A clamping device and method for a chamber on a Transwell plate

Abstract

The present invention discloses a clamping device and method for the upper chamber of a Transwell plate, and relates to the field of Transwell experiments. A first steel rope and a second steel rope are arranged between two clamping plates; a telescopic assembly is arranged on the clamping plate, and the telescopic assembly is used to adjust the height of the first steel rope and the second steel rope; an adjustment assembly is arranged on both clamping plates, and the adjustment assembly is used to adjust the distance between the first steel rope and the second steel rope, so that the first steel rope and the second steel rope clamp the upper chamber of each row in the plate. Researchers can clamp multiple upper chambers in a row of the plate at the same time, avoiding the high time consumption and pollution risks caused by clamping one by one; through the vibration motor, it is conducive to mixing the cells added to the upper chamber, making the cell plating more uniform, and can shake out the bubbles that may be generated during the liquid addition process, so as to ensure the experimental effect; through the detachable upper chamber liquid addition plate, the operation of adding liquid by researchers is convenient, and the experimental efficiency is further improved.

Description

Technical Field

[0001] The invention relates to the field of Transwell, and in particular to a clamping device and method for an upper chamber of a Transwell well plate. Background Art

[0002] Transwell is a cell biology experimental device used to evaluate cell migration or invasiveness. It is mainly composed of a well plate at the bottom and an upper chamber on top that matches its shape. During the experiment, it is necessary to take out the upper chamber and add liquid to the well plate. At present, during the experiment, the operator usually holds it directly by hand or with the help of ordinary medical tweezers, but each has its own limitations: the risk of contamination is high when the upper chamber is held directly by hand, and it is difficult to pick up due to the narrow edge of the upper chamber, and it is easy to fall due to unstable grip; ordinary medical tweezers have a thin head and insufficient material strength, so the clamping process may be unstable and cause the upper chamber to overturn, resulting in a waste of resources and time.

[0003] At the same time, most transwell experiments require the addition of serum-free culture medium and cell suspension to the upper chamber at the same time, so proper mixing is required. However, manual shaking is usually used for mixing, and there is no special method for shaking and mixing liquids. In addition, bubbles may be generated during the addition process due to various reasons, which will have a negative impact on the experimental results.

[0004] Secondly, when adding serum-free culture medium and cells, the operator's hands are likely to appear above the upper chamber, bringing potential contamination risks and affecting the experimental results. Summary of the invention

[0005] An object of the present invention is to provide a clamping device and method for an upper chamber of a Transwell plate, by which the upper chamber can be clamped and moved quickly and efficiently.

[0006] This purpose is achieved by adopting the following technical solutions:

[0007] A clamping device and method for a Transwell orifice plate upper chamber, comprising a return plate located above the orifice plate, the return plate being a return-shaped structure with a hollow structure in the middle, and the two sides of the return plate being the two sides of the upper hollow structure of the return plate. Both sides of the return plate are provided with first slide grooves, and the two first slide grooves are provided with a clamping structure; the clamping structure comprises two clamping plates respectively located on the two first slide grooves, and a first steel rope and a second steel rope are provided between the two clamping plates.

[0008] When in use, the circular plate is located above the orifice plate, and the lower chambers on the orifice plate are all located in the hollow structure of the circular plate. Adjustment components are provided on the two clamping plates, and the adjustment components are used to adjust the distance between the first steel rope and the second steel rope so that the first steel rope and the second steel rope clamp the upper chamber of each row in the orifice plate.

[0009] At the same time, the clamping plate can slide on the corresponding first sliding groove, so that the first steel rope and the second steel rope are located above each row of upper chambers in the orifice plate.

[0010] In addition, a telescopic component is arranged on the clamping plate, and the telescopic component is used to adjust the height of the first steel rope and the second steel rope.

[0011] Transwell is a cell biology experimental device for evaluating cell migration or invasiveness. It is mainly composed of a lower chamber on the lower well plate and an upper chamber on it that matches its morphology. During the experiment, it is necessary to take out the upper chamber and add liquid to the well plate and other related operations. The existing clamping device usually uses tweezers to clamp the upper chamber and move it to the lower chamber where it needs to be placed. During the clamping process, the clamping may be unstable and the upper chamber may overturn, resulting in a waste of resources and time. At the same time, during the experiment, the operator usually designs a whole row of upper chambers as experimental subgroups, and needs to remove the whole row of upper chambers to add liquid to the lower chamber. At present, the operator can only clamp and place them one by one when clamping, which is inefficient. Therefore, the clamping device of the present device can clamp a row of upper chambers at the same time, and with the help of the adjustment component and the telescopic component, the upper chamber of a row is raised and moved as a whole and placed in the adjacent lower chamber. And during the operation, the operator's hand will not touch the upper chamber, nor will it appear above the upper chamber, which can minimize the risk of contamination.

