A compound carbon source preparation device and method based on gene sequencing

Abstract

The present invention discloses a device and method for preparing a composite carbon source based on gene sequencing, aiming to solve the deficiencies that the preparation of the composite carbon source is inconvenient and it is difficult to achieve the optimal treatment effect for the prepared composite carbon source. The invention includes a main preparation unit and a trial preparation unit. The trial preparation unit is detachably installed on the main preparation unit. An active stirring component is arranged in the main preparation unit, and a driven stirring component is arranged in the trial preparation unit. The active stirring component can drive the driven stirring component to rotate. The device for preparing the composite carbon source facilitates the trial preparation and large-scale preparation of the composite carbon source. The composite carbon source is first subjected to trial preparation, and after achieving the expected effect, large-scale preparation is carried out, so as to obtain a composite carbon source finished product that meets the expectations, with high production efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of carbon source preparation, and more specifically, it relates to a device and method for preparing a composite carbon source based on gene sequencing. Background Art

[0002] A carbon source is a carbon-containing compound that can provide nutrients for the growth and metabolism of microorganisms in a sewage biochemical treatment system. Carbon sources are divided into single carbon sources and composite carbon sources. A single carbon source is a carbon source that contains only one effective carbon source component, and a composite carbon source is composed of two or more effective carbon source components. The composite carbon source can overcome the disadvantages of traditional carbon source products, such as a large dosage, difficult to dissolve at low temperatures, crystallization, solidification, low microbial absorption conversion rate, poor total nitrogen removal rate, high carbon source hazard, and strong pungent smell. Moreover, the composite carbon source has a high safety factor, is not a chemical waste, is non-corrosive, and will not introduce new pollutants. The composite carbon source has a high concentration, saves freight, can reduce the production of organic sludge, and improve the sludge activity. Therefore, the composite carbon source has been widely used.

[0003] However, at present, the composite carbon source market is relatively complex, and the use effects are uneven. This is mainly because the content of bacteria in different polluted water bodies is different. If a suitable composite carbon source is not selected according to the specific situation of the mixed bacteria, it is difficult to achieve the optimal treatment effect. Summary of the Invention

[0004] In order to overcome the above deficiencies, the present invention provides a device and method for preparing a composite carbon source based on gene sequencing. The device for preparing the composite carbon source facilitates the trial preparation and large-scale preparation of the composite carbon source. The composite carbon source is first subjected to trial preparation, and after achieving the expected effect, large-scale preparation is carried out, thereby obtaining a composite carbon source finished product that meets the expectations, with high production efficiency.

[0005] To solve the above technical problems, the present invention adopts the following technical solution: A device for preparing a composite carbon source based on gene sequencing, including a main preparation unit and a trial preparation unit. The trial preparation unit is detachably installed on the main preparation unit. An active stirring component is arranged in the main preparation unit, and a driven stirring component is arranged in the trial preparation unit. The active stirring component can drive the driven stirring component to rotate.

[0006] The composite carbon source preparation device of the present application can achieve small-scale pre-preparation and large-scale mass production configuration of carbon sources. During small-scale pre-preparation, the carbon source raw materials are loaded into the trial preparation unit, and the active stirring component operates and rotates to drive the driven stirring component to rotate, so that the carbon source raw materials are evenly mixed. The trial preparation unit is disassembled from the main preparation unit, and the pre-prepared composite carbon source is put into the collected sewage sample for use, and whether the use effect meets the expectation is observed. If the use effect does not meet the expectation, the carbon source raw materials and their ratios are adjusted and re-prepared until the use effect meets the expectation. Referring to the raw materials and ratios required for the composite carbon source with the expected use effect, the main preparation unit is used for large-scale preparation, and the active stirring component operates and rotates to stir and mix the raw materials to obtain the finished composite carbon source that meets the expectation.

[0007] The composite carbon source preparation device facilitates the trial preparation and large-scale preparation of composite carbon sources. The composite carbon source is first subjected to trial preparation, and after reaching the expected effect, large-scale preparation is carried out, so as to obtain the finished composite carbon source that meets the expectation, with high production efficiency.

[0008] Preferably, a feeding hopper is arranged on the main preparation unit, and the trial preparation unit includes a trial preparation cylinder. The trial preparation cylinder is hermetically sleeved and connected to the lower end of the feeding hopper, and a feeding port is arranged on the trial preparation cylinder.

[0009] The arrangement of the feeding hopper facilitates the flow of carbon source raw materials. During pre-preparation, the trial preparation cylinder is hermetically sleeved and connected to the lower end of the feeding hopper, and the feeding port is exposed in the feeding hopper. At this time, the carbon source raw materials flow into the trial preparation cylinder through the feeding port.

[0010] Preferably, the trial preparation unit is connected to a support plate, and a piston cylinder is installed on the main preparation unit. The telescopic rod of the piston cylinder pushes and pulls the support plate to realize the movement of the trial preparation unit.

[0011] The trial preparation unit realizes lifting movement through the drive of the piston cylinder, which can ensure the accuracy of the moving position. The support plate serves as the stress point for pushing and pulling movement, and the support plate can be disengaged from the telescopic rod of the piston cylinder, which is convenient for disassembling the trial preparation unit from the main preparation unit.

[0012] Preferably, an upper cover is arranged on the upper part of the main preparation unit, and an avoidance notch for the support plate to pass through is arranged on the upper cover. The trial preparation unit rotates to make the support plate correspond and fit with the avoidance notch or be misaligned and separated, and the trial preparation unit rotates to make the telescopic rod of the piston cylinder be misaligned with the support plate.

