Humic acid organic fertilizer containing multiple active groups and preparation method thereof

Abstract

The present invention relates to the technical field of organic fertilizers, specifically, to a humic acid organic fertilizer containing multiple active groups and a preparation method thereof. It includes the following components: 40-60 parts by weight of peat, 20-35 parts by weight of organic fertilizer, 1-3 parts by weight of a modified bio-enzyme preparation, 0.5-1 parts by weight of an RW enzyme agent, 10-15 parts by weight of potassium fulvate, 15-20 parts by weight of monoammonium phosphate, and 10-15 parts by weight of potassium chloride; wherein the modified bio-enzyme preparation is prepared by surface-modifying chitinase with nano-silicon dioxide, the mass ratio of nano-silicon dioxide to chitinase is 1:10-15, and the nano-silicon dioxide particle size is 40-60nm. The modified enzyme preparation can be more evenly dispersed in an aqueous solution, while enhancing its adsorption capacity to soil particles or plant roots, especially during irrigation or rainwater erosion, helping to maintain the effective enzyme concentration in the soil and improve fertilizer utilization efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of organic fertilizers, in particular to a humic acid organic fertilizer containing multiple active groups and a preparation method thereof. Background Art

[0002] Humic acid organic fertilizer is a type of fertilizer rich in humic acid and other organic matter. It's typically made from natural resources like peat, lignite, and weathered coal. It undergoes a specific processing process and often incorporates appropriate amounts of nutrients essential for plant growth (such as nitrogen, phosphorus, and potassium) and other beneficial ingredients (such as bio-enzymes and potassium fulvate). Humic acid is a macromolecular organic compound with a complex structure and multiple active groups. It can improve soil structure, increase soil water and fertilizer retention, and promote nutrient conversion and plant absorption.

[0003] Humic acid organic fertilizer can improve the resistance of vegetables and fruits to drought, salinity, pests and diseases and other adversities during the cultivation process of vegetables and fruits. However, during irrigation or rainy seasons, the nutrients in humic acid organic fertilizer are more likely to be lost with water, resulting in resource waste and possible environmental pollution problems. In view of this, we propose a humic acid organic fertilizer containing multiple active groups and a preparation method thereof. Summary of the Invention

[0004] The object of the present invention is to provide a humic acid organic fertilizer containing multiple active groups and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides a humic acid organic fertilizer containing multiple active groups, comprising the following components: 40-60 parts by weight of peat, 20-35 parts by weight of organic fertilizer, 1-3 parts by weight of modified bioenzyme preparation, 0.5-1 part by weight of RW enzyme agent, 10-15 parts by weight of potassium fulvic acid, 15-20 parts by weight of monoammonium phosphate and 10-15 parts by weight of potassium chloride;

[0006] The modified bio-enzyme preparation is prepared by surface-modifying chitinase with nano-silica, the mass ratio of nano-silica to chitinase is 1:10-15, and the particle size of nano-silica is 40-60nm.

[0007] Organic fertilizers are treated to ensure they are safe before use. Composting and fermentation decompose the organic matter and kill pathogens, improving both efficiency and safety. Fully decomposed cow and chicken manure is an excellent organic fertilizer, improving soil structure and supporting crop growth.

[0008] Preferably, the specific preparation method of the modified bioenzyme preparation is as follows:

[0009] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:10-15;

[0010] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 3.6-8.7 g / L, maintaining an appropriate pH and ionic strength to maintain enzyme activity;

[0011] S1.3. Prepare a nanosilica suspension at a concentration of 8 mg / ml. The concentration of the nanosilica used for enzyme modification will be kept low to ensure good dispersibility. Add a chitinase solution at a concentration of 3.6-8.7 g / L dropwise to the nanosilica suspension and stir to mix thoroughly. Allow the chitinase molecules to adhere to the surface of the nanosilica particles through electrostatic adsorption, hydrophobic interaction, or hydrogen bonding.

