A black hair essence composition, its preparation method and application

Abstract

The present invention discloses a black hair essence composition, a preparation method and an application thereof. The black hair essence composition comprises raw materials in the following parts by weight, and the raw materials are prepared according to the ratio: 15-20 parts of black mulberry fruits, 10-15 parts of fleece-flower roots, 10-15 parts of glossy privet fruits, 8-10 parts of salvia miltiorrhiza, 5-8 parts of angelica sinensis, 5-8 parts of ligusticum wallichii, 8-10 parts of oriental arborvitae leaves, 5-8 parts of sesame seeds, 5-8 parts of wolfberries, 5-8 parts of dried ginger and 5-8 parts of malt; The present invention is prepared by adopting the compatibility of pure natural traditional Chinese medicine ingredients and a multi-bacteria mixed fermentation process. The composition can activate the activity of tyrosinase, promote the generation of melanin, can accelerate the blood flow of scalp capillaries and strengthen the penetration of nutrient components, promote the absorption of nutrients, promote the conversion of white hair to black hair, and has excellent black hair and hair nourishing effects.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to a black hair essence composition, a preparation method thereof, and an application thereof. Background Art

[0002] With the accelerating pace of people's lives and the increasing severity of environmental pollution, more and more people are facing hair problems such as premature graying and hair loss. Although most of the current hair growth products on the market can temporarily improve hair quality, they often contain harmful chemical components to the human body, and long-term use is likely to cause side effects, or the effective components of the medicinal materials are not extracted thoroughly enough to achieve the desired effect. Therefore, it is necessary to develop a safe, effective, and side-effect-free black hair product to meet the market demand. Summary of the Invention

[0003] In view of the above technical problems, the object of the present invention is to provide a black hair essence composition, a preparation method thereof, and an application thereof. The black hair essence composition is prepared by microbial fermentation of natural traditional Chinese medicine components. This composition can activate the activity of tyrosinase, proliferate melanocytes, and at the same time provide a variety of active nutritional components for the scalp and hair, which can improve the penetration and absorption of the scalp and hair, and promote the conversion of white hair to black hair through a combination of multiple ways.

[0004] To this end, the present invention adopts the following technical solutions:

[0005] In the first aspect, the present invention provides a black hair essence composition, which comprises the following raw materials in parts by weight and is prepared according to the raw material ratio: 15-20 parts of black mulberry fruits, 10-15 parts of fleece-flower roots, 10-15 parts of glossy privet fruits, 8-10 parts of danshen roots, 5-8 parts of angelica roots, 5-8 parts of chuanxiong rhizomes, 8-10 parts of oriental arborvitae leaves, 5-8 parts of sesame seeds, 5-8 parts of wolfberries, 5-8 parts of dried ginger, and 5-8 parts of malt.

[0006] Preferably, it comprises the following raw materials in parts by weight and is prepared according to the raw material ratio: 20 parts of black mulberry fruits, 15 parts of fleece-flower roots, 15 parts of glossy privet fruits, 10 parts of danshen roots, 5 parts of angelica roots, 5 parts of chuanxiong rhizomes, 10 parts of oriental arborvitae leaves, 5 parts of sesame seeds, 5 parts of wolfberries, 5 parts of dried ginger, and 5 parts of malt.

[0007] Preferably, it further comprises one or more of costus roots, quisqualis fruits, picrorhiza rhizomes, and smoke trees.

[0008] Preferably, it comprises the following raw materials in parts by weight and is prepared according to the raw material ratio: 15-20 parts of black mulberry fruits, 10-15 parts of fleece-flower roots, 10-15 parts of glossy privet fruits, 8-10 parts of danshen roots, 5-8 parts of angelica roots, 5-8 parts of chuanxiong rhizomes, 8-10 parts of oriental arborvitae leaves, 5-8 parts of sesame seeds, 5-8 parts of wolfberries, 5-8 parts of dried ginger, 5-8 parts of malt, 5-8 parts of costus roots, 5-8 parts of quisqualis fruits, 5-8 parts of picrorhiza rhizomes, and 5-8 parts of smoke trees.

