Plant dew based on leucothoe and preparation method thereof

Abstract

This invention belongs to the field of plant dew extraction and processing, and relates to a plant dew based on *Chimonanthus praecox* and its preparation method. By weight, the raw material composition of the plant dew based on *Chimonanthus praecox* is: 8-12 parts of aged *Chimonanthus praecox* leaves, 1-3 parts of aged *Silver Needle* peony root, 0.5-1.5 parts of aged honeysuckle, 40-60 parts of deionized water, and 1.8-3.2 parts of anhydrous ethanol; the aged *Chimonanthus praecox* leaves consist of spring leaves and autumn leaves in a mass ratio of 1-3:1-3. This invention uses Soxhlet extraction to extract the effective components of *Chimonanthus praecox*. The preparation method is simple, and by mixing aged *Chimonanthus praecox* leaves, aged *Silver Needle* peony root, and aged honeysuckle as raw materials, the effective antibacterial components are effectively extracted, exhibiting a synergistic antibacterial effect. Compared with plant dew prepared using these three raw materials alone, this method has a better antibacterial effect.

Description

Technical Field

[0001] This invention belongs to the field of plant dew extraction and processing, and relates to a plant dew based on willow-leaf wintersweet and its preparation method. Background Technology

[0002] Plant extracts are products made from plants as raw materials. Through a physicochemical extraction and separation process, specific or concentrated active ingredients are obtained and concentrated without altering their structure. Depending on the extracted plant components, these can include glycosides, acids, polyphenols, polysaccharides, terpenes, flavonoids, alkaloids, etc. Based on their physical properties, they can be classified as plant oils, extracts, powders, crystals, etc. The plant extraction industry has developed rapidly in the last decade, representing the high-value utilization of biomass resources and serving as an interdisciplinary sector encompassing medicine, fine chemicals, and agriculture. The plant extraction industry is similar to traditional Chinese medicine, a cultural treasure left to us by our ancestors. Therefore, developing plant extraction technology to selectively extract specific active ingredients from plants has significant scientific and practical value.

[0003] Willow-leaf wintersweet contains caustic solanine, caustic solanine, and isocaustic solanine, as well as 12 trace elements, 18 amino acids, and various vitamins. These nutrients are easily absorbed and utilized by the human body, and can relieve various ailments. It has high medicinal value and has multiple effects such as clearing heat, antibacterial, preventing colds, relieving summer heat, and suppressing coughs. It is also effective for burns.

[0004] Currently, there are extremely few extracts of *Chimonanthus praecox* on the market, and the product range is limited and largely undifferentiated. In particular, there is almost no market for *Chimonanthus praecox* extract or products based on its extract. In recent years, people's demand for health has been increasing, and the development of bio-based products, especially plant-based products, can effectively address some of the ailments people experience and meet their needs for high-quality health products. Currently, the formula for *Chimonanthus praecox* extract is not yet perfect, and there is a lack of supporting extraction methods.

[0005] In conclusion, it is essential to develop a plant dew based on willow-leaf wintersweet, and how to provide an energy-saving and simple process for preparing plant dew based on willow-leaf wintersweet is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] The technical problem to be solved by this invention is to fill the gap in the market for distilled products of willow-leaf wintersweet, especially plant dew based on willow-leaf wintersweet. This invention provides a plant dew based on willow-leaf wintersweet and its preparation method, and further makes it into a portable spray with anti-inflammatory and bactericidal effects, which can be used for skin discomfort, oral discomfort, and throat discomfort.

[0007] The purpose of this invention is to provide a plant essence based on *Chimonanthus praecox*. The raw materials for this plant essence, by weight, are: 8-12 parts aged *Chimonanthus praecox* leaves, 1-3 parts aged *Silver Needle White Tea*, 0.5-1.5 parts aged honeysuckle, 40-60 parts deionized water, and 1.8-3.2 parts anhydrous ethanol. The aged *Chimonanthus praecox* leaves are composed of spring and autumn leaves, with a mass ratio of 1-3:1-3.

[0008] Preferably, by weight, the following proportions are used: 8, 9, 10, 11, and 12 parts of aged willow-leaf wintersweet leaves; 1, 2, and 3 parts of aged white honeysuckle; 0.5, 1, and 1.5 parts of aged honeysuckle; 40, 50, and 60 parts of deionized water; and 1.8, 2.4, and 3.2 parts of anhydrous ethanol. The ratio of aged willow-leaf wintersweet spring leaves to aged willow-leaf wintersweet autumn leaves is 1:1, 1:2, and 1:3.

