Preparation method of highly active Tripterygium wilfordii extract and its care products and applications

Abstract

The present invention discloses a preparation method of a highly active Tripterygium wilfordii extract and its care products and applications. The highly active Tripterygium wilfordii extract is obtained by extracting the effective ingredients in the Tripterygium wilfordii extract, and is added to the care products to prepare personal care products. The care products contain the following components in weight percentage: 1-15% polyol; 1-5% solubilizer; 0.1-0.5% antioxidant; 1-5% Tripterygium wilfordii extract; 0.5-2% preservative; water to 100%. On the one hand, the present invention verifies that the care product has the effects of being mild, non-irritating and even skin color, and on the other hand, solves the stability problem of the Tripterygium wilfordii extract in the application surface. When the Tripterygium wilfordii extract is used in the formula, it is easily affected by heat and light, and the problem of discoloration can be improved by adding antioxidants and solubilizers, solving the instability problem of the product under light exposure, high temperature environment, etc., and expanding the application scene of the Tripterygium wilfordii extract.

Description

Technical Field

[0001] The present invention relates to a development and preparation method of a nursing product, and in particular to a preparation method of a highly active Tripterygium wilfordii extract and the development and application of the nursing product thereof. Background Art

[0002] With the upgrading of the concept of functional skin care, green and safe have become the core demands of consumers. At the same time, dull skin is the main demand of Asian women. Most of the functional raw materials on the market are chemically synthesized, but there are many factors that cause uneven skin color, such as the cumulative amount of melanin synthesis, increased reactive oxygen free radicals, thinning of the stratum corneum, obstruction of skin blood circulation, exogenous ultraviolet radiation, etc. Therefore, a plant active ingredient that is safe and mild and meets the functional requirements of multiple targets has great market development potential.

[0003] As a traditional Chinese medicine, the plant of the family Tripterygium wilfordii has medicinal effects such as promoting blood circulation and qi circulation, eliminating accumulation and relieving pain, analgesia, and anticoagulation. However, with the research and analysis of the active ingredients of Tripterygium wilfordii extract, the Tripterygium wilfordii extract has also been studied for its efficacy and application in personal care products. Regarding the preparation process of Tripterygium wilfordii, the existing literature data focuses more on mixing and crushing with a variety of traditional Chinese medicines, using dry powder spray, and simple alcohol extraction methods. The literature data related to efficacy are mostly used for the description of analgesia, soothing, and plant antioxidants, and there is no description or record of even skin tone, brightening, and whitening.

[0004] The existing preparation process of Tripterygium wilfordii has problems such as low active ingredient content and insufficient utilization of medicinal materials due to rough process, and there is no specific application research on the process of amplification and the instability of single raw materials. Therefore, a safe and non-irritating plant active ingredient that can be used to even out skin tone has great application prospects in personal care products. Summary of the invention

[0005] In order to solve the deficiencies of the above-mentioned technologies, the present invention provides a preparation method of a highly active Tripterygium wilfordii extract and its care products and applications.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is: a method for preparing a highly active Tripterygium wilfordii extract, wherein the processing and extraction process comprises the following preparation steps:

[0007] S1. The initial Trigonum multiflorum extract is crushed, added into an ethanol aqueous solution for extraction 2 to 3 times, each time adding 8 to 10 times the amount of ethanol aqueous solution, combining the filtrate, and concentrating under reduced pressure to obtain a Trigonum multiflorum extract;

[0008] S2, centrifuging the obtained Tripterygium wilfordii extract to obtain a centrifuge; performing enzymolysis treatments on the centrifuge twice in sequence, at an enzymolysis temperature of 37° C. for 40 to 60 min, and heating the centrifuge to 80 to 90° C. for 20 to 30 min to obtain a Tripterygium wilfordii enzymolysis solution;

[0009] S3. The obtained Trigonella lanceolata enzymatic hydrolysate is subjected to a first column chromatography, the eluate is collected and subjected to a second column chromatography adsorption, the eluate is collected as the target eluate, the target eluate is concentrated under reduced pressure, and filtered to obtain a highly active Trigonella lanceolata extract.