[0009] In the above technical scheme, Inula is a herb included in the Chinese Pharmacopoeia, and its medicinal source is the dried root of Inula or Tibetan Aucklandia of the Asteraceae family. It has the effects of strengthening the spleen and stomach, regulating qi and relieving depression, relieving pain and stabilizing pregnancy. It is used for chest and flank, abdominal distension and pain, vomiting and diarrhea, chest and flank contusion, pain due to side stitches, and restless fetal movement. Inula essential oil has sedative, bactericidal, antipyretic and anthelmintic effects.

[0010] In the above technical scheme, Quisqualis is a climbing shrub of the family Combretaceae and the genus Combretaceae, 2-8 meters high, with twigs covered with short brown-yellow soft hairs. As a traditional Chinese medicine, Quisqualis is sweet and warm in nature. The medicinal value of Quisqualis is that its fruit contains medicinal ingredients that can eliminate roundworms. In addition, Quisqualis also has the effect of resisting skin fungi and has inhibitory effects on many pathogens. In the present invention, the fruit can be taken as a raw material.

[0011] In the above technical scheme, the rhizome of Picrorhizon chinensis or Picrorhizon tibetica of Scrophulariaceae is bitter and cold in nature, and enters the liver, stomach and large intestine meridians. Picrorhizon contains iridoid glycosides (picroside I, I, I, catalpol, aucubin), triterpenoid glycosides (cucurbitacin β-2-D-glucoside, picericin), etc., and has anti-inflammatory, anti-allergic, liver-protecting and choleretic, antioxidant and other effects.

[0012] In the above technical scheme, Cotinus coggygria belongs to Anacardiaceae, so Fisetin is also called Rhus flavonoids. Fisetin is a common plant pigment, which has pharmacological characteristics such as anti-oxidation, anti-inflammatory and neuroprotection, and can play an anti-tumor role by inducing tumor cell apoptosis, affecting the signal transduction pathway of tumor cells, inhibiting the proliferation of tumor cells, inhibiting the migration and invasion of tumor cells, etc. In the present invention, its leaves can be taken as raw materials.

[0013] In a second aspect, the present invention provides a method for preparing a black hair essence composition, comprising the following steps:

[0014] S1, weighing medicinal materials, crushing and mixing, adding purified water in an amount of 5-10 times the weight of the mixed medicinal material powder, adjusting the pH to 5-7, and sterilizing at high temperature to obtain a sterile culture solution;

[0015] S2, when the culture solution is cooled to 30°C, inoculate 0.5-1% of the weight of the medicinal material with lactobacillus and 0.5-1% of the yeast in a sterile environment, and ferment anaerobically at 30°C for 24-48h. After the fermentation is completed, inactivate at 121°C, and cool to room temperature after inactivation;

[0016] S3, the inactivated fermentation liquid is coarsely filtered with gauze to obtain a crude extract, the crude extract is centrifuged, and finely filtered with a ceramic membrane to obtain a refined fermentation liquid, a preservative is added to the refined fermentation liquid, sterilized at 121° C., and cooled to room temperature.

[0017] Preferably, the weight ratio of the medicinal material powder to water is 1:10.

[0018] Preferably, the Lactobacillus is selected from one or more of Streptococcus thermophilus, Lactobacillus bulgaricus, Bifidobacterium longum, Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, Bifidobacterium adolescentis, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus plantarum, and Lactobacillus casei.

[0019] Preferably, the preservatives are potassium sorbate and sodium benzoate, which are respectively five-thousandths and one percent of the weight of the fermentation broth.

[0020] In a third aspect, the present invention also provides the application of the black hair essence liquid composition of the first aspect of the present invention or the black hair essence liquid composition prepared by the preparation method of the second aspect of the present invention in the preparation of black hair products.

[0021] Preferably, the black hair products include black hair sprays, hair nutrient waters, black hair essence liquids, shampoos, conditioners, or hair dyes.

