A traditional Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain and its preparation process

Abstract

The invention discloses a traditional Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain and a preparation process thereof. The traditional Chinese medicine ointment comprises a traditional Chinese medicine liquid and a matrix, wherein the traditional Chinese medicine liquid comprises 90-110 parts of rhubarb, 90-110 parts of scutellaria baicalensis seeds, 90-110 parts of hemp seeds, 90-110 parts of radish seeds, 70-90 parts of cardamom, 90-110 parts of angelica, and 5-15 parts of cloves by weight, wherein the cardamom and cloves in the traditional Chinese medicine liquid are fermented by a composite bacterial agent. The composite bacterial strain comprises Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074. The matrix comprises the following raw materials by weight: 10.0-20.0 parts of glyceryl monostearate, 10.0-20.0 parts of paraffin, 50.0-70.0 parts of liquid paraffin, 5.0-7.0 parts of white vaseline, 0.3-0.5 parts of Span 80, 0.5-1.0 parts of OP emulsifier, 0.1-0.3 parts of chlorocresol, and 25.0-30.0 parts of distilled water. After cardamom and clove are fermented with specific Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074, they work synergistically with angelica sinensis, and have the effects of eliminating accumulation, relieving constipation, sedation, analgesia, and improving sleep.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain and a preparation process thereof. Background Art

[0002] There are many ways to treat constipation, relieve pain, improve sleep and other problems on the market, mainly oral medications, but there are certain problems in terms of cost, efficacy and elimination of side effects. Traditional Chinese medicine has a long history of external treatment, and plasters work through the skin. Plaster therapy is one of the commonly used external treatment methods in clinical Chinese medicine. It follows the principles of Chinese medicine syndrome differentiation and treatment and the efficacy, main indications and meridians of Chinese medicine, fully mobilizes the effectiveness of drugs to coordinate with each other, and forms a compound of multiple drugs to exert the good effect of drugs. Plasters are external pain relief products that act directly on the skin, reducing a series of adverse reactions caused by oral drugs. Chinese medicine also has the advantages of low price, long maintenance time, basically no side effects, convenience, safety, and reduced gastrointestinal irritation.

[0003] Based on the many advantages of traditional Chinese medicine, the research and development team developed a traditional Chinese medicine ointment through the combination of traditional Chinese medicine and Chinese herbal fermentation technology. Through the microbial fermentation of cardamom and cloves, and then synergistically with traditional Chinese medicines such as rhubarb, scutellaria baicalensis, hemp seed, radish seed, and angelica, it has the effects of eliminating accumulation and promoting bowel movements, calming and relieving pain, and improving sleep. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Chinese medicinal ointment for eliminating accumulation, relieving constipation and relieving pain and a preparation process thereof, so as to solve the problems raised in the above technical background.

[0005] To achieve the above object, the present invention is implemented through the following technical solutions:

[0006] In a first aspect, the present invention provides a Chinese medicinal ointment for eliminating accumulation, relieving constipation and relieving pain, comprising a Chinese medicinal liquid and a matrix, wherein the Chinese medicinal liquid comprises, by weight, 90-110 parts of rhubarb, 90-110 parts of scutellaria baicalensis seeds, 90-110 parts of hemp seeds, 90-110 parts of radish seeds, 70-90 parts of cardamom, 90-110 parts of angelica, and 5-15 parts of cloves, wherein the cardamom and cloves in the Chinese medicinal liquid are fermented by a composite bacterial agent.

[0007] The Chinese medicine liquid comprises 100 parts of rhubarb, 100 parts of scutellaria baicalensis seeds, 100 parts of hemp seeds, 100 parts of radish seeds, 80 parts of cardamom seeds, 100 parts of angelica sinensis and 10 parts of cloves by weight.

[0008] The composite strain comprises Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074. Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074 were purchased from Shanghai Chen Microbiology Technology Co., Ltd.

[0009] The matrix comprises the following raw materials by weight: 10.0-20.0 parts of glyceryl monostearate, 10.0-20.0 parts of paraffin, 50.0-70.0 parts of liquid paraffin, 5.0-7.0 parts of white vaseline, 0.3-0.5 parts of Span 800, 0.5-1.0 parts of OP emulsifier, 0.1-0.3 parts of chlorocresol, and 25.0-30.0 parts of distilled water. The liquid paraffin is heavy liquid paraffin.

[0010] The matrix comprises the following raw materials by weight: 10.0 parts of glyceryl monostearate, 10.0 parts of paraffin, 50.0 parts of liquid paraffin (heavy), 5.0 parts of white vaseline, 0.3 parts of Span 800, 0.5 parts of OP emulsifier, 0.1 parts of chlorocresol, and 25.0 parts of distilled water.

[0011] In the preparation process of the Chinese medicine liquid, rhubarb, scutellaria baicalensis, hemp seed and radish seed are mixed, then extracted with water and concentrated to a relative density of 1.1-1.2 (60° C.), finally ethanol is used for alcohol precipitation, the supernatant is filtered, and the filtrate is concentrated until there is no alcohol taste.

[0012] In the preparation process of the traditional Chinese medicine liquid, cardamom and clove are fermented by a composite bacterial agent to obtain a fermented product, and then the fermented product and angelica are mixed, extracted with alcohol, filtered to obtain a filtrate, and concentrated to have no alcohol taste.

[0013] The concentration of the Lactobacillus rhamnosus CICC 21006 bacterial solution is 4.8×10 8 ~1.0×10 9 cfu / mL, the concentration of Lactobacillus acidophilus CICC 6074 bacterial solution was 6.5×10 8 ~1.2×10 9 cfu / mL; the amount of Lactobacillus rhamnosus CICC 21006 culture solution added is 5-10% of the total mass of cardamom and clove, and the amount of Lactobacillus acidophilus CICC 6074 culture solution added is 5-10% of the total mass of cardamom and clove. 5-10% refers to the volume / mass ratio, for example, 100g of the total mass of cardamom and clove is inoculated with 5-10ml of Lactobacillus rhamnosus CICC 21006 culture solution or Lactobacillus acidophilus CICC 6074 culture solution.

[0014] In a second aspect, the present invention provides a method for preparing the above-mentioned traditional Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain, comprising the following steps:

[0015] S1. Material pretreatment: according to the formula, rhubarb, scutellaria baicalensis, hemp seed, radish seed, cardamom, angelica, and clove were weighed and crushed into 80-150 mesh powder respectively;

[0016] S2, take rhubarb, scutellaria baicalensis, hemp seed, radish seed, add 10 times the amount of water, decoct for 40 minutes, filter, and the filtrate is for use; the residue, continue to add 8 times the amount of water, decoct for 30 minutes, filter, and the filtrate is for use; the residue, continue to add 6 times the amount of water, decoct for 30 minutes, filter, discard the residue, and the filtrate is combined with the above two results, and concentrated to a relative density of 1.1-1.2 (60°C); put to room temperature, add an appropriate amount of 95% ethanol, adjust the alcohol content to 70%, seal, maintain the temperature at 16°C, and let stand for 48 hours.

