Method for processing astragalus membranaceus with rice-washing water and its application in preparing drugs for treating diarrhea

Abstract

The present invention belongs to the technical field of Chinese herbal medicine processing, and particularly relates to a method for processing Astragalus membranaceus with rice-washing water and its application in the preparation of drugs for treating diarrhea. The processing method is to spray the rice-washing water onto the Astragalus membranaceus slices, moisten them until they are thoroughly penetrated, and then stir-fry them. After stir-frying until dry, Astragalus membranaceus processed with rice-washing water is obtained. The stir-frying temperature is 150-210 °C, the stir-frying time is 6-12 min, and the mass ratio of the rice-washing water to the Astragalus membranaceus slices is 10-30:100. Astragalus membranaceus processed with rice-washing water obtained by the processing method of the present invention has a good therapeutic effect on diarrhea.

Description

Technical Field

[0001] The present invention belongs to the technical field of Chinese herbal medicine processing, and specifically relates to a method for processing Astragalus membranaceus with rice-washing water and its application in the preparation of drugs for treating diarrhea. Background Art

[0002] Astragalus membranaceus, as a perennial herb of the genus Astragalus in the legume family, is widely distributed in Northeast, North and Northwest China and is cultivated in many places across the country. Its root has extremely high medicinal value and is regarded by traditional Chinese medicine as a good medicine for invigorating qi and consolidating the exterior, promoting diuresis, strengthening the heart, reducing blood pressure, and antibacterial, and has significant effects on treating symptoms such as weakness of the body and qi and blood deficiency. However, the original Astragalus membranaceus herb naturally has certain toxicity and adverse reactions, such as possible stomach discomfort, vomiting and other symptoms, which limits its clinical application.

[0003] In order to improve the properties of Astragalus membranaceus, enhance its efficacy, and reduce its potential side effects, various processing methods have been developed in the history of traditional Chinese medicine. These methods include honey-frying, salt-processing, wine-processing, stir-frying without adjuncts, and stir-frying with rice, and each method has its unique advantages and applicable scenarios. Among them, the stir-frying with rice method can gently heat Astragalus membranaceus through the hygroscopicity and heat transfer of rice, which helps to maintain the stability of its properties, and at the same time reduces the toxic components in the medicinal material to a certain extent and reduces the risk of adverse reactions. However, its operation process is relatively cumbersome, and the requirements for controlling the heat and time are relatively high, which limits its practical application. Summary of the Invention

[0004] In view of the deficiencies of the existing technology, the present invention provides a method for processing Astragalus membranaceus with rice-washing water and its application in the preparation of drugs for treating diarrhea. By spraying and moistening Astragalus membranaceus slices with rice-washing water, Astragalus membranaceus fully absorbs the nutritional components of rice-washing water, and through the frying step, the excess water is removed to obtain Astragalus membranaceus with rice-washing water. It not only retains the characteristics of gentle heating and stable properties of the stir-frying with rice method, overcomes the disadvantages of the traditional stir-frying with rice method such as cumbersome operation, high requirements for controlling heat and time, but also further enhances the efficacy of Astragalus membranaceus through the unique role of rice-washing water.

[0005] In order to achieve the above object, the technical solution adopted in the present application is as follows:

[0006] The first object of the present invention is to provide a method for processing Astragalus membranaceus with rice-washing water, comprising the following steps:

[0007] Spray rice-washing water evenly onto Astragalus membranaceus slices, moisten until thoroughly penetrated and then fry, and obtain Astragalus membranaceus with rice-washing water after frying to dryness:

[0008] The frying temperature is 150 - 210 °C, the frying time is 6 - 12 min, and the mass ratio of rice-washing water to Astragalus membranaceus slices is 10 - 30:100.

[0009] Preferably, the moistening time is 1 - 1.5 h and the temperature is 25°C.

[0010] Preferably, the rice-washing water is composed of rice flour and water, and the mass ratio of rice flour to water is 1:50 - 100.

