Application of protocatechuic acid ethyl ester as feed additive in improving egg laying rate of laying hens

Abstract

The application discloses application of protocatechuic acid ethyl ester as a feed additive to improving egg laying rate of laying hens. The protocatechuic acid ethyl ester is added into feed of the laying hens as the feed additive, so that the anti-aging ability and production performance of the aging laying hens can be improved, and the feed additive is simple to feed, and can be uniformly mixed in the daily ration and fed, and the storage method of the feed additive is simple, and the feed additive can be well stored at normal temperature, and the feed additive does not lose biological activity, has no toxic side effect, has no residual harm, and is safe and effective.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of feed additives, in particular to application of ethyl protocatechuate as a feed additive to improve the egg production rate of laying hens. BACKGROUND

[0002] Laying hens are one of the important poultry in agricultural production, and the egg production and egg quality have an important influence on the economic benefits of farmers. However, laying hens are prone to aging in the long-term feeding process, and with the increase of the age of laying hens, the egg production capacity of aging laying hens is weakened, the egg production rate is gradually reduced, the egg quantity and quality are affected, the appearance of eggs is poor, the eggshell is weak and fragile, the yolk color is lighter, and the taste and nutritional value are not as good as those of eggs produced by young laying hens, resulting in the decrease of the economic benefits of breeders. In today's high-speed social development, the aging laying hen egg production industry also faces the problem of sustainable development. Due to the decrease of the egg production capacity of aging laying hens, breeders need to find a reasonable treatment method, such as selling to slaughterhouses or processing chicken meat. However, the cost of treating aging laying hens is high, and certain resources and technical support are also needed; and the breeders need to update the hen population regularly, which will lead to the waste of chickens and resources. At present, although there are some feed additives on the market for improving the production performance of laying hens, there are few feed additives for anti-aging of laying hens. SUMMARY

[0003] The application aims to provide application of ethyl protocatechuate as a feed additive to improve the egg production rate of laying hens. By adding ethyl protocatechuate as a feed additive to the feed of laying hens, the egg production rate and egg quality of laying hens can be improved.

[0004] The technical scheme provided by the application is as follows: application of ethyl protocatechuate as a feed additive to improve the egg production rate of laying hens.

[0005] The application has the advantages that the ethyl protocatechuate can reduce the oxidative stress of the tissue cells of laying hens, delay the aging of the body, and prolong the production life.

[0006] The application has the advantages that the ethyl protocatechuate can improve the physiological state and reproductive function of laying hens, improve the egg production rate, egg quantity and egg quality of laying hens.

[0007] The application has the advantages that the suitable addition amount of ethyl protocatechuate in the daily ration of laying hens is 200-800 mg / kg.

[0008] The application has the advantages that the optimal addition amount of ethyl protocatechuate in the daily ration of laying hens is 350 mg / kg.

[0009] Compared with the prior art, the application has the advantages that the ethyl protocatechuate as a feed additive in the feed of the laying hens can improve the anti-aging ability and production performance of the aging laying hens, the feeding of the feed additive is simple, the feed additive is uniformly mixed in the daily ration and fed, the storage method of the feed additive is simple, the feed additive can be well stored at room temperature, the feed additive has no toxic side effects and no residual hazards, is safe and effective. DETAILED DESCRIPTION

[0010] The application will be further described in detail below in combination with specific embodiments, and the examples given are only for illustrating the application, rather than limiting the scope of the application. The examples provided below can serve as a guide for further improvement by those skilled in the art, and do not constitute any limitation on the application. In the following examples, the experimental methods are conventional methods, and are performed according to the techniques or conditions described in the literature in the art or according to the product instructions, unless otherwise specified. The materials, reagents and the like used in the following examples can be obtained from commercial channels, unless otherwise specified.

[0011] Example: Application of ethyl protocatechuate as a feed additive.