[0010] Preferably, the ethanol mass fraction of the ethanol aqueous solution used for extraction is 30-40%.

[0011] Preferably, in S2, the enzyme selected for enzymatic hydrolysis is one or more of amylase, cellulase, pectinase and protease.

[0012] Preferably, in S3, the first column chromatography filler is a mixture of diatomite and activated carbon particles, and the second column chromatography filler is a non-polar macroporous resin.

[0013] A skin care product containing a highly active Tripterygium wilfordii extract, comprising the following components in percentage by weight:

[0014] Polyol 1-15%;

[0015] Solubilizer 1-5%;

[0016] Antioxidants 0.1-0.5%;

[0017] Tripterygium wilfordii extract 1-5%;

[0018] Preservatives 0.5-2%;

[0019] Water to 100%.

[0020] Preferably, the polyol solvent includes one or more of glycerol, propylene glycol, 1,3-butylene glycol, and 1,2-pentanediol, preferably glycerol and 1,2-pentanediol.

[0021] Preferably, the solvent includes one or more of PEG-40 hydrogenated castor oil, PPG-26-butanediol polyether-26, PEG-60 hydrogenated castor oil, C12-13 parathion-9, polysorbate-20, and poloxamer 184, preferably C12-13 parathion-9.

[0022] Preferably, the ratio of solubilizer to Tripterygium wilfordii extract is ≥ 1:1.

[0023] Preferably, the antioxidant is selected from one or more combinations of sodium sulfite, sodium bisulfite, sodium pyrosulfite, sodium thiosulfate, disodium ethylenediaminetetraacetate, diethylenetriaminepentaphosphoric acid, and sodium glucoheptonate, preferably sodium pyrosulfite and sodium bisulfite.

[0024] Preferably, the preservative is selected from one or more combinations of p-hydroxyacetophenone, caprylhydroxamic acid, chlorphenesin, benzyl alcohol, phenoxyethanol, benzoic acid and its salts, sorbic acid and its salts, and the like.

[0025] The application of care products containing highly active Tripterygium wilfordii extract includes: transparent water-based cosmetics such as aqueous sprays, essences, facial masks, lotions, creams, and various types of leave-on cosmetics.

[0026] The present invention discloses a method for preparing a highly active Trillium extract and a care product and application thereof. The highly active Trillium extract is extracted from the Trillium extract to obtain effective ingredients, and the obtained highly active Trillium extract has mild, whitening, anti-oxidation and anti-inflammatory effects. In addition, in combination with the needs of the cosmetics market, a care product containing the Trillium extract with uniform skin color, high safety and stability is developed. The highly active Trillium extract is added to the care product to obtain a personal care product, providing a new solution for mild antioxidant products. At the same time, the problem of unstable efficacy of the Trillium extract is solved in terms of application. Considering that the Trillium extract is easily affected by heat, light and the pH of the system in the application formula, the instability phenomenon is improved by adding antioxidants and solubilizers, and the instability of the product under light exposure, high temperature environment, etc. is solved, thereby expanding the application scenarios of the Trillium extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a stability test chart of the Tripterygium wilfordii extract of the present invention.

[0028] Figure 2 This is a control chart of the zebrafish blank group in the zebrafish efficacy test.

[0029] Figure 3 This is a control chart of the zebrafish positive group in the zebrafish efficacy test.

[0030] Figure 4 This is a control chart of zebrafish test sample 1 in the zebrafish efficacy test.

[0031] Figure 5 This is a comparison chart of zebrafish test sample 2 in the zebrafish efficacy test.

[0032] Figure 6 This is a comparison chart of the subjects' faces before and after use in human efficacy testing. DETAILED DESCRIPTION

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0034] The present invention aims to extract the active ingredients from the extract of Tripterygium wilfordii and add them to the care products to prepare personal care products. On the one hand, it verifies that the care products have the effect of being mild, non-irritating and even skin tone, and on the other hand, it solves the stability problem of the Tripterygium wilfordii extract in the application surface. When the Tripterygium wilfordii extract is used in the formula, it is easy to be affected by heat and light and change color. The instability phenomenon can be improved by adding antioxidants and solubilizers, solving the instability problem of the product under light exposure, high temperature environment, etc., and expanding the application scenarios of the Tripterygium wilfordii extract.

