A processing device for microfluidic visualization model and a method for using the same

Abstract

The present invention discloses a processing device for a microfluidic visualization model and a method for using the same, and relates to the field of microfluidic technology, including a bottom support platform, a microfluidic model frame and a fixture seat, wherein the top of the bottom support platform is provided with a microfluidic model frame, the top of the bracket is provided with a fixture seat, the unloading groove is provided on the inner wall of the chip groove, the L-shaped bracket is movably installed on the inner side of the unloading groove, and a translation hand lever is installed on the inner side of the L-shaped bracket. The present invention provides a slow-support docking assembly on the inner side of the fixture seat, and a soft-cushioned support plate supports the bottom of the microfluidic upper chip when the microfluidic upper chip is loaded, and the slow-shifting hand lever is operated and contracted to drive the soft-cushioned support plate to move downward, and the microfluidic upper chip continues to drop with the soft-cushioned support plate, and when the microfluidic upper chip moves into place, the translation hand lever contracts to store the soft-cushioned support plate inside the unloading groove, and the microfluidic lower chip falls above the chip gasket, and the alignment installation between the upper and lower microfluidic chips avoids interference with the fluid behavior caused by inadequate assembly.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, and in particular to a processing device for a microfluidic visualization model and a use method thereof. Background Art

[0002] Microfluidics technology is an emerging interdisciplinary subject involving chemistry, fluid physics, microelectronics, new materials, biology and biomedical engineering. It can precisely control the movement of droplets and realize operations such as droplet fusion and separation. The microfluidic visualization model is an experimental tool for studying and observing the behavior of microscale fluids. It can be used to study various fluid phenomena, such as droplet generation, fluid mixing, chemical reactions, etc. Special processing devices are used to effectively process and analyze the microfluidic visualization model.

[0003] The defects of the microfluidic processing device in the prior art are:

[0004] 1. Patent document US20140166133A1 discloses a microfluidic device and a microfluidic chip thereof. The microfluidic device cannot automatically assist in assembling the chip. Manual assembly can easily cause the upper and lower chips to collide and be damaged. In addition, improper assembly of the upper and lower chips will interfere with the fluid behavior.

[0005] 2. Patent document CN205361372U discloses a reversible microfluidic chip fixture. The connection between the fluid conduit and the chip in the chip fixture is not easy to disassemble, and the poor sealing between the two can easily lead to fluid leakage, which reduces the efficiency of microfluidic experiments and cannot guarantee the accuracy of experimental results.

[0006] 3. Patent document CN205182755U discloses a fixture for a sandwich structure microfluidic chip. The fixture has poor stability when injecting fluid into the chip. After the fluid injection is completed, the injection tube cannot be quickly closed. The residual fluid in the injection tube will continue to flow into the microfluidic chip, causing adverse effects on the experimental process.

[0007] 4. Patent document CN216499434U discloses a microfluidic device and an imaging device. The microfluidic device cannot adjust the light source at the acquisition position of the imaging device according to the external environment, which may easily lead to poor clarity of the image collected by the imaging device and fail to ensure the stability and accuracy of the image. Summary of the invention

[0008] The purpose of the present invention is to provide a processing device for a microfluidic visualization model and a method for using the same to solve the problems raised in the above background technology.

[0009] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a processing device for a microfluidic visualization model, comprising a bottom support platform, a microfluidic model frame and a fixture seat, wherein the microfluidic model frame is installed on the top of the bottom support platform, a support tube is installed on the inner side of the microfluidic model frame, a fixture seat is installed on the top of the support tube, and a chip slot is opened on the top of the fixture seat;

[0010] A buffer docking assembly is provided on the inner side of the clamp seat, and the buffer docking assembly includes a force unloading groove, a sinking groove, an L-shaped bracket and a padded support plate. The force unloading groove is opened on the inner wall of the chip groove, and the sinking groove is opened on the bottom wall of the force unloading groove. The L-shaped bracket is movably installed on the inner side of the force unloading groove, and a buffer hand rod is installed on the bottom wall of the sinking groove, and the telescopic end of the buffer hand rod is connected to the bottom of the L-shaped bracket. A translation hand rod is installed on the inner side of the L-shaped bracket, and the telescopic end of the translation hand rod is connected to the padded support plate.

[0011] Preferably, the top of the clamp seat is symmetrically provided with top slots, the inner side of the top slot is installed with a docking and fixing hand rod, the telescopic end of the docking and fixing hand rod is installed with a clamping upper cover, the top of the clamping upper cover is penetrated by a collecting port, the inner side of the collecting port is installed with a glass pressure plate, the top of the clamping upper cover is provided with a shading assembly, the shading assembly includes an arc frame, a fill light and a shading circular ring plate, the arc frame is installed on the top of the clamping upper cover, and the arc frame is located on the outer side of the collecting port, the fill light is installed on the inner wall of the arc frame, the shading circular ring plate is movably installed on the inner side of the arc frame, a height adjustment hand rod is installed on the inner side of the arc frame, and the telescopic end of the height adjustment hand rod is connected to the bottom of the shading circular ring plate.

[0012] Preferably, a guide rail is installed on the top of the bottom support platform, a driving stand is installed on the output end of the guide rail, a distance-adjusting cylinder is installed on the bottom of the driving stand, and an optical microscope is installed on the output end of the distance-adjusting cylinder.

