A medium filtration device

Abstract

The application discloses a culture medium filtering device, which comprises an upper shell and a lower shell, a collecting cylinder provided with a vacuum connecting pipe is connected with a liquid outlet pipe, the lower shell is horizontally and penetratingly connected with a detachable quick-release filter plate provided with a filter membrane, a limiting part for limiting the quick-release filter plate is connected with one side of the lower shell, a gauze pad is arranged in the lower shell and located below the filter membrane, a lead screw motor is connected with a lead screw which is rotatably connected with the inner wall of the lower shell and a sliding rod which is fixedly connected with the inner wall of the lower shell are arranged on both sides of the filter membrane, a cleaning part is arranged above the filter membrane and connected with the lead screw and the sliding rod, a wastewater tank is arranged on one side of the gauze pad, a water inlet part is arranged above the wastewater tank, and the cleaning part reciprocally cleans the impurities on the upper surface of the filter membrane and stays above the wastewater tank, and the cleaning part is washed by the water inlet part. The culture medium filtering device has high filter membrane replacement efficiency, can reduce the influence of air on the culture medium in the lower shell during replacement, can prevent the filter membrane from being blocked, can monitor the bacteria in real time, and improves the filtering quality of the culture medium.

Description

Technical Field

[0001] This invention relates to the field of cell culture equipment technology, and more particularly to a culture medium filtration device. Background Technology

[0002] Cell culture medium is the fundamental substance that supplies nutrients to cultured cells and promotes cell proliferation. It is also the living environment for cell growth and reproduction, generally containing nutrients such as amino acids, vitamins, carbohydrates, and inorganic ions. However, the culture medium may also contain impurities such as microorganisms and bacteria, which can interfere with cell growth and reproduction and affect the purity of the medium. Therefore, filtration is necessary to remove impurities and bacteria. Compared to traditional autoclaving, filtration sterilization minimizes nutrient loss and has become the development direction for culture medium sterilization. The filtration method varies depending on the type of culture medium, culture method, and cell type. Most culture media are filtered using microporous membranes with a pore size of 0.1–0.22 μm to ensure the purity of the culture medium.

[0003] However, existing culture medium filtration devices have some problems in use: on the one hand, the replacement of filter membranes in the filtration device is mostly done manually by laboratory personnel, which is quite troublesome, and the filter mesh is easily clogged by microorganisms, bacteria and other impurities, resulting in a slower filtration speed and reduced filtration efficiency in the later stages of culture medium filtration; on the other hand, during the culture medium filtration process, the lifespan of the filter membrane and whether the culture medium always meets the aseptic filtration requirements cannot be monitored in real time, which makes it impossible to replace the filter membrane in the filtration device in a timely manner and to judge the filtration quality of the culture medium. Summary of the Invention

[0004] To address the technical problems of existing technologies, such as the cumbersome replacement of filter membranes, easy clogging of filter mesh leading to slower filtration speed and reduced filtration efficiency in the later stages of culture medium processing, and the inability to determine the filtration quality of culture medium, this invention provides the following technical solution.

[0005] This invention discloses a culture medium filtration device, comprising an upper shell with an inlet pipe and an inlet chamber, a lower shell connected to the upper shell with an outlet pipe and an outlet chamber, the outlet pipe being connected to a collection cylinder with a vacuum connection pipe, a detachable quick-release filter plate with a filter membrane horizontally inserted into the lower shell, a limiting component for limiting the quick-release filter plate connected to one side of the lower part of the lower shell, a pad mesh located below the filter membrane above the outlet chamber, a lead screw connected to a lead screw motor and fixedly connected to the inner wall of the lower shell on both sides above the filter membrane, a cleaning component connected to the lead screw and the sliding rod above the filter membrane, a wastewater tank on one side of the pad mesh, and a water inlet above the wastewater tank, the cleaning component reciprocatingly cleaning impurities on the upper surface of the filter membrane under the drive of the lead screw motor and settling them above the wastewater tank, and the water inlet rinsing the cleaning component, the lower shell... The quick-release filter plate has a through groove on one side. It includes a frame supporting the filter membrane and a push-pull end with a sealing ring on the outer periphery of the frame that matches the through groove. The lower part of the push-pull end has a limiting groove connected to the limiting component. Below the through groove, there is a receiving groove with a length not less than the length of the through groove. The limiting component includes a limiting plate that is slidably connected to the receiving groove and matches the limiting groove. At least one guide slide rod extending to the lower part of the lower housing is fixedly connected to the lower end of the limiting plate. A pull-down plate is fixedly connected to the lower end of the guide slide rod. A spring located on the outer periphery of the guide slide rod is fixedly connected to the lower end of the lower housing. The length of the limiting plate is not less than the length of the through groove, and the height of the limiting plate is not less than the height of the through groove. When the quick-release filter plate is disengaged from the through groove, the limiting plate can close the through groove, reducing the impact of microorganisms and bacteria in the external air on the inner cavity of the lower housing.

