A growth regulator for increasing folic acid content in rice and its application

Abstract

The present invention belongs to the technical field of plant growth regulators, and specifically relates to a growth regulator for increasing the folate content of rice and its application. The folic acid additive in the growth regulator of the present invention can provide exogenous folic acid supplementation for rice, while the antioxidant and acidity regulator can provide a relatively stable environment for folic acid, reducing its oxidation in the environment. The folic acid synthesis substrate is a folic acid synthesis precursor, which synthesizes folic acid through reactions within the plant body, further increasing the folate content of rice. Combined with the effect of the nutritional enhancer, the agent promotes plant cell division and growth and development, thereby improving the metabolic cycle of rice itself, and together with other components, achieves the effect of increasing the folate content of rice.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and particularly relates to a growth regulator for increasing the folic acid content of rice and an application thereof. Background Art

[0002] Folic acid, a coenzyme involved in numerous processes within the human body, plays a vital role in cell division and growth, as well as the synthesis of nucleic acids, amino acids, and proteins. Folic acid deficiency can lead to a variety of diseases, such as megaloblastic anemia and fetal neural tube defects, and increases the risk of cardiovascular disease, colorectal cancer, and other cancers. However, the human body cannot synthesize folic acid on its own; food is the only source of folic acid. Developing high-folic acid products and increasing daily folic acid intake are crucial for maintaining human health.

[0003] Rice, a staple food, has a low folate content, insufficient to meet human needs. Currently, efforts to increase folate levels in rice, primarily through genetic engineering, have made some progress, but adoption remains limited due to various factors. Therefore, developing diverse methods to increase folate content is a future trend. Summary of the Invention

[0004] The present invention aims to provide a growth regulator for increasing the folic acid content of rice and its application. The growth regulator can be directly applied to rice plants, has a simple application method, low safety risks, and can effectively increase the folic acid content in rice.

[0005] The invention provides a growth regulator for increasing the folic acid content of rice. The growth regulator comprises a folic acid additive, an antioxidant and an acidity regulator. The mass ratio of the folic acid additive, the antioxidant and the acidity regulator is 1:20-25:80-100.

[0006] Preferably, the folic acid additive includes one or more of 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, folic acid and tetrahydrofolate.

[0007] Preferably, the antioxidant includes ascorbic acid and / or salicylic acid; and the acidity regulator includes dipotassium hydrogen phosphate and / or disodium hydrogen phosphate.

[0008] Preferably, the folic acid growth regulator further comprises a folic acid synthesis substrate and a nutritional enhancer;

[0009] The mass ratio of the folic acid additive to the folic acid synthesis substrate is 1:6-15;

[0010] The mass ratio of the folic acid additive to the nutritional enhancer is 1:10-70.

[0011] Preferably, the nutritional enhancer includes one or more of brassinolide, phenylalanine, gibberellin, S-abductin and diethylaminoethyl hexanoate.

[0012] Preferably, the folic acid synthesis substrate includes glutamic acid and / or p-aminobenzoic acid.

[0013] The present invention also provides the use of the growth regulator described in the above technical solution in increasing the folic acid content of rice.

[0014] The present invention also provides a method for increasing the folic acid content in rice, comprising dissolving the growth regulator described in the above technical solution in water, and spraying the obtained solution on the rice leaves.

[0015] Preferably, the period for foliar spraying includes the grain filling period, jointing period or heading period.

[0016] Preferably, the dosage of the growth regulator for foliar spraying is 1500 to 3500 g / mu.

[0017] Beneficial effects:

[0018] The present invention provides a growth regulator for increasing the folate content of rice, comprising a folic acid additive, an antioxidant, and an acidity regulator, wherein the mass ratio of the folic acid additive, antioxidant, and acidity regulator is 1:20 to 25:80 to 100. The folic acid additive can be used to exogenously supplement folic acid for rice, while the antioxidant and acidity regulator can provide a relatively stable environment for folic acid, reducing oxidation of folic acid in the environment, maintaining folic acid stability, and maintaining good environmental conditions for rice growth.

