A microfluidic bioassay device for efficient cell and virus culture

Abstract

The present invention relates to the technical field of efficient cell and virus culture, and discloses a microfluidic biological detection device for efficient cell and virus culture, including an incubator, the incubator is provided with a buffer mechanism, the buffer mechanism includes a buffer oblique cavity opened in the incubator, a bar box is fixedly installed on the top of the buffer oblique cavity, a bar groove is opened on the back of the bar box, and also includes: a plurality of leakage holes are opened on the bar box, the plurality of leakage holes all penetrate the bar box, a buffer plate is slidably installed in the bar groove, and a buffer rod is fixedly installed on the front of the buffer plate. When the washing liquid through groove of the present invention enters the buffer cavity, the impact force will also be reduced during the turn, so that the flow rate and pressure of the washing liquid are greatly reduced, and then the washing liquid will slowly flow out from the oblique groove to flush the culture medium in the buffer oblique cavity, and the decompression and flow rate reduction of the washing liquid can effectively avoid the occurrence of cell membrane rupture, organelle damage or cell shedding due to excessive flow rate.

Description

Technical Field

[0001] The invention relates to the technical field of efficient cell and virus culture equipment, in particular to a microfluidic biological detection device for efficient cell and virus culture. Background Art

[0002] The interaction between cell microenvironment and cells has increasingly become one of the research hotspots in the field of cell biology. Manipulating, observing and detecting single cells cultured in vitro is a common method for exploring cell biological behavior. Microfluidic chip devices with height, lateral and longitudinal scales in the order of micrometers, millimeters or centimeters are not only ideal experimental platforms for capturing and manipulating cells cultured in vitro, but also important experimental tools for constructing microfluidic biomechanical and biochemical environments for cells cultured in vitro, and have been widely used in cell biology research.

[0003] When conducting microfluidic testing on cells or (viruses), the culture medium needs to be discarded, washed three times with a buffer solution, the original culture medium needs to be rinsed clean and new culture medium containing drugs needs to be replaced. During the washing process of the culture dish, the flow rate and pressure of the buffer solution cannot be reduced, otherwise a flow rate that is too fast will damage the cells, while a flow rate that is too slow will not be able to rinse the original culture medium clean, which will cause cell growth to be hindered and affect the detection effect. Summary of the invention

[0004] The purpose of the present invention is to provide a microfluidic biological detection device for efficient cell and virus culture, so as to solve the problem that it is impossible to reduce the flow rate pressure of the buffer solution during the washing process of the culture dish, otherwise a too fast flow rate will damage the cells, while a too slow flow rate will not be able to rinse the original culture medium clean, thereby causing cell growth to be hindered and affecting the detection effect.

[0005] In order to solve the above technical problems, the present invention is achieved through the following technical solutions:

[0006] The present invention is a microfluidic biological detection device for efficient cell and virus culture, comprising an incubator, the incubator being provided with a buffer mechanism, the buffer mechanism comprising an oblique buffer cavity provided in the incubator, a bar box being fixedly mounted on the top of the oblique buffer cavity, a bar groove being provided on the back of the bar box, and further comprising:

[0007] The strip box is provided with a plurality of leakage holes, and the plurality of leakage holes all penetrate the strip box; a buffer plate is slidably installed in the strip groove, a buffer rod is fixedly installed on the front side of the buffer plate, and the front end of the buffer rod extends outside the strip box; two buffer cavities are provided on the strip box, and a plurality of through grooves are respectively provided on the inner walls of the two buffer cavities on one side close to each other, and the plurality of through grooves are communicated with the strip groove; and a plurality of inclined grooves are respectively provided on the inner walls of the front sides of the two buffer cavities.