[0011] The second object of the present invention is to provide the application of the above-mentioned rice-washing water Astragalus membranaceus in the preparation of drugs for treating diarrhea, which mainly includes:

[0012] (1) Mix the rice-washing water Astragalus membranaceus with a solvent and decoct, filter and collect the filtrate to obtain the rice-washing water Astragalus membranaceus filtrate;

[0013] (2) Concentrate the rice-washing water Astragalus membranaceus filtrate to obtain the rice-washing water Astragalus membranaceus extract;

[0014] (3) Prepare the rice-washing water Astragalus membranaceus extract by mixing the rice-washing water Astragalus membranaceus extract with physiological saline.

[0015] Preferably, in the step (1), the solvent is ethanol, and the mass ratio of ethanol to the rice-washing water Astragalus membranaceus is 3:1 - 10:1.

[0016] Preferably, filter and collect the filtrate after each decoction, and mix the filtrates to obtain the rice-washing water Astragalus membranaceus filtrate.

[0017] Preferably, in the step (3), the mass ratio of the rice-washing water Astragalus membranaceus extract to physiological saline is 185 g / L.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] 1. The present invention provides a method for processing Astragalus membranaceus with rice-washing water and its application in the preparation of drugs for treating diarrhea. By spraying and moistening the Astragalus membranaceus slices with rice-washing water, the Astragalus membranaceus can fully absorb the nutritional components of the rice-washing water, and the excess water is removed through the frying step to obtain the rice-washing water Astragalus membranaceus. By constructing a model of diarrhea in mice and administering the rice-washing water Astragalus membranaceus in combination with physiological saline, it is found that the rice-washing water Astragalus membranaceus of the present invention has a good therapeutic effect on diarrhea.

[0020] 2. In the prior art, rice and astragalus membranaceus are used for processing and preparation. The rice is placed in a pot and fried until yellow, then the astragalus membranaceus slices are added and fried over a low heat until the surface turns from brownish yellow to yellowish brown. After that, it is taken out and cooled, and the rice and debris are sieved off to obtain the product of stir-fried astragalus membranaceus with rice. However, this method has a rather cumbersome operation process and requires high control over the heat and time. If the heat is too high, it is easy to cause the medicinal materials to burn, losing their medicinal effects and even producing harmful substances; if the heat is too low, the medicinal materials will be unevenly stir-fried, affecting the release of the medicinal effects. In contrast, the present invention uses rice-washing water as the raw material and directly sprays it onto the astragalus membranaceus slices for preparation, simplifying the cumbersome steps in the traditional preparation process and reducing the difficulty of controlling the heat and time. Using rice-washing water to prepare astragalus membranaceus can not only evenly penetrate the nutrients and medicinal properties in the rice into the astragalus membranaceus, but also avoid the problems of the medicinal materials being burned or unevenly stir-fried due to improper heat. In addition, by controlling the mass ratio of the sprayed rice-washing water to the astragalus membranaceus slices, the moistening time, and the frying temperature and time, the preparation process can be more precisely controlled to ensure the full release and retention of the medicinal effects of astragalus membranaceus, improving the preparation efficiency and quality. Description of the Drawings

[0021] Figure 1 It is the response surface diagram of the frying time, temperature, and the amount of rice-washing water on the comprehensive score of astragalus membranaceus with rice-washing water;

[0022] Figure 2 It is the experimental flow chart of mice in each group of Ctrl (normal group), SDD (model group), HQ (raw astragalus membranaceus group), and MHQ (astragalus membranaceus with rice-washing water group);

[0023] Figure 3 It is the diarrhea grade diagram of mice in each group of Ctrl (normal group), SDD (model group), HQ (raw astragalus membranaceus group), and MHQ (astragalus membranaceus with rice-washing water group);

[0024] Figure 4 It is the colon section diagram of mice in each group of Ctrl (normal group), SDD (model group), HQ (raw astragalus membranaceus group), and MHQ (astragalus membranaceus with rice-washing water group);

[0025] Figure 5 It is the diagram of astragalus membranaceus with rice-washing water slices;

[0026] Figure 6 It is the change in the water content of the feces of mice in each group;

[0027] Figure 7 It is the expression levels of the colon inflammatory factors TNF-α, IL-6, and IL-1β in mice in each group. Detailed Embodiments

[0028] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention.

