula composition: 15% compound of formula I, 15% spinetoram, 5% sodium lignin sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 3% pulverized powder BX, 8% white carbon black, and kaolin to make up the balance;

[0060] Preparation method: According to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0061] Preparation Example 3: 36% Formula I Compound·Spinetoram Suspension Concentrate (1:5)

[0062] Formula composition: 6% compound of formula I, 30% ethyl spinosad, 1% isomeric tridecanol polyoxyethylene ether, 3% ethylene glycol oxyethylene polyoxypropylene ether, 2% polyoxyethylene sorbitan monooleate, 3% tristyrylphenol ethoxylate phosphate, 1% sodium salt of polycarboxylate, 1% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% glycerol, 0.5% silicone oil, and deionized water to make up the balance;

[0063] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0064] Preparation Example 4: 4% Formula I Compound·Spinetoram Microemulsion (3:1)

[0065] Formula composition: 3% compound of formula I, 1% ethyl spinosad, 30% cyclohexanone, 13% tristyrylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 3% fatty alcohol polyoxyethylene ether sodium sulfate, 1% fatty alcohol polyoxyethylene ether phosphate, 0.05% silicone defoamer, and deionized water to make up the balance;

[0066] Preparation method: According to the formula ratio, the active ingredient is completely dissolved in the solvent, and then emulsified to prepare the oil phase. The dispersant, deionized water, etc. are stirred evenly to prepare the water phase; the oil phase is added to the water phase and stirred evenly, and high-speed shearing is performed until the particle size meets the requirements. After adding the defoaming agent, stir evenly to obtain the microemulsion product.

[0067] Preparation Example 5: 4.5% Formula I Compound·Spinetoram Emulsion (8:1)

[0068] Formula composition: 4% compound of formula I, 0.5% ethyl spinosad, 30% cyclohexanone, 5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% alkylphenol polyoxyethylene ether phosphate, 5% ethylene glycol, 0.05% silicone defoamer, and deionized water to make up the balance;

[0069] Preparation method: After completely dissolving the active ingredient in the solvent according to the formula ratio, add the emulsifier to form the oil phase; stir the dispersant, antifreeze, deionized water, etc. to form the water phase; add the oil phase to the water phase and stir evenly, shear at high speed until the particle size meets the requirements, add the defoaming agent and stir evenly to obtain the water emulsion product.

[0070] Preparation Example 6: 20% Formula I Compound·Spinosad Water Dispersible Granules (2:3)

[0071] Formula composition: 8% compound of formula I, 12% spinosad, 7% sodium lignin sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% flaking powder BX, 1.5% sodium dodecylbenzene sulfonate, 12% ammonium sulfate, and kaolin makes up the balance;

[0072] Preparation method: Same as Preparation Example 1.

[0073] Preparation Example 7: 24% Formula I compound·Spinosad wettable powder (3:1)

[0074] Formula composition: 18% compound of formula I, 6% spinosad, 6% tea saponin, 5% sodium lignin sulfonate, 2% naphthalene sulfonate, 2% sodium lauryl sulfate, 8% attapulgite, and kaolin makes up the balance;

[0075] Preparation method: Same as Preparation Example 2.

[0076] Preparation Example 8: 18% Formula I Compound·Spinosad Suspension Concentrate (1:8)

[0077] Formula composition: 2% compound of formula I, 16% spinosad, 2% glycerol fatty acid ester polyoxyethylene ether, 1% naphthalenesulfonate formaldehyde condensate, 3% tristyrylphenol ethoxylate phosphate, 2% alkylphenol polyoxyethylene ether, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0078] Preparation method: Same as Preparation Example 3.

[0079] Preparation Example 9: 3% Formula I Compound·Spinosad Microemulsion (5:1)

[0080] Formula composition: 2.5% compound of formula I, 0.5% spinosad, 12% xylene, 25% cyclohexanone, 15% phenylethylphenol polyoxyethylene polyoxypropylene ether, 4% EO-PO block copolymer, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 5% ethylene glycol, 0.05% silicone defoamer, and deionized water to make up the balance;

[0081] Preparation method: same as Preparation Example 4.