[0008] Furthermore, an oscillation mechanism is provided in the buffer oblique cavity, and the oscillation mechanism includes two oscillation slide bars fixedly installed in the buffer oblique cavity, two oscillation plates are slidably installed on the two oscillation slide bars, two oscillation springs are respectively sleeved on the two oscillation slide bars, ends of several oscillation springs that are away from each other are fixedly connected to the inner wall of the buffer oblique cavity, ends of several oscillation springs that are close to each other are respectively fixedly connected to the two oscillation plates, connecting rods are respectively hingedly installed on the two oscillation plates, and the ends of the two connecting rods are hinged to the buffer rod.

[0009] Furthermore, a stirring mechanism is respectively provided on the two oscillation plates, and the stirring mechanism comprises a mounting groove opened on the oscillation plate, a plurality of stirring rods are rotatably mounted in the mounting groove, and a plurality of stirring plates are respectively fixedly mounted on the plurality of stirring rods.

[0010] Furthermore, a driving mechanism is provided on the right side of the incubator, and the driving mechanism includes a driving motor fixedly installed on the right side of the incubator. A driving rod is provided in the incubator, and driving rods are rotatably installed on the left and right sides of the incubator respectively, and the ends of the two driving rods close to each other extend into the driving groove, the output shaft of the driving motor is fixedly connected to the driving rod close to the driving motor, and driving plates are fixedly installed on the ends of the two driving rods close to each other respectively, and connecting round rods are fixedly installed on the two driving plates, and movable plates are hingedly installed on the connecting round rods.

[0011] Furthermore, an extrusion mechanism is provided in the driving groove, and the extrusion mechanism includes a buffer box fixedly mounted on the inner wall at the top of the driving groove, an extrusion plate is slidably mounted in the buffer box, an extrusion rod is fixedly mounted on the front side of the extrusion plate, the front end of the extrusion rod extends out of the buffer box, the front end of the extrusion rod is hinged to the movable plate, a liquid outlet pipe is fixedly mounted on the back side of the buffer box, and the top end of the liquid outlet pipe extends into the buffer oblique cavity.

[0012] Furthermore, a circulation mechanism is provided on the front of the incubator, and the circulation mechanism includes a circulation box fixedly installed on the front of the incubator, a baffle is slidably installed in the circulation box, and the top of the baffle extends outside the circulation box, and liquid inlet pipes are fixedly installed on the left and right sides of the circulation box, respectively, and the two liquid inlet pipes are fixedly connected to the buffer box.

[0013] Further, a lifting mechanism is provided on the corresponding driving rod. The lifting mechanism includes a semi-gear fixedly sleeved on the driving rod away from the driving motor. A U-shaped frame is fixedly installed on the left side of the incubator. A toothed plate is slidably sleeved on the U-shaped frame. The toothed plate meshes with the semi-gear. A fixing plate is fixedly sleeved on the U-shaped frame. A lifting spring is sleeved on the U-shaped frame. The top end of the lifting spring is fixedly connected to the fixing plate, and the bottom end of the lifting spring is fixedly connected to the toothed plate. A connecting slide rod is fixedly installed on the front surface of the toothed plate, and the end of the connecting slide rod is fixedly connected to the baffle.

[0014] Further, a detection mechanism is provided on the top of the incubator. The detection mechanism includes a top cover provided on the top of the incubator, and a transparent plate is fixedly installed on the top cover.

[0015] The present invention has the following beneficial effects:

[0016] (1) For the microfluidic biodetector for efficient cell and virus culture of the present invention, when the cells in the buffer inclined cavity are placed in the buffer inclined cavity for 24 hours and the cells are close to the inner wall of the buffer inclined cavity, the driving motor is started. The driving motor drives the driving rod to rotate, the driving rod drives the driving plate to rotate, the driving plate drives the connecting round rod to rotate, the connecting round rod drives the movable plate to move, the movable plate drives the extrusion rod to move, the extrusion rod will move towards the buffer box, the extrusion rod drives the extrusion plate to move, and the extrusion plate squeezes the washing liquid in the buffer box into the liquid outlet pipe and discharges it into the buffer inclined cavity through the liquid outlet pipe. The washing liquid entering the buffer inclined cavity will pass through the strip box, contact the culture medium in the buffer inclined cavity, and wash out the original culture medium outside the buffer inclined cavity, preparing for placing the culture medium containing drugs;