[0013] During the process of installing the trial preparation unit onto the main preparation unit, first align the support plate with the avoidance notch, insert the entire trial preparation unit downward into the main preparation unit. After inserting a certain depth, rotate the trial preparation unit to make the support plate be misaligned and separated from the avoidance notch, and align the support plate with the telescopic rod of the piston cylinder. At this time, the piston cylinder can push and pull the support plate to lift and move.

[0014] When the trial - matching unit is disassembled from the main - matching unit, first rotate the trial - matching unit to misalign the piston - cylinder telescopic rod with the support plate, align the support plate with the avoidance notch, and then the entire trial - matching unit can be pulled outwards from the main - matching unit.

[0015] Preferably, two piston - cylinders are provided. Limit plates are connected to the two piston - cylinder telescopic rods. One limit plate is placed below the support plate, and the other limit plate is placed above the support plate. The support plate moves within the range between the two limit plates.

[0016] By providing two limit plates to limit the support plate between the two limit plates, it is ensured that the trial - matching cylinder is always in a sealed sleeve with the material - guiding hopper, preventing the carbon - source raw materials from flowing into the preparation tank of the main - matching unit during the trial - matching process.

[0017] Preferably, the main - matching unit includes a preparation tank. The active stirring assembly includes a stirring shaft, which is rotatably installed in the preparation tank, and several stirring rods are installed on the stirring shaft.

[0018] When preparing a large - scale production of composite carbon source, first disassemble the trial - matching unit, let the carbon - source raw materials flow into the preparation tank through the material - guiding hopper, and rotate the stirring shaft to stir the mixed liquid evenly for the preparation of the composite carbon source.

[0019] Preferably, the driven stirring assembly includes a rotating shaft, and several magnetic stirring blades are circumferentially spaced on the rotating shaft. Several magnets are installed at the upper end of the active stirring assembly at circumferential intervals. The magnets and the magnetic stirring blades attract each other, and the magnets rotate with the active stirring assembly to drive the magnetic stirring blades to rotate.

[0020] Through the mutual attraction between the magnets and the magnetic stirring blades, the active stirring assembly can drive the magnetic stirring blades to rotate during rotation. This non - contact transmission method is convenient for layout.

[0021] In another solution, the driven stirring assembly includes a rotating shaft, a stirring blade is installed on the upper part of the rotating shaft, a turntable is arranged at the lower part of the rotating shaft, a slot is arranged on the turntable, and a plug rod is arranged on the active stirring assembly. The plug rod is inserted into the slot so that the active stirring assembly drives the rotating shaft to rotate.

[0022] After the trial - matching unit is installed into the main - matching unit, the slot on the turntable is correspondingly inserted with the plug rod, so that the circumferential direction between the plug rod and the slot can be limited. During the rotation of the active stirring assembly, the turntable can be driven to rotate. This transmission method is stable and reliable.

[0023] Preferably, a sealing ring is movably sleeved in the trial - matching cylinder. A slot hole corresponding to the feeding port is arranged on the sealing ring, a dial rod protruding outwards is arranged on the sealing ring, and a contact block is arranged in the material - guiding hopper; when the trial - matching cylinder rotates, the dial rod abuts against the contact block, so that the sealing ring and the trial - matching cylinder rotate relative to each other, and the sealing ring closes the feeding port.

[0024] Before the trial - matching unit is removed from the main - matching unit, first rotate the trial - matching cylinder. The lever abuts against the abutting block, so that the sealing ring and the trial - matching cylinder rotate relative to each other, and the sealing ring closes the feeding port. During this process, the support plate first separates from the piston - cylinder telescopic rod, then the support plate aligns with the avoidance notch, and finally the entire trial - matching cylinder can be pulled out upward. The sealing ring closes the feeding port, preventing the composite carbon source from flowing out of the feeding port during the removal of the trial - matching cylinder. When pouring the composite carbon source in the trial - matching cylinder into the sampled sewage, it also prevents the composite carbon source from flowing away from the feeding port.

[0025] A method for preparing a composite carbon source based on gene sequencing, which is prepared by using a composite - carbon - source preparation device based on gene sequencing, includes the following steps: S1, collecting sewage samples, selecting sewage at different depth positions and filling them in different samplers respectively; S2, performing gene sequencing on the strains in the sewage samples in the samplers to obtain the distribution information of the strains in the sewage; S3, selecting appropriate carbon - source raw materials for preparation according to the obtained strain distribution information; during preparation, first perform small - scale pre - preparation, load the carbon - source raw materials into the trial - matching unit, and the active stirring component runs and rotates to drive the driven stirring component to rotate; S4, putting the pre - prepared composite carbon source into the collected sewage samples for use; S5, observing whether the use effect meets the expectation. If the use effect does not meet the expectation, repeat S3 and S4, adjust the carbon - source raw materials and their ratios until the use effect meets the expectation; S6, referring to the raw materials and ratios required for the composite carbon source with the expected use effect, use the main - matching unit for large - scale preparation, and the active stirring component runs and rotates to stir and mix the raw materials to obtain the composite - carbon - source finished product that meets the expectation.

[0026] In the present invention, after scientifically sampling the sewage water body at multiple levels, then performing gene sequencing on the water - body sample through the gene - sequencing module to obtain the distribution information of the strains in the water body. According to the obtained strain distribution information, reasonably select carbon - source raw materials for scientific preparation. Before preparation, a small - scale pre - configuration is carried out through the trial - matching unit. After obtaining the finished product, it is put into the sample taken by the sampling module for use again until the use effect meets the expectation. Then, through the main - matching unit, large - scale preparation is carried out to obtain the composite - carbon - source finished product that meets the expectation.

