An indoor closed sargassum culture system and culture method

Abstract

This invention relates to an indoor closed-loop Sargassum culture system and method. The sterilization chamber is an opaque chamber containing a sterilization device and a first pipeline. The first pipeline has a return branch, a first output branch, and a control valve at its upper end. The regulating chamber has a second pipeline and a carbon dioxide regulating system, with the second pipeline connected to the culture chamber. The pH value of the water is controlled by the carbon dioxide regulating system, which regulates the carbon dioxide input. The culture chamber contains a substrate and an oxygen regulating system. A lighting system is located above the culture chamber. The substrate has a height-adjustable telescopic rod, and the Sargassum algae are placed on the telescopic rod. The water temperatures in both the regulating and culture chambers are regulated by a temperature regulating system. This invention provides large-volume, closed-loop culture, simulating the wild growth mode and environment of Sargassum algae, enabling rapid growth, development, and morphogenesis of the algae.

Description

Technical Field

[0001] This invention relates to the field of large algae cultivation technology, specifically to an indoor closed-loop Sargassum cultivation system and cultivation method. Background Technology

[0002] Indoor cultivation is an important approach for studying macroalgae. However, current indoor cultivation methods for macroalgae mainly involve small-body cultivation, suitable for filamentous algae, some small foliate algae, or microscopic generations of sporophytes or gametophytes (such as the sporophytes of Porphyra and the gametophytes of Kelp). Currently, only a few scholars have conducted research on the cultivation of macroalgae in open, large-body water. For example, Chinese invention patent CN106804418B discloses an indoor cultivation device for macroalgae, which includes a large water tank, a constant-temperature water tank, and several small water tanks. Through this structural combination, the device can provide diverse cultivation conditions. However, this device does not consider issues such as water sterilization and disinfection, or pH adjustment. Another example is Chinese invention patent CN103477969B, which discloses a closed cultivation device for macroalgae that facilitates culture medium replacement. This device includes culture bottles, a gas distributor, and a heat exchanger. The culture medium can be replaced without disassembly, but this device uses culture bottles to cultivate algae, resulting in a small number of algal strains.

[0003] Sargassum seaweed is a large brown algae group that mainly inhabits the low and subtidal zones of the ocean. It is a high-quality raw material for extracting substances such as alginate and mannitol, and is an important component of seaweed farms, possessing significant economic and ecological value. Structurally, Sargassum seaweed can be divided into three parts: the anchorage, the trunk, and the branches, making it a typical "branch-leaf" seaweed. However, due to its large size and lack of gametophytes (micro-generations), most Sargassum seaweeds are difficult to cultivate indoors for extended periods. Currently, indoor cultivation methods often involve cutting off branches and leaves for short-term cultivation in small water bodies under illumination. However, this often results in slow growth or even developmental abnormalities. Cultivation in open, large water bodies easily leads to bacterial and other algal growth, potentially causing the cultivation system to collapse. Summary of the Invention

[0004] The purpose of this invention is to provide an indoor closed Sargassum culture system and method, which is a large-volume, sealed culture that can simulate the outdoor growth mode and environment of Sargassum strains, and can achieve rapid growth, development and morphogenesis of Sargassum strains.

[0005] The objective of this invention is achieved through the following technical solution:

[0006] An indoor closed-loop Sargassum cultivation system includes an incubator unit, which comprises a sterilization chamber, a regulating chamber, a cultivation chamber, and a temperature regulation system. The sterilization chamber is an opaque chamber with a water inlet at its upper end. The sterilization chamber contains a sterilization device and a first pipeline. The first pipeline has a return branch pipe, a first output branch pipe, and a control valve at its upper end. The return branch pipe is located at the upper end of the sterilization chamber, and the first output branch pipe is located at the upper end of the regulating chamber. The return branch pipe and the first output branch pipe are connected through the... The control valve controls the switching. The regulating chamber is equipped with a second pipeline and a carbon dioxide regulating system. The second pipeline is connected to the culture chamber. The pH value of the water is regulated by controlling the carbon dioxide input through the carbon dioxide regulating system. The culture chamber is equipped with a fixation substrate and an oxygen regulating system. A lighting system is provided above the culture chamber. A drain outlet is provided on the lower side of one end of the culture chamber. The fixation substrate is equipped with a height-adjustable telescopic rod, and the Sargassum algae strain is placed on the telescopic rod. The water temperature of the regulating chamber and the water temperature of the culture chamber are both regulated by a temperature regulating system.

[0007] The sterilization device is an ultraviolet sterilization lamp, the first pipeline is equipped with a first submersible pump, and the sterilization chamber is equipped with a water sterilization timer.