[0017] (2) For the microfluidic biodetector for efficient cell and virus culture of the present invention, when the liquid outlet pipe discharges into the buffer inclined cavity, since the diameter of the liquid outlet pipe is smaller than that of the buffer box, the pressure and flow rate of the liquid discharged from the liquid outlet pipe will increase. The washing liquid will impact the buffer plate in the strip groove. After being impacted, the buffer plate will drive the buffer rod to move towards the oscillating slide rod. When the buffer plate moves a certain distance, several through grooves will be exposed. At this time, the washing liquid will enter the buffer cavity from the through grooves. During this process, the buffer plate will reduce the pressure of the washing liquid. When the washing liquid enters the buffer cavity from the through grooves, it will also reduce the impact force during turning, greatly reducing the flow rate and pressure of the washing liquid. Then the washing liquid will slowly flow out from the inclined groove and wash the culture medium in the buffer inclined cavity. The decompression and flow rate reduction of the washing liquid can effectively avoid the occurrence of cell membrane rupture, organelle damage or cell detachment caused by too high a flow rate;

[0018] (3) The present invention provides a microfluidic biological detection device for efficient cell and virus culture. When the washing liquid impacts the buffer plate, the buffer plate drives the buffer rod to move, the buffer rod drives the two connecting rods to move away from each other, and the connecting rod drives the two oscillation plates to move away from each other. At this time, a plurality of oscillation springs will be compressed. During the movement of the oscillation plate, the washing liquid in the buffer oblique cavity will be pushed to both sides, which will cause the washing liquid to oscillate and flush the culture medium in the buffer oblique cavity. During the continuous rotation of the driving plate, the extrusion rod will move back and forth, so the washing liquid sprayed out of the liquid outlet pipe will also be intermittent. When the liquid outlet pipe stops spraying, the oscillation The plates will drive the two oscillating plates to approach each other under the elastic force of the oscillating spring. When the oscillating plates approach each other, they will also cause oscillation to make the washing liquid in the buffer oblique cavity flush the culture liquid. In the reciprocating motion of the extrusion rod, in cooperation with the oscillating spring, the oscillating plates will continuously approach and move away from each other, and flush the culture medium in the buffer oblique cavity through oscillation. In the process of the oscillating plates approaching and moving away from each other, several stirring rods will be driven to rotate. When the oscillating plates approach and move away from each other, since the stirring plates are already in the washing liquid, the movement of the oscillating plates will drive several stirring plates to rotate. The rotation of the stirring plates has a stirring effect, which can better clean the culture medium in the buffer oblique cavity.

[0019] (4) The present invention provides a microfluidic biological detection device for efficient cell and virus culture. During the rotation of the driving rod, the half gear will be driven to rotate, and the half gear will drive the tooth plate to rise. The tooth plate will drive the connecting slide rod to rise, and the connecting slide rod will drive the baffle to rise. During the continuous rotation of the driving rod, when the tooth plate leaves the teeth on the half gear, the tooth plate will drive the baffle to descend under the elastic force of the lifting spring. During the lifting and lowering of the baffle, the washing liquid after flushing will enter the circulation box and flow back to the buffer box through two liquid inlet pipes to achieve the effect of recycling. When the culture medium in the buffer oblique chamber is flushed clean, the tooth plate is raised and the driving motor is turned off. At this time, the washing liquid in the buffer oblique chamber will pass through the circulation box and enter the buffer box to prepare for the next cleaning of the culture medium.

[0020] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0023] Figure 2It is a schematic diagram of the cross-sectional structure of the internal part of the present invention;

[0024] Figure 3 For the present invention Figure 2 A is a schematic diagram of the enlarged structure of the middle part;

[0025] Figure 4 For the present invention Figure 2 Schematic diagram of the enlarged structure of B;

[0026] Figure 5 It is a schematic diagram of the front section structure of the present invention;

[0027] Figure 6 It is a schematic diagram of the side cross-sectional structure of the internal part of the present invention;

[0028] Figure 7 It is a schematic diagram of the structure of the driving mechanism of the present invention;

[0029] Figure 8 It is a bottom view structural schematic diagram of the present invention.