[0012] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking and curing reaction to ensure that the enzyme molecules are firmly fixed on the nano-silica surface;

[0013] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0014] Preferably, the pH value of the chitinase solution is 5.2-5.6, and the ionic strength is 0.26-0.32M.

[0015] Preferably, in S1.3, the specific stirring conditions are: stirring at a speed of 120-180 rpm for 15-20 min using a magnetic stirrer.

[0016] Preferably, in S1.5, the mass ratio of the glutaraldehyde cross-linking agent to the chitinase solution is 1:35.

[0017] Preferably, the organic fertilizer is prepared by mixing cow dung and chicken dung in a mass ratio of 2:1.

[0018] On the other hand, the present invention provides a method for preparing a humic acid organic fertilizer containing multiple active groups, which is used for any of the above-mentioned humic acid organic fertilizers containing multiple active groups, comprising the following steps:

[0019] S2.1. Weigh the following raw materials separately: 40-60 parts by weight of peat, 20-35 parts by weight of organic fertilizer, 1-3 parts by weight of modified bio-enzyme preparation, 0.5-1 part by weight of RW enzyme agent, 10-15 parts by weight of potassium fulvate, 15-20 parts by weight of monoammonium phosphate, and 10-15 parts by weight of potassium chloride;

[0020] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0021] S2.3. Peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride are respectively put into a mixer and mixed thoroughly to obtain a mixture a;

[0022] S2.4. Adjust the moisture content of mixture a to 40%-60% to promote microbial activity. Simultaneously, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly to obtain mixture b, which is used to promote the decomposition and conversion of organic matter and enhance the biological activity of the fertilizer.

[0023] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0024] The fermentation temperature is controlled at 25-55°C, and the compost is turned regularly to increase oxygen supply. The fermentation cycle is 21-30 days to promote microbial growth. The specific fermentation cycle depends on the temperature and the specific conditions of the raw materials.

[0025] Specifically, in the early stages of fermentation, microbial activity is most vigorous and oxygen consumption is high. Therefore, in the first 3-5 days after the start of fermentation, the pile should be turned 1-2 times a day to promote uniform distribution of oxygen and heat dissipation.

[0026] As the fermentation progresses, the material temperature rises and stabilizes, and the microbial activity rate decreases. At this time, the frequency of turning the pile is adjusted to once every 1-2 days;

[0027] In the late stage of fermentation, the material temperature begins to drop and the microbial activity weakens. At this time, the frequency of turning the compost is adjusted to once every 3-4 days.

[0028] S2.7. The fermented material needs to be dried using a dryer to reduce the moisture content to 8-14%.

[0029] Preferably, in S2.3, the mixer is mixed at a speed of 80-120 rpm for 20-30 min.

[0030] Preferably, in S2.4, the mixer is mixed at a speed of 160-180 rpm for 10-15 min.

[0031] Preferably, in S2.7, the drying temperature of the dryer is 45-60°C.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] 1. In the humic acid organic fertilizer containing multiple active groups and its preparation method, the modified enzyme preparation can be more evenly dispersed in the aqueous solution, while enhancing its adsorption capacity on soil particles or plant roots, helping the enzyme to better contact the substrate and improve catalytic efficiency;

[0034] Moreover, modification reduces the loss of enzymes during application, especially during irrigation or rainwater erosion, which helps maintain the effective enzyme concentration in the soil and improves fertilizer utilization efficiency. More modified enzyme preparations mean more efficient decomposition of organic matter, making key nutrients such as nitrogen, phosphorus, and potassium in the soil easier for plants to absorb and utilize, thereby promoting plant growth.