[0029] The following experimental methods and detection methods are all conventional methods unless otherwise specified; the following reagents and raw materials are all commercially available reagents and raw materials unless otherwise specified. The ethanol, methanol, isopropanol, formic acid, and acetonitrile with a volume fraction of 75% used in the present invention are all purchased from Sinopharm Chemical Reagent Co., Ltd.; the reference substances astragaloside IV and calycosin-7-O-β-D-glucoside are all purchased from Chengdu Push Bio-Technology Co., Ltd.; acetonitrile is of chromatographic purity and water is ultrapure water; the astragalus root slices are purchased from Longxi Baohetang Pharmaceutical Co., Ltd., dried, crushed, and passed through a No. 4 sieve, and then placed in a dryer for standby.

[0030] The instruments used in the present invention are an Agilent 1260 high-performance liquid chromatograph; an SK7200HP ultrasonic cleaner (Shanghai Kedao Ultrasonic Instrument Co., Ltd.); an electronic balance (Shanghai Shunyu Hengping Scientific Instrument Co., Ltd.); an electric blast drying oven (Tianjin Test Instrument Co., Ltd.); a horizontal rotator (Taizhou Nuomi Technology Co., Ltd.); an electronically controlled temperature heating mantle (Tianjin Test Instrument Co., Ltd.); an electric thermostatic water bath (Tianjin Test Instrument Co., Ltd.).

[0031] The technical solutions of the present invention will be further explained and illustrated below by examples and comparative examples, as specifically shown below:

[0032] Example 1

[0033] A method for processing astragalus with rice-washing water includes the following steps:

[0034] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water, and stir evenly to form rice-washing water;

[0035] Weigh 100 g of astragalus root slices, take 10 mL of rice-washing water and evenly spray it onto the astragalus root slices. After moistening for 1 h until it penetrates to the core, place it in an electric blast drying oven and stir-fry at 170 °C for 8 min to obtain astragalus processed with rice-washing water.

[0036] Example 2

[0037] A method for processing astragalus with rice-washing water is the same as the preparation steps of Example 1, except that the amount of rice-washing water used is 20 mL, and it includes the following steps:

[0038] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water, and stir evenly to form rice-washing water;

[0039] Weigh 100 g of sliced Astragalus membranaceus, take 20 mL of rice-washing water and evenly spray it onto the sliced Astragalus membranaceus. After moistening for 1 h until it penetrates to the core, place it in an electrothermal forced-air drying oven and stir-fry at 170 °C for 8 min to obtain rice-washing water-treated Astragalus membranaceus.

[0040] Example 3

[0041] A method for processing Astragalus membranaceus with rice-washing water is the same as the preparation steps in Example 1, except that the amount of rice-washing water used is 30 mL, and it includes the following steps:

[0042] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water and stir evenly to form rice-washing water;

[0043] Weigh 100 g of sliced Astragalus membranaceus, take 30 mL of rice-washing water and evenly spray it onto the sliced Astragalus membranaceus. After moistening for 1 h until it penetrates to the core, place it in an electrothermal forced-air drying oven and stir-fry at 170 °C for 8 min to obtain rice-washing water-treated Astragalus membranaceus.

[0044] Example 4

[0045] A method for processing Astragalus membranaceus with rice-washing water is the same as the preparation steps in Example 2, except that the stir-frying time is 6 min, and it includes the following steps:

[0046] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water and stir evenly to form rice-washing water;

[0047] Weigh 100 g of sliced Astragalus membranaceus, take 20 mL of rice-washing water and evenly spray it onto the sliced Astragalus membranaceus. After moistening for 1 h until it penetrates to the core, place it in an electrothermal forced-air drying oven and stir-fry at 170 °C for 6 min to obtain rice-washing water-treated Astragalus membranaceus.

[0048] Example 5

[0049] A method for processing Astragalus membranaceus with rice-washing water is the same as the preparation steps in Example 2, except that the stir-frying time is 10 min, and it includes the following steps:

[0050] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water and stir evenly to form rice-washing water;

[0051] Weigh 100 g of sliced Astragalus membranaceus, take 20 mL of rice-washing water and evenly spray it onto the sliced Astragalus membranaceus. After moistening for 1 h until it penetrates to the core, place it in an electrothermal forced-air drying oven and stir-fry at 170 °C for 10 min to obtain rice-washing water-treated Astragalus membranaceus.