[0035] First, the processing and extraction process of the Tripterygium wilfordii extract of the present invention comprises the following preparation steps:

[0036] S1. The initial Trigonum multiflorum extract is crushed, added into an ethanol aqueous solution for extraction 2 to 3 times, each time adding 8 to 10 times the amount of ethanol aqueous solution, combining the filtrate, and concentrating under reduced pressure to obtain a Trigonum multiflorum extract;

[0037] S2, centrifuging the obtained Tripterygium wilfordii extract to obtain a centrifuge; performing enzymolysis treatments on the centrifuge twice in sequence, the enzymolysis temperature is 37° C., the time is 40 to 60 min, and after the enzymolysis is completed, the temperature is raised to 80 to 90° C. and maintained for 20 to 30 min to obtain a Tripterygium wilfordii enzymolysis solution; enzymolysis can help decompose starch, protein, pectin and other components in the extract;

[0038] S3. The obtained enzymatic hydrolysate of the Trigonella ovata is subjected to a first column chromatography, the eluate is collected and subjected to a second column chromatography adsorption, the eluate is collected as the target eluate, the target eluate is concentrated under reduced pressure, and filtered to obtain the Trigonella ovata extract; adsorption separation is performed by column chromatography, the purpose of which is to decolorize and purify, so as to increase the content of active ingredients in the Trigonella ovata extract.

[0039] The ethanol mass fraction of the ethanol aqueous solution used for extraction is 30-40%; the enzymes selected for enzymatic hydrolysis are amylase, cellulase, pectinase and protease; the first column chromatography filler is a mixture of diatomite and activated carbon particles, and the second column chromatography filler is non-polar macroporous resin.

[0040] The application forms of the Tripterygium wilfordii extract extracted by the above process in personal care products include: transparent water-based cosmetics such as aqueous sprays or essences or facial masks or lotions and creams, as well as various types of leave-on cosmetics.

[0041] The extract of Tripterygium wilfordii was prepared according to the above-mentioned extraction process of Tripterygium wilfordii extract, and the relevant stability test (90 days) was carried out. Figure 1 From the experimental results, we can see that the color performance data of each experimental group are shown in the following table:

[0042]

[0043] Since the Trillium extract prepared as above contains more plant active ingredients and changes color under light and heat (45°C), in order to solve the application instability of the Trillium extract and product application, different component content ratios and functional ingredient combinations are used to solve the application problems of Trillium in personal care products.

[0044] The aqueous solution stability test scheme of Tripterygium wilfordii extract, the specific components are as follows:

[0045]

[0046] Since the obtained extract of Trillium gracile has certain light and heat discoloration, in order to solve the instability of the application of Trillium gracile extract, different component content ratios and functional ingredients are combined to solve the application problem of Trillium gracile extract in personal care products. The specific components are:

[0047] The polyol solvent includes one or more of glycerol, propylene glycol, 1,3-butylene glycol, and 1,2-pentanediol, preferably glycerol and 1,2-pentanediol;

[0048] The solubilizer includes one or more of PEG-40 hydrogenated castor oil, PPG-26-butanol polyether-26, PEG-60 hydrogenated castor oil, C12-13 polyether-9, polysorbate-20, and poloxamer 184, preferably C12-13 polyether-9, wherein the ratio of the solubilizer to the trifoliate extract is ≥1:1;

[0049] The antioxidant is selected from one or more combinations of sodium sulfite, sodium bisulfite, sodium pyrosulfite, sodium thiosulfate, disodium ethylenediaminetetraacetate, diethylenetriamine pentaerythritol phosphate, sodium glucoheptonic acid, etc., preferably sodium pyrosulfite and sodium bisulfite;

[0050] The preservative is selected from one or more combinations of p-hydroxyacetophenone, caprylhydroxamic acid, chlorphenesin, benzyl alcohol, phenoxyethanol, benzoic acid and its salts, sorbic acid and its salts, etc.;

[0051] Preservatives are commonly used in personal care products and are only used to control the excess of microorganisms in the solution and affect the experimental phenomena.

