A decellularization device and a decellularization method

Abstract

The present invention relates to the technical field of general material processing devices, and discloses a decellularization device and a decellularization method, including a shaker body, a constant temperature chamber arranged in the shaker body, an oscillating table installed in the constant temperature chamber, and a sealing cover installed on the shaker body. The present invention uses a secondary drive component to open a guide slot and dock the secondary guide rail with the main guide rail, and then uses the main drive component to unload and move the assembly seat to the secondary guide rail, and finally uses the secondary drive component to move the sample to the secondary control chamber, and closes the guide slot again. This method can realize synchronous oscillation experiments of multiple samples, saving time, and can also automatically transfer and take a single sample during the experiment. In the process of transferring the sample, there is no need to open the sealing cover, which avoids the exchange of external air and air inside the constant temperature chamber, ensures the stability of the temperature inside the constant temperature chamber and the air environment, so that the experimental variables of the sample remain constant, and the experimental results are more accurate.

Description

Technical Field

[0001] The present invention relates to the technical field of general material processing devices, and in particular to a decellularization device and a decellularization method. Background Art

[0002] Before decellularization of a new type of tissue, due to the lack of operating specifications in the past, it is necessary to conduct a preliminary experiment on the tissue first, and determine the accurate oscillation time by detecting the test results, which is used as the final production specification. At present, an oscillating shaker is often used in decellularization technology to achieve decellularization operation. For example, Chinese patent application CN106963985A discloses a decellularization cage and a decellularization device, which improves the cleaning box, improves production efficiency, and simplifies operation. For example, Chinese patent application CN107224612A discloses an automated decellularization system and a decellularization method. In this solution, the control device is used to realize the feeding, oscillation, and discharge after oscillation, which has a high degree of automation, reduces manual intervention, and reduces pollution.

[0003] The above-mentioned oscillating shaker has certain shortcomings in decellularization work: the current oscillating shaker is provided with a single cover and multiple assembly fixtures. If multiple samples are placed inside the shaker for testing at the same time, it is necessary to open the cover at different time periods and then take out the samples for analysis. Opening the cover will cause the external air and the air inside the shaker to exchange, which is easy to destroy the constant temperature environment inside the shaker, and it is also easy to cause external bacteria to enter the shaker, resulting in an increase in experimental variables and interference with the test results. If the samples are placed in the shaker for testing one by one, the time of the entire experiment will be extended several times, and the efficiency is low.

[0004] Currently, no effective solution has been proposed for the problems in the related technologies. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a decellularization device and a decellularization method, which have the advantages of reducing the experimental time in the decellularization experiment while ensuring the stability of the experimental variables, and solves the problem that the existing oscillating shaker causes an increase in experimental variables or a prolongation of the experimental time when conducting tissue decellularization pre-experiments.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a decellularization device, comprising a shaker body, a constant temperature chamber arranged in the shaker body, an oscillating table installed in the constant temperature chamber, and a sealing cover plate installed on the shaker body, wherein a main control chamber is arranged on the back of the shaker body, a secondary control chamber is arranged on the side of the shaker body, the secondary control chamber is connected with the constant temperature chamber through a guide groove, and a main guide rail, a secondary guide rail, an assembly seat, a partition, a main drive assembly and a secondary drive assembly are also arranged in the shaker body;

[0007] The main guide rail is fixed to the edge of the oscillation table, the number of the assembly seats is set to be multiple and all are snap-fitted and mounted on the main guide rail, and the assembly seats are used to install samples;

[0008] The partition is installed on the inner wall of the constant temperature chamber and corresponds to the position of the guide groove, and the partition is used to isolate the constant temperature chamber and the auxiliary control chamber;

[0009] The main drive assembly is installed on one side of the main rail, and the main drive assembly is used to unload and transfer the assembly seat on the main rail;

[0010] The auxiliary guide rail and the auxiliary drive assembly are both installed inside the auxiliary control cavity, the auxiliary guide rail is used to load the assembly seat unloaded from the main guide rail, and the auxiliary drive assembly is used to drive the partition plate and the auxiliary guide rail to move;

[0011] In the decellularization experiment, multiple samples are installed on each assembly seat respectively. When the oscillation table is running, it drives the samples to oscillate. When the sample needs to be taken out, the sub-drive assembly is operated to drive the partition to move and then open the guide slot, and then drive the sub-guide rail to pass through the guide slot and dock with the main rail. The main drive assembly is operated to unload the assembly seat and the sample and transfer them to the sub-guide rail. The sub-drive assembly is operated again, forcing the sub-guide rail and the partition to reset successively, so that the guide slot is closed again after the sample enters the sub-control cavity.