[0052] Example 6

[0053] A method for processing Astragalus membranaceus with rice-washing water is the same as the preparation steps in Example 2, except that the frying time is 12 min, and it includes the following steps:

[0054] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water, and stir evenly to form rice-washing water;

[0055] Weigh 100 g of Astragalus membranaceus slices, take 20 mL of rice-washing water and evenly spray it onto the Astragalus membranaceus slices. After moistening for 1 h until thoroughly penetrated, place it in an electrothermal blast drying oven and fry at 170 °C for 12 min to obtain Astragalus membranaceus processed with rice-washing water.

[0056] Example 7

[0057] A method for processing Astragalus membranaceus with rice-washing water is the same as the preparation steps in Example 2, except that the frying temperature is 150 °C, and it includes the following steps:

[0058] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water, and stir evenly to form rice-washing water;

[0059] Weigh 100 g of Astragalus membranaceus slices, take 20 mL of rice-washing water and evenly spray it onto the Astragalus membranaceus slices. After moistening for 1 h until thoroughly penetrated, place it in an electrothermal blast drying oven and fry at 150 °C for 6 min to obtain Astragalus membranaceus processed with rice-washing water.

[0060] Example 8

[0061] A method for processing Astragalus membranaceus with rice-washing water is the same as the preparation steps in Example 2, except that the frying temperature is 190 °C, and it includes the following steps:

[0062] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water, and stir evenly to form rice-washing water;

[0063] Weigh 100 g of Astragalus membranaceus slices, take 20 mL of rice-washing water and evenly spray it onto the Astragalus membranaceus slices. After moistening for 1 h until thoroughly penetrated, place it in an electrothermal blast drying oven and fry at 190 °C for 6 min to obtain Astragalus membranaceus processed with rice-washing water.

[0064] Example 9

[0065] A method for processing Astragalus membranaceus with rice-washing water is the same as the preparation steps in Example 2, except that the frying temperature is 210 °C, and it includes the following steps:

[0066] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water, and stir evenly to form rice-washing water;

[0067] Weigh 100 g of Astragalus membranaceus pieces, take 20 mL of rice-washing water and evenly spray it onto the Astragalus membranaceus pieces. After moistening for 1 h until it penetrates to the core, place it in an electric blast drying oven and stir-fry at 210 °C for 6 min to obtain rice-washing water-treated Astragalus membranaceus.

[0068] Control Example 1

[0069] It is the same as Example 1, except that the dosage of rice-washing water is 40 mL. When the dosage of rice-washing water is 40 mL, the phenomenon of "water damage" occurs to the Astragalus membranaceus pieces, which affects the quality of the medicinal materials. Therefore, the dosage of rice-washing water of 10 - 30 mL is selected for subsequent research.

[0070] On the basis of single-factor experiments, the dosage of rice-washing water (X1), stir-frying time (X2), and stir-frying temperature (X3) are selected as optimization factors, and the 3-factor 3-level Box-Behnken response surface experimental design is carried out using Design-Expert 13 software. Referring to the provisions of the Pharmacopoeia and the literature method, the comprehensive score value (Y) of four indexes, namely the yield of alcohol-soluble extractives (M1), the content of calycosin-7-O-β-D-glucoside (M2), the content of astragaloside IV (M3), and the appearance score (M4), is used as the response value for experimental design.

[0071] Determination of the yield of alcohol-soluble extractives (M1): It is determined according to the hot extraction method in the method for the determination of alcohol-soluble extractives in Part IV of the Chinese Pharmacopoeia (2020 Edition), and the solvent is ethanol with a volume fraction of 75%.

[0072] Determination of the content of calycosin-7-O-β-D-glucoside (M2): The content of calycosin-7-O-β-D-glucoside is determined by high performance liquid chromatography - variable wavelength detector (HPLC-VWD) method.

[0073] (1) Chromatographic conditions: Octadecylsilane chemically bonded silica gel is used as the filler; acetonitrile - water (40:60) is used as the mobile phase; evaporative light scattering detector is used for detection. The number of theoretical plates calculated by the astragaloside IV peak should be not less than 4000.

