An oil control repair microemulsion for regulating the flora of oily sensitive skin and a preparation method and application thereof

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to an oil-controlling and repairing microemulsion for regulating the flora of oily sensitive skin as well as a preparation method and application thereof. Specifically, the oil-controlling and repairing microemulsion is directed against the flora of oily sensitive skin and the physiological characteristics of the skin, regulates propionibacterium which is prone to cause skin problems of the oily sensitive skin, thereby regulating the skin flora structure, and simultaneously improves the oil secretion and skin barrier of the oily sensitive skin, so as to achieve the effect of oil control and repair. It is also unexpectedly found in the present application that the formed oil-controlling and repairing emulsion can promote the proliferation of functional skin bacteria Staphylococcus epidermidis, and has a synergistic effect on relieving the flora imbalance caused by the proliferation of propionibacterium of oily skin acne, thus having good practical application value.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an oil-controlling and repairing microemulsion that regulates the microbial flora of oily and sensitive skin, its preparation method, and its application. Background Technology

[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] According to Mintel data, among current skincare consumption trends, sensitive combination and sensitive oily skin types account for the largest proportion of sensitive skin types surveyed. Research and consumer data show that oily sensitive skin individuals have different skincare needs compared to dry and combination sensitive skin types. The skin barrier damage and other skin problems caused by oily skin urgently need to be addressed.

[0004] Under the combined influence of internal hormone levels and external stimuli, sebaceous glands in oily skin often exhibit abnormal activity. The action of 5α-reductase stimulates the differentiation of sebaceous gland cells. The boundary between epidermal keratinocytes and sebaceous glands determines the direction of cell development. Sebaceous gland cells proliferate, promoting lipid synthesis. After lipid droplet synthesis, they gradually accumulate. Sebaceous gland cells undergo nuclear degradation and cell membrane rupture, releasing the lipid droplets through ducts to the skin surface, forming sebum. Propionibacterium acnes, which feeds on sebum, is a symbiotic flora of normal healthy skin and is itself a beneficial bacterium. However, in cases of excessive sebum secretion, the massive proliferation of Propionibacterium acnes activates the NLRP3 inflammasome within human sebaceous gland cells, promoting the expression of inflammatory factors. Increased sebum secretion and changes in the skin microbiota (skin barrier damage) dynamically alter the communication between the immune system and the skin microbiota, leading to changes in the expression of inflammatory factors. In acne cases, the expression of cytokines that interact with homeostasis is significantly increased; for example, the expression of TNF-α, IL-6, IL-8, and IL-1 in keratinocytes is significantly elevated, increasing pro-inflammatory responses. As a highly abundant genus of Propionibacterium in the skin, the proliferation of Propionibacterium acnes increases its abundance, causing a decrease in the abundance of other microbiota, resulting in a significant change in the overall skin microbiome composition, skin microbiota imbalance, and various skin problems. Currently, there is no effective solution that simultaneously addresses skin microbiota balance, sebum secretion regulation, and barrier function repair caused by skin microbiota and sebum imbalance leading to skin barrier damage. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention aims to provide an oil-controlling and repairing microemulsion for regulating the microbiome of oily and sensitive skin, along with its preparation method and applications. Specifically, the oil-controlling and repairing solution of this invention is prepared using microemulsion composite technology. Through cell experiments and human evaluations, it has been shown to promote the expression of filaggrin, naphthalene, and transglutamine 1; simultaneously, it can regulate the skin microbiome of oily skin, reducing the abundance of Propionibacterium and increasing the α-diversity of bacteria on the skin surface. The prepared oil-controlling and repairing solution has naturally derived active ingredients, and its efficacy is enhanced through the microemulsion process, resulting in highly effective, significant, gentle, and safe effects. Furthermore, this invention unexpectedly discovered that the resulting oil-controlling and repairing skincare solution can promote the proliferation of the functional skin bacterium Staphylococcus epidermidis, synergistically alleviating the microbiome imbalance caused by the proliferation of Propionibacterium acnes in oily skin. Based on the above research results, this invention is thus completed.

[0006] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0007] In a first aspect, the present invention provides an oil-controlling and repairing microemulsion for regulating the microbiome of oily and sensitive skin, the oil-controlling and repairing microemulsion comprising the following components in mass fraction (w / w):

[0008] Microemulsion emulsifier: 10-15%; oil: 4-30%; butylene glycol: 15-22%; patchouli extract: 0.2-1.0%; ethoxydiethylene glycol: 0.5-2.0%; white willow bark fermentation broth: 15-30%, balance being water.