[0027] According to the strain distribution information in the sewage, carbon - source raw materials are selected to prepare the composite carbon source. The prepared composite carbon source has strong pertinence and good use effect. Moreover, in this application, small - scale trial production is carried out first, and the composite carbon source obtained from the trial production is used first to observe the use effect. Until the use effect meets the expectation, large - scale preparation of the composite carbon source is carried out, thus obtaining the final product, with high production efficiency. It avoids the poor use effect of the composite carbon source obtained by directly carrying out large - scale preparation, resulting in waste and affecting production efficiency.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The composite carbon source preparation device facilitates the trial preparation and large-scale preparation of the composite carbon source. The composite carbon source is first prepared through trial preparation, and after achieving the expected effect, large-scale preparation is carried out, thereby obtaining a composite carbon source finished product that meets the expectations, with high production efficiency; (2) According to the information on the distribution of bacteria in the sewage, carbon source raw materials are selected to prepare the composite carbon source, and the prepared composite carbon source has strong pertinence and good use effect; (3) The active stirring component operates and rotates to drive the driven stirring component to rotate, realizing the stirring of the mixed liquid in the trial preparation cylinder, without the need to separately set a driving device for the trial preparation unit, simplifying the structure and reducing the cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is a schematic structural diagram of the present invention.

[0030] Figure 2 is a schematic structural diagram of the present invention in another direction.

[0031] Figure 3 is a schematic structural diagram of the interior of the preparation tank of the present invention.

[0032] Figure 4 is a schematic structural diagram of the connection structure of the trial preparation cylinder of the present invention.

[0033] Figure 5 is a schematic structural diagram of the interior of the trial preparation cylinder of the present invention.

[0034] Figure 6 is a schematic diagram of the driven stirring component of Embodiments 2 and 3 of the present invention.

[0035] Figure 7 is a schematic structural diagram of the connection structure of the closed ring of Embodiment 3 of the present invention.

[0036] In the figures: 100 - main preparation unit, 101 - preparation tank, 101a - avoidance notch, 102 - fixed ring plate, 103 - support leg, 104 - drive motor, 105 - discharge pipe, 106 - control valve, 107 - feed hopper, 108 - stirring shaft, 108a - connecting rod, 108b - mounting plate, 108c - magnetic block, 109 - stirring rod, 110 - controller, 200 - trial preparation unit, 201 - guiding hopper, 202 - piston cylinder, 202a - piston cylinder telescopic rod, 203 - trial preparation cylinder, 203a - feeding port, 204 - connecting rod, 205 - support plate, 206 - sealing cover, 207 - limiting plate, 208 - rotating shaft, 209 - magnetic stirring blade, 210 - stirring blade, 211 - turntable, 212 - slot, 213 - inserting rod, 214 - closed ring, 215 - slot hole, 216 - lever, 217 - abutting block, 218 - sliding groove, 219 - base. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0037] The technical solution of the present invention will be further specifically described below through specific embodiments in conjunction with the accompanying drawings:

[0038] Embodiment 1: A compound carbon source preparation device based on gene sequencing (see attached Figure 1 to attached Figure 5 ), which includes a main preparation unit 100 and a trial preparation unit 200. The trial preparation unit 200 is detachably installed on the main preparation unit 100. The main preparation unit 100 includes a preparation tank 101. The bottom of the preparation tank 101 is in a conical structure with a larger upper part and a smaller lower part. A driving motor 104 is installed at the lower part of the preparation tank 101, and the driving motor 104 is installed outside the preparation tank 101. A fixed ring plate 102 is arranged at a position close to the lower part on the outer wall of the preparation tank 101. A plurality of support legs 103 are circumferentially spaced on the fixed ring plate 102, and the support legs 103 support the whole device. A discharge pipe 105 is connected to the preparation tank 101 near the bottom, and a control valve 106 is installed on the discharge pipe 105. A heater is arranged inside the preparation tank 101, and a controller 110 electrically connected to the heater is installed outside the preparation tank 101.

[0039] An active stirring assembly is arranged in the main preparation unit 100, and a driven stirring assembly is arranged in the trial preparation unit 200. The active stirring assembly can drive the driven stirring assembly to rotate.

[0040] A feed hopper 201 is arranged on the main preparation unit 100. The feed hopper 201 is in a conical structure with a larger upper part and a smaller lower part. The trial preparation unit 200 includes a trial preparation cylinder 203. The trial preparation cylinder 203 is hermetically sleeved and connected to the lower end of the feed hopper 201. A feed inlet 203a is arranged on the trial preparation cylinder 203. The size of the lower end opening of the feed hopper 201 is adapted to the outer diameter of the trial preparation cylinder 203, and the trial preparation cylinder 203 is movably sleeved on the lower end opening of the feed hopper 201. The feed inlet 203a is placed inside the feed hopper 201. The upper end of the trial preparation cylinder 203 is connected to a sealing cover 206.

[0041] The trial preparation unit 200 is connected to a support plate 205. A piston cylinder 202 is installed on the main preparation unit 100. The piston cylinder telescopic rod 202a pushes and pulls the support plate 205 to realize the movement of the trial preparation unit 200. In this embodiment, the piston cylinder 202 uses an air cylinder. An upper cover is arranged at the upper part of the main preparation unit 100. The piston cylinder 202 is installed on the upper cover. An avoidance notch 101a for the support plate 205 to pass through is arranged on the upper cover. The trial preparation unit 200 rotates to make the support plate 205 correspond and fit with or be misaligned and separated from the avoidance notch 101a. The trial preparation unit 200 rotates to make the piston cylinder telescopic rod 202a be misaligned with the support plate 205. Two feed hoppers 107 are arranged on the upper cover, and the two feed hoppers 107 are arranged oppositely.

