Preparation method and application of a fermented cistanche deserticola product for enhancing kidney tonifying, essence nourishing, hair strengthening and hair growth

Abstract

The present invention belongs to the technical field of microbial fermentation, and specifically relates to a preparation method and application of a fermented cistanche deserticola product for enhancing kidney-tonifying and essence-nourishing, as well as promoting hair growth and preventing hair loss. The steps of the preparation method are as follows: (1) Steam the dried cistanche deserticola powder or slices until cooked to obtain cistanche deserticola solids, add inulin thereto, and mix evenly to obtain a solid fermentation substrate; (2) Add a fermentation microbial community to the solid fermentation substrate, and ferment for 5 - 10 days under an anaerobic environment, with a relative humidity of 75 - 85% and a temperature of 25 - 35°C to obtain a primary fermented product; (3) Supplement inulin to the primary fermented product, and ferment for 5 - 7 days under an anaerobic environment, with a relative humidity of 75 - 85% and a temperature of 25 - 35°C to obtain a secondary fermented product; (4) Repeat the operation steps of step (3) for the secondary fermented product to obtain a fermented cistanche deserticola product. The present invention ferments cistanche deserticola by the microbial fermentation method, reduces the harmful components in cistanche deserticola, increases the content of active substances, and the prepared fermented cistanche deserticola product is non-toxic and has good effects of enhancing kidney-tonifying and essence-nourishing, as well as promoting hair growth and preventing hair loss.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial fermentation, and specifically relates to a preparation method and application of a fermented cistanche deserticola product for enhancing kidney-tonifying and sperm-nourishing, as well as hair growth and hair strengthening. Background Art

[0002] Cistanche deserticola is a very expensive traditional Chinese medicine. In addition to ginseng and pilose antler, cistanche deserticola is also an excellent tonic and has the reputation of "desert ginseng". Modern pharmacological studies on cistanche deserticola show that cistanche deserticola can stimulate the pituitary-adrenal cortex hormone, improve immune function, enhance the phagocytic ability of mononuclear macrophages, has the effect of tonifying the kidney and assisting yang, can resist cold, hypoxia and fatigue. Cistanche deserticola also has the effect of regulating endocrine and plays a certain role in protecting kidney function. In addition, cistanche deserticola can also play a certain role in nourishing hair and has the effect of promoting hair growth and strengthening hair. Patent CN 118058458 A discloses a preparation method of cistanche deserticola enzyme. In this invention, Lactobacillus reuteri, Pediococcus pentosaceus, Streptococcus thermophilus, Bifidobacterium animalis, Lactobacillus acidophilus and Lactobacillus casei are prepared into a bacterial suspension with medicinal materials. Then, sliced cistanche deserticola is stirred evenly with yellow rice wine and set aside. A cistanche deserticola enzymolysis solution is prepared, and then a laxative material is added. The bacterial suspension is inoculated into the cistanche deserticola enzymolysis solution according to the volume ratio inoculation amount for fermentation, and stored at low temperature to obtain the finished product of cistanche deserticola enzyme. This enzyme has the effects of clearing heat and detoxifying, resolving phlegm and relieving cough, and laxative, but does not conduct research on male physical health. Patent CN 116121088 A discloses a Saccharomyces cerevisiae and its application. This patent discloses a Saccharomyces cerevisiae suitable for fermenting cistanche deserticola. After fermenting cistanche deserticola with this Saccharomyces cerevisiae, the release or formation of harmful components in cistanche deserticola can be reduced. The fermented cistanche deserticola filtrate obtained by fermentation has lower cytotoxicity and higher safety, and can endow skin care products with superior anti-inflammatory, antioxidant, and enhanced non-specific immune performance of the skin and other advantages. However, the cistanche deserticola fermented filtrate obtained in this patent is applied to skin care products, and there is no data showing the oral safety of the cistanche deserticola fermented product and its effect on male physical health.