[0012] Preferably, the inner wall of the main rail is provided with multiple groups of limiting holes, the assembly seat includes an assembly slide block slidably connected to the main rail, the top of the assembly slide block is provided with a secondary mounting hole, the assembly slide block is provided with a transverse assembly hole on the side away from the main rail, and the inner wall of the transverse assembly hole is provided with a vertical assembly hole, a transverse pin is arranged inside the transverse assembly hole, a transverse end head is arranged at one end of the transverse pin close to the outside of the transverse assembly hole, a transverse spring is fixed between the transverse end head and the inner wall of the transverse assembly hole, the transverse spring is sleeved on the outside of the transverse pin, and a vertical pin is arranged in the vertical assembly hole, one end of the vertical pin is abutted against the outer wall of the transverse pin, and the other end of the vertical pin is inserted into the limiting hole, a vertical end head is arranged near one end of the vertical pin, a vertical spring is fixed between the vertical end head and the inner wall of the vertical assembly hole, and the vertical spring is sleeved on the outside of the vertical pin.

[0013] Preferably, the side wall of the transverse pin is provided with an annular groove, and an annular slope is provided at one end of the annular groove close to the inside of the transverse assembly hole.

[0014] Preferably, the main control chamber is connected with the constant temperature chamber through a bar-shaped through groove, and the main driving assembly includes a main motor fixed inside the auxiliary control chamber and a guide rod fixed inside the main control chamber, the main motor output shaft is fixed with a main screw, the main screw extends into the main control chamber and both ends are rotatably connected to the inner wall of the main control chamber, a nut seat is threadedly connected to the main screw, the guide rod passes through the nut seat and is clearance-matched with the nut seat, a mounting bracket is fixed to one side of the nut seat, the mounting bracket passes through the bar-shaped through groove and extends into the constant temperature chamber, an electric push rod is fixedly connected to the mounting bracket, and the output end of the electric push rod faces the main rail and is fixed with a push block.

[0015] Preferably, an iron block is fixed to the bottom edge of the assembly slide block, a metal sensor is fixed on the mounting frame, and the metal sensor matches the iron block in height.

[0016] Preferably, the partition is vertically slidably connected to the inner wall of the constant temperature chamber, and a rack is provided on the edge of the partition.

[0017] Preferably, the auxiliary drive assembly includes an auxiliary motor fixed on the inner wall of the auxiliary control chamber, an output shaft is fixed on the output end of the auxiliary motor, the output shaft passes through the inner wall of the auxiliary control chamber and extends into the constant temperature chamber, the output shaft is rotatably connected to the inner wall of the auxiliary control chamber, an output gear is fixed at one end of the output shaft located inside the constant temperature chamber, and the output gear is meshed with a rack.

[0018] The driving mechanism that the cam is in the form of a pin, and the pin is connected with the linking rod at the bottom thereof of the shaft, and the pin is connected with the second end of the driving mechanism, and the pin is connected with the second end of the driving mechanism.

[0019] Preferably, the strip-shaped through groove is arranged in the middle and lower part of the constant temperature chamber side wall, and a ventilation hole is also opened in the middle and upper part of the side wall where the strip-shaped through groove is located. A heat fan is fixed on the inner wall of the main control chamber, and the heat fan corresponds to the position of the ventilation hole.

[0020] The invention also discloses a decellularization method, which uses the above-mentioned decellularization device.

[0021] Compared with the prior art, the present invention provides a decellularization device and a decellularization method, which have the following beneficial effects:

[0022] 1. The decellularization device and decellularization method are provided with a sub-control chamber, a guide slot, a partition, an assembly seat, a main rail, a sub-guide rail, a main drive assembly and a sub-drive assembly. During the tissue decellularization experiment, multiple samples are simultaneously mounted on the assembly seat for oscillation. When a certain sample needs to be taken out, the guide slot is first opened by the sub-drive assembly and the sub-guide rail and the main rail are docked. Then, the assembly seat is unloaded and moved to the sub-guide rail by the main drive assembly. Finally, the sub-drive assembly is used to move the sample into the sub-control chamber and the guide slot is closed again. This method can realize the synchronous oscillation experiment of multiple samples, save time, and can also automatically transfer and take a single sample during the experiment. In the process of transferring the sample, there is no need to open the sealing cover, which avoids the exchange of external air and the air inside the constant temperature chamber, ensures the stability of the temperature inside the constant temperature chamber and the air environment, so that the experimental variables of the sample remain constant, and the experimental results are more accurate.