[0017] The supernatant is filtered and the filtrate is concentrated until there is no alcohol taste, and the first concentrated solution is obtained for use;

[0018] S3, prepared at a concentration of 4.8×10 8 ~1.0×10 9 cfu / mL of Lactobacillus rhamnosus CICC 21006 and a concentration of 6.5×10 8 ~1.2×10 9 cfu / mL of Lactobacillus acidophilus CICC 6074 bacterial solution;

[0019] Preparation of Lactobacillus rhamnosus CICC 21006 bacterial solution: Lactobacillus rhamnosus CICC 21006 was activated as usual, and the activated Lactobacillus rhamnosus CICC 21006 was inoculated into a shake flask containing 500-1000 mL of MRS liquid medium and fermented for 8-12 hours to obtain a concentration of 4.8×10 8 ~1.0×10 9 cfu / mL of Lactobacillus rhamnosus CICC 21006 bacterial solution. Preparation of Lactobacillus acidophilus CICC 6074 bacterial solution: Lactobacillus acidophilus CICC 6074 was activated as usual, and the activated Lactobacillus acidophilus was inoculated into a shake flask containing 500-1000mL MRS liquid culture medium and fermented for 8-12h to obtain a concentration of 6.5×10 8 ~1.2×10 9 cfu / mL of Lactobacillus acidophilus liquid;

[0020] MRS medium: casein peptone 10.0 g, beef extract 10.0 g, yeast powder 5.0 g, glucose 5.0 g, sodium acetate 5.0 g, diammonium citrate 2.0 g, Tween 80 1.0 g, K2HPO4 2.0 g, MgSO4.7H2O 0.2 g, MnSO4.H2O 0.05 g, CaCO3 20.0 g, agar 15.0 g, distilled water 1.0 L, pH 6.8.

[0021] S4, taking cardamom and clove, adding Lactobacillus acidophilus CICC 6074 at an inoculum amount of 5-10%, mixing and fermenting in a cylinder, anaerobic fermentation at a temperature of 37° C. for 6 to 18 hours; then adding the same volume of Lactobacillus rhamnosus CICC 21006 bacterial liquid as that of Lactobacillus acidophilus CICC 6074, aerobic fermentation at a temperature of 37° C. for 6 to 18 hours, and after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074, to obtain a fermentation product;

[0022] S5, take the fermented product obtained in S4 and mix it with Chinese angelica, add 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate it until there is no alcohol taste, to obtain the second concentrated solution for use;

[0023] S6, mixing the first concentrated solution obtained in S2 and the second concentrated solution obtained in S5 to obtain a Chinese medicine solution;

[0024] S7. Preparation of matrix: put glyceryl monostearate and paraffin in an evaporating dish, heat and melt in a water bath, then add white vaseline, liquid paraffin and Span 80, and keep the temperature at 80°C until they are completely melted; add OP emulsifier and chlorocresol aqueous solution at the same temperature to the above oil phase solution, stirring continuously in the forward direction while adding, until it solidifies into a milky white semi-solid state;

[0025] S8, preparation of ointment film: 4 parts of the matrix obtained in S7 and 1 part of the Chinese medicine liquid obtained in S6 are mixed, glycerol is added, and the total weight is adjusted to 1 kg, and the mixture is placed in a high-pressure homogenizer, and homogenized for 60 minutes under the conditions of a flow rate of 0.1 T / H and a pressure of 25 mPa, and the ointment is obtained by bottling. The amount of glycerol added is 5-8% of the ointment.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] 1. After the cardamom and cloves contained in the traditional Chinese medicine liquid are fermented with specific Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074, they work synergistically with other components of the traditional Chinese medicine liquid. The resulting traditional Chinese medicine ointment has significantly enhanced effects on eliminating accumulation, relieving constipation, and sedation and analgesia, which is much higher than the traditional Chinese medicine ointment obtained by fermenting the cardamom and cloves contained in the traditional Chinese medicine liquid with a single bacterial agent, or one of the strains is fermented with other Lactobacillus rhamnosus and other Lactobacillus acidophilus together with other components of the traditional Chinese medicine liquid.

[0028] 2. After cardamom and cloves are fermented with specific Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074, they work synergistically with angelica to improve sleep.

[0029] 3. The Chinese medicinal ointment obtained by the present invention can be applied externally, applied, massaged, etc., has no side effects, is convenient, safe, and reduces gastrointestinal irritation. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The figure is a bar graph of the ink advancement rate of each group of mice in the present invention;

[0031] Figure 2 A bar graph showing the number of writhing times of mice in each group of the present invention;

[0032] Figure 3 It is a bar graph of the prolonged sleep time and sleep latency of each group of mice in the present invention.

[0033] Figure 4 The real object of the Chinese medicinal ointment obtained in Example 2 Figure 1 ;

[0034] Figure 5 The real object of the Chinese medicinal ointment obtained in Example 2 Figure 2 . DETAILED DESCRIPTION

[0035] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0036] In the present invention, the rhubarb, scutellaria baicalensis seeds, hemp seeds, radish seeds, cardamom, angelica and cloves used are all purchased from the market, and the quality requirements all meet the standards of the 2015 edition of the Pharmacopoeia.

[0037] Example 1

[0038] The present embodiment provides a Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain, including a Chinese medicine liquid and a matrix, wherein the Chinese medicine liquid contains 90g of rhubarb, 110g of scutellaria baicalensis, 110g of hemp seed, 90g of radish seed, 90g of cardamom, 110g of angelica sinensis, and 5g of cloves, wherein the cardamom and cloves in the Chinese medicine liquid are fermented by a composite bacterial agent. The composite strain contains Lactobacillus rhamnosus CICC21006 and Lactobacillus acidophilus CICC 6074. The matrix contains the following raw materials: 10.0g of glyceryl monostearate, 10.0g of paraffin, 50.0g of liquid paraffin (heavy), 5.0g of white vaseline, 0.3g of Span 800, 0.5g of OP emulsifier, 0.1g of chlorocresol, and 25.0g of distilled water.

[0039] The present embodiment provides a Chinese medicinal ointment for eliminating accumulation, relieving constipation and relieving pain, and the preparation method thereof comprises the following steps:

[0040] S1. Material pretreatment: according to the formula, 90 g of rhubarb, 110 g of scutellaria baicalensis seeds, 110 g of hemp seeds, 90 g of radish seeds, 90 g of cardamom seeds, 110 g of angelica sinensis, and 5 g of cloves were weighed and crushed into 100-mesh powder respectively;

[0041] S2, get the rhubarb, scutellaria baicalensis, hemp seed, radish seed after S1 processing, add 10 times of water, decoct for 40 minutes, filter, and the filtrate is for standby; the residues, continue to add 8 times of water, decoct for 30 minutes, filter, and the filtrate is for standby; the residues, continue to add 6 times of water, decoct for 30 minutes, filter, the residues are discarded, the filtrate is merged with the above-mentioned two gained, and concentrated to a relative density of 1.1 (60 ℃); put to room temperature, add 95% ethanol in right amount, adjust the alcohol content to 70%, seal, maintain the temperature at 16 ℃, leave standstill for 48 hours, filter the supernatant, and the filtrate is concentrated to no alcohol taste, and the first concentrated solution is for standby;