[0003] Based on the problems in the prior art, it is of great significance to develop a substance based on cistanche deserticola that can improve male physical health for the happy life of modern people. Summary of the Invention

[0004] In view of the deficiencies in the prior art, the present invention provides a preparation method and application of a fermented cistanche deserticola product for enhancing kidney-tonifying and sperm-nourishing, as well as hair growth and hair strengthening. Through the method of microbial green fermentation, cistanche deserticola is fermented to reduce the harmful components in cistanche deserticola and increase the content of active substances. The prepared fermented cistanche deserticola product is non-toxic and has good effects of tonifying the kidney and nourishing sperm, promoting hair growth and strengthening hair.

[0005] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0006] In the first aspect of the present invention, a preparation method of a fermented cistanche deserticola for enhancing kidney-tonifying and essence-nourishing and promoting hair growth and preventing hair loss is provided, including the following preparation steps:

[0007] (1) Steaming the dried cistanche deserticola powder or slices until cooked to obtain cistanche deserticola solid, adding inulin thereto, and mixing evenly to obtain a solid fermentation substrate;

[0008] (2) Adding a fermentation flora to the solid fermentation substrate in step (1), and fermenting for 5 - 10 days under an anaerobic environment, relative humidity of 75 - 85%, and a temperature of 25 - 35°C to obtain a primary fermented product;

[0009] (3) Supplementing inulin to the primary fermented product in step (2), and fermenting for 5 - 7 days under an anaerobic environment, relative humidity of 75 - 85%, and a temperature of 25 - 35°C to obtain a secondary fermented product;

[0010] (4) Repeating the operation steps of step (3) for the secondary fermented product to obtain a fermented cistanche deserticola.

[0011] In some embodiments, the cistanche deserticola bulbs are pulverized to 20 - 80 meshes.

[0012] Preferably, the cistanche deserticola bulbs are pulverized to 40 - 60 meshes.

[0013] In some embodiments, the thickness of the cistanche deserticola bulb slices is 0.5 - 1.5 cm.

[0014] In some embodiments, the total mass proportion of glucose, fructose, and sucrose in the inulin is < 14.0 wt%.

[0015] In some embodiments, the addition amount of inulin in steps (2), (3), and (4) is 1 - 2 wt% of the cistanche deserticola powder.

[0016] In some embodiments, the fermentation flora includes Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565.

[0017] In some embodiments, the mass ratio of Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 in the fermentation flora is (1 - 3):(1 - 2):1.

[0018] In some embodiments, the addition amount of the fermentation flora is 1 - 4 wt% of the solid fermentation substrate.

[0019] In the second aspect of the present invention, a fermented cistanche deserticola prepared by the preparation method described above is provided.

[0020] The third aspect of the present invention provides the application of the above-mentioned fermented cistanche in the fields of drugs or health foods.

[0021] The present invention selects the microbial fermentation method to ferment cistanche, which can reduce the toxic substances in cistanche, release active ingredients, and significantly improve the comprehensive effect of the fermented product. In this application, Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 are selected to form a fermentation flora in a certain proportion. Among them, Lactobacillus rhamnosus ATCC 53103 and Bifidobacterium adolescentis CICC 6070 belong to probiotics, and Saccharomyces cerevisiae CICC 1565 is a fungus with high biosafety. The fermentation flora is composed of bacteria and fungi, and there is no antagonism between the strains, which can form a relatively stable microbial community, promote each other, and has an excellent fermentation effect on cistanche.

[0022] The present invention ferments cistanche three times, and adjusts the fermentation conditions (microbial inoculum amount, inulin required for fermentation, temperature, and fermentation duration) to the optimal state during each fermentation process to improve the fermentation effect. In the present invention, Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 are first activated and cultured. The separated bacterial cells are directly freeze-dried. There are some nutrients in the culture medium in the bacterial cells, such as sugars, proteins, and inorganic salts, which can protect the strains and improve the freeze-dried survival rate of the strains. Then the obtained freeze-dried bacterial cells are mixed according to the mass ratio for fermentation. During the whole fermentation process, the first fermentation process is the adaptation stage of the fermentation flora to the fermentation environment, especially the components of cistanche. At this time, the microorganisms in the fermentation flora mainly adapt to the living environment by adjusting their own physiological and metabolic activities, but the effect of the first fermentation is not ideal enough. The present invention re-ferments the product of the first fermentation. By supplementing inulin to the first fermentation product, adjusting the optimal growth temperature and fermentation duration, it can enable Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 in the fermentation flora to jointly use the components contained in cistanche as nutrients for growth, reproduction, and metabolism during the second and third fermentation processes. During this process, functional enzymes such as cellulase, protease, pectinase, hemicellulase, and amylase may also be secreted, lysing the cells of cistanche, facilitating the release of the effective medicinal components to the outside, and improving the utilization rate of the effective components. In addition, during the process of using the components of cistanche as nutrients for physiological activities by the fermentation strains, the toxicity of harmful substances will also be reduced to varying degrees, or the toxic substances will be decomposed and converted into non-toxic substances, reducing the toxicity of the fermented cistanche and improving its safety.

