Intertidal zone organism sampling method and device

Abstract

The application relates to the technical field of biological sampling, and discloses an intertidal zone biological sampling method and device, which comprises a power plate, a top frame fixedly installed on the top of the power plate, a main handle fixedly installed on the rear side of the top frame, a moving block slidingly installed in the middle of the power plate, a piston cylinder fixedly installed above and below the moving block in the power plate, a connecting rod fixedly installed on the top of the moving block and the top of the piston cylinder, and a piston in the piston cylinder fixedly connected with the connecting rod. The micro air pump is controlled to draw air from below and exhaust air from above by pressing the operation button, so that the pistons in the upper and lower piston cylinders respectively push and pull the moving block to move downwards. At this time, the moving block drives the push-pull rod to move downwards, and then the hinge rod pushes the surrounding clamping frames to expand outward. Then, the expanded clamping frames are sleeved on the periphery of the sampling biological body, and then the micro air pump is started to draw air from above and exhaust air from below, so that the clamping frames are closed to each other, so as to control the biological body in the four clamping frames, thereby completing sampling.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological sampling, in particular to an intertidal zone biological sampling method and device. BACKGROUND

[0002] Intertidal zone organisms, also known as tidal zone organisms, are all animals and plants that live in the intertidal zone between the highest high tide line and the lowest low tide line. Due to the influence of alternating air and seawater submersion, and the obvious diurnal, monthly and annual periodic changes, the organisms in this zone have ecological characteristics such as amphibiousness (expressed as eurythermy, euryhalinity, drought tolerance and hypoxia tolerance), rhythmicity (the activity peak of general organisms is consistent with the high tide period), and zonation (zonal distribution caused by different dry and wet conditions adapted by different organisms). There are many organisms in the intertidal zone of the sea, such as snails and shellfish attached to the surface or crevices of reefs, and shrimps, crabs, clams and mollusks in the mud of the beach. In some cases, when conducting ecological, hydrological and environmental studies, the organisms in the intertidal zone need to be sampled and analyzed.

[0003] In the prior art, when sampling organisms in the intertidal zone of the sea, some snails and shellfish are often attached to the reefs. Simple manual picking may cause finger cuts by sharp shells, and traditional clamps may damage the organisms when clamping due to the need to clamp on the body of the organism. In addition, compared with the sampling of organisms in the mud of the beach, the biological samples in the sampling frame need to be transferred to the sieve frame first, and then the sieve frame and the samples are transported to the water source for washing and distinguishing. The operation process is complex and inefficient, and it is difficult to quickly identify the sampling organisms covered by silt and other impurities. Therefore, in order to solve the above problems, it is necessary to invent an intertidal zone biological sampling method and device. SUMMARY

[0004] The purpose of the present application is to solve the above problems, and the present application provides an intertidal zone biological sampling method and device.

[0005] The technical scheme adopted by the present application is as follows: an intertidal zone biological sampling method and device, comprising a power plate, a top frame fixedly installed on the top of the power plate, a main handle fixedly installed on the rear side of the top frame, a moving block slidingly installed in the middle of the power plate, a piston cylinder fixedly installed above and below the moving block in the power plate, a connecting rod fixedly installed on the top of the moving block and the top, and the piston in the piston cylinder is fixedly connected with the connecting rod, a cavity sleeve plate fixedly installed on the lower front side of the power plate, a push-pull rod fixedly connected with the moving block and inserted and installed in the middle of the cavity sleeve plate, a clamping frame hingedly installed on the bottom of the cavity sleeve plate, a hinge rod hingedly installed on the inner side of the clamping frame, and the end of the hinge rod away from the clamping frame is hingedly connected with the push-pull rod.

[0006] The inner side of the clamping frame is provided with nozzles in linear distribution, the top end of the clamping frame is fixedly connected with a hose connected with the nozzles, the top end of the hose is connected with the inner cavity of the cavity sleeve plate, the top of the moving block is fixedly installed with a micro water pump, the drainage end of the micro water pump is fixedly connected with a bellows, the bottom end of the bellows is connected with the inner cavity of the cavity sleeve plate, the water pumping end of the micro water pump is threadedly connected with an external long tube, and the outer end of the external long tube is fixedly connected with a water collecting cylinder.