[0042] S3, prepared at a concentration of 4.8×10 8 cfu / mL of Lactobacillus rhamnosus CICC 21006 and a concentration of 1.2×10 9 cfu / mL of Lactobacillus acidophilus CICC 6074 bacterial solution; preparation of Lactobacillus rhamnosus CICC 21006 bacterial solution: Lactobacillus rhamnosus CICC 21006 was activated as usual, and the activated Lactobacillus rhamnosus CICC 21006 was inoculated into a shake flask filled with 500 mL of MRS liquid culture medium and fermented for 8 hours to obtain a concentration of 4.8×10 8 cfu / mL of Lactobacillus rhamnosus CICC 21006 bacterial solution. Preparation of Lactobacillus acidophilus CICC 6074 bacterial solution: Lactobacillus acidophilus CICC 6074 was activated as usual, and the activated Lactobacillus acidophilus was inoculated into a shake flask containing 1000 mL of MRS liquid culture medium and fermented for 12 hours to obtain a concentration of 1.2×10 9 cfu / mL of Lactobacillus acidophilus liquid;

[0043] S4, taking cardamom and clove, adding Lactobacillus acidophilus CICC 6074 at an inoculum amount of 10%, mixing and fermenting in a cylinder, anaerobic fermentation, the temperature is 37 ° C, and the fermentation time is 6 hours; then adding Lactobacillus rhamnosus CICC 21006 bacterial solution at an inoculum amount of 5% of the total mass of cardamom and clove, aerobic fermentation, the temperature is 37 ° C, the fermentation time is 18 hours, after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074, and obtaining a fermentation product;

[0044] S5, taking the fermented product obtained in S4 and mixing it with the angelica sinensis treated in S1, adding 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, 3 times, discarding the residue, combining the filtrate, recovering the alcohol, and concentrating it until there is no alcohol taste, to obtain the second concentrated solution for use;

[0045] S6, mixing the first concentrated solution obtained in S2 and the second concentrated solution obtained in S5 to obtain a Chinese medicine solution;

[0046] S7. Preparation of matrix: According to the formula, weigh 10.0 g of glyceryl monostearate, 10.0 g of paraffin, and liquid paraffin (heavy)

[0047] 50.0g, white vaseline 5.0g, Span 800.3g, OP emulsifier 0.5g, chlorocresol 0.1g, distilled water 25.0g. Place glyceryl monostearate and paraffin in an evaporating dish, heat and melt them in a water bath, then add white vaseline, liquid paraffin, and Span 80, and keep the temperature at 80℃ until they are completely melted; add the OP emulsifier and chlorocresol aqueous solution at the same temperature into the above oil phase solution, stirring continuously in the forward direction while adding, until it solidifies into a milky white semi-solid state.

[0048] S8, preparation of ointment film: 4 parts of the matrix obtained in S7 and 1 part of the Chinese medicine liquid obtained in S6 are mixed, glycerol is added, and the total weight is adjusted to 1 kg, and the mixture is placed in a high-pressure homogenizer, and homogenized for 60 minutes under the conditions of a flow rate of 0.1 T / H and a pressure of 25 mPa, and the Chinese medicine ointment is obtained by bottling. The amount of glycerol added is 8% of the Chinese medicine ointment.

[0049] Example 2

[0050] The present embodiment provides a Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain, including a Chinese medicine liquid and a matrix, wherein the Chinese medicine liquid contains 100g of rhubarb, 100g of scutellaria baicalensis, 100g of hemp seed, 100g of radish seed, 80g of cardamom, 100g of angelica, and 10g of clove, wherein the cardamom and clove in the Chinese medicine liquid are fermented by a composite bacterial agent. The composite strain contains Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074. The matrix contains the following raw materials: 10.0g of glyceryl monostearate, 10.0g of paraffin, 50.0g of liquid paraffin (heavy), 5.0g of white vaseline, 0.3g of Span 800, 0.5g of OP emulsifier, 0.1g of chlorocresol, and 25.0g of distilled water.

[0051] The present embodiment provides a Chinese medicinal ointment for eliminating accumulation, relieving constipation and relieving pain, and the preparation method thereof comprises the following steps:

[0052] S1. Material pretreatment: according to the formula, 100 g of rhubarb, 100 g of scutellaria baicalensis, 100 g of hemp seed, 100 g of radish seed, 80 g of cardamom, 100 g of angelica, and 10 g of clove were weighed and crushed into 100-mesh powder respectively;

[0053] S2, get the rhubarb, scutellaria baicalensis, hemp seed, radish seed after S1 processing, add 10 times of water, decoct for 40 minutes, filter, and the filtrate is for standby; the residues, continue to add 8 times of water, decoct for 30 minutes, filter, and the filtrate is for standby; the residues, continue to add 6 times of water, decoct for 30 minutes, filter, the residues are discarded, the filtrate is merged with the above-mentioned two gained, and concentrated to a relative density of 1.2 (60 ℃); put to room temperature, add 95% ethanol in right amount, adjust the alcohol content to 70%, seal, maintain the temperature at 16 ℃, leave standstill for 48 hours, filter the supernatant, and the filtrate is concentrated to no alcohol taste, and the first concentrated solution is for standby;

[0054] S3, prepared at a concentration of 8.0×10 8 cfu / mL of Lactobacillus rhamnosus CICC 21006 and a concentration of 1.0×10 9 cfu / mL of Lactobacillus acidophilus CICC 6074 bacterial solution;

[0055] Preparation of Lactobacillus rhamnosus CICC 21006 bacterial solution: Lactobacillus rhamnosus CICC 21006 was activated as usual, and the activated Lactobacillus rhamnosus CICC 21006 was inoculated into a shake flask filled with 800 mL of MRS liquid medium and fermented for 10 h to obtain a concentration of 8.0 × 10 8 cfu / mL of Lactobacillus rhamnosus CICC 21006 bacterial solution. Preparation of Lactobacillus acidophilus CICC 6074 bacterial solution: Lactobacillus acidophilus CICC 6074 was activated as usual, and the activated Lactobacillus acidophilus was inoculated into a shake flask containing 800 mL of MRS liquid culture medium and fermented for 10 h to obtain a concentration of 1.0×10 9 cfu / mL of Lactobacillus acidophilus liquid;

[0056] S4, taking cardamom and clove, adding Lactobacillus acidophilus CICC 6074 at an inoculum amount of 8%, mixing and fermenting in a cylinder, anaerobic fermentation, the temperature is 37 ° C, and the fermentation time is 12 hours; then adding Lactobacillus rhamnosus CICC 21006 bacterial solution at an inoculum amount of 8% of the total mass of cardamom and clove, aerobic fermentation, the temperature is 37 ° C, the fermentation time is 12 hours, after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074, and obtaining a fermentation product;

[0057] S5, taking the fermented product obtained in S4 and mixing it with the angelica sinensis treated in S1, adding 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, 3 times, discarding the residue, combining the filtrate, recovering the alcohol, and concentrating it until there is no alcohol taste, to obtain the second concentrated solution for use;

[0058] S6, mixing the first concentrated solution obtained in S2 and the second concentrated solution obtained in S5 to obtain a Chinese medicine solution;

[0059] S7. Preparation of matrix: According to the formula, weigh 10.0 g of glyceryl monostearate, 10.0 g of paraffin, and liquid paraffin (heavy)

[0060] 50.0g, white vaseline 5.0g, Span 800.3g, OP emulsifier 0.5g, chlorocresol 0.1g, distilled water 25.0g. Place glyceryl monostearate and paraffin in an evaporating dish, heat and melt them in a water bath, then add white vaseline, liquid paraffin, and Span 80, and keep the temperature at 80℃ until they are completely melted; add the OP emulsifier and chlorocresol aqueous solution at the same temperature into the above oil phase solution, stirring continuously in the forward direction while adding, until it solidifies into a milky white semi-solid state.