[0023] Meanwhile, Lactobacillus rhamnosus ATCC 53103 and Bifidobacterium adolescentis CICC 6070 both belong to probiotics, and they also secrete substances beneficial to the body such as polysaccharides and proteins during the fermentation and metabolism process, which can increase the content of beneficial substances in the fermented product of Cistanche deserticola; and they can decompose macromolecular substances in Cistanche deserticola that are not absorbed by the body into small molecular active ingredients that are easily absorbed, further improving the resource utilization rate of Cistanche deserticola.

[0024] There are some probiotics that cannot utilize inulin to improve their own physiological activities. However, the applicant found that the three probiotics selected in the present invention can quickly grow and metabolize using inulin as a nutrient after activation culture and freeze-drying. Compared with the condition without adding inulin, adding inulin can enable the strains to adapt to the fermentation environment faster and construct a microbial community to jointly transform Cistanche deserticola, showing excellent fermentation effects.

[0025] Based on the problems existing in the prior art, the beneficial effects of the present invention are as follows:

[0026] 1. The present invention selects the microbial fermentation method to ferment Cistanche deserticola, which can reduce the toxic substances in Cistanche deserticola, release active ingredients, and significantly improve the comprehensive effect of the fermented product of Cistanche deserticola and the resource utilization rate of Cistanche deserticola.

[0027] 2. The present invention selects Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 to form a fermentation flora in a certain proportion. This fermentation flora consists of bacteria and fungi, and the two can form a relatively stable biological community, promoting each other, converting macromolecules in Cistanche deserticola into small molecules, and secreting beneficial polysaccharides, proteins and other substances, increasing the content of effective active ingredients in the fermented product, and having excellent fermentation effects on Cistanche deserticola.

[0028] 3. In the present invention, prebiotic inulin is added, and the microorganisms in the mixed flora can use part of the inulin as a nutrient source to improve their own metabolism, improve the microbial activity and the fermentation effect on Cistanche deserticola. Detailed Embodiments

[0029] Now, various exemplary embodiments of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and implementation schemes of the present invention. It should be understood that the terms described in the present invention are only for describing specific implementation modes and are not used to limit the present invention. In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range and each smaller range between any other stated value or intermediate value within the stated range are also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0030] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although this invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of this invention. Without departing from the scope or spirit of this invention, various improvements and changes can be made to the specific embodiments of the description of this invention, which will be obvious to those skilled in the art. Other embodiments obtained from the description of this invention will be obvious to those skilled in the art. The description and examples of this application are merely exemplary.

[0031] Regarding "comprising", "including", "having", "containing", etc. used herein, they are all open-ended terms, meaning including but not limited to.

[0032] Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 used in this invention were purchased from Biofeng, China Center for Type Culture Collection, and Testo Biotechnology respectively; the yeast extract powder used was purchased from Shanghai Macklin; the cistanche used was Cistanche tubulosa, cut into slices with a thickness of 1 cm; the inulin used was Welove Inulin (the total mass ratio of glucose, fructose, and sucrose in inulin < 14.0 wt%), purchased from Shenzhen Tuojian Biotechnology Co., Ltd.

[0033] Preparation Example 1

[0034] Preparation of Lactobacillus rhamnosus ATCC 53103:

[0035] After melting the glycerol tube storing Lactobacillus rhamnosus ATCC 53103 in an anaerobic incubator, 1 g of the melted liquid was taken with a sterile syringe and added to 100 mL of PYG medium, and cultured in an anaerobic incubator at 37 °C and 150 r / min until the logarithmic growth phase, then centrifuged at 6000 r / min to obtain the bacterial cells, and freeze-dried to obtain Lactobacillus rhamnosus ATCC 53103.

