Amino acid sunscreen lotion for repairing fish scale collagen and preparation method thereof

Abstract

The invention discloses an amino acid sunscreen lotion for repairing fish scale collagen and a preparation method thereof. The sunscreen lotion comprises the following components in parts by weight: 18-25 parts of a sunscreen, 3.5-4.5 parts of an emulsifier and stabilizer, 6.5-8.5 parts of a skin feel regulator, 3-6 parts of a moisturizer, 4.2-6.2 parts of an emollient, 0.01-0.05 parts of a fragrance, 3-5 parts of an effective ingredient, and the balance of purified water. The present invention rationally compounds the components based on their properties to ensure a suitable skin feel and maintain a reasonable product form. In the sunscreen, a variety of fat-soluble sunscreen ingredients are selected to compound a water-based sunscreen agent, methylene bis-benzotriazolyl tetramethylbutylphenol and deoxynyl glucoside mixture, to expand the ultraviolet absorption wavelength range, and provide a higher sunscreen base by controlling the appropriate mass fraction; light fat components such as calcium starch octenylsuccinate, polymethylsilsesquioxane, and C9-12 alkane are selected to reduce the sticky feeling. The sunscreen lotion provided by the present invention is mild and comfortable, moisturizing and clear, non-sticky and non-whitening, has good film-forming properties, and excellent moisturizing performance.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to an amino acid sunscreen lotion for repairing fish scale collagen and a preparation method thereof. Background Art

[0002] Ultraviolet radiation can damage human skin. Short-term exposure can cause erythema or sunburn, while long-term exposure can lead to premature aging and skin cancer. Sunscreen is a key way to protect against UV radiation. Currently, sunscreen products on the market primarily fall into two categories: chemical and physical. Chemical sunscreens, such as oxybenzone, absorb UV rays using chemicals that convert them into heat and dissipate it. These chemicals effectively absorb UV rays, providing comprehensive protection. Their lightweight texture makes them easy to apply and absorb, making them suitable for daily use. However, some ingredients may cause skin irritation or even allergic reactions. Physical sunscreens use substances such as zinc oxide and titanium dioxide to reflect and scatter UV rays, creating a barrier effect. These ingredients generally do not cause allergic reactions and form a protective layer on the skin, reflecting and scattering UV rays to protect the skin. However, physical sunscreens can be heavy, appearing fake white, and appearing greasy, making them unsuitable for oily skin or those who prefer a light, refreshing feel.

[0003] Patent CN117159398B discloses a superfluid composite sunscreen and oil-in-water-in-oil triple sunscreen lotion. The sunscreen composition comprises 0.1% to 60% of the sunscreen ingredients, including ethylhexyl salicylate, ethylhexyl triazone, diethylamino hydroxybenzoyl hexyl benzoate, and octocrylene. While the lotion is thin, easy to apply, refreshing and moisturizing, and has good storage stability, it contains a high concentration of chemical sunscreens, making it difficult to avoid potential skin damage.

[0004] Therefore, it is of great significance to prepare an oil-in-water sunscreen with high SPF and PA values, mild and comfortable, moisturizing and clear characteristics, and at the same time with the effects of repairing, mild and non-irritating. Summary of the Invention

[0005] The purpose of the present invention is to address the shortcomings and deficiencies of the above existing technologies and provide an amino acid sunscreen lotion for repairing fish scale collagen and a preparation method thereof, thereby solving the following technical problems:

[0006] Pure chemical sunscreen system products have poor photostability and poor sun protection effect. In addition, sunscreen agents are easily absorbed by the skin, causing irritation to the skin and low safety.

[0007] To achieve the first object of the present invention, the present invention adopts the following technical solutions:

[0008] The invention discloses an amino acid sunscreen lotion for repairing fish scale collagen. The sunscreen lotion comprises the following components in parts by weight: 18-25 parts of a sunscreen, 3.5-4.5 parts of an emulsifier and stabilizer, 6.5-8.5 parts of a skin feel regulator, 3-6 parts of a moisturizer, 4.2-6.2 parts of an emollient, 0.01-0.05 parts of a fragrance, 3-5 parts of an effective ingredient, and the balance being purified water.