[0008] The carbon dioxide regulation system includes a first gas stone, a pH detection probe, a carbon dioxide tank, a controller, and a solenoid valve. The first gas stone is located on the bottom surface inside the regulation chamber, the pH detection probe is located on the top cover of the regulation chamber, and the carbon dioxide tank, controller, and solenoid valve are all located outside the regulation chamber. The first gas stone is connected to the carbon dioxide tank through a carbon dioxide input pipeline, and the solenoid valve is installed on the carbon dioxide input pipeline. The solenoid valve is controlled to open and close by the controller.

[0009] The regulating chamber is equipped with a first temperature regulating system, and the culture chamber is equipped with a second temperature regulating system. The first and second temperature regulating systems have the same structure, both including a heat exchange jacket and a heat exchanger. The heat exchange jacket is equipped with a heat exchange pipeline, and the heat exchange pipeline is connected to the heat exchanger. The lower end of the second pipeline is equipped with a second submersible pump, and the upper end is equipped with a second output branch pipe, which is located at the upper end of the culture chamber.

[0010] The temperature control system includes a heat exchanger and a circulation pipeline, wherein one end of the circulation pipeline is located in the control chamber and the other end is located in the culture chamber. The circulation pipeline is equipped with a circulation pump and a heat exchanger, and a second submersible pump is located at the lower end of the second pipeline.

[0011] The lighting system includes a light lamp and a light controller, and the light intensity of the light lamp is controlled and adjusted by the light controller.

[0012] The oxygen regulation system includes a second gas stone and an air pump. The second gas stone is located on the bottom surface of the culture chamber and is connected to the air pump through an oxygen input pipeline. An oxygen concentration sensor is installed in the culture chamber.

[0013] The fixation substrate in the culture chamber includes a fixation substrate base, and the fixation substrate base is provided with a telescopic rod and a support rod. The lower end of the Sargassum algae strain is fixed to the upper end of the telescopic rod by a fixing wire. The detection optical fiber is fixed to the support rod, and the detection probe of the detection optical fiber is aligned with the Sargassum algae strain. The detection optical fiber is externally connected to a chlorophyll fluorometer.

[0014] The telescopic rod includes multiple threaded sleeves connected sequentially from top to bottom; a sliding sleeve is fitted on the support rod, and a locking screw is provided on the sliding sleeve; multiple screw holes are provided on the support rod; the detection optical fiber is fixed on the sliding sleeve; and after the height of the sliding sleeve is determined, the locking screw is threaded into the corresponding screw hole.

[0015] An indoor closed-loop Sargassum culture system, comprising the following steps:

[0016] Step 1: Algae treatment. After collecting the main stem of Sargassum with initial branches, soak it in seawater potassium iodide solution for a set time to kill small and medium-sized animals, insect eggs, etc. attached to the algae. Then clean the surface of the algae and remove the attached miscellaneous algae and dirt. Place the cleaned algae in a glass container and ventilate for temporary cultivation. After a set number of days of temporary cultivation, clean the surface of the algae again.

[0017] Step 2: Treatment of the incubator unit before algal culture: Disinfect the incubator unit with disinfectant.

[0018] Step 3: Fixing the algal strains. The Sargassum main stem is divided into a set number of equal parts, each part including the main stem and primary branches. Then, each part of the algal strain is fixed to the upper end of the telescopic rod on the corresponding anchoring substrate in the culture chamber.

[0019] Step 4: Determine the range of parameters for each unit in the incubator and monitor and control them in real time during algae cultivation. The parameters of the incubator unit include water quality, pH value, water temperature, oxygen concentration, light intensity, and light cycle. Water quality is controlled by the sterilization chamber, pH value is controlled by the carbon dioxide regulation system in the regulation chamber, water temperature is controlled by the temperature regulation system, oxygen concentration is controlled by the oxygen regulation system in the cultivation chamber, and light intensity and light cycle are controlled by the light system above the cultivation chamber. During algae cultivation, the water is changed regularly and water quality parameters are recorded regularly.

[0020] The advantages and positive effects of this invention are as follows:

[0021] 1. This invention is a combination design of multiple sealed chambers, each with a different function. The sterilization chamber sterilizes the water, and after ultraviolet sterilization, the water does not need to come into contact with air, greatly reducing the risk of contamination of the cultivation system. At the same time, the sterilization chamber is an opaque chamber, which also avoids damage to the algae from ultraviolet rays. The regulating chamber achieves stable acidification and water temperature control of the water. Since the regulating chamber is set up separately, it avoids the impact of drastic fluctuations in the pH value of the water on the growth of the algae. The cultivation chamber can adjust parameters such as light, water temperature, and water oxygen concentration in real time according to the growth of the algae, thereby ensuring that the algae have a stable and controllable growth environment.

[0022] 2. The various compartments of this invention can be combined and disassembled as needed. When disassembling and assembling each compartment, it is not necessary to separately disassemble and install pipelines. During assembly, it is only necessary to ensure that the first output branch pipe is located inside the upper end of the regulating compartment and the second output branch pipe is located inside the upper end of the culture compartment to realize the water transfer between the compartments. Furthermore, the first temperature regulating system, carbon dioxide regulating system and other devices can be integrated into the first frame on the top cover of the regulating compartment, and the second temperature regulating system, lighting system and other devices can be integrated into the second frame on the top cover of the culture compartment. In this way, the two compartments can be transferred independently as a whole and can be operated independently, which improves the flexibility of use of this invention.