[0036] Preparation Example 2

[0037] Preparation of Bifidobacterium adolescentis CICC 6070:

[0038] After thawing the glycerol tube containing Bifidobacterium adolescentis CICC 6070 in an anaerobic incubator, 1 g of the thawed liquid was taken with a sterile syringe and added to 100 mL of MRS broth medium. The mixture was cultured in the anaerobic incubator at 37 °C and 150 r / min until the logarithmic growth phase, and then centrifuged at 6000 r / min to obtain the bacterial cells, which were freeze-dried to obtain Bifidobacterium adolescentis CICC 6070.

[0039] Preparation Example 3

[0040] Preparation of Saccharomyces cerevisiae CICC 1565:

[0041] After thawing the glycerol tube containing Saccharomyces cerevisiae CICC 1565 in an anaerobic incubator, 1 g of the thawed liquid was taken with a sterile syringe and added to 100 mL of the medium. The mixture was cultured in an aerobic environment at 28 °C and 150 r / min until the logarithmic growth phase, and then centrifuged at 6000 r / min to obtain the bacterial cells, which were freeze-dried to obtain Saccharomyces cerevisiae CICC 1565.

[0042] The PYG medium and MRS broth medium used in Preparation Examples 1 and 2 were both purchased from Qingdao Haibo Biotech;

[0043] The medium used in Preparation Example 3 was obtained by dissolving 10 g of yeast extract powder, 20 g of glucose, and 20 g of peptone in 1 L of pure water, adjusting the pH to 5, sterilizing, and cooling.

[0044] Preparation Example 4

[0045] Preparation of the fermentation flora:

[0046] Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 obtained in Preparation Examples 1 - 3 were mixed evenly at a mass ratio of 2:1.5:1 to obtain the fermentation flora.

[0047] Preparation Example 5

[0048] Preparation of the fermentation flora:

[0049] Lactobacillus rhamnosus ATCC 53103 and Bifidobacterium adolescentis CICC 6070 obtained in Preparation Examples 1 and 2 were mixed evenly at a mass ratio of 2:1.5 to obtain the fermentation flora.

[0050] Preparation Example 6

[0051] Preparation of the fermentation flora:

[0052] Mix the Bifidobacterium adolescentis CICC 6070 and Saccharomyces cerevisiae CICC 1565 obtained in Preparation Examples 2 and 3 in a mass ratio of 1.5:1 and mix them evenly to obtain a fermentation flora.

[0053] Preparation Example 7

[0054] Preparation of the fermentation flora:

[0055] Mix the Lactobacillus rhamnosus ATCC 53103 and Saccharomyces cerevisiae CICC 1565 obtained in Preparation Examples 1 and 3 in a mass ratio of 2:1 and mix them evenly to obtain a fermentation flora.

[0056] Example 1

[0057] A preparation method of a fermented product of Cistanche deserticola for enhancing kidney-tonifying and essence-nourishing and promoting hair growth and preventing hair loss, comprising the following preparation steps:

[0058] (1) Slice and steam the dried Cistanche deserticola to obtain a Cistanche deserticola solid, add 1 wt% of inulin thereto, and mix evenly to obtain a solid fermentation substrate;

[0059] (2) Add 1 wt% of the fermentation flora to the solid fermentation substrate in step (1), and ferment at 25 °C under an anaerobic environment, a relative humidity of 75% for 10 days to obtain a primary fermented product;

[0060] (3) Supplement 1 wt% of inulin to the primary fermented product in step (2), and ferment at 25 °C under an anaerobic environment, a relative humidity of 75% for 7 days to obtain a secondary fermented product;

[0061] (4) Repeat the operation steps of step (3) for the secondary fermented product to obtain a fermented product of Cistanche deserticola.

[0062] The fermentation flora used is obtained from Preparation Example 4.