[0009] As a further improvement, the sunscreen agent of the present invention is composed of one or more of a methylene bis-benzotriazolyl tetramethylbutylphenol mixture, diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl methoxycinnamate, ethylhexyl triazone, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl salicylate, and titanium dioxide.

[0010] As a further improvement, the emulsifier and stabilizer described in the present invention is composed of one or more of arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 glyceryl stearate.

[0011] As a further improvement, the skin feel regulator of the present invention is composed of one or more of calcium starch octenylsuccinate, polymethylsilsesquioxane, C9-12 alkane, cyclopentasiloxane, and isododecane.

[0012] As a further improvement, the moisturizing agent of the present invention is composed of one or more of butylene glycol, glyceryl polyether-26, ethylene glycol, propylene glycol, panthenol, and sodium hyaluronate.

[0013] As a further improvement, the effective ingredients of the present invention are composed of one or more of fish scale collagen, carboxymethyl chitosan, mycosporin-like amino acids, and dipotassium glycyrrhizate.

[0014] As a further improvement, the emollient of the present invention comprises one or more of cetyl dimethicone, NaturalBio HB, sorbitol, mannitol, and polyethylene glycol.

[0015] The present invention also discloses a method for preparing a fish scale collagen repair amino acid sunscreen lotion, comprising the following steps:

[0016] S1: Weigh the phase A material in an emulsifying tank and stir until completely dissolved. The stirring power is 10Hz-30Hz and the stirring temperature is 75℃-85℃;

[0017] S2: Weigh the phase B material and add it to the batching tank, turn on the batching tank heating system, and stir at 75℃-85℃ until the material is completely dissolved;

[0018] S3: Add the phase B material in the batching tank to the emulsification tank. After the materials are added, homogenize at 40Hz-55Hz for 20min-90min;

[0019] S4: After homogenization, cool to below 50°C with stirring, weigh the phase C material and add it to the emulsification tank, homogenize at 40Hz-55Hz for 10min-30min;

[0020] S5: Weigh the phase D material, dissolve it evenly, add it to the emulsification tank, stir it for 10-30 minutes, let it stand after stirring, and fill it after the prepared liquid is defoamed and stabilized to obtain the sunscreen lotion;

[0021] Phase A contains sunscreen, moisturizer, active ingredients, and purified water, including:

[0022] The sunscreen in phase A is a mixture of methylene bis-benzotriazolyl tetramethylbutylphenol and deoxynyl glucoside;

[0023] The moisturizer in phase A is a combination of one or more of glycereth-26, butylene glycol, ethylene glycol, propylene glycol, panthenol, and sodium hyaluronate;

[0024] The active ingredient in phase A is a combination of one or more of dipotassium glycyrrhizate and carboxymethyl chitosan;

[0025] Phase B contains sunscreen, emulsifier and stabilizer, skin feel regulator, emollient, among which:

[0026] The sunscreen in phase B is a combination of one or more of ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl triazone, ethylhexyl salicylate, and titanium dioxide;

[0027] The emulsifier and stabilizer in phase B is a combination of one or more of arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 glyceryl stearate;

[0028] The skin feel regulator in phase B is a combination of one or more of polymethylsilsesquioxane, C9-12 alkane, cyclopentasiloxane, and isododecane;

[0029] The emollient in phase B is cetyl dimethicone;

[0030] Phase C contains skin feel regulators and emollients, including:

[0031] The skin feel regulator in phase C is a combination of one or more of calcium starch octenylsuccinate and isododecane;

[0032] The emollient in phase C is a combination of one or more of Natural Bio HB sorbitol, mannitol, and polyethylene glycol;

[0033] Phase D contains active ingredients, fragrance, and purified water, including:

[0034] The active ingredients in phase D are a combination of one or more of fish scale collagen and mycosporine-like amino acids;

[0035] The fragrance in phase D is a perfume.

