A compound microbial inoculant for preparing silage feed and a feed preparation method

Abstract

The present invention discloses a compound microbial agent for preparing silage feed and a feed preparation method, belonging to the technical field of fermented feed. The compound microbial agent for preparing silage feed includes one or more of Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens, and Bacillus subtilis. In the present invention, Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens, and Bacillus subtilis are used as a compound microbial agent for feed made from silage corn straw, peanut vine, rice husk, and soybean meal, improving the content of organic matter and water-soluble carbohydrates in the prepared silage feed, reducing the content of cellulose therein, and enhancing the palatability of the silage feed.

Description

Technical Field

[0001] The present invention belongs to the technical field of fermented feeds, and particularly relates to a compound microbial inoculant for preparing silage and a feed preparation method. Background Art

[0002] With the continuous development of the breeding industry, the demand in the feed market continues to grow. The demand for feeds in the breeding industries such as pigs, poultry, and aquaculture is constantly rising. At the same time, the demand for high-quality, green and healthy foods is increasing, making it urgent to expand the high-quality green feed market. As an important part of the diet of ruminants in production, the quality of roughage directly affects the growth performance of animals. Silage is one of the effective methods to improve the nutritional value of straw feeds. Due to its good palatability, rich nutrition, long-term preservation, high digestibility, and low price, silage is widely used in the breeding industry.

[0003] In recent years, sweet corn, as a fresh food corn, is popular among people. Its demand is increasing, and the planting area is gradually expanding. Because of its short growth period and early harvesting, it is selected as the raw material for silage, with good palatability and appropriate water content. In addition, its purchase price is relatively low, which can greatly reduce the breeding cost. However, the crude fiber content in the silage finished product prepared only from sweet corn straw as the raw material for silage is relatively high, and the crude protein content is relatively low. Similarly, peanut vine is a by-product of the important oil crop peanut in China. It has a high protein content but a low sugar content. It is often sun-dried and fed in an air-dried form. However, affected by factors such as weather, it is prone to mildew during sun-drying, resulting in nutrient loss and resource waste. Rice husk powder accounts for more than 20% of the weight of rice and is a common unconventional feed resource. However, about 63.67% of the substances in rice husk powder are cellulose-like substances. The high lignocellulose affects the digestion and absorption of other nutrients by the body. When only using rice husk powder as feed, the digestibility of the feed by animals is only 5-8%. Its fiber content is too high and its palatability is poor, so it is less used in livestock production. The process of fermenting and producing feed can not only convert macromolecular substances in the feed into small molecules, but also adjust the composition of nutrients in the raw materials. The change in the composition of nutrients during the fermentation process is mainly due to the degradation of the substrate and the generation of metabolites by different types of bacteria during their growth. How to adjust the types of fermentation bacteria in combination with the fermentation raw materials to prepare a silage fermentation method suitable for the corresponding raw materials is the key to the preparation of silage. There is no silage preparation method and related feed finished products in the prior art that can improve the crude protein content of silage. Summary of the Invention

[0004] To solve the above technical problems, the present invention provides a composite microbial inoculant for preparing silage feed and a feed preparation method. Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens, and Bacillus subtilis are used as the composite inoculant for the feed made from silage corn straw, peanut vines, rice husks, and soybean meal, which improves the organic matter and water-soluble carbohydrate contents of the prepared silage feed, reduces the cellulose content therein, and enhances the palatability of the silage feed.

[0005] To achieve the above object, the present invention provides a composite microbial inoculant for preparing silage feed, including one or more of Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens, and Bacillus subtilis.

[0006] Preferably, the mass ratio of Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens, and Bacillus subtilis in the composite microbial inoculant is 2-4:0.5-0.8:1-3:2-4:0.5-1.5.