[0023] 2. This decellularization device and decellularization method, by providing an assembly seat and a main drive component matching the assembly seat, is conducive to unloading and transferring the assembly seat. By providing an iron block on the assembly seat and a metal sensor on the main drive component, it is conducive to accurately selecting the sample to be unloaded according to the number of signals monitored during the movement of the metal sensor. The metal sensor and the iron block can also achieve a positioning effect to ensure that the push block is facing the horizontal assembly hole, thereby improving accuracy, reducing error rate, and making the operation more stable.

[0024] 3. This decellularization equipment and decellularization method are equipped with ventilation holes and a hot fan. When in use, the hot fan runs through the ventilation holes to transport hot air flow into the constant temperature chamber to achieve the purpose of heating. At the same time, the hot air flow can be discharged from the strip grooves below after being discharged from the ventilation holes above, thereby forming a circulating hot air flow, so that the temperature inside the constant temperature chamber is evenly distributed. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of a decellularization device of the present invention;

[0026] Figure 2 It is a structural explosion diagram of the shaking table main body of the present invention;

[0027] Figure 3 A partial cross-sectional view of the oscillating table of the present invention;

[0028] Figure 4is a cross-sectional view of the main guide rail of the present invention;

[0029] Figure 5 For the present invention Figure 4 A magnified view of part A;

[0030] Figure 6 is a cross-sectional view of the rocking table main body of the present invention;

[0031] Figure 7 For the present invention Figure 6 A magnified view of part B;

[0032] Figure 8 It is a structural schematic diagram of the auxiliary driving assembly of the present invention.

[0033] In the figure: 1. Shaking table body; 2. Constant temperature chamber; 3. Oscillating table; 4. Sealing cover plate; 5. Main control chamber; 6. Auxiliary control chamber; 7. Assembly seat; 701. Assembly slide block; 702. Auxiliary mounting hole; 703. Horizontal assembly hole; 704. Vertical assembly hole; 705. Horizontal pin; 706. Horizontal end; 707. Horizontal spring; 708. Vertical pin; 709. Vertical end; 710. Vertical spring; 711. Annular groove; 712. Annular slope; 713. Iron block; 8. Main drive assembly; 801. Main motor; 802. Guide rod; 803. Main screw; 804. Nut seat; 805. Mounting frame; 806. Electric push rod; 807. Push block ;808, metal sensor;9, auxiliary drive assembly;901, auxiliary motor;902, output shaft;903, output gear;904, transmission shaft;905, slide rail;906, fixed slider;907, movable slider;908, auxiliary screw;909, driven slider;910, reset spring;911, extension block;912, bearing seat;913, first bevel gear;914, second bevel gear;915, third bevel gear;916, fourth bevel gear;10, guide groove;11, main rail;111, limit hole;12, auxiliary guide rail;13, partition;131, rack;14, strip groove;15, ventilation hole;16, hot fan. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] As introduced in the background technology, there are deficiencies in the prior art. In order to solve the above technical problems, the present application proposes a decellularization device and a decellularization method.

[0036] Example 1: Please refer to Figure 1-Figure 7 A decellularization device comprises a shaker body 1, a constant temperature chamber 2 arranged in the shaker body 1, an oscillating table 3 installed in the constant temperature chamber 2, and a sealing cover plate 4 installed on the shaker body 1, wherein a main control chamber 5 is arranged on the back of the shaker body 1, and a secondary control chamber 6 is arranged on the side of the shaker body 1, wherein the secondary control chamber 6 is connected with the constant temperature chamber 2 through a guide groove 10, and a main guide rail 11, a secondary guide rail 12, an assembly seat 7, a partition plate 13, a main drive assembly 8, and a secondary drive assembly 9 are also arranged in the shaker body 1;

[0037] The main guide rail 11 is fixed to the edge of the oscillation table 3, the number of the assembly seats 7 is set to be multiple and all are snap-fitted and installed on the main guide rail 11, and the assembly seats 7 are used to install samples;

[0038] The partition 13 is installed on the inner wall of the constant temperature chamber 2 and corresponds to the position of the guide groove 10. The partition 13 is used to isolate the constant temperature chamber 2 and the auxiliary control chamber 6;

[0039] The main drive assembly 8 is installed on one side of the main rail 11, and the main drive assembly 8 is used to unload and transfer the assembly seat 7 on the main rail 11;

