A bactericidal composition containing benzovinflumizone and fosetyl aluminum and its application

Abstract

The present invention belongs to the field of pesticide sterilization technology and discloses a sterilization composition containing benzovinflumizone and fosetyl-aluminum and its application. The sterilization composition contains the active ingredients benzovinflumizone and fosetyl-aluminum in a mass ratio of 1:35 to 25:1. The sterilization composition of the present invention exhibits excellent synergistic effects within a certain ratio range, can delay and overcome pathogenic bacteria's drug resistance, extend the service life of a single dose, and is safe and highly effective.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide sterilization, and particularly relates to a sterilization composition containing benzovinfluazide and fosetyl aluminum and an application thereof. Background Art

[0002] Fosetyl aluminum, also known as fosetyl aluminum, is an organophosphorus systemic fungicide that can be transported up and down the plant body, exerting both protective and therapeutic effects. Its mechanism of action is to inhibit the synthesis of phosphatidylcholine in pathogens, thereby disrupting the structure of the cytoplasmic membrane.

[0003] Benzovinflumizone, a pyrazoleamide fungicide developed by Syngenta, is a succinate dehydrogenase inhibitor. It targets protein complex II in the mitochondrial electron transport chain of pathogens, affecting their electron transport system, hindering their energy metabolism, inhibiting their growth, and ultimately killing them. Benzovinflumizone has a broad spectrum of activity, is highly effective, and has a long-lasting effect. However, its single site of action leads to a high risk of resistance development. Therefore, combining benzovinflumizone with other fungicides with different mechanisms of action can help manage resistance.

[0004] Chemical control remains the main method for plant disease prevention and control. Due to the long-term, large-scale use of a single agent, pathogens develop significant drug resistance, and the control effect drops sharply. How to effectively control pathogenic bacteria has become one of the bottlenecks for high and stable crop yields. Due to the difficulty in creating new pesticides, the most effective method is to compound and apply fungicides. Reasonable compounding can improve the control effect of a single drug, delay the resistance of pathogenic microorganisms, and also have an inhibitory effect on pathogens that have already developed resistance, reducing the dosage of the drug to reduce costs and toxicity. In order to ensure the sustainable development of agricultural production and extend the service life of a single agent, the inventor compounded benzovinflumazole with triethyl fosetyl aluminum and found that it has a significant synergistic effect on anthrax and rust within a suitable range, can significantly improve the control effect, reduce the number of drug applications and the dosage, slow down the development of pathogen resistance, reduce control costs, and is safe for crops. Summary of the Invention

[0005] To address the problems existing in the prior art, the inventors compounded fosetyl aluminum and benzovindiflupyr in a specific weight ratio to produce a synergistic effect, safely and efficiently enhancing fungicidal activity. This effectively reduces pesticide usage and control costs, while also significantly reducing pesticide residues in agricultural products. Based on this research, the applicants completed the present invention.

[0006] The purpose of the present invention is to provide a fungicide composition with reasonable components, significant synergy, good control effect on target pathogens, low drug cost, and safety for crops.

[0007] In order to achieve the above object, the present invention adopts the following technical solution: a fungicidal composition containing benzovinflumixazole and fosetyl-aluminum, wherein the fungicidal composition contains benzovinflumixazole and fosetyl-aluminum as active ingredients, and the mass ratio of benzovinflumixazole to fosetyl-aluminum is 1:35 to 25:1, or any value within the above numerical range;

[0008] Furthermore, the mass ratio of benzovinflumizone to fosetyl aluminum is 1:35, 1:20, 1:15, 1:10, 1:5, 1:1, 3:1, 5:1, 10:1, 15:1, 25:1, or any value between the above values;

[0009] Furthermore, the mass ratio of benzovinflumazole to fosetyl aluminum is 1:20 to 15:1, or any value within the above numerical range;

[0010] Furthermore, the mass ratio of benzovinflumizone to fosetyl aluminum is 1:20, 1:15, 1:10, 1:5, 1:1, 3:1, 5:1, 10:1, 15:1, or any value between the above values;

[0011] Furthermore, the mass ratio of benzovinflumizone to fosetyl aluminum is 1:20 to 10:1, or any value within the above numerical range;

[0012] Furthermore, the mass ratio of benzovinflumizone to fosetyl aluminum is 1:20, 1:15, 1:10, 1:5, 1:1, 3:1, 5:1, 10:1, or any value between the above values;