[0022] The beneficial effects of the present invention are as follows:

[0023] The black hair essence liquid composition provided by the present invention is prepared by using a natural Chinese medicine ingredient compatibility and a multi-bacteria mixed fermentation process. Through the combination of Ligusticum chuanxiong, Salvia miltiorrhiza, and Dry Ginger, the blood flow of the scalp capillaries is accelerated, and the absorption of nutrients is promoted; the combination of black mulberry fruit, Polygonum multiflorum, Platycladus orientalis, Glossy privet fruit, Lycium barbarum, and black sesame is used to activate the activity of tyrosinase and promote the production of melanin; malt is used to increase the vitamin nutrition required for hair; through mixed fermentation, ethanol with a concentration of 4-8 degrees is produced under the action of yeast, which enhances the penetration and absorption of various nutritional components. The formula of the present invention combines multiple ways to promote the conversion of white hair to black hair and has excellent black hair and hair nourishing effects.

[0024] The black hair essence liquid composition provided by the present invention may further contain Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria. When combined with other Chinese medicine ingredients, they can further act synergistically to significantly enhance the activity of tyrosinase and improve the black hair effect.

[0025] The black hair essence liquid composition provided by the present invention has all-natural Chinese medicine ingredients and does not contain any chemical components harmful to the human body, being safe and mild. The composition of the present invention is rich in nutrients and can fundamentally improve hair quality problems. It has a very ideal improvement effect especially on white hair and hair loss caused by malnutrition. Detailed implementation manners

[0026] Below by specific embodiment, the specific implementation mode of this explanation technical scheme is further described, these embodiments are for the detailed description of this technical scheme, rather than for limiting this technical scheme. Based on the embodiment in this description, all other embodiments obtained by those of ordinary skill in the art without making creative work premise belong to the scope of protection of this description. Unless otherwise specified, the reagent, method and equipment used in the present invention are conventional reagents, methods and equipment in the art, and the reagents and materials used in the following examples are all commercially available.

[0027] Example 1

[0028] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 15 parts of black mulberry fruit, 10 parts of Polygonum multiflorum, 10 parts of Ligustrum lucidum fruit, 8 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Chuanxiong, 8 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 8 parts of dried ginger and 8 parts of malt.

[0029] Example 2

[0030] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of polygonum multiflorum, 15 parts of glossy privet fruit, 10 parts of salvia miltiorrhiza, 5 parts of angelica, 5 parts of chuanxiong, 10 parts of orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger and 5 parts of malt.

[0031] Example 3

[0032] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt and 5 parts of Inula japonica.

[0033] Example 4

[0034] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt and 5 parts of Quisqualis creeper.

[0035] Example 5

[0036] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt and 5 parts of Picrorhizon.

[0037] Example 6

[0038] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Ligusticum chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt and 5 parts of Cotinus coggygria.

[0039] Example 7

[0040] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Ligusticum chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt, 5 parts of Inula japonica and 5 parts of Quisqualis chinensis.

[0041] Example 8

[0042] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Ligusticum chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt, 5 parts of Picrorhizon and 5 parts of Cotinus coggygria.

[0043] Example 9

[0044] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Ligusticum chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt, 5 parts of Inula japonica and 5 parts of Cotinus coggygria.

[0045] Example 10

[0046] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 20 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 5 parts of Ligusticum chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt, 5 parts of Inula officinalis, 5 parts of Quisqualis creeper, 5 parts of Picrorhizon and 5 parts of Cotinus coggygria.

[0047] Embodiment 11

[0048] A hair blackening essence composition comprises the following raw materials, measured by weight, and is prepared according to the raw material ratio: 15 parts of black mulberry fruit, 15 parts of Polygonum multiflorum, 15 parts of Ligustrum lucidum fruit, 10 parts of Salvia miltiorrhiza, 5 parts of Angelica sinensis, 8 parts of Ligusticum chuanxiong, 10 parts of Platycladus orientalis leaves, 5 parts of sesame, 5 parts of wolfberry, 5 parts of dried ginger, 5 parts of malt, 7 parts of Inula officinalis, 5 parts of Quisqualis creeper, 5 parts of Picrorhizon and 6 parts of Cotinus coggygria.