[0036] The present invention utilizes marine biological materials such as fish scale collagen from fish, mycosporine-like amino acids from algae, and carboxymethyl chitosan from shrimp and crab as core ingredients of the sunscreen lotion. The fish scale collagen has a repairing effect, and is compounded with dipotassium glycyrrhizate to effectively prevent potential skin irritation and sensitization of the product, repair the skin, and improve the safety of the product. Carboxymethyl chitosan has good hygroscopicity, antibacterial properties, moisturizing and film-forming properties, and can effectively improve the skin feel of the product and reduce the sticky feeling. Mycosporine-like amino acids, as natural sunscreen ingredients derived from marine algae, can enhance the anti-ultraviolet ability and light stability of the sunscreen lotion after being compounded with chemical sunscreens.

[0037] The protein content in fish scales is between 52% and 78%, mainly collagen and fish scale keratin. Fish scale collagen is mainly type I collagen, which has the functions of enhancing cell activity, promoting cell growth, and making the skin firm and elastic. In cosmetic applications, it has good film-forming properties, strong moisturizing properties, and skin repair effects. Mycosporine-like amino acids (MAAs) are a class of secondary metabolites of aquatic organisms, widely present in aquatic organisms such as algae, phytoplankton, cyanobacteria, marine microorganisms and marine animals. They have biological activities such as ultraviolet light protection, antioxidant, and anti-aging effects. Mycosporine-like amino acids have a high molecular absorption coefficient (12400-58800 L / (mol·cm)) and are powerful UVA absorbing compounds in nature. They can also effectively resist UVB radiation and have a photoprotective effect.

[0038] In summary, compared with the prior art, the present invention has the following beneficial effects:

[0039] 1. Mycosporine-like amino acids are natural biological sunscreens and photostabilizers. By synergizing the effect of mycosporine-like amino acids with other chemical sunscreens at appropriate concentrations and combining them with the adsorption effect of carboxymethyl chitosan, the amount of chemical sunscreen added can be reduced, the product's UV resistance and photostability can be improved, and the problems of low sun protection value and poor photostability of pure chemical sunscreen systems can be solved. The sunscreen lotion prepared by this technology has a sun protection value of SPF50+ and PA++++.

[0040] 2. By adding fish scale collagen, carboxymethyl chitosan, dipotassium glycyrrhizate and other effective ingredients, the potential irritation of the chemical sunscreen system to the skin is neutralized, the safety of product use is improved, and the product has the effects of repairing, being mild and non-irritating.

[0041] 3. The present invention rationally compounds the components based on their properties to ensure a suitable skin feel and maintain a reasonable product form. The sunscreen agent uses a variety of fat-soluble sunscreen ingredients to compound a water-based sunscreen agent methylene bis-benzotriazolyl tetramethylbutylphenol and deoxynyl glucoside mixture to expand the ultraviolet absorption wavelength range, and provides a higher sunscreen base by controlling the appropriate mass fraction; light fat components such as calcium starch octenylsuccinate, polymethylsilsesquioxane, and C9-12 alkane are used to reduce the sticky feeling; oil-in-water emulsifiers and stabilizers are used to maintain the emulsion state, and moisturizers, emollients, etc. are used to further adjust the skin feel of the emulsion; the sunscreen lotion provided by the present invention is mild and comfortable, moisturizing and clear, non-sticky and non-whitening, has good film-forming properties, and excellent moisturizing performance.

[0042] 4. The sunscreen lotion provided by the present invention separates the main components into phases A and B according to their hydrophilic and lipophilic properties and emulsifies them at 75°C-85°C, then cools the temperature to below 50°C and cold-mixes phases C and D, retaining the activity of the components to the greatest extent. The preparation process is simple, highly operational, and has low process costs. By controlling key parameters such as appropriate temperature, stirring speed, and emulsification time, the emulsification effect is ensured and the stability of the emulsion is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Attachment Figure 1 It is a preparation flow chart of the present invention. DETAILED DESCRIPTION

[0044] In order to better illustrate the purpose, technical solutions and features of the present invention, the present invention is further explained below in conjunction with the accompanying drawings and specific embodiments, but the embodiments do not limit the scope of protection of the present invention.

[0045] Example 1

[0046] An amino acid sunscreen lotion for repairing fish scale collagen, characterized in that the sunscreen lotion comprises the following components in parts by mass: 21 parts of sunscreen, 3.6 parts of emulsifier and stabilizer, 7.0 parts of skin feel regulator, 5.0 parts of moisturizer, 5.0 parts of emollient, 0.03 parts of fragrance, 3.8 parts of effective ingredients, and the balance of purified water.