[0030] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0031] In the figure: 1. incubator; 100. buffer mechanism; 101. buffer oblique cavity; 102. strip box; 103. strip groove; 104. leak hole; 105. buffer plate; 106. buffer rod; 107. buffer cavity; 108. through groove; 109. oblique groove; 2. oscillation mechanism; 201. oscillation slide bar; 202. oscillation plate; 203. oscillation spring; 204. connecting rod; 3. stirring mechanism; 301. mounting groove; 302. stirring rod; 303. stirring plate; 4. driving mechanism; 401. driving motor; 402. driving Groove; 403, driving rod; 404, driving plate; 405, connecting round rod; 406, movable plate; 5, extrusion mechanism; 501, buffer box; 502, extrusion plate; 503, extrusion rod; 504, liquid outlet pipe; 6, circulation mechanism; 601, circulation box; 602, baffle; 603, liquid inlet pipe; 7, lifting mechanism; 701, half gear; 702, profile frame; 703, tooth plate; 704, fixed plate; 705, lifting spring; 706, connecting slide rod; 8, detection mechanism; 801, top cover; 802, transparent plate. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] See also Figure 1-Figure 8 As shown, the present invention is a microfluidic biological detection device for efficient cell and virus culture, comprising an incubator 1, on which a buffer mechanism 100 is provided, the buffer mechanism 100 comprises an oblique buffer chamber 101 provided in the incubator 1, a bar box 102 is fixedly installed on the top of the oblique buffer chamber 101, and a bar groove 103 is provided on the back of the bar box 102, and further comprises:

[0034] A plurality of leakage holes 104 are provided on the strip box 102, and the plurality of leakage holes 104 all penetrate the strip box 102. A buffer plate 105 is slidably installed in the strip groove 103, and a buffer rod 106 is fixedly installed on the front of the buffer plate 105. The front end of the buffer rod 106 extends to the outside of the strip box 102. Two buffer cavities 107 are provided on the strip box 102, and a plurality of through grooves 108 are respectively provided on the inner walls of the two buffer cavities 107 on one side close to each other, and the plurality of through grooves 108 are communicated with the strip groove 103, and a plurality of inclined grooves 109 are respectively provided on the inner walls of the front sides of the two buffer cavities 107.

[0035] like Figure 2 As shown, an oscillation mechanism 2 is provided in the buffer oblique cavity 101, and the oscillation mechanism 2 includes two oscillation slide bars 201 fixedly installed in the buffer oblique cavity 101, and two oscillation plates 202 are slidably installed on the two oscillation slide bars 201. Two oscillation springs 203 are respectively sleeved on the two oscillation slide bars 201, and the ends of several oscillation springs 203 that are away from each other are fixedly connected to the inner wall of the buffer oblique cavity 101, and the ends of several oscillation springs 203 that are close to each other are respectively fixedly connected to the two oscillation plates 202, and connecting rods 204 are respectively hingedly installed on the two oscillation plates 202, and the ends of the two connecting rods 204 are hinged to the buffer rod 106.

[0036] When the washing liquid impacts the buffer plate 105, the buffer plate 105 drives the buffer rod 106 to move, the buffer rod 106 drives the two connecting rods 204 to move away from each other, and the connecting rod 204 drives the two oscillation plates 202 to move away from each other. At this time, several oscillation springs 203 will be compressed, and the oscillation plate 202 will push the washing liquid in the buffer oblique chamber 101 to both sides during the movement, which will cause the washing liquid to oscillate and flush the culture medium in the buffer oblique chamber 101.

[0037] like Figure 4 As shown, a stirring mechanism 3 is respectively provided on the two oscillation plates 202 , and the stirring mechanism 3 includes a mounting groove 301 opened on the oscillation plate 202 , a plurality of stirring rods 302 are rotatably mounted in the mounting groove 301 , and a plurality of stirring plates 303 are respectively fixedly mounted on the plurality of stirring rods 302 .