[0061] S8, preparation of ointment film: 4 parts of the matrix obtained in S7 and 1 part of the Chinese medicine solution obtained in S6 are mixed, glycerin is added, and the total weight is adjusted to 1 kg. The mixture is placed in a high-pressure homogenizer, and the flow rate is 0.1 T / H and the pressure is 25 mPa. Homogenize for 60 minutes, and bottle the ointment. The actual product is shown in the figure below. Figure 4 , Figure 5 As shown, the added amount of glycerol is 8% of the traditional Chinese medicine ointment.

[0062] Example 3

[0063] The present embodiment provides a Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain, including a Chinese medicine liquid and a matrix, wherein the Chinese medicine liquid contains 110g of rhubarb, 90g of scutellaria baicalensis, 90g of hemp seed, 110g of radish seed, 70g of cardamom, 90g of angelica sinensis, and 15g of cloves, wherein the cardamom and cloves in the Chinese medicine liquid are fermented by a composite bacterial agent. The composite strain contains Lactobacillus rhamnosus CICC21006 and Lactobacillus acidophilus CICC 6074. The matrix contains the following raw materials: 10.0g of glyceryl monostearate, 10.0g of paraffin, 50.0g of liquid paraffin (heavy), 5.0g of white vaseline, 0.3g of Span 800, 0.5g of OP emulsifier, 0.1g of chlorocresol, and 25.0g of distilled water.

[0064] The present embodiment provides a Chinese medicinal ointment for eliminating accumulation, relieving constipation and relieving pain, and the preparation method thereof comprises the following steps:

[0065] S1. Material pretreatment: according to the formula, 110 g of rhubarb, 90 g of scutellaria baicalensis, 90 g of hemp seed, 110 g of radish seed, 70 g of cardamom, 90 g of angelica sinensis, and 15 g of clove were weighed and crushed into 100 mesh powder respectively;

[0066] S2, get the rhubarb, scutellaria baicalensis, hemp seed, radish seed after S1 processing, add 10 times of water, decoct for 40 minutes, filter, and the filtrate is for standby; the residues, continue to add 8 times of water, decoct for 30 minutes, filter, and the filtrate is for standby; the residues, continue to add 6 times of water, decoct for 30 minutes, filter, the residues are discarded, the filtrate is merged with the above-mentioned two gained, and concentrated to a relative density of 1.2 (60 ℃); put to room temperature, add 95% ethanol in right amount, adjust the alcohol content to 70%, seal, maintain the temperature at 16 ℃, leave standstill for 48 hours, filter the supernatant, and the filtrate is concentrated to no alcohol taste, and the first concentrated solution is for standby;

[0067] S3, prepared at a concentration of 1.0×10 9 cfu / mL of Lactobacillus rhamnosus CICC 21006 and a concentration of 6.5×10 8 cfu / mL of Lactobacillus acidophilus CICC 6074 bacterial solution;

[0068] Preparation of Lactobacillus rhamnosus CICC 21006 bacterial solution: Lactobacillus rhamnosus CICC 21006 was activated as usual, and the activated Lactobacillus rhamnosus CICC 21006 was inoculated into a shake flask containing 1000 mL of MRS liquid medium and fermented for 12 h to obtain a concentration of 1.0×10 9 cfu / mL of Lactobacillus rhamnosus CICC 21006 bacterial solution. Preparation of Lactobacillus acidophilus CICC 6074 bacterial solution: Lactobacillus acidophilus CICC 6074 was activated as usual, and the activated Lactobacillus acidophilus was inoculated into a shake flask containing 500 mL of MRS liquid culture medium and fermented for 8 hours to obtain a concentration of 6.5×10 8 cfu / mL of Lactobacillus acidophilus liquid;

[0069] S4, taking cardamom and clove, adding Lactobacillus acidophilus CICC 6074 at an inoculum amount of 5%, mixing and fermenting in a cylinder, anaerobic fermentation, the temperature is 37 ° C, and the fermentation time is 18 hours; then adding Lactobacillus rhamnosus CICC 21006 bacterial solution at an inoculum amount of 10% of the total mass of cardamom and clove, aerobic fermentation, the temperature is 37 ° C, the fermentation time is 6 hours, and after the fermentation is completed, 3% hydrogen peroxide is added to inactivate Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074 to obtain a fermentation product;

[0070] S5, taking the fermented product obtained in S4 and mixing it with the angelica sinensis treated in S1, adding 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, 3 times, discarding the residue, combining the filtrate, recovering the alcohol, and concentrating it until there is no alcohol taste, to obtain the second concentrated solution for use;

[0071] S6, mixing the first concentrated solution obtained in S2 and the second concentrated solution obtained in S5 to obtain a Chinese medicine solution;

[0072] S7. Preparation of matrix: According to the formula, weigh 10.0 g of glyceryl monostearate, 10.0 g of paraffin, and liquid paraffin (heavy)

[0073] 50.0g, white vaseline 5.0g, Span 800.3g, OP emulsifier 0.5g, chlorocresol 0.1g, distilled water 25.0g. Place glyceryl monostearate and paraffin in an evaporating dish, heat and melt them in a water bath, then add white vaseline, liquid paraffin, and Span 80, and keep the temperature at 80℃ until they are completely melted; add the OP emulsifier and chlorocresol aqueous solution at the same temperature into the above oil phase solution, stirring continuously in the forward direction while adding, until it solidifies into a milky white semi-solid state.

[0074] S8, preparation of ointment film: 4 parts of the matrix obtained in S7 and 1 part of the Chinese medicine liquid obtained in S6 are mixed, glycerol is added, and the total weight is adjusted to 1 kg, and the mixture is placed in a high-pressure homogenizer, and homogenized for 60 minutes under the conditions of a flow rate of 0.1 T / H and a pressure of 25 mPa, and the Chinese medicine ointment is obtained by bottling. The amount of glycerol added is 8% of the Chinese medicine ointment.

[0075] Comparative Example 1

[0076] This comparative example is similar to Example 2, except that only Lactobacillus rhamnosus CICC 21006 is used for fermentation in step S4; the rest is the same as Example 2.

[0077] Comparative Example 2

[0078] This comparative example is similar to Example 2, except that only Lactobacillus acidophilus CICC 6074 is used for fermentation in step S4; the rest is the same as Example 2.

[0079] Comparative Example 3

[0080] This comparative example is similar to Example 2, except that in step S4, Lactobacillus rhamnosus CICC 24869 is used to replace Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074 for fermentation; the rest is the same as Example 2. Lactobacillus rhamnosus CICC 24869 was purchased from Shanghai Chen Microbiology Technology Co., Ltd.

[0081] Comparative Example 4

[0082] This comparative example is similar to Example 2, except that in step S4, Lactobacillus acidophilus CICC 6005 is used to replace Lactobacillus acidophilus CICC 6074 and Lactobacillus rhamnosus CICC 21006 for fermentation; the rest is the same as Example 2. Lactobacillus acidophilus CICC 6005 was purchased from Shanghai Chen Microbiology Technology Co., Ltd.

