A sterilization method for traditional Chinese medicine raw materials and its product

Abstract

The present invention belongs to the technical field of traditional Chinese medicine sterilization, and particularly relates to a sterilization method for traditional Chinese medicine raw materials and its products, comprising the following steps: (1) Crushing: Crushing the traditional Chinese medicine raw materials and sieving to obtain medicinal powder; (2) Sterilization: Controlling the water content of the sterilization environment to be 4-5%, heating at a rate of 8-12°C / min, and keeping the medicinal powder at 115-125°C for 2-5 minutes; (3) Cooling: Rapidly cooling the sterilized medicinal powder to room temperature to obtain sterile traditional Chinese medicine raw materials. Compared with the prior art, the content of the active ingredient gentiopicroside in the present invention does not significantly decrease while meeting the sterilization requirements, there is no chemical composition change, the sterilization efficiency is high, the energy consumption is low, the effective sterilization time only needs 2-5 minutes, and there is no risk of solvent residue in the present invention, and no secondary drying is required. Compared with the prior art, it is more suitable for industrial application.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine sterilization, and particularly relates to a sterilization method for traditional Chinese medicine raw materials and a product thereof. Background Art

[0002] With the increasing microbial limit requirements for traditional Chinese medicine (TCM) preparations in pharmacopoeias, choosing the appropriate sterilization method for TCM preparations, especially those containing crude drug powders, has become a major issue for TCM manufacturers. Common TCM sterilization methods include dry heat sterilization, moist heat sterilization, cobalt-60 irradiation, and gas sterilization. These sterilization methods utilize appropriate physical or chemical means to kill viable pathogens and microorganisms in the crude drug powder, thereby achieving sterilization, and have been widely used in preparation production. However, these sterilization methods are not suitable for all TCM raw materials. Improper sterilization methods may damage and alter the active ingredients, affecting efficacy and even causing toxicity. Furthermore, traditional sterilization methods suffer from high energy consumption and long timescales, impacting economic efficiency. The drawbacks and problems inherent in these methods significantly impact the quality of TCM and its preparations.

[0003] Gentiana scabra is a commonly used traditional Chinese medicine, known for its ability to clear heat and dampness, and relieve liver and gallbladder fire. Gentiana scabra powder is used as a raw material in several Chinese patent medicines, including Longdan Xiegan Pills, Xiegan Anshen Pills, and Tongqiao Erlong Pills. Gentiana scabra's main active ingredient, gentiopicroside, is sensitive to moisture, heat, and radiation, making it susceptible to degradation during production and processing.

[0004] CN110251651A discloses a traditional Chinese medicine powder preparation for orthopedics and a sterilization method thereof. This method utilizes a comprehensive sterilization process combining 75% ethanol sterilization with intermittent sterilization at room temperature. This invention has low sterilization efficiency and requires alternating sterilization and air-drying processes, which takes a long time to dry, making it unsuitable for large-scale production sterilization. The sterilization process uses a large amount of ethanol, which may leave solvent residues in the product. The workshop also requires explosion-proof facilities, increasing production costs. CN115463233A discloses a method for sterilizing gentian powder, which involves placing the gentian powder in a vacuum tank and sterilizing it by repeatedly vacuuming and filling it with ethanol vapor to kill the microorganisms therein. This sterilization method places high demands on equipment such as the vacuum tank, poses the risk of solvent residues, and is cumbersome to operate, making it unsuitable for large-scale production sterilization.

[0005] Therefore, there is an urgent need to develop a sterilization method that is simple in process, has high sterilization efficiency, and has no effect on the gentiopicroside content. Summary of the Invention

[0006] The present invention aims to solve the problems in the prior art and provides a method for sterilizing Chinese medicine raw materials and a product thereof.

[0007] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] In a first aspect, the present invention provides a method for sterilizing traditional Chinese medicine raw material powder, comprising the following steps:

[0009] (1) Crushing: Crushing the traditional Chinese medicine raw materials and sieving to obtain powder;

[0010] (2) Sterilization: Controlling the water content of the sterilization environment to be 4 - 5%, heating at a rate of 8 - 12 °C / min, and keeping the powder at 115 - 125 °C for 2 - 5 min;

[0011] (3) Cooling: Rapidly cooling the sterilized powder to room temperature to obtain sterile traditional Chinese medicine raw materials.