[0040] The auxiliary guide rail 12 and the auxiliary driving assembly 9 are both installed inside the auxiliary control cavity 6, the auxiliary guide rail 12 is used to load the assembly seat 7 unloaded from the main guide rail 11, and the auxiliary driving assembly 9 is used to drive the partition 13 and the auxiliary guide rail 12 to move;

[0041] In the decellularization experiment, multiple samples are respectively installed on each assembly seat 7. When the oscillation table 3 is running, the sample is driven to oscillate. When the sample needs to be taken out, the auxiliary driving component 9 is operated to drive the partition 13 to move and then open the guide slot 10, and then drive the auxiliary guide rail 12 to pass through the guide slot 10 and dock with the main guide rail 11. The main driving component 8 is operated to unload the assembly seat 7 and the sample and transfer them to the auxiliary guide rail 12. The auxiliary driving component 9 is operated again, forcing the auxiliary guide rail 12 and the partition 13 to reset successively, so that the guide slot 10 is closed again after the sample enters the auxiliary control chamber 6;

[0042] Among them, the shaking table body 1 is also equipped with a main cover plate and a sub-cover plate that match the main control cavity 5 and the sub-control cavity 6 respectively, wherein the edge of the sub-cover plate is movably connected to the edge of the opening of the sub-control cavity 6 so as to be quickly opened and closed, the main guide rail 11 is fixed to the edge of the oscillation table 3 on the side close to the main control cavity 5, the assembly seat 7 and the oscillation table 3 can be equipped with a clamp, and the sample is fixed by the clamp, the structure and installation method of the clamp are the same as those of the prior art, the main guide rail 11 and the sub-guide rail 12 have the same structure and are in the same direction, and the partition 13 completely covers the guide slot 10 in the initial state;

[0043] When in use, a plurality of samples are fixed on each assembly seat 7 by a clamp, and then the shaking table body 1 is started and the sealing cover plate 4 is closed, and the oscillation table 3 is operated to drive the main rail 11, the assembly seat 7 and the sample to oscillate, and the cell removal operation is performed, and the oscillation is stopped at regular intervals and a sample is taken out, and the sample state is analyzed and recorded. When the sample is taken out, the sealing cover plate 4 is kept closed, and then the auxiliary driving component 9 is started. When the auxiliary driving component 9 is operated, the partition plate 13 is first driven to move, so that the guide slot 10 is opened, and then the auxiliary guide rail 12 is driven to move, and the auxiliary guide rail 12 passes through The guide slot 10 then enters the constant temperature chamber 2, and finally docks with the end of the main rail 11, and then the main drive component 8 is started, and the assembly seat 7 closest to the auxiliary rail 12 is actively driven to be unloaded and transferred along the main rail 11 to the auxiliary rail 12, so that the sample on the assembly seat 7 is transferred to the auxiliary rail 12, and then the auxiliary drive component 9 is started again, and the auxiliary drive component 9 first drives the auxiliary rail 12 to reset, and then transfers the sample to the auxiliary control chamber 6, and then drives the partition 13 to move and reset, and closes the guide slot 10 again, and finally takes out the sample in the auxiliary control chamber 6;

[0044] By setting the auxiliary control chamber 6, the guide groove 10, the partition 13, the assembly seat 7, the main rail 11, the auxiliary rail 12, the main drive component 8 and the auxiliary drive component 9, during the tissue decellularization experiment, multiple samples are simultaneously mounted on the assembly seat 7 for oscillation. When a sample needs to be taken out, the auxiliary drive component 9 is first used to open the guide groove 10 and dock the auxiliary guide rail 12 with the main rail 11, and then the main drive component 8 is used to unload the assembly seat 7 and move it to the auxiliary guide rail 12, and finally the auxiliary drive component 9 is used to move the sample into the auxiliary control chamber 6, and the guide groove 10 is closed again. This method can realize the synchronous oscillation experiment of multiple samples, save time, and can also automatically transfer and take a single sample in the experiment. In the process of transferring the sample, there is no need to open the sealing cover 4, which avoids the exchange of external air and the air inside the constant temperature chamber 2, ensures the stability of the temperature and air environment inside the constant temperature chamber 2, keeps the experimental variables of the sample constant, and makes the experimental results more accurate.