[0082] Preparation Example 10: 2.2% Formula I Compound·Spinosad Emulsion in Water (10:1)

[0083] Formula composition: 2% compound of formula I, 0.2% spinosad, 5% alkylaryl polyoxyethylene ether polyoxypropylene ether, 2% polyoxyethylene sorbitan monooleate, 10% cyclohexanone, 0.2% xanthan gum, 5% propylene glycol, 1% urea, 0.5% potassium benzoate, 0.05% organosilicon defoaming agent, and deionized water to make up the balance;

[0084] Preparation method: Same as Preparation Example 5.

[0085] Indoor biological activity assay

[0086] Example 1: Indoor bioactivity test on the lepidopteran pest Plutella xylostella

[0087] Test basis: The test refers to NY / T-1154.14-2008 "Guidelines for Indoor Biological Testing of Pesticides - Insecticides Part 14: Leaf Dipping Method".

[0088] Test target: 3rd instar larvae of Plutella xylostella.

[0089] Test agents: compound of formula I, spinetoram, and spinosad technical.

[0090] Test Method: Based on the preliminary test results, the above raw drugs were dissolved in a suitable solvent and then diluted with a 0.1% Tween 80 solution to a suitable concentration gradient. A treatment without the drug (containing all organic solvents and emulsifiers) served as a blank control. Clean, untreated cabbage leaves were selected and leaf discs were punched out using a 2-cm diameter hole punch. The leaf discs were immersed in the prepared drug solution for 10 seconds, removed, dried at room temperature, and placed in a Petri dish lined with moisturizing filter paper. Ten test insects were seeded into each dish, and four replicates were used for each treatment.

[0091] Experimental investigation: 48 hours after treatment, the number of dead larvae in each treatment was counted. The insect bodies were lightly touched with a small brush or tweezers. Individuals that could not coordinate movement or whose bodies differed greatly from those of the control were considered dead.

[0092] Calculation method:

[0093] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0094]

[0095] Where:

[0096] P——mortality rate, in percentage (%);

[0097] K——indicates the number of dead insects, the unit is head;

[0098] N——represents the total number of insects processed, in heads.

[0099]

[0100] Where:

[0101] P1——adjusted mortality rate, in percentage (%);

[0102] P t ——Treatment mortality rate, expressed in percentage (%);

[0103] P0 - blank control mortality rate, in percentage (%).

[0104] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0105] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0106] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0107]

[0108] Where:

[0109] ATI - measured toxicity index of mixture;

[0110] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0111] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0112] TTI=TI A ×P A +TI B ×P B

[0113] Where:

[0114] TTI – Theoretical Toxicity Index of Mixtures;

[0115] TI A ——Agent toxicity index;

[0116] P A ——The percentage of agent A in the mixture, in percentage (%);

[0117] TI B ——Toxicity index of agent B;

[0118] P B ——The percentage of agent B in the mixture, in percentage (%).

[0119]

[0120] Where:

[0121] CTC – Co-toxicity coefficient;

[0122] ATI - measured toxicity index of mixture;

[0123] TTI - Theoretical Toxicity Index of Mixture.

[0124] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0125] The indoor test results are shown in the table below:

[0126] Table 1 Indoor biological activity test results of compound I combined with spinetoram against Plutella xylostella

[0127]

[0128]

[0129] Table 2 Indoor biological activity test results of compound I combined with spinosad against Plutella xylostella

[0130]

[0131] From the test results in Table 1 and Table 2, it can be seen that the compound of formula I combined with spinetoram or spinosad showed good activity against Plutella xylostella.

[0132] The mass ratio of the compound of formula I and ethyl spinetoram is 1:42-32:1, and the co-toxicity coefficient to the diamondback moth is greater than 120, showing a synergistic effect; the mass ratio of the two is 1:36-16:1, and the co-toxicity coefficient to the diamondback moth is greater than 140, showing a significant synergistic effect; and the mass ratio of the two is 1:55 and 48:1, and the co-toxicity coefficient is less than 120, showing an additive effect.

[0133] The mass ratio of the compound of formula I to spinosad is 1:55 to 25:1, and the co-toxicity coefficient to the diamondback moth is greater than 120, showing a synergistic effect; the mass ratio of the two is 1:45 to 10:1, and the co-toxicity coefficient to the diamondback moth is greater than 140, with a significant synergistic effect; and the mass ratio of the two is 35:1, which shows an additive effect.