[0012] In some embodiments, the traditional Chinese medicine raw materials in step (1) are traditional Chinese medicine raw materials containing gentiopicroside; preferably, the traditional Chinese medicine raw materials are gentiana.

[0013] In some embodiments, the sieving in step (1) is through an 80 - 120 mesh sieve; preferably a 100 mesh sieve.

[0014] In some embodiments, the heating rate in step (2) is 10 - 12 °C / min; preferably 11 °C / min.

[0015] In some embodiments, the water content in step (2) is achieved by an electric heating spiral and injecting steam, and the temperature of the steam is 130 - 135 °C.

[0016] In some embodiments, the sterilized powder in step (3) instantaneously enters the cooling area (15 - 20 °C) from the sterilization area (115 - 125 °C), and the rate of change of the powder temperature in different areas is 95 - 110 °C / min. Preferably 100 - 105 °C / min.

[0017] In some embodiments, the room temperature in step (3) is 10 - 30 °C, preferably 15 - 20 °C.

[0018] In a second aspect, the present invention provides a method for preventing the degradation of gentiopicroside during sterilization, comprising the following steps:

[0019] S1. Crushing the traditional Chinese medicine raw materials containing gentiopicroside and sieving to obtain powder;

[0020] S2. Controlling the water content of the sterilization environment to be 4 - 5%, heating at a rate of 8 - 12 °C / min, and keeping the powder at 115 - 125 °C for 2 - 5 min;

[0021] S3. Cooling the sterilized powder to room temperature at a rate of 95 - 110 °C / min.

[0022] In some embodiments, the heating rate in step S2 is 10-12 °C / min; preferably 11 °C / min.

[0023] In some embodiments, the cooling rate in step S3 is 100-105 °C / min.

[0024] In some embodiments, the room temperature in step S3 is 10-30 °C, preferably 15-20 °C.

[0025] In a third aspect, the present invention provides a traditional Chinese medicine raw material sterilized by the above method.

[0026] In a fourth aspect, the present invention provides a traditional Chinese medicine preparation made from the above traditional Chinese medicine raw material powder.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] (1) While meeting the sterilization requirements, the content of the active ingredient gentiopicroside in the present invention does not significantly decrease and there is no chemical composition change.

[0029] (2) The present invention has high sterilization efficiency, low energy consumption, and the effective sterilization time is only 2-5 minutes.

[0030] (3) The present invention has no risk of solvent residue and does not require secondary drying. Compared with the prior art, the present invention is more suitable for industrial application. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a comparison chart of the fingerprint similarities of gentian powder (F23052-001) before and after sterilization in Example 1.

[0032] Figure 2 It is a comparison chart of the fingerprint similarities of gentian powder (F23052-002) before and after sterilization in Example 2.

[0033] Figure 3 It is a comparison chart of the fingerprint similarities of gentian powder (F23052-003) before and after sterilization in Example 3.

[0034] Figure 4 It is a comparison chart of the fingerprint similarities of gentian powder before and after sterilization in Comparative Example 1.

[0035] Figure 5 It is a comparison chart of the fingerprint similarities of gentian powder before and after sterilization in Comparative Example 2.

[0036] Figure 6 It is a comparison chart of the fingerprint similarities of gentian powder before and after sterilization in Comparative Example 3.

[0037] Figure 7 Figure for comparing the similarity of the fingerprint spectra of gentian powder before and after sterilization in Comparative Example 4. Detailed implementation manners

[0038] The present invention will be described below through specific examples to make the technical solutions of the present invention easier to understand and master. However, the present invention is not limited thereto. The described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments.

[0039] In the ranges disclosed herein, the endpoints and any value are not limited to the exact range or value. These ranges or values should be understood to include values close to these ranges or values. For numerical ranges, between the endpoint values of each range, between the endpoint values of each range and a single point value, and between single point values, they can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed herein. Unless the context clearly indicates otherwise, the singular forms "a", "an", and "the" as used herein include both singular and plural referents. Numerical ranges expressed by endpoints include all values and fractions within the corresponding ranges, as well as the expressed endpoints.