[0045] The inner wall of the main rail 11 is provided with a plurality of groups of limiting holes 111, the assembly seat 7 includes an assembly slider 701 slidably connected to the main rail 11, the top of the assembly slider 701 is provided with a secondary mounting hole 702, the assembly slider 701 is provided with a transverse assembly hole 703 on the side away from the main rail 11, the inner wall of the transverse assembly hole 703 is provided with a vertical assembly hole 704, a transverse pin 705 is provided inside the transverse assembly hole 703, and a transverse end 705 is provided at one end of the transverse pin 705 close to the outside of the transverse assembly hole 703. 6. A transverse spring 707 is fixed between the transverse end 706 and the inner wall of the transverse assembly hole 703. The transverse spring 707 is sleeved on the outer side of the transverse pin 705. A vertical pin 708 is provided in the vertical assembly hole 704. One end of the vertical pin 708 is abutted against the outer wall of the transverse pin 705. The other end of the vertical pin 708 is inserted into the limiting hole 111. A vertical end 709 is provided at one end of the vertical pin 708 close to the transverse pin 705. A vertical spring 710 is fixed between the vertical end 709 and the inner wall of the vertical assembly hole 704. The vertical spring 710 is sleeved on the outside of the vertical pin 708, the side wall of the horizontal pin 705 is provided with an annular groove 711, and the annular groove 711 is provided with an annular slope 712 at one end near the inside of the horizontal assembly hole 703. The main control chamber 5 is connected with the constant temperature chamber 2 through the strip groove 14. The main drive assembly 8 includes a main motor 801 fixed in the auxiliary control chamber 6 and a guide rod 802 fixed in the main control chamber 5. The output shaft 902 of the main motor 801 is fixed with a main screw 803, and the main screw 803 extends The main screw 803 extends into the main control chamber 5 and both ends are rotatably connected to the inner wall of the main control chamber 5. A nut seat 804 is threadedly connected to the main screw 803. The guide rod 802 passes through the nut seat 804 and is in clearance fit with the nut seat 804. A mounting frame 805 is fixed to one side of the nut seat 804. The mounting frame 805 passes through the strip-shaped through slot 14 and extends into the constant temperature chamber 2. An electric push rod 806 is fixedly connected to the mounting frame 805. The output end of the electric push rod 806 faces the main guide rail 11 and is fixed with a push block 807.

[0046] The number of groups of the limiting holes 111 corresponds to the number of the assembly seats 7, and each group of limiting holes 111 is limited to two, and the two limiting holes 111 are symmetrically distributed up and down. The auxiliary mounting hole 702 on the assembly slider 701 is used to install a special fixture for the sample. The oscillation table 3 is provided with a main mounting hole for installing the fixture. The main mounting hole and the auxiliary mounting hole 702 have the same specifications. The vertical pin 708 and the vertical assembly hole 704 are both set to two groups, upper and lower, and are matched with the two limiting holes 111 respectively. The transverse assembly hole 703 includes a thick diameter portion close to the outside and a thin diameter portion close to the inside. The transverse spring 707 is installed on the transverse mounting hole. In the thick diameter portion of the matching hole 703, the vertical assembly hole 704 also includes a thick diameter portion close to the transverse assembly hole 703 and a thin diameter portion away from the transverse assembly hole 703. The vertical spring 710 is installed in the thick diameter portion of the vertical assembly hole 704. The guide rod 802, the main screw 803 and the main rail 11 are distributed in parallel. The strip through groove 14 is in the same direction as the guide rod 802. The height of the push block 807 is consistent with the height of the transverse assembly hole 703. The diameter of the push block 807 is smaller than the maximum inner diameter of the transverse assembly hole 703. When the assembly seat 7 is engaged and installed on the main rail 11, the vertical spring 710 is in a compressed deformation state.

[0047] When in use, the fixture is installed in the mounting hole on the assembly seat 7, and then the sample is installed on the fixture. When the oscillation table 3 is running, the main rail 11 and the assembly seat 7 are driven to shake, and then the fixture and the sample on the fixture are driven to shake. When a sample needs to be taken out, the main motor 801 is started first. When the main motor 801 is running, it drives the main screw 803 to rotate, and then drives the nut seat 804 to move along the main screw 803. When the nut seat 804 moves, it drives the mounting frame 805 and the electric push rod to move along the strip groove 14 until the push block 807 at the end of the electric push rod 806 is opposite to the horizontal assembly hole 703 on the assembly seat 7. Then the electric push rod 806 is started, and the electric push rod 806 is extended, driving the push block 807 to move, so that the push block 807 is inserted into the horizontal assembly hole 703. At this time, the push block 807 contacts and pushes the cross pin 705, When moving, the transverse spring 707 is compressed and moves relative to the vertical pin 708. When the transverse annular groove 711 moves to correspond to the position of the vertical pin 708, the elastic force of the vertical spring 710 pushes the vertical pin 708 to move, so that the vertical pin 708 disengages from the limiting hole 111 and is inserted into the annular groove 711. When the vertical pin 708 disengages from the limiting hole 111, the assembly slider 701 is no longer engaged with the main guide rail 11, and then the main motor 801 is started to drive the nut seat 804, the mounting frame 805, the electric push rod 806 and the push block 807 to move. At this time, since the push block 807 is inserted into the assembly slider 701, the push block 807 drives the assembly slider 701 to slide along the main guide rail 11 when it moves, until the assembly slider 701 slides to the secondary guide rail 12 docking with the main guide rail 11, thereby realizing the unloading and transfer of the assembly seat 7 and the samples on the assembly seat 7.

