Preimplantation genetic diagnosis embryo biopsy device

Abstract

The present invention discloses a preimplantation genetic diagnosis embryo biopsy device, and relates to the technical field of medical equipment. The main points of the technical solution are: comprising a base, an embryo placement table, and a sample placement table; the top surface of the base is fixedly provided with a first support column and a second support column; the embryo placement table comprises a chassis, a heating plate, and a heat conducting plate, the chassis is a cylindrical groove structure with an opening at the top, the heating plate and the heat conducting plate are sequentially embedded in the chassis, the chassis is rotatably connected to the first support column, and the top surface of the heat conducting plate is provided with a plurality of first placement grooves; the sample placement table comprises a placement table, the placement table is rotatably connected to the second support column, and the top surface of the placement table is provided with a plurality of second placement grooves; the chassis is movably connected to the placement table. The present invention can accurately associate sampling, and can directly perform PGS detection after sampling, and through a significant marking structure, accurately and clearly mark the culture dish containing healthy and normal embryos after the detection, so as to quickly identify normal embryos and improve the success rate of embryo transplantation.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and more particularly to a preimplantation genetic diagnosis embryo biopsy device. Background Art

[0002] Preimplantation Genetic Screening (PGS1) refers to the detection of chromosome number and structural abnormalities in early embryos before implantation. It is an early prenatal screening method that detects the structure and number of 23 pairs of chromosomes in the embryo at one time to analyze whether the embryo has genetic abnormalities, so as to select normal embryos and implant them into the uterus in order to obtain a normal pregnancy, improve the patient's clinical pregnancy rate, and reduce multiple pregnancies.

[0003] Patent CN113322185A discloses a pre-implantation genetic diagnosis embryo biopsy device, including an incubation table, a transparent cover and a rotating seat. The incubation table is provided with a plurality of culture stations, each of which has an incubation tank suitable for placing a culture dish and a sampling tank suitable for inserting a sampling tube; the transparent cover is located above the incubation table, and the transparent cover is provided with an opening suitable for revealing a culture station; the rotating seat is arranged at the bottom of the incubation table, and is used to drive the incubation table to rotate relative to the transparent cover, so as to selectively switch one of the culture stations to the opening. The pre-implantation genetic diagnosis embryo biopsy device provided according to an embodiment of the present invention can be used for biopsy sampling, ensure the correlation between the sample and the embryo in the culture dish, and reduce the possible risks caused by human operation errors.

[0004] The existing device ensures the correlation between the sample and the embryo in the culture dish by setting up interrelated culture stations and an opening that only exposes one culture station. Although such a device ensures the correlation between the sample and the embryo in the culture dish when sampling, usually, PGS testing is performed directly after the sample is taken out. When the present device needs to perform PGS testing on the sample, the sampling tube still needs to be taken out, which is not only inconvenient, but also there is still a risk of matching errors after the test. Summary of the invention

[0005] The purpose of the present invention is to solve the above-mentioned problems and provide a preimplantation genetic diagnosis embryo biopsy device. The incubation table and sampling table are designed to be interrelated but independent of each other. PGS detection can be directly performed after sampling, and a significant marking structure is added. After the PGS detection is completed, the culture dish containing healthy and normal embryos can be clearly marked, and medical personnel can identify normal embryos very quickly, greatly improving the success rate of embryo transplantation.

[0006] The above technical objectives of the present invention are achieved through the following technical solutions: a pre-implantation genetic diagnosis embryo biopsy device, comprising a base, an embryo placement table, and a sample placement table; the top surface of the base is fixedly provided with a first support column and a second support column; the embryo placement table comprises a chassis, a heating plate, and a heat conducting plate, the chassis is a cylindrical groove structure with an opening at the top, the heating plate and the heat conducting plate are sequentially embedded in the chassis, the chassis is rotatably connected to the first support column, and the top surface of the heat conducting plate is provided with a plurality of first placement grooves; the sample placement table comprises a placement table, the placement table is rotatably connected to the second support column, and the top surface of the placement table is provided with a plurality of second placement grooves; the chassis is movably connected to the placement table.