[0013] Preferably, a hollow column is installed on the top of the clamp seat, a docking groove is installed on the bottom of the clamping cover, and the hollow column cooperates with the docking groove, a limiting groove is opened on the bottom wall of the chip groove, a flow supply port is opened through the outer side of the clamp seat, a microfluidic upper chip is installed on the inner side of the chip groove, a microfluidic lower chip is installed on the inner side of the limiting groove, a chip gasket is installed on the top of the microfluidic lower chip, a liquid inlet is opened on the outer side of the microfluidic upper chip, and a sealing groove is opened on the inner wall of the liquid inlet.

[0014] Preferably, a fixed outer frame is installed on the outer side of the microfluidic model frame, a loading cylinder is installed on the outer side of the fixed outer frame, a fluid sealing assembly is provided at the piston end of the loading cylinder, the fluid sealing assembly includes a synchronous cross plate, a docking tube and an inflatable rubber ring, the synchronous cross plate is connected to the piston end of the loading cylinder, the docking tube passes through the outer side of the synchronous cross plate, and the docking tube passes through the flow supply port and cooperates with the liquid inlet, an outer side of the docking tube is provided with an external groove, the inflatable rubber ring is installed on the inner side of the external groove, and the inflatable rubber ring cooperates with the sealing groove.

[0015] Preferably, a fluid tank is installed on the top of the bottom support platform, and a flow supply micropump is installed on the top of the bottom support platform. The input end of the flow supply micropump is connected to the fluid tank, and the output end of the flow supply micropump is connected to the butt joint through a pipeline. An air supply pump is installed on the top of the bottom support platform, and the output end of the air supply pump is connected to the air inlet end of the inflatable rubber ring through a pipeline. A regulating valve stem is installed on the outside of the butt joint, and a baffle valve plate is installed on the output end of the regulating valve stem.

[0016] Preferably, a flip roller is installed inside the rear end of the bottom support platform through a bearing, a sealed motor is installed on the outer side of the bottom support platform, and the output end of the sealed motor is connected to the input end of the flip roller, a sealing cover is installed on the outer side of the flip roller, a heater is installed on the inner wall of the sealing cover, a cooler is installed on the inner wall of the sealing cover, and a temperature sensor is installed on the inner wall of the sealing cover.

[0017] Preferably, a microfluidic acquisition processor is installed on the top of the bottom support platform, and the microfluidic acquisition processor consists of a feeding drive module and an image acquisition module. The feeding drive module is bidirectionally electrically connected to the buffer docking assembly, the docking fixing handle, the fluid sealing assembly, the flow supply micropump and the sealing motor. The image acquisition module is bidirectionally electrically connected to the shading assembly, the distance adjustment cylinder and the optical microscope. The image acquisition module is bidirectionally electrically connected to a clarity contrast unit and a data processing and analysis unit.

[0018] Preferably, the working steps of the processing device are as follows:

[0019] S1. First, assemble the microfluidic chip, place the microfluidic lower chip inside the limit groove, place the microfluidic upper chip inside the chip groove, and support the bottom of the microfluidic upper chip with a soft cushion support plate. The soft cushion support plate moves downward by contraction of the slow-moving hand lever. The soft cushion support plate continues to support the microfluidic upper chip during the descent process, and the microfluidic upper chip continues to descend with the soft cushion support plate.

[0020] S2. The docking fixing hand lever works to pull the clamping upper cover downward, the clamping upper cover fits with the upper surface of the fixture seat, the hollow column is inserted into the inner side of the docking groove, the glass pressure plate presses and limits the upper surface of the microfluidic chip, the loading cylinder works to push the synchronous cross plate, the synchronous cross plate drives the docking tube to move towards the direction close to the fixture seat, the docking tube passes through the flow supply port and is inserted into the inner side of the liquid inlet, the air supply pump works to inflate the inflatable rubber ring, and the inflatable rubber ring expands to fill the connection gap between the outer end of the docking tube and the sealing groove;

[0021] S3, the sealing motor drives the flip roller to rotate so that the sealing cover flips to seal the top of the bottom support platform. The experimental temperature is accurately adjusted by the heater and cooler according to the experimental requirements. The guide rail drives the stand to move to the top of the collection port. The distance adjustment cylinder drives the optical microscope to move downward so that it is located inside the collection port. The flow supply micropump operates to extract the fluid in the fluid tank. The fluid is injected into the microfluidic chip through the docking tube. The flow supply micropump controls the flow rate and flow velocity of the fluid.

[0022] S4, the microfluidic acquisition processor works by converting the collected images into digital signals through the data processing and analysis unit, extracting quantitative information such as flow rate and droplet size of the fluid behavior, and performing further data analysis to realize the processing of the microfluidic process.

