Production process and application of traditional Chinese medicine granules containing astragalus, chrysanthemum and monk fruit

Abstract

The invention belongs to the field of traditional Chinese medicine, and specifically relates to a production process and application of a traditional Chinese medicine granule containing astragalus, chrysanthemum and monk fruit. The invention adds water to astragalus, chrysanthemum and monk fruit according to a mass ratio of 1: (0.2-0.8): (0.4-0.9), stirs and extracts, then uses protease to enzymolyze, and then inoculates fermentation bacteria composed of live bacteria of fermented lactobacillus GDMCC 1.1796 and Bifidobacterium breve CICC 6185 according to a quantity ratio of 1: 1.5-3, and adjusts the content of the composite bacteria in the mixed material to 0.5×10 7 ‑2×10 7 cfu / mL, fermentation, then adding ethanol to adjust the ethanol concentration to 20-35wt%, further extraction, post-treatment, to obtain the Chinese medicine granules of the present invention. The process of the present invention improves the efficacy of Chinese medicine granules, has excellent antioxidant effect, and can also significantly improve the immune function of the human body.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and particularly relates to a production process and application of a traditional Chinese medicine granule containing astragalus, chrysanthemum and momordica grosvenori. Background Art

[0002] Chinese medicine granules are granules made from the extraction and concentration of Chinese medicine slices for use in clinical prescriptions of traditional Chinese medicine. They were formerly known as single-ingredient concentrated Chinese medicine granules, and their trade names and folk names include decoction-free Chinese medicine slices, new slices, refined slices, beverage-type slices, scientific Chinese medicine, etc. Chinese medicine granules are made from traditional Chinese medicine slices, and are processed into prescription medicines through production processes such as extraction, separation, concentration, drying, granulation, and packaging.

[0003] Astragalus, chrysanthemum and monk fruit are common Chinese medicine raw materials with immunity-enhancing and antioxidant effects. When they are processed, it is often not conducive to the efficacy of the above Chinese medicine raw materials. Therefore, providing a production process to increase the efficacy of astragalus, chrysanthemum and monk fruit, and obtaining a Chinese medicine granule that can effectively enhance immunity and antioxidant effects is a technical problem that needs to be solved at present. Summary of the invention

[0004] The present invention is proposed in view of the above technical problems. The present invention belongs to the field of traditional Chinese medicine, and specifically relates to a production process and application of traditional Chinese medicine granules containing astragalus, chrysanthemum and monk fruit.

[0005] In one aspect, the present invention provides a production process of a Chinese medicine granule containing astragalus, chrysanthemum and momordica grosvenori, comprising the following steps:

[0006] Step 1, selecting dried astragalus, chrysanthemum and monk fruit, and crushing them according to a mass ratio of 1: (0.2-0.8): (0.4-0.9) to obtain a crushed material;

[0007] Step 2, mixing the crushed material with water in a mass ratio of 1:20-100, heating and stirring to obtain a mixture;

[0008] Step 3, cooling the mixture, adding protease to obtain a premix, performing enzymolysis, inactivating the enzyme and sterilizing to obtain an enzymolyzate;

[0009] Step 4, inoculating the enzymatic hydrolysate with composite bacteria to obtain a mixed material, fermenting it, and obtaining a fermentation product;

[0010] Step 5, sterilizing the fermented product, adding ethanol, adjusting the ethanol concentration to obtain an ethanol material, and then stirring to obtain a stirred material;

[0011] Step 6: The stirred material is filtered, concentrated, freeze-dried, and crushed to obtain Chinese medicine granules.

[0012] Furthermore, in step 1, the crushing is performed to a particle size of less than 5 mm.

[0013] Furthermore, in step 2, the heating temperature is 50-70°C.

[0014] Furthermore, in step 2, the stirring speed is 50-500 rpm, and the stirring time is 3-20 hours.

[0015] Further, in step 3, the temperature is lowered to 30-45°C.

[0016] Furthermore, in step 3, the protease is papain.

[0017] Furthermore, in step 3, the protease content in the premix is ​​800-4000 U / g.

[0018] Furthermore, in step 3, the enzymolysis temperature is 30-45° C. and the time is 4-15 hours.

[0019] Furthermore, in step 4, the composite bacteria are Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CICC 6185 in a live bacteria ratio of 1:1.5-3.