[0042] There are two piston cylinders 202. Limit plates 207 are connected to the telescopic rods 202a of both piston cylinders. One limit plate 207 is placed below the support plate 205, and the other limit plate 207 is placed above the support plate 205. The support plate 205 moves within the range between the two limit plates 207. In this embodiment, there are two support plates 205. Both support plates 205 are in an arc structure. The two support plates 205 are arranged at intervals in the circumferential direction. The support plate 205 and the trial fitting cylinder 203 are fixedly connected by a connecting rod 108a. One limit plate 207 is placed above one support plate 205, and the other limit plate 207 is placed below the other support plate 205. Two avoidance notches 101a are correspondingly arranged on the upper cover and the two support plates 205. The avoidance notches 101a are arranged at a 90-degree dislocation with the support plate 205. Insertion holes are correspondingly arranged on the upper cover and the trial fitting cylinder 203. The trial fitting cylinder 203 is inserted and connected with the insertion holes. A through groove is arranged between the avoidance notch 101a and the insertion hole, which facilitates the connecting rod 204 to pass through. The width of the limit plate 207 is smaller than the gap between the two support plates 205, which facilitates the limit plate 207 to pass through the gap between the two support plates 205 after the trial fitting cylinder 203 rotates 90 degrees.

[0043] The active stirring assembly includes a stirring shaft 108. The stirring shaft 108 is rotatably installed in the preparation tank 101, and several stirring rods 109 are installed on the stirring shaft 108. The stirring rods 109 are arranged in several circles at intervals up and down, and four are evenly arranged in each circle. The lower end of the stirring shaft 108 extends out of the preparation tank 101 and is connected to the output shaft of the driving motor 104.

[0044] The driven stirring assembly includes a rotating shaft 208. The rotating shaft 208 is rotatably installed at the bottom of the trial fitting cylinder 203. The driven stirring assembly is arranged below the feeding port 203a. Several magnetic stirring blades 209 are arranged at intervals in the circumferential direction on the rotating shaft 208. Several magnetic blocks 108c are installed at intervals in the circumferential direction at the upper end of the active stirring assembly. An installation disk 108b is arranged at the upper end of the stirring shaft 108 of the active stirring assembly. The magnetic blocks 108c are fixedly installed on the installation disk 108b. The lower end of the trial fitting cylinder 203 is arranged close to the installation disk 108b. The installation disk 108b and the stirring shaft 108 are fixedly connected by a connecting rod 108a. The magnetic blocks 108c and the magnetic stirring blades 209 attract each other. The magnetic blocks 108c rotate together with the active stirring assembly, thereby driving the magnetic stirring blades 209 to rotate.

[0045] A method for preparing a composite carbon source based on gene sequencing, which is prepared by using a device for preparing a composite carbon source based on gene sequencing, includes the following steps: S1, collecting sewage samples, selecting sewage at different depth positions, and respectively filling them in different samplers; S2, performing gene sequencing on the bacteria in the sewage samples in the samplers, averaging the number of bacteria measured in the sewage samples taken at different depth positions, calculating the average value of the bacteria per unit volume, and obtaining the distribution information of the bacteria in the sewage; S3, selecting appropriate carbon source raw materials for preparation according to the obtained bacteria distribution information; the types of bacteria measured are Alcaligenes, Neisseriaceae, Rhodospirillaceae, Bacillaceae, Spirillaceae, and the carbon source raw materials with good treatment effects on these types of bacteria are glucose, sodium acetate, propylene glycol, acetic acid, methanol, and starch respectively. When preparing the carbon source raw materials, the quantity ratio of Alcaligenes, Neisseriaceae, Rhodospirillaceae, Bacillaceae, Spirillaceae is a:b:c:d:e, and the weight ratio of the corresponding glucose, sodium acetate, propylene glycol, acetic acid, methanol, and starch is also a:b:c:d:e. When preparing, first perform small-scale pre-preparation, load the carbon source raw materials into the test preparation unit 200, and the active stirring component operates to drive the driven stirring component to rotate; S4, putting the pre-prepared composite carbon source into the collected sewage samples for use; S5, observing whether the use effect meets the expectation, if the use effect does not meet the expectation, repeat S3 and S4, adjust the carbon source raw materials and their ratios until the use effect meets the expectation; S6, referring to the raw materials and ratios required for the composite carbon source with the expected use effect, perform large-scale preparation using the main preparation unit 100, and the active stirring component operates to stir and mix the raw materials to obtain a composite carbon source finished product that meets the expectation.

[0046] After scientifically sampling the sewage water body at multiple levels, the present invention then performs gene sequencing on the water body sample through the gene sequencing module, thereby obtaining the distribution information of the bacteria in the water body. According to the obtained bacteria distribution information, reasonably select carbon source raw materials for scientific preparation. Before preparation, perform small-scale pre-configuration through the test preparation unit 200, put the obtained finished product into the sample taken by the sampling module for use again until the use effect meets the expectation, and then perform large-scale preparation through the main preparation unit 100 to obtain a composite carbon source finished product that meets the expectation.

[0047] According to the bacteria distribution information in the sewage, select carbon source raw materials to prepare a composite carbon source. The prepared composite carbon source has strong pertinence and good use effect. Moreover, in this application, first perform small-scale trial production, first use the composite carbon source obtained from the trial production, observe the use effect, until the use effect meets the expectation, and then perform large-scale preparation of the composite carbon source, thereby obtaining the final product, with high production efficiency. It avoids the poor use effect of the composite carbon source obtained by directly performing large-scale preparation, resulting in waste and affecting production efficiency.