[0013] Furthermore, the mass ratio of benzovinflumazole to fosetyl aluminum is 1:10 to 5:1, or any value within the above numerical range;

[0014] Furthermore, the mass ratio of benzovinflumizone to fosetyl aluminum is 1:10, 1:5, 1:1, 3:1, 5:1, or any value between the above values;

[0015] Furthermore, the total weight of the bactericidal composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.1% to 80% of the total weight of the bactericidal composition;

[0016] Furthermore, the fungicidal composition further comprises, in addition to the active ingredient, an agriculturally acceptable adjuvant, wherein the adjuvant is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0017] Furthermore, the wetting agent is selected from a mixture of one or more of sodium lauryl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, saponin powder, silkworm excrement or soapberry powder;

[0018] Furthermore, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ethers, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers;

[0019] Furthermore, the emulsifier is selected from one or more of alkylbenzene sulfonate calcium, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), phenylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, alkyl biphenyl ether disulfonic acid magnesium salt, triethanolamine salt, benzyl dimethylphenol polyoxyethyl ether, alkylphenol formaldehyde resin polyoxyethyl ether, phenethylphenol formaldehyde resin polyoxyethyl ether, phenethylphenol polyoxyethyl polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether or fatty alcohol polyoxyethylene ether;

[0020] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

[0021] Furthermore, the disintegrant is selected from a mixture of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

[0022] Furthermore, the antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea;

[0023] Furthermore, the defoaming agent is selected from silicone oil, silicone compounds, C 10 ~C 20 Saturated fatty acid compounds or C8~C 10 A mixture of one or more fatty alcohol compounds;

[0024] Furthermore, the solvent is selected from a mixture of one or more of N,N-dimethylformamide, cyclohexanone, butyl ether, xylene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octyl pyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methyl pyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate or acetonitrile;

[0025] Furthermore, the stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0026] Furthermore, the penetrant is selected from a mixture of one or more of penetrant JFC, penetrant T, azone or silicone;

[0027] Furthermore, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;

[0028] Furthermore, the filler is selected from a mixture of one or more of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

[0029] The above substances can be obtained commercially;

[0030] Furthermore, the bactericidal composition can be prepared into any dosage form permitted in agriculture, and the dosage form is a solid preparation or a liquid preparation.

[0031] Furthermore, the solid preparation is a direct-use solid preparation, a dispersible solid preparation or a soluble solid preparation;

[0032] Furthermore, the directly-used solid preparation is a powder, granule, pellet, tablet or strip;

[0033] The dispersible solid preparation is a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule or a water-dispersible tablet;

[0034] The soluble solid preparation is a soluble powder, a soluble tablet or a soluble granule;

[0035] Furthermore, the liquid preparation is a solution preparation, a dispersed liquid preparation, an emulsion preparation, a suspension preparation or a multiphase preparation;

[0036] Furthermore, the solution preparation is a soluble solution, a soluble gel, an oil or a film-spreading oil;

[0037] The dispersed liquid preparation is an emulsifiable concentrate, latex, dispersible liquid or ointment;

[0038] The emulsion preparation is a water emulsion, an oil emulsion, a microemulsion or a fat preparation;

[0039] The suspension preparation is a suspension concentrate, a microcapsule suspension concentrate, an oil suspension concentrate or a dispersible oil suspension concentrate;

[0040] The multiphase preparation is a suspoemulsion, a microcapsule suspension-suspension concentrate, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0041] Furthermore, the formulation is in the form of a solid preparation and / or a liquid preparation, the solid preparation is a wettable powder and / or a water-dispersible granule, and the liquid preparation is a suspension.

[0042] Furthermore, the solid preparation includes granules, wettable powders, and water-dispersible granules;

[0043] The liquid preparations include soluble solutions, emulsifiable concentrates, aqueous emulsions, microemulsions, dispersible oil suspensions, suspoemulsions, and microcapsule suspension-suspension concentrates.

[0044] The present invention also discloses the use of the above-mentioned fungicide composition or its preparation for preventing and controlling crop diseases.

[0045] Furthermore, the disease is anthracnose, rust or downy mildew.

[0046] Furthermore, the fungicidal composition and / or its formulation is applied in an effective amount to plants, plant propagation materials and subsequently grown plant organs, cultivation media, materials or spaces.