[0083] Comparative Example 5

[0084] This comparative example is similar to Example 2, except that the cardamom and clove in step S4 are not fermented, but are directly mixed with angelica for alcohol extraction in step S5; the rest is the same as Example 2.

[0085] Comparative Example 6

[0086] This comparative example is similar to Example 2, except that in step S4, cardamom is removed, only cloves are used for fermentation, and cardamom is subjected to alcohol extraction together with the fermentation product and angelica in step S5; the rest is the same as Example 2.

[0087] Comparative Example 7

[0088] This comparative example is similar to Example 2, except that cloves are removed in step S4, only cardamom is used for fermentation, and cloves are subjected to alcohol extraction together with the fermentation product and angelica in step S5; the rest is the same as Example 2.

[0089] Comparative Example 8

[0090] This comparative example is similar to Example 2, except that Angelica sinensis is removed in step S5, and only the fermented product is used for alcohol extraction; the rest is the same as Example 2.

[0091] 1. Study on the effect of traditional Chinese medicine paste on eliminating accumulation and relieving constipation

[0092] 1.1 Mouse grouping: 180 SPF KM mice weighing 18-22 g were selected, half of which were male and half were female. The KM mice were purchased from Nanjing Junke Biotechnology Co., Ltd. After 1 week of adaptive feeding, the mice were randomly divided into two large groups, namely the small intestinal motility experiment group and the defecation experiment group. Each large group was divided into 9 small groups. There was no statistical difference between the mice in each group. 24 h before the experiment, all mice were depilated on their abdomens with 7% NaS, and the depilated area was about 1.5 cm 2 . In each large group, there are 9 groups, namely blank control group (matrix), positive control group (Maren Pill ig. 1.0g / kg·bw, intragastric administration volume is 10ml / kg·bw), model group (matrix), Example 2 and comparative examples 1 to 5 treatment groups (corresponding to the obtained Chinese medicine ointment), with 10 mice in each group. Among them, except for the normal mouse group as a blank control, other groups all used mice with successful constipation modeling; the matrix used in the blank control group and the model group was the matrix of S7 in Example 2 with glycerol added, and the amount of glycerol added was 8% of the total amount, and the total weight was adjusted to 1Kg, placed in a high-pressure homogenizer, with a flow rate of 0.1T / H and a pressure of 25mPa, and homogenized for 60min; Maren Pill, Hubei Nodesheng Pharmaceutical Co., Ltd.; Compound diphenoxylate, Changzhou Kangpu Pharmaceutical Co., Ltd.

[0093] 1. Small Intestinal Motility Test

[0094] Each group of mice was given medication according to the corresponding settings, and the test sample was continuously given for 7 days. After the last dose, the mice in each group were fasted but not watered for 16 hours. The mice in the positive control group, model group, and the treatment groups of Example 2 and Comparative Examples 1 to 5 were gavaged with compound diphenoxylate (ig. 10 mg / kg·bw, gavage volume was 10 ml / kg·bw), and the blank control group was given an equal dose of distilled water to establish a constipation model. 30 minutes after modeling, the positive control group was gavaged with ink containing the corresponding test sample, and the blank control group, model group, and the treatment groups of Example 2 and Comparative Examples 1 to 5 were gavaged with the same dose of ink. At the same time, the blank control group and the model group were gavaged with an area of ​​1.5 cm 2 , 1mm thick matrix was applied to the depilatory area of ​​the mouse abdomen, and the application area of ​​the treatment groups of Example 2 and Comparative Examples 1 to 5 was 1.5cm 2 , 1mm thick, and the corresponding Chinese medicine paste was applied to the depilated part of the mouse abdomen. 25 minutes after the ink was given, all mice in the small intestinal motility experimental group were immediately killed by pulling the neck, dissected, the abdominal cavity was opened, the mesentery was separated, and the intestinal tube part from the pylorus at the upper end and the ileum at the lower end was cut and placed on a tray. The small intestine was gently pulled into a straight line, and the intestinal tube length was measured and recorded as the "total length of the small intestine". The length from the pylorus to the front of the ink was recorded as the "ink propulsion length". The ink propulsion rate was calculated according to the following formula to judge the effect on the small intestinal peristalsis of constipated mice. The test results are shown in Tables 1 and Figure 1 shown.

[0095] Ink propagation rate (%) = (ink propagation length / total length of small intestine) × 100%

[0096] 2. Defecation experiment

[0097] Each group of mice was given medication according to the corresponding settings, and the test sample was continuously given for 7 days. After the last dose, the mice in each group were fasted but not watered for 16 hours. The mice in the positive control group, model group, and the treatment groups of Example 2 and Comparative Examples 1 to 5 were gavaged with compound diphenoxylate (ig. 10 mg / kg·bw, gavage volume was 10 ml / kg·bw), and the blank control group was given an equal dose of distilled water to establish a constipation model. 30 minutes after modeling, the positive control group was gavaged with ink containing the corresponding test sample, and the blank control group, model group, and the treatment groups of Example 2 and Comparative Examples 1 to 5 were gavaged with the same dose of ink. At the same time, the blank control group and the model group were gavaged with an area of ​​1.5 cm 2 , 1mm thick matrix was applied to the depilatory area of ​​the mouse abdomen, and the application area of ​​the treatment groups of Example 2 and Comparative Examples 1 to 5 was 1.5cm 2 , 1mm thick, the corresponding Chinese medicine paste was applied to the depilated part of the mouse abdomen. Each mouse in the defecation experimental group was housed in a single cage, and had normal food, water and activities. Starting from the intragastric administration of ink, the time of the first black stool of each mouse, the number and weight of black stool within 6 hours were observed and recorded, and the fecal water content was calculated according to the following formula to judge the effect on the defecation of constipated mice. The experimental results are shown in Table 2.

[0098] Fecal moisture content (%) = [(fecal wet weight - fecal dry weight) / fecal wet weight] × 100%

[0099] Table 1

[0100]

[0101]

[0102] Table 2

[0103]

[0104] From Table 1 and Figure 1 It can be seen that compared with the blank control group, the small intestinal ink propulsion rate of the model group mice was significantly reduced, indicating that the model was successfully constructed. Compared with the model group, the small intestinal ink propulsion rate of the positive control group was significantly increased (P<0.05), indicating that the experimental system is feasible; compared with comparative examples 1-5, the small intestinal ink propulsion rate of Example 2 was significantly increased.

[0105] As shown in Table 2, compared with the blank control group, the first defecation time of the model group was significantly increased, the average number of black stool particles within 6 hours was significantly reduced, and the average weight of black stool within 6 hours was reduced, indicating that the model was successfully established. Compared with the model group, the first defecation time of the positive control group was significantly reduced, and the average number of black stool particles within 6 hours and the average weight of black stool within 6 hours were significantly increased, indicating that the experimental system is feasible. Compared with comparative examples 1-5, the first defecation time of Example 2 was significantly reduced, and the average number of black stool particles within 6 hours and the average weight of black stool within 6 hours were significantly increased.