[0040] Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of the present invention. The experimental methods described in the following embodiments are all conventional methods unless otherwise specified; the reagents and materials are all commercially available unless otherwise specified.

[0041] Taking specific gentian as an example below, the implementation of the sterilization process of the present invention and the achieved technical effects will be elaborated in detail.

[0042] The gentian slices used in this embodiment are purchased from Anguo Jinglongkang Pharmaceutical Co., Ltd., and the batch number is YB108220801-156.

[0043] In this embodiment, the sterilization equipment used is a continuous electrothermal steam sterilization equipment (manufacturer: safesteril, France, model 450L6), which includes a feeding unit, a sterilization unit and a cooling unit. Among them, the feeding unit is equipped with a feeding hopper and a horizontal feeding screw conveyor, which can stably and continuously feed materials into the sterilization tank. The sterilization unit is configured with a sterilization tank, which conveys the materials to be sterilized through an electrothermal screw, and controls the temperature and humidity of the sterilization environment to sterilize by balancing the electrothermal screw and injecting steam; the processing time of the materials staying in the sterilization tank is controlled and adjusted by the screw and frequency conversion drive; the bidirectional screw and steam injection are directly controlled by the PLC. During the heat treatment of the materials, the temperature and humidity curve of the materials can be calculated in real time and compared with the set target value. If the temperature and humidity curve conforms to the set target value, the bidirectional screw allows the materials to enter the cooling unit. The cooling unit, the sterilized materials enter the cooling unit instantaneously through an automatic switch, and the materials are conveyed by a cooling screw through a cooling tank. The cooling water passes through the interlayer of the cooling tank, and a small amount of filtered dry cold air enters the cooling tank to avoid condensation. Steam will not condense on the product, and no secondary drying is required to ensure that the moisture of the materials is qualified.

[0044] Sterilization method of Gentiana scabra in Example 1

[0045] Crushing: Take 200 kg of Gentiana scabra pieces (YB108220801-156), crush them into fine powder, and pass through a 100-mesh sieve to obtain Gentiana scabra powder (F23052-001);

[0046] Feeding: After adjusting the temperature of the electrothermal screw to 80-85°C, start feeding, and control the feeding screw speed to 9-13 Hz;

[0047] Sterilization: Use the electrothermal screw to raise the temperature, inject filtered steam above the sterilization tank, the steam temperature is 130-135°C, control the moisture content in the sterilization environment to be 4-5%, control the heating rate of Gentiana scabra powder to be 11°C / min, and keep the temperature of Gentiana scabra powder at 120°C for 3 min;

[0048] Cooling: The sterilized Gentiana scabra powder instantaneously enters the cooling area at 18°C from the sterilization area at 120°C. The temperature change rate of Gentiana scabra powder in different areas is 102°C / min. Control the screw speed of the cooling unit to 30 Hz and reduce it to 18°C.

[0049] Sterilization method of Gentiana scabra in Example 2

[0050] Crushing: Take 200 kg of Gentiana scabra pieces (YB108220801-156), crush them into fine powder, and pass through a 100-mesh sieve to obtain Gentiana scabra powder (F23052-002);

[0051] Feeding: After adjusting the temperature of the electric heating spiral to 80 - 85°C, start feeding, and control the feeding spiral speed at 9 - 13 Hz;

[0052] Sterilization: Use the electric heating spiral to raise the temperature, inject filtered steam above the sterilization tank, the steam temperature is 130 - 135°C, control the moisture content in the sterilization environment at 4 - 5%, control the heating rate of gentian powder at 10°C / min, and keep the gentian powder at 115°C for 5 min;

[0053] Cooling: Let the sterilized gentian powder enter the 15°C cooling area instantaneously from the 115°C sterilization area, the temperature change rate of the gentian powder in different areas is 100°C / min, control the spiral speed of the cooling unit at 30 Hz, and reduce it to 15°C.