[0023] 3. The sterilization chamber of the present invention can switch between self-circulation state and output state through the control valve at the upper end of the first pipeline. In the self-circulation state, the water in the sterilization chamber flows through self-circulation to ensure thorough sterilization of the water.

[0024] 4. The immobilization substrate of the present invention can not only be used for the culture of algal larvae and adults and in situ activity monitoring, but the immobilization substrate base can also collect spores. The regular square design on the immobilization substrate base facilitates spore counting and testing, and the height of the telescopic rod and the detection optical fiber can be flexibly adjusted according to the growth of the algal strain.

[0025] 5. This invention is a large-volume, sealed culture that can simulate the wild growth mode and environment of Sargassum fusiforme, enabling rapid growth, development, and morphogenesis of Sargassum fusiforme. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the structure of one embodiment of the system of the present invention.

[0027] Figure 2 This is a schematic diagram of another embodiment of the system of the present invention.

[0028] Figure 3 for Figure 1 Schematic diagram of the structure of the intermediate fixation group.

[0029] Figure 4This diagram illustrates a comparison of the growth effects of algal strains cultured using existing technology devices with those cultured using the system of this invention.

[0030] Figure 5 This diagram illustrates a comparison of the growth rates of algal strains cultured using existing technology devices and those cultured using the system of this invention.

[0031] Figure 6 This is a schematic diagram comparing the photosynthetic activity of algal strains cultured using existing technology devices with that of algal strains cultured using the system of this invention.

[0032] Wherein, 1 is the anchoring substrate; 101 is the anchoring substrate base; 102 is the support rod; 103 is the telescopic rod; 1031 is the screw sleeve; 104 is the detection optical fiber; 105 is the sliding sleeve; 1051 is the locking screw; 106 is the fixing wire; 107 is the Sargassum algae strain; 108 is the grid line; 109 is the numbering mark; 2 is the incubator unit; 201 is the sterilization chamber; 2011 is the first pipeline; 2012 is the reflux branch pipe; 2013 is the first output branch pipe; 202 is the regulating chamber; 2021 is the second pipeline; 2022 is the carbon dioxide regulating system; 2023 is the first temperature regulating system; 2024 is the second output branch pipe; 203 is the culture... The compartment is as follows: 2031 is the oxygen regulation system; 2032 is the second temperature regulation system; 2033 is the lighting system; 204 is the sterilization device; 205 is the first submersible pump; 206 is the control valve; 207 is the water inlet; 208 is the heat exchange jacket; 2081 is the heat exchanger; 2082 is the heat exchange pipeline; 209 is the controller; 210 is the pH detection probe; 211 is the first air stone; 212 is the second submersible pump; 213 is the second air stone; 214 is the air pump; 215 is the lighting lamp; 216 is the light controller; 217 is the drain outlet; 218 is the wave generator pump; 219 is the carbon dioxide tank; 220 is the solenoid valve; 221 is the circulation pipeline. Detailed Implementation

[0033] The invention will now be described in further detail with reference to the accompanying drawings.

[0034] like Figures 1-3As shown, the present invention includes an incubator unit 2, which includes a sterilization chamber 201, a regulating chamber 202, an incubation chamber 203, and a temperature regulating system. The sterilization chamber 201 is an opaque chamber. An inlet 207 is provided at the upper end of the sterilization chamber 201. A sterilization device 204 and a first pipeline 2011 are provided inside the sterilization chamber 201. A return branch pipe 2012, a first output branch pipe 2013, and a control valve 206 are provided at the upper end of the first pipeline 2011. The return branch pipe 2012 is located at the upper end of the sterilization chamber 201, and the first output branch pipe 2013 is... At the upper end of the regulating chamber 202, and with the return branch pipe 2012 and the first output branch pipe 2013 controlled by the control valve 206, the regulating chamber 202 is equipped with a second pipe 2021 and a carbon dioxide regulating system 2022, wherein the second pipe 2021 is connected to the culture chamber 203, and the pH value of the water is regulated by controlling the carbon dioxide input through the carbon dioxide regulating system. The culture chamber 203 is equipped with a fixation substrate 1 and an oxygen regulating system 2031. A lighting system 2033 is provided above the culture chamber 203, and a drain outlet 217 is provided on the lower side of one end of the culture chamber 203. Figure 2 As shown, the fixation base 1 is provided with a height-adjustable telescopic rod 103, and the Sargassum fusiforme strain 107 is provided on the telescopic rod 103. The water temperature of the regulating chamber 202 and the water temperature of the culture chamber 203 are both regulated by a temperature regulation system.