[0038] In the process of the oscillation plates 202 approaching and moving away from each other, the plurality of stirring rods 302 will be driven to rotate. When the oscillation plates 202 approach and move away from each other, since the stirring plates 303 are already in the washing liquid, the movement of the oscillation plates 202 will drive the plurality of stirring plates 303 to rotate. The rotation of the stirring plates 303 has a stirring effect, which can better clean the culture medium in the buffer oblique chamber 101.

[0039] like Figure 5 and Figure 6 As shown, a driving mechanism 4 is provided on the right side of the incubator 1, and the driving mechanism 4 includes a driving motor 401 fixedly installed on the right side of the incubator 1, a driving rod 403 is provided in the incubator 1, and the driving rods 403 are rotatably installed on the left and right sides of the incubator 1 respectively, and the ends of the two driving rods 403 close to each other extend into the driving slot 402, the output shaft of the driving motor 401 is fixedly connected to the driving rod 403 close to the driving motor 401, and the ends of the two driving rods 403 close to each other are respectively fixedly installed with driving plates 404, and connecting round rods 405 are fixedly installed on the two driving plates 404, and movable plates 406 are hingedly installed on the connecting round rods 405.

[0040] The driving motor 401 drives the driving rod 403 to rotate, the driving rod 403 drives the driving plate 404 to rotate, the driving plate 404 drives the connecting round rod 405 to rotate, and the connecting round rod 405 drives the movable plate 406 to move.

[0041] like Figure 6 As shown, an extrusion mechanism 5 is provided in the driving groove 402, and the extrusion mechanism 5 includes a buffer box 501 fixedly installed on the inner wall at the top of the driving groove 402, an extrusion plate 502 is slidably installed in the buffer box 501, an extrusion rod 503 is fixedly installed on the front of the extrusion plate 502, the front end of the extrusion rod 503 extends to the outside of the buffer box 501, the front end of the extrusion rod 503 is hinged to the movable plate 406, and a liquid outlet pipe 504 is fixedly installed on the back of the buffer box 501, and the top end of the liquid outlet pipe 504 extends into the buffer oblique cavity 101.

[0042] The movable plate 406 drives the squeezing rod 503 to move, and the squeezing rod 503 moves toward the buffer box 501. The squeezing rod 503 drives the squeezing plate 502 to move, and the squeezing plate 502 squeezes the washing liquid in the buffer box 501 into the liquid outlet pipe 504, and discharges it into the buffer oblique chamber 101 through the liquid outlet pipe 504. The washing liquid entering the buffer oblique chamber 101 will pass through the strip box 102 and contact the culture medium in the buffer oblique chamber 101, and flush the original culture medium out of the buffer oblique chamber 101, so as to prepare for the placement of the culture medium containing the medicine.

[0043] like Figure 5As shown in the figure, a circulation mechanism 6 is provided on the front of the incubator 1. The circulation mechanism 6 includes a circulation box 601 fixedly installed on the front of the incubator 1. A baffle 602 is slidably installed in the circulation box 601. The top of the baffle 602 extends outside the circulation box 601. Liquid inlet pipes 603 are fixedly installed on the left and right sides of the circulation box 601 respectively. Both of the two liquid inlet pipes 603 are fixedly connected to the buffer tank 501.

[0044] During the lifting process of the baffle 602, the rinsed washing liquid will enter the circulation box 601 and flow back into the buffer tank 501 through the two liquid inlet pipes 603, achieving the effect of recycling. When the culture medium in the buffer inclined cavity 101 is rinsed clean, the toothed plate 703 is lifted, and the driving motor 401 is turned off. At this time, the washing liquid in the buffer inclined cavity 101 will enter the buffer tank 501 through the circulation box 601, preparing for the next cleaning of the culture medium.