[0047] The sunscreen comprises a methylene bis-benzotriazolyl tetramethylbutylphenol mixture, diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl methoxycinnamate, ethylhexyl triazone, and bis-ethylhexyloxyphenol methoxyphenyl triazine in a mass ratio of 2.5:4.7:7.8:3.5:2.5; the methylene bis-benzotriazolyl tetramethylbutylphenol mixture comprises methylene bis-benzotriazolyl tetramethylbutylphenol, decyl glucoside, xanthan gum, butylene glycol, and water in a mass ratio of 80:17:2.4:0.4:0.2.

[0048] The emulsifier and stabilizer consists of arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol and cetearyl glucoside in a mass ratio of 1.6:0.8:0.4:0.64:0.16.

[0049] The skin feel regulator is composed of calcium starch octenylsuccinate, polymethylsilsesquioxane, and C9-12 alkane in a mass ratio of 1.7:2.3:3.0.

[0050] The moisturizing agent is composed of butylene glycol and glycerol polyether-26 in a mass ratio of 3.5:1.5.

[0051] The effective ingredients are composed of dipotassium glycyrrhizate, fish scale collagen, carboxymethyl chitosan and mycosporin-like amino acids in a mass ratio of 0.15:3.0:0.15:0.5.

[0052] The emollient includes cetyl dimethicone and Natural Bio HB in a mass ratio of 7:3; Natural Bio HB is composed of butylene glycol, p-hydroxyacetophenone, 1,2-hexanediol, agrimony extract, European dryopteris root extract, and white osmanthus extract in a mass ratio of 4.7:2.5:2.5:0.1:0.1:0.1.

[0053] Table 1: Formula of this embodiment

[0054]

[0055] The preparation of the above-mentioned amino acid sunscreen lotion for repairing fish scale collagen comprises the following steps:

[0056] S1: Weigh the phase A material and stir it in an emulsifying tank until it is completely dissolved. The stirring power is 10Hz-30Hz and the stirring temperature is 75℃-85℃.

[0057] S2: Weigh the phase B material and add it to the batching tank. Turn on the heating system of the batching tank and stir at 75℃-85℃ until the material is completely dissolved.

[0058] S3: Add the phase B material in the batching tank to the emulsifying tank. After the material is added, homogenize at 40Hz-55Hz for 20min-90min.

[0059] S4: After homogenization, cool to below 50°C with stirring, weigh the phase C material and add it to the emulsification tank, and homogenize at 40Hz-55Hz for 10min-30min.

[0060] S5: Weigh the Phase D material, dissolve it evenly, and add it to the emulsifier tank. Stir for 10-30 minutes. After stirring, let it stand. After the prepared solution is defoamed and stabilized, it can be filled to obtain the sunscreen lotion.

[0061] The A phase contains sunscreen, moisturizer, functional ingredients, purified water, specifically purified water, dipotassium glycyrrhizate, glyceryl polyether-26, carboxymethyl chitosan, butylene glycol, methylene bis-benzotriazolyl tetramethylbutylphenol and decidyl glucoside mixture; the B phase contains sunscreen, emulsifier and stabilizer, skin feel regulator, emollient, specifically ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl triazone, arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol, cetearyl glucoside, polymethylsilsesquioxane, C9-12 alkane, cetyl polydimethylsiloxane; the C phase contains skin feel regulator, emollient, specifically calcium starch octenylsuccinate, Natural Bio HB; the D phase comprises active ingredients, fragrances, and purified water, specifically camellia fragrance, purified water, fish scale collagen, and mycosporin-like amino acids.

[0062] Comparative Example 1

[0063] An amino acid sunscreen lotion for repairing fish scale collagen and a preparation method thereof adopt the formula and preparation method of Example 1, except that: the mycosporin amino acid in the formula component is replaced by purified water.

[0064] Comparative Example 2

[0065] An amino acid sunscreen lotion for repairing fish scale collagen and a preparation method thereof adopt the formula and preparation method of Example 1, except that the fish scale collagen in the formula is replaced by purified water.

[0066] The sample prepared in Example 1 was tested for physicochemical properties, safety, and efficacy, and the samples prepared in Comparative Examples 1 and 2 were tested for comparative efficacy.