[0048] Embodiment 2: A compound carbon source preparation device based on gene sequencing (see the appendix Figure 6 ), which includes a main preparation unit 100 and a trial preparation unit 200. The trial preparation unit 200 is detachably installed on the main preparation unit 100. The main preparation unit 100 includes a preparation tank 101. The bottom of the preparation tank 101 has a conical structure with a larger upper part and a smaller lower part. A driving motor 104 is installed at the lower part of the preparation tank 101, and the driving motor 104 is installed outside the preparation tank 101. A fixed ring plate 102 is arranged at a position close to the lower part on the outer wall of the preparation tank 101. A number of support legs 103 are circumferentially spaced on the fixed ring plate 102, and the support legs 103 support the whole device. A discharge pipe 105 is connected to the preparation tank 101 at a position close to the bottom, and a control valve 106 is installed on the discharge pipe 105. A heater is arranged inside the preparation tank 101, and a controller 110 electrically connected to the heater is installed outside the preparation tank 101.

[0049] An active stirring assembly is arranged in the main preparation unit 100, and a driven stirring assembly is arranged in the trial preparation unit 200. The active stirring assembly can drive the driven stirring assembly to rotate.

[0050] A feed hopper 201 is arranged on the main preparation unit 100. The feed hopper 201 has a conical structure with a larger upper part and a smaller lower part. The trial preparation unit 200 includes a trial preparation cylinder 203. The trial preparation cylinder 203 is hermetically sleeved and connected to the lower end of the feed hopper 201. A feed inlet 203a is arranged on the trial preparation cylinder 203. The size of the lower end opening of the feed hopper 201 is adapted to the outer diameter of the trial preparation cylinder 203, and the trial preparation cylinder 203 is movably sleeved with the lower end opening of the feed hopper 201. The feed inlet 203a is placed inside the feed hopper 201. The upper end of the trial preparation cylinder 203 is connected to a sealing cover 206.

[0051] The trial preparation unit 200 is connected to a support plate 205. A piston cylinder 202 is installed on the main preparation unit 100. The piston cylinder telescopic rod 202a pushes and pulls the support plate 205 to realize the movement of the trial preparation unit 200. In this embodiment, the piston cylinder 202 adopts a pneumatic cylinder. An upper cover is arranged at the upper part of the main preparation unit 100. The piston cylinder 202 is installed on the upper cover. An avoidance notch 101a for the support plate 205 to pass through is arranged on the upper cover. The trial preparation unit 200 rotates to make the support plate 205 correspond and fit with or be misaligned and separated from the avoidance notch 101a. The trial preparation unit 200 rotates to make the piston cylinder telescopic rod 202a be misaligned with the support plate 205. Two feed hoppers 107 are arranged on the upper cover, and the two feed hoppers 107 are arranged oppositely.

[0052] There are two piston cylinders 202. Limit plates 207 are connected to the telescopic rods 202a of the two piston cylinders. One limit plate 207 is placed below the support plate 205, and the other limit plate 207 is placed above the support plate 205. The support plate 205 moves within the range between the two limit plates 207. In this embodiment, there are two support plates 205, both of which are arc-shaped structures. The two support plates 205 are circumferentially spaced. The support plate 205 and the trial fitting cylinder 203 are fixedly connected by a connecting rod 108a. One limit plate 207 is placed above one support plate 205, and the other limit plate 207 is placed below the other support plate 205. Two avoidance notches 101a are correspondingly arranged on the upper cover and the two support plates 205. The avoidance notches 101a are arranged with a 90-degree offset from the support plate 205. Insertion holes are correspondingly arranged on the upper cover and the trial fitting cylinder 203. The trial fitting cylinder 203 is inserted and connected to the insertion holes. A through groove is arranged between the avoidance notch 101a and the insertion hole, which facilitates the connecting rod 204 to pass through. The width of the limit plate 207 is smaller than the gap between the two support plates 205, which facilitates the limit plate 207 to pass through the gap between the two support plates 205 after the trial fitting cylinder 203 rotates 90 degrees.

[0053] The active stirring assembly includes a stirring shaft 108. The stirring shaft 108 is rotatably installed in the preparation tank 101, and a number of stirring rods 109 are installed on the stirring shaft 108. The stirring rods 109 are arranged in several circles at intervals up and down, and four are evenly distributed in each circle. The lower end of the stirring shaft 108 extends out of the preparation tank 101 and is connected to the output shaft of the driving motor 104.

[0054] The driven stirring assembly includes a rotating shaft 208. The rotating shaft 208 is rotatably installed at the bottom of the trial fitting cylinder 203. The driven stirring assembly is arranged below the feeding port 203a. Stirring blades 210 are installed on the upper part of the rotating shaft 208. A number of stirring blades 210 are circumferentially spaced. A turntable 211 is arranged at the lower part of the rotating shaft 208. The turntable 211 is placed outside the trial fitting cylinder 203. Two slots 212 are arranged on the turntable 211. Two insertion rods 213 are arranged on the active stirring assembly. The insertion rods 213 are arranged on the mounting plate 108b. The size of the slots 212 is larger than the outer diameter of the insertion rods 213. The insertion rods 213 are inserted into the slots 212 so that the active stirring assembly drives the rotating shaft 208 to rotate. The stirring shaft 108 and the rotating shaft 208 are coaxially arranged, and the insertion rods 213 deviate from the axis of the stirring shaft 108. The lower end of the trial fitting cylinder 203 is detachably connected to the base 219, and the rotating shaft 208 is rotatably installed on the base 219.