[0007] By adopting the above technical solution, firstly, the device is fixed, and the storage table is placed close to the detection instrument, for example, at a position convenient for microscope observation, and then the embryo culture dishes that need to be tested by PGS are placed in multiple first storage slots respectively, and the heating plate can ensure the appropriate temperature required for embryo culture; then multiple sample culture dishes are placed in multiple second storage slots respectively, and the storage table is driven to rotate by rotating the chassis. When one of the first storage slots and the second storage slots is close to each other, the rotation is stopped, and the embryos in the embryo culture dishes are sampled and placed in the sample culture dishes. When all the embryos are sampled, each sample culture dish is directly tested in turn by the detection instrument, and after the test is completed, the embryo corresponding to the sample that is tested normally can be transplanted; the whole process does not require multiple transfers of the sample culture dishes, the operation is convenient, and the risk of embryo matching errors after the test can be effectively reduced.

[0008] The present invention is further configured as follows: the first storage slots and the second storage slots are the same in number and are equidistantly arranged along the circumference of the heat conducting plate and the storage table; and the first storage slots and the second storage slots are both provided with mutually symmetrical notches.

[0009] By adopting the above technical solution, setting the same number of first storage slots and second storage slots can ensure that the embryo culture dish and the sample culture dish correspond one to one when sampling; symmetrical notches are set on the first storage slot and the second storage slot to facilitate the placement and removal of the culture dish.

[0010] The present invention is further configured as follows: the top surface of the heat conduction plate is provided with a plurality of groups of signal light groups corresponding to the plurality of the first storage slots, each group of the signal light groups includes two signal lights of different colors; the top surface of the storage table is provided with a plurality of groups of switch groups corresponding to the plurality of the second storage slots, each of the switch groups includes two switches, and the plurality of the switch groups and the plurality of the signal light groups are connected one-to-one through electrical signals, and among the correspondingly connected switch groups and signal light groups, two switches and two signal lights are connected one-to-one.

[0011] By adopting the above technical solution, a signal light group is used to distinguish and mark normal embryos from abnormal embryos, which is convenient for doctors to identify and avoid mistakes. The specific operation is: after testing a sample in a sample culture dish, according to the test result, if it is normal, press the switch in the switch group representing that the sample test is normal, and the signal light in the corresponding signal light group indicating that the embryo is normal will light up, and the embryo culture dish will be marked as normal; otherwise, press the switch in the switch group representing that the sample test is abnormal, and the signal light indicating that the embryo is abnormal will light up, and the embryo culture dish will be marked as abnormal; when all samples are tested, the embryos in the embryo culture dish marked as normal can be selected for transplantation, and the remaining normal embryos can continue to be refrigerated and stored; this marking method is simple and fast, and is very convenient for doctors to identify, which greatly improves the success rate of embryo transplantation.

[0012] The present invention is further configured as follows: a limiting column is provided in the chassis, a protrusion is provided on the side of the limiting column, and limiting holes following the shape of the limiting column are provided at the bottom of the heat conducting sheet and the heat conducting plate.

[0013] By adopting the above technical solution, the disassembly and installation of various components of the device are facilitated while the first storage groove is positioned.

[0014] The present invention is further configured as follows: a first sleeve is fixedly provided on the bottom surface of the chassis, the first sleeve is sleeved on the top of the first support column, and the first sleeve is fixedly connected to a first gear ring.

[0015] The present invention is further configured as follows: the first support column is fixedly connected to a first support plate close to the first sleeve, the first support plate is rotatably connected to a first gear, the first gear is meshed with the first gear ring, the first gear shaft is connected to a motor, and the motor is fixed to the top surface of the base.

[0016] The present invention is further configured as follows: a second sleeve is fixedly connected to the bottom of the storage table, the second sleeve is rotatably sleeved on the top of the second support column, and the second sleeve is fixedly connected to a second gear ring.