[0052] The performance of the care products is verified in combination with the embodiments. First, the formula of the care products in each embodiment is shown in the following table:

[0053]

[0054] Experimental steps:

[0055] Weigh the above-mentioned solubilizer and polyol solvent content in turn and add them into a glass beaker, add the Tripterygium wilfordii extract, and heat appropriately (50°C-60°C) until the solution is transparent and dispersed; at 45°C, add antioxidants and appropriate amounts of preservatives, and keep samples for stability observation.

[0056] Similarly, the comparative examples were prepared in the same manner, except that in comparative example 1, the dosage ratio of the solubilizer: the extract of Tripterygium wilfordii was ≤1:1, and in comparative example 3, different antioxidants were selected, and the dosage of the antioxidants in comparative examples 2 and 4 was <0.1%.

[0057] The stability of the examples and comparative examples was observed for 90 days, and the solution stability (90 days) was as shown in the following table:

[0058]

[0059] in conclusion:

[0060] By comparing the stability results of Examples 2 and 4 with Comparative Examples 1 and 3, it is proved that adding a certain amount of solubilizer is beneficial to improving the transparency and stability of the aqueous solution of the Trillium extract. From the experiment, it is recommended to use a ratio of solubilizer to Trillium extract of ≥1:1.

[0061] From the difference in stability between Example 1 and Comparative Examples 2 and 5, it can be seen that adding antioxidants to the solution of the Trillium extract can effectively improve the instability problem of discoloration of the Trillium extract caused by light and heat, making it colorless and transparent, thereby improving the stability of the Trillium extract in the application of skin care products.

[0062] In order to further explore the effect of the pH value of the system on the stability of the aqueous solution of the Sparganium extract, the above Example 2 was selected to observe the stability of the aqueous solution of the Sparganium extract under different pH gradients by adding an acid-base regulator. Among them, citric acid can be used as the acid regulator, and sodium citrate can be used as the alkaline regulator.

[0063] According to the solution of the Tripterygium wilfordii extract of Example 2, an acid-base regulator was added to control the pH in different gradient ranges, namely: 4.0-5.0; 5.0-6.0; 6.0-7.0; 7.0-8.0, wherein the pH of the aqueous solution obtained in Example 2 was 6.46.

[0064] Its stability was observed for 90 days, and the stability was as follows:

[0065]

[0066] The stability of the aqueous solution of Trillium extract under different pH values ​​above proves that in the skin care products added with Trillium extract, the acidic or alkaline conditions of the system have a negative impact on the discoloration of Trillium extract under light exposure and heat conditions, and the discoloration trend deepens, which has a significant impact on its stability. Therefore, it is recommended that the pH range of the system be controlled in a neutral environment of 6-7 for the best stability in the skin care products added with Trillium extract.

[0067] Further, efficacy tests were performed:

[0068] 1. Mildness test

[0069] The chicken embryo chorioallantoic membrane vascular test was used for safety testing to test whether different embodiments have skin irritation. The above embodiments 1-5 were used as test objects. The eggshell of the air chamber part of a nine-day-old chicken embryo was peeled off to expose the white egg membrane, and then the egg membrane was moistened with 0.9% sodium chloride (NaCl) solution, and the inner membrane was removed with tweezers to ensure that the vascular membrane was not damaged. After confirming that the damaged blood vessels were not damaged, a preliminary experiment and a formal experiment were carried out.

[0070] Experimental steps:

[0071] The test groups, each group has at least 6 chicken embryos, each test needs to set up negative control, positive control and blank control;

[0072] Preparation of chick embryo chorioallantoic membrane (CAM): 9-day-old chick embryos were candled, the air chamber eggshell was peeled off, physiological saline was dripped on the surface of the eggshell membrane to fully moisten it, and then the eggshell membrane was removed;

[0073] Apply 5% of the test substance on the surface of the plastic film and turn the film over so that the sample surface is in contact with the CAM;

[0074] After 3 minutes of action, the film was removed and the CAM surface was rinsed with double distilled water. After the rinsing was completed within 30 seconds, the degree of change of each toxic effect was immediately observed and a score (ES) was given.