[0134] Example 2: Indoor bioactivity test on lepidopteran pest Cnaphalocrocis medinalis

[0135] Test basis: The test refers to NY / T-1154.14-2008 "Guidelines for Indoor Biological Testing of Pesticides - Insecticides Part 14: Leaf Dipping Method".

[0136] Test target: 3rd instar larvae of rice leaf folder.

[0137] Test agents: compound of formula I, spinetoram, and spinosad technical.

[0138] Preparation of test agents: After dissolving the above agents in appropriate solvents, dilute them to appropriate concentrations with water containing 0.1% Tween-80, and use a treatment without agents (containing all organic solvents and emulsifiers) as a blank control.

[0139] Test Method: A rice leaf immersion method was used. Fresh, tender rice leaves were immersed in each treatment solution for 20 seconds. After removal, they were air-dried at room temperature. The lower portion of the leaves were wrapped with soaked cotton wool and placed in a culture cup, with six leaves per cup. Ten third-instar larvae of the rice leaf roller were then inoculated and sealed with gauze. Each treatment was replicated four times. After treatment, the test insects were placed in an artificial climate chamber at (26 ± 1)°C with a photoperiod of L:D = 16 h:8 h.

[0140] Experimental investigation: 72 hours after treatment, the number of dead insects in each treatment was investigated. The criterion for death was that the insect body could not coordinate movement when the insect body was lightly touched with a brush.

[0141] Calculation method:

[0142] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0143]

[0144] Where:

[0145] P——mortality rate, in percentage (%);

[0146] K——indicates the number of dead insects, the unit is head;

[0147] N——represents the total number of insects processed, in heads.

[0148]

[0149] Where:

[0150] P1——adjusted mortality rate, in percentage (%);

[0151] P t ——Treatment mortality rate, expressed in percentage (%);

[0152] P0 - blank control mortality rate, in percentage (%).

[0153] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0154] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0155] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0156]

[0157] Where:

[0158] ATI - measured toxicity index of mixture;

[0159] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0160] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0161] TTI=TI A ×P A +TI B ×P B

[0162] Where:

[0163] TTI – Theoretical Toxicity Index of Mixtures;

[0164] TI A ——Agent toxicity index;

[0165] P A ——The percentage of agent A in the mixture, in percentage (%);

[0166] TI B ——Toxicity index of agent B;

[0167] P B ——The percentage of agent B in the mixture, in percentage (%).

[0168]

[0169] Where:

[0170] CTC – Co-toxicity coefficient;

[0171] ATI - measured toxicity index of mixture;

[0172] TTI - Theoretical Toxicity Index of Mixture.

[0173] A co-toxicity coefficient (CTC) of ≥120 indicates a synergistic effect; a CTC of ≤80 indicates an antagonistic effect; and a CTC of 80 < CTC <120 indicates an additive effect. The results of the laboratory test are shown in the table below:

[0174] Table 3 Indoor biological activity test results of the compound of formula I and spinetoram against rice leaf roller

[0175]

[0176] Table 4 Indoor biological activity test results of the compound of formula I combined with spinosad against rice leaf roller

[0177]

[0178]

[0179] It can be seen from the test results in Table 3-4 that the compound of formula I and spinetoram or spinosad in a suitable mass ratio show a good control effect on rice leaf folder.

[0180] When the mass ratio of the compound of formula I to ethyl spinetoram is 1:30-40:1, the co-toxicity coefficient to the rice leaf folder is greater than 120, showing a synergistic effect; when the mass ratio of the two is 1:20-30:1, the co-toxicity coefficient is greater than 140, and the synergistic effect is obvious; when the mass ratio of the two is 1:5-20:1, the co-toxicity coefficient is greater than 170, and the synergistic effect is obvious; and when the mass ratio of the two is 50:1, the co-toxicity coefficient to the rice leaf folder is less than 120, showing an additive effect.

[0181] When the mass ratio of the compound of formula I to spinosad is 1:42 to 42:1, the co-toxicity coefficient to the rice leaf folder is greater than 120, showing a synergistic effect; when the mass ratio of the compound of formula I to spinosad is 1:36 to 18:1, the co-toxicity coefficient is greater than 140, and the synergistic effect is significant; when the mass ratio of the two is 1:18 to 9:1, the co-toxicity coefficient is greater than 160, and the synergistic effect is significant; and when the mass ratio of the two is 1:58 and 58:1, the co-toxicity coefficient to the rice leaf folder is less than 120, showing an additive effect.