[0067] 1. Physical and chemical testing

[0068] (1) Materials and methods

[0069] According to the "Technical Specifications for Safety of Cosmetics" (2015 edition), the samples were tested for physical and chemical items, including the content of mercury, lead, arsenic, cadmium, dioxane, ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, diethylaminohydroxybenzoyl hexyl benzoate, ethylhexyl triazone, methylene bis-benzotriazolyl tetramethylbutylphenol, and pH value.

[0070] (2) Experimental results

[0071] Table 1: Statistics of physical and chemical index test results

[0072]

[0073] (3) Experimental conclusion

[0074] The contents of mercury, lead, arsenic, cadmium, dioxane, ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, diethylaminohydroxybenzoyl hexyl benzoate, ethylhexyl triazone, and methylene bis-benzotriazolyl tetramethylbutylphenol meet the requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition), and the pH value is 6.3.

[0075] 2. Microbiological Testing

[0076] (1) Materials and methods

[0077] The samples were tested for microorganisms according to the Technical Specifications for Safety of Cosmetics (2015 edition). The test items included total colony count, total mold and yeast count, heat-resistant coliform group, Staphylococcus aureus, and Pseudomonas aeruginosa.

[0078] (2) Experimental results

[0079] Table 2: Statistics of microbiological test results

[0080]

[0081]

[0082] (3) Experimental conclusion

[0083] The total colony count, total mold and yeast count, heat-resistant coliform group, Staphylococcus aureus and Pseudomonas aeruginosa meet the requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0084] 3. Multiple skin irritation tests

[0085] (1) Materials and methods

[0086] According to the "Technical Specifications for Safety of Cosmetics" (2015 edition), the samples were subjected to a skin irritation test. After the experimental animals adapted to the experimental environment for 7 days, the hair of about 3cm×3cm on both sides of the spine on the back was shaved. Subsequently, 0.5mL of the test substance was applied to the left skin every day (area of ​​about 2.5cm×2.5cm), and the right side was used as a control. Apply once a day for 14 consecutive days. From the second day on, use Missha Blackhead Remover Oil to remove residues and cut off new hair before application, and the control side is treated in the same way. Evaluate the skin reaction after 1 hour and score according to Table 1 "Skin Irritation Reaction Score" in Chapter 6 "Skin Irritation / Corrosion Test" of the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0087] (2) Experimental results

[0088] Table 3: Statistical table of the results of multiple skin irritation tests on rabbits

[0089]

[0090]

[0091] Note: The mean integral value is rounded to 2 decimal places.

[0092] (3) Experimental conclusion

[0093] The multiple skin irritation of the test substance to rabbits was: non-irritating.

[0094] 4. Skin allergy test

[0095] (1) Materials and methods

[0096] According to the "Technical Specifications for Safety of Cosmetics" (2015 edition), the samples were subjected to skin allergy test. The experimental animals were first adapted to the experimental environment for 7 days. Induced exposure: 24 hours before the experiment, the hair on the left side of the guinea pig's back was shaved about 4 cm. 2 -6cm 2 , apply 0.2 mL of the test substance, cover with gauze and cellophane, and secure with non-irritating tape for 6 hours. Repeat this procedure on the 7th and 14th days. Use purified water as a negative control, and 1.0% 2,4-dinitrochlorobenzene as a positive control. Provocative exposure: 14 days after induction, shave a 2 cm x 2 cm area on the right side of the face, apply the test substance, and follow the same procedures as above. Use purified water as a negative control, and 0.5% 2,4-dinitrochlorobenzene as a positive control. Observe the reaction 24 and 48 hours after provocation and score according to Table 1, "Allergy Test Skin Reaction Scoring," in Chapter 6, "Skin Allergy Test," of the "Safety Technical Specifications for Cosmetics" (2015 edition). A skin reaction score ≥ 2 is considered positive for skin allergy. The sensitization intensity of the test substance is determined according to Table 3, "Sensitization Intensity."