[0007] In a preferred mode of the application, the right side of the power plate is fixedly installed with an auxiliary handle, and the outer wall of the main handle and the auxiliary handle is sleeved with a rubber anti-skid sleeve.

[0008] In a preferred mode of the application, the middle part of the power plate is provided with a sliding groove matched with the moving block, and the moving block is slidingly installed in the sliding groove.

[0009] In a preferred mode of the application, the middle part of the rear side of the power plate is fixedly installed with a micro air pump, and the two air ends of the micro air pump are respectively connected with the upper and lower piston cylinders through pipelines, and the power plate is provided with a storage battery.

[0010] In a preferred mode of the application, the connection end of the main handle and the top frame is provided above with an operation button for controlling the air pumping and discharging direction of the micro air pump.

[0011] In a preferred mode of the application, the middle part of the cavity sleeve plate is provided with a perforation matched with the push-pull rod, and the bottom end of the push-pull rod is located below the cavity sleeve plate through the perforation.

[0012] In a preferred mode of the application, the number of the clamping frames is four, and the four bottom frame plates are respectively located at the bottom four edge positions of the cavity sleeve plate.

[0013] In a preferred mode of the application, the bottom of the clamping frame is fixedly installed with a bottom frame plate, and a plurality of leakage holes are formed in the bottom frame plate.

[0014] In a preferred mode of the application, the connection end of the external long tube and the micro water pump is provided with a threaded joint, the outer wall of the water pumping end of the micro water pump is provided with a threaded groove matched with the threaded joint, the periphery of the water collecting cylinder is provided with a filter groove, and the filter groove is provided with filter gauze.

[0015] In a preferred mode of the application, the use method of the device is as follows:

[0016] S1: Before the device is used, a sampling range is first demarcated by the sampling frame, then the sampler holds the main handle of the device with the right hand and holds the auxiliary handle of the device with the left hand, and the organism in the sampling range is sampled, and when sampling, the micro air pump is first started to draw air from below and exhaust air from above by pressing the operation button, at this time, the pistons in the piston cylinders above and below the moving block generate positive pressure and negative pressure respectively, so that the pistons in the piston cylinders above and below the moving block push and pull the moving block to move downward, at this time, the moving block drives the push-pull rod to move downward, and then the four surrounding clamping frames are expanded outward by the hinged rod to prepare for organism grabbing.

[0017] S2: Then the expanded clamping frame is sleeved around the sampled organism, and then the micro air pump is started to draw air from above and exhaust air from below, which will make the four surrounding clamping frames close to each other, at this time, the bottom frame plate at the bottom of the clamping frame closes the bottom, so as to surround and control the organism in the inside of the four clamping frames to complete the sampling.

[0018] S3: During the sampling process, when it is necessary to flush and distinguish the organism surrounded and controlled in the clamping frame, a shallow pit can be dug in the nearest water-containing beach in the sampling range and put into the water collecting cylinder, or the water collecting cylinder can be directly placed on the shallow sea shore, at this time, the seawater around the water collecting cylinder will pass through the filtration of the gauze on the filter groove to enter the inside of the water collecting cylinder, then the threaded joint at the end of the external connecting long pipe is threadedly connected to the water pumping end of the micro water pump, and then the micro water pump is started, at this time, the seawater in the water collecting cylinder flows through the corrugated pipe, the cavity sleeve plate and the hose by the pumping of the micro water pump, and is finally sprayed out from the nozzle on the inside of the clamping frame, so as to flush the mud and sundries around the sampled organism, to help the sampler quickly identify the sampled organism.