[0012] When in use, the clamping plate slides on the first slide groove to move the first steel rope and the second steel rope to the top of the upper chamber to be taken out, and the distance between the first steel rope and the second steel rope is the original length. The height of the first steel rope and the second steel rope is adjusted by the telescopic component, so that the first steel rope and the second steel rope are in contact with the top of the orifice plate, and the first steel rope and the second steel rope are located above the orifice plate, and the upper chamber to be taken out is located between the first steel rope and the second steel rope, and the distance between the first steel rope and the second steel rope is adjusted by the adjustment component, so that the first steel rope and the second steel rope are inserted into the gap between the upper chamber to be taken out and the lower chamber to complete the clamping of the upper chamber to be taken out, and the height of the first steel rope and the second steel rope is adjusted again, so that the first steel rope and the second steel rope drive the upper chamber to be clamped to move upward, and the clamping plate is moved again, so that the clamping plate slides on the first slide groove, and the upper chamber is moved to the top of the lower chamber to be placed, and the height is adjusted, and the first steel rope and the second steel rope place the clamped upper chamber in the corresponding lower chamber.

[0013] The adjusting component can be of various structures, as long as it can adjust the distance between the first steel rope and the second steel rope. In the present device, the inventor preferably selects a structure of the adjusting component.

[0014] Specifically, the adjustment component includes a third slide groove and two adjusting wheels arranged on the two clamping plates, two acting wheels are arranged on the third slide groove, and an adjusting member is arranged on the acting wheel, and the adjusting member can adjust the position of the acting wheel on the corresponding third slide groove; the first steel rope corresponds to an acting wheel and an adjusting wheel on the two clamping plates, and the two ends of the first steel rope are respectively connected to the corresponding two adjusting wheels, and the corresponding two acting wheels act on the first steel rope; the second steel rope corresponds to another acting wheel and another adjusting wheel on the two clamping plates, and the two ends of the second steel rope are respectively connected to the corresponding two adjusting wheels, and the corresponding two acting wheels act on the second steel rope; the four acting wheels move on the third slide groove to adjust the distance between the first steel rope and the second steel rope above the upper chamber of the orifice plate.

[0015] The two ends of the first steel rope are respectively connected to the two adjusting wheels, the first steel rope contacts the two action wheels, and the position of the first steel rope on the third slide groove is adjusted by sliding on the third slide groove through the two action wheels. The two ends of the second steel rope are respectively connected to the two adjusting wheels, the second steel rope contacts the two action wheels, and the position of the second steel rope on the third slide groove is adjusted by sliding on the third slide groove through the two action wheels.

[0016] The adjusting wheel is provided with a torsion spring, which acts on the adjusting wheel to keep the first steel rope and the second steel rope in a straight state.

[0017] For this device, the distance between the first steel rope and the second steel rope needs to be adjusted because the edge of the upper chamber is narrow. After the upper chamber is placed in the lower chamber, there is a gap between the upper end of the upper chamber and the upper end of the lower chamber. Therefore, the function of the adjustment component is to fine-tune the distance between the first steel rope and the second steel rope. When the first steel rope and the second steel rope are located on both sides of the upper chamber, the first steel rope and the second steel rope are located between two adjacent rows of lower chambers. The distance between the first steel rope and the second steel rope is then adjusted by the adjustment component so that the first steel rope and the second steel rope are respectively inserted into the gap between the upper chamber and the lower chamber that need to be clamped, thereby achieving tight clamping.

[0018] Therefore, the adjustment component acts on the first steel rope and the second steel rope, and the adjustment distance is short, and there is no need for long-distance adjustment. Secondly, the first steel rope and the second steel rope remain in a straight state, which is more conducive to the first steel rope and the second steel rope acting on the upper chamber and better clamping it.

[0019] When the adjustment component of the device is in operation, when the two action wheels on the first steel rope move toward the direction close to the second steel rope, the distance between the first steel rope and the second steel rope is shortened. At this time, the length of the first steel rope between the two action wheels on the first steel rope remains unchanged, and the length of the first steel rope between the action wheel and the adjustment wheel on the first steel rope becomes longer. Therefore, in this process, the adjustment wheel rotates to release the length of the first steel rope.

[0020] When the two action wheels on the first steel rope move away from the second steel rope, the distance between the first steel rope and the second steel rope increases. At this time, the length of the first steel rope between the two action wheels on the first steel rope remains unchanged, and the length of the first steel rope between the action wheel and the adjusting wheel on the first steel rope becomes shorter. During this process, the adjusting wheel rotates to recover the length of the first steel rope.

[0021] The adjustment assembly has the same action mechanism on the first steel rope and the second steel rope.