[0006] As a further technical solution, the cleaning component includes a lead screw end connected to the lead screw and a sliding end connected to the slide rod, and a rolling brush is rotatably connected between the lead screw end and the sliding end.

[0007] As a further technical solution, the rolling brush is a flexible wound brush.

[0008] As a further technical solution, a guide groove is provided on the side of the frame away from the wastewater tank, and a guide strip matching the guide groove is provided on the inner wall of the lower shell.

[0009] As a further technical solution, the frame is provided with a positioning protrusion on the side away from the push-pull end, and the inner wall of the lower housing is provided with a positioning groove that matches the positioning protrusion.

[0010] As a further technical solution, the lower end of the outlet pipe is connected to an extension pipe equipped with a solenoid valve, which is connected to the collection cylinder. A bypass pipe is provided on one side of the extension pipe, and a biosensor is connected to the bypass pipe.

[0011] As a further technical solution, the wastewater tank and the water inlet are connected to an external water pump via wastewater pipe and water inlet pipe, respectively.

[0012] As a further technical solution, a camera extending above the filter membrane is sealed and connected to one side of the liquid inlet chamber.

[0013] The beneficial effects of this invention are that the filter membrane can be replaced promptly via a quick-release filter plate and a limiting component. Simply pulling the limiting component allows the quick-release filter plate to be removed for membrane replacement, resulting in high replacement efficiency. Furthermore, during membrane replacement, the limiting plate seals the channel, reducing the impact of air on the culture medium inside the lower housing. During the filtration process, a lead screw motor drives a cleaning component to circulate and clean the filter membrane, preventing clogging. Pure water from the inlet rinses the rolling brush, and the flushed material is then sent to a wastewater tank for centralized treatment. During the filtration process, a camera observes the filter membrane's condition and determines if replacement is necessary. Simultaneously, a biosensor at the bypass pipe monitors bacteria in real time, further improving the filtration quality of the culture medium. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the culture medium filtration device of the present invention;

[0015] Figure 2 This is a schematic diagram of the lower part of the lower shell structure of the culture medium filtration device of the present invention;

[0016] Figure 3 This is an exploded schematic diagram of the upper and lower shells of the culture medium filtration device of the present invention;

[0017] Figure 4 This is a schematic diagram of the quick-release filter plate of the culture medium filtration device of the present invention;

[0018] Figure 5 This is a schematic diagram from another perspective of the quick-release filter plate of the culture medium filtration device of the present invention;

[0019] Figure 6 This is a schematic diagram of the cleaning component of the culture medium filtration device of the present invention;

[0020] Figure 7 This is a cross-sectional schematic diagram of the receiving groove of the culture medium filtration device of the present invention;

[0021] In the diagram: 1-Upper shell; 101-Inlet pipe; 102-Inlet chamber; 2-Lower shell; 201-Outlet pipe; 202-Outlet chamber; 203-Extension pipe; 204-Bypass pipe; 205-Support leg; 206-Guide strip; 207-Positioning groove; 208-Through groove; 209-Wastewater tank; 210-Water inlet section; 211-Mask; 212-Accommodation groove; 3-Collection cylinder; 301-Vacuum connection pipe; 4-Quick-release filter plate; 401-Frame; 402-Filter membrane; 403- Push-pull end; 404-Sealing ring; 405-Limiting groove; 406-Handle; 407-Positioning protrusion; 408-Guide groove; 5-Limiting component; 501-Pull-down plate; 502-Guide slide rod; 503-Spring; 504-Limiting plate; 6-Lead screw motor; 7-Lead screw; 8-Slide rod; 9-Cleaning component; 901-Rolling brush; 902-Lead screw end; 903-Sliding end; 10-Biosensor; 11-Solenoid valve; 12-Camera; 13-Inlet pipe; 14-Wastewater pipe. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. It should be noted that, unless otherwise specified, the embodiments and features described herein can be combined with each other.