[0045] As Figure 7 shown in the figure, a lifting mechanism 7 is provided on the corresponding driving rod 403. The lifting mechanism 7 includes a semi-gear 701 fixedly sleeved on the driving rod 403 far from the driving motor 401. A U-shaped frame 702 is fixedly installed on the left side of the incubator 1. A toothed plate 703 is slidably sleeved on the U-shaped frame 702. The toothed plate 703 meshes with the semi-gear 701. A fixing plate 704 is fixedly sleeved on the U-shaped frame 702. A lifting spring 705 is sleeved on the U-shaped frame 702. The top end of the lifting spring 705 is fixedly connected to the fixing plate 704. The bottom end of the lifting spring 705 is fixedly connected to the toothed plate 703. A connecting slide rod 706 is fixedly installed on the front of the toothed plate 703. The end of the connecting slide rod 706 is fixedly connected to the baffle 602.

[0046] During the rotation of the driving rod 403, the semi-gear 701 will be driven to rotate. The semi-gear 701 drives the toothed plate 703 to rise. The toothed plate 703 will drive the connecting slide rod 706 to rise. The connecting slide rod 706 drives the baffle 602 to rise. During the continuous rotation of the driving rod 403, when the toothed plate 703 leaves the teeth on the semi-gear 701, under the elastic force of the lifting spring 705, the toothed plate 703 will drive the baffle 602 to descend.

[0047] As Figure 1 shown in the figure, a detection mechanism 8 is provided on the top of the incubator 1. The detection mechanism 8 includes a top cover 801 provided on the top of the incubator 1. A transparent plate 802 is fixedly installed on the top cover 801.

[0048] Through the setting of the transparent plate 802, a microscope can be used to observe the cell growth situation in the buffer inclined cavity 101 through the pressing plate 502. The top cover 801 can prevent dust in the air from entering the buffer inclined cavity 101 and affecting the cell detection effect.

[0049] When in use, after the cells in the buffer oblique chamber 101 are placed in the buffer oblique chamber 101 for twenty-four hours, when the cells are close to the inner wall of the buffer oblique chamber 101, the driving motor 401 is started, the driving motor 401 drives the driving rod 403 to rotate, the driving rod 403 drives the driving plate 404 to rotate, the driving plate 404 drives the connecting round rod 405 to rotate, the connecting round rod 405 drives the movable plate 406 to move, the movable plate 406 drives the squeezing rod 503 to move, the squeezing rod 503 will move in the direction close to the buffer box 501, the squeezing rod 503 drives the squeezing plate 502 to move, the squeezing plate 502 squeezes the washing liquid in the buffer box 501 into the liquid outlet pipe 504, and discharges it into the buffer oblique chamber 101 through the liquid outlet pipe 504, the washing liquid entering the buffer oblique chamber 101 will pass through the bar box 102 to contact the culture medium in the buffer oblique chamber 101 and flush the original culture medium out of the buffer oblique chamber 101, so as to prepare for placing the culture medium containing drugs; The liquid pipe 504 is discharged into the buffer oblique chamber 101. Since the diameter of the liquid outlet pipe 504 is smaller than the diameter of the buffer box 501, the pressure and flow rate of the liquid discharged from the liquid outlet pipe 504 will increase, and the washing liquid will impact the buffer plate 105 in the strip groove 103. After the buffer plate 105 is impacted, it will drive the buffer rod 106 to move in the direction close to the oscillation slide bar 201. When the buffer plate 105 moves a certain distance, a number of through grooves 108 will be exposed. At this time, the washing liquid will enter the buffer chamber 107 from the through grooves 108. In the process again, the buffer plate 105 will reduce the pressure of the washing liquid. When the washing liquid through grooves 108 enter the buffer chamber 107, the impact force will also be reduced during the turn, so that the flow rate and pressure of the washing liquid are greatly reduced. Then, the washing liquid will slowly flow out from the oblique groove 109 to flush the culture medium in the buffer oblique chamber 101. The decompression and flow rate reduction of the washing liquid can effectively avoid the occurrence of cell membrane rupture, organelle damage or cell shedding due to excessive flow rate.