[0009] The preparation method of the dried willow leaves and wax plum leaves includes the following steps:

[0010] S1 Withering: Select fresh leaves of *Wax laurel* and separate them into spring and autumn leaves according to the harvesting time. Spread them evenly in containers at a density of 10-20 kg / m³. 2 Then expose it to sunlight to dry for 2-3 days, ensuring that the moisture content of the willow-leaf wintersweet is 60-80 wt%.

[0011] S2 Drying: The spring leaves and autumn leaves of willow-leaf wintersweet are dried using different processes to ensure that the moisture content of the spring and autumn leaves is less than 5 wt%.

[0012] S3 Aging: Remove the stems from the dried spring and autumn leaves of *Wax laurel* and age them separately for 3 to 5 years at a temperature of 20–30°C and a relative humidity of 40%–60% to obtain aged spring and autumn leaves of *Wax laurel*. Mix the obtained aged spring and autumn leaves of *Wax laurel* according to the formula, remove the stems, and obtain the aged leaves of *Wax laurel*.

[0013] Preferably, the temperature is 20, 25 and 30°C, the relative humidity is 40, 50 and 60%, and the aging time is 3, 4 and 5 years.

[0014] The preparation method of the Chenbaihao Silver Needle includes the following steps:

[0015] S4 Withering: Select white silver needle tea leaves and spread them evenly in a container, with a density of 10-20 kg / m³. 2 Then expose it to sunlight to dry for 2 to 3 days, ensuring that the moisture content of the white silver needle is 50 to 70 wt%.

[0016] S5 Drying: Dry the withered Baihao Yinzhen at a temperature of 25-30℃ and a relative humidity of 50%-60% for 12-24 hours, so that the moisture content of the dried Baihao Yinzhen is less than 5wt%.

[0017] Preferably, the temperature is 25 and 30°C, the relative humidity is 50, 55 and 60%, and the drying time is 12, 18 and 24 hours;

[0018] S6 Aging: Remove the stems from the dried Baihao Yinzhen and age it separately for 3 to 5 years at a temperature of 20 to 30°C and a relative humidity of 40% to 60% to obtain the aged Baihao Yinzhen.

[0019] Preferably, the temperatures are 20, 25, and 30°C, the relative humidity is 40, 50, and 60%, and the aging time is 3, 4, and 5 years;

[0020] The preparation method of the aged honeysuckle includes the following steps:

[0021] S7 withering: Select honeysuckle, place it in a high-temperature kettle at a temperature of 70-80℃ and a turning speed of 10-20 rpm for 1-3 hours to ensure that the moisture content of honeysuckle is 50-60wt%.

[0022] Preferably, the temperature is 70, 75 and 80°C, the turning speed is 10, 15 and 20 revolutions per minute, and the processing time is 1, 2 and 3 hours;

[0023] S8 Drying: Dry the withered honeysuckle at a temperature of 25-30℃ and a relative humidity of 40%-60% for 24-36 hours, so that the moisture content of the dried honeysuckle is less than 5wt%.

[0024] S9 Aging: Remove the stems from the dried honeysuckle and age it separately for 3 to 5 years at a temperature of 20 to 30°C and a relative humidity of 40% to 60% to obtain the aged honeysuckle.

[0025] Preferably, the temperature is 20, 25 and 30°C, the relative humidity is 40, 50 and 60%, and the aging time is 3, 4 and 5 years.

[0026] In some embodiments, the drying process of the spring leaves of the willow-leaf wintersweet described in S2 is as follows: the withered spring leaves of the willow-leaf wintersweet are dried in the first stage at a temperature of 20-25°C and a relative humidity of 67%-80% for 8-12 hours; then the temperature is adjusted to 27-28°C and the relative humidity is 50%-60% for the second stage of drying for 8-12 hours, so that the moisture content of the dried willow-leaf wintersweet is less than 5wt%.

[0027] In some embodiments, the drying process of the autumn leaves of the willow-leaf wintersweet described in S2 is as follows: the withered autumn leaves of the willow-leaf wintersweet are dried in the first stage at a temperature of 30-32°C and a relative humidity of 60-75 wt% for 8-12 hours; then the temperature is adjusted to 35-37°C and the relative humidity is adjusted to 50-55% for the second stage of drying for 8-12 hours, so that the moisture content of the dried willow-leaf wintersweet is less than 5 wt%.