[0019] In summary, due to the adoption of the above technical scheme, the beneficial effects of the present application are:

[0020] 1. In the present application, through the above design, before the device is used, a sampling range is first demarcated by the sampling frame, then the sampler holds the main handle of the device with the right hand and holds the auxiliary handle of the device with the left hand, and the organism in the sampling range is sampled, and when sampling, the micro air pump is first started to draw air from below and exhaust air from above by pressing the operation button, at this time, the pistons in the piston cylinders above and below the moving block generate positive pressure and negative pressure respectively, so that the pistons in the piston cylinders above and below the moving block push and pull the moving block to move downward, at this time, the moving block drives the push-pull rod to move downward, and then the four surrounding clamping frames are expanded outward by the hinged rod to prepare for organism grabbing, then the expanded clamping frame is sleeved around the sampled organism, and then the micro air pump is started to draw air from above and exhaust air from below, which will make the four surrounding clamping frames close to each other, at this time, the bottom frame plate at the bottom of the clamping frame closes the bottom, so as to surround and control the organism in the inside of the four clamping frames to complete the sampling.

[0021] 2. In the present application, when it is necessary to flush and distinguish the biological organisms fixed in the clamping frame during sampling, a shallow pit can be dug in the nearest water-containing beach near the sampling range, or a water collecting cylinder can be directly placed on the shallow sea near the shore. At this time, the seawater around the water collecting cylinder will pass through the filter groove and come into the water collecting cylinder, then the threaded joint at the end of the external long tube is screwed to the water pumping end of the micro water pump, and then the micro water pump is started. At this time, the seawater in the water collecting cylinder flows through the corrugated pipe, the cavity sleeve plate and the hose by the pumping of the micro water pump, and finally is sprayed out from the nozzle on the inner side of the clamping frame, so as to flush the mud and sundries around the sampled biological organisms, helping the sampler to quickly identify the sampled biological organisms. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 is a structural schematic diagram of the present application;

[0023] Figure 2 is a rear view angle schematic diagram of the related structure on the power plate in the present application;

[0024] Figure 3 is a structural schematic diagram of the clamping frame when it is closed in the present application;

[0025] Figure 4 is a structural schematic diagram of the clamping frame when it is expanded in the present application;

[0026] Figure 5 is a structural schematic diagram of the water collecting cylinder in the present application.

[0027] Markings in the figure: 1-power plate, 2-top frame, 3-main handle, 4-secondary handle, 5-moving block, 6-piston cylinder, 7-connecting rod, 8-micro air pump, 9-operation button, 10-cavity sleeve plate, 11-pull rod, 12-clamping frame, 13-hinged rod, 14-bottom frame plate, 15-leakage hole, 16-nozzle, 17-hose, 18-micro water pump, 19-corrugated pipe, 20-external long tube, 21-threaded joint, 22-water collecting cylinder, 23-filter groove. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below, obviously, the described embodiments are part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0029] The embodiments of the present application will be described below in conjunction with Figures 1-5 A method and device for sampling intertidal zone organisms are described in detail.

[0030] EMBODIMENT

[0031] WITH REFERENCE TO Figure 1 , 2 , 3, 4, an intertidal zone biological sampling method and device, including a power plate 1, the top of the power plate 1 is fixedly installed with a top frame 2, the rear side of the top frame 2 is fixedly installed with a main handle 3, the right side of the power plate 1 is fixedly installed with an auxiliary handle 4, the outer wall of the main handle 3 and the auxiliary handle 4 is sleeved and installed with a rubber anti-skid sleeve, the middle part of the power plate 1 is slidably installed with a moving block 5, the middle part of the power plate 1 is provided with a sliding groove matched with the moving block 5, and the moving block 5 is slidably installed in the sliding groove, the upper and lower sides of the power plate 1 are fixedly installed with a piston cylinder 6, the top of the moving block 5 is fixedly installed with a connecting rod 7, and the connecting rod 7 is fixedly connected with the piston in the piston cylinder 6, the middle part of the rear side of the power plate 1 is fixedly installed with a micro air pump 8, and the two air ends of the micro air pump 8 are respectively communicated with the piston cylinders 6 at the upper and lower positions through pipelines, the power plate 1 is provided with a storage battery, the connecting end of the main handle 3 and the top frame 2 is provided above with an operation button 9 for controlling the air suction and exhaust direction of the micro air pump 8, the front lower side of the power plate 1 is fixedly installed with a cavity sleeve plate 10, the middle part of the cavity sleeve plate 10 is insertedly installed with a push-pull rod 11 fixedly connected with the moving block 5 at the top end, the middle part of the cavity sleeve plate 10 is provided with a perforation matched with the push-pull rod 11, and the bottom end of the push-pull rod 11 is located below the cavity sleeve plate 10 through the perforation, the bottom of the cavity sleeve plate 10 is hingedly installed with a clamping frame 12, the number of the clamping frame 12 is four, and the four bottom frame plates 14 are respectively located at the bottom four side positions of the cavity sleeve plate 10, the inner side of the clamping frame 12 is hingedly installed with a hinge rod 13, and the end of the hinge rod 13 away from the clamping frame 12 is hingedly connected with the push-pull rod 11, the bottom of the clamping frame 12 is fixedly installed with a bottom frame plate 14, and a plurality of leakage holes 15 are formed in the bottom frame plate 14.