[0007] Preferably, the effective viable count of Lactobacillus rhamnosus is 0.5×10 12 -1.5×10 12 CFU / g, the effective viable count of Saccharomyces cerevisiae is 2×10 10 -4×10 10 CFU / g, the effective viable count of Neurospora crassa is 0.5×10 9 -1.5×10 9 CFU / g, the effective viable count of Bacillus amyloliquefaciens is 0.5×10 9 -1.5×10 9 CFU / g, and the effective viable count of Bacillus subtilis is 1×10 8 -2×10 8 CFU / g.

[0008] The present invention also provides the application of the composite microbial inoculant in the fermentation preparation of corn straw silage feed.

[0009] The present invention also provides a method for preparing silage feed using the composite inoculant, including the following steps:

[0010] (1) Cut sweet corn straw and peanut vines, and mix them with rice husk powder and soybean meal to obtain a raw material mixture;

[0011] (2) Mix the raw material mixture in step (1) with betaine hydrochloride, cellulase, and the composite microbial inoculant, seal it, and perform silage treatment at 25-40°C for 49-56 days to obtain the silage feed.

[0012] Preferably, the mass ratio of the sweet corn straw, peanut vine, rice husk powder, soybean meal, betaine hydrochloride and cellulase is 270-370:270-370:235-245:110-120:7.75-1.5:0.5-1.2.

[0013] Preferably, the length of the shearing in step (1) is 1-2 cm.

[0014] Preferably, the water content of the raw material mixture in step (1) is 60-70%.

[0015] Preferably, the dosage of the compound microbial inoculum in step (2) is 6-10% of the total mass of the raw material mixture, and the silage treatment in step (2) is carried out under light-proof conditions.

[0016] The present invention also provides the corn straw silage prepared by the preparation method.

[0017] Compared with the prior art, the present invention has the following advantages and technical effects:

[0018] The present invention provides a compound microbial inoculum for preparing silage, which is used for silage of feed with corn straw, peanut vine, rice husk and soybean meal as raw materials. Using Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens and Bacillus subtilis as the compound inoculum, making full use of the synergistic fermentation effect of each strain, relatively increasing the content of organic matter and water-soluble carbohydrates in the prepared silage, reducing the content of cellulose therein, and improving the palatability of the silage. Saccharomyces cerevisiae is used to quickly consume the air in the fermentation process, so that the feed can enter the anaerobic fermentation stage faster, accelerating the growth and metabolism speed of other types of bacteria. Under sufficient fermentation conditions, Neurospora crassa can significantly change the composition and content of nutritional components and functional components. Various strains are compounded and fully fermented, and the composition of nutritional components in the feed is adjusted by using enzymes such as α-amylase, protease, lipase and cellulase synthesized by themselves, inhibiting the growth of other pathogenic bacteria and improving the quality of the feed. The present invention adds betaine hydrochloride to the prepared feed to improve the relevant index content, which is helpful for the protein and fat metabolism of feeding animals. Description of the Drawings

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0020] Figure 1 It is a statistical chart of lactic acid content after fermentation of experimental groups 1-5 and control groups 1-5;

[0021] Figure 2 Statistical chart of acetic acid content after fermentation for experimental groups 1 - 5 and control groups 1 - 5. Detailed implementation manners

[0022] The various exemplary implementation manners of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention.

[0023] It should be understood that the terms used in the present invention are only for describing specific implementation manners and are not used to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0024] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0025] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific implementation manners of the present invention specification, which are obvious to those skilled in the art. Other implementation manners obtained from the present invention specification are obvious to those skilled in the art. The present invention specification and examples are merely exemplary.

[0026] Regarding the use of "comprising", "including", "having", "containing", etc. in this article, they are all open-ended terms, meaning including but not limited to.

[0027] The "parts" mentioned in the present invention are all calculated by mass parts unless otherwise specified.