[0047] The bactericidal composition of the present invention has the following advantages:

[0048] 1) The composition of the present invention has reasonable components and good bactericidal effect. Its activity and bactericidal effect are not the simple addition of the components, but rather a significant synergistic effect. Its prevention effect is better than that of a single dose while reducing the dosage of a single dose;

[0049] 2) The composition of the present invention reduces the dosage of pesticides, reduces the amount of pesticide residues on crops, and alleviates environmental pollution;

[0050] 3) The composition of the present invention is relatively safe for crops. Application of the composition of the present invention can delay and overcome the development of drug resistance of pathogens and extend the service life of a single agent. DETAILED DESCRIPTION

[0051] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0052] Preparation example:

[0053] Preparation Example 1: 32% benzovinflumixil·fosetyl aluminum suspension (1:15)

[0054] Formula: 2% benzovinflumazole, 30% ethyl phosphine aluminum, 2% fatty alcohol polyoxyethylene ether, 5% alkylphenol polyoxyethylene ether phosphate, 3% sodium lignin sulfonate, 5% alkylphenol polyoxyethylene ether, 0.2% xanthan gum, 5% ethylene glycol, 0.2% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance.

[0055] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0056] Preparation Example 2: 16% benzovinflumixil·fosetyl aluminum suspension (3:1)

[0057] Formula: 12% benzovinflumazole, 4% ethyl phosphine aluminum, 2% isomeric alcohol polyoxyethylene ether, 5% styrene phenol polyoxyethylene ether phosphate, 2% naphthalenesulfonate formaldehyde condensate, 0.25% xanthan gum, 5% glycerol, 0.2% carboxyethyl cellulose, 0.2% sodium phenylate, 0.5% silicone oil, and deionized water to make up the balance;

[0058] Preparation method: Same as Preparation Example 1.

[0059] Preparation Example 3: 30% benzovinflumixil·fosetyl aluminum wettable powder (1:5)

[0060] Formula: 5% benzovinflumazole, 25% ethyl aluminum, 8% sodium lignin sulfonate, 5% naphthalene sulfonate formaldehyde condensate, 3% dispersant NNO, 2% sodium lauryl sulfate, 6% white carbon black, and kaolin to make up the balance;

[0061] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring kettle, and pulverized and mixed evenly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0062] Preparation Example 4: 33% benzovinflumixil·fosetyl-aluminum wettable powder (1:10)

[0063] Formula: 3% benzovinflumazole, 30% ethyl aluminum, 12% naphthalenesulfonate formaldehyde condensate, 5% sodium ligninsulfonate, 3% sodium lauryl sulfate, 10% white carbon black, and kaolin to make up the balance;

[0064] Preparation method: Same as Preparation Example 3.

[0065] Preparation Example 5: 45% benzovinflumizone·fosetyl aluminum water dispersible granules (8:1)

[0066] Formula: 40% benzovinflumizole, 5% ethyl aluminum, 15% sodium lignin sulfonate, 5% sodium polycarboxylate, 2% sodium lauryl sulfate, 10% ammonium sulfate, 10% white carbon black, and kaolin to make up the balance;

[0067] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and sieving are carried out to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0068] Preparation Example 6: 30% benzovinflumizone·fosetyl aluminum water dispersible granules (1:5)

[0069] Formula: 5% benzovinflumazole, 25% fosetyl aluminum, 10% naphthalenesulfonate formaldehyde condensate, 8% sodium polycarboxylate, 5% sodium ligninsulfonate, 2% BX powder, 10% white carbon black, and kaolin to make up the balance;

[0070] Preparation method: Same as Preparation Example 5.

[0071] Indoor combined effect test

[0072] Example 1: Indoor joint action test of benzovinflumazole and fosetyl-aluminum complex on wheat rust

[0073] Test basis: The test was conducted in accordance with NY / T 1156.15-2008 Agricultural Industry Standard of the People's Republic of China "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 15: Pot Method for Control of Wheat Leaf Rust".

[0074] Test target: wheat leaf rust fungus (Puccinia recondita f.sp.tritici).

[0075] Preparation of test materials: 20 wheat seeds were sown in each pot. After the wheat seedlings emerged, 10 plants were selected and numbered for later use when they grew to the 2-leaf 1-heart stage.