[0050] When the washing liquid impacts the buffer plate 105, the buffer plate 105 drives the buffer rod 106 to move, and the buffer rod 106 drives the two connecting rods 204 to move away from each other, and the connecting rod 204 drives the two oscillation plates 202 to move away from each other. At this time, a plurality of oscillation springs 203 will be compressed, and the oscillation plate 202 will push the washing liquid in the buffer oblique cavity 101 to both sides during the movement, which will cause the washing liquid to oscillate and flush the culture medium in the buffer oblique cavity 101. During the continuous rotation of the driving plate 404, the squeezing rod 503 will move back and forth, so that the liquid outlet pipe 504 sprays The washing liquid discharged may also be intermittent. When the liquid outlet pipe 504 stops spraying liquid, the oscillating plate 202 will drive the two oscillating plates 202 to approach each other under the elastic force of the oscillating spring 203. When the oscillating plates 202 approach each other, they will also cause oscillation to make the washing liquid in the buffer oblique cavity 101 flush the culture liquid. During the reciprocating motion of the squeezing rod 503, in cooperation with the oscillating spring 203, the oscillating plates 202 will continuously approach and move away from each other, and flush the culture medium in the buffer oblique cavity 101 through oscillation. In the process of the oscillating plates 202 approaching and moving away from each other, they will drive a number of stirring rods 302 rotates, and when the oscillating plates 202 move closer to each other, since the stirring plates 303 are already in the washing liquid, the movement of the oscillating plates 202 will drive the rotation of several stirring plates 303, and the rotation of the stirring plates 303 plays a stirring role, which can better clean the culture medium in the buffer oblique chamber 101; during the rotation of the driving rod 403, the half gear 701 will be driven to rotate, and the half gear 701 will drive the tooth plate 703 to rise, and the tooth plate 703 will drive the connecting slide rod 706 to rise, and the connecting slide rod 706 will drive the baffle 602 to rise. During the continuous rotation of the driving rod 403, when the tooth plate 703 When leaving the teeth on the half gear 701, the tooth plate 703 will drive the baffle 602 to descend under the elastic force of the lifting spring 705. During the lifting and lowering process of the baffle 602, the washing liquid after flushing will enter the circulation box 601 and flow back to the buffer box 501 through the two liquid inlet pipes 603, so as to achieve the effect of recycling. When the culture medium in the buffer oblique chamber 101 is flushed clean, the tooth plate 703 is raised and the drive motor 401 is turned off. At this time, the washing liquid in the buffer oblique chamber 101 will pass through the circulation box 601 and enter the buffer box 501, so as to prepare for the next cleaning of the culture medium.