[0022] Furthermore, two auxiliary wheels are arranged on the two third slide grooves, and the two auxiliary wheels are connected to the clamping plate through a spring rod, and the spring rod makes the two auxiliary wheels contact the first steel rope and the second steel rope respectively. The first steel rope or the second steel rope is clamped between the auxiliary wheel and the action wheel, so that the first steel rope and the second steel rope are more stable when clamping the upper chamber.

[0023] On the other hand, a partition plate is provided at the lower end of the clamping plate, a first telescopic rod is provided between the partition plate and the clamping plate, a first connecting rod is provided at the lower end of the partition plate, the first connecting rod is located in the first sliding groove, and the first connecting rod can slide in the first sliding groove; the telescopic component is a second telescopic rod provided between the partition plate and the clamping plate or a pulling ring provided on the side of the clamping plate.

[0024] For this device, the first telescopic rod can be extended and retracted by the extension of the second telescopic rod to raise the clamping plate. The extension lengths of the second telescopic rods on the two clamping plates are the same, so the heights of the two clamping plates can be ensured to be the same. The two clamping plates can also be raised by the operator lifting the lifting ring to drive the extension and retraction of the first telescopic rod.

[0025] Secondly, the auxiliary wheels are all provided with a third connecting rod, and the third connecting rod is provided with a connecting sleeve, which is used to pass through the first steel rope or the second steel rope. Most transwell experiments require adding serum-free culture medium and cells to the upper chamber at the same time, and they need to be properly mixed after adding, but currently manual mixing is usually used. During the manual mixing process of the operator, the liquid is easy to splash or hang on the wall, affecting the experimental results.

[0026] The connecting sleeve on the auxiliary wheel of the device passes through the first steel rope or the second steel rope, which can further guide it. At the same time, a micro vibration motor is arranged on the third connecting rod. The first steel rope and the second steel rope transmit vibrations through the steel ropes, which can have a good effect on the upper chamber in contact with the first steel rope and the second steel rope, and can effectively mix the liquid and cells in the upper chamber, avoiding the risk of liquid splashing and hanging on the wall caused by manual shaking. At the same time, bubbles can also be shaken out to avoid affecting the experimental results.

[0027] At the same time, the auxiliary wheel is connected to the clamping plate through a spring rod, so when the micro vibration motor is working, the spring rod has a buffering effect on the auxiliary plate to achieve a better vibration effect.

[0028] On the other hand, in the transwell experiment, in addition to clamping the upper chamber, it is also necessary to add materials to the upper chamber. When adding materials, if the operator's hands are located above the upper chamber, it is easy to cause contamination to the liquid and cells in the upper chamber. Therefore, the present invention also provides a second slide groove on both sides of the return plate, and the two second slide grooves are detachably connected with a feeding structure, the feeding structure includes a feeding plate, and a plurality of feeding holes are provided on the feeding plate. The plurality of feeding holes correspond one by one to the lower chambers of each row in the orifice plate, and the feeding plate can slide in the two second slide grooves so that the feeding holes correspond to the lower chambers in each row respectively.

[0029] An auxiliary part is arranged in the feeding hole, the inner diameter of the auxiliary part gradually decreases from top to bottom, and a fixing hole is arranged at the lower end of the auxiliary part.

[0030] When materials need to be added, the operator inserts the lower end of the feeding tube into the fixing hole. The diameter of the fixing hole is small, so the feeding tube can be initially fixed. The operator can quickly add materials through the fixing hole, and the auxiliary parts will also shield the operator's hands to avoid contamination of the upper chamber.

[0031] Further, a clamping method for the upper chamber of a Transwell plate comprises the following steps:

[0032] Place the return plate above the orifice plate, and adjust the position of the clamping plate in the first slide slot so that the first steel rope and the second steel rope are located above the upper chamber to be clamped;

[0033] The height of the first steel rope and the second steel rope is adjusted by the telescopic assembly so that the first steel rope and the second steel rope are located above the lower chamber corresponding to the upper chamber to be clamped;

[0034] The adjustment assembly shortens the gap between the first steel rope and the second steel rope so that the first steel rope and the second steel rope are located at the gap between the corresponding upper chamber and the lower chamber;

[0035] The height of the first steel rope and the second steel rope are adjusted by the telescopic assembly so that the first steel rope and the second steel rope take out the corresponding upper chamber;

[0036] Adjust the position of the clamping plate in the first slide groove so that the first steel rope and the second steel rope are located above the lower chamber where the upper chamber is placed;

[0037] The heights of the first steel rope and the second steel rope are adjusted by the telescopic assembly so that the taken-out upper chamber is located in the corresponding lower chamber.