[0035] like Figure 1As shown, the sterilization device 204 is a commercially available ultraviolet (UV) sterilization lamp. Additionally, a first submersible pump 205 is installed on the first pipeline 2011, through which water in the sterilization chamber 201 is pumped into the regulating chamber 202. In this embodiment, the walls and top cover of the sterilization chamber 201 are made of black, opaque acrylic material to prevent the UV sterilization lamp inside the chamber from affecting the algae. When the present invention is in operation, the first output branch pipe 2013 at the upper end of the first pipeline 2011 is closed first, while the return branch pipe 2012 is opened. The above operation is controlled by the control valve 206. At this time, the water in the sterilization chamber 201 flows through the first pipeline 2011 and the return branch pipe 2012 in sequence to achieve self-circulation flow, so as to ensure that the sterilization device 204 completely kills bacteria in the water. A water quality sterilization timer device can be set in the sterilization chamber 201 as needed. When the water quality sterilization time meets the requirements, the water quality sterilization timer device sends a signal to the control system. The control system controls the control valve 206 to rotate to achieve the conversion. At this time, the return branch pipe 2012 is closed and the first output branch pipe 2013 is opened. The water in the sterilization chamber 201 flows into the regulating chamber 202. When the control valve 206 is turned to the middle position, both the return branch pipe 2012 and the first output branch pipe 2013 are closed. The water sterilization timing device and control valve 206 are both technologies known in the art and are commercially available products.

[0036] like Figure 1As shown, the carbon dioxide regulation system 2022 of the regulating chamber 202 includes a first gas stone 211, a pH detection probe 210, a carbon dioxide tank 219, a controller 209, and a solenoid valve 220. The first gas stone 211 is located on the bottom surface inside the regulating chamber 202, the pH detection probe 210 is located on the top cover of the regulating chamber 202, and the carbon dioxide tank 219, the controller 209, and the solenoid valve 220 are all located outside the regulating chamber 202. The first gas stone 211 is connected to the carbon dioxide tank 219 through a carbon dioxide input pipeline, and the solenoid valve 220 is provided on the carbon dioxide input pipeline. The solenoid valve 220 is controlled to open and close by the controller 209. In operation, the pH detection probe 210 continuously monitors the pH value of the water in the regulating chamber 202. When the pH value does not meet the requirements, the pH detection probe 210 sends a signal to the controller 209, which then controls the solenoid valve 220 to open, allowing carbon dioxide to be introduced into the regulating chamber 202 for adjustment. When the pH value meets the requirements, the pH detection probe 210 sends a signal to the controller 209 to close the solenoid valve 220. This invention considers that directly introducing carbon dioxide into the cultivation chamber 203 would cause drastic fluctuations in the pH value, which would affect the cultivation of Sargassum strain 107. Therefore, this invention includes a separate regulating chamber 202 to regulate the pH value. Only when both the pH value and water temperature meet the requirements is the second submersible pump 212 at one end of the second pipeline 2021 in the regulating chamber 202 activated to introduce water into the cultivation chamber 203. In this embodiment, the first gas stone 211 is a nano oxygen-enriching atomizing gas disc, which is a commercially available product. It can make the bubbles denser, smaller and more uniformly dissolved, saving gas consumption. In addition, the pH detection probe 210, controller 209 and solenoid valve 220 are all technologies known in the art and are commercially available products.

[0037] like Figure 1 As shown, in one embodiment of the present invention, the regulating chamber 202 is provided with a first temperature regulating system 2023, and the culture chamber 203 is provided with a second temperature regulating system 2032. The first temperature regulating system 2023 and the second temperature regulating system 2032 have the same structure, both including a heat exchange jacket 208 and a heat exchanger 2081. The heat exchange jacket 208 is provided with a heat exchange pipe 2082, which is connected to the heat exchanger 2081. The heat exchange pipe 2082 regulates the water temperature in the pipe through the heat exchanger 2081 and exchanges heat with the water in the chamber to achieve the purpose of water temperature regulation. In addition, a water temperature sensor is provided in the chamber to detect the water temperature in real time. When the water temperature does not meet the requirements, the heat exchanger 2081 is activated and exchanges heat with the water in the chamber through the heat exchange pipe 2082 in the heat exchange jacket 208 to achieve water temperature regulation. The heat exchanger 2081 and the water temperature sensor are both technologies known in the art and are commercially available products.

[0038] like Figure 1 As shown, in this embodiment, separate temperature control systems are installed in the regulating chamber 202 and the cultivation chamber 203, respectively. On the one hand, this allows for more precise control of the water temperature in the two chambers, ensuring accurate pH adjustment in the regulating chamber 202 and accurate temperature control for algae cultivation in the cultivation chamber 203. On the other hand, it facilitates the separation and combination of the two chambers. When separated, the temperature control system can move along with the corresponding chamber, and the regulating chamber 202 and the cultivation chamber 203 can operate independently after separation. The first temperature control system 2023, along with the controller 209 and other devices in the carbon dioxide regulation system 2022, can be integrated on the first frame on the top cover of the regulating chamber 202, while the second temperature control system 2032, the lighting system 2033, and other devices can be integrated on the second frame on the top cover of the cultivation chamber 203. In this way, the two chambers can be moved independently as a whole and can operate independently.