[0055] A method for preparing a composite carbon source based on gene sequencing, which is prepared by using a device for preparing a composite carbon source based on gene sequencing, includes the following steps: S1, collecting sewage samples, selecting sewage at different depth positions, and respectively filling them into different samplers; S2, performing gene sequencing on the bacteria in the sewage samples in the samplers, averaging the number of bacteria measured in the sewage samples taken at different depth positions, calculating the average value of the bacteria per unit volume, and obtaining the distribution information of the bacteria in the sewage; S3, selecting appropriate carbon source raw materials for preparation according to the obtained bacteria distribution information; the types of bacteria measured are Alcaligenes, Neisseriaceae, Rhodospirillaceae, Bacillaceae, Spirillaceae, and the carbon source raw materials with good treatment effects on these types of bacteria are glucose, sodium acetate, propylene glycol, acetic acid, methanol, and starch respectively. When preparing the carbon source raw materials, the quantity ratio of Alcaligenes, Neisseriaceae, Rhodospirillaceae, Bacillaceae, Spirillaceae is a:b:c:d:e, and the weight ratio of the corresponding glucose, sodium acetate, propylene glycol, acetic acid, methanol, and starch is also a:b:c:d:e. When preparing, first perform small-scale pre-preparation, load the carbon source raw materials into the test preparation unit 200, and the active stirring component operates to drive the driven stirring component to rotate; S4, putting the pre-prepared composite carbon source into the collected sewage samples for use; S5, observing whether the use effect meets the expectation, if the use effect does not meet the expectation, repeat S3 and S4, adjust the carbon source raw materials and their ratios until the use effect meets the expectation; S6, referring to the raw materials and ratios required for the composite carbon source with the expected use effect, perform large-scale preparation using the main preparation unit 100, and the active stirring component operates to stir and mix the raw materials to obtain a composite carbon source finished product that meets the expectation.

[0056] After scientifically sampling the sewage water body at multiple levels in the present invention, then performing gene sequencing on the water body sample through the gene sequencing module to obtain the distribution information of the bacteria in the water body, and reasonably selecting carbon source raw materials for scientific preparation according to the obtained bacteria distribution information. Before preparation, small-scale pre-configuration is carried out through the test preparation unit 200, and after obtaining the finished product, it is put into the sample taken by the sampling module for use again until the use effect meets the expectation, and then large-scale preparation is carried out through the main preparation unit 100 to obtain a composite carbon source finished product that meets the expectation.

[0057] According to the bacteria distribution information in the sewage, carbon source raw materials are selected to prepare the composite carbon source. The prepared composite carbon source has strong pertinence and good use effect. Moreover, in this application, small-scale trial production is carried out first, the composite carbon source obtained from the trial production is used first, and the use effect is observed until the use effect meets the expectation, and then large-scale preparation of the composite carbon source is carried out to obtain the final product, with high production efficiency. It avoids the poor use effect of the composite carbon source obtained by directly carrying out large-scale preparation, resulting in waste and affecting production efficiency.

[0058] Embodiment 3: A compound carbon source preparation device based on gene sequencing (see attached Figure 6 , attached Figure 7 ), which includes a main preparation unit 100 and a trial preparation unit 200. The trial preparation unit 200 is detachably installed on the main preparation unit 100. The main preparation unit 100 includes a preparation tank 101. The bottom of the preparation tank 101 is in a conical structure with a larger upper part and a smaller lower part. A driving motor 104 is installed at the lower part of the preparation tank 101, and the driving motor 104 is installed outside the preparation tank 101. A fixed ring plate 102 is arranged at a position close to the lower part on the outer wall of the preparation tank 101. A number of support legs 103 are circumferentially spaced on the fixed ring plate 102, and the support legs 103 support the whole device. A discharge pipe 105 is connected to the preparation tank 101 at a position close to the bottom, and a control valve 106 is installed on the discharge pipe 105. A heater is arranged inside the preparation tank 101, and a controller 110 electrically connected to the heater is installed outside the preparation tank 101.

[0059] An active stirring assembly is arranged in the main preparation unit 100, and a driven stirring assembly is arranged in the trial preparation unit 200. The active stirring assembly can drive the driven stirring assembly to rotate.

[0060] A material guiding hopper 201 is arranged on the main preparation unit 100. The material guiding hopper 201 is in a conical structure with a larger upper part and a smaller lower part. The trial preparation unit 200 includes a trial preparation cylinder 203. The trial preparation cylinder 203 is hermetically sleeved and connected to the lower end of the material guiding hopper 201. A feed inlet 203a is arranged on the trial preparation cylinder 203. The opening size of the lower end of the material guiding hopper 201 is adapted to the outer diameter of the trial preparation cylinder 203, and the trial preparation cylinder 203 is movably sleeved with the lower end opening of the material guiding hopper 201. The feed inlet 203a is placed inside the material guiding hopper 201. The upper end of the trial preparation cylinder 203 is connected to a sealing cover 206.

[0061] The trial preparation unit 200 is connected to a support plate 205. A piston cylinder 202 is installed on the main preparation unit 100. The piston cylinder telescopic rod 202a pushes and pulls the support plate 205 to realize the movement of the trial preparation unit 200. In this embodiment, the piston cylinder 202 uses a pneumatic cylinder. An upper cover is arranged at the upper part of the main preparation unit 100. The piston cylinder 202 is installed on the upper cover. An avoidance notch 101a for the support plate 205 to pass through is arranged on the upper cover. The trial preparation unit 200 rotates to make the support plate 205 correspond and fit with or be misaligned and separated from the avoidance notch 101a. The trial preparation unit 200 rotates to make the piston cylinder telescopic rod 202a be misaligned with the support plate 205. Two feed hoppers 107 are arranged on the upper cover, and the two feed hoppers 107 are arranged oppositely.