[0075] Observation results:

[0076] Bleeding: blood flows out of the blood vessels or capillaries of the CAM. No bleeding, mild bleeding, moderate bleeding, and severe bleeding are scored as 0, 1, 2, and 3 points, respectively.

[0077] Coagulation: refers to the denaturation of proteins inside and outside blood vessels, manifested as thrombosis, closure swelling, the appearance of coagulation points inside and outside blood vessels, milky turbidity outside blood vessels, etc. No coagulation, mild coagulation, moderate coagulation, moderate coagulation are awarded 0, 1, 2, and 3 points respectively.

[0078] Vascular ablation: refers to the ablation of blood vessels on the CAM membrane. No ablation, mild ablation, moderate ablation, and severe ablation are scored as 0, 1, 2, and 3 points, respectively.

[0079] Calculate the ES average score according to the following formula and determine the stimulus classification according to the result evaluation table.

[0080]

[0081] The experimental results are shown in the following table:

[0082]

[0083] From the above experimental results, it can be seen that according to the above-mentioned application scheme of the aqueous solution of the Trillium extract, the addition amount is within the range of 1-5%, which is mild, non-irritating and risk-free.

[0084] 2. Zebrafish efficacy test

[0085] This experiment tested the effect of Tripterygium wilfordii extract on zebrafish embryos, and analyzed the changes in the melanin level of fish embryos to determine the melanin inhibition rate, thereby evaluating the inhibitory effect of Tripterygium wilfordii extract on melanin. The test method refers to T / SHRH036-2021 "Cosmetic Melanin Inhibition Test-Zebrafish Embryo Test Method"

[0086] Main reagents:

[0087] 30mg / L phenylthiourea;

[0088] Positive control: 2.5 g / L kojic acid;

[0089] Methyl cellulose solution: weigh 2g to 4g of methyl cellulose and dissolve it in 100mL of water.

[0090] Fish embryo culture medium: 2940 mg of anhydrous calcium chloride, 1233 mg of magnesium sulfate heptahydrate, 630 mg of sodium bicarbonate, and 55 mg of potassium chloride are dissolved in 10 L of water. The pH value is 6.5-8.5.

[0091] Experimental instruments:

[0092] Spawning box / tank, pH meter, dissolved oxygen meter, salinity meter (conductivity meter), brine shrimp incubator

[0093] Test organisms: Spawning tests are conducted using wild-type AB strain zebrafish (Danio rerio) from a reliable source (e.g., China National Zebrafish Resource Center). Broodstock should have good reproductive capacity – the best age is 6 to 12 months.

[0094] Test sample:

[0095] Test sample 1: 0.5% solution of Tripterygium wilfordii extract;

[0096] Test sample 2: 1% Tripterygium wilfordii extract solution;

[0097] Test method:

[0098] 1. Set up groups: It is necessary to set up a blank / solvent control group (fish embryo culture medium / solvent solution), a 100% melanin inhibition model group (phenylthiourea working solution), a positive control group (kojic acid solution) and a test substance group (test substance)

[0099] 2. Test substance treatment: Randomly select 20 fish embryos and place them in a 96-well plate, with each well containing one fish embryo and 0.2 mL of the test substance solution. Place in a 28℃±1℃ incubator and culture until 48h±1h after fertilization.

[0100] 3. Microscopic analysis of samples: For each test, randomly select at least 12 fish embryos, cover the fish embryos with 2% to 4% methylcellulose, and take photos under a microscope. All fish embryos must be photographed with the same photography parameters.

[0101] 4. Data and result calculation: The head and yolk dorsal region of the fish embryo were marked using analysis software, and the “average signal intensity” was selected as the melanin content index.

[0102] Calculate the melanin inhibition rate:

[0103]

[0104] Where:

[0105] S—the average value of “mean signal intensity” of fish embryos in the test substance treatment group;

[0106] B—the average value of “average signal intensity” of fish embryos in the blank control group;

[0107] P—The average value of “mean signal intensity” of fish embryos in the 100% melanin inhibition model group.