[0017] The present invention is further configured as follows: the second support column is fixedly connected to a second support plate, the second support plate is rotatably connected to a second gear, and the second gear is meshed with both the first gear and the second gear ring.

[0018] The present invention is further configured such that: the first gear ring and the second gear ring have completely the same structure and size, and the first gear and the second gear have completely the same structure and size.

[0019] By adopting the above technical solution, the motor is started to drive the first gear to rotate, and the first gear ring, the second gear, and the second gear ring are driven to rotate, and then the chassis and the storage table are driven to rotate. Under the action of the limiting column, the heat conduction plate rotates. Since the first gear ring and the second gear ring have exactly the same structure and size, and the first gear and the second gear have exactly the same structure and size, the multiple first storage slots and the multiple second storage slots can rotate at the same speed and correspond one by one in sequence.

[0020] The present invention is further configured as follows: a plurality of L-shaped optical coupling baffles are equidistantly arranged on the bottom surface of the chassis along the circumferential direction, the number of the optical coupling baffles is the same as the number of the first storage slots, and the positions correspond one to one; the first support column is fixedly connected to a third support plate, the third support plate is fixedly connected to a photoelectric switch, the plurality of optical coupling baffles circulate through the photoelectric switch in sequence, and the photoelectric switch is electrically connected to the motor.

[0021] By adopting the above technical solution, when multiple optical coupling baffles pass through the photoelectric switch in sequence, the motor will be prompted to stop running. Since the first gear ring, the first gear, the second gear and the second gear ring are engaged in sequence, the heat conduction plate and the storage table will rotate and stop synchronously. Each time they stop, the related embryo culture dishes and sample culture dishes will approach each other for sampling, thereby ensuring the correlation of the samples after sampling and avoiding matching errors in the detection results.

[0022] In summary, the present invention has the following beneficial effects:

[0023] The present invention drives the first gear to rotate through a motor, and transmits the first gear ring, the second gear, and the second gear ring to rotate, and then drives the heat conduction plate and the storage table to rotate. Since the first gear ring and the second gear ring have exactly the same structure and size, and the first gear and the second gear have exactly the same structure and size, a plurality of first storage slots and a plurality of second storage slots can rotate at the same speed and correspond one by one in sequence; by arranging a photoelectric switch and an optical coupling baffle corresponding to the plurality of first storage slots, when the plurality of optical coupling baffles pass through the photoelectric switch in sequence, the motor will be prompted to stop running, and since the first gear ring, the first gear, the second gear, and the second gear ring are meshed in sequence, the heat conduction plate and the storage table will rotate and stop synchronously, and each time they stop, mutually related embryo culture dishes and sample culture dishes will approach each other for sampling, thereby ensuring the correlation of samples after sampling and avoiding matching errors in detection results.

[0024] The present invention places the storage table close to the detection instrument, for example, at a position convenient for observation with a microscope. When all embryos have been sampled, each sample culture dish is directly tested in turn by the detection instrument. After the test is completed, the embryo corresponding to the sample with normal test result can be transplanted. The whole process does not require multiple transfers of sample culture dishes, the operation is convenient, and the risk of incorrect embryo matching after the test can be effectively reduced.

[0025] The present invention utilizes a signal light group to distinguish and mark normal embryos from abnormal embryos, so as to facilitate identification by doctors and avoid misremembering. The specific operation is as follows: after detecting a sample in a sample culture dish, according to the detection result, if it is normal, the switch in the switch group representing that the sample detection is normal is pressed, and at this time, the signal light in the corresponding signal light group representing that the embryo is normal is lit, and the embryo culture dish is marked as normal; otherwise, the switch in the switch group representing that the sample detection is abnormal is pressed, and the signal light representing that the embryo is abnormal is lit, and the embryo culture dish is marked as abnormal; after all samples are detected, the embryos in the embryo culture dish marked as normal are selected for transplantation, and the remaining normal embryos can continue to be refrigerated and stored; this marking method is simple and quick, and is very convenient for doctors to identify, and greatly improves the success rate of embryo transplantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a structural diagram of a device in an embodiment of the present invention (top perspective);