[0009] A second aspect of the present invention provides a method for preparing the above-mentioned oil-controlling and repairing microemulsion, the method comprising:

[0010] S1: Mix microemulsion emulsifier, oil, and patchouli extract and heat to 65-80°C to obtain the first solution;

[0011] S2: Mix butanediol, ethoxydiethylene glycol, white willow bark fermentation broth and purified water and heat to 65-75℃ to form the second solution;

[0012] S3: Turn on the stirring and add the second solution to the first solution under high-speed homogenization;

[0013] S4: Continue high-speed homogenization for a certain period of time, then cool down to obtain a skin care lotion with oil-controlling and repairing effects.

[0014] A third aspect of the present invention provides the application of the above-mentioned oil-controlling and repairing microemulsion in the preparation of cosmetics.

[0015] A fourth aspect of the present invention provides a cosmetic product comprising at least the aforementioned oil-controlling and repairing microemulsion. The cosmetic product is a skincare product.

[0016] A fifth aspect of the present invention provides the use of the above-described oil-controlling and repairing microemulsion and / or cosmetic in any one or more of the following:

[0017] (a) Oil control and repair effects;

[0018] (b) Regulate skin flora and improve skin flora structure;

[0019] (c) Improve skin condition.

[0020] The beneficial technical effects of one or more of the above technical solutions are as follows:

[0021] 1. The skin care liquid obtained by the above technical solution can promote the expression of filaggrin, natriuretic protein and transglutamine 1 gene through cell experiments and human evaluation.

[0022] 2. The skin care liquid obtained by the above technical solution can inhibit the proliferation of Propionibacterium acnes. The product has been tested on oily and sensitive people and can reduce the abundance of Propionibacterium spp. while increasing the α-diversity of bacteria on the skin surface.

[0023] 3. Oil-controlling and repairing skin lotions can improve the physical and biological barriers of oily and sensitive skin, significantly repair the skin condition of oily and sensitive skin, and reduce sebum secretion and moisture loss.

[0024] 4. An unexpected discovery of the above technical solution is that the resulting oil-controlling and repairing skin care liquid can promote the proliferation of functional skin bacteria Staphylococcus epidermidis, which has a synergistic effect on alleviating the flora imbalance caused by the proliferation of Propionibacterium acnes in oily skin.

[0025] Meanwhile, the preparation method of the oil-controlling and repairing skin care liquid obtained by the above technical solution is simple and easy to implement, and the raw materials are inexpensive and easy to obtain, making it very suitable for industrial mass production, and therefore has good practical application value. Attached Figure Description

[0026] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0027] Figure 1 Comparison of physiological parameters between healthy skin group (HS) and oily sensitive skin group (OSS).

[0028] Figure 2 Species composition and differential species analysis (genus level) of healthy skin group (HS) and oily sensitive skin group (OSS).

[0029] Figure 3 Heatmap showing the correlation between skin physiological parameters, diversity index, and skin microbiota.

[0030] Figure 4 The effect of different samples on the growth of Propionibacterium acnes.

[0031] Figure 5 The effects of different oil-controlling and repairing solutions on the transcription of skin barrier factors.

[0032] Figure 6 The effect of different samples on the growth of Staphylococcus epidermidis.

[0033] Figure 7 : Skin bacterial species composition (genus level) before and after the tester used the sample.

[0034] Figure 8 Subject 2 showed improvement in sebum secretion before and after use.

[0035] Figure 9 Subject 2 showed improvement in barrier repair before and after treatment.

[0036] Figure 10 Subject 5 showed improvement in sebum secretion before and after use.

[0037] Figure 11 Subject 5 showed improvement in barrier repair before and after use. Detailed Implementation

[0038] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0039] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0040] In a typical embodiment of the present invention, an oil-controlling and repairing microemulsion for regulating the microbiome of oily and sensitive skin is provided, wherein the oil-controlling and repairing microemulsion comprises the following components in mass fraction (w / w):

[0041] Microemulsion emulsifier: 10-15%; oil: 4-30%; butylene glycol: 15-22%; patchouli extract: 0.2-1.0%; ethoxydiethylene glycol: 0.5-2.0%; white willow bark fermentation broth: 15-30%, balance being water.