[0182] Example 3: Indoor biological activity test on Thysanoptera pests

[0183] Test target: Adult western flower thrips.

[0184] Test agents: compound of formula I, spinetoram, and spinosad technical.

[0185] Preparation of drugs: After dissolving the above drugs in appropriate solvents, dilute them to appropriate concentrations with water containing 0.1% Tween-80. Use the treatment without drugs (containing all organic solvents and emulsifiers) as a blank control.

[0186] Test Method: The test used the leaf tube film method. Each 1.5 mL centrifuge tube was filled with each of the above chemicals. After 4 hours, the solution was discarded and the tubes were placed on a bench to dry. A 2-3 mm hole was punched in the bottom of the tube using a fine needle. Each tube served as a replicate, and each concentration was repeated four times. A tube immersed in water served as a control. Fresh cabbage leaves were punched into 1 cm diameter circular discs using a hole punch. The leaves were immersed in each concentration of chemical solution for 10 seconds. A blank control was also immersed in water. The immersed leaves were removed, dried, and placed in centrifuge tubes containing the corresponding chemical concentrations, with one leaf per tube. Test insects were placed in the centrifuge tubes using a sucker, with 10 test insects per replicate. The tubes were capped and the holes in the bottom of the tubes sealed with parafilm. The treated insects were placed in an artificial climate chamber at 25°C with a light cycle of 16:8 hours.

[0187] Experimental investigation: The mortality rate was checked after 48 hours. The criterion for judging death was that if the insect crawled no more than one insect length when lightly touched with the tip of a brush, it was considered dead.

[0188] Calculation method:

[0189] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0190]

[0191] Where:

[0192] P——mortality rate, in percentage (%);

[0193] K——indicates the number of dead insects, the unit is head;

[0194] N——represents the total number of insects processed, in heads.

[0195]

[0196] Where:

[0197] P1——adjusted mortality rate, in percentage (%);

[0198] P t ——Treatment mortality rate, expressed in percentage (%);

[0199] P0 - blank control mortality rate, in percentage (%).

[0200] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0201] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50The activity of the test agent on the biological test material is evaluated by the value.

[0202] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0203]

[0204] Where:

[0205] ATI - measured toxicity index of mixture;

[0206] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0207] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0208] TTI=TI A ×P A +TI B ×P B

[0209] Where:

[0210] TTI – Theoretical Toxicity Index of Mixtures;

[0211] TI A ——Agent toxicity index;

[0212] P A ——The percentage of agent A in the mixture, in percentage (%);

[0213] TI B ——Toxicity index of agent B;

[0214] P B ——The percentage of agent B in the mixture, in percentage (%).

[0215]

[0216] Where:

[0217] CTC – Co-toxicity coefficient;

[0218] ATI - measured toxicity index of mixture;

[0219] TTI - Theoretical Toxicity Index of Mixture.

[0220] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0221] The indoor test results are shown in the table below:

[0222] Table 5 Indoor bioactivity test results of compound I combined with spinetoram against western flower thrips

[0223]

[0224] Table 6 Indoor biological activity test results of compound I combined with spinosad against western flower thrips

[0225]

[0226] From the test results in the above table (see Tables 5-6), it can be seen that compounding the compound of formula I with spinetoram or spinosad in an appropriate mass ratio has a certain synergistic effect on western flower thrips.

[0227] When the compound of formula I is compounded with spinetoram at a mass ratio of 1:45 to 20:1, the co-toxicity coefficient is greater than 120, and a synergistic effect is exhibited against western flower thrips; when the mass ratio of the compound of formula I to spinetoram is 1:35 to 10:1, the co-toxicity coefficient is greater than 130, and a significant synergistic effect is exhibited against western flower thrips; when the mass ratio of the compound of formula I to spinetoram is 1:20 to 5:1, the co-toxicity coefficient is greater than 160, and a significant synergistic effect is exhibited against western flower thrips.