[0049] The preparation method of the hair - darkening essence composition provided in Examples 1 - 11 includes the following steps:

[0050] S1, Weigh the medicinal material raw materials, crush and mix them, add pure water with a volume 5 times that of the weight of the mixed medicinal material powder, adjust the pH to 6, and sterilize at 121 °C for 30 minutes to obtain a sterile culture solution;

[0051] S2, When the culture solution cools to about 30 °C, under a sterile environment, inoculate 1% Lactobacillus reuteri and 1% yeast based on the weight of the medicinal materials, ferment anaerobically at 30 °C for 48 h. After fermentation is completed, inactivate at 121 °C for 15 minutes, and cool to room temperature after inactivation;

[0052] S3, Coarsely filter the inactivated fermentation broth with gauze to obtain a crude extract. Centrifuge and precisely filter the crude extract with a ceramic membrane to obtain a refined fermentation broth. Add 0.5% potassium sorbate and 0.5% sodium benzoate to the refined fermentation broth, sterilize at 121 °C for 30 minutes, and can it after cooling to room temperature.

[0053] Comparative Example 1

[0054] Compared with Example 2, the difference lies in the different preparation methods.

[0055] The preparation method of the hair - darkening essence composition in this comparative example is: Weigh the medicinal material raw materials, crush and mix them, add distilled water with a weight of 5 times, decoct for 60 minutes, filter, and collect the filtrate to obtain it.

[0056] Comparative Example 2

[0057] Compared with Example 2, the difference is that Chuanxiong (Rhizoma Chuanxiong), Danshen (Salvia miltiorrhiza), and Ganjiang (Dry Ginger) are not added, and the others are the same.

[0058] The preparation method is the same as that of Example 2.

[0059] Comparative Example 3

[0060] Compared with Example 2, the difference is that Chuanxiong (Rhizoma Chuanxiong), Danshen (Salvia miltiorrhiza), Ganjiang (Dry Ginger), and Maiya (Hordeum vulgare L. germinatum) are not added, and the others are the same.

[0061] The preparation method is the same as that of Example 2.

[0062] Comparative Example 4

[0063] Compared with Example 2, the difference is that Chuanxiong (Rhizoma Chuanxiong), Danshen (Salvia miltiorrhiza), and Ganjiang (Dry Ginger) are not added, and the preparation method is different, and the others are the same.

[0064] The preparation method of the hair - darkening essence composition in this comparative example is: Weigh the medicinal material raw materials, crush and mix them, add distilled water with a weight of 5 times, decoct for 60 minutes, filter, and collect the filtrate to obtain it.

[0065] Comparative Example 5

[0066] Compared with Example 2, the differences are that not adding Chuanxiong rhizome, Salvia miltiorrhiza, dried ginger, and malt, and the preparation method is different, while others are the same.

[0067] The preparation method of the hair blackening essence liquid composition of this comparative example is as follows: Weigh the medicinal material raw materials, crush and mix them, add 5 times the weight part of distilled water, decoct for 60 minutes, filter, and collect the filtrate to obtain it.

[0068] Comparative Example 6

[0069] Compared with Example 2, the differences are that not adding black mulberry fruit, Glossy privet fruit, dried ginger, and malt, while others are the same.

[0070] The preparation method is the same as that of Example 2.

[0071] Perform efficacy tests on the hair blackening essence liquid compositions of Examples 1 - 11 and Comparative Examples 1 - 6:

[0072] I. Detect the tyrosinase activity

[0073] Prepare an L - dopa solution with a concentration of 1 g / L using a 0.2 mol / L phosphate buffer solution (pH 6.8). Prepare a tyrosinase solution with a concentration of 100 U / mL using a 0.2 mol / L phosphate buffer solution (pH 6.8). Prepare a test solution containing the essence liquid composition of Examples 1 - 11 or Comparative Examples 1 - 6 with a concentration of 2 mg / mL using a 0.2 mol / L phosphate buffer solution (pH 6.8).

[0074] Take 40 μL of the L - dopa solution, 40 μL of the test medicinal liquid and / or 40 μL of the tyrosinase solution and add them into a 96 - well plate, then react in a water bath at 30 °C for 40 min; after the reaction is completed, measure the absorbance at 490 nm. Each group of experiments is repeated 3 times, and the absorbance of each group of experiments takes the average value of 3 measurements to obtain the absorbance C. Referring to the above method, use the phosphate buffer solution to replace the tyrosinase solution, and the measured absorbance is D; use the phosphate buffer solution to replace the test medicinal liquid, and the measured absorbance is A; use the phosphate buffer solution to replace the test medicinal liquid and the tyrosinase solution, and the measured absorbance is B.