[0054] Sterilization method of gentian in Example 3

[0055] Crushing: Take 200 kg of gentian slices (YB108220801 - 156), crush them into fine powder, and pass through a 100 - mesh sieve to obtain gentian powder (F23052 - 003);

[0056] Feeding: After adjusting the temperature of the electric heating spiral to 80 - 85°C, start feeding, and control the feeding spiral speed at 9 - .......Hz;

[0057] Sterilization: Use the electric heating spiral to raise the temperature, inject filtered steam above the sterilization tank, the steam temperature is 130 - 135°C, control the moisture content in the sterilization environment at 4 - 5%, control the heating rate of gentian powder at 12°C / min, and keep the gentian powder at 125°C for 2 min;

[0058] Cooling: Let the sterilized gentian powder enter the 20°C cooling area instantaneously from the 125°C sterilization area, the temperature change rate of the gentian powder in different areas is 105°C / min, control the spiral speed of the cooling unit at 30 Hz, and reduce it to 20°C.

[0059] Sterilization method of gentian in Comparative Example 1

[0060] The difference between this comparative example and Example 1 is: different sterilization methods.

[0061] The gentian powder prepared by using the sterilization method of CN115463233A.

[0062] Sterilization method of gentian in Comparative Example 2

[0063] The difference between this comparative example and Example 1 is: higher sterilization heating rate and temperature. The specific method is:

[0064] Crushing: Take 200 kg of gentian slices (YB108220801 - 156), crush them into fine powder, and pass through a 100 - mesh sieve to obtain gentian powder;

[0065] Feeding: After adjusting the temperature of the heating spiral to 80 - 85°C, start feeding, and control the speed of the feeding spiral at 9 - 13 Hz;

[0066] Sterilization: Use the electric heating spiral to raise the temperature, inject filtered steam above the sterilization tank, the steam temperature is 130 - 135°C, control the moisture content in the sterilization tank at 4 - 5%, use the steam and the electric heating spiral to raise the temperature, control the heating rate of gentian powder at 30°C / min, and keep the temperature of the gentian powder at 130°C for 5 min;

[0067] Cooling: The sterilized gentian powder instantly enters the cooling area at 18°C from the sterilization area at 120°C, the temperature change rate of the gentian powder in different areas is 102°C / min, control the speed of the cooling unit spiral at 30 Hz, and reduce it to 18°C, then it is okay.

[0068] Sterilization method of gentian in Comparative Example 3

[0069] The difference between this comparative example and Example 1 is: the sterilization temperature is low, the time is long, and the cooling and temperature reduction are slow.

[0070] The specific method is:

[0071] Crushing: Take 200 kg of gentian slices (YB108220801 - 156), crush them into fine powder, and pass through a 100 - mesh sieve to obtain gentian powder;

[0072] Feeding: After adjusting the temperature of the electric heating spiral to 80 - 85°C, start feeding, and control the speed of the feeding spiral at 9 - 13 Hz;

[0073] Sterilization: Use the electric heating spiral to raise the temperature, inject filtered steam above the sterilization tank, the steam temperature is 130 - 135°C, control the moisture content in the sterilization environment at 4 - 5%, control the heating rate of gentian powder at 11°C / min, and keep the temperature of the gentian powder at 110°C for 10 min;

[0074] Cooling: Take out, and let the sterilized gentian powder cool naturally to 25°C, then it is okay.

[0075] Sterilization method of gentian in Comparative Example 4

[0076] The difference between this comparative example and Example 1 is: the sterilization conditions are different, and wet heat sterilization is adopted. The specific method is:

[0077] Crushing: Take 200 kg of gentian slices (YB108220801 - 156), crush them into fine powder, and pass through a 100 - mesh sieve to obtain gentian powder;

[0078] Sterilization: Pack it into a sterile breathing bag, put it into a steam sterilizer, wet heat at 115°C for 15 min;

[0079] Cooling: Take out the sterile breathing bag and cool it to 18°C, then it's okay.

[0080] I. Detection of the content of gentiopicroside

[0081] Take gentian powder before and after sterilization, and determine the content of gentiopicroside according to the content determination method under the quality standard of gentian in Part I of the Chinese Pharmacopoeia (2020 Edition).

[0082] The results are shown in Table 1 below, where the content change rate is: (content of gentiopicroside before sterilization - content of gentiopicroside after sterilization) * 100 / content of gentiopicroside before sterilization.