[0038] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0039] The present invention discloses a clamping device and method for upper chambers of a Transwell orifice plate. The device can clamp a row of upper chambers at the same time, and each upper chamber does not need to be clamped separately, thereby improving the clamping efficiency. During the clamping process, the first steel rope and the second steel rope interact with each other, and the two clamping plates are located on both sides of the orifice plate. The operator's hands do not appear above the orifice plate, which can effectively avoid contamination.

[0040] Secondly, the device vibrates the upper chamber simultaneously through the first steel rope and the second steel rope, thereby improving the mixing efficiency and being more conducive to operation and use.

[0041] In addition, the feeding structure of the device controls the feeding height and position of the feeding pipe through auxiliary parts to avoid spilling and also prevent other materials from entering during feeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] The drawings described herein are used to provide a further understanding of the embodiments of the present invention, constitute a part of this application, and do not constitute a limitation of the embodiments of the present invention. In the drawings:

[0043] Figure 1 It is a schematic diagram of the return plate structure;

[0044] Figure 2 The schematic diagram of the structure of the adjustment component in the original state;

[0045] Figure 3 It is a structural schematic diagram of the adjustment assembly when the distance between the first steel rope and the second steel rope is shortened;

[0046] Figure 4 It is a structural schematic diagram of the action wheel;

[0047] Figure 5 It is a schematic diagram of the structure of the clamping plate and the partition plate;

[0048] Figure 6 It is a structural schematic diagram of the device when the first steel rope and the second steel rope correspond to the uppermost row of upper chambers;

[0049] Figure 7 It is a structural schematic diagram of the device when the first steel rope and the second steel rope correspond to the lowermost row of upper chambers;

[0050] Figure 8 This is a schematic diagram of the structure of the micro vibration motor on the auxiliary wheel;

[0051] Fig. 9 It is a structural schematic diagram of the feeding structure;

[0052] Fig.10 It is a schematic diagram of the structure of the auxiliary parts;

[0053] Fig.11 It is a structural schematic diagram of the feeding plate moving to the top of the corresponding upper chamber.

[0054] Marks and corresponding parts names in the attached drawings:

[0055] 1-return plate, 2-first slide groove, 3-clamping plate, 31-first telescopic rod, 32-partition plate, 33-first connecting rod, 4-third slide groove, 5-action wheel, 51-first connecting groove, 52-first clamping plate, 53-adjusting member, 6-adjusting wheel, 7-first steel rope, 8-second steel rope, 9-auxiliary wheel, 91-second connecting groove, 92-second clamping plate, 93-spring rod, 94-micro vibration motor, 95-third connecting rod, 96-connecting sleeve, 10-lifting ring, 11-second slide groove, 12-feeding plate, 121-second connecting rod, 13-feeding hole, 14-auxiliary member, 15-first support column. DETAILED DESCRIPTION

[0056] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments and drawings. The exemplary embodiments of the present invention and their description are only used to explain the present invention and are not intended to limit the present invention.

[0057] In the description of the present invention, it should be understood that the terms "front", "rear", "left", "right", "up", "down", "vertical", "horizontal", "high", "low", "inside", "outside", etc., indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the scope of protection of the present invention.

[0058] Example 1

[0059] like Figure 1 As shown, it includes a circular plate 1 located above the well plate, and four first support columns 15 are arranged at the lower end of the circular plate 1. The four first support columns 15 are used to support the circular plate 1. The Transwell well plate is located in the four first support columns 15, and the side surfaces of the Transwell well plate are in contact with two of the first support columns 15.

[0060] The hollow structure in the circular plate 1 is located above the Transwell plate. For the Transwell plate, it is usually a Transwell plate with 24 lower chambers, that is, it has four rows, and each row has six lower chambers. This embodiment is described by taking the Transwell plate with 24 lower chambers as an example, but the size of the specific Transwell plate and the number of lower chambers are not specifically limited.

[0061] In this embodiment, when the circular plate is located above the Transwell plate, the 24 lower chambers are all located in the hollow structure.

[0062] First slide grooves 2 are provided on both sides of the upper end surface of the return plate 1, that is, first slide grooves 2 are provided on both sides of the hollow structure of the return plate 1, and the direction of the first slide grooves is parallel to the direction of each column of lower chambers.

[0063] A clamping structure is provided on the two first chutes 2 ; the clamping structure comprises two clamping plates 3 respectively located on the two first chutes 2 , and a first steel rope 7 and a second steel rope 8 are provided between the two clamping plates 3 .

[0064] In the original state, the distance between the first steel rope 7 and the second steel rope 8 is outside the extension of the upper chamber of the orifice plate, and the first steel rope 7 and the second steel rope are located at the upper and lower ends of a row of upper chambers.