[0028] Sources of experimental supplies used in the present invention: Lactobacillus rhamnosus (HT1) was purchased from the forage processing laboratory of the Department of Grassland Science, South China Agricultural University; Saccharomyces cerevisiae (CICC32336) was purchased from Beijing Yuwei Technology Co., Ltd.; Neurospora crassa (product number: HZB121874) was purchased from Wuhan Huizao Biotechnology; Bacillus amyloliquefaciens (B - 4M - 6) was purchased from Hebei Weierli Animal Pharmaceutical Group; Bacillus subtilis (BS12) was purchased from Harbin Boshan Biology.

[0029] Example 1

[0030] Compound microbial inoculum: Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens and Bacillus subtilis are mixed according to a mass ratio of 3:0.6:2:3:1. The effective viable count of Lactobacillus rhamnosus is 1×10 12 CFU / g, the effective viable count of Saccharomyces cerevisiae is 3×10 10 CFU / g, the effective viable count of Neurospora crassa is 1×10 9 CFU / g, the effective viable count of Bacillus amyloliquefaciens is 1×10 9 CFU / g, the effective viable count of Bacillus subtilis is 1.5×10 8 CFU / g.

[0031] 320 parts of sweet corn straw and 320 parts of peanut vine are cut into small sections with a length of 1.5 cm, mixed with 240 parts of rice husk powder and 117.25 parts of soybean meal, and the moisture content of the raw material mixture is adjusted to 65%; the raw material mixture is mixed with 8% by mass of the compound microbial inoculum based on the mass of the raw material mixture, 1.25 parts of betaine hydrochloride and 1 part of cellulase, sealed, and subjected to anaerobic silage treatment at 28°C in the dark for 52 d to obtain silage feed.

[0032] Example 2

[0033] Compound microbial inoculum: Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens and Bacillus subtilis are mixed according to a mass ratio of 2:0.8:3:4:1.5. The effective viable count of Lactobacillus rhamnosus is 1.5×10 12 CFU / g, the effective viable count of Saccharomyces cerevisiae is 2×10 10 CFU / g, the effective viable count of Neurospora crassa is 0.5×10 9 CFU / g, the effective viable count of Bacillus amyloliquefaciens is 0.5×10 9 CFU / g, the effective viable count of Bacillus subtilis is 1×10 8 CFU / g.

[0034] 270 parts of sweet corn straw and 270 parts of peanut vine are cut into small sections with a length of 2 cm, mixed with 245 parts of rice husk powder and 120 parts of soybean meal, and the moisture content of the raw material mixture is adjusted to 60%; the raw material mixture is mixed with 6% by mass of the compound microbial inoculum based on the mass of the raw material mixture, 0.75 parts of betaine hydrochloride and 0.5 parts of cellulase, sealed, and subjected to anaerobic silage treatment at 25°C in the dark for 56 d to obtain silage feed.

[0035] Example 3

[0036] Compound microbial inoculant: Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens and Bacillus subtilis are mixed according to a mass ratio of 4:0.5:1:2:0.5. The effective viable count of Lactobacillus rhamnosus is 0.5×10 12 CFU / g, the effective viable count of Saccharomyces cerevisiae is 4×10 10 CFU / g, the effective viable count of Neurospora crassa is 1.5×10 9 CFU / g, the effective viable count of Bacillus amyloliquefaciens is 1.5×10 9 CFU / g, the effective viable count of Bacillus subtilis is 2×10 8 CFU / g.

[0037] 370 parts of sweet corn straw and 370 parts of peanut vine are cut into small sections 1 cm long, mixed with 235 parts of rice husk powder and 110 parts of soybean meal, and the moisture content of the raw material mixture is adjusted to 70%; the raw material mixture is mixed with 10% by mass of the compound microbial inoculant based on the mass of the raw material mixture, 1.5 parts of betaine hydrochloride and 1.2 parts of cellulase, sealed, and subjected to dark silage treatment at 40 °C for 49 d to obtain silage.