[0027] Figure 2 is a structural diagram of a device in an embodiment of the present invention (bottom perspective);

[0028] Figure 3 is an exploded view of a device in an embodiment of the present invention;

[0029] Figure 4 is a bottom structural diagram of a chassis in an embodiment of the present invention;

[0030] Figure 5 This is a bottom structural diagram of a storage table in an embodiment of the present invention;

[0031] Figure 6 1 is a bottom structural diagram of the heat conducting plate in an embodiment of the present invention.

[0032] In the figure: 1. base; 2. embryo placement table; 21. chassis; 22. heating plate; 23. heat conducting plate; 24. first storage slot; 211. limiting column; 212. first sleeve; 213. first gear ring; 25. limiting hole; 3. sample placement table; 31. placement table; 311. second sleeve; 312. second gear ring; 32. second storage slot; 4. first support column; 41. first support plate; 42. first gear; 43. motor; 5. second support column; 51. second support plate; 52. second gear; 6. notch; 7. signal light group; 8. switch group; 9. optical coupling baffle; 10. third support plate; 11. photoelectric switch; 12. embryo culture dish; 13. sample culture dish. DETAILED DESCRIPTION

[0033] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described in detail below in conjunction with the embodiments of the present invention and the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0034] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present invention can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0035] Example:

[0036] like Figures 1 to 6 As shown, the embryo biopsy device for preimplantation genetic diagnosis comprises a base 1, an embryo placement table 2, and a sample placement table 3; a first support column 4 and a second support column 5 are installed on the top surface of the base 1; the embryo placement table 2 comprises a bottom plate 21, a heating plate 22, and a heat conducting plate 23, the bottom plate 21 is a cylindrical groove structure with an opening at the top, a limiting column 211 is installed in the bottom plate 21, a convexity is provided on the side of the limiting column 211, and a limiting hole 25 which is shaped like the limiting column 211 is provided at the bottom of the heat conducting plate and the heat conducting plate 23, and the heating plate 22 and the heat conducting plate 23 are sequentially embedded in the bottom plate 21 through the limiting column 211;

[0037] A first sleeve 212 is installed on the bottom surface of the chassis 21, and the first sleeve 212 is sleeved on the top of the first support column 4. The first sleeve 212 is fixedly connected to the first gear ring 213. The first support column 4 is fixedly connected to a first support plate 41 close to the first sleeve 212, and the first support plate 41 is rotatably connected to a first gear 42, which is meshed with the first gear ring 213. The first gear 42 is axially connected to a motor 43, and the motor 43 is fixed to the top surface of the base 1.

[0038] The sample placement table 3 includes a placement table 31, a second sleeve 311 is fixedly connected to the bottom of the placement table 31, the second sleeve 311 is rotatably sleeved on the top of the second support column 5, and the second sleeve 311 is fixedly connected to the second gear ring 312. The second support column 5 is fixedly connected to the second support plate 51, and the second support plate 51 is rotatably connected to the second gear 52, and the second gear 52 is meshed with the first gear 42 and the second gear ring 312. The first gear ring 213 and the second gear ring 312 are completely identical in structure and size, and the first gear 42 and the second gear 52 are completely identical in structure and size.

[0039] The motor 43 is started to drive the first gear 42 to rotate, and the first gear ring 213, the second gear 52, and the second gear ring 312 are driven to rotate, and then the chassis 21 and the storage table 31 are driven to rotate. Under the action of the limiting column 211, the heat conducting plate 23 rotates. Since the first gear ring 213 and the second gear ring 312 have exactly the same structure and size, and the first gear 42 and the second gear 52 have exactly the same structure and size, the multiple first storage slots 24 and the multiple second storage slots 32 can all rotate at the same speed and correspond one by one in sequence.