[0028] This invention also provides a method for preparing plant dew based on willow-leaf wintersweet, the preparation method comprising the following steps:

[0029] Extraction of S10 plant dew: According to the formula, add aged willow leaves, wintersweet leaves, aged white honeysuckle needles, and aged honeysuckle flowers to a Soxhlet extractor. Add deionized water and anhydrous ethanol to the Soxhlet extractor. Start heating the reaction vessel at the bottom of the Soxhlet extractor and maintain the temperature at 78-90℃. React for 3-9 hours. Observe the color of the liquid refluxed from the Soxhlet extractor into the reaction vessel. When the liquid color is close to colorless, stop heating and collect the extract in the reaction vessel.

[0030] Preferably, the temperature is 78, 84 and 90°C, and the reaction time is 3, 6 and 9 hours.

[0031] S11 Purification of plant dew based on willow-leaf wintersweet: The collected extract was allowed to stand until it separated into layers. The aqueous phase at the bottom was collected to obtain plant dew based on willow-leaf wintersweet.

[0032] S12 Plant Dew Concentration Adjustment Based on Willow-leaf Wintersweet: The obtained plant dew based on willow-leaf wintersweet is adjusted with water according to the required concentration, and then added to the filling machine for filling.

[0033] The present invention has the following advantages:

[0034] (1) All raw materials used in this invention are non-toxic to the human body, ensuring that the products will not cause harm to human health during use.

[0035] (2) The present invention uses Soxhlet extraction to extract the effective components of willow-leaf wintersweet, which has the advantages of high efficiency and low extraction dosage, reducing the energy used in the later concentration of plant dew, and helping to reduce production costs.

[0036] (3) This invention uses aged willow leaves, aged white honeysuckle and aged honeysuckle as raw materials to effectively extract the effective antibacterial components of aged willow leaves, aged white honeysuckle and aged honeysuckle. The antibacterial components of the three raw materials have a synergistic effect, as demonstrated by the Jin Zhengjun Q value method. Compared with the plant dew prepared by using these three raw materials alone, it has a better antibacterial effect. Attached Figure Description

[0037] Figure 1 A schematic diagram of the preparation process of plant dew based on willow-leaf wintersweet; Detailed Implementation

[0038] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings, technical process steps, specific implementation conditions and materials. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0039] Example 1

[0040] Select fresh spring and autumn leaves of the wintersweet, and spread them evenly in a container at a density of 10 kg / m³. 2 The leaves were then exposed to sunlight for two days, and the moisture content of the spring and autumn leaves of *Wax laurel* was measured to be 72.3 wt% and 70.63 wt%, respectively. The withered spring leaves were then dried in the first stage at 20°C and 67% relative humidity for 12 hours; a second stage of drying was then carried out at 27°C and 50% relative humidity for 8 hours, and the moisture content of the spring leaves was measured to be 3.82 wt%. The dried spring leaves were then destemmed and aged at 20°C and 40% relative humidity for three years to obtain aged spring leaves of *Wax laurel*. The withered autumn leaves of *Wax laurel* were dried in the first stage at 30℃ and 60% relative humidity for 8 hours. Then, the temperature was adjusted to 35℃ and 50% relative humidity for the second stage of drying for 8 hours. The moisture content of the dried autumn leaves was measured to be 1.86 wt%. The dried autumn leaves were then destemmed and aged separately at 20℃ and 40% relative humidity for 3 years to obtain aged autumn leaves of *Wax laurel*. One part of the prepared aged spring leaves and one part of the aged autumn leaves were mixed to obtain aged leaves of *Wax laurel* for later use.

[0041] Example 2

[0042] Select fresh spring and autumn leaves of the wintersweet, and spread them evenly in a container at a density of 15 kg / m³. 2The leaves were then exposed to sunlight for 3 days, and the moisture content of the spring and autumn leaves of *Wax laurel* was measured to be 64.6 wt% and 61.28 wt%, respectively. The withered spring leaves were then dried in the first stage at 25°C and 74% relative humidity for 10 hours; a second stage of drying was then carried out at 28°C and 55% relative humidity for 10 hours, and the moisture content of the spring leaves was measured to be 2.46 wt%. The dried spring leaves were then destemmed and stored separately at 25°C and 50% relative humidity for 4 years to obtain aged spring leaves of *Wax laurel*. The withered autumn leaves of *Wax laurel* were dried in the first stage at 31℃ and 68% relative humidity for 10 hours. Then, the temperature was adjusted to 36℃ and 55% relative humidity for a second stage of drying for 10 hours. The moisture content of the dried autumn leaves was measured to be 2.31 wt%. The dried autumn leaves were then destemmed and aged separately at 25℃ and 50% relative humidity for 4 years to obtain aged autumn leaves. One part of the aged spring leaves and two parts of the aged autumn leaves were mixed to obtain aged autumn leaves for later use.