[0051] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A microfluidic biological detection device for efficient cell and virus culture, comprising an incubator (1), wherein the incubator (1) is provided with a buffer mechanism (100), wherein the buffer mechanism (100) comprises a buffer oblique cavity (101) provided in the incubator (1), wherein a strip box (102) is fixedly mounted on the top of the buffer oblique cavity (101), and a strip groove (103) is provided on the back of the strip box (102), wherein: Also includes: The strip box (102) is provided with a plurality of leakage holes (104), and the plurality of leakage holes (104) all penetrate the strip box (102); a buffer plate (105) is slidably installed in the strip groove (103); a buffer rod (106) is fixedly installed on the front of the buffer plate (105); the front end of the buffer rod (106) extends outside the strip box (102); two buffer cavities (107) are provided on the strip box (102); a plurality of through grooves (108) are respectively provided on the inner walls of the two buffer cavities (107) on one side close to each other; the plurality of through grooves (108) are all communicated with the strip groove (103); a plurality of inclined grooves (109) are respectively provided on the inner walls of the front sides of the two buffer cavities (107); An oscillation mechanism (2) is arranged in the buffer oblique cavity (101), and the oscillation mechanism (2) comprises two oscillation slide bars (201) fixedly mounted in the buffer oblique cavity (101), two oscillation plates (202) being slidably mounted on the two oscillation slide bars (201), two oscillation springs (203) being respectively sleeved on the two oscillation slide bars (201), the ends of the plurality of oscillation springs (203) being away from each other being fixedly connected to the inner wall of the buffer oblique cavity (101), the ends of the plurality of oscillation springs (203) being close to each other being fixedly connected to the two oscillation plates (202), and connecting rods (204) being respectively hingedly mounted on the two oscillation plates (202), and the ends of the two connecting rods (204) being hingedly connected to the buffer rod (106); A stirring mechanism (3) is respectively arranged on the two oscillating plates (202), and the stirring mechanism (3) comprises a mounting groove (301) provided on the oscillating plate (202), a plurality of stirring rods (302) are rotatably mounted in the mounting groove (301), and a plurality of stirring plates (303) are respectively fixedly mounted on the plurality of stirring rods (302); A driving mechanism (4) is arranged on the right side of the incubator (1), the driving mechanism (4) comprising a driving motor (401) fixedly mounted on the right side of the incubator (1), a driving rod (403) is provided in the incubator (1), the driving rods (403) are rotatably mounted on the left and right sides of the incubator (1), the ends of the two driving rods (403) close to each other both extend into the driving slot (402), the output shaft of the driving motor (401) is fixedly connected to the driving rod (403) close to the driving motor (401), the ends of the two driving rods (403) close to each other are fixedly mounted with driving plates (404), the two driving plates (404) are fixedly mounted with connecting round rods (405), and the connecting round rods (405) are hingedly mounted with a movable plate (406); A pressing mechanism (5) is arranged in the driving groove (402). The pressing mechanism (5) includes a buffer box (501) fixedly installed on the inner wall of the top of the driving groove (402). A pressing plate (502) is slidably installed in the buffer box (501). A pressing rod (503) is fixedly installed on the front surface of the pressing plate (502). The front end of the pressing rod (503) extends outside the buffer box (501). The front end of the pressing rod (503) is hinged to the movable plate (406). A liquid outlet pipe (504) is fixedly installed on the back surface of the buffer box (501). The top end of the liquid outlet pipe (504) extends into the buffer inclined cavity (101).

2. A microfluidic biological detection device for efficient cell and virus culture according to claim 1, characterized in that: A circulation mechanism (6) is arranged on the front surface of the incubator (1). The circulation mechanism (6) includes a circulation box (601) fixedly installed on the front surface of the incubator (1). A baffle (602) is slidably installed in the circulation box (601). The top of the baffle (602) extends outside the circulation box (601). Liquid inlet pipes (603) are respectively fixedly installed on the left side and the right side of the circulation box (601). Both of the two liquid inlet pipes (603) are fixedly connected to the buffer box (501).

3. A microfluidic biological detection device for efficient cell and virus culture according to claim 2, characterized in that: A lifting mechanism (7) is arranged on the corresponding driving rod (403). The lifting mechanism (7) includes a semi-gear (701) fixedly sleeved on the driving rod (403) far from the driving motor (401). A U-shaped frame (702) is fixedly installed on the left side of the incubator (1). A toothed plate (703) is slidably sleeved on the U-shaped frame (702). The toothed plate (703) is meshed with the semi-gear (701). A fixing plate (704) is fixedly sleeved on the U-shaped frame (702). A lifting spring (705) is sleeved on the U-shaped frame (702). The top end of the lifting spring (705) is fixedly connected to the fixing plate (704). The bottom end of the lifting spring (705) is fixedly connected to the toothed plate (703). A connecting sliding rod (706) is fixedly installed on the front surface of the toothed plate (703). The end of the connecting sliding rod (706) is fixedly connected to the baffle (602).

4. A microfluidic biological detection device for efficient cell and virus culture according to claim 3, characterized in that: A detection mechanism (8) is arranged on the top of the incubator (1). The detection mechanism (8) includes a top cover (801) arranged on the top of the incubator (1). A transparent plate (802) is fixedly installed on the top cover (801).

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