[0040] A plurality of L-shaped optical coupling baffles 9 are installed equidistantly along the circumferential direction on the bottom surface of the chassis 21. The number of the optical coupling baffles 9 is the same as the number of the first storage slots 24, and the positions correspond one to one. The first support column 4 is fixedly connected to the third support plate 10, and the third support plate 10 is fixedly connected to the photoelectric switch 11. The plurality of optical coupling baffles 9 circulate through the photoelectric switch 11 in sequence, and the photoelectric switch 11 is electrically connected to the motor 43. When the plurality of optical coupling baffles 9 pass through the photoelectric switch 11 in sequence, the motor 43 will be prompted to stop running. Since the first gear ring 213, the first gear 42, the second gear 52 and the second gear ring 312 are meshed in sequence, the heat conducting plate 23 and the storage table 31 will rotate and stop synchronously. Each time they stop, the embryo culture dish 12 and the sample culture dish 13 that are related to each other will approach each other for sampling, so as to ensure the correlation of the samples after sampling and avoid matching errors in the detection results.

[0041] The top surface of the heat conducting plate 23 is provided with a plurality of first storage slots 24; the top surface of the storage platform 31 is provided with a plurality of second storage slots 32, the plurality of first storage slots 24 and the plurality of second storage slots 32 are the same in number, and are respectively arranged equidistantly along the circumference of the heat conducting plate 23 and the storage platform 31, the embryo culture dishes 12 that need to be tested by PGS are respectively placed in the plurality of first storage slots 24, and the heating plate 22 can ensure the appropriate temperature required for embryo culture; the plurality of sample culture dishes 13 are respectively placed in the plurality of second storage slots 32; the plurality of first storage slots 24 and the plurality of second storage slots 32 are both provided with mutually symmetrical notches 6. The same number of first storage slots 24 and second storage slots 32 can ensure that when sampling, the embryo culture dishes 12 and the sample culture dishes 13 correspond one to one; the mutually symmetrical notches 6 are provided to facilitate the placement and removal of the culture dishes.

[0042] A plurality of signal light groups 7 corresponding to the plurality of first storage slots 24 are installed on the top surface of the heat conducting plate 23, and each signal light group 7 includes two signal lights of different colors, representing normal embryos and abnormal embryos respectively; a plurality of switch groups 8 corresponding to the plurality of second storage slots 32 are provided on the top surface of the storage table 31, and each switch group 8 includes two switches, and the plurality of switch groups 8 and the plurality of signal light groups 7 are connected one-to-one through electrical signals, and in the correspondingly connected switch groups 8 and signal light groups 7, the two switches and the two signal lights are connected one-to-one. The signal light group 7 is used to distinguish and mark normal embryos from abnormal embryos, which is convenient for doctors to identify and avoid mistakes. The specific operation is: after testing a sample in a sample culture dish 13, according to the test result, if it is normal, the switch representing that the sample test is normal in the switch group 8 is pressed, and the signal light representing that the embryo is normal in the corresponding signal light group 7 is lit, and the embryo culture dish 12 is marked as normal; otherwise, the switch representing that the sample test is abnormal in the switch group 8 is pressed, and the signal light representing that the embryo is abnormal is lit, and the embryo culture dish 12 is marked as abnormal; after all samples are tested, the embryos in the embryo culture dish 12 marked as normal are selected for transplantation, and the remaining normal embryos can continue to be refrigerated and stored; this marking method is simple and fast, and is very convenient for doctors to identify, which greatly improves the success rate of embryo transplantation.

[0043] Working principle: first fix the device, place the storage table 31 close to the detection instrument, for example, a position convenient for microscope observation, start the motor 43 to drive the heat conduction plate 23 and the storage table 31 to rotate, and under the control of the optical coupling baffle and the photoelectric switch 11, when one of the first storage slots 24 and the second storage slot 32 is close to each other, stop the rotation, and place the embryo samples in the embryo culture dish 12 in the corresponding sample culture dish 13. When all the embryos are sampled, the detection instrument is used to directly detect each sample culture dish 13 in turn. Each time a sample is detected, the corresponding embryo condition is marked as normal or abnormal. After the detection is completed, the marked normal embryos can be selected for transplantation, and the remaining normal embryos are refrigerated.