[0043] Example 3

[0044] Select fresh spring and autumn leaves of the wintersweet, and spread them evenly in a container at a density of 20 kg / m³. 2 The leaves were then exposed to sunlight for 3 days, and the moisture content of the spring leaves was tested to be 68.7 wt% and 64.91 wt%. The withered spring leaves were then dried in the first stage at 25℃ and 80% relative humidity for 12 hours; then the temperature was adjusted to 28℃ and 60% relative humidity for the second stage of drying for 12 hours, and the moisture content was tested to be 3.54 wt%. The dried spring leaves were then destemmed and aged at 30℃ and 60% relative humidity for 5 years to obtain aged spring leaves of *Willow Leaf Wintersweet*. The withered autumn leaves of *Wax laurel* were dried in the first stage at 32℃ and 75% relative humidity for 12 hours. Then, the temperature was adjusted to 37℃ and 55% relative humidity for a second stage of drying for 12 hours. The moisture content of the dried autumn leaves was measured to be 2.91 wt%. The dried autumn leaves were then destemmed and aged separately at 30℃ and 60% relative humidity for 5 years to obtain aged autumn leaves. One part of the aged spring leaves and three parts of the aged autumn leaves were mixed to obtain aged autumn leaves for later use.

[0045] Example 4

[0046] Select white silver needle tea leaves and spread them evenly in a container, with a density of 10 kg / m³.2 The withered white tea was then exposed to sunlight for two days, and the moisture content was found to be 64.5 wt%. The dried white tea was then dried at 25°C and 50% relative humidity for 18 hours, and the moisture content was found to be 4.29 wt%. The dried white tea was then destemmed and aged separately at 20°C and 40% relative humidity for three years to obtain the aged white tea.

[0047] Example 5

[0048] Select white silver needle tea leaves and spread them evenly in a container, with a density of 15 kg / m³. 2 Then, it is exposed to sunlight for 3 days to ensure that the moisture content of the Baihao Yinzhen (Silver Needle White Tea) is 60 wt%. The withered Baihao Yinzhen is then dried at 30℃ and 55% relative humidity for 12 hours, and the moisture content of the dried Baihao Yinzhen is tested to be 3.45 wt%. The stems of the dried Baihao Yinzhen are removed, and they are aged separately at 25℃ and 50% relative humidity for 4 years to obtain the aged Baihao Yinzhen.

[0049] Example 6

[0050] Select white silver needle tea leaves and spread them evenly in a container with a density of 20 kg / m³. 2 Then, it is exposed to sunlight for 3 days to ensure that the moisture content of the Baihao Yinzhen (Silver Needle White Tea) is 70 wt%. The withered Baihao Yinzhen is then dried at 30℃ and 60% relative humidity for 24 hours, and the moisture content of the dried Baihao Yinzhen is measured to be 2.46 wt%. The stems of the dried Baihao Yinzhen are removed, and the tea is then aged separately at 30℃ and 60% relative humidity for 5 years to obtain the aged Baihao Yinzhen.

[0051] Example 7

[0052] Selected honeysuckle flowers were placed in a high-temperature autoclave at 70°C and a turning speed of 10 rpm for 3 hours, and the moisture content of the honeysuckle was found to be 57.6 wt%. The withered honeysuckle flowers were then dried at 25°C and 40% relative humidity for 24 hours, and the moisture content of the dried honeysuckle was found to be 3.92 wt%. The dried honeysuckle flowers were then destemmed and aged separately at 20°C and 40% relative humidity for 3 years to obtain the aged honeysuckle.

[0053] Example 8

[0054] Selected honeysuckle flowers were placed in a high-temperature autoclave at 75°C and a turning speed of 15 rpm for 2 hours. The moisture content of the honeysuckle flowers was tested and found to be 59.6 wt%. The withered honeysuckle flowers were then dried at 30°C and 50% relative humidity for 30 hours. The moisture content of the dried honeysuckle flowers was tested and found to be 3.42 wt%. The stems of the dried honeysuckle flowers were removed, and they were aged separately at 25°C and 50% relative humidity for 4 years to obtain the aged honeysuckle flowers.