[0035] 2. In the humic acid organic fertilizer containing multiple active groups and its preparation method, chitinase can catalyze the hydrolysis of chitin (a natural polysaccharide widely found in fungi, insect exoskeletons and certain plant residues) into N-acetylglucosamine, which helps to accelerate the decomposition of organic waste and release nutrients for plant absorption; by decomposing chitin-like substances in the soil, chitinase helps to increase the aeration and water retention of the soil, promotes the activity of beneficial microorganisms, and thus indirectly improves the growth environment of crop roots; the activity of chitinase stimulates the growth of the root system, enhances the root system's ability to absorb water and nutrients in the soil, and thus supports the rapid growth of the aboveground part, thereby increasing the overall plant height and biomass. DETAILED DESCRIPTION

[0036] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] The present invention relates to a humic acid organic fertilizer containing multiple active groups and a preparation method thereof, wherein the humic acid organic fertilizer containing multiple active groups comprises the following components: 40-60 parts by weight of peat, 20-35 parts by weight of organic fertilizer, 1-3 parts by weight of modified bioenzyme preparation, 0.5-1 part by weight of RW enzyme agent, 10-15 parts by weight of potassium fulvate, 15-20 parts by weight of monoammonium phosphate and 10-15 parts by weight of potassium chloride;

[0038] Specifically, the modified bioenzyme preparation is prepared by surface-modifying chitinase with nano-silica, the mass ratio of nano-silica to chitinase is 1:10-15, and the particle size of the nano-silica is 40-60 nm.

[0039] Specifically, the organic fertilizer is made by mixing cow dung and chicken dung in a mass ratio of 2:1.

[0040] Among them, organic fertilizers are rendered harmless before application. Through composting and fermentation, the organic matter in them is decomposed and the pathogens are killed. This can not only improve the fertilizer efficiency but also ensure the safety of use. Cow dung and chicken manure that have been fully decomposed are very good organic fertilizers, which are beneficial to the improvement of soil structure and crop growth.

[0041] Example 1

[0042] The preparation method of humic acid organic fertilizer containing multiple active groups comprises the following steps:

[0043] S2.1. Weigh the following raw materials separately: 50 parts by weight of peat, 30 parts by weight of organic fertilizer, 1 part by weight of modified bio-enzyme preparation, 0.8 parts by weight of RW enzyme agent, 13 parts by weight of potassium fulvate, 18 parts by weight of monoammonium phosphate, and 12 parts by weight of potassium chloride;

[0044] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0045] S2.3, respectively, put peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride into a mixer, and mix at a speed of 80-120 rpm for 20-30 min to fully mix and obtain a mixture a;

[0046] S2.4. Adjust the water content of mixture a to 55%. At the same time, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly. Mix at a speed of 160-180 rpm for 10-15 minutes to obtain mixture b.

[0047] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0048] The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days.

[0049] S2.7. The fermented material needs to be dried. Use a dryer at a drying temperature of 45-60°C to reduce the moisture content of the fermented material to 12%.

[0050] The specific preparation method of the modified bioenzyme preparation is as follows:

[0051] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:10;

[0052] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 3.6 g / L, wherein the chitinase solution has a pH of 5.3 and an ionic strength of 0.28 M;

[0053] S1.3. Prepare a nano-silica suspension at a concentration of 8 mg / ml, and add a chitinase solution at a concentration of 3.6 g / L dropwise to the nano-silica suspension. Stir with a magnetic stirrer at 120-180 rpm for 15-20 minutes to mix thoroughly.

[0054] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking curing reaction, wherein the mass ratio of glutaraldehyde crosslinking agent to chitinase solution is 1:35;

[0055] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0056] Example 2

[0057] The preparation method of humic acid organic fertilizer containing multiple active groups comprises the following steps:

[0058] S2.1. Weigh the following raw materials separately: 50 parts by weight of peat, 30 parts by weight of organic fertilizer, 2 parts by weight of modified bio-enzyme preparation, 0.8 parts by weight of RW enzyme agent, 13 parts by weight of potassium fulvate, 18 parts by weight of monoammonium phosphate, and 12 parts by weight of potassium chloride;

[0059] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0060] S2.3, respectively, put peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride into a mixer, and mix at a speed of 80-120 rpm for 20-30 min to fully mix and obtain a mixture a;

[0061] S2.4. Adjust the water content of mixture a to 60%. At the same time, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly. Mix at a speed of 160-180 rpm for 10-15 minutes to obtain mixture b.