[0042] The microemulsion emulsifier is potassium lauroyl wheat amino acid.

[0043] The oil is one or more of isononyl isononanoate, jojoba oil, dioctyl carbonate, and shea butter, preferably isononyl isononanoate and / or jojoba oil.

[0044] The patchouli extract can be prepared by the following method:

[0045] Patchouli was extracted by reflux with ethanol, then mixed with water and extracted with ether and n-butanol to obtain a concentrated extract, which is the patchouli extract. This patchouli extract has excellent antioxidant and whitening effects.

[0046] The mass ratio of patchouli to ethanol is 1:1-10, preferably 1:4, and the ethanol is high-concentration ethanol, such as 95% ethanol; the reflux extraction temperature is low, such as 25-35℃; the number of reflux extractions can be 1-3 times, preferably 2 times; in a specific embodiment of the present invention, the concentrated liquids after the two extractions are combined and dissolved with the same volume of water to obtain crude patchouli extract; then it is extracted 1-3 times with 2-4 times the volume of diethyl ether and n-butanol respectively, and the solvent is removed by concentration to obtain patchouli extract.

[0047] Fermented white willow bark broth can be prepared using the following method:

[0048] After the bark of white willow is crushed, sieved, and irradiated for sterilization, it is added to a culture medium and sterilized at high temperature. Then, lactic acid bacteria are inoculated into the medium for anaerobic fermentation to obtain a fermentation broth. The fermentation broth is then centrifuged and filtered to obtain the final product.

[0049] The sieve mesh size is 80-120 mesh, or more specifically 100 mesh.

[0050] The culture medium can be any bacterial culture medium, further specifically a culture medium for culturing lactobacilli, and even further specifically a modified MRS culture medium, the formula of which is as follows: 10g tryptone, 10g beef extract, 5g yeast extract, 20g glucose, 80ml Tween, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g citrate, 0.02g magnesium sulfate heptahydrate, 0.05g magnesium sulfate tetrahydrate, 1000ml distilled water, pH (5.6-5.8).

[0051] The volume ratio of the white willow bark to the culture medium is 1:1 to 1:3.

[0052] The inoculation amount of lactobacillus is 0.5% to 2.5% (v / v).

[0053] In this invention, the lactobacillus is *Lactiplantibacillus plantarum*, and in one specific embodiment, it is *Lactiplantibacillus plantarum* CICC 25125. Fermentation treatment using the above-mentioned lactobacillus yields a white willow bark fermentation liquid with superior anti-wrinkle, anti-inflammatory, and acne-reducing effects.

[0054] The anaerobic fermentation treatment specifically involves nitrogen-filled, stirred culture for 36-54 hours.

[0055] In another specific embodiment of the present invention, a method for preparing the above-mentioned oil-controlling and repairing microemulsion is provided, the preparation method comprising:

[0056] S1: Mix microemulsion emulsifier, oil, and patchouli extract and heat to 65-80°C to obtain the first solution;

[0057] S2: Mix butanediol, ethoxydiethylene glycol, white willow bark fermentation broth and water and heat to 65-75°C to obtain the second solution;

[0058] S3: Start stirring and add the second solution to the first solution under high-speed homogenization until complete;

[0059] S4: Continue high-speed homogenization for a certain period of time, then cool down to obtain a skin care lotion with oil-controlling and repairing effects.

[0060] In another specific embodiment of the present invention, in step S3, the stirring speed is 300-500 r / min. Stirring makes the material uniform, and high-speed homogenization plays an emulsifying role. At the same time, stirring also makes the homogenization and emulsification more uniform. The high-speed homogenization speed is 8000-10000 r / min.

[0061] In another specific embodiment of the present invention, in step S4, the high-speed homogenization speed is 8000-10000 r / min, and the homogenization time is 5-10 min.

[0062] This invention's oil-controlling and repairing emulsion targets the microbiome and physiological characteristics of oily and sensitive skin. It regulates the Propionibacterium spp., which is prone to causing skin problems in oily and sensitive skin, thereby adjusting the skin's microbiome structure. Simultaneously, it improves sebum secretion and the skin barrier, achieving oil control and repair effects. Furthermore, the invention unexpectedly discovered that the resulting oil-controlling and repairing emulsion can promote the proliferation of the functional skin bacterium Staphylococcus epidermidis, synergistically alleviating the microbiome imbalance caused by the proliferation of Propionibacterium acnes in oily skin.