[0023] Preferably, in step S1, the following steps are also included:

[0024] S11, when the upper microfluidic chip is about to contact the lower microfluidic chip, the translation hand lever is operated, and the translation hand lever is contracted to store the soft cushion support plate into the force unloading groove, so as to release the obstruction of the soft cushion support plate to the lower microfluidic chip, and the lower microfluidic chip falls to the top of the chip gasket;

[0025] In step S3, the following steps are also included:

[0026] S31. When the optical microscope detects that the clarity of the collected image is not good through the clarity contrast unit, the shading component operates, the hand lever is raised to push the shading ring plate upward, the fill light is turned on to fill light on the inside of the arc frame, and the optical microscope collects the fluid behavior in the microfluidic chip.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] 1. The present invention provides a slow-support docking assembly on the inner side of the fixture seat. When the microfluidic upper chip is loaded, the soft-padded support plate supports its bottom. The slow-moving hand lever contracts and drives the soft-padded support plate to move downward. The soft-padded support plate continues to support the microfluidic upper chip during its descent. The microfluidic upper chip continues to descend with the soft-padded support plate. When the microfluidic upper chip and the microfluidic lower chip are about to contact, the translation hand lever works and contracts to store the soft-padded support plate into the unloading groove, thereby removing the obstruction of the soft-padded support plate to the microfluidic lower chip. The microfluidic lower chip falls above the chip gasket, preventing the microfluidic upper chip and the microfluidic lower chip from colliding and being damaged due to manual placement. The alignment installation between the microfluidic upper and lower chips avoids interference with the fluid behavior caused by inadequate assembly.

[0029] 2. The present invention provides a fluid sealing assembly at the piston end of the loading cylinder. The docking and fixing hand lever works to pull the clamping cover downward, and the clamping cover fits with the upper surface of the fixture seat. The hollow column is inserted into the inner side of the docking groove. The glass pressure plate presses and limits the upper surface of the microfluidic chip. The upper and lower fixation of the microfluidic chip ensures its placement stability while facilitating the disassembly and replacement of the microfluidic chip. The loading cylinder works to push the synchronous cross plate, and the synchronous cross plate drives the docking tube to move toward the fixture seat. The docking tube passes through the flow supply port and is inserted into the inner side of the liquid inlet. The air supply pump works to inflate the inflatable rubber ring. The inflatable rubber ring expands to fill the connection gap between the outer end of the docking tube and the sealing groove. The good sealing performance prevents the leakage of the injected fluid, thereby greatly improving the experimental efficiency and accuracy of microfluidics.

[0030] 3. The present invention installs a regulating valve stem on the outer side of the butt joint, and the flow supply micropump is operated to extract the fluid in the fluid tank, and the fluid is injected into the microfluidic chip through the butt joint. The flow supply micropump accurately controls the flow rate and flow velocity of the fluid to ensure the stability of the fluid flow velocity. When the fluid injection is stopped, the flow supply micropump stops running and the regulating valve stem drives the baffle valve plate to rotate to achieve rapid blocking of the fluid, thereby preventing the residual fluid in the butt joint from continuing to flow into the microfluidic chip and causing adverse effects on the experimental process.

[0031] 4. The present invention provides a shading component on the top of the clamping upper cover. When the optical microscope detects that the clarity of the collected image is not good through the clarity comparison unit, the shading component is operated, the hand lever is adjusted to push the shading circular ring plate to move upward, the fill light is turned on to fill light on the inner side of the arc frame, and the optical microscope collects the fluid behavior in the microfluidic chip. The gathering of scattered light and the fill light can help improve the brightness and contrast of the image, improve the clarity of the image collected by the optical microscope, thereby improving the image quality and ensuring the stability and accuracy of the image to ensure that the image meets the analysis requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1It is a schematic diagram of the three-dimensional structure of the present invention;

[0033] Figure 2 It is a schematic diagram of the three-dimensional structure of the clamping upper cover of the present invention;

[0034] Figure 3 It is a schematic diagram of the internal structure of the liquid inlet of the present invention;

[0035] Figure 4 It is a schematic diagram of the three-dimensional structure of the microfluidic chip of the present invention;

[0036] Figure 5 It is a schematic diagram of the internal structure of the clamp seat of the present invention;

[0037] Figure 6 It is a schematic diagram of the three-dimensional structure of the butt-joint pipe of the present invention;

[0038] Figure 7 It is a schematic diagram of the internal structure of the butt-joint pipe side of the present invention;

[0039] Figure 8 It is a schematic diagram of the sealing cover connection structure of the present invention;

[0040] Fig. 9 A diagram of a microfluidic acquisition processor system of the present invention;

[0041] Fig.10 It is the work flow chart of the present invention.

[0042] In the figure: 1, bottom support platform; 2, microfluidic model frame; 3, fixture seat; 4, clamping cover; 5, unloading groove; 6, fixed outer frame; 7, sealing cover; 8, microfluidic acquisition processor; 9, guide rail; 10, driving stand; 11, adjustable cylinder; 12, optical microscope; 13, fluid tank; 14, flow supply micro pump; 15, air supply pump; 16, support tube; 17, top slot; 18, docking and fixing handle; 19, hollow column; 20, flow supply port; 21, chip slot; 22, limit slot; 23, microfluidic upper chip; 24, microfluidic lower chip; 25, chip gasket; 26, Liquid inlet; 27. Sealing groove; 28. Docking groove; 29. ​​Collection port; 30. Glass pressure plate; 31. Arc frame; 32. Fill light; 33. Height adjustment lever; 34. Shading ring plate; 35. Sinking trough; 36. Slow-shift lever; 37. L-shaped bracket; 38. Translation lever; 39. Padded support plate; 40. Feeding cylinder; 41. Synchronous cross plate; 42. Docking tube; 43. Adjusting valve stem; 44. Baffle valve plate; 45. External slot; 46. Inflatable rubber ring; 47. Flip roller; 48. Sealing motor; 49. Heater; 50. Cooler; 51. Temperature sensor. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0045] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection or a movable connection, or a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0046] See also Figure 1 , Figure 2 , Figure 4 and Figure 5 , an embodiment provided by the present invention: a processing device for a microfluidic visualization model and a method for using the same;