[0019] On this basis, the present invention further enhances the growth regulator's effectiveness by adding a folic acid synthesis substrate and a nutritional enhancer to the growth regulator. The folic acid synthesis substrate is a folic acid synthesis precursor that synthesizes folic acid in the plant body, further increasing the folic acid content. Combined with the nutritional enhancer, it promotes plant cell division and development, thereby enhancing the plant's metabolic cycle. Together with the other components, it achieves the effect of increasing the folic acid content in rice.

[0020] Furthermore, the present invention provides an application of the growth regulator described in the above technical solution in increasing the folic acid content of rice, and proposes applying it to rice by foliar spraying. After the growth regulator is absorbed by the leaves, the utilization efficiency of folic acid is improved, and at the same time, nutrients are provided for the growth of rice. The operation is simple and the effect is significant. DETAILED DESCRIPTION

[0021] The present invention provides a growth regulator for increasing the folic acid content of rice. The growth regulator comprises a folic acid additive, an antioxidant and an acidity regulator. The mass ratio of the folic acid additive, the antioxidant and the acidity regulator is preferably 1:20-25:80-100.

[0022] The folic acid additive of the present invention preferably includes one or more of 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, folic acid and tetrahydrofolate, and is an exogenous addition of folic acid to rice. The molecular formulas of 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, folic acid and tetrahydrofolate are C 20 H 25 N7O6、C 20 H 23 N7O7、C 19 H 19 N7O6、C 19 H 23 N7O6. The antioxidant of the present invention preferably includes ascorbic acid and / or salicylic acid. Since folic acid is unstable and easily oxidized, adding an antioxidant on the basis of exogenous folic acid additives can protect folic acid from oxidation. The acidity regulator of the present invention preferably includes dipotassium hydrogen phosphate and / or disodium hydrogen phosphate. Folic acid is unstable in an acidic environment and needs to be in neutral or slightly alkaline conditions to avoid oxidation. Rice is suitable for growth and development under slightly acidic or neutral conditions. Therefore, dipotassium hydrogen phosphate and / or disodium hydrogen phosphate are used to reduce the oxidation of folic acid in the environment, while adjusting the rice growth environment and increasing the folic acid content of rice.

[0023] In the present invention, the growth regulator for increasing the folic acid content of rice preferably also includes a folic acid synthesis substrate and a nutritional enhancer; the mass ratio of the folic acid additive to the folic acid synthesis substrate is preferably 1:6-15; the mass ratio of the folic acid additive to the nutritional enhancer is preferably 1:10-70.

[0024] The folic acid synthesis substrate of the present invention preferably includes glutamic acid and / or p-aminobenzoic acid. Folic acid is composed of pteridine, glutamic acid, and p-aminobenzoic acid in a conjugated form. Therefore, by increasing the two folic acid synthesis substrates of glutamic acid and p-aminobenzoic acid, more folic acid can be synthesized and the folic acid content can be increased.

[0025] The nutritional enhancer of the present invention preferably includes one or more of brassinolide, phenylalanine, gibberellin, S-absorbin, and diethylaminoethyl hexanoate. The selected nutritional enhancer can promote cell division, affect plant growth and development, and work together with other components to increase the folic acid content in rice.

[0026] The preparation method of the growth regulator of the present invention comprises the following steps: dissolving an acidity regulator, an antioxidant, a folic acid additive, a folic acid synthesis substrate and a nutrition enhancer in water in the order and proportion, and spraying the solution on the rice leaves.

[0027] The preferred volume ratio of the growth regulator to water is 1500-3500g:30-70L. The foliar spraying method is preferably applied during the jointing, grain filling, or heading stages, with spraying preferably occurring one hour after sunset on a clear day. The preferred dosage for foliar spraying is 30-70L / mu, based on a solution of the folic acid growth regulator dissolved in water.