[0228] When the compound of formula I and spinosad are combined at a mass ratio of 1:30 to 30:1, the co-toxicity coefficient is greater than 120, and a synergistic effect is shown on western flower thrips; when the mass ratio of the compound of formula I and spinosad is 1:30 to 20:1, the co-toxicity coefficient is greater than 130, and a significant synergistic effect is shown; when the mass ratio of the compound of formula I and spinosad is 1:20 to 20, the co-toxicity coefficient is greater than 140, and a significant synergistic effect is shown.

[0229] Field efficacy trials

[0230] Example 4: Field efficacy test for controlling diamondback moth

[0231] Test basis: The test refers to GB / T 17980.13-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling lepidopteran larvae of cruciferous vegetables".

[0232] Test location: The test site is located in Daying Village, Dahuangzhuang Town, Huailai County, Zhangjiakou City, Hebei Province. The previous crop in the test site was tomato, and the soil fertility was above average. Cabbage was cultivated on flat beds. According to conventional production management, the varieties planted in each plot, sowing period, transplanting period, planting density, etc. were consistent, and the drainage and irrigation management were also consistent, and the growth was basically the same.

[0233] Experimental crops: Cabbage (Green Treasure).

[0234] Target of prevention and control: Diamondback moth.

[0235] Experimental design: The experiment was conducted with 8 treatments, 4 replicates per treatment, and 32 plots in total. Each plot was 20 m2 in size. 2 All plots were arranged in randomized blocks. Application began on August 21, 2023, during the peak infestation period of the second and third instar diamondback moth. Application was performed using a MATABI Super Green 16 backpack sprayer. The spray was diluted with water and sprayed on the entire cabbage plant.

[0236] Efficacy survey: Survey the base insect population before application, and survey again 3 and 14 days after application to determine the number of surviving insects for each treatment. Sampling is done at five diagonal points, with two cabbage plants surveyed at each point. The number of live insects on the entire cabbage plant is counted. Control efficacy is calculated based on this data.

[0237] Calculation method of prevention effect:

[0238]

[0239] Field efficacy test results:

[0240] Table 7 Results of field efficacy test on Plutella xylostella

[0241]

[0242]

[0243] Impact on cabbage and other organisms: During the field trial, no pesticide damage occurred in any treatment group, and no adverse effects were found on the surrounding environment and other beneficial organisms in the test area, indicating that the test agents were safe for cabbage growth at the doses provided.

[0244] Three days after application of the different agents, the results are shown in Table 7. The control efficacy of the 4% Formula I compound·spinetosad microemulsion (3:1), 18% Formula I compound·spinetosad suspension concentrate (1:8), 36% Formula I compound·spinetosad suspension concentrate (1:5), and 20% Formula I compound·spinetosad water dispersible granules (2:3) were 95.29%, 92.84%, 96.33%, and 93.87%, respectively, demonstrating the rapid effectiveness of the pesticide compositions of the present invention. Fourteen days after application, the 18% Formula I compound·spinetosad suspension concentrate (1:8) achieved the highest control efficacy of 98.94%. These four combination treatments exhibited superior long-lasting efficacy compared to the single-agent control.

[0245] Example 5: Field efficacy test for controlling rice leaf roller

[0246] Test basis: The test refers to GB / T 17980.2-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for control of rice leaf folders".

[0247] Site Overview: The trial was conducted in the rice paddies of Heping Village, Tongcheng City, Anhui Province. This region practices year-round rice-oil rotation, and the rice leaf roller (Cnaphalocrocis medinalis) is a frequent infestation. The soil fertility of the trial site was moderate, and rice was growing well. Cultivation conditions and fertilizer and water management were consistent across all plots.

[0248] Test subjects and test crops: The test subject was rice leaf roller, and the test rice variety was Tailiangyou 217.

[0249] Experimental design: The experiment was conducted with 8 treatments, 4 replicates per treatment, and 32 plots with an area of 20 m2. 2 The plots are arranged in random blocks, and small ridges are built between the plots to prevent water from flowing through.

[0250] Application time: The experiment was carried out on August 10, 2023, at the rice heading stage, using a JACTO HD400 backpack sprayer for one application.

[0251] Experimental investigation: The number of curled leaves in each treatment was investigated 7 and 14 days after application. The investigation used a five-point sampling method, with five rice clumps surveyed at each point. The total number of leaves surveyed and the number of curled leaves were recorded, and the curl rate and control effect were calculated.

[0252] Calculation method of drug efficacy:

[0253]

[0254] Safety investigation: On the 3rd, 7th and 14th day after application, observe whether the rice in each pesticide treatment area has any pesticide damage.