[0039] like Figure 1 As shown, in this embodiment, the second pipeline 2021 is provided with a second output branch pipe 2024 at its upper end, and the second output branch pipe 2024 is located at the upper end of the culture chamber 203. The first output branch pipe 2013 at the upper end of the first pipeline 2011 and the second output branch pipe 2024 at the upper end of the second pipeline 2021 can facilitate the disassembly and assembly of each chamber. When disassembling and assembling each chamber, it is not necessary to disassemble and install the pipeline separately. During assembly, it is only necessary to ensure that the first output branch pipe 2013 is inserted into the through hole of the top cover of the adjustment chamber 202, and the second output branch pipe 2024 is inserted into the through hole of the top cover of the culture chamber 203, so as to realize the water transfer of each chamber.

[0040] like Figure 2 As shown, in another embodiment of the present invention, the temperature regulation system includes a heat exchanger 2081 and a circulation pipe 221, wherein one end of the circulation pipe 221 is located in the regulation chamber 202 and the other end is located in the culture chamber 203. The circulation pipe 221 is equipped with a circulation pump and the heat exchanger 2081. The circulation pipe 221 realizes the circulation of water within the regulation chamber 202 and the culture chamber 203, specifically as follows: Figure 2As shown, the second submersible pump 212 drives the water in the regulating chamber 202 to the cultivation chamber 203, while the circulation pump on the circulation pipeline 221 drives the water in the cultivation chamber 203 to the regulating chamber 202. The circulating water temperature is regulated when it passes through the heat exchanger 2081, which ensures that the water temperature and pH value in the regulating chamber 202 and the cultivation chamber 203 are consistent. Since the pH value of the water will change during the growth of algae, this embodiment can adjust the parameter values ​​in the two chambers in real time. The water circulation speed is adjusted by the cooperation of the second submersible pump 212 and the circulation pump.

[0041] like Figure 1 As shown, the lighting system 2033 above the culture chamber 203 includes a light lamp 215 and a light controller 216. In this embodiment, the light lamp 215 is an LED lamp, and its light intensity is controlled and adjusted by the light controller 216. The light controller 216 is a technology known in the art and is a commercially available product. Furthermore, the walls and top cover of the adjustment chamber 202 and the culture chamber 203 are made of transparent acrylic material to ensure adequate lighting.

[0042] like Figure 1 As shown, the oxygen regulation system 2031 includes a second gas stone 213 and an air pump 214. The second gas stone 213 is located on the bottom surface of the culture chamber 203 and is connected to the air pump 214 through an oxygen input pipeline. An oxygen concentration sensor is installed in the culture chamber 203 to detect the oxygen status in real time. When the detected value does not meet the requirements, the oxygen concentration sensor sends a signal to the control system, and the control system controls the air pump 214 to start. The oxygen concentration sensor and the air pump 214 are both technologies known in the art and are commercially available products. In addition, the second gas stone 213 is a nano oxygen-enhancing atomizing disc.

[0043] like Figure 1 As shown, a wave pump 218 is provided on one side of the culture chamber 203 to simulate seawater flow. The wave pump 218 is a commercially available product.

[0044] like Figure 3 As shown, the fixation substrate 1 in the culture chamber 203 includes a fixation substrate base 101, and the fixation substrate base 101 is provided with a telescopic rod 103 and a support rod 102. The lower end of the Sargassum algae strain 107 is fixed to the upper end of the telescopic rod 103 by a fixing wire 106. The detection optical fiber 104 is fixed to the support rod 102, and the detection probe of the detection optical fiber 104 is aligned with the Sargassum algae strain 107. The detection optical fiber 104 is externally connected to a chlorophyll fluorometer to realize in-situ measurement of the photosynthetic activity of the algae. The chlorophyll fluorometer is a commercially available product. Each algae strain in the culture chamber 203 needs to be equipped with a detection optical fiber 104.

[0045] like Figure 3 As shown, the telescopic rod 103 includes multiple threaded sleeves 1031 connected sequentially from top to bottom. The operator can adjust the overall height of the telescopic rod 103 by screwing each threaded sleeve 1031 to match the growth of the algae. A sliding sleeve 105 is fitted on the support rod 102, and a locking screw 1051 is provided on the sliding sleeve 105. The support rod 102 has multiple screw holes. The detection optical fiber 104 is fixed to the sliding sleeve 105. By loosening the locking screw 1051, the sliding sleeve 105 can move up and down along the support rod 102 to adjust its height, that is, to adjust the height of the detection optical fiber 104. After the height of the sliding sleeve 105 is determined, the operator tightens the locking screw 1051 to make it threadedly connected with the corresponding screw hole on the support rod 102 to lock the position of the sliding sleeve 105.