[0023] In the description of this invention, it should be understood that the terms "upper" and "lower" are based on the orientation or positional relationship shown in the accompanying drawings and are used only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0024] like Figure 1 and Figure 2 As shown, the present invention discloses a culture medium filtration device, comprising an upper housing 1 having an inlet pipe 101 and an inlet chamber 102, the inlet pipe 101 being connected to the culture medium solution to be filtered. The upper housing 1 is sealed to a lower housing 2 by bolts. The lower housing 2 has multiple support legs 205 at its lower part, and an outlet pipe 201 and an outlet chamber 202 at its lower part. The outlet pipe 201 is connected to a collection cylinder 3, and the upper end of the collection cylinder 3 is provided with a vacuum connection pipe 301, which is connected to an external vacuum pump. The vacuum pump provides negative pressure for the culture medium filtration process.

[0025] In a preferred embodiment, a camera 12 extending above the filter membrane 402 is sealed and connected to one side of the inlet chamber 102. The camera 12 is electrically connected to an external display device, allowing real-time observation of the filter membrane 402's usage. An extension tube 203 connected to the collection cylinder 3 is located at the lower end of the outlet pipe 201. A bypass pipe 204 is provided on one side of the extension tube 203, and a biosensor 10 is connected to the bypass pipe 204. The biosensor 10 can detect microorganisms and bacteria, determining whether bacteria remain in the filtered culture medium for real-time assessment of filtration quality. A solenoid valve 11 is located at the end of the extension tube 203 near the collection cylinder 3. When the biosensor 10 detects bacteria or microorganisms, the solenoid valve 11 immediately closes the extension tube 203, preventing poorly filtered culture medium from entering the collection cylinder 3.

[0026] like Figure 3 , Figure 4 and Figure 5 As shown, in a preferred embodiment, a detachable quick-release filter plate 4 with a filter membrane 402 is horizontally inserted into the lower housing 2. Specifically, a through groove 208 is provided on one side of the lower housing 2, through which the quick-release filter plate 4 can be inserted into the lower housing 2. The quick-release filter plate 4 includes a frame 401 supporting the filter membrane 402. The frame 401 is a frame structure that matches the inner cavity of the lower housing 2, and the filter membrane 402 is snapped or pressed onto it. The filter membrane 402 enters the inner cavity of the lower housing 2 along with the frame 401. The filter membrane 402 is a microporous filter membrane with a pore size of 0.1–0.22 μm, used for filtering microorganisms and bacteria in the culture medium. A pad 211 is provided above the outlet chamber 202 and located below the filter membrane 402. The pad 211 is a large-pore stainless steel filter mesh, used to support the filter membrane 402 while allowing the culture medium solution to flow smoothly down.

[0027] A push-pull end 403 matching the through groove 208 is provided on one side of the frame 401. A handle 406 is provided on the outside of the push-pull end 403. A sealing ring 404 is provided on the outer periphery of the push-pull end 403 facing the through groove 208. A limiting groove 405 connected to the limiting component 5 is provided at the lower part of the push-pull end 403. Pushing the handle 406 pushes the push-pull end 403 into the through groove 208. The frame 401 drives the filter membrane 402 into the inner cavity of the lower housing 2. At this time, the sealing ring 404 seals the through groove 208. The limiting component 5 is inserted into the limiting groove 405 to limit the quick-release filter plate 4, preventing the quick-release filter plate 4 from moving due to negative pressure during vacuuming during filtration.

[0028] A guide groove 408 is provided on the side of the frame 401 away from the wastewater tank 209. A guide strip 206 matching the guide groove 408 is provided on the inner wall of the lower housing 2. The guide strip 206 cooperates with the guide groove 408 to facilitate the smooth entry of the frame 401 into the inner cavity of the lower housing 2. A positioning protrusion 407 is provided on the side of the frame 401 away from the push-pull end 403. A positioning groove 207 matching the positioning protrusion 407 is provided on the inner wall of the lower housing 2 to improve the stability of the frame 401 and the filter membrane 402 during filtration.