[0020] Further, in step 4, the content of the composite bacteria in the mixed material is 0.5×10 7 -2×10 7 cfu / mL.

[0021] Furthermore, in step 4, the fermentation is anaerobic fermentation.

[0022] Furthermore, in step 4, the fermentation temperature is 28-38° C. and the fermentation time is 8-14 hours.

[0023] Furthermore, in step 5, the concentration of ethanol in the stirred material is 20-35 wt %.

[0024] Furthermore, in step 5, the stirring temperature is 40-60° C., the time is 5-10 hours, and the rotation speed is 40-400 rpm.

[0025] Further, in step 6, filtering is performed using a filter membrane with a pore size of 0.2-5 microns.

[0026] Furthermore, in step 6, the concentration is carried out under reduced pressure, and the concentration temperature is 55-60°C.

[0027] Further, in step 6, the concentrate is concentrated to 10-20% of the mass of the foreign teacher class material.

[0028] Further, in step 6, the freeze-drying temperature is -50 to -25°C.

[0029] Further, in step 6, freeze-drying is performed until the water content is ≤8.27%.

[0030] Furthermore, the production process of the Chinese medicine granules containing astragalus, chrysanthemum and monk fruit comprises the following steps:

[0031] Step 1, selecting dried astragalus, chrysanthemum and monk fruit, and crushing them according to a mass ratio of 1: (0.2-0.8): (0.4-0.9) to obtain a crushed material;

[0032] Step 2, mixing the crushed material with water in a mass ratio of 1:20-100, heating to 50-70° C. and stirring for 3-20 hours to obtain a mixture;

[0033] Step 3, cooling the mixture, adding papain and adjusting the content to 800-4000 U / g to obtain a premix, performing enzymolysis at 30-45° C. for 4-15 hours, inactivating the enzyme and sterilizing to obtain an enzymolyzate;

[0034] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:1.5-3 to obtain a mixed material. The bacterial content in the mixed material was 0.5×10 7 -2×10 7 cfu / mL, anaerobic fermentation at 28-38°C for 8-14 hours to obtain a fermentation product;

[0035] Step 5, sterilizing the fermented product, adding ethanol, adjusting the ethanol concentration to 20-35wt%, obtaining an ethanol material, and then stirring at a temperature of 40-60° C. for 5-10 hours to obtain a stirred material;

[0036] Step 6: The stirred material is filtered, concentrated, freeze-dried, and crushed to obtain Chinese medicine granules.

[0037] On the other hand, the present invention also provides the Chinese medicine granules produced by the production process.

[0038] On the other hand, the present invention also provides the use of the Chinese medicine granules produced by the production process in the preparation of Chinese medicine preparations.

[0039] The beneficial effects of the present invention are as follows:

[0040] The invention provides a method for preparing a mixture of astragalus, chrysanthemum and monk fruit in a mass ratio of 1:(0.2-0.8):(0.4-0.9), adding water, stirring and leaching, and then using protease for enzymolysis, and then inoculating fermentation bacteria composed of fermented lactobacillus GDMCC 1.1796 and bifidobacterium breve CICC 6185 live bacteria in a quantity ratio of 1:1.5-3, and adjusting the content of the composite bacteria in the mixed material to 0.5×10 7 -2×10 7cfu / mL, fermentation is carried out, and then ethanol is added to adjust the ethanol concentration to 20-35wt%, further extraction and post-treatment are carried out to obtain the traditional Chinese medicine granules of the present invention.

[0041] The process of the present invention improves the efficacy of traditional Chinese medicine granules, has excellent antioxidant effect, and can also significantly enhance the immune function of the human body.

[0042] The present invention uses fermented Lactobacillus GDMCC 1.1796 and Bifidobacterium breve CICC 6185 for fermentation, and the two together promote the extraction and effect of active ingredients, and mutually promote the antioxidant and human immunity effects of the particles. The ratio of the two bacteria is within the range of 1.5-3:1, which can significantly improve the effect of the particles.

[0043] The invention adopts papain to improve the antioxidant and immunity-enhancing effects of the particles to a certain extent.