[0063] Example 2

[0064] A preparation method of a fermented product of Cistanche deserticola for enhancing kidney-tonifying and essence-nourishing and promoting hair growth and preventing hair loss, comprising the following preparation steps:

[0065] (1) Slice and steam the dried Cistanche deserticola to obtain a Cistanche deserticola solid, add 4 wt% of inulin thereto, and mix evenly to obtain a solid fermentation substrate;

[0066] (2) Add 4 wt% of the fermentation flora to the solid fermentation substrate in step (1), and ferment at 35 °C under an anaerobic environment, a relative humidity of 85% for 5 days to obtain a primary fermented product;

[0067] (3) Supplement 2 wt% of inulin to the primary fermented product in step (2), and ferment for 5 days at 35°C with an anaerobic environment and a relative humidity of 85% to obtain a secondary fermented product;

[0068] (4) Repeat the operation steps of step (3) for the secondary fermented product to obtain a fermented cistanche deserticola product.

[0069] The fermentation flora used is obtained from Preparation Example 4.

[0070] Example 3

[0071] A preparation method of a fermented cistanche deserticola product for enhancing kidney-tonifying and essence-nourishing and preventing hair loss and promoting hair growth, comprising the following preparation steps:

[0072] (1) Steam and cook the dried cistanche deserticola slices to obtain cistanche deserticola solids, add 1.5 wt% of inulin thereto, and mix evenly to obtain a solid fermentation substrate;

[0073] (2) Add 2.5 wt% of the fermentation flora to the solid fermentation substrate in step (1), and ferment for 8 days at 30°C with an anaerobic environment and a relative humidity of 80% to obtain a primary fermented product;

[0074] (3) Supplement 1.5 wt% of inulin to the primary fermented product in step (2), and ferment for 6 days at 30°C with an anaerobic environment and a relative humidity of 80% to obtain a secondary fermented product;

[0075] (4) Repeat the operation steps of step (3) for the secondary fermented product to obtain a fermented cistanche deserticola product.

[0076] The fermentation flora used is obtained from Preparation Example 4.

[0077] Example 4

[0078] A preparation method of a fermented cistanche deserticola product for enhancing kidney-tonifying and essence-nourishing and preventing hair loss and promoting hair growth, the specific implementation manner is the same as that of Example 3, except that the fermentation flora used is obtained from Preparation Example 5.

[0079] Example 5

[0080] A preparation method of a fermented cistanche deserticola product for enhancing kidney-tonifying and essence-nourishing and preventing hair loss and promoting hair growth, the specific implementation manner is the same as that of Example 3, except that the fermentation flora used is obtained from Preparation Example 6.

[0081] Example 6 <x

[0082] A preparation method of a fermented cistanche deserticola product for enhancing kidney-tonifying and essence-nourishing and preventing hair loss and promoting hair growth, the specific implementation manner is the same as that of Example 3, except that the fermentation flora used is obtained from Preparation Example 7.

[0083] Example 7

[0084] A preparation method of a fermented cistanche deserticola product for enhancing kidney tonifying, essence nourishing, hair strengthening and hair growth, comprising the following preparation steps:

[0085] (1) Slicing and steaming dried cistanche deserticola to obtain cistanche deserticola solid, adding 1.5 wt% inulin thereto, and mixing evenly to obtain a solid fermentation substrate;

[0086] (2) Adding 5 wt% of a fermentation flora to the solid fermentation substrate in step (1), and fermenting at 30 °C with 80% relative humidity in an anaerobic environment for 8 days to obtain a primary fermentation product;

[0087] (3) Supplementing 1.5 wt% of inulin to the primary fermentation product in step (2), and fermenting at 30 °C with 80% relative humidity in an anaerobic environment for 6 days to obtain a secondary fermentation product;

[0088] (4) Repeating the operation steps of step (3) for the secondary fermentation product to obtain a fermented cistanche deserticola product.

[0089] The fermentation flora used is obtained from Preparation Example 4.

[0090] Comparative Example 1

[0091] A preparation method of a fermented cistanche deserticola product for enhancing kidney tonifying, essence nourishing, hair strengthening and hair growth, the specific implementation manner is the same as that of Example 3, except that Lactobacillus rhamnosus ATCC 53103 obtained from Preparation Example 1 with the same mass is used to replace the fermentation flora.