[0074] (2) Preparation of reference substance solution: Take an appropriate amount of astragaloside IV reference substance, accurately weigh it, and make a solution containing 2 mg per 1 mL with ethanol with a volume fraction of 75% to obtain the reference substance solution;

[0075] (3) Preparation of test solution: Take 1 g of Astragalus membranaceus piece powder, accurately weigh it, add 50 mL of ethanol solution with a volume fraction of 75% containing 4% concentrated ammonia solution, and heat under reflux for 1 hour. After cooling, make up the lost weight, shake well, filter, and take the continuous filtrate to evaporate to dryness. The residue is dissolved with ethanol with a volume fraction of 75%, transferred to a 5 mL volumetric flask, and added with ethanol with a volume fraction of 75% to the scale, shake well, and filter through a 0.22 μm microporous filter membrane to obtain the test solution;

[0076] (4) Investigation of linear relationship: Pipette 0.1, 0.25, 0.4, 0.5, and 1 mL of the astragaloside IV reference solution into 2-mL volumetric flasks respectively, and dilute to the mark with ethanol with a chromatographic volume fraction of 75%. Inject 10 μL of each reference solution with different mass concentrations under the same chromatographic conditions to determine the peak area. Take the natural logarithm of the reference solutions with different mass concentrations and the peak areas, perform linear regression with the logarithm of different mass concentrations against the logarithm of the peak areas, plot the standard curve, and obtain the linear equation as Y = 1.8091X + 9.4476, R2 = 0.9984, indicating that astragaloside IV has a good linear range within 0.1 - 1 mg / mL.

[0077] Determination of astragaloside IV content (M3): The content of astragaloside IV was determined by high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD).

[0078] (1) Chromatographic conditions: Determined according to the high performance liquid chromatography method (General Principles 0512) in the Chinese Pharmacopoeia (2020 Edition). Use octadecylsilane chemically bonded silica gel as the filler; use acetonitrile as mobile phase A and 0.2% formic acid solution as mobile phase B, and perform gradient elution according to the regulations in Table 1; the detection wavelength is 260 nm. The number of theoretical plates calculated based on the calycosin-7-O-β-D-glucoside peak should be not less than 3000.

[0079] Table 1 Chromatographic condition parameter table

[0080] Time (min) Mobile phase A (%) Mobile phase B (%) 0～20 20→40 80→60 20～30 40→60 60→40

[0081] (2) Preparation of reference solution: Weigh an appropriate amount of calycosin-7-O-β-D-glucoside reference substance accurately, dissolve it ultrasonically with ethanol with a volume fraction of 75% to prepare a reference solution of 1 mg / mL.

[0082] (3) Preparation of test solution

[0083] Weigh 1 g of the powder of astragalus root slices accurately, place it in a round-bottom flask, accurately add 50 mL of ethanol with a volume fraction of 75%, weigh, heat under reflux for 4 hours, cool, weigh again, make up the lost weight with ethanol with a volume fraction of 75%, shake well, filter, accurately measure 25 mL of the subsequent filtrate, recover the solvent to dryness, dissolve the residue with ethanol with a volume fraction of 75%, transfer it to a 5-mL volumetric flask, add ethanol with a volume fraction of 75% to the mark, shake well, filter through a 0.22-μm microporous filter membrane, and take the subsequent filtrate to obtain an ethanol extract with a volume fraction of 75% and a concentration of 0.1 g / mL.

[0084] (4) Investigation of linear relationship

[0085] Precisely pipette 0.05, 0.1, 0.2, 0.3, 0.5, and 0.6 mL of the astragaloside reference stock solution into 2-mL volumetric flasks respectively, and dilute to the mark with ethanol with a chromatographic volume fraction of 75%. Inject 10 μL of each reference solution with different mass concentrations under the chromatographic conditions in the determination of astragaloside content (1) to determine the peak area. Taking the peak area as the ordinate "Y" and the injection volume concentration as the abscissa "X", plot the standard curve, and calculate the regression equation as: Y = 27615X + 80.418, R2 = 0.9991, and the linear range is good at 0.025 - 0.3 mg / mL.