[0108] Calculate the mean and standard error of each test group, use statistical software to perform variance analysis on the data, and perform a two-tailed T test on the target melanin signal intensity between the test group and the blank control group (or solvent control group) to obtain the p value. p<0.05 indicates a significant difference.

[0109] Test results:

[0110] The test concentrations of the extract of Tripterygium wilfordii in this experiment were 0.5% and 1%, respectively. The results of the inhibition rate of melanin in zebrafish embryos of the extract of Tripterygium wilfordii were shown in the following table:

[0111]

[0112] Efficacy screening test results such as Figure 2-Figure 5 As shown:

[0113] Figure 2Blank control group: whitening effect is not significant (0% melanin inhibition rate);

[0114] Figure 3 As an example of the positive control group, 2.5 g / L kojic acid was used, which had a significant whitening effect (30% melanin inhibition rate).

[0115] Figure 4 The test sample group 1 used 0.5% Trillium extract, which had a significant whitening effect (17% melanin inhibition rate).

[0116] Figure 5 Test sample group 2, using 1% Trillium extract, had a significant whitening effect (18% melanin inhibition rate).

[0117] From the above test results, it can be seen that the inhibition rates of zebrafish embryo melanin at the addition concentrations of 1% and 0.5% of the Trillium extract were 18% (p=0.000016) and 17% (p=0.000048), respectively, which were significantly different from the blank group, and had a significant effect of inhibiting the production of melanin in zebrafish embryos. Therefore, at a certain concentration, the Trillium extract can reduce the production of melanin, thereby even out skin color and reduce skin pigmentation.

[0118] 3. Human efficacy test

[0119] According to the aqueous solution preparation process in the above Example 1, a 3% aqueous solution of Tripterygium wilfordii extract of quantity*specification 12*30ml was prepared, and volunteers with dark yellow skin and a small amount of spots on the skin were selected for efficacy testing.

[0120] The inclusion criteria for the subjects were as follows:

[0121] The subjects should meet the following criteria:

[0122] ——A healthy male or female aged between 30 and 60 years old;

[0123] ——Those with dark skin, yellowish complexion, or a few spots;

[0124] ——Those who can understand the testing process, voluntarily participate in the trial and sign the informed consent form;

[0125] ——Those who can use the product in the required quantity.

[0126] Subject Exclusion Criteria T / GDCA 009—2022

[0127] The exclusion criteria for subjects are as follows:

[0128] ——Pregnant or breastfeeding women, or those who are planning to get pregnant in the near future;

[0129] ——People with a history of skin diseases such as psoriasis, eczema, atopic dermatitis, etc.;

[0130] - Those who have taken anti-inflammatory drugs such as corticosteroids orally or externally in the past month;

[0131] ——Those who are currently or have participated in other clinical trials in the past month;

[0132] - Participating involuntarily and failing to complete the required content on time;

[0133] ——Other people who are deemed unsuitable to participate in the trial by clinical evaluation.

[0134] Subject restrictions are as follows:

[0135] During the test, the test parts should use the test products provided by the test agency, and no other products with the same efficacy or that may affect the test results should be used. The usage habits of other daily necessities such as soap and shower gel should be kept consistent.

[0136] The environmental conditions are as follows:

[0137] During the test, the test conditions at any test time point should be consistent, such as the tester, location, instrument, etc.; instrument testing should be carried out in an environment with a temperature of (21±1)℃ and a relative humidity of (50±10)%. The subjects should adapt to this environmental condition for at least 30 minutes before evaluation and testing.

[0138] The test site was the face.

[0139] The test instrument is Visia facial image analyzer / skin colorimeter / image acquisition analyzer.

[0140] Test results such as Figure 6 As shown, from left to right are the facial conditions of the subjects before use, on the 7th day, the 14th day, and the 28th day of use. From the facial images of the subjects, we can see that the brightness of their skin has been significantly improved on the 28th day of use, which proves that the plant whitening product with the addition of Tripterygium wilfordii extract has the efficacy of even skin tone and is mild and non-irritating.