[0063] Therefore, in another specific embodiment of the present invention, the application of the above-mentioned oil-controlling and repairing microemulsion in the preparation of cosmetics is provided.

[0064] In another specific embodiment of the present invention, a cosmetic product is provided, which at least comprises the above-mentioned oil-controlling and repairing microemulsion. The cosmetic product is a skincare product.

[0065] Obviously, the cosmetics may also contain other ingredients permitted in any cosmetic field, such as emollients, moisturizers, antioxidants, etc., which will not be elaborated here.

[0066] Furthermore, by rationally adding the above-mentioned raw material components, this invention can also be used to prepare different cosmetic formulations, such as essence water, essence lotion, essence cream, etc. In addition, based on the above-mentioned cosmetic categories, other cosmetic categories can be further derived and prepared, which obviously also fall within the protection scope of this application.

[0067] In another specific embodiment of the present invention, the above-mentioned oil-controlling and repairing microemulsion and / or cosmetic is provided for use in any one or more of the following:

[0068] (a) Oil control and repair effects;

[0069] (b) Regulate skin flora and improve skin flora structure;

[0070] (c) Improve skin condition.

[0071] The present invention will be further described below with reference to embodiments. The present invention is further illustrated by means of embodiments, but this does not limit the present invention to the scope of the embodiments described. Based on the embodiments of the present invention, any changes to the present invention by those skilled in the art without inventive step are within the scope of protection of the present invention. Furthermore, in the embodiments of the present invention, unless otherwise specified, all raw materials used in preparation are commercially available products well known to those skilled in the art. The preparation method of the Propionibacterium acnes CCSM0331 and Staphylococcus epidermidis CCSM0287 seed culture used in the embodiments is as follows:

[0072] Bacterial powders of *Propionibacterium acnes* CCSM0331 and *Staphylococcus epidermidis* CCSM0287 were inoculated onto TSA plates using a disposable sterile inoculation loop and incubated aerobically at 37°C for 16-20 hours. Single colonies were picked and streaked onto the plates and incubated at 37°C for 16-20 hours. Purified single colonies were then picked from the plates and inoculated into TSB liquid medium and incubated with shaking at 37°C for 16-20 hours. The bacterial seed culture was obtained when the OD600 value was between 0.5 and 1.0 and was ready for use. Both strains were obtained through screening by Shandong Freda Biotechnology Co., Ltd.

[0073] Example 1: Analysis of differences in skin flora and skin physiological parameters between oily-skinned, sensitive individuals and healthy, non-sensitive individuals.

[0074] A questionnaire survey was used to allow volunteers to self-assess their skin sensitivity, and the groups who perceived themselves as sensitive or non-sensitive were identified. Sebum levels were then measured, with those exceeding 120 μg / cm³ being the most affected. 2 The participants were considered to have oily skin. Among the 182 volunteers, those with self-perceived non-oily skin (not sensitive) served as the healthy control group (Healthy skin, HS, 17 people), and those with self-perceived sensitive oily skin served as the oily sensitive skin group (Oily Sensitive skin, OOS, 20 people). Compared to the healthy skin group, as... Figure 1 The results showed that people with oily and sensitive skin had significantly lower skin moisture content and b-value (P < 0.05), significantly higher transepidermal water loss rate, sebum, heme, and skin roughness (P < 0.05), and significantly larger pore area (P < 0.05). Skin microbiota diversity analysis showed that compared with healthy skin, oily and sensitive skin had lower microbiota diversity. Species composition analysis showed that the abundance of Propionibacterium was significantly increased, while the abundance of Rhodococcus and Pseudomonas was significantly decreased (P < 0.001). Spearman correlation analysis showed that skin sebum secretion was positively correlated with Propionibacterium.

[0075] Example 2: Preparation of Oil Control and Repair Solution

[0076] Preparation of fermentation broth of lactic acid bacteria from white willow bark: White willow bark was ultrafinely pulverized and passed through a 100-mesh sieve. After being sterilized by plasma beam irradiation, it was added to MRS medium at a volume ratio of 1:2, mixed and stirred, and sterilized by high-pressure steam at 121℃ for 30 min. Then, Lactobacillus plantarum CICC 25125 (purchased from China Industrial Microbial Culture Collection Center) was inoculated at a volume ratio of 0.6%, and cultured under nitrogen with stirring for 48 h to obtain the fermentation broth. The fermentation broth was centrifuged at 8000 r / min for 30 min and filtered through a 0.22 μm filter membrane to obtain the fermentation broth of lactic acid bacteria from white willow bark.