[0106] As can be seen from the above, the effect of eliminating accumulation and relieving constipation in Example 2 is significantly higher than that in Comparative Examples 1-5. This is because the cardamom and cloves contained in the traditional Chinese medicine liquid are fermented by specific Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074, and the other components of the obtained traditional Chinese medicine liquid work synergistically, and the obtained traditional Chinese medicine ointment has a significantly enhanced effect of eliminating accumulation and relieving constipation, which is much higher than the traditional Chinese medicine ointment obtained by fermenting the cardamom and cloves contained in the traditional Chinese medicine liquid with a single bacterial agent, or one of the two strains with other Lactobacillus rhamnosus and other Lactobacillus acidophilus together with other components of the obtained traditional Chinese medicine liquid.

[0107] 2. Study on the sedative and analgesic effects of Chinese herbal ointment

[0108] The sedation and analgesia experiment was measured by the acetic acid-induced mouse writhing test.

[0109] Experimental grouping: 100 SPF grade Kunming mice were selected, half male and half female, weighing 20±2g. 100 mice were randomly divided into 10 groups, 10 mice in each group, respectively, blank control group (matrix), positive control group (diclofenac diethylamine emulsion (Voltaren)), Example 1-3 group and comparative example 1-5 group (corresponding to the obtained Chinese medicine ointment). The mice and feed used in this experiment were purchased from Nanjing Junke Bioengineering Co., Ltd. The matrix used in the blank control group is the matrix of S7 in Example 2 with glycerol added, the amount of glycerol added is 8% of the total amount, the total weight is adjusted to 1Kg, placed in a high-pressure homogenizer, flow rate 0.1T / H, pressure 25mPa, homogenized for 60min; diclofenac diethylamine emulsion (Voltaren), 20g: 0.2g / stick, purchased from Beijing Novartis Pharmaceutical Co., Ltd.

[0110] 24 h before the experiment, the mice in each group were depilated on the back of the neck with 7% NaS, and the depilated area was about 1.5 cm 2 ; The corresponding drugs were applied to the back of the neck of each group of mice at an average thickness of 1 mm / mouse, twice a day, for 7 consecutive days. One hour after the last administration, 0.2 mL of 0.5% glacial acetic acid was injected intraperitoneally into each mouse, and then the number of twisting times of each mouse within 15 minutes was immediately observed, and the inhibition rate was calculated. The results are shown in Table 3 and Figure 2 shown.

[0111] Inhibition rate (%) = [(average number of twists in the blank control group - average number of twists in the experimental group) / average number of twists in the blank control group] × 100%

[0112] Table 3

[0113]

[0114] From Table 3 and Figure 2 It can be seen that compared with the blank control group, the number of twists in the positive control group, Examples 1-3 and Comparative Examples 1-5 was significantly reduced; compared with Comparative Examples 1-5, the number of twists in Example 2 was significantly reduced, indicating that the sedative and analgesic effect of Example 2 was significantly higher than that of Comparative Examples 1-5. This is because the cardamom and cloves contained in the traditional Chinese medicine liquid are fermented with specific Lactobacillus rhamnosus CICC21006 and Lactobacillus acidophilus CICC 6074, and the sedative and analgesic effect of the obtained traditional Chinese medicine ointment is significantly enhanced, which is much higher than the traditional Chinese medicine ointment obtained by fermenting the cardamom and cloves contained in the traditional Chinese medicine liquid with a single bacterial agent, or one of the two strains with other Lactobacillus rhamnosus and other Lactobacillus acidophilus together with other components of the obtained traditional Chinese medicine liquid.

[0115] 3. Research on the effect of treatment of insomnia

[0116] 3.1 Animal grouping: SPF male ICR mice were purchased from Nanjing Junke Biotechnology Co., Ltd., weighing 18-22 g. After one week of adaptive feeding, the mice were randomly divided into two experimental groups: sodium pentobarbital group and sodium pentobarbital group. Each experimental group was randomly divided into 12 groups. There was no statistical difference between the mice in each group. 24 h before the experiment, the mice in each group were depilated on the back of the neck with 7% NaS, and the depilated area was about 1.5 cm 2 Each group consisted of 12 blank control groups (matrix, smear area 1.5 cm 2 , 1 mm thick on the depilated area of ​​the mouse neck and back), positive control group (diazepam ig.2 mg / kg·bw, oral gavage volume of 10 ml / kg·bw), model group (matrix, smear area 1.5 cm 2 , 1mm thick on the depilatory area of ​​the mouse neck and back), Example 2 and Comparative Examples 1 to 8 treatment groups (corresponding to the obtained Chinese medicine ointment, the application area is 1.5cm 2 , 1mm thick on the depilatory part of the mouse neck and back), 10 mice in each group. Among them, except for the normal mouse group as a blank control, all other groups used PCPA modeling mice; the matrix used in the blank control group and the model group was the matrix of S7 in Example 2 with glycerol added, the amount of glycerol added was 8% of the total amount, the total weight was adjusted to 1Kg, placed in a high-pressure homogenizer, flow rate 0.1T / H, pressure 25mPa, homogenized for 60min;

[0117] 3.2 Animal modeling: SPF male ICR mice were adaptively fed for one week. On the 7th day of administration, mice were intraperitoneally injected with PCPA suspension (300 mg / kg) for 3 consecutive days. 24 to 30 hours after the first injection of PCPA, the model group showed obvious physiological rhythm reduction, daytime inactivity, malaise, increased aggression and other phenomena, which were significantly different from the control group, indicating that the insomnia model was successfully established. Preparation of PCPA suspension: Weigh PCPA powder and add weak alkaline saline (pH 7 to 8) for shaking, add appropriate amount of Tween 80 to promote dissolution, and ultrasonicate for 30 minutes to make a 30 mg / ml PCPA suspension. PCPA (C136727) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.; sodium pentobarbital and sodium barbital were purchased from Beijing Huaye Huanyu Chemical Co., Ltd.; diazepam tablets were purchased from Shanxi Angsheng Pharmaceutical Co., Ltd.

[0118] 3.3 Experiment on prolonging sleep time with sodium pentobarbital

[0119] Each group of mice was given the drug continuously for 14 days. 15 minutes after the last drug administration, each group of mice was intraperitoneally injected with sodium pentobarbital solution (38 mg / kg), with an injection volume of 0.1 mL / 10 g. The disappearance of the righting reflex of mice was used as an indicator of sleep. When mice were in a dorsal recumbent position, those that could not right themselves for more than 1 minute were considered to have entered a sleep state, and the recovery of the righting reflex was used as the end of sleep standard. The time from the disappearance of the righting reflex to the recovery of the righting reflex of each group of mice after the injection of sodium pentobarbital was recorded as the sleep duration. The results are shown in Tables 4 and Figure 3 shown.

[0120] 3.4 Sodium barbital sleep latency experiment

[0121] Each group of mice was given the drug by oral gavage for 14 consecutive days. Fifteen minutes after the last drug administration, each group of mice was intraperitoneally injected with sodium barbital solution (240 mg / kg) at an injection volume of 0.1 mL / 10 g. The disappearance of the righting reflex of mice was used as an indicator of sleep. When mice were in dorsal recumbency, those that could not right themselves for more than 1 minute were considered to have entered a sleep state, and the recovery of the righting reflex was used as the end of sleep standard. The sleep latency of each group of mice was recorded. The time from the injection of sodium barbital to the disappearance of the righting reflex of each group of mice was recorded as the sleep latency. The results are shown in Tables 4 and Figure 3 shown.

