A kit for detecting porcine epidemic diarrhea virus (PEDV) and its application

Abstract

The present invention discloses a kit for detecting porcine epidemic diarrhea virus (PEDV) and its application, comprising an outer protective shell, wherein a cavity is provided inside the outer protective shell, and the inner wall of the cavity is movably connected to a shaped placement rack, and the top outer wall of the shaped placement rack is provided with a plurality of placement grooves, and the inner walls of the plurality of placement grooves are respectively movably connected with a negative control liquid carrier tube, a positive control liquid carrier tube, an internal reference quality control liquid carrier tube, an enzyme reaction liquid carrier tube and a primer probe liquid carrier tube, and the bottom outer walls of the enzyme reaction liquid carrier tube and the primer probe liquid carrier tube are both provided with an integrated braking connection device. In the present invention, a staff member removes the liquid carrier tube by pulling out the shaped placement rack located in the outer protective shell, and the rack located in the outer protective shell will abut against the main gear, causing the main gear to engage and rotate, so that when the staff member takes it, the liquid in the liquid carrier tube is shaken evenly, which is convenient for the staff member to quickly perform on-site testing.

Description

Technical Field

[0001] The present invention belongs to the technical field of kits, and in particular relates to a kit for detecting porcine epidemic diarrhea virus (PEDV) and an application thereof. Background Art

[0002] Porcine viral diarrhea is one of the major infectious diseases that seriously endangers the pig farming industry. Every year, it causes a large number of piglets to suffer from diarrhea and rapid dehydration and death. The disease is often aggravated by mixed infection of multiple diarrhea viruses, resulting in high mortality and serious economic losses. Staff need to conduct reagent testing.

[0003] For example, a G17 test kit disclosed in the national patent publication number CN216003614U includes a box body, a box cover integrally formed on the top of the box body, a buckle fixedly installed on the bottom of the box cover, a crash pad fixedly installed on the bottom of the box cover, a buckle groove on the top of the box body, a circular groove on the bottom of the inner wall of the box body, a protective device detachably installed inside the circular groove, the protective device includes a protective box, and a mounting plate detachably installed inside the protective box. In the above scheme, the provision of a top corner assembly can support the eight corners inside the test kit to prevent it from being deformed due to collision during transportation, thereby strengthening the strength of the test kit and achieving the effect of improving the ability to protect the internal reagent tubes; the provision of a protective device can further improve the box body's ability to protect the reagent tubes, and the use of cardboard in conjunction with the mounting groove can effectively and firmly place the reagent tubes to prevent them from falling due to shaking during transportation, thereby improving the overall protection of the test kit.

[0004] However, the conventional device still has the following problems when used:

[0005] Currently, the PCR method is commonly used in China to detect PEDV, but this method has high requirements for experimental operation. Existing test kits are difficult to assist in the rapid and accurate detection of PEDV at the sampling site. In addition, the liquid carrier tube of the test kit is relatively light, which can easily lead to the primer probe being placed unstable and spilled. Therefore, it is of great significance to establish a test kit for a convenient and rapid on-site detection method. Summary of the Invention

[0006] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a kit for detecting porcine epidemic diarrhea virus (PEDV) and its application, which has the advantage of being a convenient and rapid on-site detection method for staff.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a kit for detecting porcine epidemic diarrhea virus (PEDV) and its application, comprising an outer protective shell, a cavity being provided inside the outer protective shell, the inner wall of the cavity being movably connected to a shaped placement rack, a top outer wall of the shaped placement rack being provided with a plurality of placement grooves, the inner walls of the plurality of placement grooves being movably connected to a negative control liquid carrier tube, a positive control liquid carrier tube, an internal reference quality control liquid carrier tube, an enzyme reaction liquid carrier tube and a primer probe liquid carrier tube, the bottom outer walls of the enzyme reaction liquid carrier tube and the primer probe liquid carrier tube are both provided with an integrated brake connection device, the cavity is located at the top inner wall position of the integrated brake connection device and is fixedly connected to a rack, and the bottom outer wall of the rack is meshed with the outer wall of the integrated brake connection device.