[0075] Tyrosinase activation rate (%) = [(C - D)-(A - B)] / (A - B)*100%

[0076] The higher the tyrosinase activation rate, the stronger the ability to promote melanin production, that is, it indicates that the hair blackening effect of the essence liquid composition is better. The detection data are shown in Table 1 below.

[0077] Table 1 Tyrosinase activation rate (%)

[0078]

[0079] As can be seen from Table 1, the black hair essence composition provided in Examples 1-11 of the present invention has a relatively good tyrosinase activation rate. Compared with Example 2, in Examples 3-6, one of Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria was added respectively, and the tyrosinase activation rate increased slightly; compared with Example 2, in Examples 7-9, any two of Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria were added respectively, and the tyrosinase activation rate increased slightly; compared with Example 2, in Examples 10-11, four raw materials of Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria were added, and the tyrosinase activation rate increased significantly. It shows that the simultaneous addition of Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria has an unexpected synergistic effect and can significantly improve the tyrosinase activity.

[0080] Compared with Example 2, in Comparative Example 1, a conventional traditional Chinese medicine extraction method was used to decoct the traditional Chinese medicine raw materials to obtain a composition, and the tyrosinase activation rate was significantly lower than that of Example 2. It shows that the composition prepared by the preparation process of the black hair essence composition provided by the present invention has a better tyrosinase activation rate.

[0081] Compared with Example 2, in Comparative Example 2, the raw materials of Ligusticum chuanxiong, Salvia miltiorrhiza, and Zingiber officinale were not added, and the tyrosinase activation rate decreased, indicating that the composition prepared with the raw materials of the present invention's formula has a better tyrosinase activation rate, and changing the raw materials in the formula reduces the tyrosinase activation rate.

[0082] Compared with Example 2, in Comparative Example 3, the raw materials of Ligusticum chuanxiong, Salvia miltiorrhiza, Zingiber officinale, and Hordeum vulgare were not added, and the tyrosinase activation rate decreased, indicating that the composition prepared with the raw materials of the present invention's formula has a better tyrosinase activation rate, and changing the raw materials in the formula reduces the tyrosinase activation rate.

[0083] Compared with Example 2, in Comparative Example 4, the raw materials of Ligusticum chuanxiong, Salvia miltiorrhiza, and Zingiber officinale were not added, and the preparation method was different, and the tyrosinase activation rate decreased, which was lower than that of Comparative Example 2. It shows that the composition prepared by the preparation process of the black hair essence composition of the present invention has a better tyrosinase activation rate.

[0084] Compared with Example 2, in Comparative Example 5, the raw materials of Ligusticum chuanxiong, Salvia miltiorrhiza, Zingiber officinale, and Hordeum vulgare were not added, and the preparation method was different, and the tyrosinase activation rate decreased, which was lower than that of Comparative Example 3. It shows that the composition prepared by the preparation process of the black hair essence composition of the present invention has a better tyrosinase activation rate.

[0085] Compared with Example 2, in Comparative Example 6, the raw materials of Morus nigra, Glossy privet fruit, Zingiber officinale, and Hordeum vulgare were not added, and the tyrosinase activation rate decreased, indicating that the composition prepared with the raw materials of the present invention's formula has a better tyrosinase activation rate, and changing the raw materials in the formula reduces the tyrosinase activation rate.

[0086] II. Black hair effect test

[0087] 170 volunteers aged 35 - 50 with white hair were selected and randomly divided into 17 groups, with 10 volunteers in each group. The essence compositions of Test Groups 1 - 11 and Comparative Examples 1 - 6 described above were used. The subjects evenly applied it to the scalp, hair roots to hair tips once in the morning and once in the evening every day, and gently massaged it to make it evenly absorbed. The dosage for each use was 5 mL, and it was rubbed for 3 minutes. After continuous use for 3 months, scores were observed and the average scores of each group were statistically calculated.