[0092] Comparative Example 2

[0093] A preparation method of a fermented cistanche deserticola product for enhancing kidney tonifying, essence nourishing, hair strengthening and hair growth, the specific implementation manner is the same as that of Example 3, except that Bifidobacterium adolescentis CICC 6070 obtained from Preparation Example 2 with the same mass is used to replace the fermentation flora.

[0094] Comparative Example 3

[0095] A preparation method of a fermented cistanche deserticola product for enhancing kidney tonifying, essence nourishing, hair strengthening and hair growth, the specific implementation manner is the same as that of Example 3, except that Saccharomyces cerevisiae CICC 1565 obtained from Preparation Example 3 with the same mass is used to replace the fermentation flora.

[0096] Comparative Example 4

[0097] A preparation method of a fermented cistanche deserticola product for enhancing kidney tonifying, essence nourishing, hair strengthening and hair growth, comprising the following preparation steps:

[0098] (1) Slicing and steaming dried cistanche deserticola to obtain cistanche deserticola solid;

[0099] (2) Add 2.5 wt% of the fermentation flora to the prepared Cistanche deserticola solid in step (1), and ferment it for 8 days under an anaerobic environment, relative humidity of 80%, and a temperature of 30 °C to obtain the primary fermentation product.

[0100] (3) Ferment the primary fermentation product in step (2) for 6 days under an anaerobic environment, relative humidity of 80%, and a temperature of 30 °C to obtain the secondary fermentation product.

[0101] (4) Repeat the operation steps of step (3) for the secondary fermentation product to obtain the Cistanche deserticola fermentation product.

[0102] The fermentation flora used is obtained from Preparation Example 4.

[0103] Performance test of the Cistanche deserticola fermentation product

[0104] Conduct the following performance tests on the Cistanche deserticola fermentation products obtained in each example and comparative example:

[0105] 1. Cytotoxicity evaluation experiment

[0106] Take 10 g of the Cistanche deserticola fermentation products obtained in each example and comparative example, add 10 g of deionized water, ultrasonically extract for 15 min at 40 KHz, then centrifuge at 5000 rpm / min for 10 min, and filter to remove solid residues and bacteria to obtain the test extraction solution. Use the CCK8 kit and WST-1 method purchased from Shenzhen Ampere Biotechnology Co., Ltd. to conduct the cytotoxicity evaluation experiment.

[0107] When the relative growth rate (RGR) is lower than 70%, it is considered to have cytotoxicity. The test results of the cytotoxicity (RGR%) of different samples are shown in Table 1.

[0108] Table 1

[0109]

[0110] As can be seen from Table 1, the extract of the fermented product of Cistanche deserticola prepared in Examples 1-3 is not toxic to cells when the content of the test solution is 0.04%-50%, which indirectly shows that the fermented product of Cistanche deserticola prepared by the present invention is not toxic and has high safety; in Examples 4-6, the microbial composition of the fermentation flora used changes, and in Comparative Examples 1-3, a single strain is used instead of a mixed strain. Since the physiological functions of the two strains in combination or a single strain are limited, their fermentation effect on Cistanche deserticola is worse than that of the mixed flora composed of three strains, and the toxic substances contained in Cistanche deserticola are not removed ideally, resulting in a significant increase in the toxicity of the fermented product of Cistanche deserticola; in Example 7, the addition amount of nutrients is changed, resulting in a decrease in the biological utilization rate of Cistanche deserticola by the mixed flora in an environment of excessive nutrients, a poor fermentation effect, and an increase in toxicity; in Comparative Example 4, inulin is not added during the fermentation process, resulting in a decrease in the activity of the three strains in the mixed flora, a poor fermentation effect on Cistanche deserticola, and an increase in toxicity.

[0111] Based on the data of the cell toxicity evaluation experiment, the fermented product of Cistanche deserticola prepared in Example 3 was selected for the following experiment.

[0112] 2. Experiment on the effect of tonifying the kidney and replenishing essence

[0113] Experiment 1: Thirty adult male mice weighing 25 g - 30 g were randomly divided into groups 1, 2, and 3, with 10 mice in each group. Twenty mice in groups 2 and 3 were anesthetized with ether and their bilateral testes were removed. On the 7th day after the operation, the fermented product of Cistanche deserticola obtained in Example 3 was put into the feed of each mouse in group 2 at an addition amount of 1 g / day, administered once a day, and continuously administered for two weeks; groups 1 and 3 were fed with normal feed; 24 hours after the last administration, 10 mice in groups 1-3 were sacrificed respectively, and the seminal vesicles - prostate and preputial glands were removed and weighed, and the test results were averaged. The specific test results are shown in Table 2.