[0122] Table 4

[0123]

[0124]

[0125] From Table 4 and Figure 3 It can be seen that compared with the normal group, the extended sleep time of the mice in the model group was significantly shortened and the sleep latency was significantly prolonged. The results show that the PCPA mouse insomnia model was successfully established. Compared with the model group, the extended sleep time of the mice in Example 2 was significantly prolonged and the sleep latency was significantly shortened, while the difference between Comparative Examples 1-8 was not much, indicating that Comparative Examples 1-8 had no improvement effect or a small improvement effect on insomnia; compared with Comparative Examples 1-8, the extended sleep time of the mice in Example 2 was significantly prolonged and the sleep latency was significantly shortened, indicating that Example 2 has a significant effect of improving insomnia, because cardamom and cloves were fermented with specific rhamnosus Lactobacillus CICC 21006 and Lactobacillus acidophilus CICC 6074, and then synergized with Angelica sinensis, which has the effect of improving sleep.

[0126] Clinical Cases

[0127] Case 1, He * Ning, female, 7 years old, consultation time: 2024.5.7, consultation location: Beijing Kyoto Children's Hospital.

[0128] Chief complaint: Food indigestion (second visit)

[0129] Current medical history: After the child cooperated with massage, the indigestion improved, there was no fever, no wheezing, the general condition was good, the sleep was normal, the stool was dry, the urination was normal, and massage was performed.

[0130] Current medication history: Massage therapy

[0131] Past medical history: The patient was in good health, denied any history of infectious diseases or close contact with infectious diseases, denied any history of asthma, and denied any history of foreign body inhalation.

[0132] Physical examination: body temperature 36.4℃, pain assessment: none, high risk of falling: yes, intervention measures for falling: education, accompanied by family members. Conscious, mentally OK, stable breathing, no nasal flaring, no "three-depression sign", no rash, no bleeding spots, superficial lymph nodes not touched, no congestion of conjunctiva, no obvious congestion of pharynx, no enlargement of bilateral tonsils, no herpes, soft neck, symmetrical breath sounds of both lungs, slightly coarse breath sounds, no dry or wet rales, strong heart sounds, no murmurs, regular rhythm, soft abdomen, no tenderness, no rebound pain, warm extremities. Red tongue, white and greasy fur. Pulse: smooth and thin.

[0133] Preliminary diagnosis (or diagnosis): 1. Malnutrition syndrome 2. Indigestion 3. Food stagnation syndrome

[0134] Treatment process: massage and application treatment (with the Chinese medicine ointment obtained in Example 2) for 3 consecutive days.

[0135] Educational guidance: Eat a light diet, avoid cold and greasy foods, avoid allergenic foods and contact with allergens, get enough rest, avoid catching cold, and prevent cross infection.

[0136] Health education effect evaluation: Already aware of follow-up treatment instructions: Follow-up visit after 5 days

[0137] Disposal:

[0138] Treatment [A] Tuina treatment for malnutrition in children / [A] Chinese medicine ointment massage /

[0139] Third visit: 2024.5.10. After the child cooperated with massage, the indigestion improved, the appetite was good, there was no fever, no wheezing, the general condition was good, the sleep was normal, the bowel movements were normal, and the urination was normal.

[0140] Case 2, Zhang * Yue, female, 2 years and 9 months old, date of consultation: 2024.5.10, location of consultation: Beijing Kyoto Children's Hospital.

[0141] Chief complaint: loss of appetite for 2 weeks (first visit).

[0142] Current medical history: loss of appetite for 2 weeks, occasional bad breath, normal bowel movements and sleep, no other discomfort.

[0143] Current medication history: None

[0144] Past medical history: in good health, denies travel history to epidemic areas, denies relevant epidemiological history;

[0145] Physical examination: body temperature 37℃, pain assessment: none, high risk of falls: yes, fall intervention measures: education, family members accompanying.

[0146] Red tip of tongue, greasy coating

[0147] Preliminary diagnosis (or diagnosis): 1. Malnutrition syndrome 2. Indigestion

[0148] Treatment process: massage therapy (with the Chinese medicinal ointment obtained in Example 2) for 3 consecutive days.

[0149] Educational guidance: Provided

[0150] Evaluation of the effectiveness of health education: Knowing.

[0151] Follow-up treatment instructions: If the condition does not improve or new changes occur, please return for follow-up treatment in a timely manner.

[0152] Disposal:

[0153] Treatment [A] Tuina treatment for malnutrition in children / [A] Chinese medicine ointment massage / [A] Acupoint application treatment /

[0154] Second visit: 2024.5.13. After the child cooperated with the massage, his appetite improved, his breath was not obvious, his general condition was good, his sleep was normal, and his urination and defecation were normal.

[0155] Treatment process: massage treatment (with the Chinese medicinal ointment obtained in Example 2), for 3 consecutive days.

[0156] Third visit: May 16, 2024. After cooperating with massage, the child had a good appetite, was in good general condition, had normal sleep, and had normal urination and defecation.

[0157] Case 3, Li * An, male, 1 year and 3 months old, date of consultation: 2024.5.9, location of consultation: Beijing Kyoto Children's Hospital.

[0158] Chief complaint: Difficulty in defecation for 2 days (first visit)

[0159] Current medical history: difficulty in defecation, dry stool, and effortful defecation appeared 2 days ago. Normally, the patient has bowel movements 1-2 times a day. He has no cough, nasal congestion, runny nose, fever, or wheezing. He is in good general condition, has poor diet, normal sleep, and normal urination.

[0160] Current medication history: None

[0161] Past medical history: The patient was in good health, denied any history of infectious diseases or close contact with infectious diseases, denied any history of asthma, and denied any history of foreign body inhalation.

[0162] Physical examination: body temperature 36.5℃, pain assessment: none, high risk of falling: yes, intervention measures for falling: education, accompanied by family members. Conscious, mentally OK, stable breathing, no nasal flaring, no "three-depression sign", no rash, no bleeding spots, superficial lymph nodes not palpable, no conjunctival congestion, no herpes, soft neck, symmetrical and coarse breath sounds in both lungs, no dry or wet rales, strong heart sounds, no murmurs, regular rhythm, soft abdomen, no tenderness, no rebound pain, and warm extremities. Pale red tongue, thin white fur. Light purple fingerprints.

[0163] Preliminary diagnosis (or diagnosis): 1. Dry stool and indigestion 2. Spleen disease (constipation) 3. Spleen deficiency syndrome

[0164] Treatment process: massage, application treatment (with the Chinese medicine ointment obtained in Example 2), on the same day.

[0165] Educational guidance: Eat a light diet, avoid spicy and greasy foods, avoid allergenic foods and contact with allergens, get enough rest, avoid cold, and prevent cross infection.

[0166] Health education effect evaluation: Already aware of follow-up treatment instructions: Return for follow-up in 3 days

[0167] Disposal:

[0168] Treatment [A] Massage therapy for children's constipation / [A] Chinese medicine ointment massage / [A] Acupoint application therapy /

[0169] Second visit: 2020.5.10, follow-up visit for constipation.

[0170] Current medical history: No bowel movement for 3 days. Usually dry stool, labored bowel movement, bowel movement 1-2 times a day, no cough, nasal congestion, runny nose, no fever, no wheezing, generally in good condition, poor diet, normal sleep, normal urination, continue massage treatment.