[0083] Table 1 Results of the content of gentiopicroside in gentian powder before and after sterilization

[0084]

[0085] Table 2 Results of moisture in gentian powder before and after sterilization

[0086]

[0087]

[0088] Results: In Examples 1 - 3, the sterilization method of the present invention was used to sterilize gentian powder. As can be seen from Table 1, the content of gentiopicroside in the sterilized gentian powder decreased by 0.83% - 4.45% compared with that before sterilization, and the impact on the content of gentiopicroside was relatively small (less than 5%); as can be seen from Table 2, the water content of the medicinal powder after sterilization was lower than that before sterilization. In the sterilization tank, sterilization was carried out by means of an electric heating spiral and injected steam, and no condensation formed on the medicinal powder. The medicinal powder remained dry during the whole sterilization process. Therefore, the impact on the content of gentiopicroside was relatively small.

[0089] Comparing Example 1 and Comparative Example 2, it can be seen that by increasing the heating rate of the medicinal powder and setting the temperature of the medicinal powder to be maintained at 130°C for 5 minutes, the content of gentiopicroside in the sterilized gentian powder decreased by a relatively large margin of 15.19% compared with that before sterilization; it shows that the heating rate and temperature change during sterilization have a greater impact on the content of gentiopicroside.

[0090] Comparing Example 1 and Comparative Example 3, it can be seen that the medicinal powder was kept at 110°C for 10 minutes and the cooling rate of the gentian powder was reduced. The content of gentiopicroside after sterilization decreased by 7.18% compared with that before sterilization. Combining with Table 2, it can be known that the moisture content of the gentian powder after sterilization in Comparative Example 3 increased by 7.06% compared with that before sterilization. Therefore, the decrease in the content of gentiopicroside after sterilization in Comparative Example 3 may be related to the humidity of the medicinal powder and the long heating time.

[0091] Comparing Example 1 and Comparative Example 4, it can be seen that Comparative Example 4 uses moist heat sterilization. As shown in Table 1, the content of gentiopicroside in gentian powder after sterilization decreased to 17.59% compared with that before sterilization; as shown in Table 2, the water content of the powder after sterilization increased by 49.3% compared with that before sterilization. Gentiopicroside is easily degraded under wet and hot conditions, so the content decreased significantly.

[0092] II. Microbiological detection

[0093] Take gentian powder before and after sterilization, and check according to the microbiological limit test methods for non-sterile products in General Principles 1105 and 1106 of the Fourth Part of Chinese Pharmacopoeia 2020 Edition.

[0094] The results are shown in Table 3.

[0095] Table 3 Microbiological limit test results of gentian powder after sterilization

[0096] [[ID=1r7]]

[0097] Results: The sterilization method of gentian powder of the present invention used in Examples 1-3 can effectively kill microorganisms, and the microbiological limit of gentian powder after sterilization meets the requirements of the enterprise's internal control standards.

[0098] Comparing Example 1 and Comparative Example 1, it can be seen that 75% ethanol sterilization is used, sterilization at room temperature and air drying are cycled twice, and the total number of aerobic bacteria in gentian powder after sterilization is 1500 cfu / g (<2000 cfu / g), but the total number of molds and yeasts is 500 cfu / g (>150 cfu / g), indicating that its sterilization ability has decreased and cannot meet the specified sterilization requirements.

[0099] Comparing Example 1 and Comparative Example 3, it can be seen that when the sterilization temperature is reduced, the powder temperature is set at 110 °C for 10 minutes and naturally cooled to 25 °C. The total number of aerobic bacteria in gentian powder after sterilization is 4500 cfu / g (>2000 cfu / g), the total number of molds and yeasts is 250 cfu / g (>150 cfu / g), and the content of bile salt-tolerant Gram-negative bacteria >100 cfu / g, indicating that its sterilization ability has decreased and cannot meet the specified sterilization requirements.

[0100] III. Fingerprint comparison study

[0101] Take gentian powder before and after sterilization, and detect them respectively according to the gentian fingerprint detection method, and determine by high performance liquid chromatography (General Principles 0512 of the Fourth Part of Chinese Pharmacopoeia 2020 Edition).