[0008] Preferably, the integrated brake connection device is provided with three groups, and each group of the integrated brake connection device includes a connecting base plate, a first extension gear, a second extension gear, a third extension gear, a first extension plate, a second extension plate and a third extension plate, the top outer wall of the connecting base plate is movably connected to the bottom outer wall of the primer probe liquid carrying tube, the outer walls at both ends of the first extension plate, the second extension plate and the third extension plate are fixedly connected with spring hinges, the first extension plate, the second extension plate and the third extension plate are hinged to the outer wall of one side of the connecting base plate through the spring hinge, and the outer wall of one side of the multiple spring hinges is connected to the connecting base plate. One side outer wall of the bottom plate is hinged, the first extension plate is hinged to one side outer wall of the first extension gear through a spring hinge, the second extension plate is hinged to one side outer wall of the second extension gear through a spring hinge, and the third extension plate is hinged to one side outer wall of the third extension gear through a spring hinge. One end outer wall of the first extension gear and the second extension gear are both provided with a first groove, and one side outer wall of the third extension plate is movably fitted with one side inner wall of the first groove, and one end outer wall of the first extension gear is provided with a second groove, and one side outer wall of the second extension plate is movably fitted with one side inner wall of the second groove.

[0009] Preferably, the second extension gear and the third extension gear are both provided with a passage groove for the second extension plate and the third extension plate to flip through, and the first extension gear, the second extension gear and the third extension gear can be combined into a main gear by folding the first extension plate, the second extension plate and the third extension plate, and the outer wall of the main gear is meshed and connected with the outer wall of the rack.

[0010] Preferably, the rack, the first stretching gear, the second stretching gear and the third stretching gear are all made of hard and degradable materials.

[0011] Preferably, a slide groove is provided on the top of the outer wall of one side of the connecting bottom plate, a slider is fixedly connected to the bottom outer wall of the primer probe liquid-carrying tube, and the outer wall of the slider is slidably connected to the inner wall of the slide groove.

[0012] Preferably, a cardboard guide rail is fixedly attached to the bottom of the inner wall of the cavity, a guide groove is opened on one side of the bottom outer wall of the shaping placement frame, and the inner wall of the guide groove is slidably connected to the outer wall of the cardboard guide rail.

[0013] Preferably, the inner wall of the placement groove is fixedly connected to a plurality of brackets, the outer wall of the bracket is rotatably connected to a roller, and the outer wall of the primer probe liquid-carrying tube is movably connected to the outer walls of the plurality of rollers.

[0014] Preferably, a stirring tube is fixedly connected to the top inner wall of the tube cap, and the outer wall of the stirring tube at one end away from the tube cap extends to the internal position of the liquid carrying cavity of the primer probe liquid carrying tube through the liquid carrying port of the primer probe liquid carrying tube, and the stirring tube is located at the outer side of the top inner wall of the tube cap.

[0015] Preferably, a threaded screw groove is provided on the top outer wall of the primer probe liquid carrier tube, and a tube cap is provided on the top outer wall of the primer probe liquid carrier tube. The inner wall of the tube cap is threadedly connected to the outer wall of the threaded screw groove, and the top outer wall of the tube cap is fixedly connected to a limiting plug. A slot corresponding to the size of the limiting plug is provided on the inner wall of one side of the placement groove, and the inner wall of the slot is movably connected to the outer wall of the limiting plug.

[0016] Preferably, the inner wall of the placement groove is fixedly connected with an arc-shaped baffle, the curvature of the inner wall of the placement groove is slightly higher than 180°, the arc-shaped baffle is made of plastic material and has a certain resilience toughness, and the outer wall of the primer probe liquid-carrying tube is fixedly connected with a baffle ring, and the bottom outer wall of the baffle ring is movably engaged with the top outer wall of the arc-shaped baffle.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] In the present invention, the staff takes out the liquid-carrying tube by pulling out the shaping placement rack located in the outer protective shell. During the displacement of the shaping placement rack, the rack located in the outer protective shell will abut against the main gear, causing the main gear to engage and rotate. The rotation of the main gear drives the enzyme reaction liquid-carrying tube and the primer probe liquid-carrying tube to rotate, so that the liquid in the liquid-carrying tube is shaken evenly when the staff takes it out, which is convenient for the staff to quickly perform on-site testing. After the staff takes out the enzyme reaction liquid-carrying tube and the primer probe liquid-carrying tube, the main gear is disassembled and extended through the integrated braking connection device. At this time, the first extension gear, the second extension gear and the third extension gear are at the same horizontal plane height, and are evenly distributed in an equilateral triangle shape with the connecting bottom plate as the axis. Through this device, the main gear is transformed into a triangular support frame, which can improve the placement stability of the liquid-carrying tube. When there is no test tube placement rack on site, this device can assist the staff to quickly perform on-site testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0020] Figure 2 Schematic diagram of the internal structure of the outer protective shell of the present invention.