[0048] By setting an assembly seat 7 that is engaged and connected to the main guide rail 11, the assembly seat 7 can maintain a relatively stable state during the oscillation operation, thereby ensuring the oscillation effect; by setting a main drive component 8 that matches the structure of the assembly seat 7, the vertical pin 708 is first driven by the electric push rod 806 to disengage from the limiting hole 111, which is conducive to contacting the engagement of the assembly seat 7, thereby unloading the assembly seat 7, and then the push block 807 plugged into the assembly seat 7 is driven by the main motor 801 to move, so that the assembly seat 7 and the sample slide along the main guide rail 11, which is conducive to the transfer of the sample; in addition, by setting a transverse spring 707 and an annular slope 712, when the push block 807 disengages from the transverse assembly hole 703, the elastic force of the transverse spring 707 drives the transverse pin 705 to reset, and the annular slope 712 squeezes the end of the vertical pin 708, which can drive the vertical pin 708 to reset again, which is conducive to the repeated use of the assembly seat 7.

[0049] Example 2, see Figure 4-Figure 7 , which is different from the above-mentioned embodiment, an iron block 713 is fixed to the bottom edge of the assembly slider 701, and a metal sensor 808 is fixed to the mounting frame 805, and the metal sensor 808 is highly matched with the iron block 713;

[0050] The metal sensor 808 moves with the mounting bracket 805. When the metal sensor 808 moves to face the iron block 713, the push block 807 at the end of the electric push rod 806 just faces the horizontal assembly hole. When in use, the main drive assembly 8 drives the metal sensor 808, the electric push rod 806 and the push block 807 to move. When the metal sensor 808 moves to face the iron block 713, the metal sensor 808 generates a signal.

[0051] By setting the iron block 713 and the metal sensor 808, it is helpful to accurately select the samples to be unloaded according to the number of signals monitored during the movement of the metal sensor 808, and the metal sensor 808 and the iron block 713 can also achieve a positioning effect to ensure that the push block 807 is facing the horizontal assembly hole 703, thereby improving accuracy, reducing error rate, and making the operation more stable.

[0052] Example 3, see Figure 2 and Figure 8, which is different from the above embodiment, the partition 13 is vertically slidably connected to the inner wall of the constant temperature chamber 2, and a rack 131 is provided on the edge of the partition 13. The auxiliary drive assembly 9 includes an auxiliary motor 901 fixed to the inner wall of the auxiliary control chamber 6, and an output shaft 902 is fixed to the output end of the auxiliary motor 901. The output shaft 902 passes through the inner wall of the auxiliary control chamber 6 and extends into the constant temperature chamber 2. The output shaft 902 is rotatably connected to the inner wall of the auxiliary control chamber 6. The output shaft 902 is located in the constant temperature chamber An output gear 903 is fixed at one end of the interior, and the output gear 903 meshes with the rack 131. The auxiliary drive assembly 9 also includes a transmission shaft 904 and a slide rail 905. A fixed slider 906, a movable slider 907 and an auxiliary screw 908 are provided inside the slide rail 905. The two ends of the auxiliary screw 908 are rotatably connected to the two ends of the slide rail 905 respectively. The fixed slider 906 is sleeved on the outside of the auxiliary screw 908 and is fixedly connected to the slide rail 905. The movable slider 907 The movable slider 907 is threadedly connected to the auxiliary screw 908 and is slidably connected to the slide rail 905. A driven slider 909 is provided between the movable slider 907 and the fixed slider 906. The driven slider 909 is slidably connected to the slide rail 905. A return spring 910 is fixedly connected between the driven slider 909 and the fixed slider 906. The return spring 910 is sleeved on the auxiliary screw 908. An extension block 911 is fixed on the driven slider 909. The auxiliary guide rail 12 is fixed on the extension block 91 1, the transmission shaft 904 is installed on the inner wall of the auxiliary control chamber 6 through the bearing seat 912, the first bevel gear 913 is fixed on the output shaft 902, the second bevel gear 914 is fixed on the top of the transmission shaft 904, the third bevel gear 915 is fixed on the bottom of the transmission shaft 904, and the fourth bevel gear 916 is fixed on one end of the auxiliary screw 908, the second bevel gear 914 is meshed with the first bevel gear 913, and the fourth bevel gear 916 is meshed with the third bevel gear 915;