[0055] Example 9

[0056] Selected honeysuckle flowers were placed in a high-temperature autoclave at 80°C and a turning speed of 20 rpm for 1 hour. The moisture content of the honeysuckle was found to be 57.8 wt%. The withered honeysuckle was then dried at 25°C and 40% relative humidity for 24 hours. The moisture content of the dried honeysuckle was found to be 3.62 wt%. The dried honeysuckle was then destemmed and aged separately at 30°C and 60% relative humidity for 5 years to obtain the aged honeysuckle.

[0057] Example 10

[0058] According to the formula, aged willow-leaf wintersweet leaves, aged white honeysuckle needles, and aged honeysuckle flowers were added to a Soxhlet extractor. Deionized water and anhydrous ethanol were mixed and added to the Soxhlet extractor. The reaction vessel at the bottom of the Soxhlet extractor was heated and maintained at 78°C for 9 hours. The color of the liquid refluxed from the Soxhlet extractor into the reaction vessel was observed. When the liquid color was close to colorless, heating was stopped, and the extract in the reaction vessel was collected. The collected extract was allowed to stand until it separated into layers. The aqueous phase at the bottom was collected to obtain a plant dew based on willow-leaf wintersweet. The concentration of the plant dew was tested to be 24.2 wt%. The obtained plant dew based on willow-leaf wintersweet was diluted with water according to the required concentration and then added to a filling machine for bottling.

[0059] Table 1 Raw material formulation of Example 10

[0060] raw material Number of parts by weight Willow leaves and wintersweet leaves 8 copies Chen Baihao Silver Needle 1 copy Chen Jinyinhua 0.5 copies Deionized water 40 copies Anhydrous ethanol 1.8 copies

[0061] In Table 1, the dried willow leaves of wintersweet were prepared from the autumn leaves of dried willow leaves of wintersweet in Example 1; the dried white silver needles were prepared from Example 4; and the dried honeysuckle was prepared from Example 7.

[0062] Example 11

[0063] According to the formula, aged willow-leaf wintersweet leaves, aged white honeysuckle needles, and aged honeysuckle flowers were added to a Soxhlet extractor. Deionized water and anhydrous ethanol were mixed and added to the Soxhlet extractor. The reaction vessel at the bottom of the Soxhlet extractor was heated and maintained at 84°C for 6 hours. The color of the liquid refluxed from the Soxhlet extractor into the reaction vessel was observed. When the liquid color was close to colorless, heating was stopped, and the extract in the reaction vessel was collected. The collected extract was allowed to stand until it separated into layers. The aqueous phase at the bottom was collected to obtain a plant dew based on willow-leaf wintersweet. The concentration of the plant dew was tested to be 31.4 wt%. The plant dew based on willow-leaf wintersweet was diluted with water according to the required concentration and then added to a filling machine for bottling.

[0064] Table 2 Raw material formula for Example 11

[0065] raw material Number of parts by weight Willow leaves and wintersweet leaves 10 copies Chen Baihao Silver Needle 2 copies Chen Jinyinhua 1 copy Deionized water 50 copies Anhydrous ethanol 2.4 copies

[0066] In Table 2, the dried willow leaves and wax plum leaves were prepared according to Example 2; the dried white silver needles were prepared according to Example 5; and the dried honeysuckle was prepared according to Example 8.

[0067] Example 12

[0068] According to the formula, aged willow-leaf wintersweet leaves, aged white honeysuckle needles, and aged honeysuckle flowers were added to a Soxhlet extractor. Deionized water and anhydrous ethanol were mixed and added to the Soxhlet extractor. The reaction vessel at the bottom of the Soxhlet extractor was heated and maintained at 90°C for 3 hours. The color of the liquid refluxed from the Soxhlet extractor into the reaction vessel was observed. When the liquid color was close to colorless, heating was stopped, and the extract in the reaction vessel was collected. The collected extract was allowed to stand until it separated into layers. The aqueous phase at the bottom was collected to obtain a plant dew based on willow-leaf wintersweet. The concentration of the plant dew was tested to be 27.49 wt%. The obtained plant dew based on willow-leaf wintersweet was diluted with water according to the required concentration and then added to a filling machine for bottling.