[0047] The top of the fixture seat 3 is provided with a chip slot 21, and the inner side of the fixture seat 3 is provided with a slow-support docking assembly, which includes a force-unloading slot 5, a sinking slot 35, an L-shaped bracket 37 and a cushioned support plate 39. The force-unloading slot 5 is provided on the inner wall of the chip slot 21, and the sinking slot 35 is provided on the bottom wall of the force-unloading slot 5. The L-shaped bracket 37 is movably installed on the inner side of the force-unloading slot 5, and a slow-shifting hand rod 36 is installed on the bottom wall of the sinking slot 35, and the telescopic end of the slow-shifting hand rod 36 is connected to the bottom of the L-shaped bracket 37, and a translation hand rod 38 is installed on the inner side of the L-shaped bracket 37, and the telescopic end of the translation hand rod 38 is connected to the cushioned support plate 39;

[0048] The sinking groove 35 provides an installation position for the slow-moving hand rod 36, and the L-shaped bracket 37 provides an installation position for the translation hand rod 38 while ensuring its stability during operation. The force unloading groove 5 provides a movable space for the L-shaped bracket 37. When the microfluidic upper chip 23 is loaded, the soft pad support plate 39 supports its bottom, and the slow-moving hand rod 36 works and contracts to drive the soft pad support plate 39 to move downward. During the descent of the soft pad support plate 39, the microfluidic upper chip 23 continues to support the microfluidic upper chip 23. The microfluidic upper chip 23 continues to descend with the soft pad support plate 39. When the microfluidic upper chip 23 and the microfluidic lower chip 24 are about to contact, the translation hand rod 38 works, and the translation hand rod 38 works and contracts to store the soft pad support plate 39 into the force unloading groove 5, thereby removing the obstruction of the soft pad support plate 39 to the microfluidic lower chip 24, and the microfluidic lower chip 24 falls to above the chip gasket 25, thereby preventing the microfluidic upper chip 23 and the microfluidic lower chip 24 from colliding and being damaged due to manual placement.

[0049] See also Figure 1 , Figure 2 and Figure 3 , a processing device for a microfluidic visualization model and a method for using the same;

[0050] The top of the clamp seat 3 is symmetrically provided with a top slot 17, the inner side of the top slot 17 is provided with a docking fixing handle 18, the telescopic end of the docking fixing handle 18 is provided with a clamping cover 4, the top of the clamping cover 4 is provided with a collection port 29, the inner side of the collection port 29 is provided with a glass pressing plate 30, the top of the clamping cover 4 is provided with a shading component, the shading component includes an arc frame 31, a fill light 32 and a shading circular ring plate 34, the arc frame 31 is installed on the top of the clamping cover 4, and the arc frame 31 is located at the top of the clamping cover 4. On the outside of the collection port 29, a fill light 32 is installed on the inner wall of the arc frame 31, a light shielding circular ring plate 34 is movably installed on the inner side of the arc frame 31, a height adjustment lever 33 is installed on the inner side of the arc frame 31, and the telescopic end of the height adjustment lever 33 is connected to the bottom of the light shielding circular ring plate 34, a guide rail 9 is installed on the top of the bottom support platform 1, a driving stand 10 is installed on the output end of the guide rail 9, a distance adjustment cylinder 11 is installed on the bottom of the driving stand 10, and an optical microscope 12 is installed on the output end of the distance adjustment cylinder 11;

[0051] The top slot 17 provides an installation position for the docking and fixing handle bar 18, which adjusts the vertical distance between the fixture seat 3 and the clamping upper cover 4, and collects the fluid behavior image through the collection port 29. When the clarity of the collected image is not good, the shading component operates, and the height adjustment handle 33 works to push the shading ring plate 34 to move it upward. The fill light 32 lights up to fill light on the inside of the arc frame 31, and the optical microscope 12 collects the fluid behavior in the microfluidic chip. The gathering of scattered light and the fill light can help improve the brightness and contrast of the image, improve the image quality and ensure the stability and accuracy of the image. The guide rail 9 drives the drive stand 10 to adjust the position in the horizontal direction, which is convenient for providing space for loading and unloading microfluidic chips, and the distance adjustment cylinder 11 adjusts the use height of the optical microscope 12.