[0044] In order to further improve the efficacy of the particles, the present invention further extracts the particles using ethanol-water solution. In particular, when the ethanol concentration in the mixed solution is in the range of 20-35wt%, the antioxidant and immunity-enhancing properties of the particles are significantly improved. DETAILED DESCRIPTION

[0045] The technical solution of the present invention will be explained below in conjunction with specific implementation methods, and the effect of the technical solution of the present invention will be clearer and more specific.

[0046] Lactobacillus fermentum GDMCC 1.1796: Limosilactobacillus fermentum, GDMCC NO.: 1.1796, Guangdong Provincial Microbiological Culture Collection Center.

[0047] Bifidobacterium breve CI CC 6185: Bifidobacterium breve, number China Industrial Microorganism Culture Collection Management Center.

[0048] Lactobacillus reuteri CI CC 6226: Limosilactobacillus reuteri, number China Industrial Microorganism Culture Collection Management Center.

[0049] Lactobacillus plantarum CI CC 21791: Lactiplantibacillus plantarum, number China Industrial Microorganism Culture Collection Management Center.

[0050] 1. Preparation of Particles

[0051] Granule 1:

[0052] The production process is as follows:

[0053] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0054] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0055] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0056] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:2.2 to obtain a mixed material. The fermentation bacteria content in the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0057] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0058] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 1.

[0059] Particle 2:

[0060] The production process is as follows:

[0061] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.4:0.7 to a particle size of less than 5 mm to obtain a crushed material;

[0062] Step 2, mixing the crushed material with water in a mass ratio of 1:55, heating to 60° C., stirring at 70 rpm for 6.9 hours to obtain a mixture;

[0063] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1200U / g to obtain a premix, and enzymolysis is performed at 34°C for 7.2 hours, the enzyme is inactivated and sterilized to obtain an enzymolyzate;

[0064] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:3 to obtain a mixed material. The fermentation bacteria content in the mixed material was 9.7×10 7 cfu / mL, followed by anaerobic fermentation at 30°C for 8.5 hours to obtain a fermentation product;

[0065] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 22wt%, obtaining an ethanol material, and then stirring at 50°C and 70rpm for 8 hours to obtain a stirred material;

[0066] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -30° C. to a water content of about 5.18%, and crushed to obtain Chinese medicine granules 2.

[0067] Particle 3:

[0068] The production process is as follows:

[0069] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0070] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0071] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0072] Step 4: The enzymatic hydrolysate is inoculated with fermentation bacteria Lactobacillus reuteri CICC 6226 to obtain a mixed material, wherein the fermentation bacteria content in the mixed material is 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0073] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0074] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 3.

[0075] Particle 4:

[0076] The production process is as follows:

[0077] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0078] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0079] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0080] Step 4: Inoculate the enzymatic hydrolysate with fermentation bacteria Bacillus plantarum CICC 21791 to obtain a mixed material, wherein the content of fermentation bacteria in the mixed material is 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0081] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0082] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 4.

[0083] Particle 5:

[0084] The production process is as follows:

[0085] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0086] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0087] Step 3, the mixture is cooled to 35°C, alkaline protease is added and the content is adjusted to 1000U / g to obtain a premix, and enzymatic hydrolysis is performed at 35°C for 6.5 hours, the enzyme is inactivated and sterilized to obtain an enzymolyzate;

[0088] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:2.2 to obtain a mixed material. The content of the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0089] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0090] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 5.

[0091] Particle 6:

[0092] The production process is as follows:

[0093] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0094] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0095] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0096] Step 4: The enzymatic hydrolysate was inoculated with Lactobacillus fermentum GDMCC 1.1796 to obtain a mixed material, wherein the content of fermentation bacteria in the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0097] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0098] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 6.

[0099] Particle 7:

[0100] The production process is as follows:

[0101] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0102] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0103] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0104] Step 4: The enzymatic hydrolysate was inoculated with Bifidobacterium breve CICC 6185 to obtain a mixed material, wherein the fermentation bacteria content in the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0105] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0106] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 7.

[0107] Particle 8:

[0108] The production process is as follows:

[0109] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0110] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0111] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0112] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:0.8 to obtain a mixed material. The content of the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0113] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0114] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 8.

[0115] Particle 9:

[0116] The production process is as follows:

[0117] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0118] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0119] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0120] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:4.5 to obtain a mixed material. The content of the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0121] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 25.8 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0122] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 9.