[0046] like Figure 3 As shown, the fixation base 101 is a regular cube and is made of thermoplastic free resin that is easy for algal spores to attach to. The fixation base 101 is provided with regular grid lines 108 to facilitate the counting of algal spore attachment density. The fixation base 101 is provided with a number mark 109 on one side to facilitate the numbering of test algal strains.

[0047] Furthermore, this invention allows for the parallel connection and combination of multiple incubator units 2 according to actual needs, such as... Figure 1 As shown, at this time, the water inlet 207 of each incubator unit 2 is connected to a main water inlet pipe.

[0048] The working principle of this invention is as follows:

[0049] When the system of this invention is working, such as Figures 1-2 As shown, water first enters the sterilization chamber 201 through the inlet 207. At this time, the return branch pipe 2012 at the upper end of the first pipeline 2011 is opened and the first output branch pipe 2013 is closed. The water in the chamber undergoes multiple self-circulation flows to ensure thorough sterilization. Only when the sterilization time of the water in the sterilization chamber 201 meets the requirements will the control valve 206 at the upper end of the first pipeline 2011 be rotated to achieve the conversion. At this time, the return branch pipe 2012 is closed and the first output branch pipe 2013 is opened. The water in the sterilization chamber 201 is then input into the regulating chamber 202 through the action of the first submersible pump 205.

[0050] The regulating chamber 202 is used to regulate parameters such as pH and temperature of the water. A pH detection probe 210 at the upper end of the regulating chamber 202 monitors the pH value of the water inside the chamber in real time. When the pH value does not meet the requirements, the pH detection probe 210 sends a signal to the controller 209. The controller 209 then controls the solenoid valve 220 to open, allowing carbon dioxide to enter the regulating chamber 202 for regulation. Simultaneously, the first temperature regulation system 2023 within the regulating chamber 202 regulates the water temperature. Only when both the pH value and water temperature meet the requirements is the second submersible pump 212 within the regulating chamber 202 activated to output water to the cultivation chamber 203. This invention uses a separate regulating chamber 202 to prevent direct introduction of carbon dioxide into the cultivation chamber 203, which would cause drastic fluctuations in the pH value and negatively impact the cultivation of Sargassum strain 107.

[0051] The cultivation chamber 203 is equipped with a fixation substrate 1 to fix Sargassum algae strain 107, and a detection light 104 is provided on the fixation substrate 1 to connect to a chlorophyll fluorometer to realize in-situ measurement of photosynthetic activity of algae. At the same time, the illumination lamp 215 above the cultivation chamber 203 is adjusted by a light controller 216 to adjust the illumination. The oxygen regulation system 2031 in the cultivation chamber 203 adjusts the oxygen supply according to the algae strain and water conditions.

[0052] Other examples Figure 1 As shown, the present invention can separately install temperature control systems in the regulating chamber 202 and the culture chamber 203 to adjust the water temperature according to actual conditions. This embodiment facilitates the disassembly and independent operation of the various parts of the invention, such as... Figure 2 As shown, the present invention can also utilize the circulation pipe 221 to achieve water circulation in the regulating chamber 202 and the cultivation chamber 203, and achieve water temperature regulation during the circulation process. This embodiment can be adjusted in real time according to the growth of the algae. The present invention can ensure a suitable growth environment for the Sargassum algae strain 107. At the same time, the cultivation chamber 203 of the present invention has sufficient volume to meet the growth needs of large Sargassum algae. Furthermore, the present invention can also use multiple cultivation chamber units 2 simultaneously for algae cultivation.

[0053] The cultivation method of the present invention includes the following steps:

[0054] Step 1: Algae treatment. After collecting the main stem of Sargassum with newly branched branches, soak it in seawater potassium iodide solution for a set time to kill small and medium-sized animals, insect eggs, etc. attached to the algae. Then clean the surface of the algae and remove the attached miscellaneous algae and dirt. Place the cleaned algae in a glass container and ventilate for temporary cultivation. After a set number of days of cultivation, clean the surface of the algae again. This invention can effectively inhibit the attachment and growth of common miscellaneous algae such as filamentous algae, shell-shaped coral algae, and diatoms through secondary algae treatment.

[0055] Taking Sargassum var. vannamei culture as an example: the main stem of Sargassum var. vannamei (with primary branches) is collected at low tide. Since Sargassum var. vannamei is a perennial algae, its main stem is often attached with small algae or microalgae such as diatoms, as well as many animals such as sandworms. Therefore, after collection, it is soaked in a 0.7% potassium iodide solution for 30 minutes to kill the small and medium-sized animals and insect eggs attached to the algae. Then, the attached algae and dirt on the surface of the algae are cleaned with a brush, blade and other tools. After that, it is placed in a glass tank for temporary cultivation with ventilation. The cultivation temperature is 20℃ and the light is natural light. After 7 to 10 days of cultivation, the surface of the algae is cleaned again with a brush, blade and other tools.