[0065] When clamping the upper chamber, the adjustment assembly shortens the distance between the first steel rope 7 and the second steel rope 8, so that the first steel rope and the second steel rope are embedded in the gap formed between the upper and lower ends of the upper chamber and the lower chamber.

[0066] At the same time, the clamping plate 3 can slide on the corresponding first slide groove 2, so that the first steel rope 7 and the second steel rope 8 are located above each row of upper chambers in the orifice plate, corresponding to each row of upper chambers respectively, and each row of upper chambers can be flexibly moved to the lower chambers of another row.

[0067] Secondly, a telescopic assembly is provided on the clamping plate 3, and the telescopic assembly is used to adjust the height of the first steel rope 7 and the second steel rope 8, so that the first steel rope and the second steel rope can take out and put down the upper chamber of each row after clamping.

[0068] Among them, the adjustment component is used for the distance between the first steel rope 7 and the second steel rope 8. The adjustment component can be of various structures. For example, the distance between the first steel rope 7 and the second steel rope 8 can be adjusted by moving the pulley; the distance between the first steel rope 7 and the second steel rope 8 can also be adjusted by a telescopic structure, as long as the distance between the first steel rope 7 and the second steel rope 8 can be adjusted.

[0069] Example 2

[0070] On the basis of Example 1, a structure of an adjustment component is preferred, the adjustment component comprises a third slide groove 4 and two adjustment wheels 6 arranged on two clamping plates 3, the two adjustment wheels 6 are located at the upper and lower ends of the clamping plates 3; two action wheels 5 are arranged on the third slide groove 4, and the action wheels 5 are provided with adjustment members 53, and the adjustment members 53 can adjust the position of the action wheels 5 on the corresponding third slide groove 4; the action wheels 5 on the two third slide grooves 4 are located between the adjustment wheels 6 on the two clamping plates 3;

[0071] The two ends of the first steel rope 7 are respectively connected to the two adjusting wheels 6 located at the upper end of the clamping plate 3, and the two action wheels 5 at the upper ends of the two third slide slots 4 act on the first steel rope 7, so that the first steel rope 7 includes two vertical sections and one horizontal section;

[0072] The two ends of the second steel rope 8 are respectively connected to the two adjusting wheels 6 located at the lower end of the clamping plate 3, and the two acting wheels 5 at the lower ends of the two third slide slots 4 act on the second steel rope 8, so that the second steel rope 8 includes two vertical sections and one horizontal section;

[0073] The four action wheels 5 move on the third slide groove 4 to adjust the distance between the first steel rope 7 and the second steel rope 8 above the upper chamber of the orifice plate.

[0074] In the original state, the adjustment components are as follows Figure 2 As shown, the distance between the first steel rope 7 and the second steel rope 8 is the extension of the upper chamber of the orifice plate. At this time, the straight line where the two action wheels 5 on the first steel rope 7 are located is a horizontal straight line, the straight line where the two adjustment wheels 6 on the first steel rope 7 are located is a horizontal straight line, and the straight line where the first steel rope between the two action wheels is also a horizontal straight line. The straight line where the two action wheels 5 on the second steel rope 8 are located is a horizontal straight line, the straight line where the two adjustment wheels 6 on the second steel rope 8 are located is a horizontal straight line, and the straight line where the second steel rope between the two action wheels is also a horizontal straight line.

[0075] The four action wheels 5 move on the third slide 4 to adjust the distance between the first steel rope 7 and the second steel rope 8 above the upper chamber of the orifice plate. The adjusting member is a third telescopic rod, and the two action wheels 5 acting on the first steel rope 7 are connected to one end of the clamping plate 3 through the third telescopic rod, and the two action wheels 5 acting on the second steel rope 8 are connected to the other end of the clamping plate 3 through the third telescopic rod. The third telescopic rod is extended and retracted to make the action wheels on the first steel rope 7 and the second steel rope 8 approach or move away from each other.

[0076] When the upper chamber needs to be clamped, the adjustment assembly shortens the distance between the first steel rope 7 and the second steel rope 8, such as Figure 3 As shown, that is, based on the original state, the third telescopic rod is extended, the action wheels on the first steel rope 7 and the second steel rope 8 move toward each other, and the distance between the first steel rope 7 and the second steel rope 8 is shortened, so that the first steel rope and the second steel rope are respectively clamped in the gap between the upper chamber and the lower chamber that need to be clamped.

[0077] The adjusting wheel 6 is provided with a torsion spring, which acts on the adjusting wheel 6 to keep the first steel rope 7 and the second steel rope 8 in a straight state. When the distance between the first steel rope 7 and the second steel rope 8 is shortened, the length of the first steel rope and the second steel rope wound on the adjusting wheel 6 is reduced. In the original state, the length of the first steel rope and the second steel rope wound on the adjusting wheel 6 is greater than the length of the first steel rope and the second steel rope wound on the adjusting wheel 6 when the distance between the first steel rope 7 and the second steel rope 8 is shortened.