[0123] Granule 10:

[0124] The production process is as follows:

[0125] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0126] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0127] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0128] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:2.2 to obtain a mixed material. The content of the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0129] Step 5, sterilizing the fermented product, adding ethanol, adjusting the ethanol concentration to 10 wt%, obtaining an ethanol material, and then stirring at 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0130] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 10.

[0131] Particle 11:

[0132] The production process is as follows:

[0133] Step 1, select dried astragalus, chrysanthemum and monk fruit, and crush them according to the mass ratio of 1:0.5:0.8 to a particle size of less than 5 mm to obtain a crushed material;

[0134] Step 2, mixing the crushed material with water in a mass ratio of 1:50, heating to 62° C., stirring at 70 rpm for 8 hours to obtain a mixture;

[0135] Step 3, the mixture is cooled to 35°C, papain is added and the content is adjusted to 1000U / g to obtain a premix, and enzymolysis is performed at 35°C for 6.5 hours to inactivate the enzyme and sterilize to obtain an enzymolyzate;

[0136] Step 4: The enzymatic hydrolysate was inoculated with a composite bacteria consisting of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CI CC 6185 in a ratio of 1:2.2 to obtain a mixed material. The content of the mixed material was 9.6×10 7 cfu / mL, and then anaerobic fermentation was performed at 32°C for 10 hours to obtain the fermentation product;

[0137] Step 5, sterilizing the fermented product, adding ethanol to adjust the ethanol concentration to 72 wt % to obtain an ethanol material, and then stirring at a temperature of 52° C. and 60 rpm for 7.2 hours to obtain a stirred material;

[0138] Step 6: The stirred material is filtered using a filter membrane with a pore size of 0.5 micron, concentrated under reduced pressure at 57.5° C., freeze-dried at -32° C. to a water content of about 5.27%, and crushed to obtain Chinese medicine granules 11.

[0139] 2. Application Testing

[0140] 1. Antioxidant effect test

[0141] The EC50 value of the drug concentration corresponding to the scavenging of 50% free radicals (DPPH, ABTS) by the aqueous solution of the above different Chinese herbal granules is used to characterize the antioxidant effect of the Chinese herbal granules.

[0142] The free radical scavenging effect was tested using a DPPH kit and an ABTS kit, which were sourced from Suzhou Keming Biotechnology Co., Ltd.

[0143] The specific method is: use deionized water to prepare aqueous solutions of Chinese medicine granules of different concentrations, use DPPH kit and ABTS kit to test the free radical scavenging rate of aqueous solutions of Chinese medicine granules of different concentrations, draw a standard curve, and then determine the EC50 value (unit mg / mL) of each group of Chinese medicine granules. The specific test results of the antioxidant effect are shown in Table 1.

[0144] Table 1 Antioxidant effect

[0145]

[0146] According to the test data in Table 1, the ratio of the EC50 concentration increase of each group compared with that of particle 1 was calculated, and the results are shown in Table 2.

[0147]

[0148]

[0149] 2. Test on the effect of improving immunity

[0150] Experimental method: SPF male Balb / c mice weighing 20±2g were randomly divided into 12 groups, a control group and a granule 1-11 group, with 10 mice in each group. The granule 1-11 group was gavaged with 20mL of granule solution / kg mouse per day. The granule solution was a saline solution of the above-mentioned granules 1-11 (concentration of 0.1g / mL). The control group was gavaged with the same amount of saline in the same manner. After one month of gavage, each group of mice was intraperitoneally injected with 0.2mL of 2wt% SRBC for immunization. Then gavage was continued for 4 days, and the mice in each group were killed. The spleen was removed, placed in Hank's solution, ground into a suspension, filtered through 200 mesh, centrifuged at 1000r / min for 10min, washed with Hank's solution, and the cells were suspended in RPMI 1640 culture medium, the cells were counted, and the cell concentration was adjusted to 5×106 / mL spleen cell suspension.