[0021] Figure 3 It is a structural schematic diagram of the shaping and placing rack of the present invention.

[0022] Figure 4 It is a schematic diagram of the bottom structure of the shaping placement rack of the present invention.

[0023] Figure 5 It is a schematic diagram of the placement groove structure of the present invention.

[0024] Figure 6 Schematic diagram of the liquid-carrying tube structure of the present invention.

[0025] Figure 7 Schematic diagram of the pipe cap structure of the present invention.

[0026] Figure 8 It is a schematic diagram of the overall structure of the liquid-carrying tube of the present invention.

[0027] Figure 9 for Figure 8 A magnified schematic diagram of the structure at point A in the middle.

[0028] Figure 10 It is a schematic structural diagram of the main gear of the present invention after it is unfolded.

[0029] Figure 11 It is a schematic structural diagram of the main gear of the present invention after it is unfolded.

[0030] Figure 12 for Figure 11 A magnified schematic diagram of the structure at point B.

[0031] In the figure: 1. Outer protective shell; 2. Shaping rack; 3. Placement slot; 4. Negative control liquid carrier tube; 5. Positive control liquid carrier tube; 6. Internal reference quality control liquid carrier tube; 7. Enzyme reaction liquid carrier tube; 8. Primer probe liquid carrier tube; 9. Rack; 10. Connecting base plate; 11. Spring hinge; 12. First extension gear; 13. Second extension gear; 14. Third extension gear; 15. First groove; 16. Slider; 17. Slide; 18. Tube cap; 19. Threaded screw groove; 20. Stirring tube; 21. Limiting plug; 22. Slot; 23. Arc baffle; 24. Bracket; 25. Roller; 26. Cardboard guide rail; 27. Guide groove; 28. Retaining ring; 29. ​​First extension plate; 30. Second extension plate; 31. Third extension plate; 32. Second groove. DETAILED DESCRIPTION

[0032] In order to clearly and completely describe the objectives and technical solutions of the present invention and make the advantages more clearly understood, the embodiments of the present invention are further described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are part of the embodiments of the present invention, not all of them, and are only used to explain the embodiments of the present invention, not to limit the embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] For example 1, please refer to Figures 1 to 12The present invention provides a technical solution: a kit for detecting porcine epidemic diarrhea virus (PEDV) and its application, comprising an outer protective shell 1, characterized in that: a cavity is provided inside the outer protective shell 1, the inner wall of the cavity is movably connected to a shaping placement rack 2, a top outer wall of the shaping placement rack 2 is provided with a plurality of placement grooves 3, the inner walls of the plurality of placement grooves 3 are respectively movably connected with a negative control liquid carrier tube 4, a positive control liquid carrier tube 5, an internal reference quality control liquid carrier tube 6, an enzyme reaction liquid carrier tube 7 and a primer probe liquid carrier tube 8, the bottom outer walls of the enzyme reaction liquid carrier tube 7 and the primer probe liquid carrier tube 8 are both provided with an integrated brake connection device, and the cavity is located at the top of the integrated brake connection device. A rack 9 is fixedly connected to the inner wall, and the bottom outer wall of the rack 9 is meshed with the outer wall of the integrated brake connecting device. The integrated brake connecting device is provided with three groups, and each group of integrated brake connecting devices includes a connecting bottom plate 10, a first stretching gear 12, a second stretching gear 13, a third stretching gear 14, a first extension plate 29, a second extension plate 30 and a third extension plate 31. The top outer wall of the connecting bottom plate 10 is movably connected to the bottom outer wall of the primer probe liquid carrying tube 8, and the outer walls of both ends of the first extension plate 29, the second extension plate 30 and the third extension plate 31 are fixedly connected with a spring hinge 11. The first extension plate 29, the second extension plate 30 and the third extension plate 31 is hinged to one side outer wall of the connecting base plate 10 through a spring hinge 11, one side outer wall of multiple spring hinges 11 is hinged to one side outer wall of the connecting base plate 10, the first extension plate 29 is hinged to one side outer wall of the first stretching gear 12 through a spring hinge 11, the second extension plate 30 is hinged to one side outer wall of the second stretching gear 13 through a spring hinge 11, the third extension plate 31 is hinged to one side outer wall of the third stretching gear 14 through a spring hinge 11, one end outer wall of the first stretching gear 12 and the second stretching gear 13 are both provided with a first groove 15, one side outer wall of the third extension plate 31 is movably fitted with one side inner wall of the first groove 15, and the first stretching gear 12 and the second stretching gear 13 are respectively provided with a ... A second groove 32 is provided on the outer wall at one end of the gear 12, and an outer wall on one side of the second extension plate 30 is movably fitted with an inner wall on one side of the second groove 32. The second stretching gear 13 and the third stretching gear 14 are both provided with a passage groove for the second extension plate 30 and the third extension plate 31 to flip over and pass through. The first stretching gear 12, the second stretching gear 13 and the third stretching gear 14 can be folded and merged into a main gear by the first extension plate 29, the second extension plate 30 and the third extension plate 31. The outer wall of the main gear is meshed and connected with the outer wall of the rack 9. The rack 9, the first stretching gear 12, the second stretching gear 13 and the third stretching gear 14 are all made of hard and biodegradable materials.