[0078] Among them, the action wheel 5 is as follows Figure 4 As shown, a first connecting groove 51 is provided on the side of the action wheel 5. When the action wheel 5 acts on the first steel rope 7 or the second steel rope 8, the first steel rope 7 or the second steel rope 8 is located in the first connecting groove 51. A first clamping plate 52 is provided at the lower end of the action wheel 5. The first clamping plate 52 is located below the clamping plate 3.

[0079] like Figure 5 As shown, a partition plate 32 is provided at the lower end of the clamping plate 3, a first telescopic rod 31 is provided between the partition plate 32 and the clamping plate 3, a first connecting rod 33 is provided at the lower end of the partition plate 32, the first connecting rod 33 is located in the first chute 2, and the first connecting rod 33 can slide in the first chute 2; the telescopic assembly is a second telescopic rod provided between the partition plate 32 and the clamping plate 3 or a lifting ring 10 provided on the side of the clamping plate 3. Two auxiliary wheels 9 are provided on each of the two third chute 4, and the two auxiliary wheels 9 are connected to the clamping plate 3 through a spring rod 93, and the two auxiliary wheels 9 on the third chute 4 are in contact with the first steel rope 7 and the second steel rope 8 respectively. A second clamping plate 92 is provided at the lower end of the auxiliary wheel 9, and the second clamping plate 92 is located below the clamping plate 3. A second connecting groove 91 is provided on the side of the auxiliary wheel 9, and when the auxiliary wheel 9 acts on the first steel rope 7 or the second steel rope 8, the first steel rope 7 or the second steel rope 8 is located in the second connecting groove 91.

[0080] The first clamping plate 52 is connected to the first telescopic rod 31 via an adjusting member 53 , and the second clamping plate 92 is connected to the first telescopic rod 31 via a spring rod 93 .

[0081] In some embodiments, the telescopic component is a second telescopic rod arranged between the partition plate 32 and the clamping plate 3. When the operator needs to remove the upper chamber clamp, the first steel rope and the second steel rope clamp the upper chamber, and the second telescopic rod extends, driving the first telescopic rod to extend, and supporting the clamping plate to the upper position, wherein the telescopic lengths of the second telescopic rods on the two clamping plates are fixed and the same, thereby ensuring that the two clamping plates are lifted to the same height.

[0082] In some embodiments, the telescopic component is a lifting ring 10 provided on the side of the clamping plate 3. When the operator needs to remove the upper chamber clamp, after the first steel rope and the second steel rope clamp the upper chamber, the operator pulls the lifting rings 10 on the sides of the two clamping plates 3 with both hands to quickly raise it. This structure is convenient and fast to operate.

[0083] As for the first connecting rod 33 being located in the first sliding groove 2 and being able to slide in the first sliding groove 2, there can be a variety of structures, such as sliding through a pulley connection, and sliding through gear rotation, as long as the first connecting rod 33 can slide in the first sliding groove 2.

[0084] When in use, when the first steel rope and the second steel rope correspond to the uppermost row of upper chambers, Figure 6 As shown, when the first connecting rod 33 slides in the first sliding groove 2, the first steel rope and the second steel rope correspond to the lowermost row of upper chambers, as shown in FIG. Figure 7 shown.

[0085] Example 3

[0086] On the basis of the above embodiment, the two auxiliary wheels 9 acting on the first steel rope 7 are connected to one end of the clamping plate 3 via a spring rod 93, and the two auxiliary wheels 9 acting on the second steel rope 8 are connected to the other end of the clamping plate 3 via a spring rod 93. In the original state, the two auxiliary wheels 9 on the third slide groove 4 are not in contact. Under the action of the two spring rods, the two auxiliary wheels 9 on the third slide groove 4 are in contact with the first steel rope 7 and the second steel rope 8 respectively. When the third telescopic rod is extended and the distance between the first steel rope 7 and the second steel rope 8 is shortened, the two auxiliary wheels 9 on the third slide groove 4 are in contact and the spring rod is extended.

[0087] In this embodiment, if Figure 8 As shown, the auxiliary wheels 9 are each provided with a third connecting rod 95 , the third connecting rod 95 is provided with a connecting sleeve 96 , the connecting sleeve 96 is used to pass the first steel rope 7 or the second steel rope 8 , and the third connecting rod 95 is provided with a micro vibration motor 94 .