[0151] The surface culture medium (1 g agarose plus distilled water to 100 mL) was heated to dissolve, kept warm in a 45°C water bath, mixed with an equal amount of Hank's solution with a pH of 7.3 and twice the concentration, and dispensed into test tubes, 0.5 mL per tube. 50 μL of 10% volume ratio SRBC (prepared with SA buffer) and 20 μL of spleen cell suspension (5×10 6 / mL), mix well, pour onto a glass slide containing a thin layer of agarose, make parallel slices, wait for the agar to solidify, put the glass slide horizontally on the slide rack, and place it in a 5% CO 2 Incubate in an incubator at 37°C for 1.5 hours, then dilute with SA buffer (1:8) and add to the groove of the slide rack, continue incubation at 37°C for 1.5 hours, and count the number of hemolytic plaques. The results are shown in Table 3.

[0152] Table 3 Immunity enhancement effect test

[0153]

[0154]

[0155] The invention provides a method for preparing a mixture of astragalus, chrysanthemum and monk fruit in a mass ratio of 1:(0.2-0.8):(0.4-0.9), adding water, stirring and leaching, and then using protease for enzymolysis, and then inoculating fermentation bacteria composed of fermented lactobacillus GDMCC 1.1796 and bifidobacterium breve CICC 6185 live bacteria in a quantity ratio of 1:1.5-3, and adjusting the content of the composite bacteria in the mixed material to 0.5×10 7 -2×10 7 cfu / mL, fermentation is carried out, and then ethanol is added to adjust the ethanol concentration to 20-35wt%, further extraction and post-treatment are carried out to obtain the traditional Chinese medicine granules of the present invention.

[0156] The process of the present invention improves the efficacy of traditional Chinese medicine granules, has excellent antioxidant effect, and can also significantly enhance the immune function of the human body.

[0157] The test results in Tables 1-3 show that the present invention uses Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CICC 6185 for fermentation, and the two together promote the extraction and effect of active ingredients, and mutually promote the antioxidant and human immunity effects of the particles. When the ratio of the two bacteria is within the range of 1.5-3:1, the particle effect can be significantly improved.

[0158] The invention adopts papain to improve the antioxidant and immunity-enhancing effects of the particles to a certain extent.

[0159] In order to further improve the efficacy of the particles, the present invention further extracts the particles using ethanol-water solution. In particular, when the ethanol concentration in the mixed solution is in the range of 20-35wt%, the antioxidant and immunity-enhancing properties of the particles are significantly improved.

[0160] Those skilled in the art may make various changes and modifications to the present invention. If these changes and modifications fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include these changes and modifications.

Claims

1. A production process of Chinese medicine granules containing astragalus, chrysanthemum and momordica grosvenori, characterized in that: The steps include: Step 1, selecting dried astragalus, chrysanthemum and monk fruit, and crushing them according to a mass ratio of 1: (0.2-0.8): (0.4-0.9) to obtain a crushed material; Step 2, mixing the crushed material with water in a mass ratio of 1:20-100, heating and stirring to obtain a mixture; Step 3, cooling the mixture, adding protease to obtain a premix, performing enzymolysis, inactivating the enzyme and sterilizing to obtain an enzymolyzate; Step 4, inoculating the enzymatic hydrolysate with composite bacteria to obtain a mixed material, fermenting it, and obtaining a fermentation product; Step 5, sterilizing the fermented product, adding ethanol, adjusting the ethanol concentration to obtain an ethanol material, and then stirring to obtain a stirred material; Step 6, filtering the stirred material, concentrating, freeze-drying, and crushing to obtain Chinese medicine granules; The composite bacteria consisted of Lactobacillus fermentum GDMCC 1.1796 and Bifidobacterium breve CICC 6185 with a live bacterial count ratio of 1:1.5-3; The content of composite bacteria in the mixed material is 0.5×10 7 -2×10 7 cfu / mL; The concentration of ethanol in the stirred material is 20-35wt%.

2. The process according to claim 1, characterized in that Step 2, the heating temperature is 50-70°C.

3. The process according to claim 1, characterized in that Step 3: the protease content in the premix is ​​800-4000 U / g.

4. The process according to claim 1, characterized in that The fermentation temperature is 28-38°C and the time is 8-14 hours.

5. The process according to claim 1, characterized in that Step 5, the stirring temperature is 40-60°C, the time is 5-10 hours, and the rotation speed is 40-400 rpm.

6. A Chinese medicine granule produced according to any one of the production processes of claims 1-5.

7. Use of the Chinese medicine granules according to claim 6 in the preparation of Chinese medicine preparations.

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