[0034] In the present invention, the staff takes out the liquid-carrying tube by pulling out the shaping mounting rack 2 located in the outer protective shell 1. During the displacement of the shaping mounting rack 2, the rack 9 located in the outer protective shell 1 will abut against the main gear, causing the main gear to engage and rotate. The rotation of the main gear drives the enzyme reaction liquid carrier tube 7 and the primer probe carrier tube 8 to rotate, so that the liquid in the carrier tube is shaken evenly when the staff takes it out, which is convenient for the staff to quickly perform on-site testing. After the staff takes out the enzyme reaction liquid carrier tube 7 and the primer probe carrier tube 8, the main gear is disassembled and extended through the integrated brake connection device. At this time, the first extension gear 12, the second extension gear 13 and the third extension gear 14 are disassembled and extended. The three extending gears 14 are at the same horizontal height and are evenly distributed in an equilateral triangle shape with the connecting base plate 10 as the axis. Through this device, the main gear is transformed into a triangular support frame, which can improve the placement stability of the liquid-carrying tube. When there is no experimental test tube placement rack on site, this device can assist staff to quickly conduct on-site testing. Moreover, this device is made of hard and degradable materials, which is more suitable for high-consumption medical environments and has a low cost. It should be noted that the primer probe liquid-carrying tube 8 in the present invention is loaded with a primer probe group, which is a primer probe designed based on all PEDV domestic strains published by NCBI. In order to overcome the problem of missed detection of old primer probes, at the same time, the detection sensitivity reaches 10 copies / μL.

[0035] In the second embodiment, based on the first embodiment, a slide groove 17 is provided on the top of the outer wall of one side of the connecting bottom plate 10 , a slider 16 is fixedly connected to the bottom outer wall of the primer probe liquid carrying tube 8 , and the outer wall of the slider 16 is slidably connected to the inner wall of the slide groove 17 .

[0036] In the present invention, if the staff needs to place the liquid-carrying tube on a centrifuge or a laboratory clamp after the test, they can press the connecting base plate 10 with their fingers and use the sliding connection of the slide groove 17 and the slider 16 to separate the integrated brake connection device from the liquid-carrying tube, so that the liquid-carrying tube becomes a traditional liquid-carrying test tube, which is convenient for matching with various experimental machines. This device further improves the versatility of the present invention.

[0037] Example 3, based on Example 1, a cardboard guide rail 26 is fixedly attached to the bottom of the inner wall of the cavity, a guide groove 27 is opened on one side of the bottom outer wall of the shaping placement rack 2, the inner wall of the guide groove 27 is slidingly connected to the outer wall of the cardboard guide rail 26, the inner wall of the placement groove 3 is fixedly connected to a plurality of brackets 24, the outer wall of the bracket 24 is rotatably connected to the roller 25, and the outer wall of the primer probe liquid carrier tube 8 is movably connected to the outer wall of the plurality of rollers 25.