[0062] There are two piston cylinders 202. Limiting plates 207 are connected to the telescopic rods 202a of the two piston cylinders. One limiting plate 207 is placed below the support plate 205, and the other limiting plate 207 is placed above the support plate 205. The support plate 205 moves within the range between the two limiting plates 207. In this embodiment, there are two support plates 205, and both support plates 205 are in an arc structure. The two support plates 205 are circumferentially spaced. The support plate 205 and the trial fitting cylinder 203 are fixedly connected by a connecting rod 108a. One limiting plate 207 is placed above one support plate 205, and the other limiting plate 207 is placed below the other support plate 205. Two avoidance notches 101a are correspondingly arranged on the upper cover and the two support plates 205. The avoidance notches 101a are arranged with a 90-degree dislocation from the support plate 205. Insertion holes are correspondingly arranged on the upper cover and the trial fitting cylinder 203. The trial fitting cylinder 203 is inserted and connected with the insertion holes. A through groove is arranged between the avoidance notch 101a and the insertion hole, which facilitates the connecting rod 204 to pass through. The width of the limiting plate 207 is smaller than the gap between the two support plates 205, which facilitates the limiting plate 207 to pass through the gap between the two support plates 205 after the trial fitting cylinder 203 rotates 90 degrees.

[0063] The active stirring assembly includes a stirring shaft 108. The stirring shaft 108 is rotatably installed in the preparation tank 101, and a number of stirring rods 109 are installed on the stirring shaft 108. The stirring rods 109 are arranged in several circles at intervals up and down, and four are evenly arranged in each circle. The lower end of the stirring shaft 108 extends out of the preparation tank 101 and is connected to the output shaft of the driving motor 104.

[0064] The driven stirring assembly includes a rotating shaft 208. The rotating shaft 208 is rotatably installed at the bottom of the trial fitting cylinder 203. The driven stirring assembly is arranged below the feeding port 203a. Stirring blades 210 are installed on the upper part of the rotating shaft 208. A number of stirring blades 210 are arranged at circumferential intervals. A turntable 211 is arranged at the lower part of the rotating shaft 208. The turntable 211 is placed outside the trial fitting cylinder 203. Two slots 212 are arranged on the turntable 211. Two inserting rods 213 are arranged on the active stirring assembly. The inserting rods 213 are arranged on the mounting plate 108b. The size of the slot 212 is larger than the outer diameter of the inserting rod 213. The inserting rod 213 is inserted into the slot 212 so that the active stirring assembly drives the rotating shaft 208 to rotate. The stirring shaft 108 and the rotating shaft 208 are coaxially arranged, and the inserting rod 213 deviates from the axis of the stirring shaft 108. The lower end of the trial fitting cylinder 203 is detachably connected to the base 219, and the rotating shaft 208 is rotatably installed on the base 219.

[0065] A closing ring 214 is movably sleeved inside a trial matching cylinder 203. A slot hole 215 is correspondingly arranged on the closing ring 214 and corresponding to a feeding port 203a. A dial rod 216 protruding outwards is arranged on the closing ring 214. An abutting block 217 is arranged inside a material guiding hopper 201. A sliding groove 218 is correspondingly arranged on the trial matching cylinder 203 and corresponding to the dial rod 216. The dial rod 216 passes through the sliding groove 218. When the trial matching cylinder 203 rotates, the dial rod 216 abuts against the abutting block 217, so that the closing ring 214 and the trial matching cylinder 203 rotate relative to each other, and the closing ring 214 closes the feeding port 203a. When the trial matching cylinder 203 is installed into the main matching unit 100, the slot hole 215 is correspondingly communicated with the feeding port 203a. Before the trial matching unit 200 is detached from the main matching unit 100, first rotate the trial matching cylinder 203. When a support plate 205 is aligned with an avoidance notch 101a, the dial rod 216 abuts against the abutting block 217, so that the closing ring 214 and the trial matching cylinder 203 rotate relative to each other, and the closing ring 214 closes the feeding port 203a. At this time, a through groove is correspondingly arranged between the dial rod 216, the avoidance notch 101a and an insertion hole. The dial rod 216 can pass through the through groove, and finally the whole trial matching cylinder 203 is pulled out upwards. The closing ring 214 closes the feeding port 203a, avoiding the composite carbon source from flowing outwards from the feeding port 203a during the process of detaching the trial matching cylinder 203. When pouring the composite carbon source inside the trial matching cylinder 203 into the sampled sewage, it is avoided that the composite carbon source flows away from the feeding port 203a.

[0066] A method for preparing a composite carbon source based on gene sequencing, which is prepared by using a device for preparing a composite carbon source based on gene sequencing, includes the following steps: S1, collecting sewage samples, selecting sewage at different depth positions, and respectively filling them into different samplers; S2, performing gene sequencing on the bacteria in the sewage samples in the samplers, averaging the number of bacteria measured in the sewage samples taken at different depth positions, calculating the average value of the bacteria per unit volume, and obtaining the distribution information of the bacteria in the sewage; S3, selecting appropriate carbon source raw materials for preparation according to the obtained bacteria distribution information; the types of bacteria measured are Alcaligenes, Neisseriaceae, Rhodospirillaceae, Bacillaceae, Spirillaceae, and the carbon source raw materials with good treatment effects on these types of bacteria are glucose, sodium acetate, propylene glycol, acetic acid, methanol, and starch respectively. When preparing the carbon source raw materials, the quantity ratio of Alcaligenes, Neisseriaceae, Rhodospirillaceae, Bacillaceae, Spirillaceae is a:b:c:d:e, and the weight ratio of the corresponding glucose, sodium acetate, propylene glycol, acetic acid, methanol, and starch is also a:b:c:d:e. When preparing, first perform small-scale pre-preparation, load the carbon source raw materials into the test preparation unit 200, and the active stirring component runs and rotates to drive the driven stirring component to rotate; S4, putting the pre-prepared composite carbon source into the collected sewage samples for use; S5, observing whether the use effect reaches the expectation. If the use effect does not reach the expectation, repeat S3 and S4, adjust the carbon source raw materials and their ratios until the use effect reaches the expectation; S6, referring to the raw materials and ratios required for the composite carbon source with the expected use effect, perform large-scale preparation using the main preparation unit 100, and the active stirring component runs and rotates to stir and mix the raw materials to obtain a composite carbon source finished product that meets the expectation.