[0086] Appearance score (M4): Taking stir-frying to dryness as the standard, no scorch marks or gelatinization should occur, with a full score of 10 points.

[0087] According to the importance of the measured indicators in the quality control of astragalus cut pieces, use the analytic hierarchy process for weighting, with M1 accounting for 20.517%, M2 accounting for 37.284%, M3 accounting for 37.284%, and M4 accounting for 4.914%, to obtain the Y calculation formula: Y = (M1 - M1min) / (M1max - M1min)×20.517% + (M2 - M2min) / (M2max - M2min)×37.284% + (M3 - M3min) / (M3max - M3min)×37.284% + M1 / 100×4.914%, where M1max, M2max, and M3max are the maximum values of the alcohol-soluble extract yield, calycosin-7-O-β-D-glucoside content, and astragaloside content respectively, and M1min, M2min, and M3min are the minimum values of the alcohol-soluble extract yield, calycosin-7-O-β-D-glucoside content, and astragaloside content respectively, and the total score is designed to be 1.0. The design factors and levels are shown in Table 2.

[0088] Table 2 Analysis table of response surface factors and level results

[0089]

[0090]

[0091] After regression fitting of each factor, a multiple quadratic response surface regression model was established. The regression equation was Y = 68.49 + 0.1189X1 - 9.96X2 + 1.50X3 - 3.94X1X2 - 3.55X1X3 - 9.72X2X3 - 20X1² - 10.69X2² - 11.54X3², and R² = 0.9708. From the results of the variance analysis, it can be seen that this fitting model was significant (P < 0.001), and the lack-of-fit term was 0.4591 (P > 0.05), with no significant difference, indicating that the model had a good fitting degree and there were no obvious lack-of-fit factors. In addition, the P value of the X2 term was < 0.05, indicating that the frying temperature in this model had a significant effect on the comprehensive score of Astragalus membranaceus processed with rice-washing water. From the P values of each first-order term, interaction term, and second-order term, the interaction of X2X3 was significant (P < 0.05), and X1², X2², and X3² all reached a highly significant level (P < 0.01), as shown in Table 3 specifically.

[0092] Table 3 Analysis of variance table for the results of the response surface experiment

[0093]

[0094]

[0095] To further evaluate the influence of the interaction between X1, X2, and X3 on the comprehensive score of Astragalus membranaceus processed with rice-washing water and determine the optimal level range of each factor, corresponding response surface plots were made according to the analysis results. Optimization was carried out according to the simulation equation, and the optimized processing technology obtained was that the amount of rice-washing water was 20 mL, the frying temperature was 176.4 °C, and the frying time was 7 min. The predicted value of Y was 71.806.

[0096] Example 10

[0097] Weigh 1 g of rice flour and add it to a beaker containing 50 g of water, and stir evenly to form rice-washing water;

[0098] Weigh 100 g of Astragalus membranaceus slices, take 20 mL of rice-washing water and evenly spray it onto the Astragalus membranaceus slices. After moistening for 1 h until it penetrates to the core, place it in an electric blast drying oven and fry it at 180 °C for 7 min to obtain Astragalus membranaceus processed with rice-washing water.

[0099] Mix Astragalus membranaceus processed with rice-washing water and ethanol in a mass ratio of 3:1 and reflux for 3 times; filter and collect the filtrate after each reflux, and mix the 3 filtrates to obtain the filtrate of Astragalus membranaceus processed with rice-washing water.

[0100] Concentrate the filtrate of Astragalus membranaceus processed with rice-washing water to obtain the extract of Astragalus membranaceus processed with rice-washing water;

[0101] Prepare the extract of Astragalus membranaceus processed with rice-washing water with a concentration of 185 g / L by mixing the extract of Astragalus membranaceus processed with rice-washing water and physiological saline.

[0102] Weigh 300 mg of calycosin-7-O-β-D-glucoside with an electronic balance. Transfer the weighed calycosin-7-O-β-D-glucoside to a 500 mL volumetric flask. Slowly add 200 mL of ethanol with a volume fraction of 75% to dissolve calycosin-7-O-β-D-glucoside. Place the volumetric flask in an ultrasonic cleaner and perform ultrasonic treatment until it is completely dissolved. Then continue to make up the volume with ethanol with a volume fraction of 75% to prepare a 1 mg / mL model solution.