[0038] By setting up the roller 25, the friction between the liquid carrying tube and the inner wall of the placement groove 3 is reduced, so that the liquid carrying tube can rotate synchronously with the rotation of the main gear in the placement groove 3. By setting up the cardboard guide slide rail 26 support, the placement groove 3 can be further limited, so that the main gear can be firmly fitted with the rack 9.

[0039] Example 4, on the basis of Example 1, the top inner wall of the tube cap 18 is fixedly connected with a stirring tube 20, the outer wall of the stirring tube 20 away from the tube cap 18 extends to the inner position of the liquid carrying cavity of the primer probe liquid carrying tube 8 through the liquid carrying port of the primer probe liquid carrying tube 8, the stirring tube 20 is located at the outer side of the top inner wall of the tube cap 18, the top outer wall of the primer probe liquid carrying tube 8 is provided with a threaded screw groove 19, the top outer wall of the primer probe liquid carrying tube 8 is provided with a tube cap 18, the inner wall of the tube cap 18 is threadedly connected to the outer wall of the threaded screw groove 19, and the tube cap 1 The top outer wall of 8 is fixedly connected to the limiting plug 21, and a slot 22 corresponding to the size of the limiting plug 21 is opened on the inner wall of one side of the placement groove 3. The inner wall of the slot 22 is movably connected to the outer wall of the limiting plug 21, and the inner wall of the placement groove 3 is fixedly connected to the arc-shaped baffle 23. The curvature of the inner wall of the placement groove 3 is slightly higher than 180°. The arc-shaped baffle 23 is made of plastic material and has a certain resilience toughness. The outer wall of the primer probe liquid carrier tube 8 is fixedly connected to the retaining ring 28, and the bottom outer wall of the retaining ring 28 is movably engaged with the top outer wall of the arc-shaped baffle 23.

[0040] In the present invention, a stirring tube 20 is provided so that the liquid inside the liquid-carrying tube is stirred synchronously when it rotates, and the liquid-carrying tube is limited by the arc-shaped baffle 23 and the baffle ring 28 to prevent the liquid-carrying tube from sliding laterally out of the placement groove 3. Moreover, after the liquid-carrying tube is placed in the placement groove 3, the liquid-carrying tube is pushed up so that the limiting plug 21 enters the slot 22, thereby preventing the liquid-carrying tube from directly falling out. When the limiting plug 21 is located in the slot 22, the length of the liquid-carrying tube plus the main gear is exactly equal to the length of the placement groove 3. After the shaping placement frame 2 is placed in the first extension plate 1, the main gear can just rest against the rack 9, thereby ensuring the structural stability of the present invention.

[0041] The working principle and use process of the present invention: In the present invention, the staff takes out the liquid-carrying tube by pulling out the shaping mounting frame 2 located in the outer protective shell 1. During the displacement of the shaping mounting frame 2, the rack 9 located in the outer protective shell 1 will be against the main gear, causing the main gear to engage and rotate. The rotation of the main gear drives the enzyme reaction liquid carrier tube 7 and the primer probe carrier tube 8 to rotate, so that when the staff takes them out, the liquid in the carrier tube has been shaken evenly, which is convenient for the staff to quickly perform on-site testing. After the staff takes out the enzyme reaction liquid carrier tube 7 and the primer probe carrier tube 8, the main gear is disassembled and extended through the integrated brake connection device. At this time, the first extension gear 12 and the second extension gear 8 are disassembled and extended. The expansion gear 13 and the third expansion gear 14 are at the same horizontal plane height, and are evenly distributed in an equilateral triangle shape with the connecting bottom plate 10 as the axis. Through this device, the main gear is transformed into a triangular support frame, which can improve the placement stability of the liquid-carrying tube. When there is no experimental test tube placement rack on site, this device can assist staff to quickly perform on-site detection. Moreover, this device is made of hard and degradable materials, which is more suitable for high-consumption medical environments and has a low cost. It should be noted that the primer probe liquid-carrying tube 8 in the present invention is loaded with a primer probe group, which is a primer probe designed based on all PEDV domestic strains published by NCBI. In order to overcome the problem of missed detection of old primer probes, at the same time, the detection sensitivity reaches 10 copies / μL. In the present invention, if the subsequent staff needs to place the liquid-carrying tube on a centrifuge or a laboratory clamp, they can press the connecting bottom plate 10 with their fingers and use the sliding connection of the slide groove 17 and the slider 16 to separate the integrated brake connection device from the liquid-carrying tube, so that the liquid-carrying tube becomes a traditional liquid-carrying test tube, which is convenient for matching with various experimental machines. This device further improves the versatility of the present invention. By setting the roller 25 to reduce the friction between the liquid-carrying tube and the inner wall of the placement groove 3, the liquid-carrying tube can rotate synchronously with the rotation of the main gear in the placement groove 3. By setting the cardboard guide slide rail 26 to support it, it can The placement groove 3 is further limited so that the main gear can fit firmly with the rack 9. In the present invention, the liquid carrier tube is limited by the arc baffle 23 and the baffle ring 28 to prevent the liquid carrier tube from sliding out of the placement groove 3 laterally. Moreover, after the liquid carrier tube is placed in the placement groove 3, the liquid carrier tube is pushed up so that the limiting plug 21 enters the slot 22, thereby preventing the liquid carrier tube from directly falling out. When the limiting plug 21 is located in the slot 22, the length of the liquid carrier tube plus the main gear is exactly equal to the length of the placement groove 3. After the shaping placement frame 2 is placed in the first extension plate 1, the main gear can just rest on the rack 9, thereby ensuring the structural stability of the present invention.