[0171] Treatment process: massage and application treatment (with the Chinese medicine ointment obtained in Example 2) for 3 consecutive days.

[0172] Third visit: 2020.5.13, defecation. The patient was in good general condition, had a normal diet, normal sleep, dry stool, and normal urination. Massage was continued (with the Chinese medicinal ointment obtained in Example 2) for 3 consecutive days.

[0173] Four examinations: 2020.5.16, general condition is good, normal diet, normal sleep, normal sleep, normal bowel movements, and normal urination.

[0174] The above-mentioned embodiments only express the specific implementation of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A Chinese medicinal ointment for eliminating phlegm, relieving constipation and relieving pain, characterized in that: The invention is prepared from a traditional Chinese medicine liquid and a matrix. The traditional Chinese medicine liquid is prepared from the following raw materials by weight: 90-110 parts of rhubarb, 90-110 parts of scutellaria baicalensis seeds, 90-110 parts of hemp seeds, 90-110 parts of radish seeds, 70-90 parts of cardamom, 90-110 parts of angelica sinensis, and 5-15 parts of cloves. The cardamom and cloves in the traditional Chinese medicine liquid are fermented by a composite bacterial agent; and the composite bacterial strains are Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074.

2. A Chinese medicinal ointment for eliminating phlegm, relieving constipation and relieving pain according to claim 1, characterized in that: The Chinese medicine liquid is prepared from the following raw materials by weight: 100 parts of rhubarb, 100 parts of scutellaria baicalensis seeds, 100 parts of hemp seeds, 100 parts of radish seeds, 80 parts of cardamom seeds, 100 parts of angelica sinensis and 10 parts of cloves.

3. A Chinese medicinal ointment for eliminating phlegm, relieving constipation and relieving pain according to claim 1, characterized in that: The matrix comprises the following raw materials by weight: 10.0-20.0 parts of glyceryl monostearate, 10.0-20.0 parts of paraffin, 50.0-70.0 parts of liquid paraffin, 5.0-7.0 parts of white vaseline, 0.3-0.5 parts of Span 80, 0.5-1.0 parts of OP emulsifier, 0.1-0.3 parts of chlorocresol, and 25.0-30.0 parts of distilled water.

4. A Chinese medicinal ointment for eliminating phlegm, relieving constipation and relieving pain according to claim 3, characterized in that: The matrix comprises the following raw materials by weight: 10.0 parts of glyceryl monostearate, 10.0 parts of paraffin, 50.0 parts of liquid paraffin, 5.0 parts of white vaseline, 0.3 parts of Span 80, 0.5 parts of OP emulsifier, 0.1 parts of chlorocresol, and 25.0 parts of distilled water.

5. A Chinese medicinal ointment for eliminating accumulation, relieving constipation and relieving pain according to claim 1, characterized in that: In the process of preparing the traditional Chinese medicine liquid, rhubarb, scutellaria baicalensis seeds, hemp seeds and radish seeds are mixed, then extracted with water and concentrated to a relative density of 1.1-1.2 (60°C), finally ethanol is used for alcohol precipitation, the supernatant is filtered, and the filtrate is concentrated until there is no alcohol taste.

6. A Chinese medicinal ointment for eliminating phlegm, relieving constipation and relieving pain according to claim 1, characterized in that: In the preparation process of the traditional Chinese medicine liquid, cardamom and clove are fermented by a composite bacterial agent to obtain a fermented product, and then the fermented product and angelica are mixed, extracted with alcohol, filtered to obtain a filtrate, and concentrated to have no alcohol taste.

7. A Chinese medicinal ointment for eliminating phlegm, relieving constipation and relieving pain according to claim 6, characterized in that: The concentration of the Lactobacillus rhamnosus CICC 21006 bacterial solution is 4.8×10 8 ~1.0×10 9 cfu / mL, the concentration of Lactobacillus acidophilus CICC 6074 bacterial solution was 6.5×10 8 ~1.2×10 9 cfu / mL; the amount of Lactobacillus rhamnosus CICC 21006 culture added was 5-10% of the total mass of cardamom and clove, and the amount of Lactobacillus acidophilus CICC 6074 culture added was 5-10% of the total mass of cardamom and clove.

8. A method for preparing a traditional Chinese medicine ointment for eliminating accumulation, relieving constipation and relieving pain according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Material pretreatment: according to the formula, rhubarb, scutellaria baicalensis, hemp seed, radish seed, cardamom, angelica, and clove were weighed and crushed into 80-150 mesh powder respectively; S2, take rhubarb, scutellaria baicalensis, hemp seed, radish seed, add 10 times the amount of water, decoct for 40 minutes, filter, and the filtrate is set aside; the residue, continue to add 8 times the amount of water, decoct for 30 minutes, filter, and the filtrate is set aside; the residue, continue to add 6 times the amount of water, decoct for 30 minutes, filter, discard the residue, the filtrate is combined with the filtrate obtained from the above two times, and concentrated to a relative density of 1.1-1.2 (60°C); put to room temperature, add 95% ethanol to adjust the alcohol content to 70%, seal, maintain the temperature at 16°C, let stand for 48 hours, filter the supernatant, and concentrate the filtrate until there is no alcohol taste to obtain the first concentrated solution for standby; S3, prepared at a concentration of 4.8×10 8 ~1.0×10 9 cfu / mL of Lactobacillus rhamnosus CICC 21006 and a concentration of 6.5×10 8 ~1.2×10 9 cfu / mL of Lactobacillus acidophilus CICC 6074 bacterial solution; S4, taking cardamom and clove, adding Lactobacillus acidophilus CICC 6074 at an inoculum amount of 5-10%, mixing and fermenting in a cylinder, anaerobic fermentation, the temperature is 37 ° C, and the fermentation time is 6-18 hours; then adding Lactobacillus rhamnosus CICC 21006 bacterial solution at an inoculum amount of 5-10% of the total mass of cardamom and clove, aerobic fermentation, the temperature is 37 ° C, the fermentation time is 6-18 hours, and after the fermentation is completed, 3% hydrogen peroxide is added to inactivate Lactobacillus rhamnosus CICC 21006 and Lactobacillus acidophilus CICC 6074 to obtain a fermentation product; S5, take the fermented product obtained in S4 and mix it with Chinese angelica, add 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate it until there is no alcohol taste, to obtain the second concentrated solution for use; S6, mixing the first concentrated solution obtained in S2 and the second concentrated solution obtained in S5 to obtain a Chinese medicine solution; S7. Preparation of matrix: put glyceryl monostearate and paraffin in an evaporating dish, heat and melt in a water bath, then add white vaseline, liquid paraffin and Span 80, and keep the temperature at 80°C until they are completely melted; add OP emulsifier and chlorocresol aqueous solution at the same temperature to the above oil phase solution, stirring continuously in the forward direction while adding, until it solidifies into a milky white semi-solid state; S8. Preparation of ointment film: Mix 4 parts of the matrix obtained in S7 with 1 part of the Chinese medicine solution obtained in S6, add glycerol, adjust the total weight to 1 kg, place in a high-pressure homogenizer, homogenize for 60 minutes under the conditions of flow rate 0.1T / H and pressure 25mPa, and bottle to obtain the Chinese medicine ointment.

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