[0103] Weigh 1 g of the powder of Astragalus membranaceus pieces precisely, place it in a round-bottom flask, precisely add 50 mL of ethanol with a volume fraction of 75%, weigh it, heat under reflux for 4 hours, cool it, weigh it again, make up the lost weight with ethanol with a volume fraction of 75%, shake well, filter, precisely measure 25 mL of the subsequent filtrate, recover the solvent to dryness, dissolve the residue with ethanol with a volume fraction of 75%, transfer it to a 5 mL volumetric flask, add ethanol with a volume fraction of 75% to the scale, shake well, filter through a 0.22 μm microporous filter membrane, take the subsequent filtrate to obtain the extract of raw Astragalus membranaceus, and the concentration of the extract of raw Astragalus membranaceus is 0.1 g / mL.

[0104] Use the rice-washing water extract of Astragalus membranaceus, the model solution and the extract of raw Astragalus membranaceus prepared in Example 10 to treat diarrhea respectively, and conduct research based on this:

[0105] 1. Experimental animals:

[0106] 20 SPF-grade male Kunming mice, with a mouse age of 6 - 8 weeks, a body weight of 18 - 22 g, a breeding environment temperature of 18 - 22 °C, a humidity of 50% - 60%, 12 h light-dark alternating lighting, free diet, and adaptively fed for 7 days.

[0107] 2. Animal model:

[0108] Randomly divide 20 mice into the extract group of raw Astragalus membranaceus (HQ), the rice-washing water extract group of Astragalus membranaceus (MHQ), the model group (SDD) and the control group (Ctrl), with 5 mice in each group. Except for the control group, other groups were induced with a spleen-deficiency diarrhea model by rhubarb. Intragastric administration of 20 mL / kg of 10% senna leaf suspension (w / v) once a day for one week. The first day of intragastric administration is recorded as the first day of the experiment. At 9 pm every night, mice in the HQ group, MHQ group and SDD group were treated with the drug at a dosing dose of 10 g / mL, and the Ctrl group was intragastrically administered with normal saline.

[0109] The 2020 edition of the Chinese Pharmacopoeia records that the clinical prescription amount of Astragalus membranaceus pieces is 9 - 30 g. Set 30 g as the adult drug dosage. For an adult with a standard body weight of 60 kg, the daily dosage of the pieces for an adult is 500 mg / kg. Calculated based on a mouse weighing 20 g, the mouse dosage converted from an adult is: mouse = adult × conversion coefficient = 150 mg / kg × 12.33 = 1849.5 mg / kg.

[0110] For 7 consecutive days of continuous drug administration, the intuitive effects of each drug on diarrhea treatment were investigated using the diarrhea grade score as the apparent index, and the effects of each drug on the colon were investigated using the content of inflammatory factors in intestinal tissues and other as the effect indexes.

[0111] It should be noted that when the present invention involves a numerical range, it should be understood that both endpoints of each numerical range and any value between the two endpoints can be selected. Since the adopted step methods are the same as those in the embodiments, in order to prevent repetition, the present invention describes the preferred embodiments. Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications to these embodiments once they know the basic creative concept. Therefore, the appended claims are intended to be interpreted to include the preferred embodiments and all changes and modifications falling within the scope of the present invention.

[0112] Figure 1 The results showed that with the increase of frying time and frying temperature, the content of calycosin-7-O-β-D-glucoside showed a trend of first increasing and then decreasing. When the frying time was about 7 minutes, the content was the highest, and when the frying temperature was about 180 °C, the content was the highest.

[0113] Figure 2 The results showed that for the procedures and administration doses of the animal experiments, the animals in all groups were modeled and administered by gavage.