[0028] The present invention uses the growth regulator to spray rice exogenously, which is safe and reliable. The exogenously applied folic acid is converted into a form that can be absorbed and utilized by the human body through the plant's own nutrient cycle, and the folic acid content of the rice can be increased by about 20%.

[0029] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0030] The experiments in the following examples were conducted in Shenbei New District, Shenyang City, Liaoning Province, on the Liaohe Plain, with meadow brown soil. Each experiment was repeated three times, with a planting area of ​​0.5 mu per replicate, and the soil fertilization and pest control conditions were the same.

[0031] Example 1

[0032] A growth regulator for increasing the folic acid content of rice and its application:

[0033] 1. Formula of growth regulator: 15g 5-methyltetrahydrofolate calcium, 300g ascorbic acid and 1.2kg dipotassium hydrogen phosphate;

[0034] 2. Application method:

[0035] Experimental varieties: The rice variety is Yanjing 48;

[0036] Dilute the growth regulator formulated in step 1 to 30 L with water and stir evenly to obtain a growth regulator solution;

[0037] Treatment group:

[0038] 1) The growth regulator solution was applied at a rate of 30 L / mu during the jointing stage of rice. Select a sunny day and wait for 1 hour after sunset. The solution was evenly sprayed on the rice leaves. This was recorded as the jointing stage group.

[0039] 2) The growth regulator solution was applied at a rate of 30 L / mu during the rice grain-filling period, on a clear day, and sprayed evenly onto the rice leaves 1 hour after sunset. This was recorded as the grain-filling period group.

[0040] A control group was set up. Except that no growth regulator was sprayed, other planting and management methods of the control group were the same as those of the treatment group.

[0041] After the rice matures, it is harvested on sunny days and placed in a cool and ventilated place to dry. After threshing and hulling, the folic acid content of the rice is determined using high-performance liquid chromatography-tandem mass spectrometry.

[0042] The determination method is as follows: first, the rice is pretreated: after husking, the rice is ground into powder using a grinder for 1.5 minutes, 50 mg is taken, 1 mL of the extract is added to the powder and mixed, and then boiled for 10 minutes and cooled on ice to room temperature; 20 μL of α-amylase is added and mixed, and then incubated at 37°C for 30 minutes; the sample is boiled for 5 minutes to inactivate the α-amylase, cooled on ice, 30 μL of rat serum is added, and incubated at 37°C for 4 hours; the sample is boiled for 5 minutes, cooled on ice for 10 minutes, and centrifuged at 15,000g for 10 minutes at 4°C; finally, the supernatant is clarified and centrifuged at 4°C for 20 minutes, and the filtrate is used for folic acid analysis.

[0043] The filtrate after pretreatment was measured for folic acid content using high performance liquid chromatography-mass spectrometry tandem instrument.

[0044] The results are shown in Table 1. Compared with the rice in the control group, the total folic acid content in the rice group sprayed with folic acid at the jointing stage increased by 16.1%, and the total folic acid content in the rice group sprayed with folic acid at the grain filling stage increased by 20.6%.

[0045] Table 1 Folic acid concentrations in the treatment group and the control group sprayed with 5-methyltetrahydrofolate calcium salt at different times

[0046]

[0047] Example 2

[0048] A growth regulator for increasing the folic acid content of rice and its application:

[0049] 1. Formula of growth regulator: 15g of 5-formyltetrahydrofolate calcium, 300g of ascorbic acid and 1.2kg of dipotassium hydrogen phosphate.

[0050] 2. Application method:

[0051] The experiment was conducted in Xinglongtai Street, Shenbei New District, Shenyang City, Liaoning Province, and the experimental variety was Yanjing 48;

[0052] Under the same planting environment, the growth regulator formulated in Example 2 was sprayed on the leaves according to the application method and application period in Example 1, and a control group without application of the growth regulator was set up. The folic acid content in the rice after application of the two biological regulators was determined in the same manner as in Example 1.

[0053] The results are shown in Table 2: the folic acid content in rice sprayed with 5-formyl at the jointing stage increased by 13.7% compared with the control group, and the folic acid content in rice sprayed with 5-formyl at the grain filling stage increased by 17.7% compared with the control group.