[0076] Test agents: 95% fosetyl aluminum technical, 96% benzotriazole technical.

[0077] Preparation: Dissolve fosetyl aluminum technical in distilled water. Dissolve benzovindiflupyr technical in acetone and then dilute with 0.1% Tween 80 aqueous solution. Five concentration series were set based on the activity of the agents.

[0078] Chemical treatment: Spray the solution evenly onto the leaves until they are completely wet. Allow the solution to air dry before use. A treatment without chemical was used as a blank control. Each concentration treatment was replicated four times, with one pot per replicate.

[0079] Inoculation and culture: Spray inoculate with spore suspension. After inoculation, incubate the wheat seedlings in a dark environment at around 20°C for at least 12 hours in a moisturized state. Then, incubate at a temperature of 18-22°C with a light intensity ratio of L:D = 12:12h.

[0080] Data investigation: When the incidence rate of the blank control reaches more than 80%, conduct a graded investigation on the incidence of each treatment.

[0081] The grading method is:

[0082] Level 0: No spore pile;

[0083] Level 1: spore accumulation occupies less than 5% of the entire leaf area;

[0084] Level 3: spore piles occupy 5-10% of the entire leaf area;

[0085] Level 5: spore piles occupy 10-25% of the entire leaf area;

[0086] Level 7: Spore piles occupy 25-50% of the entire leaf area;

[0087] Level 9: The spore pile occupies more than 50% of the entire leaf area.

[0088] Data statistics and analysis:

[0089] Based on the data survey, the disease index and prevention and control effect of each treatment were calculated.

[0090] The disease index is calculated according to the following formula:

[0091]

[0092] The control effect is calculated according to the following formula:

[0093]

[0094] Statistical analysis:

[0095] The DPS statistical analysis system was used to determine the toxicity regression line and EC 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient R.

[0096] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0097]

[0098] Where:

[0099] ATI - measured toxicity index of mixture;

[0100] S——EC of standard agent50 , the unit is milligrams per liter (mg / L);

[0101] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0102] TTI=TI A ×P A +TI B ×P B

[0103] Where:

[0104] TTI – Theoretical Toxicity Index of Mixtures;

[0105] TI A ——Agent toxicity index;

[0106] P A ——The percentage of agent A in the mixture, in percentage (%);

[0107] TI B ——Toxicity index of agent B;

[0108] P B ——The percentage of agent B in the mixture, in percentage (%).

[0109]

[0110] Where:

[0111] CTC – Co-toxicity coefficient;

[0112] ATI - measured toxicity index of mixture;

[0113] TTI - Theoretical Toxicity Index of Mixture.

[0114] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0115] The indoor test results are shown in the following table:

[0116] Table 1 Results of indoor combined effect test of benzovinflumazole and phosphine-aluminum complex on wheat rust

[0117] Test drug Regression equation (Y = a + bx) R <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Benzovinflumazole (A) y=4.9997+1.1354x 0.9996 1.0006 - Aluminum phosphite (B) y=3.5297+1.1418x 0.9987 19.3963 - A:B (1:40) y=3.6241+1.3046x 0.9951 11.3408 118.082 A:B (1:35) y=3.7983+1.2093x 0.9986 9.8575 130.250 A:B(1:20) y=3.9981+1.1808x 0.9999 7.0541 146.612 A:B(1:15) y=4.0407+1.2824x 0.9995 5.5983 161.220 A:B(1:10) y=4.2891+1.1652x 0.9990 4.0750 178.182 A:B (1:5) y=4.4476+1.1698x 0.9995 2.9663 160.893 A:B (3:1) y=5.0175+1.1974x 0.9994 0.9669 135.648 A:B (10:1) y=5.0859+1.1644x 0.9957 0.8438 129.771 A:B (25:1) y=5.1003+1.3175x 0.9983 0.8392 123.747 A:B (30:1) y=4.9814+1.3175x 0.9942 1.0340 99.824

[0118] The indoor test results in Table 1 show that the combination of benzovinflumazole and phosphine-aluminum has a good control effect on wheat rust. Benzovinflumazole has a high toxicity to wheat rust fungus. Its EC 50 1.0006 mg.L -1When the mass ratio of benzovinflumazole to fosetyl-aluminum was within the range of 1:35 to 25:1, the co-toxicity coefficient was greater than 120, and the indoor combined effect showed synergistic effect. When the mass ratio of benzovinflumazole to fosetyl-aluminum was within the range of 1:35 to 3:1, the co-toxicity coefficient was greater than 130, and the synergistic effect was obvious. When the mass ratio of benzovinflumazole to fosetyl-aluminum was 1:10, the synergistic effect against wheat rust was the most significant, with a co-toxicity coefficient of 178.182.