[0255] The results of the field efficacy test are shown below:

[0256] Table 8 Field efficacy test results for controlling rice leaf roller

[0257]

[0258] Safety survey results: Observations on days 3, 7, and 14 after application showed that rice in all treatment areas grew normally, with no noticeable abnormalities such as discoloration, deformities, or yellowing of leaf tips. Plant height and leaf color were similar to those in the water control area, demonstrating that all tested agents were safe for rice growth.

[0259] Seven days after application, 36% Formula I compound·spinetosad suspension concentrate (1:5), 27% Formula I compound·spinetosad emulsion (8:1), 24% Formula I compound·spinetosad wettable powder (3:1), and 18% Formula I compound·spinetosad suspension concentrate (1:8) all showed good control effects against rice leaf rollers. The group treated with 4.5% Formula I compound·spinetosad emulsion (8:1) had the lowest heart rate of 0.58% and the best control efficacy of 94.39%. Fourteen days after application, the combined treatment group showed the best control efficacy, reaching over 92%, both exceeding the conventional single-dose control.

[0260] Example 6: Field efficacy test for controlling beet armyworm

[0261] Test basis: The test refers to GB / T 17980.13-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling lepidopteran larvae of cruciferous vegetables".

[0262] The trial was conducted in a cabbage field in Zhangjiawan Village, Duchang Town, Duchang County, Jiujiang City, Jiangxi Province. The soil was sandy loam with above-average fertility. No other pesticides were used for pest control during the trial. Cultivation conditions were consistent across all plots, in line with local scientific agricultural practices.

[0263] Test crops: Chinese cabbage (early maturing No. 5).

[0264] Test target: Beet armyworm.

[0265] Experimental design: The experiment set up 6 treatments, including 5 chemical treatments and 1 water control. Each treatment was repeated 4 times, and the plot area was 20m 2 The experiment was conducted using a randomized block design. The pesticide was applied once on July 14, 2023, on a cloudy day with a temperature of 26-35°C. A Linong HD-400 backpack sprayer was used for whole-plant spraying.

[0266] Pesticide application survey: During the survey, 10 plants were selected from each plot to investigate the number of larvae of each instar on the whole plant. A base survey was conducted before applying the pesticide. The number of live insects in each treatment was investigated 3 days and 10 days after the application of the pesticide, and the control effect was calculated.

[0267] Calculation method of drug efficacy:

[0268]

[0269] Field efficacy test results:

[0270] Table 9 Results of field efficacy test on control of beet armyworm

[0271]

[0272] As can be seen from Table 9, the control effect of each agent was 83.07% to 96.52% when investigated 3 days after application, and the control effect of each agent was 73.97% to 99.25% when investigated 10 days after application.

[0273] Example 7: Field efficacy test for controlling thrips

[0274] Test basis: The test refers to NY / T 1464.6-2007 "Guidelines for Field Efficacy Tests of Pesticides Part 6: Insecticides for Control of Vegetable Thrips".

[0275] Experimental location: The experiment was conducted in a pepper greenhouse in Taiping Village, Shanghe County, Jinan City, Shandong Province. The soil fertility of the experimental site was medium, which was in line with local scientific agricultural practices.

[0276] Test target: Thrips (mixed populations of Thrips palmi and Thrips occidentalis).

[0277] Experimental crops: pepper.

[0278] Experimental design: The experiment set up 5 pesticide treatments, with spraying water as a blank control, and each treatment was repeated 4 times. No other pesticides were used before spraying the test pesticides, and the plants in all test plots grew uniformly. The plots were arranged randomly, with protection rows added around the plots, and each plot was 20m2. 2 .

[0279] Test method: The test was conducted on May 11, 2023. The pesticide was applied once. The application equipment was a MATABI Super Green 16 backpack sprayer with a double nozzle. The plants were evenly sprayed with the pesticide.

[0280] Survey Methods: Survey the base population before application and the number of surviving insects 3 and 7 days after application. Sampling was conducted at five locations per plot, with four pepper plants fixed at each location. Five flowers were randomly selected from each pepper plant, and the number of thrips present was recorded to calculate control efficacy.