[0062] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0063] The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days.

[0064] S2.7. The fermented material needs to be dried. Use a dryer at a drying temperature of 45-60°C to reduce the moisture content of the fermented material to 12%.

[0065] The specific preparation method of the modified bioenzyme preparation is as follows:

[0066] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:10;

[0067] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 3.6 g / L, wherein the chitinase solution has a pH of 5.3 and an ionic strength of 0.28 M;

[0068] S1.3. Prepare a nano-silica suspension at a concentration of 8 mg / ml, and add a chitinase solution at a concentration of 3.6 g / L dropwise to the nano-silica suspension. Stir with a magnetic stirrer at 120-180 rpm for 15-20 minutes to mix thoroughly.

[0069] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking curing reaction, wherein the mass ratio of glutaraldehyde crosslinking agent to chitinase solution is 1:35;

[0070] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0071] Example 3

[0072] The preparation method of humic acid organic fertilizer containing multiple active groups comprises the following steps:

[0073] S2.1. Weigh the following raw materials separately: 50 parts by weight of peat, 30 parts by weight of organic fertilizer, 3 parts by weight of modified bio-enzyme preparation, 0.8 parts by weight of RW enzyme agent, 13 parts by weight of potassium fulvate, 18 parts by weight of monoammonium phosphate, and 12 parts by weight of potassium chloride;

[0074] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0075] S2.3, respectively, put peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride into a mixer, and mix at a speed of 80-120 rpm for 20-30 min to fully mix and obtain a mixture a;

[0076] S2.4. Adjust the water content of mixture a to 55%. At the same time, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly. Mix at a speed of 160-180 rpm for 10-15 minutes to obtain mixture b.

[0077] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0078] The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days.

[0079] S2.7. The fermented material needs to be dried. Use a dryer at a drying temperature of 45-60°C to reduce the moisture content of the fermented material to 12%.

[0080] The specific preparation method of the modified bioenzyme preparation is as follows:

[0081] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:10;

[0082] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 3.6 g / L, wherein the chitinase solution has a pH of 5.3 and an ionic strength of 0.28 M;

[0083] S1.3. Prepare a nano-silica suspension at a concentration of 8 mg / ml, and add a chitinase solution at a concentration of 3.6 g / L dropwise to the nano-silica suspension. Stir with a magnetic stirrer at 120-180 rpm for 15-20 minutes to mix thoroughly.

[0084] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking curing reaction, wherein the mass ratio of glutaraldehyde crosslinking agent to chitinase solution is 1:35;

[0085] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0086] Example 4

[0087] The preparation method of humic acid organic fertilizer containing multiple active groups comprises the following steps:

[0088] S2.1. Weigh the following raw materials separately: 50 parts by weight of peat, 30 parts by weight of organic fertilizer, 3 parts by weight of modified bio-enzyme preparation, 0.8 parts by weight of RW enzyme agent, 13 parts by weight of potassium fulvate, 18 parts by weight of monoammonium phosphate, and 12 parts by weight of potassium chloride;

[0089] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0090] S2.3, respectively, put peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride into a mixer, and mix at a speed of 80-120 rpm for 20-30 min to fully mix and obtain a mixture a;

[0091] S2.4. Adjust the water content of mixture a to 45%. At the same time, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly. Mix at a speed of 160-180 rpm for 10-15 minutes to obtain mixture b.

[0092] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0093] The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days.

[0094] S2.7. The fermented material needs to be dried. Use a dryer at a drying temperature of 45-60°C to reduce the moisture content of the fermented material to 12%.