[0052] See also Figure 1 , Figure 2 , Figure 3 and Figure 4 , a processing device for a microfluidic visualization model and a method for using the same;

[0053] The microfluidic model frame 2 is installed on the top of the bottom support platform 1, and a support tube 16 is installed on the inner side of the microfluidic model frame 2. A collection port 29 is provided through the top of the clamping upper cover 4, and a glass pressing plate 30 is installed on the inner side of the collection port 29. A hollow column 19 is installed on the top of the clamp seat 3, and a docking groove 28 is installed on the bottom of the clamping upper cover 4, and the hollow column 19 cooperates with the docking groove 28. A limiting groove 22 is provided on the bottom wall of the chip groove 21, and a flow supply port 20 is provided through the outer side of the clamp seat 3. A microfluidic upper chip 23 is installed on the inner side of the chip groove 21, a microfluidic lower chip 24 is installed on the inner side of the limiting groove 22, and a chip gasket 25 is installed on the top of the microfluidic lower chip 24;

[0054] Microfluidic experiments are carried out through the microfluidic model frame 2, the bracket 16 supports the clamp seat 3, the microfluidic lower chip 24 is placed on the inner side of the limit groove 22, and the microfluidic upper chip 23 is placed on the inner side of the chip groove 21, and the docking and fixing handle 18 works to pull the clamping cover 4 to move downward, and the clamping cover 4 is fitted with the upper surface of the clamp seat 3, and the hollow column 19 is inserted into the inner side of the docking groove 28 to realize the limit connection between the clamp seat 3 and the clamping cover 4, and the glass pressure plate 30 presses and limits the upper surface of the microfluidic upper chip 23, and the upper and lower fixation of the microfluidic chip ensures its placement stability while facilitating the disassembly and replacement of the microfluidic chip.

[0055] See also Figure 1 , Figure 3 , Figure 6 and Figure 7 , a processing device for a microfluidic visualization model and a method for using the same;

[0056] The microfluidic model frame 2 includes a fixed outer frame 6 installed on the outer side, a feeding cylinder 40 installed on the outer side of the fixed outer frame 6, a fluid sealing component is provided at the piston end of the feeding cylinder 40, and the fluid sealing component includes a synchronous cross plate 41, a butt joint pipe 42 and an inflatable rubber ring 46. The synchronous cross plate 41 is connected to the piston end of the feeding cylinder 40, the butt joint pipe 42 penetrates the outer side of the synchronous cross plate 41, and the butt joint pipe 42 penetrates the flow supply port 20 and cooperates with the liquid inlet 26, an outer groove 45 is provided on the outer side of the butt joint pipe 42, an inflatable rubber ring 46 is installed on the inner side of the outer groove 45, and the inflatable rubber ring 46 cooperates with the sealing groove 27, a liquid inlet 26 is provided on the outer side of the microfluidic upper chip 23, and a sealing groove 27 is provided on the inner wall of the liquid inlet 26;

[0057] A fluid tank 13 is installed on the top of the bottom support platform 1, and a flow supply micro pump 14 is installed on the top of the bottom support platform 1. The input end of the flow supply micro pump 14 is connected to the fluid tank 13, and the output end of the flow supply micro pump 14 is connected to the butt joint 42 through a pipeline. An air supply pump 15 is installed on the top of the bottom support platform 1, and the output end of the air supply pump 15 is connected to the air inlet end of the inflatable rubber ring 46 through a pipeline. A regulating valve stem 43 is installed on the outer side of the butt joint 42, and a baffle valve plate 44 is installed on the output end of the regulating valve stem 43;

[0058] The fixed outer frame 6 provides an installation position for the loading cylinder 40 while ensuring its stability during operation. The loading cylinder 40 works to push the synchronous cross plate 41, and the synchronous cross plate 41 drives the docking tube 42 to move in the direction close to the fixture seat 3. The docking tube 42 passes through the flow supply port 20 and is plugged into the inner side of the liquid inlet 26. After the docking tube 42 moves into place, the air supply pump 15 works to inflate the inflatable rubber ring 46. The inflatable rubber ring 46 expands to fill the connection gap between the outer end of the docking tube 42 and the sealing groove 27. Good sealing performance prevents leakage of the injected fluid. The flow supply micropump 14 runs to extract the fluid in the fluid tank 13, and the fluid is injected into the microfluidic chip 23 through the docking tube 42. The flow supply micropump 14 controls the flow rate and flow velocity of the fluid. When the fluid injection is stopped, the flow supply micropump 14 stops running and the regulating valve stem 43 works to drive the baffle valve plate 44 to rotate to achieve rapid blocking of the fluid, thereby preventing the residual fluid in the docking tube 42 from continuing to flow into the microfluidic chip.

[0059] See also Figure 1 , Figure 8 and Fig. 9 , a processing device for a microfluidic visualization model and a method for using the same;

[0060] A turning roller 47 is installed inside the rear end of the bottom support platform 1 through a bearing, a sealing motor 48 is installed on the outside of the bottom support platform 1, and the output end of the sealing motor 48 is connected to the input end of the turning roller 47, a sealing cover 7 is installed on the outside of the turning roller 47, a heater 49 is installed on the inner wall of the sealing cover 7, a cooler 50 is installed on the inner wall of the sealing cover 7, a temperature sensor 51 is installed on the inner wall of the sealing cover 7, and a microfluidic acquisition processor 8 is installed on the top of the bottom support platform 1;

[0061] The microfluidic acquisition processor 8 is composed of a feeding drive module and an image acquisition module. The optical microscope 12 detects the clarity of the acquired image through a clarity comparison unit. The image acquisition module is bidirectionally electrically connected to the clarity comparison unit and the data processing and analysis unit. The microfluidic acquisition processor 8 converts the acquired image into a digital signal through the data processing and analysis unit, extracts quantitative information such as the flow rate and droplet size of the fluid behavior, and performs further data analysis to realize the processing of the microfluidic process. The microfluidic acquisition processor 8 integrates the components in each component and drives them;

[0062] The sealing motor 48 drives the turning roller 47 to rotate, so that the sealing cover 7 turns over to seal the top of the bottom support platform 1. The experimental temperature is accurately adjusted by the heater 49 and the cooler 50 according to the experimental requirements. The guide rail 9 drives the stand 10 to move to the top of the collection port 29. The distance adjustment cylinder 11 drives the optical microscope 12 to move downward so that it is located inside the collection port 29.