[0069] Table 3 Raw material formulation for Example 12

[0070]

[0071]

[0072] In Table 3, the dried willow leaves and wax plum leaves were prepared from Example 3; the dried white silver needles were prepared from Example 6; and the dried honeysuckle was prepared from Example 9.

[0073] Example 13

[0074] The anti-inflammatory function of the dew prepared based on willow-leaf wintersweet prepared in Examples 10-12 was verified by testing the antibacterial rate of the prepared dew against Staphylococcus aureus, Escherichia coli and Candida albicans.

[0075] Test strain: Staphylococcus aureus (ATCC 6538) 3rd generation (provided by Shanghai Beisi Biotechnology Co., Ltd.);

[0076] Escherichia coli (8099) 3rd generation (provided by Shanghai Beisi Biotechnology Co., Ltd.); Candida albicans (ATCC10231) 3rd generation (provided by Shanghai Beisi Biotechnology Co., Ltd.);

[0077] Antibacterial test procedure:

[0078] (1) Melt the agar medium and pour it into a plate.

[0079] (2) Take 0.2 mL of the bacterial solution to be tested and mix it evenly in LB semi-solid medium. Immediately pour it onto agar medium and shake well so that the upper layer of medium covers the plate.

[0080] (3) Using sterile forceps, immerse sterile filter paper in the antibacterial liquid to be tested, and then place it on a sterile agar plate. Record and control the contact time between the filter paper immersed in the antibacterial liquid and the agar plate for 2, 5, 10, and 20 minutes, respectively. Use sterile saline filter paper as a control. The experimental group immersed in the antibacterial liquid to be tested is designated as the experimental group; the experimental group immersed in sterile saline and placed on a sterile agar plate is designated as the positive control group; and the experimental group immersed in sterile saline but not placed on a sterile agar plate is designated as the negative control group.

[0081] (4) Place the plate with the attached filter paper in a 37°C incubator and incubate for 1 day.

[0082] (5) The OD values ​​of each experimental group were tested respectively, and the antibacterial rate was obtained according to formula (1). The test results are shown in Tables 4, 5 and 6 respectively.

[0083]

[0084] Table 4. Antibacterial effect of the plant dew based on *Chimonanthus praecox* prepared in Example 10 on the test bacteria.

[0085]

[0086]

[0087] Note: Negative controls showed no bacterial growth.

[0088] Table 5. Antibacterial effect of the plant dew based on *Chimonanthus praecox* prepared in Example 11 on the test bacteria.

[0089]

[0090] Note: Negative controls showed no bacterial growth.

[0091] Table 6. Antibacterial effect of the plant dew based on *Chimonanthus praecox* prepared in Example 12 on the test bacteria.

[0092]

[0093] Note: Negative controls showed no bacterial growth.

[0094] The results showed that the plant dew based on willow-leaf wintersweet prepared in Examples 10-12 had good antibacterial effects against Staphylococcus aureus, Escherichia coli and Candida albicans, and had good anti-inflammatory effects.

[0095] Example 14

[0096] Meanwhile, to demonstrate the synergistic effect of aged willow-leaf wintersweet leaves, aged white honeysuckle, and aged honeysuckle, this embodiment will also verify the individual efficacy of each ingredient separately. The amount of each ingredient used was 8.5 parts, and the amounts of other raw materials were the same as in Example 10. The same process as in Example 10 was used to prepare plant extracts using only aged willow-leaf wintersweet leaves, aged white honeysuckle, and aged honeysuckle for antibacterial rate experiments.

[0097] Test strain: Staphylococcus aureus (ATCC 6538) 3rd generation (provided by Shanghai Beisi Biotechnology Co., Ltd.);

[0098] Escherichia coli (8099) 3rd generation (provided by Shanghai Beisi Biotechnology Co., Ltd.); Candida albicans (ATCC10231) 3rd generation (provided by Shanghai Beisi Biotechnology Co., Ltd.);

[0099] Antibacterial test procedure:

[0100] (1) Melt the agar medium and pour it into a plate.

[0101] (2) Take 0.2 mL of the bacterial solution to be tested and mix it evenly in LB semi-solid medium. Immediately pour it onto agar medium and shake well so that the upper layer of medium covers the plate.