[0029] like Figure 3 and Figure 7 As shown, in a preferred embodiment, a limiting component 5 is connected to one side of the lower part of the lower housing 2 to limit the quick-release filter plate 4. Specifically, a receiving groove 212 with a length not less than the length of the through groove 208 is provided below the through groove 208. The limiting component 5 includes a limiting plate 504 that is slidably connected to the receiving groove 212 and matches the limiting groove 405. The length of the limiting plate 504 is not less than the length of the through groove 208, and the height of the limiting plate 504 is not less than the height of the through groove 208. When the quick-release filter plate 4 is disengaged from the through groove 208, the limiting plate 504 can close the through groove 208, reducing the impact of microorganisms and bacteria in the external air on the inner cavity of the lower housing 2.

[0030] At least one guide slide rod 502 extending to the lower part of the lower housing 2 is fixedly connected to the lower end of the limiting plate 504. One end of the guide slide rod 502 is located below the lower housing 2, and the other end slides through the lower housing 2 and extends into the receiving groove 212, where it is fixedly connected to the lower end of the limiting plate 504. A pull-down plate 501 is fixedly connected to the lower end of the guide slide rod 502, and a spring 503 located on the outer periphery of the guide slide rod 502 is fixedly connected to the pull-down plate 501 and the lower end of the lower housing 2. After the quick-release filter plate 4 is inserted into the inner cavity of the lower housing 2, the push-pull end 403 is located in the through groove 208. At this time, under the action of the spring 503, the guide slide rod 502 pushes the limiting plate 504 into the limiting groove 405, so that the quick-release filter plate 4 is fixed to the lower housing 2. When it is necessary to replace the filter membrane 402 on the quick-release filter plate 4, pull the pull plate 501, the limiting plate 504 disengages from the limiting groove 405, and the quick-release filter plate 4 can be pulled out to replace the filter membrane 402. At this time, release the pull plate 501, and under the action of the spring 503, the limiting plate 504 rises to close the through groove 208, reducing the influence of microorganisms and bacteria in the external air on the inner cavity of the lower housing 2.

[0031] like Figure 4 , Figure 5 and Figure 6 As shown, in a preferred embodiment, a lead screw 7 rotatably connected to the inner wall of the lower housing 2 and a slide rod 8 fixedly connected to the inner wall of the lower housing 2 are respectively provided on both sides above the filter membrane 402. The lead screw 7 is connected to a lead screw motor 6 located on the outer side of the lower housing 2, and the lead screw motor 6 drives the rotation of the lead screw 7. A cleaning component 9 connected to the lead screw 7 and the slide rod 8 is provided above the filter membrane 402. The cleaning component 9 includes a lead screw end 902 connected to the lead screw 7 and a sliding end 903 connected to the slide rod 8. A rolling brush 901 is rotatably connected between the lead screw end 902 and the sliding end 903. The rolling brush 901 is a flexible winding brush. During the process of the lead screw motor 6 driving the lead screw 7 to rotate, the rolling brush 901 moves horizontally back and forth while rotating around its own axis, wrapping and scraping away impurities on the upper surface of the filter membrane 402.

[0032] A wastewater tank 209 is provided on one side of the filter screen 211. The rolling brush 901 can move horizontally above the wastewater tank 209. A water inlet 210 is provided above the wastewater tank 209. The water inlet 210 is a cavity arranged along the axial direction of the rolling brush 901, which can spray and rinse the rolling brush 901. The water inlet 210 is connected to an external ordinary water pump or booster water pump, which can rinse the rolling brush 901 with pure water. A wastewater pipe 14 is provided on one side of the wastewater tank 209, which is connected to an external water pump to extract the wastewater in the wastewater tank 209. Driven by the lead screw motor 6, the cleaning component 9 reciprocates to clean the impurities on the upper surface of the filter membrane 402 and keeps them above the wastewater tank 209. The water inlet 210 rinses the cleaning component 9, and after rinsing, it enters the wastewater tank 209. Then, the wastewater in the wastewater tank 209 is extracted by the wastewater pipe 14. Both the inlet pipe 13 and the wastewater pipe 14 are equipped with closed valves to prevent them from affecting the vacuum environment during the filtration process.