[0053] Among them, a circular hole with a diameter larger than that of the secondary screw 908 is opened on the fixed slider 906, and the fixed slider 906 is sleeved on the outside of the secondary screw 908 through the circular hole. In the initial state, the position where the output gear 903 meshes with the rack 131 is located on the top side of the rack 131, and a gap is set between the driven slider 909 and the movable slider 907.

[0054] When in use, the auxiliary motor 901 is started, and the auxiliary motor 901 drives the output shaft 902 and the output gear 903 to rotate. When the output gear 903 rotates, it drives the rack 131 and the partition plate 13 to slide upward, and gradually opens the guide slot 10. At the same time, the first bevel gear 913 on the output shaft 902 rotates with the output shaft 902, and also drives the second bevel gear 914 to rotate. When the second bevel gear 914 rotates, it drives the transmission shaft 904 and the third bevel gear 915 to rotate. When the third bevel gear 915 rotates, it drives the fourth bevel gear 916 and the auxiliary screw 908 to rotate. When the auxiliary screw 908 rotates, it drives the movable slider 907 to move. For a period of time After that, the movable slider 907 contacts the driven slider 909 and pushes the driven slider 909. When the driven slider 909 moves, it drives the extension block 911 and the auxiliary guide rail 12 to move, and also compresses the reset spring 910. When the auxiliary guide rail 12 moves, it passes through the opened guide groove 10, and finally the end of the auxiliary guide rail 12 is aligned with the end of the main guide rail 11. After the assembly seat 7 and the sample are transferred to the auxiliary guide rail 12, the auxiliary motor 901 drives the output shaft 902 to rotate in the opposite direction, and the elastic force of the reset spring 910 can drive the auxiliary guide rail 12 and the partition 13 to reset. Finally, when the sample moves to the inside of the auxiliary control chamber 6, the partition 13 also closes the guide groove 10 again.

[0055] By setting up the auxiliary driving component 9, it is beneficial to drive the partition 13 and the auxiliary guide rail 12 to move successively through the auxiliary motor 901, so that the unloaded assembly seat 7 and the sample are first transferred to the auxiliary guide rail 12, and then transferred to the auxiliary control chamber 6. Since there is a sequence in the movement of the partition 13 and the auxiliary guide rail 12, the position conflict of the partition 13 and the auxiliary guide rail 12 or the sample on the auxiliary guide rail 12 is effectively avoided.

[0056] Example 4, see Figure 2 and Figure 6 , which is different from the above embodiment, the strip-shaped through groove 14 is arranged at the middle and lower part of the side wall of the constant temperature chamber 2, and a ventilation hole 15 is also opened at the middle and upper part of the side wall where the strip-shaped through groove 14 is located, and a hot fan 16 is fixed to the inner wall of the main control chamber 5, and the hot fan 16 corresponds to the position of the ventilation hole 15;

[0057] Among them, when the hot fan 16 is running, the exhaust side faces the ventilation hole 15. When in use, the hot fan 16 runs to transport hot air flow into the constant temperature chamber 2 through the ventilation hole 15 to achieve the purpose of heating. At the same time, the hot air flow can be discharged from the strip-shaped through groove 14 below after being discharged from the upper ventilation hole 15, thereby forming a circulating hot air flow, so that the temperature inside the constant temperature chamber 2 is evenly distributed.