[0097] (2) Experimental results

[0098] Table 4: Statistical table of the results of the test substance's skin allergy test on guinea pigs (BT method)

[0099]

[0100]

[0101] Note: ① The starting weight and ending weight are expressed as mean ± SD

[0102] ② In the skin reaction intensity column, fill in the ratio of the number of animals that reacted to the number of animals tested when the skin reaction score is 0, 1, 2, 3, etc.

[0103] (3) Experimental conclusion

[0104] The results of the guinea pig skin allergy test of the test substance were: no skin allergic reaction was observed.

[0105] 5. Human skin patch test

[0106] (1) Materials and methods

[0107] Select qualified patch test equipment and use the closed patch test method. Place approximately 0.020g to 0.025g of the test substance in the patch tester and apply it to the subject's back with hypoallergenic tape. Remove the test substance after 24 hours and observe the skin reaction 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0108] (2) Experimental results

[0109] Table 5: Statistics of cosmetics human skin patch test results

[0110]

[0111]

[0112] (3) Experimental conclusion

[0113] The results of human skin patch tests showed that no adverse skin reactions occurred among 30 people.

[0114] 6. Determination of SPF value of sunscreen cosmetics

[0115] (1) Materials and methods

[0116] 1. Test method: Test in accordance with the specific requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). The subject lies prone and receives ultraviolet radiation on the back. The minimum erythema dose (MED value) of the skin to ultraviolet radiation is measured 24 hours before the test, and the irradiation dose is adjusted accordingly. During the test, select a spot no less than 30cm from the subject's back. 2 Area, according to (2.00±0.05) mg / cm 2 Evenly apply the test object or control substance, then irradiate according to the specifications without application, with the control substance, and with the test object. After 24 hours, observe and record the MED values ​​of the three conditions.

[0117] 2. SPF value calculation method: The SPF value of the test article or reference article for protecting a single subject is expressed as follows:

[0118]

[0119] Individual SPF values ​​must be accurate to one decimal place. Calculate the arithmetic mean of all subject SPF values, and take the integer portion as the sample SPF value. When estimating the sampling error of the mean, calculate the standard deviation and standard error of the data. The 95% confidence interval (95% CI) must not exceed 17% of the mean. If this is not possible, add more subjects (up to a maximum of 25) to meet this requirement.

[0120] (2) Experimental results

[0121] Table 6: Statistics of cosmetic SPF test results

[0122] Example 1 Comparative Example 1 Comparative Example 2 SPF value 61 47 58

[0123] 7. Determination of UVA protection index (PFA value) of sunscreen cosmetics

[0124] (1) Materials and methods

[0125] 1. Test method: Test in accordance with the specific requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). The subject lies prone and receives ultraviolet radiation on the back. The minimum darkening amount (MPPD value) of the skin to ultraviolet radiation is predicted 24 hours before the test, and the exposure is adjusted accordingly. 2 Area, evenly apply (2.00±0.05)mg / cm 2 The test object or reference substance was then exposed to UVA at a specific dose according to the specifications. The MPPD values ​​of the three conditions were observed and recorded after 2 to 4 hours.

[0126] 2. PFA value calculation method: The PFA value of the test substance or reference substance protecting a single subject is expressed as follows:

[0127]

[0128] Individual PFA values ​​were calculated to one decimal place. The arithmetic mean of all subjects' PFA values ​​was calculated, and the integer portion was taken as the sample PFA value. The sampling error of the mean was estimated by calculating the standard deviation and standard error. The 95% confidence interval (95% CI) was required to be no more than 17% of the mean. If this was not met, the number of subjects was increased (up to 25) until the requirement was met.

[0129] (2) Experimental results

[0130] Table 7: Statistics of cosmetics PFA value test results

[0131] Example 1 Comparative Example 1 Comparative Example 2 PFA value 20 15 19

[0132] 8. Evaluation of human efficacy

[0133] (1) Test method

[0134] 1. Test / Evaluation Parameters

[0135] Before using the product (DO), 28 days after using the product (D28).

[0136] Table 8: Test parameter evaluation table

[0137]

[0138]

[0139] 2. Testing Process

[0140] Before screening (D0): On the morning of the test, participants were required to keep their faces clean and refrain from cleansing, skincare, or makeup. After signing the consent form, they were asked to sit quietly in a holding area maintained at a constant temperature and humidity (21±1°C, (50±10)% RH) for 20 minutes. Testers screened participants for eligibility, requiring a transepidermal water loss (TEWL) greater than 20g / m² / h. The stratum corneum moisture content and hemoglobin index (EI) were also collected. Product instructions were then explained, and test products and a usage diary were distributed.