[0012] The test animals are 480-day-old aging laying hens with close body weight and good health, 60 in total, which are divided into six groups, each group of ten is labeled for cage breeding, free diet and water. A feed additive, i.e. ethyl protocatechuate, also known as 3,4-dihydroxybenzoic acid ethyl ester (EDHB), is added to the feed of the laying hens. The addition amount of the feed additive in the daily ration of the laying hens includes: experiment group 1: 75 mg / kg; experiment group 2: 200 mg / kg, experiment group 3: 350 mg / kg, experiment group 4: 700 mg / kg, experiment group 5: 900 mg / kg. The feed additive is mixed in the feed of the experiment groups every day, and the blank control group is fed with normal feed. The daily egg production of the laying hens is recorded at 6 pm every day for 14 days. Finally, the changes in the egg production and egg quality of the aging laying hens after eating ethyl protocatechuate are investigated through test verification and data analysis. The chicken house is kept clean and ventilated during the test process, with 16h light and suitable temperature. The test results are as follows:

[0013] Table 1. Effect of ethyl protocatechuate on the egg production rate and egg quality of aging laying hens

[0014]

[0015] Table 1 is the change of laying rate and egg quality of laying hens after adding ethyl protocatechuate, and the data ab in the table represents the significant difference analysis of the data. As shown in Table 1, after adding the feed additive ethyl protocatechuate, the aging laying hens in experimental group 3 and experimental group 4 have different degrees of improvement and increase in laying rate, egg white height, and Haugh unit compared with the control group and other experimental groups. According to the curve fitting of the additive amount on the production performance of laying hens, it is found that the suitable additive amount of ethyl protocatechuate in the diet of laying hens is 200-800 mg / kg. The results of experimental group 3 also show that when the additive amount is 350 mg / kg, the aging laying hens have significant improvement in laying rate, egg white height, and Haugh unit compared with other groups, so it is the optimal additive amount among these concentrations. According to the above results, the additive amount can be selected according to the actual situation in application.

[0016] The feed additive ethyl protocatechuate of the present application can play the following roles in the application of anti-aging of laying hens:

[0017] 1. Delaying the aging process of laying hens: It can reduce the oxidative stress of laying hen tissue cells and delay the aging process of the body. This helps to maintain the physiological function and production performance of laying hens, prolongs their production life, and improves the egg production during the entire production cycle.

[0018] 2. Improving laying rate, egg production, and egg quality: It improves the physiological state and reproductive function of laying hens, not only bringing more income and higher economic benefits to breeders, but also providing consumers with healthier and safer food.

[0019] 3. Reducing the environmental pressure caused by replacing laying hens and promoting the sustainable development of the breeding industry, which makes a positive contribution to environmental protection and ecological balance. Frequent replacement of laying hens increases the burden on the environment, including water consumption, feed consumption, and manure disposal. The use of this feed additive can delay the aging of laying hens and is conducive to the benign development of agricultural production.

[0020] In summary, this anti-aging feed additive for laying hens can bring many benefits. This feed additive is simple to feed, and can be uniformly mixed in the diet for feeding. The storage method is simple, and it can be well preserved at room temperature without losing biological activity, without toxic side effects, without residual hazards, and is safe and effective.

[0021] The above is only the preferred specific implementation of the present application, but the protection scope of the present application is not limited to this. Any changes or replacements within the technical scope disclosed by the present application can be easily thought of by those skilled in the art, and should be covered within the protection scope of the present application.

Claims

1. Use of ethyl protocatechuate as a feed additive for increasing the laying rate of laying hens, characterized in that: The adding amount of the protocatechuic acid ethyl ester in the daily ration of the laying hens is 350 mg / kg.

2. Use according to claim 1, characterized in that: The protocatechuic acid ethyl ester can reduce the oxidative stress of the tissues and cells of the laying hens, delay the aging of the body and prolong the production life.

3. Use according to claim 1, characterized in that: The protocatechuic acid ethyl ester can improve the physiological state and the reproductive function of the laying hens, and improve the egg laying rate, the egg laying quantity and the egg laying quality of the laying hens.

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