[0033] The preferred embodiments and examples of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments and examples. Within the scope of knowledge possessed by those skilled in the art, various changes or equivalent substitutions can be made without departing from the concept of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the scope of protection of the present invention.

Claims

1. A culture medium filtration device, comprising an upper housing (1) having an inlet pipe (101) and an inlet chamber (102), the upper housing (1) being connected to a lower housing (2) having an outlet pipe (201) and an outlet chamber (202), the outlet pipe (201) being connected to a collection cylinder (3) having a vacuum connection pipe (301), characterized in that: The lower housing (2) is horizontally connected to a detachable quick-release filter plate (4) with a filter membrane (402). A limiting component (5) is connected to one side of the lower part of the lower housing (2) to limit the quick-release filter plate (4). A pad (211) is provided above the liquid outlet chamber (202) and located below the filter membrane (402). On both sides above the filter membrane (402), there are screws (7) rotatably connected to the inner wall of the lower housing (2) and connected to a screw motor (6), and slide rods (8) fixedly connected to the inner wall of the lower housing (2). Above the filter membrane (402), there is a connection between the screws (7) and the slide rods (8). The cleaning component (9) has a wastewater tank (209) on one side of the pad mesh (211) and a water inlet (210) above the wastewater tank (209). Driven by the lead screw motor (6), the cleaning component (9) reciprocates to clean the impurities on the upper surface of the filter membrane (402) and keeps them above the wastewater tank (209). The water inlet (210) rinses the cleaning component (9). A through groove (208) is provided on one side of the lower housing (2). The quick-release filter plate (4) includes a frame (401) supporting the filter membrane (402) and an outer periphery located on one side of the frame (401). A push-pull end (403) with a sealing ring (404) that matches the through groove (208) is provided at the lower part of the push-pull end (403), which is connected to the limiting component (5). A receiving groove (212) with a length not less than the length of the through groove (208) is provided below the through groove (208). The limiting component (5) includes a limiting plate (504) that is slidably connected to the receiving groove (212) and matches the limiting groove (405). At least one guide slide rod (502) extending to the lower part of the limiting plate (504) is fixedly connected to the lower end of the limiting plate (504). A pull-down plate (501) is fixedly connected to the lower end of the guide slide rod (502). The pull-down plate (501) is fixedly connected to the lower end of the lower housing (2) by a spring (503) located on the outer periphery of the guide slide rod (502). The length of the limiting plate (504) is not less than the length of the through groove (208), and the height of the limiting plate (504) is not less than the height of the through groove (208). When the quick-release filter plate (4) is disengaged from the through groove (208), the limiting plate (504) can close the through groove (208) to reduce the influence of microorganisms and bacteria in the external air on the inner cavity of the lower housing (2).

2. The culture medium filtration device according to claim 1, characterized in that: The cleaning component (9) includes a lead screw end (902) connected to the lead screw (7) and a sliding end (903) connected to the slide rod (8), and a rolling brush (901) is rotatably connected between the lead screw end (902) and the sliding end (903).

3. The culture medium filtration device according to claim 2, characterized in that: The rolling brush (901) is a flexible winding brush.

4. The culture medium filtration device according to claim 1, characterized in that: The frame (401) has a guide groove (408) on the side away from the wastewater tank (209), and the inner wall of the lower shell (2) has a guide strip (206) that matches the guide groove (408).

5. The culture medium filtration device according to claim 1, characterized in that: The frame (401) has a positioning protrusion (407) on the side away from the push-pull end (403), and the inner wall of the lower housing (2) has a positioning groove (207) that matches the positioning protrusion (407).

6. The culture medium filtration device according to claim 1, characterized in that: The lower end of the outlet pipe (201) is connected to an extension pipe (203) equipped with a solenoid valve (11) connected to the collection cylinder (3). A bypass pipe (204) is provided on one side of the extension pipe (203), and a biosensor (10) is connected to the bypass pipe (204).

7. The culture medium filtration device according to claim 1, characterized in that: The wastewater tank (209) and the water inlet (210) are connected to an external water pump via a wastewater pipe (14) and a water inlet pipe (13), respectively.

8. The culture medium filtration device according to claim 1, characterized in that: A camera (12) extending above the filter membrane (402) is sealed and connected to one side of the liquid inlet chamber (102).

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