[0141] After 28 days of use (D28): On the morning of the test, participants were asked to keep their faces clean. After sitting quietly in a temperature-controlled and humidity-controlled waiting area, testers collected stratum corneum moisture content, TEWL, and EI from the cheek area. Participants were then asked to complete a self-assessment questionnaire (D28).

[0142] (2) Data Analysis

[0143] Statistical analysis software was used for data analysis. Measurement data were expressed as mean ± standard deviation and tested for normal distribution. Before-after comparisons were performed using a paired t-test; otherwise, a two-sample rank sum test was used. For before-after comparisons of rank data, a two-sample rank sum test was used. Comparisons between experimental and control groups were performed using an independent-sample t-test or a rank sum test. All statistical analyses were two-tailed, with a significance level of α = 0.05.

[0144] Significance mark: “ns” means p ≥ 0.05, no significant difference; “*” means 0.01 ≤ p ≤ 0.05, there is a significant difference; “**” means p ≤ 0.01, there is an extremely significant difference.

[0145] (3) Test results

[0146] 1. Skin stratum corneum moisture content (Corneometer CM 825)

[0147] Table 9: Descriptive statistics of moisture content of stratum corneum in cheek area (n=32)

[0148]

[0149]

[0150] Note: Change rate = (measured value after use - measured value before use) / measured value before use * 100%. The higher the value, the higher the moisture content of the skin's stratum corneum.

[0151] 2. Transepidermal Water Loss (TEWL) (Tewameter™ Hex)

[0152] Table 10: Descriptive statistics of transepidermal water loss (TEWL) in the cheek area (n=32)

[0153]

[0154] Note: Change rate = (measured value after use - measured value before use) / measured value before use * 100%. The lower the value, the less transepidermal water loss and the better the skin barrier.

[0155] 3. Skin hemoglobin index El (Mexameter MX 18)

[0156] Table 11: Descriptive statistics of the hemoglobin index (EI) of the cheek area (n=32)

[0157]

[0158] Note: Change rate = (measured value after use - measured value before use) / measured value before use * 100%. The lower the value, the lower the skin hemoglobin index.

[0159] 4. Subject Self-Assessment

[0160] Table 12: Statistics of self-assessment results of subjects (28 days after using the product) (n=32)

[0161]

[0162]

[0163] Scoring criteria: 1. "Agree" = 5 points; "Somewhat agree" = 4 points; "Fair" = 3 points; "Somewhat disagree" = 2 points; "Disagree" = 1 point. 2. The percentage of subjects who scored 24 points or more represents the percentage of subjects who were satisfied.

[0164] (IV) Test conclusion

[0165] Twenty-eight days after using the product in Example 1, the moisture content of the stratum corneum in the cheek area significantly increased by 12.86%. Twenty-eight days after using the product in Example 1, the transepidermal water loss (TEWL) in the cheek area significantly decreased by 15.73%. Twenty-eight days after using the product, the hemoglobin index (EI) in the cheek area significantly decreased by 6.09%. Twenty-eight days after using the product, 100% of the subjects felt their skin was no longer dry or tight.

[0166] 100% of the subjects felt that their skin was more hydrated;

[0167] 97% of the subjects felt that their skin redness had improved;

[0168] 100% of the subjects felt that their skin tolerance improved;

[0169] 100% of the subjects felt that their facial skin was healthier and more stable;

[0170] 100% of the test subjects found the product mild and non-irritating.

[0171] 32 qualified subjects used the test product continuously for 28 days under normal conditions. The skin stratum corneum moisture content, transepidermal water loss rate TEWL, and skin hemoglobin index EI were measured by instruments. Combined with the subjects' self-evaluation, the results showed that the test product in Example 1, "Xinkeyan Hydrating and Clear Sunscreen Milk", has repairing, mild and non-irritating effects.