[0102] Chromatographic conditions and system suitability test

[0103] Using octadecylsilyl silica gel as the filler; using acetonitrile solution as mobile phase A and aqueous solution containing 0.1 wt% phosphoric acid as mobile phase B, performing gradient elution according to the regulations in Table 4 below, with a detection wavelength of 254 nm and a flow rate of 1 mL·min -1 ; The column temperature is 25°C. The number of theoretical plates calculated by gentiopicroside should be not less than 5000.

[0104] Table 4 Gradient System Program

[0105]

[0106] Preparation of Test Solution

[0107] Take an appropriate amount of this product, about 0.5 g, accurately weigh it, place it in a stoppered conical flask, accurately add 50 ml of 70% methanol, weigh it, ultrasonically treat it (power 300 W, frequency 40 kHz) for 30 minutes, let it cool, weigh it again, make up the lost weight with 70% methanol, shake well, filter, and take the subsequent filtrate, that is obtained.

[0108] Determination Method

[0109] Precisely pipette 10 μl of the test solution, inject it into the liquid chromatograph, determine it, record the chromatogram, and that is obtained.

[0110] Fingerprint Evaluation

[0111] For the fingerprints of the test samples before and after sterilization of gentian powder, calculated by the similarity evaluation system for traditional Chinese medicine chromatographic fingerprints, except for the solvent peak and the gentiopicroside chromatographic peak, the similarity between the fingerprint of the test sample after sterilization and that before sterilization should not be less than 0.950.

[0112] The similarity of the fingerprints of gentian before and after sterilization is shown in Table 5 below, and the similarity comparison is shown in Figure 1-7 .

[0113] Table 5 Comparison of the Similarity of the Fingerprints of Gentian before and after Sterilization

[0114] Similarity of fingerprint spectra before and after sterilization Example 1 0.962 Example 2 0.958 Example 3 0.962 Comparative Example 1 0.931 Comparative Example 2 0.863 Comparative Example 3 0.946 Comparative Example 4 0.815

[0115] Result: Comparing the similarity of the fingerprints of gentian powder after sterilization in Examples 1 - 3 with those before sterilization, the similarity is above 0.95, and there is no obvious increase or decrease in the chromatographic peaks in the fingerprints.

[0116] For Comparative Examples 1, 2 and 4, the similarity of the fingerprint chromatograms before and after sterilization is lower than 0.95.

[0117] It shows that: The sterilization process of the gentian powder of the present invention is superior to the sterilization processes of existing gentian products.

[0118] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than limiting the protection scope of the present invention. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present invention shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A method for sterilizing Chinese medicinal raw materials, characterized in that: The steps include: (1) Crushing: crushing the Chinese medicine raw materials, sieving, and obtaining medicinal powder; (2) Sterilization: Control the moisture content of the sterilization environment to 4-5%, increase the temperature at a rate of 8-12°C / min, and keep the powder at 115-125°C for 2-5 minutes; (3) Cooling: The sterilized powder is quickly cooled to room temperature to obtain sterile Chinese medicine raw materials; The Chinese medicinal raw material in step (1) contains gentiopicroside, and the Chinese medicinal raw material is gentian; The water content in step (2) is achieved by an electric heating spiral and injection of steam, the temperature of the steam being 130-135°C; The cooling rate in step (3) is 95-110°C / min. The sterilization equipment is a continuous electric steam sterilization equipment.

2. The sterilization method according to claim 1, characterized in that The heating rate in step (2) is 10-12°C / min.

3. The sterilization method according to claim 2, characterized in that The heating rate is 11°C / min.

4. A method for preventing gentiopicroside from degradation during sterilization, comprising the following steps: S1. Grinding and sieving the Chinese medicinal raw material containing gentiopicroside to obtain a medicinal powder; S2. Control the moisture content of the sterilization environment to 4-5%, increase the temperature at a rate of 8-12°C / min, and keep the powder at 115-125°C for 2-5 minutes; S3. The sterilized powder is cooled to room temperature at a rate of 95-110°C / min; The Chinese medicinal raw material in step S1 is gentian; The water content in step S2 is achieved by an electric heating spiral and steam injection, wherein the temperature of the steam is 130-135°C; The sterilization equipment is a continuous electric steam sterilization equipment.

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