[0063] The working steps of the processing device are as follows:

[0064] S1. First, assemble the microfluidic chip. Place the microfluidic lower chip 24 inside the limit groove 22, and place the microfluidic upper chip 23 inside the chip groove 21. The soft cushion support plate 39 supports the bottom of the microfluidic upper chip 23. The slow-moving hand lever 36 contracts and drives the soft cushion support plate 39 to move downward. The soft cushion support plate 39 continues to support the microfluidic upper chip 23 during the descent process. The microfluidic upper chip 23 continues to descend with the soft cushion support plate 39.

[0065] S2, the docking fixing handle 18 works to pull the clamping upper cover 4 to move downward, the clamping upper cover 4 is fitted with the upper surface of the fixture seat 3, the hollow column 19 is inserted into the inner side of the docking groove 28, the glass pressing plate 30 presses and limits the upper surface of the microfluidic chip 23, the feeding cylinder 40 works to push the synchronous cross plate 41, the synchronous cross plate 41 drives the docking tube 42 to move towards the direction close to the fixture seat 3, the docking tube 42 passes through the flow supply port 20 and is inserted into the inner side of the liquid inlet 26, the air supply pump 15 works to inflate the inflatable rubber ring 46, and the inflatable rubber ring 46 expands to fill the connection gap between the outer end of the docking tube 42 and the sealing groove 27;

[0066] S3, the sealing motor 48 is operated to drive the flip roller 47 to rotate so that the sealing cover 7 flips to seal the top of the bottom support platform 1, and the experimental temperature is accurately adjusted by the heater 49 and the cooler 50 according to the experimental requirements. The guide rail 9 works to drive the stand 10 to move to the top of the collection port 29, and the distance adjustment cylinder 11 works to drive the optical microscope 12 to move downward so that it is located inside the collection port 29. The flow supply micro pump 14 is operated to extract the fluid in the fluid tank 13, and the fluid is injected into the microfluidic chip 23 through the docking pipe 42. The flow supply micro pump 14 controls the flow rate and flow rate of the fluid;

[0067] S4, the microfluidic acquisition processor 8 converts the collected images into digital signals through the data processing and analysis unit, extracts quantitative information such as flow rate and droplet size of the fluid behavior, and performs further data analysis to realize the processing of the microfluidic process.

[0068] In step S1, the following steps are also included:

[0069] S11, when the upper microfluidic chip 23 and the lower microfluidic chip 24 are about to contact, the translation lever 38 works, and the translation lever 38 contracts to store the soft cushion support plate 39 into the unloading groove 5, so as to release the obstruction of the soft cushion support plate 39 to the lower microfluidic chip 24, and the lower microfluidic chip 24 falls to the top of the chip gasket 25;

[0070] In step S3, the following steps are also included:

[0071] S31, when the optical microscope 12 detects that the clarity of the collected image is not good through the clarity comparison unit, the shading component operates, the hand lever 33 is adjusted to push the shading ring plate 34 to move it upward, the fill light 32 is turned on to fill light on the inside of the arc frame 31, and the optical microscope 12 collects the fluid behavior in the microfluidic chip.

[0072] Working principle: When using the device, the microfluidic chip is first assembled, the microfluidic lower chip 24 is placed on the inner side of the limit groove 22, and the microfluidic upper chip 23 is placed on the inner side of the chip groove 21. The soft pad support plate 39 supports the bottom of the microfluidic upper chip 23, and the slow-moving hand lever 36 works and contracts to drive the soft pad support plate 39 to move downward. The soft pad support plate 39 continues to support the microfluidic upper chip 23 during the descent process. The microfluidic upper chip 23 continues to descend with the soft pad support plate 39. When the microfluidic upper chip 23 is about to contact the microfluidic lower chip 24, the translation hand lever 38 works and contracts to store the soft pad support plate 39 into the unloading groove 5, thereby releasing the obstruction of the soft pad support plate 39 to the microfluidic lower chip 24, and the microfluidic lower chip 24 falls to the top of the chip gasket 25;

[0073] The docking fixing handle 18 works to pull the clamping upper cover 4 to move downward, and the clamping upper cover 4 fits with the upper surface of the fixture seat 3. The hollow column 19 is inserted into the inner side of the docking groove 28. The glass pressing plate 30 presses and limits the upper surface of the microfluidic chip 23. The loading cylinder 40 works to push the synchronous cross plate 41, and the synchronous cross plate 41 drives the docking tube 42 to move toward the direction close to the fixture seat 3. The docking tube 42 passes through the flow supply port 20 and is inserted into the inner side of the liquid inlet 26. The air supply pump 15 works to inflate the inflatable rubber ring 46. The inflatable rubber ring 46 expands to fill the connection gap between the outer end of the docking tube 42 and the sealing groove 27.