[0032] Through the above design, before using the device, a sampling range is first delimited by the sampling frame, then the sampler holds the main handle 3 of the device with the right hand and holds the auxiliary handle 4 of the device with the left hand to sample the organisms in the sampling range, when sampling, the micro air pump 8 is first actuated to suck air from below and exhaust air from above by pressing the operation button 9, at this time, the piston cylinders 6 above and below the moving block 5 respectively generate positive pressure and negative pressure, so that the pistons in the piston cylinders 6 above and below push and pull the moving block 5 to move downward, at this time, the moving block 5 drives the push-pull rod 11 to move downward, and then the hinge rod 13 pushes the clamping frames 12 around to expand outward, then the expanded clamping frames 12 are sleeved on the outer periphery of the sampled organisms, then the micro air pump 8 is started to suck air from above and exhaust air from below, and the same principle will make the clamping frames 12 around close to each other, at this time, the bottom frame plate 14 at the bottom of the clamping frame 12 closes the bottom, so as to enclose the organisms in the four clamping frames 12, to complete the sampling.

[0033] WITH REFERENCE TO Figure 1 ,2 , 3, 4, 5, the inner side of the clamping frame 12 is provided with a linearly distributed nozzle 16, the top end of the clamping frame 12 is fixedly connected with a hose 17 communicated with the nozzle 16, the top end of the hose 17 is communicated with the inner cavity of the cavity sleeve plate 10, the top of the moving block 5 is fixedly installed with a micro water pump 18, the drainage end of the micro water pump 18 is fixedly connected with a corrugated pipe 19, the bottom end of the corrugated pipe 19 is communicated with the inner cavity of the cavity sleeve plate 10, the water suction end of the micro water pump 18 is threadedly connected with an external long pipe 20, the outer end of the external long pipe 20 is fixedly connected with a water collecting cylinder 22, the connecting end of the external long pipe 20 and the micro water pump 18 is provided with a threaded joint 21, the outer wall of the water suction end of the micro water pump 18 is provided with a threaded groove matched with the threaded joint 21, the periphery of the water collecting cylinder 22 is provided with a filter groove 23, and filter gauze is arranged on the filter groove 23.

[0034] Through the above design, when it is necessary to flush and distinguish the biological body clamped and fixed in the clamping frame 12 during sampling, a shallow pit can be dug in the nearest water-containing beach near the sampling range and the water collecting cylinder 22 is placed in the pit, or the water collecting cylinder 22 is directly placed on the shallow sea near the shore. At this time, the seawater around the water collecting cylinder 22 will pass through the filter gauze on the filter groove 23 and enter the water collecting cylinder 22. Then, the threaded joint 21 at the end of the external long pipe 20 is threadedly connected with the water suction end of the micro water pump 18, and then the micro water pump 18 is started. At this time, the seawater in the water collecting cylinder 22 is sucked by the micro water pump 18 and flows along the corrugated pipe 19, the cavity sleeve plate 10 and the hose 17, and is finally sprayed out from the nozzle 16 on the inner side of the clamping frame 12. Thus, the silt and sundries around the sampling biological body are flushed, so as to help the sampler to quickly identify the sampling biological body.