[0042] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A kit for detecting porcine epidemic diarrhea virus, comprising an outer protective shell (1), characterized in that: The outer protective shell (1) is provided with a cavity inside, and the inner wall of the cavity is movably connected to a shaping placement rack (2), and the top outer wall of the shaping placement rack (2) is provided with a plurality of placement grooves (3), and the inner walls of the plurality of placement grooves (3) are respectively movably connected to a negative control liquid carrier tube (4), a positive control liquid carrier tube (5), an internal reference quality control liquid carrier tube (6), an enzyme reaction liquid carrier tube (7) and a primer probe liquid carrier tube (8), and the bottom outer walls of the enzyme reaction liquid carrier tube (7) and the primer probe liquid carrier tube (8) are both provided with an integrated braking connection device, and the cavity is fixedly connected to a rack (9) at the top inner wall position of the integrated braking connection device, and the bottom outer wall of the rack (9) is connected to the integrated braking connection device. The outer wall is meshed and connected, and the integrated braking connection device is provided with three groups, and each group of the integrated braking connection device includes a connecting base plate (10), a first stretching gear (12), a second stretching gear (13), a third stretching gear (14), a first extension plate (29), a second extension plate (30) and a third extension plate (31). The top outer wall of the connecting base plate (10) is movably connected to the bottom outer wall of the primer probe liquid carrying tube (8), and the outer walls of both ends of the first extension plate (29), the second extension plate (30) and the third extension plate (31) are fixedly connected with a spring hinge (11). The first extension plate (29), the second extension plate (30) and the third extension plate (31) are connected by the spring hinge ( 11) is hinged to an outer wall of one side of the connecting base plate (10), an outer wall of one side of the plurality of spring hinges (11) is hinged to an outer wall of one side of the connecting base plate (10), the first extension plate (29) is hinged to an outer wall of one side of the first stretching gear (12) through the spring hinge (11), the second extension plate (30) is hinged to an outer wall of one side of the second stretching gear (13) through the spring hinge (11), the third extension plate (31) is hinged to an outer wall of one side of the third stretching gear (14) through the spring hinge (11), one end outer wall of the first stretching gear (12) and the second stretching gear (13) is provided with a first groove (15), one side outer wall of the third extension plate (31) is hinged to the outer wall of the third stretching gear (14), and the first extension gear (12) and the second extension gear (13) are hinged to the outer wall of the third extension plate (31). The inner wall of one side of a groove (15) is movably fitted, the outer wall of one end of the first extension gear (12) is provided with a second groove (32), the outer wall of one side of the second extension plate (30) is movably fitted with the inner wall of one side of the second groove (32), the second extension gear (13) and the third extension gear (14) are both provided with a passage groove for the second extension plate (30) and the third extension plate (31) to flip and pass through, the first extension gear (12), the second extension gear (13) and the third extension gear (14) can be folded and merged into a main gear by the first extension plate (29), the second extension plate (30) and the third extension plate (31), and the outer wall of the main gear is meshed and connected with the outer wall of the rack (9).