[0114] Experiment 2: Thirty adult male mice weighing 25 g - 30 g were randomly divided into groups 4, 5, and 6, with 10 mice in each group. The fermented product of Cistanche deserticola obtained in Example 3 was put into the feed of each mouse in group 4 at an addition amount of 1 g / day, administered once a day, and continuously administered for two weeks; group 5 was fed with normal feed for two weeks; unfermented Cistanche deserticola was put into the feed of each mouse in group 6 at an addition amount of 1 g / day, administered once a day, and continuously administered for two weeks. Ten mice in groups 4, 5, and 6 were sacrificed 30 minutes after the last administration, and the bilateral epididymides and testes were removed and weighed. Immediately, the epididymides were minced and put into a test tube containing 0.2 mL of physiological saline, placed in a water bath at 34°C for 10 minutes, 0.1 mL was taken out, diluted 20 times with 5 wt% NaHCO3 solution, and the sperm count was measured on a counting plate, and the test results were averaged. The specific test results are shown in Table 3.

[0115] Table 2

[0116]

[0117] Table 3

[0118]

[0119] As can be seen from Tables 2 and 3, by comparing the test data of the three experimental groups, it can be seen that the fermented product of Cistanche deserticola prepared in Example 3 can significantly increase the weights of the seminal vesicle-prostate, preputial gland, testis, epididymis and sperm count of castrated mice. Thus, it can be seen that the fermented product of Cistanche deserticola obtained in Example 3 of the present invention has good effects of invigorating yang and tonifying the kidney and replenishing essence.

[0120] The above are only the preferred embodiments of the present invention, and do not impose any form of limitation on this application. Although this application is disclosed as above with preferred embodiments, it is not intended to limit this application. Any person skilled in the art, without departing from the scope of the technical solution of this application, making some changes or modifications using the technical content disclosed above is equivalent to equivalent implementation cases. However, as long as it does not depart from the content of the technical solution of the present invention, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention still fall within the scope of the technical solution.

Claims

1. A preparation method of a fermented cistanche deserticola for enhancing kidney tonifying, essence nourishing, hair strengthening and hair growth, characterized in that, It includes the following preparation steps: (1) Steam the dried cistanche powder or slices until cooked to obtain cistanche solid, add inulin thereto, and mix evenly to obtain a solid fermentation substrate; (2) Add a fermentation flora to the solid fermentation substrate in step (1), and ferment for 5 - 10 days under an anaerobic environment, relative humidity of 75 - 85%, and a temperature of 25 - 35°C to obtain a primary fermentation product; (3) Supplement inulin to the primary fermentation product in step (2), and ferment for 5 - 7 days under an anaerobic environment, relative humidity of 75 - 85%, and a temperature of 25 - 35°C to obtain a secondary fermentation product; (4) Repeat the operation steps of step (3) for the secondary fermentation product to obtain a cistanche fermentation product; The total mass ratio of glucose, fructose, and sucrose in the inulin is <14.0 wt%; The addition amount of inulin in steps (2), (3), and (4) is 1 - 2 wt% of the cistanche powder; The fermentation flora includes Lactobacillus rhamnosus ATCC 53103, Bifidobacterium adolescentis CICC 6070, and Saccharomyces cerevisiae CICC 1565 with a mass ratio of (1 - 3):(1 - 2):1; The addition amount of the fermentation flora is 1 - 4 wt% of the solid fermentation substrate.

2. The preparation method according to claim 1, characterized in that, The cistanche bulb is pulverized to 20 - 80 meshes.

3. The preparation method according to claim 1, wherein The thickness of the sliced cistanche bulb is ≤2 cm.

4. A cistanche fermentation product prepared by the preparation method according to any one of claims 1 - 3.

5. An application of the cistanche fermentation product according to claim 4 in the field of preparing drugs or health foods.

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