[0056] Step 2: Treatment of incubator unit 2 before algal culture. Disinfect each compartment of incubator unit 2 with disinfectant (84 solution).

[0057] Taking Sargassum fusiforme culture as an example: each chamber of incubator unit 2 is disinfected with 84 solution. After soaking for 24 hours with 10 mL of 84 solution per 1 L of seawater (100:1), it is then soaked in clean filtered seawater for another 24 hours to achieve the effect of sterilization and disinfection.

[0058] Step 3: Fixing the algal plants. The Sargassum main stem is divided into a set number of equal parts, each part including the main stem and primary branches. Then, each part of the algal plant is fixed to the upper end of the telescopic rod 103 on the corresponding anchoring base 1 in the cultivation chamber 203 using fixing wire 106 (electroplated galvanized iron wire). As the algal plants grow, the growth height of the algal plants in the cultivation chamber 3 can be adjusted by telescopic rod 103 to ensure that they receive the same light intensity.

[0059] Step 4: Determine the range of each parameter in incubator unit 2 and monitor and control them in real time during algae cultivation. The parameters of incubator unit 2 include water quality, water pH, water temperature, water oxygen concentration, light intensity, and light cycle. Water quality is controlled by sterilization chamber 201, water pH is controlled by carbon dioxide regulation system 2022 in regulation chamber 202, water temperature is controlled by temperature regulation system, water oxygen concentration is controlled by oxygen regulation system 2031 in cultivation chamber 203, and light intensity and light cycle are controlled by light system 2033 above cultivation chamber 203.

[0060] During algal culture, water should be changed regularly and water quality parameters should be recorded regularly. Taking Sargassum fusiforme culture as an example: light intensity is set to 4000 lux, seawater salinity is 30-32, water pH is 8.10-8.25, half of the water is changed every 5 days, and a culture cycle is 10 days. Every 10 days, all the water is changed and the culture container and control device are washed.

[0061] like Figures 4-5As shown, after 30 days of cultivation at 22℃, the algal strains of this invention showed normal growth and development, with lush foliage, long inter-leaf spacing, and the development of air sacs, achieving a daily average relative growth rate of 2.84%. In contrast, the control group, cultured in 500mL open bottles (400mL water) in a 22℃ constant temperature incubator, exhibited abnormal growth and development, characterized by small, curled leaves, short inter-leaf spacing, a dwarf-like appearance, and the absence of air sacs, with a daily average relative growth rate of only 0.12%. Furthermore, as shown... Figure 6 As shown, the photosynthetic activities of the two also show significant differences.

Claims

1. An indoor closed-loop Sargassum culture system, characterized in that: The system includes an incubator unit (2), which comprises a sterilization chamber (201), a regulating chamber (202), an incubation chamber (203), and a temperature control system. The sterilization chamber (201) is an opaque chamber. An inlet (207) is located at the upper end of the sterilization chamber (201). A sterilization device (204) and a first pipeline (2011) are located inside the sterilization chamber (2011). A return branch pipe (2012), a first output branch pipe (2013), and a control valve (206) are located at the upper end of the first pipeline (2011). The return branch pipe (2012) is located at the upper end of the sterilization chamber (201), and the first output branch pipe (2013) is located at the upper end of the regulating chamber (202). The return branch pipe (2012) and the first output branch pipe (2013) are connected by the control valve (206). The control valve (206) controls the switching. The regulating chamber (202) is equipped with a second pipeline (2021) and a carbon dioxide regulating system (2022). The second pipeline (2021) is connected to the culture chamber (203). The pH value of the water is regulated by controlling the carbon dioxide input through the carbon dioxide regulating system. The culture chamber (203) is equipped with a fixation base (1) and an oxygen regulating system (2031). A light system (2033) is provided above the culture chamber (203). A drain outlet (217) is provided on the lower side of one end of the culture chamber (203). A height-adjustable telescopic rod (103) is provided on the fixation base (1), and the Sargassum algae strain (107) is placed on the telescopic rod (103). The water temperature of the regulating chamber (202) and the water temperature of the culture chamber (203) are both regulated by the temperature regulating system.

2. The indoor closed-loop Sargassum culture system according to claim 1, characterized in that: The sterilization device (204) is an ultraviolet sterilization lamp, the first pipeline (2011) is equipped with a first submersible pump (205), and the sterilization chamber (201) is equipped with a water quality sterilization timer.