[0119] Example 2: Indoor joint effect test of benzovinflumazole and fosetyl-aluminum complex on cucumber anthracnose

[0120] Test basis: The test was conducted in accordance with NY / T 1156.12-2008 “Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 12: Pot Method for Control of Late Blight”.

[0121] Test instruments: moist heat sterilizer, clean workbench, constant temperature light incubator, electric drum windproof drying oven, 1 / 10,000 electronic balance, pipette, alcohol lamp, small beaker, volumetric flask, Erlenmeyer flask, culture dish (Φ9cm), hole puncher, inoculator (Φ0.6cm), ruler, etc.

[0122] Test agents: 95% fosetyl aluminum technical, 96% benzotriazole technical.

[0123] Biological test materials: The test strain is Colletotrichum orbiculare.

[0124] Preparation: Dissolve fosetyl aluminum technical in distilled water. Dissolve benzovindiflupyr technical in acetone and then dilute with 0.1% Tween 80 aqueous solution. Five concentration series were set based on the activity of the agents.

[0125] Test Methods: First, conduct a preliminary screening of the dosage of each test agent. Based on the results, determine the dosage for in-house toxicity testing. Five concentration gradients are set for each agent, with four replicates. During the test, sterile water is used to prepare the required stock solution of each test agent on a sterile clean bench. The corresponding dosage is then prepared as needed. After the PDA culture medium in the Erlenmeyer flask cools to 45°C, the corresponding solution is added using a pipette, shaken, and plated. This creates a series of toxicant-containing plates. PDA plates without the agent are used as controls.

[0126] Use a 6mm diameter borer to punch out bacterial colonies from the edge of the colony and inoculate them onto the center of the prepared plates, one per plate. Place the plates in a 28°C biochemical incubator. After 7 days of incubation, measure the colony diameter using the cross-hatch method. Repeat four times per treatment, and calculate the inhibition rate.

[0127] Data statistics and analysis: Based on the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the target bacteria, the unit is percentage (%), and the calculation results are rounded to two decimal places.

[0128] D=D1-D2

[0129] Where:

[0130] D——colony growth diameter;

[0131] D1 - colony diameter;

[0132] D2——diameter of mushroom cake.

[0133]

[0134] Where:

[0135] I——hyphae growth inhibition rate;

[0136] D0——blank control colony growth diameter;

[0137] D T ——The growth diameter of the colony after treatment with chemicals.

[0138] The data were processed by probability value analysis. The DPS statistical analysis system was used to analyze the toxicity regression line and EC 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient R.

[0139] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0140]

[0141] Where:

[0142] ATI - measured toxicity index of mixture;

[0143] S——EC of standard agent 50 , the unit is milligrams per liter (mg / L);

[0144] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0145] TTI=TI A ×P A +TI B ×P B

[0146] Where:

[0147] TTI – Theoretical Toxicity Index of Mixtures;

[0148] TIA ——Agent toxicity index;

[0149] P A ——The percentage of agent A in the mixture, in percentage (%);

[0150] TI B ——Toxicity index of agent B;

[0151] P B ——The percentage of agent B in the mixture, in percentage (%).

[0152]

[0153] Where:

[0154] CTC – Co-toxicity coefficient;

[0155] ATI - measured toxicity index of mixture;

[0156] TTI - Theoretical Toxicity Index of Mixture.