[0141] Conclusion: The above experimental tests can prove that Trillium extract is an active ingredient with its own photothermal darkening problem. At the same time, under different test conditions, the application phenomena are also diverse. The test data can clearly indicate that the Trillium extract obtained under the above conditions has multiple functions such as uniform skin color, reduced pigmentation, mild and non-irritating, etc., and has good market prospects.

[0142] At the same time, the present invention solves the application stability of Trillium extract under different pH conditions and provides an optimal neutral range of pH 6-7, suggests optimal application coefficients and different active ingredient combinations for personal care products, solves the application pain points of Trillium extract in various dosage forms of personal care products such as cosmetic water-based solutions and essences, and has good market application prospects.

[0143] The above implementation modes are not limitations of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by technicians in this technical field within the scope of the technical solution of the present invention also belong to the protection scope of the present invention.

Claims

1. A highly active Tripterygium wilfordii extract care product, characterized in that: The care product comprises the following components in percentage by weight: Polyol 1-15%; Solubilizer 1-5%; Antioxidant 0.1-0.3%; Tripterygium wilfordii extract 1-5%; Preservatives 0.5-2%; Water to 100%; The solubilizer is selected from C12-13 parathion-9, and the ratio of the solubilizer to the Tripterygium wilfordii extract is ≥1:1; The antioxidant is selected from sodium bisulfite and sodium pyrosulfite; The highly active Tripterygium wilfordii extract care product system is a neutral environment with a pH range of 6-7; The extraction process of the highly active Tripterygium wilfordii extract comprises the following preparation steps: S1, crushing the raw material of Tripterygium wilfordii, adding it to an ethanol aqueous solution for extraction 2 to 3 times, adding 8 to 10 times the amount of ethanol aqueous solution each time, combining the filtrate, and concentrating under reduced pressure to obtain a Tripterygium wilfordii extract; S2, centrifuging the obtained Tripterygium wilfordii extract to obtain a centrifuge; performing enzymolysis treatments on the centrifuge twice in sequence, at an enzymolysis temperature of 37° C. for 40 to 60 min, and heating the centrifuge to 80 to 90° C. for 20 to 30 min to obtain a Tripterygium wilfordii enzymolysis solution; S3, subjecting the obtained Trigonella lanceolata enzymatic hydrolysate to a first column chromatography, collecting the eluate and subjecting the same to a second column chromatography adsorption, collecting the eluate as a target eluate, concentrating the target eluate under reduced pressure, and filtering to obtain a highly active Trigonella lanceolata extract; In S2, the enzyme selected for enzymatic hydrolysis is one or more of amylase, cellulase, pectinase and protease.

2. The highly active Tripterygium wilfordii extract care product according to claim 1, characterized in that: The polyol includes one or more of glycerol, propylene glycol, 1,3-butylene glycol, and 1,2-pentanediol.

3. The highly active Tripterygium wilfordii extract care product according to claim 2, characterized in that: The polyols are glycerol and 1,2-pentanediol.

4. The highly active Tripterygium wilfordii extract care product according to claim 1, characterized in that: The preservative is selected from one or more combinations of p-hydroxyacetophenone, caprylhydroxamic acid, benzyl alcohol, phenoxyethanol, benzoic acid and its salts, sorbic acid and its salts, etc.

5. The highly active Tripterygium wilfordii extract care product according to claim 1, characterized in that: In S1, the ethanol mass fraction of the ethanol aqueous solution used for extraction is 30-40%.

6. The highly active Tripterygium wilfordii extract care product according to claim 5, characterized in that: In S3, the first column chromatography filler is a mixture of diatomite and activated carbon particles, and the second column chromatography filler is a non-polar macroporous resin.

7. The use of the skin care product containing the highly active Tripterygium wilfordii extract according to claim 6, characterized in that: The application dosage forms of Tripterygium wilfordii extract care products include leave-on cosmetics.

8. The use of the skin care product containing the highly active Tripterygium wilfordii extract according to claim 6, characterized in that: The application dosage forms of the Tripterygium wilfordii extract care products include transparent water-based cosmetics.

9. The use of the skin care product containing the highly active Tripterygium wilfordii extract according to claim 6, characterized in that: The skin care products of Tripterygium wilfordii extract include aqueous spray, essence, facial mask or lotion cream.

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