[0035] The implementation principle of the intertidal zone biological sampling method and device embodiment of the application is as follows:

[0036] Firstly, before the device is used, a sampling range is first demarcated by the sampling frame, then the sampler holds the main handle 3 of the device with the right hand and holds the auxiliary handle 4 of the device with the left hand, and the biological body in the sampling range is sampled. When sampling, the micro air pump 8 is first started to suck air from below and exhaust air from above, so that the pistons in the upper and lower piston cylinders 6 generate positive pressure and negative pressure respectively, and the pistons in the upper and lower piston cylinders 6 push and pull the moving block 5 to move downward respectively. At this time, the moving block 5 drives the push-pull rod 11 to move downward, and in turn drives the clamping frames 12 around to expand outward, so as to prepare for biological body grabbing.

[0037] Then, the expanded clamping frames 12 are sleeved around the sampling biological body, and then the micro air pump 8 is started to suck air from above and exhaust air from below. According to the same principle, the clamping frames 12 around will be closed to each other, and at this time, the bottom frame plate 14 at the bottom of the clamping frame 12 will close the bottom, so as to enclose the biological body in the four clamping frames 12, so as to complete the sampling.

[0038] Finally, when it is necessary to flush and distinguish the biological object surrounded by the frame 12 during sampling, a shallow pit can be dug in the nearest water-containing beach near the sampling range and the water collecting cylinder 22 is placed in the pit, or the water collecting cylinder 22 is directly placed on the shallow sea near the shore, at this time, the sea water around the water collecting cylinder 22 will be filtered by the gauze on the filter groove 23 to enter the water collecting cylinder 22, then the threaded joint 21 at the end of the long pipe 20 is screwed to the water pumping end of the micro water pump 18, and then the micro water pump 18 is started, at this time, the sea water in the water collecting cylinder 22 will be pumped by the micro water pump 18 to flow along the corrugated pipe 19, the cavity sleeve plate 10 and the hose 17, and finally sprayed out from the nozzle 16 on the inner side of the frame 12, so as to flush the mud and sundries around the sampling biological object, to help the sampler to quickly identify the sampling biological object.

[0039] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting; it is also possible in the practice of the present application that various embodiments can not be presented in this document and that each embodiment presented is intended to include all combinations and permutations of one or more other embodiments presented in this document. It is intended that modifications and variations of the embodiments discussed herein will occur to those skilled in the art; their specific terms are to be considered as not limiting the scope of the embodiments of the present application. It is intended that the present application not be limited to the embodiments described herein, but that it include all embodiments of the present application falling within the scope of the appended claims.

[0040] The above embodiments are only used to illustrate the technical solutions of the present application, rather than limit the present application; even though the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. An intertidal biological sampling device, comprising a power plate (1), characterized in that: A top frame (2) is fixedly installed on the top of the power plate (1), a main handle (3) is fixedly installed on the rear side of the top frame (2), a moving block (5) is slidably installed in the middle of the power plate (1), a piston cylinder (6) is fixedly installed above and below the moving block (5) in the power plate (1), a connecting rod (7) is fixedly installed on the top and bottom of the moving block (5), and the connecting rod (7) is fixedly connected to the piston in the piston cylinder (6), a cavity sleeve plate (10) is fixedly installed on the lower front side of the power plate (1), a push-pull rod (11) with its top end fixedly connected to the moving block (5) is inserted into the middle of the cavity sleeve plate (10), a clamping frame (12) is hingedly installed at the bottom of the cavity sleeve plate (10), a hinge rod (13) is hingedly installed on the inner side of the clamping frame (12), and the end of the hinge rod (13) away from the clamping frame (12) is hinged to the push-pull rod (11); The inner side of the clamping frame (12) is provided with nozzles (16) arranged in a linear distribution. The top of the clamping frame (12) is fixedly connected to a hose (17) that communicates with the nozzles (16). The top of the hose (17) is connected to the inner cavity of the cavity sleeve plate (10). The top of the moving block (5) is fixedly installed with a micro water pump (18). The drain end of the micro water pump (18) is fixedly connected with a corrugated pipe (19). The bottom end of the corrugated pipe (19) is connected to the inner cavity of the cavity sleeve plate (10). The pumping end of the micro water pump (18) is threadedly connected with an external long pipe (20). The outer end of the external long pipe (20) is fixedly connected with a water collecting cylinder (22). The rear middle of the power plate (1) is fixedly installed with a micro air pump (8). The two air ends of the micro air pump (8) are respectively connected to the piston cylinders (6) at the upper and lower positions through pipes. A storage battery is provided inside the power plate (1).