2. A kit for detecting porcine epidemic diarrhea virus according to claim 1, characterized in that: The rack (9), the first stretching gear (12), the second stretching gear (13) and the third stretching gear (14) are all made of a hard and degradable material.

3. A kit for detecting porcine epidemic diarrhea virus according to claim 1, characterized in that: A slide groove (17) is provided on the top of the outer wall of one side of the connecting base plate (10), and a slider (16) is fixedly connected to the outer wall of the bottom of the primer probe liquid-carrying tube (8), and the outer wall of the slider (16) is slidably connected to the inner wall of the slide groove (17).

4. A kit for detecting porcine epidemic diarrhea virus according to claim 1, characterized in that: A cardboard guide rail (26) is fixedly attached to the bottom of the inner wall of the cavity, and a guide groove (27) is provided on one side of the bottom outer wall of the shaping placement frame (2), and the inner wall of the guide groove (27) is slidably connected to the outer wall of the cardboard guide rail (26).

5. A kit for detecting porcine epidemic diarrhea virus according to claim 1, characterized in that: The inner wall of the placement groove (3) is fixedly connected to a plurality of brackets (24), the outer wall of the bracket (24) is rotatably connected to a roller (25), and the outer wall of the primer probe liquid-carrying tube (8) is movably connected to the outer walls of the plurality of rollers (25).

6. A kit for detecting porcine epidemic diarrhea virus according to claim 5, characterized in that: The top outer wall of the primer probe liquid carrying tube (8) is provided with a threaded screw groove (19), and the top outer wall of the primer probe liquid carrying tube (8) is provided with a tube cap (18), the inner wall of the tube cap (18) is threadedly connected to the outer wall of the threaded screw groove (19), the top outer wall of the tube cap (18) is fixedly connected to the limiting plug (21), and the inner wall of one side of the placement groove (3) is provided with a slot (22) corresponding to the size of the limiting plug (21), and the inner wall of the slot (22) is movably connected to the outer wall of the limiting plug (21).

7. A kit for detecting porcine epidemic diarrhea virus according to claim 6, characterized in that: The top inner wall of the tube cap (18) is fixedly connected with a stirring tube (20), and the outer wall of one end of the stirring tube (20) away from the tube cap (18) extends to the inner position of the liquid carrying cavity of the primer probe liquid carrying tube (8) through the liquid carrying port of the primer probe liquid carrying tube (8). The stirring tube (20) is located at the outer side of the top inner wall of the tube cap (18). The inner wall of the placement groove (3) is fixedly connected with an arc-shaped baffle (23). The curvature of the inner wall of the placement groove (3) is slightly higher than 180°. The arc-shaped baffle (23) is made of plastic material and has a certain resilience toughness. The outer wall of the primer probe liquid carrying tube (8) is fixedly connected with a retaining ring (28), and the bottom outer wall of the retaining ring (28) is movably engaged with the top outer wall of the arc-shaped baffle (23).

8. A use of a kit for detecting porcine epidemic diarrhea virus, characterized in that: A kit for detecting porcine epidemic diarrhea virus comprising the kit according to any one of claims 1 to 7, wherein the kit comprises the following steps:

1. The staff takes out the liquid-carrying tube by pulling out the shaping mounting frame (2) located in the outer protective shell (1). During the displacement of the shaping mounting frame (2), the rack (9) located in the outer protective shell (1) will abut against the main gear, causing the main gear to engage and rotate. The rotation of the main gear drives the enzyme reaction liquid-carrying tube (7) and the primer probe liquid-carrying tube (8) to rotate, so that the liquid in the liquid-carrying tube is shaken evenly when the staff takes it out, which facilitates the staff to quickly perform on-site testing; 2. After the staff takes out the enzyme reaction liquid carrier tube (7) and the primer probe carrier tube (8), the main gear is disassembled and stretched through the integrated brake connection device. At this time, the first stretching gear (12), the second stretching gear (13) and the third stretching gear (14) are at the same horizontal plane height, and are evenly distributed in an equilateral triangle shape with the connecting bottom plate (10) as the axis. Through this device, the main gear is transformed into a triangular support frame, which can improve the placement stability of the liquid carrier tube. When there is no experimental test tube placement rack on site, this device can assist the staff to quickly perform on-site testing.

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