[0102] (3) Using sterile forceps, immerse sterile filter paper in the antibacterial liquid to be tested, and then place it on a sterile agar plate. Record and control the contact time between the filter paper immersed in the antibacterial liquid and the agar plate for 2, 5, 10, and 20 minutes, respectively. Use sterile saline filter paper as a control. The experimental group immersed in the antibacterial liquid to be tested is designated as the experimental group; the experimental group immersed in sterile saline and placed on a sterile agar plate is designated as the positive control group; and the experimental group immersed in sterile saline but not placed on a sterile agar plate is designated as the negative control group.

[0103] (4) Place the plate with the attached filter paper in a 37°C incubator and incubate for 1 day.

[0104] (5) The OD values ​​of each experimental group were tested separately, and the antibacterial rate was obtained according to formula (1). The antibacterial test results of the plant dew prepared by using aged willow leaves, aged white honeysuckle leaves, and aged honeysuckle as raw materials are shown in Tables 7, 8 and 9 respectively.

[0105] Table 7. Antibacterial effect of plant dew prepared from dried willow leaves and wintersweet leaves on test bacteria.

[0106]

[0107]

[0108] Note: Negative controls showed no bacterial growth.

[0109] Table 8. Antibacterial effect of plant extract prepared with aged Baihao Yinzhen on test bacteria.

[0110]

[0111] Note: Negative controls showed no bacterial growth.

[0112] Table 9. Antibacterial effect of plant extract prepared from honeysuckle on test bacteria.

[0113]

[0114] Note: Negative controls showed no bacterial growth.

[0115] The interaction formula between aged willow leaves, aged wax plum leaves, aged white honeysuckle and aged honeysuckle was calculated using the Jin Zhengjun Q-value method, as shown in formula (2):

[0116]

[0117] Among them, E a+b+c The antibacterial rate of the plant extract prepared by using aged willow leaves, wintersweet leaves, aged white honeysuckle, and aged honeysuckle was measured by E. a E b and E c The antibacterial rates of the plant extracts prepared by using aged willow leaves, wintersweet leaves, aged white honeysuckle, and aged honeysuckle individually are shown in Table 10. The numerator represents the "measured combined effect," the denominator represents the "expected combined effect," and Q is the ratio of the two. If Q < 0.85, it indicates an antagonistic effect; if 0.85 ≤ Q < 1.15, it indicates an additive effect. The Q values ​​of the antibacterial rates of the plant extract prepared in Example 10 against the test bacteria compared to the plant extracts prepared by using aged willow leaves, wintersweet leaves, aged white honeysuckle, and aged honeysuckle individually are shown in Table 10.

[0118] Table 10 shows the Q-values ​​of the antibacterial rate of the plant dew based on *Chimonanthus praecox* prepared in Example 10 compared to the plant dew prepared using only aged *Chimonanthus praecox* leaves, aged *Silver Needle indicum*, and aged honeysuckle.

[0119]

[0120] The results showed that the plant dew prepared using dried willow-leaf wintersweet leaves, dried white honeysuckle, and dried honeysuckle alone also had a certain antibacterial effect, but all were lower than the antibacterial effect of the plant dew based on willow-leaf wintersweet prepared using the three raw materials in Example 10. Furthermore, the results calculated by Jin Zhengjun's Q-value method (Q values ​​were all greater than 1.15) showed that dried willow-leaf wintersweet leaves, dried white honeysuckle, and dried honeysuckle played a synergistic role in the later-prepared plant dew, improving the antibacterial effect of the dew.