[0088] When the material needs to be mixed, the first steel rope 7 and the second steel rope 8 are respectively inserted into the gap between the upper chamber and the lower chamber that need to be clamped, and the micro vibration motor 94 works. The micro vibration motor drives the upper chamber to vibrate by acting on the first steel rope 7 and the second steel rope 8, thereby improving the mixing effect and shaking out the bubbles that may be generated during the liquid addition process, so as to facilitate the experimental effect.

[0089] Example 4

[0090] On the basis of the above embodiment, the second slide grooves 11 are arranged on both sides of the return plate 1, and the two second slide grooves 11 are detachably connected with the feeding structure. Fig. 9As shown, the feeding structure includes a feeding plate 12 , a second connecting rod 121 is provided at the lower end of the feeding plate 12 , the second connecting rod 121 is detachably connected to the second sliding groove 11 , and the second connecting rod 121 can slide in the second sliding groove 11 .

[0091] The feeding plate 12 is provided with a plurality of feeding holes 13, which correspond to the lower chambers of each row in the orifice plate. The feeding plate 12 can slide in the two second slide grooves 11 so that the feeding holes 13 correspond to the lower chambers in each row. Fig.10 As shown, an auxiliary component 14 is arranged in the feeding hole 13, the inner diameter of the auxiliary component 14 gradually decreases from top to bottom, and a fixing hole is arranged at the lower end of the auxiliary component.

[0092] When the device is in use, a method for clamping the upper chamber of a Transwell plate comprises the following steps:

[0093] When feeding is required, the second connecting rod 121 is placed on the second chute 11. Fig.11 As shown, the feeding plate 12 moves to the top of the corresponding upper chamber, and the feeding holes 13 on the feeding plate 12 correspond to the upper chambers in the row of lower chambers one by one. When adding materials, the operator inserts the lower end of the feeding pipe into the fixing hole, and the fixing hole preliminarily fixes the feeding pipe to complete the feeding;

[0094] Place the return plate 1 above the orifice plate, and adjust the position of the clamping plate 3 in the first slide groove 2 so that the first steel rope 7 and the second steel rope 8 are located above the upper chamber to be clamped;

[0095] The heights of the first steel rope 7 and the second steel rope 8 are adjusted by the telescopic assembly so that the first steel rope 7 and the second steel rope 8 are located above the lower chamber corresponding to the upper chamber to be clamped;

[0096] The adjusting component shortens the gap between the first steel rope 7 and the second steel rope 8 so that the first steel rope 7 and the second steel rope 8 are located at the gap between the corresponding upper chamber and the lower chamber; when the material needs to be mixed, the micro vibration motor works, and the micro vibration motor drives the upper chamber to vibrate by acting on the first steel rope 7 and the second steel rope 8.

[0097] The height of the first steel rope 7 and the second steel rope 8 are adjusted by the telescopic assembly so that the first steel rope 7 and the second steel rope 8 take out the corresponding upper chamber;

[0098] Adjust the position of the clamping plate 3 in the first slide groove 2 so that the first steel rope 7 and the second steel rope 8 are located above the lower chamber where the upper chamber is placed;

[0099] The heights of the first steel rope 7 and the second steel rope 8 are adjusted by the telescopic assembly so that the taken-out upper chamber is located in the corresponding lower chamber.

[0100] The terms "first", "second", "third", etc. used herein are only used to distinguish the corresponding components for the sake of clarity of description, and are not intended to limit any order or emphasize importance, etc. In addition, the term "connected" used herein, unless otherwise specified, may refer to direct connection or indirect connection via other components.