[0088] The criteria for observing and scoring are as follows: The highest score is 5 points and the lowest score is 1 point. 5 points: All the newly grown hair has turned black; 4 points: The roots of the newly grown hair turn black and the part above the roots is black; 3 points: The roots of the newly grown hair are black and the part above the roots is light black; 2 points: The roots of the newly grown hair are light black and the part above the roots is basically white; 1 point: The roots and the parts above of the newly grown hair do not show black. The above scores can be given non - integer scores according to the actual situation, and the statistical results are shown in Table 2.

[0089] Table 2 Scoring Results of the Black - Hair Effect

[0090]

[0091]

[0092] As can be seen from Table 2, the black - hair essence compositions provided in Examples 1 - 11 of the present invention have good black - hair effects. Compared with Example 2, in Examples 3 - 6, one of Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria was added respectively, and the black - hair effect scores were equivalent or slightly increased; compared with Example 2, in Examples 7 - 9, any two of Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria were added respectively, and the black - hair effect scores increased slightly; compared with Example 2, in Examples 10 - 11, four raw materials of Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria were added, and the black - hair effect scores increased significantly. It shows that when Inula helenium, Quisqualis indica, Picrorhiza scrophulariiflora, and Cotinus coggygria are added simultaneously, a synergistic effect can occur, significantly improving the black - hair effect.

[0093] Compared with Example 2, the black - hair effect scores of Comparative Examples 1 - 6 are significantly lower, indicating that the composition prepared from the raw material formula of the present invention and the composition prepared by the preparation method provided by the present invention have better black - hair effects, and changing the raw material components in the formula will reduce the black - hair effect.

[0094] The black - hair essence composition of the present invention can be added to a cosmetic matrix to prepare a black - hair product, which has the effects of promoting hair growth and nourishing hair, can fundamentally improve hair quality problems, and make the hair healthier and more beautiful.

[0095] Although embodiments of the present description have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present description, and the scope of the present description is defined by the appended claims and their equivalents.

Claims

1. A black hair essence composition, characterized in that: The composition comprises the following raw materials, in parts by weight, and is prepared according to the raw material ratio: 15-20 parts of black mulberry, 10-15 parts of polygonum multiflorum, 10-15 parts of glossy privet, 8-10 parts of salvia miltiorrhiza, 5-8 parts of angelica, 5-8 parts of chuanxiong, 8-10 parts of orientalis leaves, 5-8 parts of sesame, 5-8 parts of wolfberry, 5-8 parts of dried ginger, 5-8 parts of malt, 5-8 parts of elecampane, 5-8 parts of quisqualis, 5-8 parts of picrorhizon and 5-8 parts of cotinus coggygria; the preparation method of the hair blackening essence composition comprises the following steps: S1, weighing the medicinal raw materials, crushing and mixing them, and adding 5-10 times the weight of the mixed medicinal powder with purified water , adjust the pH to 5-7, sterilize at high temperature to obtain a sterile culture solution; S2, when the culture solution is cooled to 30°C, inoculate 0.5-1% of the weight of the medicinal materials with lactobacillus and 0.5-1% of the yeast in a sterile environment, and ferment anaerobically at 30°C for 24-48h. After the fermentation is completed, inactivate it at 121°C, and cool it to room temperature after inactivation; S3, the inactivated fermentation liquid is coarsely filtered with gauze to obtain a crude extract, the crude extract is centrifuged, and finely filtered with a ceramic membrane to obtain a refined fermentation liquid, a preservative is added to the refined fermentation liquid, sterilized at 121°C, and cooled to room temperature.

2. The black hair essence composition according to claim 1, characterized in that The weight ratio of the medicinal material powder to water is 1:

10.

3. The black hair essence composition according to claim 1, characterized in that The lactobacillus is selected from one or more of thermophilic streptococcus, bulgaricus lactobacillus, bifidobacterium longum, bifidobacterium animalis lactis, bifidobacterium breve, bifidobacterium adolescentis, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus plantarum and lactobacillus casei.

4. The black hair essence composition according to claim 1, characterized in that The preservatives are potassium sorbate and sodium benzoate, which account for 0.5% and 0.1% of the weight of the fermentation liquid respectively.

5. Use of the black hair essence composition according to any one of claims 1 to 4 in the preparation of black hair products.

6. The use according to claim 5, characterized in that: The black hair products include black hair spray, hair nutrition water, black hair essence, shampoo, hair conditioner and hair dye.

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