[0157] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0158] The indoor test results are shown in the following table:

[0159] Table 2 Results of indoor combined effect test of benzovinflumazole and phosphine-aluminum complex on cucumber anthracnose

[0160] Test drug Regression equation (Y = a + bx) R <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Benzovinflumazole (A) y=5.1391+1.1954x 0.9953 0.7649 - Aluminum phosphite (B) y=3.9264+1.1524x 0.9991 8.5429 - A:B(1:20) y=4.1850+1.2062x 0.9980 4.7391 121.454 A:B(1:15) y=4.2217+1.2365x 0.9973 4.2600 122.612 A:B(1:10) y=4.4120+1.2333x 0.9959 2.9975 148.097 A:B (1:5) y=4.6264+1.1455x 0.9954 2.1189 149.614 A:B (1:1) y=4.9856+1.1493x 0.9984 1.0292 136.425 A:B (5:1) y=5.2106+1.2687x 0.9927 0.6823 132.161 A:B (15:1) y=5.2393+1.2690x 0.9914 0.6478 125.201

[0161] As can be seen from Table 2, the combination of benzovinflumizone and fosetyl-aluminum has a good control effect on the growth of cucumber anthracnose. Benzovinflumizone has a high toxicity to cucumber anthracnose. Its EC 50 0.7649 mg.L -1 When the mass ratio of benzovinflumizone to fosetyl-aluminum was within the range of 1:20 to 15:1, the co-toxicity coefficient was greater than 120, and the combined effect showed synergistic effect. When the mass ratio of benzovinflumizone to fosetyl-aluminum was within the range of 1:10 to 5:1, the co-toxicity coefficient was greater than 130, and the synergistic effect was obvious.

[0162] Field efficacy trials

[0163] Example 3: Field efficacy test of benzovinflumizone and fosetyl-aluminum combination against wheat rust

[0164] Test basis: Refer to GB / T 17980.23-2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Fungicides for Control of Cereal Rusts (Leaf Rust, Stripe Rust, and Stem Rust)"

[0165] The test site is a wheat field in Xiaobotou Town, Wudi County, Binzhou City, Shandong Province. The soil fertility and moisture conditions of the test site are uniform, the terrain is relatively flat, and irrigation is convenient.

[0166] Test target: wheat leaf rust;

[0167] Experimental crops and varieties: wheat (Jimai 22);

[0168] Experimental setup: Each plot was arranged in random blocks, with protection rows around the plot. Each treatment was repeated 4 times, with a 20m 2 .

[0169] Application method: Apply the pesticide for the first time during the wheat milky stage, and apply the pesticide for the second time after 7 days. Use conventional spraying method and spray evenly on the front and back of the leaves.

[0170] Survey and statistical methods: The test was conducted 10 days after the last application of the drug. Five random diagonal sampling points were selected in each plot, with 20 plants surveyed at each point. The top three leaves of each plant were surveyed (including the flag leaf if present). The lesions on each leaf were graded based on the percentage of the total leaf area.

[0171] Grading method:

[0172] Level 0: no disease;

[0173] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0174] Level 3: The lesion area accounts for 6% to 25% of the entire leaf area;

[0175] Level 5: The lesion area accounts for 26% to 50% of the entire leaf area;

[0176] Level 7: The lesion area accounts for 51% to 75% of the entire leaf area;

[0177] Level 9: The area of ​​lesions accounts for more than 75% of the entire leaf area.

[0178] Calculation method of drug efficacy:

[0179]

[0180] Where:

[0181] CK0-disease index of blank control area before application;

[0182] CK1-disease index of blank control area after application;

[0183] PT0-disease index before pesticide application in the treatment area;

[0184] PT1-disease index after pesticide application in the treated area.

[0185] According to field investigations, all treatments grew normally at the experimental dosage, and no abnormalities or drug damage were found in any part of the plant, indicating that it is safe for wheat.

[0186] Table 3 Field efficacy test results of benzovinflumazole and phosphine-aluminum complex on wheat rust

[0187]

[0188]

[0189] Field efficacy trials show that the combination of fosetyl-aluminum and mandipropamid has a strong control effect on wheat rust. Ten days after the last application, the control efficacy of the combination treatment group exceeded 82%, significantly superior to the single-dose control group.

[0190] Example 4: Field efficacy test of benzovinflumizone and fosetyl-aluminum combination against cucumber anthracnose

[0191] Test basis: The test refers to GB / T 17980.112-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 112: Fungicides for Control of Cucumber Anthracnose".

[0192] Experimental crop: cucumber (Jinchun No. 2).

[0193] The test site was a vegetable greenhouse in Dongxiaozhuang Town, Zhuolu County, Hebei Province. The previous crop was rapeseed. The management level of the test site was consistent, the water and fertilizer conditions were uniform, and the plant growth was consistent and uniform.