[0038] Example 4

[0039] Compound microbial inoculant: Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens and Bacillus subtilis are mixed according to a mass ratio of 3.5:0.7:2.1:2.8:0.7. The effective viable count of Lactobacillus rhamnosus is 1×10 12 CFU / g, the effective viable count of Saccharomyces cerevisiae is 3×10 10 CFU / g, the effective viable count of Neurospora crassa is 1×10 9 CFU / g, the effective viable count of Bacillus amyloliquefaciens is 1×10 9 CFU / g, the effective viable count of Bacillus subtilis is 1.5×10 8 CFU / g.

[0040] 300 parts of sweet corn straw and 300 parts of peanut vine are cut into small sections 1 cm long, mixed with 240 parts of rice husk powder and 115 parts of soybean meal, and the moisture content of the raw material mixture is adjusted to 65%; the raw material mixture is mixed with 9% by mass of the compound microbial inoculant based on the mass of the raw material mixture, 1 part of betaine hydrochloride and 0.8 part of cellulase, sealed, and subjected to dark silage treatment at 32 °C for 50 d to obtain silage.

[0041] Example 5

[0042] Compound microbial inoculant: Lactobacillus rhamnosus, Saccharomyces cerevisiae, Neurospora crassa, Bacillus amyloliquefaciens and Bacillus subtilis are mixed in a mass ratio of 2:0.8:1:4:0.5. The effective viable count of Lactobacillus rhamnosus is 1×10 12 CFU / g, the effective viable count of Saccharomyces cerevisiae is 3×10 10 CFU / g, the effective viable count of Neurospora crassa is 1×10 9 CFU / g, the effective viable count of Bacillus amyloliquefaciens is 1×10 9 CFU / g, the effective viable count of Bacillus subtilis is 1.5×10 8 CFU / g.

[0043] 350 parts of sweet corn straw and 350 parts of peanut vine are cut into small sections 1 cm long, mixed with 240 parts of rice husk powder and 110 parts of soybean meal, and the water content of the raw material mixture is adjusted to 65%; the raw material mixture is mixed with 8% by mass of the compound microbial inoculant, 0.9 part of betaine hydrochloride and 0.6 part of cellulase based on the mass of the raw material mixture, sealed, and subjected to anaerobic silage treatment at 30°C in the dark for 54 d to obtain silage.

[0044] Control 1

[0045] 320 parts of sweet corn straw and 320 parts of peanut vine are cut into small sections 1.5 cm long, mixed with 240 parts of rice husk powder and 117.25 parts of soybean meal, and the water content of the raw material mixture is adjusted to 65%; the raw material mixture is mixed with 8% by mass of normal saline, 1.25 parts of betaine hydrochloride and 1 part of cellulase based on the mass of the raw material mixture, sealed, and subjected to anaerobic silage treatment at 28°C in the dark for 52 d to obtain silage.

[0046] Control 2

[0047] 270 parts of sweet corn straw and 270 parts of peanut vine are cut into small sections 2 cm long, mixed with 245 parts of rice husk powder and 120 parts of soybean meal, and the water content of the raw material mixture is adjusted to 60%; the raw material mixture is mixed with 6% by mass of normal saline, 0.75 part of betaine hydrochloride and 0.5 part of cellulase based on the mass of the raw material mixture, sealed, and subjected to anaerobic silage treatment at 25°C in the dark for 56 d to obtain silage.

[0048] Control 3

[0049] 370 parts of sweet corn straw and 370 parts of peanut vine are cut into small sections 1 cm long, mixed with 235 parts of rice husk powder and 110 parts of soybean meal, and the water content of the raw material mixture is adjusted to 70%; the raw material mixture is mixed with 10% by mass of normal saline, 1.5 parts of betaine hydrochloride and 1.2 parts of cellulase based on the mass of the raw material mixture, sealed, and subjected to anaerobic silage treatment at 40°C in the dark for 49 d to obtain silage.