[0044] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A preimplantation genetic diagnosis embryo biopsy device, characterized by: The invention comprises a base (1), an embryo placement table (2), and a sample placement table (3); a first support column (4) and a second support column (5) are fixedly provided on the top surface of the base (1); the embryo placement table (2) comprises a bottom plate (21), a heating plate (22), and a heat conducting plate (23); the bottom plate (21) is a cylindrical groove structure with an opening at the top; the heating plate (22) and the heat conducting plate (23) are sequentially embedded in the bottom plate (21); the bottom plate (21) is rotatably connected to the first support column (4); a plurality of first storage grooves (24) are provided on the top surface of the heat conducting plate (23); the sample placement table (3) comprises a storage table (31); the storage table (31) is rotatably connected to the second support column (5); a plurality of second storage grooves (32) are provided on the top surface of the storage table (31); the bottom plate (21) is movably connected to the storage table (31); The plurality of first storage slots (24) and the plurality of second storage slots (32) are of the same number and are respectively arranged equidistantly along the circumference of the heat conducting plate (23) and the storage platform (31); the plurality of first storage slots (24) and the plurality of second storage slots (32) are both provided with mutually symmetrical notches (6); A first sleeve (212) is fixedly provided on the bottom surface of the chassis (21), the first sleeve (212) is sleeved on the top of the first support column (4), and the first sleeve (212) is fixedly connected to a first gear ring (213); The first support column (4) is fixedly connected to a first support plate (41) close to the first sleeve (212); the first support plate (41) is rotatably connected to a first gear (42); the first gear (42) is meshed with the first gear ring (213); the first gear (42) is axially connected to a motor (43); and the motor (43) is fixed to the top surface of the base (1); The bottom of the storage platform (31) is fixedly connected to a second sleeve (311), the second sleeve (311) is rotatably sleeved on the top of the second support column (5), and the second sleeve (311) is fixedly connected to a second gear ring (312); The second support column (5) is fixedly connected to a second support plate (51), the second support plate (51) is rotatably connected to a second gear (52), and the second gear (52) is meshed with both the first gear (42) and the second gear ring (312); The first gear ring (213) and the second gear ring (312) have completely the same structure and size, and the first gear (42) and the second gear (52) have completely the same structure and size; The bottom surface of the chassis (21) is provided with a plurality of L-shaped optical coupling baffles (9) equidistantly along the circumferential direction, the number of the optical coupling baffles (9) being the same as the number of the first storage slots (24), and the positions being corresponding one to one; the first support column (4) is fixedly connected to a third support plate (10), the third support plate (10) is fixedly connected to a photoelectric switch (11), the plurality of optical coupling baffles (9) circulate through the photoelectric switch (11) in sequence, and the photoelectric switch (11) is electrically connected to the motor (43).

2. The preimplantation genetic diagnosis embryo biopsy device according to claim 1, characterized in that: The top surface of the heat conducting plate (23) is provided with a plurality of groups of signal light groups (7) corresponding to the plurality of the first storage slots (24), each group of the signal light groups (7) comprising two signal lights of different colors; the top surface of the storage platform (31) is provided with a plurality of groups of switch groups (8) corresponding to the plurality of the second storage slots (32), each of the switch groups (8) comprising two switches, and the plurality of the switch groups (8) and the plurality of the signal light groups (7) are connected one-to-one through electrical signals, and in the correspondingly connected switch groups (8) and signal light groups (7), two switches and two signal lights are connected one-to-one.

3. The preimplantation genetic diagnosis embryo biopsy device according to claim 1, characterized in that: A limiting column (211) is provided in the chassis (21), a protrusion is provided on the side of the limiting column (211), and a limiting hole (25) which is shaped like the limiting column (211) is provided at the bottom of the heat conducting plate (23).

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