3. The indoor closed-loop Sargassum culture system according to claim 1, characterized in that: The carbon dioxide regulation system (2022) includes a first gas stone (211), a pH detection probe (210), a carbon dioxide tank (219), a controller (209), and a solenoid valve (220). The first gas stone (211) is located on the bottom surface inside the regulation chamber (202), and the pH detection probe (210) is located on the top cover of the regulation chamber (202). The carbon dioxide tank (219), the controller (209), and the solenoid valve (220) are all located outside the regulation chamber (202). The first gas stone (211) is connected to the carbon dioxide tank (219) through a carbon dioxide input pipeline. The carbon dioxide input pipeline is equipped with a solenoid valve (220), and the solenoid valve (220) is controlled to open and close by the controller (209).

4. The indoor closed-loop Sargassum culture system according to claim 1, characterized in that: The regulating chamber (202) is provided with a first temperature regulating system (2023), and the culture chamber (203) is provided with a second temperature regulating system (2032). The first temperature regulating system (2023) and the second temperature regulating system (2032) have the same structure, both including a heat exchange jacket (208) and a heat exchanger (2081). The heat exchange jacket (208) is provided with a heat exchange pipeline (2082), and the heat exchange pipeline (2082) is connected to the heat exchanger (2081). The lower end of the second pipeline (2021) is provided with a second submersible pump (212), and the upper end is provided with a second output branch pipe (2024). The second output branch pipe (2024) is located at the upper end of the culture chamber (203).

5. The indoor closed-loop Sargassum culture system according to claim 1, characterized in that: The temperature control system includes a heat exchanger (2081) and a circulation pipeline (221), wherein one end of the circulation pipeline (221) is located in the control chamber (202) and the other end is located in the culture chamber (203). The circulation pipeline (221) is equipped with a circulation pump and a heat exchanger (2081), and the lower end of the second pipeline (2021) is equipped with a second submersible pump (212).

6. The indoor closed-loop Sargassum culture system according to claim 1, characterized in that: The lighting system (2033) includes a lamp (215) and a light controller (216), and the light intensity of the lamp is controlled and adjusted by the light controller (216).

7. The indoor closed-loop Sargassum culture system according to claim 1, characterized in that: The oxygen regulation system (2031) includes a second gas stone (213) and an air pump (214). The second gas stone (213) is located on the bottom surface of the culture chamber (203) and is connected to the air pump (214) through an oxygen input pipeline. An oxygen concentration sensor is provided in the culture chamber (203).

8. The indoor closed-loop Sargassum culture system according to claim 1, characterized in that: The fixation substrate (1) in the culture chamber (203) includes a fixation substrate base (101), and the fixation substrate base (101) is provided with a telescopic rod (103) and a support rod (102). The lower end of the Sargassum algae strain (107) is fixed to the upper end of the telescopic rod (103) by a fixing wire (106). The detection optical fiber (104) is fixed to the support rod (102), and the detection probe of the detection optical fiber (104) is aligned with the Sargassum algae strain (107). The detection optical fiber (104) is externally connected to a chlorophyll fluorometer.

9. The indoor closed-loop Sargassum culture system according to claim 8, characterized in that: The telescopic rod (103) includes a plurality of threaded sleeves (1031) connected sequentially from top to bottom; a sliding sleeve (105) is fitted on the support rod (102), and a locking screw (1051) is provided on the sliding sleeve (105); a plurality of screw holes are provided on the support rod (102); the detection optical fiber (104) is fixed on the sliding sleeve (105); and after the height of the sliding sleeve (105) is determined, the locking screw (1051) is threaded into the corresponding screw hole.

10. An indoor closed-loop Sargassum culture system according to claim 1, characterized in that: Includes the following steps: Step 1: Algae treatment. After collecting the main stem of Sargassum with initial branches, soak it in seawater potassium iodide solution for a set time to kill small and medium-sized animals, insect eggs, etc. attached to the algae. Then clean the surface of the algae and remove the attached miscellaneous algae and dirt. Place the cleaned algae in a glass container and ventilate for temporary cultivation. After a set number of days of temporary cultivation, clean the surface of the algae again. Step 2: Treatment of the incubator unit (2) before algal culture: Disinfect the incubator unit (2) with disinfectant. Step 3: Fixing the algal strains. The main stem of Sargassum is divided into a set number of equal parts, each part including the main stem and the initial branches. Then, each part of the algal strain is fixed to the upper end of the telescopic rod (103) on the corresponding anchoring base (1) in the culture chamber (203). Step 4: Determine the range of each parameter of the incubator unit (2) and monitor and control it in real time during algae cultivation. The parameters of the incubator unit (2) include water quality, water pH value, water temperature, water oxygen concentration, light intensity and light cycle. Among them, water quality is controlled by the sterilization chamber (201), water pH value is controlled by the carbon dioxide regulation system (2022) of the regulation chamber (202), water temperature is controlled by the temperature regulation system, water oxygen concentration is controlled by the oxygen regulation system (2031) of the cultivation chamber (203), and light intensity and light cycle are controlled by the light system (2033) above the cultivation chamber (203). During algae cultivation, the water is changed regularly and the water quality parameters are recorded regularly.

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