[0058] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A decellularization device, comprising a shaker body, a constant temperature chamber arranged in the shaker body, an oscillating table installed in the constant temperature chamber, and a sealing cover plate installed on the shaker body, Features: The back of the shaking table body is provided with a main control chamber, the side of the shaking table body is provided with an auxiliary control chamber, the auxiliary control chamber is connected with the constant temperature chamber through a guide groove, and the shaking table body is also provided with a main rail, an auxiliary rail, an assembly seat, a partition, a main drive assembly and an auxiliary drive assembly; The main guide rail is fixed to the edge of the oscillation table, the number of the assembly seats is set to be multiple and all are snap-fitted and mounted on the main guide rail, and the assembly seats are used to install samples; The partition is installed on the inner wall of the constant temperature chamber and corresponds to the position of the guide groove, and the partition is used to isolate the constant temperature chamber and the auxiliary control chamber; The main drive assembly is installed on one side of the main rail, and the main drive assembly is used to unload and transfer the assembly seat on the main rail; The auxiliary guide rail and the auxiliary drive assembly are both installed inside the auxiliary control cavity, the auxiliary guide rail is used to load the assembly seat unloaded from the main guide rail, and the auxiliary drive assembly is used to drive the partition plate and the auxiliary guide rail to move; The inner wall of the main rail is provided with multiple groups of limiting holes, the assembly seat includes an assembly slide block slidably connected to the main rail, the top of the assembly slide block is provided with a secondary mounting hole, the assembly slide block is provided with a transverse mounting hole on the side away from the main rail, the inner wall of the transverse mounting hole is provided with a vertical mounting hole, a transverse pin is arranged inside the transverse mounting hole, a transverse end head is arranged at one end of the transverse pin close to the outside of the transverse mounting hole, a transverse spring is fixed between the transverse end head and the inner wall of the transverse mounting hole, the transverse spring is sleeved on the outer side of the transverse pin, a vertical pin is arranged in the vertical mounting hole, one end of the vertical pin is abutted against the outer wall of the transverse pin, and the other end of the vertical pin is inserted into the limiting hole, the vertical pin is provided with a vertical end head close to one end of the transverse pin, a vertical spring is fixed between the vertical end head and the inner wall of the vertical mounting hole, and the vertical spring is sleeved on the outer side of the vertical pin; In the decellularization experiment, multiple samples are installed on each assembly seat respectively. When the oscillation table is running, it drives the samples to oscillate. When the sample needs to be taken out, the sub-drive assembly is operated to drive the partition to move and then open the guide slot, and then drive the sub-guide rail to pass through the guide slot and dock with the main rail. The main drive assembly is operated to unload the assembly seat and the sample and transfer them to the sub-guide rail. The sub-drive assembly is operated again, forcing the sub-guide rail and the partition to reset successively, so that the guide slot is closed again after the sample enters the sub-control cavity.

2. A decellularization device according to claim 1, Features: The side wall of the transverse pin is provided with an annular groove, and one end of the annular groove close to the inside of the transverse assembly hole is provided with an annular slope.

3. A decellularization device according to claim 2, Features: The main control chamber is connected with the constant temperature chamber through a strip-shaped through groove, and the main driving assembly includes a main motor fixed inside the auxiliary control chamber and a guide rod fixed inside the main control chamber, a main screw is fixed to the output shaft of the main motor, the main screw extends into the main control chamber and both ends are rotatably connected to the inner wall of the main control chamber, a nut seat is threadedly connected to the main screw, the guide rod passes through the nut seat and is clearance-matched with the nut seat, a mounting frame is fixed to one side of the nut seat, the mounting frame passes through the strip-shaped through groove and extends into the constant temperature chamber, an electric push rod is fixedly connected to the mounting frame, and the output end of the electric push rod faces the main rail and is fixed with a push block.

4. A decellularization device according to claim 3, Features: An iron block is fixed on the bottom edge of the assembly slide block, and a metal sensor is fixed on the mounting frame. The metal sensor is highly matched with the iron block.

5. A decellularization device according to claim 1, Features: The partition is vertically slidably connected to the inner wall of the constant temperature chamber, and a rack is arranged on the edge of the partition.

6. A decellularization device according to claim 5, Features: The auxiliary drive assembly includes an auxiliary motor fixed on the inner wall of the auxiliary control cavity, an output shaft is fixed on the output end of the auxiliary motor, the output shaft passes through the inner wall of the auxiliary control cavity and extends into the constant temperature cavity, the output shaft is rotatably connected to the inner wall of the auxiliary control cavity, an output gear is fixed on one end of the output shaft located inside the constant temperature cavity, and the output gear is meshed with a rack.

7. A decellularization device according to claim 6, Features: The driving mechanism that the cam is in the form of a pin, and the pin is connected to the upper end of the driving mechanism, and the pin is connected to the upper end of the driving mechanism, and the pin is connected to the lower end of the driving mechanism.

8. A decellularization device according to claim 3, Features: The strip-shaped through groove is arranged at the middle and lower part of the side wall of the constant temperature cavity, and a ventilation hole is also opened at the middle and upper part of the side wall where the strip-shaped through groove is located. A heat fan is fixed to the inner wall of the main control cavity, and the heat fan corresponds to the position of the ventilation hole.

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