[0194] Experimental design: Each plot has an area of ​​30m 2 The experimental plots were randomly arranged in blocks, and each treatment was repeated 4 times.

[0195] Application time: Use a Gongnong-16 backpack sprayer to evenly spray cucumber plants. The first application begins at the early stage of anthracnose in cucumbers. Seven days after the first application, apply the spray again, for a total of two applications. Prepare the spray solution and spray it evenly and thoroughly on both sides of the cucumber leaves, ensuring that no dripping occurs.

[0196] Experimental investigation: investigate the disease base before applying the pesticide, investigate the disease index before the second application (i.e. 7 days after the first application) and 11 days after the second application, and calculate the prevention and control effect. A total of 3 investigations were conducted.

[0197] Survey method: Sampling method: 5 points per plot, 2 plants per point, and all leaves of the plant are surveyed.

[0198] The classification is carried out according to the following classification method:

[0199] Level 0: no lesions;

[0200] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0201] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0202] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0203] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0204] Level 7: The lesion area accounts for more than 51% of the entire leaf area;

[0205] Calculation method of drug efficacy:

[0206]

[0207] Where:

[0208] CK0-disease index of blank control area before application;

[0209] CK1-disease index of blank control area after application;

[0210] PT0-disease index before pesticide application in the treatment area;

[0211] PT1-disease index after pesticide application in the treated area.

[0212] During the experiment, cucumbers in each treatment plot grew well, and none of the treatment agents caused phytotoxicity to cucumber plants or other non-target organisms at the tested concentrations.

[0213] The results of the field efficacy test are shown in the table below:

[0214] Table 4 Field efficacy test results of benzovinflumazole and fosetyl aluminum complex on cucumber anthracnose

[0215]

[0216] Table 4 shows the field efficacy test results of a combination of benzovinflumizone and fosetyl-aluminum against cucumber anthracnose. The combination demonstrated excellent control efficacy against cucumber anthracnose. Seven days after the first application, the control efficacy of each formulation against cucumber anthracnose was over 80.71%, demonstrating excellent rapid efficacy. Eleven days after the second application, the dosage of 30% benzovinflumizone / fosetyl-aluminum wettable powder (1:5) was 20, 35, and 50 g / hm2. 2 The prevention efficacy against cucumber anthracnose was 83.15%, 86.12% and 87.09% respectively.

[0217] In laboratory toxicity assays and field trials, the fungicidal composition of benzovinflumizone and fosetyl-aluminum used in the present invention demonstrated excellent control efficacy against plant diseases. The resulting fungicidal composition or formulation exhibited significant control efficacy, outperforming the sum of the individual agents in delaying the development of resistance and prolonging the effectiveness. Furthermore, no phytotoxicity was observed in the experiments, demonstrating that the resulting fungicidal composition or formulation, with its enhanced synergistic efficacy, can reduce production and use costs, while maintaining crop safety.

[0218] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A fungicidal composition containing benzovinflumizone and fosetyl-aluminum, characterized in that: The active ingredients of the fungicide composition include benzovinflumixazole and fosetyl aluminum, and the mass ratio of benzovinflumixazole to fosetyl aluminum is 1:35 to 25:

1.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of benzovinflumazole to fosetyl aluminum is 1:20-15:

1.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of benzovinflumazole to fosetyl aluminum is 1:20 to 10:

1.

4. The bactericidal composition according to claim 1, characterized in that The mass ratio of benzovinflumazole to fosetyl aluminum is 1:10 to 5:

1.

5. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100 wt %, and the total weight of the active ingredients accounts for 0.1% to 80% of the total weight of the bactericidal composition.

6. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicide composition also includes agriculturally acceptable adjuvants, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

7. The bactericidal composition according to claim 1, characterized in that The bactericidal composition is prepared into any dosage form permitted in agriculture, and the dosage form is a solid preparation or a liquid preparation.

8. The bactericidal composition according to claim 7, characterized in that The solid preparations include granules, wettable powders, and water-dispersible granules; The liquid preparations include soluble solutions, emulsifiable concentrates, aqueous emulsions, microemulsions, dispersible oil suspensions, suspoemulsions, and microcapsule suspension-suspension concentrates.

9. Use of the fungicidal composition according to any one of claims 1 to 8 for preventing and controlling crop diseases, characterized in that: The disease is anthracnose or rust.

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