[0101] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A clamping device for the upper chamber of a Transwell plate, characterized in that: It comprises a return plate (1) located above the orifice plate, first slide grooves (2) are arranged on both sides of the return plate (1), and clamping structures are arranged on the two first slide grooves (2); The clamping structure comprises two clamping plates (3) respectively located on two first slide grooves (2); a first steel rope (7) and a second steel rope (8) are arranged between the two clamping plates (3); the clamping plates (3) are capable of sliding on the corresponding first slide grooves (2) so that the first steel rope (7) and the second steel rope (8) are located above each row of upper chambers in the orifice plate; a telescopic component is arranged on the clamping plate (3), and the telescopic component is used to adjust the height of the first steel rope (7) and the second steel rope (8); The two clamping plates (3) are each provided with an adjustment component, and the adjustment component is used to adjust the distance between the first steel rope (7) and the second steel rope (8), so that the first steel rope (7) and the second steel rope (8) clamp the upper chamber of each row in the orifice plate; the adjustment component comprises a third slide groove (4) and two adjustment wheels (6) arranged on the two clamping plates (3), and the two adjustment wheels (6) are located at the upper and lower ends of the clamping plates (3); the third slide groove (4) is each provided with two action wheels (5), and the action wheel (5) is provided with an adjustment member (53), and the adjustment member (53) can adjust the position of the action wheel (5) on the corresponding third slide groove (4); the action wheels (5) on the two third slide grooves (4) are both located between the adjustment wheels (6) on the two clamping plates (3); The two ends of the first steel rope (7) are respectively connected to two adjusting wheels (6) located at the upper end of the clamping plate (3), and the two action wheels (5) at the upper ends of the two third slide grooves (4) act on the first steel rope (7), so that the first steel rope (7) includes two vertical sections and one horizontal section; The two ends of the second steel rope (8) are respectively connected to two adjusting wheels (6) located at the lower end of the clamping plate (3), and the two action wheels (5) at the lower ends of the two third slide grooves (4) act on the second steel rope (8), so that the second steel rope (8) includes two vertical sections and one horizontal section; Four action wheels (5) move on the third slide groove (4) to adjust the distance between the first steel rope (7) and the second steel rope (8) above the upper chamber of the orifice plate; Two auxiliary wheels (9) are arranged on the two third slide grooves (4), and the two auxiliary wheels (9) are connected to the clamping plate (3) via a spring rod (93). The two auxiliary wheels (9) on the third slide groove (4) are in contact with the first steel rope (7) and the second steel rope (8) respectively. A third connecting rod (95) is arranged on the auxiliary wheel (9), and a connecting sleeve (96) is arranged on the third connecting rod (95). The connecting sleeve (96) is used to pass the first steel rope (7) or the second steel rope (8). A micro vibration motor (94) is arranged on the third connecting rod (95).

2. A clamping device for the upper chamber of a Transwell plate according to claim 1, characterized in that: The adjusting wheel (6) is provided with a torsion spring, which acts on the adjusting wheel (6) to keep the first steel rope (7) and the second steel rope (8) in a straight state.

3. A clamping device for a Transwell plate upper chamber according to claim 1, characterized in that: A first connecting groove (51) is provided on the side of the action wheel (5); when the action wheel (5) acts on the first steel rope (7) or the second steel rope (8), the first steel rope (7) or the second steel rope (8) is located in the first connecting groove (51).

4. A clamping device for a Transwell plate upper chamber according to claim 1, characterized in that: Second slide grooves (11) are provided on both sides of the return plate (1), and a feeding structure is detachably connected to the two second slide grooves (11), the feeding structure comprising a feeding plate (12), and a plurality of feeding holes (13) are provided on the feeding plate (12), and the plurality of feeding holes (13) correspond one-to-one to the lower chambers of each row in the orifice plate, and the feeding plate (12) can slide in the two second slide grooves (11) so that the feeding holes (13) correspond to the lower chambers in each row respectively.

5. A clamping device for the upper chamber of a Transwell plate according to claim 4, characterized in that: An auxiliary component (14) is arranged in the feeding hole (13), the inner diameter of the auxiliary component (14) gradually decreases from top to bottom, and a fixing hole is arranged at the lower end of the auxiliary component (14).

6. A clamping device for a Transwell plate upper chamber according to claim 1, characterized in that: A partition plate (32) is provided at the lower end of the clamping plate (3), a first telescopic rod (31) is provided between the partition plate (32) and the clamping plate (3), a first connecting rod (33) is provided at the lower end of the partition plate (32), the first connecting rod (33) is located in the first sliding groove (2), and the first connecting rod (33) can slide in the first sliding groove (2); the telescopic component is a second telescopic rod provided between the partition plate (32) and the clamping plate (3) or a lifting ring (10) provided on the side of the clamping plate (3).

7. A method for clamping the upper chamber of a Transwell plate, characterized in that: The clamping device according to any one of claims 1 to 6 comprises the following steps: Place the return plate (1) above the orifice plate, and adjust the position of the clamping plate (3) in the first slide groove (2) so that the first steel rope (7) and the second steel rope (8) are located above the upper chamber to be clamped; The height of the first steel rope (7) and the second steel rope (8) are adjusted by means of a telescopic assembly so that the first steel rope (7) and the second steel rope (8) are located above the lower chamber corresponding to the upper chamber to be clamped; The adjustment component shortens the gap between the first steel rope (7) and the second steel rope (8) so that the first steel rope (7) and the second steel rope (8) are located at the gap between the corresponding upper chamber and lower chamber; The height of the first steel rope (7) and the second steel rope (8) are adjusted by means of a telescopic assembly so that the first steel rope (7) and the second steel rope (8) take out the corresponding upper chamber; Adjusting the position of the clamping plate (3) in the first slide groove (2) so that the first steel rope (7) and the second steel rope (8) are located above the lower chamber where the upper chamber is placed; The height of the first steel rope (7) and the second steel rope (8) is adjusted by means of a telescopic assembly so that the taken-out upper chamber is located in the corresponding lower chamber.

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