[0074] The sealing motor 48 drives the flip roller 47 to rotate so that the sealing cover 7 flips to seal the top of the bottom support platform 1. The experimental temperature is accurately adjusted by the heater 49 and the cooler 50 according to the experimental requirements. The guide rail 9 drives the stand 10 to move to the top of the collection port 29. The distance adjustment cylinder 11 drives the optical microscope 12 to move downward so that it is located inside the collection port 29. The flow supply micro pump 14 operates to extract the fluid in the fluid tank 13. The fluid is injected into the microfluidic chip 23 through the docking pipe 42. The flow supply micro pump 14 controls the flow rate and flow velocity of the fluid. When the optical microscope 12 detects that the clarity of the collected image is not good through the clarity comparison unit, the shading component operates, and the height adjustment lever 33 works to push the shading circular ring plate 34 to move it upward. The fill light 32 lights up to fill light on the inside of the arc frame 31, and the optical microscope 12 collects the fluid behavior in the microfluidic chip;

[0075] The microfluidic acquisition processor 8 converts the collected images into digital signals through the data processing and analysis unit, extracts quantitative information such as flow rate and droplet size of the fluid behavior, and performs further data analysis to realize the processing of the microfluidic process.

[0076] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A processing device for a microfluidic visualization model, comprising a bottom support platform (1), a microfluidic model frame (2) and a fixture seat (3), characterized in that: A microfluidic model frame (2) is installed on the top of the bottom support platform (1), a support tube (16) is installed on the inner side of the microfluidic model frame (2), a fixture seat (3) is installed on the top of the support tube (16), and a chip slot (21) is opened on the top of the fixture seat (3); A slow-support docking assembly is arranged on the inner side of the clamp seat (3), and the slow-support docking assembly comprises a force unloading groove (5), a sinking groove (35), an L-shaped bracket (37) and a cushion support plate (39), wherein the force unloading groove (5) is arranged on the inner wall of the chip groove (21), the sinking groove (35) is arranged on the bottom wall of the force unloading groove (5), the L-shaped bracket (37) is movably mounted on the inner side of the force unloading groove (5), a slow-shifting hand rod (36) is mounted on the bottom wall of the sinking groove (35), and the telescopic end of the slow-shifting hand rod (36) is connected to the bottom of the L-shaped bracket (37), and a translation hand rod (38) is mounted on the inner side of the L-shaped bracket (37), and the telescopic end of the translation hand rod (38) is connected to the cushion support plate (39); The top of the clamp seat (3) is provided with a hollow column (19), the bottom of the clamping upper cover (4) is provided with a docking groove (28), and the hollow column (19) cooperates with the docking groove (28), a limiting groove (22) is provided on the bottom wall of the chip groove (21), a flow supply port (20) is provided through the outer side of the clamp seat (3), a microfluidic upper chip (23) is provided on the inner side of the chip groove (21), a microfluidic lower chip (24) is provided on the inner side of the limiting groove (22), a chip gasket (25) is provided on the top of the microfluidic lower chip (24), a liquid inlet (26) is provided on the outer side of the microfluidic upper chip (23), and a sealing groove (27) is provided on the inner wall of the liquid inlet (26); A fixed outer frame (6) is installed on the outer side of the microfluidic model frame (2), and a feeding cylinder (40) is installed on the outer side of the fixed outer frame (6). A fluid sealing component is provided at the piston end of the feeding cylinder (40), and the fluid sealing component includes a synchronous cross plate (41), a butt joint pipe (42) and an inflatable rubber ring (46). The synchronous cross plate (41) is connected to the piston end of the feeding cylinder (40), and the butt joint pipe (42) passes through the outer side of the synchronous cross plate (41), and the butt joint pipe (42) passes through the flow supply port (20) and cooperates with the liquid inlet (26). An outer groove (45) is provided on the outer side of the butt joint pipe (42), and the inflatable rubber ring (46) is installed on the inner side of the outer groove (45), and the inflatable rubber ring (46) cooperates with the sealing groove (27).

2. A processing device for a microfluidic visualization model according to claim 1, characterized in that: The top of the clamp seat (3) is symmetrically provided with a top slot (17), the inner side of the top slot (17) is provided with a docking fixing handle (18), the telescopic end of the docking fixing handle (18) is provided with a clamping upper cover (4), the top of the clamping upper cover (4) is provided with a collection port (29), the inner side of the collection port (29) is provided with a glass pressing plate (30), and the top of the clamping upper cover (4) is provided with a shading component, the shading component comprises an arc frame (31), a fill light (32), and a plurality of glass pressing plates (30). 2) and a light-shielding circular plate (34), the arc frame (31) being mounted on the top of the clamping upper cover (4), and the arc frame (31) being located on the outside of the collection port (29), the fill light (32) being mounted on the inner wall of the arc frame (31), the light-shielding circular plate (34) being movably mounted on the inner side of the arc frame (31), a height-adjusting handle bar (33) being mounted on the inner side of the arc frame (31), and the telescopic end of the height-adjusting handle bar (33) being connected to the bottom of the light-shielding circular plate (34).

3. A processing device for a microfluidic visualization model according to claim 2, characterized in that: A guide rail (9) is installed on the top of the bottom support platform (1), a driving stand (10) is installed on the output end of the guide rail (9), a distance-adjusting cylinder (11) is installed on the bottom of the driving stand (10), and an optical microscope (12) is installed on the output end of the distance-adjusting cylinder (11).