[0077] Preparation of patchouli extract: Patchouli was soaked in 95% ethanol at a mass ratio of 1:4. After soaking in a sealed container with shaking for 2 hours, the solution was filtered under vacuum at 29 degrees Celsius to obtain concentrated solution I. After recovering the ethanol, the solution was soaked in a sealed container with shaking for 2 hours. After filtration under vacuum at 29 degrees Celsius, concentrated solution II was obtained. Concentrated solutions I and II were combined and dissolved in purified water at a volume ratio of 1:1 to obtain crude patchouli extract. The crude extract was then extracted twice with three times its volume of diethyl ether and n-butanol, respectively. The solvent was removed by concentration to obtain the extract concentrate.

[0078] The specific preparation method is as follows:

[0079] S1: Weigh each component according to the weight percentage of each raw material in Table 1;

[0080] S2: Mix the microemulsion emulsifier lauroyl wheat amino acid potassium, oil (isononyl isononanoate / jojoba oil / shea butter), and patchouli extract and heat to 75°C to form solution one;

[0081] S3: Mix butanediol, ethoxydiethylene glycol, white willow bark fermentation broth and purified water and heat to 70°C to form solution two;

[0082] S4: Start stirring (400r / min) to make the material uniform, and at the same time, slowly add solution 2 to solution 1 under high-speed homogenization (9000r / min);

[0083] S5: After completely adding solution 2 to solution 1, continue high-speed homogenization (9000r / min) for 5 minutes, and then cool to room temperature to obtain the skin care lotion with oil-controlling and repairing effects.

[0084] The proportions of each component in Examples 3-8 and Comparative Examples 1-5 are shown in Table 1 below.

[0085] Table 1. List of components and contents in the examples and comparative examples.

[0086]

[0087] Example 9: Effects of different samples on the growth of Propionibacterium acnes

[0088] Preparation of Propionibacterium acnes seed culture: Powdered Propionibacterium acnes bacteria were inoculated onto TSA plates using a disposable sterile inoculation loop and incubated at 37°C for 16-20 hours. Single colonies were picked and streaked onto second-generation plates and aerobically incubated at 37°C for 16-20 hours. Purified single colonies were picked from the second-generation plates and inoculated into TSB liquid medium and incubated at 37°C with shaking for 16-20 hours. When the OD600 value was between 0.5 and 1.0, the bacterial seed culture was obtained and ready for use.

[0089] The control group was set up with 48 mL of TSB medium, and the different sample groups were set up with a mixture of 4.8 mL of sample (Examples 2-8, Comparative Examples 1-4) + 43.2 mL of TSB as the medium. The seed culture was inoculated at a rate of 2% into 50 mL centrifuge tubes containing different sample mediums and placed in a 37°C incubator for anaerobic shaking culture for 72 h.

[0090] After cultivation, the fermentation broth was shaken well, and the cultured Propionibacterium acnes bacterial suspension in the experimental group was diluted 1:10 six times using sterile PBS to obtain 10 -1 ~10 -6 For the diluted bacterial solution, take 10... -4 ~10-5 Plate counting was performed. 100 μL of the corresponding dilution of bacterial suspension was added to a TSA agar plate containing 5% defibrinated sheep blood and spread evenly using a spreader. Three replicates were performed for each dilution. The control group underwent the same procedure. All plates were incubated anaerobicly at 37℃ for 72 h. Plate counts were performed, and the total colony count was calculated. The relative proliferation rate of each sample group compared to the control group was calculated using the following formula: Relative proliferation rate (%) = (Sample group bacterial count - Control group bacterial count) / Control group bacterial count × 100%. The results are shown below. Figure 4 As shown, Examples 2-8 all exhibited inhibitory effects on the proliferation of Propionibacterium acnes. Comparative Example 4 demonstrates that, compared to Example 5, the oil-controlling and repairing solution containing shea butter was also insufficient to inhibit the proliferation of Propionibacterium acnes induced by caprylic / capric triglycerides.