[0121] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A plant dew based on Ledum groenlandicum, characterized in that, The raw material composition of the plant dew based on Ledum palustre according to weight parts comprises: 8-12 parts of old Ledum palustre leaves, 1-3 parts of old Baihao Yinzhen, 0.5-1.5 parts of old Lonicera japonica, 40-60 parts of deionized water and 1.8-3.2 parts of anhydrous ethanol; the composition of the old Ledum palustre leaves comprises old Ledum palustre spring leaves and old Ledum palustre autumn leaves, and the mass ratio of the two is 1-3:1-3; the plant dew based on Ledum palustre has the effects of eliminating inflammation and killing bacteria, and can be used for skin discomfort, oral cavity discomfort and throat discomfort. The preparation method of the old Ledum palustre leaves comprises the following steps: S1: wilting: select fresh leaves of Chimonanthus praecox and divide them into spring leaves and autumn leaves according to the picking time, and evenly spread them in the container, with a density of 10~20 kg / m 2 Then expose them to sunlight for 2~3 days to ensure that the moisture content of the Chimonanthus praecox is 60~80wt%. S2 drying: the Ledum palustre spring leaves and the Ledum palustre autumn leaves are dried by different processes respectively, so that the water content of the Ledum palustre spring leaves and the Ledum palustre autumn leaves is lower than 5 wt%; S3 aging: the stems of the dried Ledum palustre spring leaves and the Ledum palustre autumn leaves are removed, and the Ledum palustre spring leaves and the Ledum palustre autumn leaves are aged separately at a temperature of 20-30 °C and a relative humidity of 40%-60% for 3-5 years to obtain the old Ledum palustre spring leaves and the old Ledum palustre autumn leaves, and the obtained old Ledum palustre spring leaves and the old Ledum palustre autumn leaves are mixed according to a formula to obtain the old Ledum palustre leaves; The preparation method of the old Baihao Yinzhen comprises the following steps: S4 withering: picking white-blossom silver needle, and evenly spreading it in the utensil respectively, the density is 10-20 kg / m 2 Then it is exposed to sunlight for airing, and the airing time is 2-3 days, and the water content of the white-blossom silver needle is ensured to be 50-70 wt%. S5 drying: the withered Baihao Yinzhen is dried at a temperature of 25-30 °C and a relative humidity of 50%-60% for 12-24 hours, so that the water content of the dried Baihao Yinzhen is lower than 5 wt%; S6 aging: the stems of the dried Baihao Yinzhen are removed, and the Baihao Yinzhen is aged separately at a temperature of 20-30 °C and a relative humidity of 40%-60% for 3-5 years to obtain the old Baihao Yinzhen; The preparation method of the old Lonicera japonica comprises the following steps: S7 wilting: the Lonicera japonica is selected and placed in a high-temperature kettle, and is treated at a temperature of 70-80 °C and a turning speed of 10-20 turns / minute for 1-3 hours to ensure that the water content of the Lonicera japonica is 50-60 wt%; S8 drying: the wilted Lonicera japonica is dried at a temperature of 25-30 °C and a relative humidity of 40%-60% for 24-36 hours, so that the water content of the dried Lonicera japonica is lower than 5 wt%; S9 aging: the stems of the dried Lonicera japonica are removed, and the Lonicera japonica is aged separately at a temperature of 20-30 °C and a relative humidity of 40%-60% for 3-5 years to obtain the old Lonicera japonica.

2. A plant dew based on Ledum groenlandicum according to claim 1, characterized in that, The drying process of the Ledum palustre spring leaves in S2 is as follows: the wilted Ledum palustre spring leaves are dried at a temperature of 20-25 °C and a relative humidity of 67%-80% for 8-12 hours in the first stage; then the temperature is adjusted to 27-28 °C and the relative humidity is adjusted to 50%-60% for the second stage drying, and the drying time is 8-12 hours, so that the water content of the dried Ledum palustre spring leaves is lower than 5 wt%.

3. A plant dew based on Ledum groenlandicum according to claim 1, characterized in that, The drying process of the fall leaves of Ledum palustre in S2 is as follows: the wilted fall leaves of Ledum palustre are subjected to first-stage drying at a temperature of 30-32°C and a relative humidity of 60%-75% for 8-12 hours; then the temperature is adjusted to 35-37°C and the relative humidity is adjusted to 50%-55% for second-stage drying for 8-12 hours, so that the moisture content of the dried fall leaves of Ledum palustre is less than 5wt%.

4. The preparation method of the plant dew based on Leptodermum according to any one of claims 1-3, characterized in that, The preparation method comprises the following steps: S10 extraction of plant dew: according to the formula, the old Ledum palustre leaves, old white hair silver needle and old honeysuckle are added into a Soxhlet extractor, deionized water and anhydrous ethanol are mixed and added into the Soxhlet extractor, a reaction kettle at the lower end of the Soxhlet extractor is started to heat, the temperature is maintained at 78-90°C, and the reaction is carried out for 3-9 hours; the color of the liquid refluxed from the Soxhlet extractor into the reaction kettle is observed, and when the liquid color is colorless, the heating is stopped, and the extraction liquid in the reaction kettle is collected; S11 purification of the plant dew based on Ledum palustre: the collected extraction liquid is allowed to stand until the extraction liquid is stratified, and the water phase at the lower end is collected to obtain the plant dew based on Ledum palustre; S12 concentration adjustment of the plant dew based on Ledum palustre: the plant dew based on Ledum palustre is adjusted according to the required concentration by adding water, and then is added into a filling machine for filling.

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