[0067] After scientifically sampling the sewage water body at multiple levels in this invention, then performing gene sequencing on the water body samples through the gene sequencing module to obtain the distribution information of the bacteria in the water body. According to the obtained bacteria distribution information, reasonably select carbon source raw materials for scientific preparation. Before preparation, perform small-scale pre-configuration through the test preparation unit 200, put the obtained finished product into the samples taken by the sampling module for use again until the use effect reaches the expectation, and then perform large-scale preparation through the main preparation unit 100 to obtain a composite carbon source finished product that meets the expectation.

[0068] Select carbon source raw materials to prepare a composite carbon source according to the bacteria distribution information in the sewage. The prepared composite carbon source has strong pertinence and good use effect. Moreover, in this application, first perform small-scale trial production, use the composite carbon source obtained from the trial production first, observe the use effect, and until the use effect reaches the expectation, then perform large-scale preparation of the composite carbon source to obtain the final product, with high production efficiency. It avoids the poor use effect of the composite carbon source obtained by directly performing large-scale preparation, resulting in waste and affecting production efficiency.

[0069] The above-described embodiments are only preferred solutions of the present invention and do not impose any formal restrictions on the present invention. There are other variations and modifications without exceeding the technical solutions described in the claims.

Claims

1. A composite carbon source preparation device based on gene sequencing, characterized in that: It includes a main mixing unit and a trial mixing unit, which can be detachably installed on the main mixing unit. An active stirring assembly is arranged in the main mixing unit, and a driven stirring assembly is arranged in the trial mixing unit. The active stirring assembly can drive the driven stirring assembly to rotate. A material guide hopper is arranged on the main mixing unit, and the trial mixing unit includes a trial mixing cylinder, which is connected with a sealing sleeve at the lower end of the material guide hopper, and a material inlet is arranged on the trial mixing cylinder. A closed ring is movably sleeved in the trial mixing cylinder, and slots are arranged on the closed ring corresponding to the material inlet. A lever extending outward is arranged on the closed ring, and an abutment block is arranged in the material guide hopper. The trial mixing cylinder rotates to make the lever press against the abutment block, thereby making the closed ring and the trial mixing cylinder rotate relative to each other, and the closed ring closes the material inlet.

2. A composite carbon source preparation device based on gene sequencing according to claim 1, characterized in that: The trial matching unit is connected to the support plate, and a piston cylinder is installed on the main matching unit. The telescopic rod of the piston cylinder pushes and pulls the support plate to realize the movement of the trial matching unit.

3. A composite carbon source preparation device based on gene sequencing according to claim 2, characterized in that the main An upper cover is arranged on the upper part of the fitting unit, and an avoidance gap is arranged on the upper cover for the support plate to pass through. The fitting unit is rotated to make the support plate and the avoidance gap correspondingly adapted or dislocated and separated, and the fitting unit is rotated to make the piston cylinder telescopic rod dislocated with the support plate.

4. A composite carbon source preparation device based on gene sequencing according to claim 3, characterized in that: Two piston cylinders are provided, and the telescopic rods of the two piston cylinders are connected with limit plates, one limit plate is placed below the support plate, and the other limit plate is placed above the support plate, and the support plate moves within the range between the two limit plates.

5. The composite carbon source preparation device based on gene sequencing according to claim 1 is characterized in that: The preparation unit comprises a preparation tank, and the active stirring assembly comprises a stirring shaft, which is rotatably installed in the preparation tank, and a plurality of stirring rods are installed on the stirring shaft.

6. A composite carbon source preparation device based on gene sequencing according to any one of claims 1 to 5, characterized in that: The driven stirring assembly includes a rotating shaft, on which a plurality of magnetic stirring blades are circumferentially spaced apart. A plurality of circumferentially spaced magnetic blocks are mounted on the upper end of the active stirring assembly. The magnetic blocks and the magnetic stirring blades attract each other, and the magnetic blocks rotate together with the active stirring assembly, thereby driving the magnetic stirring blades to rotate.

7. A composite carbon source preparation device based on gene sequencing according to any one of claims 1 to 5, characterized in that: The driven stirring assembly includes a rotating shaft, a stirring blade is installed on the upper part of the rotating shaft, a turntable is arranged at the lower part of the rotating shaft, a slot is arranged on the turntable, and an insertion rod is arranged on the active stirring assembly. The insertion rod is inserted into the slot so that the active stirring assembly drives the rotating shaft to rotate.

8. A method for preparing a composite carbon source based on gene sequencing, characterized in that: The preparation of the composite carbon source preparation device based on gene sequencing according to any one of claims 1 to 7 comprises the following steps: S1, collecting sewage samples, selecting sewage at different depths, and respectively placing them in different samplers; S2, performing gene sequencing on the bacterial species in the sewage samples in the samplers to obtain the distribution information of the bacterial species in the sewage; S3, selecting a suitable carbon source raw material for preparation according to the obtained bacterial species distribution information; during preparation, firstly carry out a small-scale pre-preparation, loading the carbon source raw material into the trial preparation unit, and the active stirring component is operated and rotated to drive the driven stirring component to rotate; S4, putting the pre-prepared composite carbon source into the collected sewage sample for use; S5, observing whether the use effect meets the expectations, and if the use effect does not meet the expectations, repeating S3 and S4, adjusting the carbon source raw material and the ratio, until the use effect meets the expectations; S6, referring to the raw materials and the ratio required for the composite carbon source to achieve the expected use effect, adopting the main preparation unit for large-scale preparation, and the active stirring component is operated and rotated to stir and mix the raw materials to obtain the composite carbon source finished product that meets the expectations.

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