[0091] Example 10: Effects of different oil-controlling and repairing solutions on the transcription of skin barrier factors

[0092] Human keratinocytes (HaCaT) were processed at a rate of 2.0 × 10⁻⁶. 5 The cells were seeded at a density of 1 cell / well into 6-well plates. The control group used high-glucose DMEM medium, the sample group used high-glucose DMEM medium containing 0.25% test sample, and the positive control group used high-glucose DMEM medium containing 50 μM WY14643. All samples were incubated with HaCaT at 37°C and 5% CO2 for 24 hours. Real-time quantitative PCR was then performed sequentially using TAKARA's RNA extraction kit, RNA reverse PCR kit, and TB Green quantitative PCR kit to determine the relative expression levels of filaggrin gene FLG, lobe protein gene LOR, and glutamine transferase TGM1. The results are as follows: Figure 5 As shown in the figure. Experiments have demonstrated that Example 7 significantly promoted the expression of all three genes, and the effects were superior to those of the other examples, and significantly superior to Comparative Examples 1-5.

[0093] The primer sequences for gene fluorescence quantitative PCR are as follows:

[0094] FLG F: 5'-GCCAGGGACAATCAGAGG-3' (SEQ ID NO.1),

[0095] FLG R: 5'-TGGAAGCAGACCCAGACC-3' (SEQ ID NO. 2);

[0096] LOR F 5'-TCATGATGCTACCCGAGGTTTG-3' (SEQ ID NO. 3);

[0097] LOR R 5'-TGCAAATTTATTGACTGAGGCACTG-3' (SEQ ID NO. 4);

[0098] TGM1 F 5'-TTACAGAGGCCCAAGATCCTCAAC-3' (SEQ ID NO.5),

[0099] TGM1 R 5'-TCCAAGCTGGCAATGAGCTG-3' (SEQ ID NO. 6).

[0100] Example 11: Effects of different oil-controlling and repairing solutions on the growth of Staphylococcus epidermidis.

[0101] Preparation of Staphylococcus epidermidis seed culture: Staphylococcus epidermidis CCSM0287 bacterial powder was inoculated onto TSA plates using a disposable sterile inoculation loop and then placed in a 37°C constant temperature incubator for aerobic incubation for 16-20 h. Single colonies were picked and streaked onto second-generation plates and placed in a 37°C constant temperature incubator for aerobic incubation for 16-20 h. Purified single colonies were picked from the second-generation plates and inoculated into TSB liquid medium and placed in a 37°C shaker for shaking incubation for 16-20 h. The seed culture was obtained when the OD600 value was between 0.5 and 1.0 and was ready for use.

[0102] The control group was set up with 45 mL of TSB medium, and the different sample groups were set up with a mixture of 4.5 mL of sample (Examples 2-8, Comparative Examples 1-5) + 40.5 mL of TSB as the medium. The seed culture was inoculated at a rate of 2% into 50 mL centrifuge tubes containing different sample mediums and placed in a 37°C incubator for aerobic shaking culture for 72 h.

[0103] After cultivation, the fermentation broth was shaken well, and the cultured Staphylococcus epidermidis suspension was diluted 1:10 six times with sterile PBS to obtain 10... -1 ~10 -6 For the diluted bacterial solution, take 10... -4 ~10 -5 Plate counting was performed by adding 100 μL of the corresponding dilution of bacterial suspension to a TSA agar plate containing 5% defibrinated sheep blood, spreading it evenly using a spreader, and performing three replicates for each dilution. The control group underwent the same procedure. All plates were incubated anaerobically at 37°C for 72 h, and plate counts were performed to calculate the total bacterial count. The relative proliferation rate of each sample compared to the control group was calculated using the following formula:

[0104] Relative proliferation rate (%) = (number of bacteria in the sample group - number of bacteria in the control group) / number of bacteria in the control group × 100%

[0105] Example 12: Effects of oil-controlling and repairing products on the microbiome of oily, sensitive skin.

[0106] A serum product was prepared by suspending 10% (by mass) of an oil-controlling and repairing solution (Example 5) in a thickening system. Volunteers aged 18-25 with oily and sensitive facial skin were used as subjects. The participants used the serum containing the oil-controlling and repairing solution continuously for 28 days. The beneficial effects of the serum on the skin microbiota were evaluated by measuring changes in the skin flora before and after product use. Using the serum containing this emulsion significantly reduced the abundance of Propionibacterium, increased the bacterial species composition of oily and sensitive skin, and brought the skin microbiota towards a balanced state.