2. The intertidal biological sampling device as described in claim 1, characterized in that: An auxiliary grip (4) is fixedly installed on the right side of the power plate (1), and rubber anti-slip sleeves are fitted on the outer walls of the main grip (3) and the auxiliary grip (4).

3. The intertidal biological sampling device as described in claim 2, characterized in that: The power plate (1) has a groove in the middle that is compatible with the moving block (5), and the moving block (5) is slidably installed in the groove.

4. The intertidal biological sampling device as described in claim 3, characterized in that: An operation button (9) for controlling the air intake and exhaust direction of the micro air pump (8) is provided above the connection end between the main handle (3) and the top frame (2).

5. The intertidal biological sampling device as described in claim 4, characterized in that: The cavity sleeve (10) has a through hole in the middle that is compatible with the push-pull rod (11), and the bottom end of the push-pull rod (11) is located below the cavity sleeve (10) through the through hole.

6. The intertidal biological sampling device as described in claim 5, characterized in that: The number of the clamping frames (12) is four, and the four bottom frame plates (14) are located at the bottom four sides of the cavity sleeve plate (10).

7. The intertidal biological sampling device as described in claim 6, characterized in that: The bottom of the clamping frame (12) is fixedly installed with a bottom frame plate (14), and the bottom frame plate (14) has multiple holes (15).

8. The intertidal biological sampling device as described in claim 7, characterized in that: The connection end of the external long pipe (20) and the micro water pump (18) is provided with a threaded joint (21). The outer wall of the pumping end of the micro water pump (18) is provided with a threaded groove that matches the threaded joint (21). The water collecting cylinder (22) is provided with a filter groove (23) around its perimeter, and a filter gauze is provided on the filter groove (23).

9. The sampling method of the intertidal biological sampling device as described in claim 8, characterized in that: S1: Before using the device, a sampling range is first defined by the sampling frame. Then, the sampling personnel hold the main handle (3) of the device with their right hand and the auxiliary handle (4) of the device with their left hand to sample the organisms within the sampling range. When sampling, the micro air pump (8) is first drawn from the bottom and vented from the top by pressing the operation button (9). At this time, positive pressure and negative pressure are generated in the piston cylinder (6) located above and below the moving block (5), respectively, so that the pistons in the piston cylinder (6) above and below push and pull the moving block (5) downward. At this time, the moving block (5) drives the push-pull rod (11) to move downward, and then pushes the surrounding clamping frame (12) to unfold outward through the hinge rod (13) to prepare for the grasping of organisms. S2: Next, the unfolded clamping frame (12) is fitted around the sampled organism. Then, the micro air pump (8) is started to draw air from the top and exhaust air from the bottom. In the same way, the clamping frames (12) around the perimeter will close each other. At this time, the bottom frame plate (14) at the bottom of the clamping frame (12) will close the bottom, thereby surrounding and controlling the organism inside the four clamping frames (12) to complete the sampling. S3: During the sampling process, when it is necessary to rinse and distinguish the organisms contained within the frame (12), dig a shallow pit in the nearest water-bearing beach within the sampling range, or place the water collection tube (22) directly on the shallow seashore. At this time, the seawater around the water collection tube (22) will be filtered through the gauze on the filter tank (23) and come into the water collection tube (22). Then, connect the threaded connector (21) at the end of the external long pipe (20) to the pumping end of the micro water pump (18), and then start the micro water pump (18). At this time, the seawater in the water collection tube (22) will be drawn by the micro water pump (18) and flow through the corrugated pipe (19), the cavity sleeve plate (10) and the hose (17), and finally spray out from the nozzle (16) on the inner side of the frame (12), thereby rinsing the mud and debris around the sampled organisms to help the samplers quickly identify the sampled organisms.

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