4. The processing device for a microfluidic visualization model according to claim 1, characterized in that: A fluid tank (13) is installed on the top of the bottom support platform (1), a flow supply micro pump (14) is installed on the top of the bottom support platform (1), the input end of the flow supply micro pump (14) is connected to the fluid tank (13), and the output end of the flow supply micro pump (14) is connected to the butt joint pipe (42) through a pipeline, an air supply pump (15) is installed on the top of the bottom support platform (1), and the output end of the air supply pump (15) is connected to the air inlet end of the inflatable rubber ring (46) through a pipeline, an adjusting valve stem (43) is installed on the outside of the butt joint pipe (42), and a baffle valve plate (44) is installed on the output end of the adjusting valve stem (43).

5. The processing device for a microfluidic visualization model according to claim 1, characterized in that: A turning roller (47) is installed inside the rear end of the bottom support platform (1) via a bearing, a sealing motor (48) is installed on the outside of the bottom support platform (1), and the output end of the sealing motor (48) is connected to the input end of the turning roller (47), a sealing cover (7) is installed on the outside of the turning roller (47), a heater (49) is installed on the inner wall of the sealing cover (7), a cooler (50) is installed on the inner wall of the sealing cover (7), and a temperature sensor (51) is installed on the inner wall of the sealing cover (7).

6. The processing device for a microfluidic visualization model according to claim 1, characterized in that: A microfluidic acquisition processor (8) is installed on the top of the bottom support platform (1). The microfluidic acquisition processor (8) consists of a feeding drive module and an image acquisition module. The feeding drive module is bidirectionally electrically connected to a support docking assembly, a docking fixing handle (18), a fluid sealing assembly, a flow supply micro pump (14) and a sealing motor (48). The image acquisition module is bidirectionally electrically connected to a shading assembly, a distance adjustment cylinder (11) and an optical microscope (12). The image acquisition module is bidirectionally electrically connected to a clarity comparison unit and a data processing and analysis unit.

7. A method for using a microfluidic visualization model processing device according to any one of claims 1 to 6, characterized in that: The working steps of the processing device are as follows: S1. First, assemble the microfluidic chip, place the microfluidic lower chip (24) inside the limiting groove (22), place the microfluidic upper chip (23) inside the chip groove (21), and support the bottom of the microfluidic upper chip (23) with a soft cushion support plate (39). The slow-moving hand lever (36) contracts to drive the soft cushion support plate (39) to move downward. The soft cushion support plate (39) continues to support the microfluidic upper chip (23) during the descent process, and the microfluidic upper chip (23) continues to descend along with the soft cushion support plate (39); S2, the docking fixing handle (18) works to pull the clamping cover (4) downward, the clamping cover (4) fits with the upper surface of the fixture seat (3), the hollow column (19) is inserted into the inner side of the docking groove (28), the glass pressure plate (30) presses and limits the upper surface of the microfluidic chip (23), the loading cylinder (40) works to push the synchronous cross plate (41), the synchronous cross plate (41) drives the docking tube (42) to move in the direction close to the fixture seat (3), the docking tube (42) passes through the flow supply port (20) and is inserted into the inner side of the liquid inlet (26), the air supply pump (15) works to inflate the inflatable rubber ring (46), and the inflatable rubber ring (46) expands to fill the connection gap between the outer end of the docking tube (42) and the sealing groove (27); S3, the sealing motor (48) operates to drive the flip roller (47) to rotate so that the sealing cover (7) flips to seal the top of the bottom support platform (1), and the experimental temperature is accurately adjusted by the heater (49) and the cooler (50) according to the experimental requirements. The guide rail (9) works to drive the stand (10) to move to the top of the collection port (29), and the distance adjustment cylinder (11) works to drive the optical microscope (12) to move downward so that it is located inside the collection port (29). The flow supply micro pump (14) operates to extract the fluid in the fluid tank (13), and the fluid is injected into the microfluidic chip (23) through the docking pipe (42). The flow supply micro pump (14) controls the flow rate and flow velocity of the fluid; S4, the microfluidic acquisition processor (8) converts the collected images into digital signals through the data processing and analysis unit, extracts quantitative information such as flow rate and droplet size of the fluid behavior, and performs further data analysis to realize the processing of the microfluidic process.

8. The method for using the microfluidic visualization model processing device according to claim 7, characterized in that: In the step S1, the following steps are also included: S11, when the microfluidic upper chip (23) and the microfluidic lower chip (24) are about to contact, the translation hand lever (38) works, and the translation hand lever (38) works to contract and store the soft cushion support plate (39) into the unloading groove (5), thereby removing the obstruction of the soft cushion support plate (39) to the microfluidic lower chip (24), and the microfluidic lower chip (24) falls to the top of the chip gasket (25); In step S3, the following steps are also included: S31, when the optical microscope (12) detects that the clarity of the collected image is poor through the clarity comparison unit, the shading component operates, the hand lever (33) is adjusted to push the shading circular ring plate (34) to move it upward, the fill light (32) is turned on to fill light on the inner side of the arc frame (31), and the optical microscope (12) collects the fluid behavior in the microfluidic chip.

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