[0054] Table 2 Folic acid concentrations in the treatment group and the control group sprayed with 5-formyltetrahydrofolate calcium salt at different times

[0055]

[0056] Example 3

[0057] A growth regulator for increasing the folic acid content of rice and its application:

[0058] 1. Formula of growth regulator: 15g 5-methyltetrahydrofolate calcium, 300g ascorbic acid, 1.2kg dipotassium hydrogen phosphate, 90g p-aminobenzoic acid, 90g glutamic acid, 300g brassinolide;

[0059] 2. Application method:

[0060] The experiment was conducted in Xinglongtai Street, Shenbei New District, Shenyang City, Liaoning Province, and the experimental variety was Yanjing 48;

[0061] According to the application method in Example 1, the growth regulator in step 1 was sprayed on the leaves during the rice filling period, and a control group without the growth regulator was set up. The folic acid content in the harvested rice was measured according to the method in Example 1.

[0062] The results are shown in Table 3: Compared with the rice in the control group, the total folic acid content in the rice in the treatment group increased by 24.1%.

[0063] Table 3 Folic acid concentrations in the growth regulator spraying treatment group and the control group

[0064]

[0065]

[0066] Example 4

[0067] A growth regulator for increasing the folic acid content of rice and its application:

[0068] 1. Formula of growth regulator: 15g 5-methyltetrahydrofolate calcium, 300g ascorbic acid, 1.2kg dipotassium hydrogen phosphate, 100g phenylalanine, 90g glutamic acid, 300g brassinolide;

[0069] 2. Application method:

[0070] The experiment was conducted in Xinglongtai Street, Shenbei New District, Shenyang City, Liaoning Province, and the experimental variety was Yanjing 48;

[0071] According to the application method in Example 1, the growth regulator in step 1 was sprayed on the leaves during the rice filling period, and a control group without the growth regulator was set up. The folic acid content in the harvested rice was measured according to the method in Example 1.

[0072] The results are shown in Table 4: Compared with the rice in the control group, the total folic acid content in the rice in the treatment group increased by 25.2%.

[0073] Table 4 Folic acid concentrations in the growth regulator spraying treatment group and the control group

[0074]

[0075] It can be concluded from the above examples that the growth regulator of the present invention can effectively increase the folic acid content of rice.

[0076] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A growth regulator for increasing the folic acid content of rice, characterized in that: The invention comprises a folic acid additive, an antioxidant, an acidity regulator, a folic acid synthesis substrate and a nutritional enhancer, wherein the mass ratio of the folic acid additive, the antioxidant and the acidity regulator is 1:20-25:80-100; The antioxidant includes ascorbic acid and / or salicylic acid; the acidity regulator includes dipotassium hydrogen phosphate and / or disodium hydrogen phosphate; The mass ratio of the folic acid additive to the folic acid synthesis substrate is 1:6-15; The mass ratio of the folic acid additive to the nutritional enhancer is 1:10-70; The nutritional enhancer includes one or more of brassinolide, phenylalanine, gibberellin, S-abductin and diethylaminoethyl hexanoate.

2. The growth regulator according to claim 1, characterized in that The folic acid additive includes one or more of 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, folic acid and tetrahydrofolate.

3. The growth regulator according to claim 1, characterized in that The folic acid synthesis substrates include glutamic acid and / or p-aminobenzoic acid.

4. Use of the growth regulator according to any one of claims 1 to 3 in increasing the folic acid content of rice.

5. A method for increasing the folic acid content in rice, characterized in that: The growth regulator according to any one of claims 1 to 3 is dissolved in water, and the obtained solution is sprayed on the rice leaves.

6. The method according to claim 5, characterized in that The spraying period includes the grain filling period, the jointing period or the heading period.

7. The method according to claim 5 or 6, characterized in that The spraying dosage of the growth regulator is 1500-3500 g / mu.

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