[0114] Figure 3 The results showed that compared with the blank group, the diarrhea of the mice in the model group was the most severe, and the diarrhea grade score was relatively high and extremely significantly increased from the 1st day to the 7th day, and the scores were all between 2 and 3 points; compared with the model group, the diarrhea grade scores of the two drug treatment groups gradually decreased from the second day and showed different degrees of decrease; from the 3rd to the 6th day, the diarrhea grade score of the mice in the Astragalus membranaceus group processed with rice-washing water was extremely significantly lower than that of the model group (P < 0.01); among them, the diarrhea grade score of the Astragalus membranaceus group processed with rice-washing water was generally between 1 and 2 points during the experiment, and the effect of treating diarrhea in mice was better than that of the raw Astragalus membranaceus group. Generally speaking, the antidiarrheal effect of the Astragalus membranaceus group processed with rice-washing water was stronger than that of the raw Astragalus membranaceus.

[0115] Figure 4 The results showed that compared with the blank control group, the goblet cells in the colon tissue of the model group were significantly reduced, and the inflammatory cell infiltration and villus shortening were not obvious. Compared with the model group, there was no obvious change in the goblet cells of the raw Astragalus membranaceus group, and the goblet cells of the Astragalus membranaceus group processed with rice-washing water were significantly increased. Astragalus membranaceus processed with rice-washing water can improve the pathological changes of the colon and treat the colon lesions caused by diarrhea.

[0116] Figure 5 The results showed that the surface of Astragalus membranaceus processed with rice-washing water was dark yellow or brownish-yellow, which was darker than the color of Astragalus membranaceus.

[0117] Figure 6The results showed that there was a highly significant difference in fecal moisture content between the model group and the blank control group (P < 0.01). The sharp increase in fecal moisture content in the model group of mice indicated that the mice in the model group had severe diarrhea and the modeling was successful. After drug treatment, the fecal moisture content in the raw Astragalus membranaceus group and the Astragalus membranaceus processed with rice-washing water group decreased to varying degrees, especially in the Astragalus membranaceus processed with rice-washing water group, where the fecal moisture content decreased highly significantly. This indicated that Astragalus membranaceus processed with rice-washing water treated diarrhea by reducing fecal moisture content.

[0118] Figure 7 The results showed that both Astragalus membranaceus processed with rice-washing water and raw Astragalus membranaceus could treat diarrhea by reducing the expression of pro-inflammatory factors IL-6, IL-1β and TNF-α in the colon of mice, and the therapeutic effect of Astragalus membranaceus processed with rice-washing water was stronger than that of raw Astragalus membranaceus.

[0119] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these changes and modifications.

Claims

1. A method for preparing Astragalus membranaceus with rice water, characterized in that: Spraying rice water onto the astragalus slices, steaming until thoroughly moistened, and then frying, and frying until dry to obtain rice water astragalus; The frying temperature is 180°C, the frying time is 7 minutes, and the mass ratio of rice water to astragalus slices is 10-30:100; The rice swill consists of rice flour and water, and the mass ratio of the rice flour to the water is 1:50-100.

2. The method for processing Astragalus with rice water according to claim 1, characterized in that: The moistening time is 1-1.5 hours, and the temperature is 25°C.

3. Rice water astragalus obtained by the method according to any one of claims 1-2.

4. Use of the rice water astragalus root as claimed in claim 3 in preparing a medicine for treating diarrhea.

5. The use of the rice water astragalus root in the preparation of a drug for treating diarrhea according to claim 4, characterized in that: The rice water astragalus and the solvent are mixed and decocted, and the filtrate is collected and concentrated to obtain the rice water astragalus extract.

6. The use of the rice water astragalus root in the preparation of a drug for treating diarrhea according to claim 5, characterized in that: The solvent is ethanol with a volume fraction of 75%, and the mass ratio of ethanol to rice water astragalus is 3:1-10:

1.

7. The use of the rice water astragalus root in the preparation of a drug for treating diarrhea according to claim 5, characterized in that: After each decoction, filter and collect the filtrate, and mix the filtrate to obtain the rice water astragalus filtrate.

8. The use of the rice water astragalus root in the preparation of a drug for treating diarrhea according to claim 5, characterized in that: Physiological saline is added to the rice water astragalus extract to obtain the rice water astragalus extract.

9. The use of the rice water astragalus root in the preparation of a drug for treating diarrhea according to claim 8, characterized in that: The rice water astragalus extract is mixed with normal saline to prepare a rice water astragalus extract with a concentration of 185 g / L.

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