[0172] All of the above specific embodiments are only part of the implementation plans of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field within the technical scope disclosed by the present invention, and any equivalent replacement or change made by those skilled in the art based on the technical solutions and inventive concepts of the present invention should be included in the protection scope of the present invention.

Claims

1. An amino acid sunscreen lotion for fish scale collagen repair, characterized in that: The sunscreen lotion comprises the following components in parts by weight: 18-25 parts of sunscreen, 3.5-4.5 parts of emulsifier and stabilizer, 6.5-8.5 parts of skin feel regulator, 3-6 parts of moisturizer, 4.2-6.2 parts of emollient, 0.01-0.05 parts of fragrance, 3-5 parts of functional ingredients, and the balance of purified water; the sunscreen consists of methylene bis-benzotriazolyl tetramethylbutylphenol, diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl methoxycinnamate, ethylhexyl triazone, and bis-ethylhexyloxyphenol methoxyphenyl triazine; and the functional ingredients consist of fish scale collagen, carboxymethyl chitin, and mycosporin-like amino acids.

2. The sunscreen lotion according to claim 1, characterized in that The emulsifier and stabilizer is composed of one or more of arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 glyceryl stearate.

3. The sunscreen lotion according to claim 1, characterized in that The skin feel regulator is composed of one or more of calcium starch octenylsuccinate, polymethylsilsesquioxane, C9-12 alkane, cyclopentasiloxane, and isododecane.

4. The sunscreen lotion according to claim 1, 2 or 3, characterized in that: The moisturizing agent is composed of one or more of butylene glycol, glyceryl polyether-26, ethylene glycol, propylene glycol, panthenol, and sodium hyaluronate.

5. The sunscreen lotion according to claim 4, characterized in that The emollient comprises one or more of cetyl dimethicone, Natural Bio HB, sorbitol, mannitol, and polyethylene glycol.

6. A method for preparing the fish scale collagen repair amino acid sunscreen lotion according to claim 1, 2, 3 or 5, characterized in that: The following steps are involved: S1: Weigh the phase A material in an emulsifying tank and stir until completely dissolved. The stirring frequency is 10Hz-30Hz and the stirring temperature is 75℃-85℃; S2: Weigh the phase B material and add it to the batching tank, turn on the batching tank heating system, and stir at 75℃-85℃ until the material is completely dissolved; S3: Add the phase B material in the batching tank to the emulsification tank. After the materials are added, homogenize at 40Hz-55Hz for 20min-90min; S4: After homogenization, cool to below 50°C with stirring, weigh the phase C material and add it to the emulsification tank, homogenize at 40Hz-55Hz for 10min-30min; S5: Weigh the phase D material, dissolve it evenly, add it to the emulsification tank, stir it for 10-30 minutes, let it stand after stirring, and fill it after the prepared liquid is defoamed and stabilized to obtain the sunscreen lotion; Phase A comprises sunscreen, moisturizer, functional ingredients, and purified water, wherein: The sunscreen in phase A is a mixture of methylene bis-benzotriazolyl tetramethylbutylphenol and decyl glucoside; The moisturizer in phase A is a combination of one or more of glycereth-26, butylene glycol, ethylene glycol, propylene glycol, panthenol, and sodium hyaluronate; The active ingredient in phase A is carboxymethyl chitosan; The phase B comprises a sunscreen, an emulsifier and stabilizer, a skin feel regulator, and an emollient, wherein: The sunscreen in phase B is a combination of ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, diethylamino hydroxybenzoyl hexyl benzoate, and ethylhexyl triazone; The emulsifier and stabilizer in phase B is a combination of one or more of arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 glyceryl stearate; The skin feel regulator in phase B is a combination of one or more of polymethylsilsesquioxane, C9-12 alkane, cyclopentasiloxane, and isododecane; The emollient in phase B is cetyl dimethicone; The phase C comprises a skin feel regulator and an emollient, wherein: The skin feel regulator in phase C is a combination of one or more of calcium starch octenylsuccinate and isododecane; The emollient in phase C is a combination of one or more of Natural Bio HB, sorbitol, mannitol, and polyethylene glycol; The D phase contains functional ingredients, fragrance, and purified water, wherein: The active ingredients in phase D are a combination of fish scale collagen and mycosporin-like amino acids; The fragrance in phase D is a perfume.

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