[0050] Control 4

[0051] 300 parts of sweet corn straw and 300 parts of peanut vine are cut into small sections 1 cm long, mixed with 240 parts of rice husk powder and 115 parts of soybean meal, and the water content of the raw material mixture is adjusted to 65%; the raw material mixture is mixed with 9% by mass of the raw material mixture of normal saline, 1 part of betaine hydrochloride and 0.8 part of cellulase, sealed, and subjected to dark silage treatment at 32 °C for 50 d to obtain silage.

[0052] Control Example 5

[0053] 350 parts of sweet corn straw and 350 parts of peanut vine are cut into small sections 1 cm long, mixed with 240 parts of rice husk powder and 110 parts of soybean meal, and the water content of the raw material mixture is adjusted to 65%; the raw material mixture is mixed with 8% by mass of the raw material mixture of normal saline, 0.9 part of betaine hydrochloride and 0.6 part of cellulase, sealed, and subjected to dark silage treatment at 30 °C for 54 d to obtain silage.

[0054] Experimental Example

[0055] Samples of the silages prepared in Control Examples 1 to 5 are taken as Control Groups 1 to 5 respectively, and samples of the silages prepared in Examples 1 to 5 are taken as Experimental Groups 1 to 5 respectively. The crude ash (CA) is determined by the ashing method at 550 °C, and then the organic matter (OM) of the sample is determined by calculation according to the formula OM = DM - CA. The crude protein (CP) is determined by the Kjeldahl method, and the crude fat (EE) is determined by the ether extraction method; the neutral detergent fiber (NDF) and acid detergent fiber (ADF) are determined by the Van Soest fiber analysis method; the ammonia nitrogen (NH3-N) content is determined by the phenol-sodium hypochlorite colorimetric method; the water-soluble carbohydrate (WSC) content is determined by the anthrone-sulfuric acid colorimetric method.

[0056] 40 g of each sample of the silages prepared in Examples 1 to 5 are taken as Experimental Groups 1 to 5 respectively, and samples of the silages prepared in Control Examples 1 to 5 are taken as Control Groups 1 to 5 respectively. 20 g of the sample is mixed with 180 mL of distilled water, homogenized for 60 s, filtered, and the pH value of each group is measured. 20 g of the sample is mixed with 180 mL of deionized water, homogenized for 60 s, and the liquid of each group is filtered and retained. The lactic acid content is determined by the p-hydroxydiphenyl method, and the volatile fatty acid (VFA) content, including acetic acid, propionic acid and butyric acid contents, is determined by high performance gas chromatography.

[0057] Table 1 Determination results of organic matter (OM), crude protein (CP) and crude fat (EE) contents of samples in Experimental Groups 1 to 5 and Control Groups 1 to 5

[0058] Group Organic matter OM(%) Crude protein CP(%) Crude fat EE(%) Control group 1 85.12 13.75 3.57 Experimental group 1 90.29 13.66 3.49 Control group 2 85.01 13.12 3.02 Experimental group 2 89.25 12.83 2.97 Control group 3 86.24 13.87 3.89 Experimental group 3 89.11 13.52 3.74 Control group 4 86.55 13.62 3.63 Experimental group 4 88.95 12.98 3.27 Control group 5 86.37 12.96 3.25 Experimental group 5 88.74 12.08 3.01

[0059] Determination Results of Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) Contents in Samples of Experimental Groups 1 - 5 and Control Groups 1 - 5 in Table 2

[0060]

[0061]

[0062] Table 3 Determination Results of Ammonia Nitrogen (NH3-N), Ammonia Nitrogen / Total Nitrogen (NH3-N / TN) and Water-Soluble Carbohydrates (WSC) Contents in Samples of Experimental Groups 1 - 5 and Control Groups 1 - 5

[0063]

[0064] Table 4 Determination Results of pH, Lactic Acid, Acetic Acid, Propionic Acid and Butyric Acid Contents in Samples of Experimental Groups 1 - 5 and Control Groups 1 - 5

[0065]

[0066]

[0067] It can be seen from the results recorded in Tables 1 - 3 that the contents of organic matter OM and water-soluble carbohydrates WSC in Experimental Groups 1 - 5 treated with the compound microbial inoculant and silage method described in the present invention are increased compared with those in the corresponding treatment groups, Control Groups 1 - 5, while the contents of neutral detergent fiber NDF, acid detergent fiber ADF and ammonia nitrogen NH3-N are significantly reduced compared with those in the corresponding treatment groups, Control Groups 1 - 5. There is basically no influence on the contents of crude protein CP and crude fat EE. When the fiber content is reduced, the contents of crude protein and fat in the feed are relatively increased.