[0107] Example 13: The effect of oil-controlling and repairing liquid products on improving oily and sensitive skin.

[0108] A serum product was prepared by suspending 10% (by weight) of an oil-controlling and repairing solution (Example 5) in a thickening system. Volunteers aged 18-25 with oily and sensitive facial skin were used as subjects. The participants used the serum containing the oil-controlling and repairing solution continuously for 28 days. The improvement effect on oily and sensitive skin was evaluated by measuring sebum secretion and redness improvement before and after product use. Using a product containing the oil-controlling and repairing solution can inhibit sebum secretion in oily and sensitive skin and repair the skin barrier function.

[0109] It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the given examples, those skilled in the art can modify or make equivalent substitutions to the technical solutions of the present invention as needed, without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. An oil-controlling and repairing microemulsion for regulating the microbiome of oily and sensitive skin, characterized in that, The oil-controlling and repairing microemulsion contains the following components by mass fraction: Microemulsion emulsifier: 10-15%; oil: 4-30%; butylene glycol: 15-22%; patchouli extract: 0.2-1.0%; ethoxydiethylene glycol: 0.5-2.0%; white willow bark fermentation broth: 15-30%, balance being water; The microemulsion emulsifier is lauroyl wheat amino acid potassium; The oil contains A and B; A is shea butter; B is one or more of isononyl isononanoate and jojoba oil. The fermented liquid from white willow bark was prepared using the following method: After the bark of white willow is crushed, sieved, and irradiated for sterilization, it is added to a culture medium and sterilized at high temperature. Then, lactic acid bacteria are inoculated into the medium for anaerobic fermentation to obtain a fermentation broth. The fermentation broth is then centrifuged and filtered to obtain the final product.

2. The oil-controlling and repairing microemulsion as described in claim 1, characterized in that, The patchouli extract was prepared by the following method: After being extracted by reflux with ethanol, patchouli was mixed with water and then extracted with ether and n-butanol to obtain the concentrated extract, which is the patchouli extract.

3. The oil-controlling and repairing microemulsion as described in claim 1, characterized in that, The sieve mesh size is 80-120 mesh.

4. The oil-controlling and repairing microemulsion as described in claim 3, characterized in that, The sieve mesh size is 100 mesh.

5. The oil-controlling and repairing microemulsion as described in claim 1, characterized in that, The culture medium is a modified MRS medium.

6. The oil-controlling and repairing microemulsion as described in claim 1, characterized in that, The volume ratio of the white willow bark to the culture medium is 1:1 to 1:3; The inoculation amount of lactobacillus is 0.5%~2.5% ( v / v ); The anaerobic fermentation treatment specifically involves nitrogen-filled, stirred culture for 36-54 hours.

7. The method for preparing the oil-controlling and repairing microemulsion according to any one of claims 1-6, characterized in that, The preparation method includes: S1: Mix microemulsion emulsifier, oil, and patchouli extract and heat to 65~80℃ to obtain the first solution; S2: Mix butanediol, ethoxydiethylene glycol, white willow bark fermentation broth and purified water and heat to 65~75℃ to obtain the second solution; S3: Start stirring and add the second solution to the first solution under high-speed homogenization until complete; S4: Continue high-speed homogenization for a certain period of time, then cool down to obtain a skin care lotion with oil-controlling and repairing effects.

8. The preparation method of the oil-controlling and repairing microemulsion as described in claim 7, characterized in that, In step S3, the stirring speed is 300~500 r / min, and the high-speed homogenization speed is 8000~10000 r / min.

9. The preparation method of the oil-controlling and repairing microemulsion as described in claim 7, characterized in that, In step S4, the high-speed homogenization speed is 8000~10000 r / min, and the homogenization time is 5~10 min.

10. The use of the oil-controlling and repairing microemulsion according to any one of claims 1-6 in the preparation of cosmetics.

11. A cosmetic product, characterized in that, The cosmetic product comprises at least the oil-controlling and repairing microemulsion as described in any one of claims 1-6.

12. The cosmetic product as described in claim 11, characterized in that, The cosmetics in question are skincare products.

13. The use of the oil-controlling and repairing microemulsion according to any one of claims 1-6 and / or the cosmetic according to any one of claims 11-12 in any one or more of the following: (a) Oil control and repair effects; (b) Regulate the skin flora and improve the structure of the skin flora; (c) Improve skin condition; The application is for non-disease treatment purposes.

Images