[0281] Calculation method of drug efficacy:

[0282]

[0283] Field efficacy test results:

[0284] Table 10 Results of field efficacy test on thrips

[0285]

[0286] Safety investigation: During the field test, no pesticide damage occurred in any treatment group, and no adverse effects were found on the surrounding environment and other beneficial organisms in the test area, indicating that the test agents were safe for pepper growth at the doses provided.

[0287] The results (see Table 10) showed significant differences in efficacy between the different agents. Three and seven days after application, the 20% Formula I compound·spinosad water dispersible granules (2:3) and the 30% Formula I compound·spinetobacillus thrips wettable powder (1:1) significantly outperformed the other agents in both rapid-acting and long-lasting efficacy. The 20% Formula I compound·spinosad water dispersible granules (2:3) had the highest efficacy, at 96.29%.

[0288] Example 8: Field efficacy test for controlling yellow striped flea beetle

[0289] Test basis: This test was conducted with reference to GB / T 17980.18-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for the control of yellow-striped flea beetles on cruciferous vegetables".

[0290] Test location: The test was conducted in a vegetable field in Hengshan Village, Lanxi City, Zhejiang Province. The test site is sandy loam, flat, with convenient drainage and irrigation, and the soil fertility is above average.

[0291] Experimental crops: pak choy (Shanghai green).

[0292] Target of prevention and control: Yellow striped flea beetle.

[0293] Experimental design: The experiment set up 5 chemical treatments and 1 clear water control. Each treatment was repeated 4 times, and each plot was 25m2. 2 , the plots were arranged in random blocks.

[0294] Application time: The test was conducted once on March 26, 2023, using a Gongnong-16 sprayer to evenly spray the entire plant. The weather on the day of application was cloudy, with temperatures ranging from 7°C to 17°C. There were no adverse weather conditions that could have affected the test results throughout the test period.

[0295] Survey time: Before the test, and 2 days and 7 days after the application of pesticides, samples were collected at 5 points in each experimental plot, with 4 plants at each point, for a total of 20 vegetables. The number of live insects was investigated and recorded, and the insect population reduction rate and control effect were calculated.

[0296] Calculation method of drug efficacy:

[0297]

[0298] Field efficacy test results:

[0299] Table 11 Results of field trials on efficacy of yellow striped flea beetles

[0300]

[0301] After the application of the pesticides, the entire process was recorded and compared. It was found that 2 days and 7 days after the spraying of each compound preparation, the rapeseed grew well, the sprayed drug dosage was within the safe range, and did not affect the growth of the seedlings. The control effect on the yellow flea beetle was ideal, ensuring the healthy growth of rapeseed.

[0302] In summary, through indoor toxicity tests and field efficacy tests, it can be seen that the pesticide composition of the present invention has a good control effect on Lepidoptera, Thysanoptera and Coleoptera pests, is safe for target crops, has significant control effects, and is superior to a single agent in delaying the development of resistance and prolonging the effectiveness.

[0303] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. A pesticide composition containing a biogenic insecticide, characterized in that: The active ingredients of the pesticide composition include a compound of formula I and a biogenic insecticide. The structure of the compound of formula I is as follows: (Formula I), the biosource insecticide is either spinosad or ethyl spinetoram, the mass ratio of the compound of Formula I to ethyl spinetoram is 1:42~32:1, and the mass ratio of the compound of Formula I to spinosad is 1:42~42:

1.

2. The pesticide composition according to claim 1, characterized in that The total weight of the pesticide composition is 100 wt%, and the total weight of the compound of formula I and the biogenic insecticide accounts for 0.01% to 80% of the total weight of the pesticide composition.

3. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also contains auxiliary ingredients allowed in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

4. The pesticide composition according to claim 1, characterized in that The pesticide composition is prepared into a formulation form permitted in pesticides, and the formulation form is a solid preparation or a liquid preparation.

5. The pesticide composition according to claim 4, characterized in that The solid preparation is a water-dispersible granule or a wettable powder; the liquid preparation is a suspension, a microemulsion, a dispersible oil suspension or an aqueous emulsion.

6. Use of the pesticide composition according to any one of claims 1 to 5 for controlling agricultural, horticultural or forestry pests.

7. The use according to claim 6, characterized in that The pesticide composition or formulation according to any one of claims 1 to 5 is applied to the pests to be controlled or the medium in which they grow.

Images