[0068] It can be seen from the results recorded in Table 4 and Figure 1-2 that after fermentation, Experimental Groups 1 - 5 treated with the compound microbial inoculant and silage method described in the present invention have a significant influence on the contents of lactic acid and acetic acid compared with the corresponding treatment groups, Control Groups 1 - 5.

[0069] The compound microbial inoculant and silage method described in the present invention improve the quality of the silage prepared with sweet corn stover and peanut vine in the same proportion as raw materials, and adjust the composition of the nutritional components in the fermented feed.

[0070] The above-described embodiments are only descriptions of the preferred modes of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A compound microbial inoculant for preparing silage, characterized in that, It consists of Lactobacillus rhamnosus HT1, Saccharomyces cerevisiae CICC3236, Neurospora crassa HZB121874, Bacillus amyloliquefaciens B-4M-6 and Bacillus subtilis BS12.

2. The compound microbial inoculum according to claim 1, wherein In the compound microbial inoculum, the mass ratio of Lactobacillus rhamnosus HT1, Saccharomyces cerevisiae CICC3236, Neurospora crassa HZB121874, Bacillus amyloliquefaciens B-4M-6 and Bacillus subtilis BS12 is 2 - 4:0.5 - 0.8:1 - 3:2 - 4:0.5 - 1.

5.

3. The composite microbial inoculum according to claim 1, wherein The viable count of Lactobacillus rhamnosus HT1 is 0.5×10 12 ~1.5×10 12 CFU / g, the viable count of Saccharomyces cerevisiae CICC3236 is 2×10 10 ~4×10 10 CFU / g, the viable count of Neurospora crassa HZB121874 is 0.5×10 9 ~1.5×10 9 CFU / g, the viable count of Bacillus amyloliquefaciens B-4M-6 is 0.5×10 9 ~1.5×10 9 CFU / g, the viable count of Bacillus subtilis BS12 is 1×10 8 ~2×10 8 CFU / g.

4. Use of the compound microbial inoculum according to any one of claims 1 to 3 in the fermentation preparation of corn straw silage feed.

5. A method for preparing silage using the composite microbial agent according to any one of claims 1 to 3, characterized in that, It includes the following steps: (1) Cut sweet corn straws and peanut vines, and mix them with rice husk powder and soybean meal to obtain a raw material mixture. (2) Mix the raw material mixture described in step (1) with betaine hydrochloride, cellulase and the compound microbial inoculum, seal it, and perform silage treatment at 25 - 40 °C for 49 - 56 d to obtain silage feed.

6. The method for preparing silage according to claim 5, characterized in that, The mass ratio of the sweet corn straws, peanut vines, rice husk powder, soybean meal, betaine hydrochloride and cellulase is 270 - 370:270 - 370:235 - 245:110 - 120:7.75 - 1.5:0.5 - 1.

2.

7. The method for preparing silage according to claim 5, wherein The length of the cutting described in step (1) is 1 - 2 cm.

8. The method for preparing silage according to claim 5, characterized in that, The water content of the raw material mixture described in step (1) is 60 - 70%.

9. The method for preparing silage according to claim 5, characterized in that, The dosage of the compound microbial inoculum in step (2) is 6 - 10% of the total mass of the raw material mixture, and the silage treatment in step (2) is carried out under light - avoiding conditions.

10. Corn straw silage feed prepared by the preparation method according to any one of claims 5 to 9.

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