[0095] The specific preparation method of the modified bioenzyme preparation is as follows:

[0096] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:13;

[0097] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 6.3 g / L, wherein the chitinase solution has a pH of 5.2 and an ionic strength of 0.26 M;

[0098] S1.3. Prepare a nano-silica suspension at a concentration of 8 mg / ml, and add a chitinase solution at a concentration of 6.3 g / L dropwise to the nano-silica suspension. Stir with a magnetic stirrer at 120-180 rpm for 15-20 minutes to mix thoroughly.

[0099] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking curing reaction, wherein the mass ratio of glutaraldehyde crosslinking agent to chitinase solution is 1:35;

[0100] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0101] Example 5

[0102] The preparation method of humic acid organic fertilizer containing multiple active groups comprises the following steps:

[0103] S2.1. Weigh the following raw materials separately: 50 parts by weight of peat, 30 parts by weight of organic fertilizer, 3 parts by weight of modified bio-enzyme preparation, 0.8 parts by weight of RW enzyme agent, 13 parts by weight of potassium fulvate, 18 parts by weight of monoammonium phosphate, and 12 parts by weight of potassium chloride;

[0104] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0105] S2.3, respectively, put peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride into a mixer, and mix at a speed of 80-120 rpm for 20-30 min to fully mix and obtain a mixture a;

[0106] S2.4. Adjust the water content of mixture a to 55%. At the same time, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly. Mix at a speed of 160-180 rpm for 10-15 minutes to obtain mixture b.

[0107] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0108] The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days.

[0109] S2.7. The fermented material needs to be dried. Use a dryer at a drying temperature of 45-60°C to reduce the moisture content of the fermented material to 12%.

[0110] The specific preparation method of the modified bioenzyme preparation is as follows:

[0111] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:15;

[0112] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 8.7 g / L, wherein the chitinase solution has a pH of 5.2 and an ionic strength of 0.30 M;

[0113] S1.3. Prepare a nano-silica suspension at a concentration of 8 mg / ml, and add a chitinase solution at a concentration of 8.7 g / L dropwise to the nano-silica suspension. Stir with a magnetic stirrer at 120-180 rpm for 15-20 minutes to mix thoroughly.

[0114] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking curing reaction, wherein the mass ratio of glutaraldehyde crosslinking agent to chitinase solution is 1:35;

[0115] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0116] Example 6

[0117] The preparation method of humic acid organic fertilizer containing multiple active groups comprises the following steps:

[0118] S2.1. Weigh the following raw materials separately: 60 parts by weight of peat, 35 parts by weight of organic fertilizer, 3 parts by weight of modified bio-enzyme preparation, 1 part by weight of RW enzyme agent, 15 parts by weight of potassium fulvate, 20 parts by weight of monoammonium phosphate, and 15 parts by weight of potassium chloride;

[0119] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0120] S2.3, respectively, put peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride into a mixer, and mix at a speed of 80-120 rpm for 20-30 min to fully mix and obtain a mixture a;

[0121] S2.4. Adjust the water content of mixture a to 55%. At the same time, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly. Mix at a speed of 160-180 rpm for 10-15 minutes to obtain mixture b.

[0122] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0123] The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days.

[0124] S2.7. The fermented material needs to be dried. Use a dryer at a drying temperature of 45-60°C to reduce the moisture content of the fermented material to 12%.

[0125] The specific preparation method of the modified bioenzyme preparation is as follows:

[0126] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:13;

[0127] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 6.3 g / L, wherein the chitinase solution has a pH of 5.2 and an ionic strength of 0.26 M;

[0128] S1.3. Prepare a nano-silica suspension at a concentration of 8 mg / ml, and add a chitinase solution at a concentration of 6.3 g / L dropwise to the nano-silica suspension. Stir with a magnetic stirrer at 120-180 rpm for 15-20 minutes to mix thoroughly.

[0129] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking curing reaction, wherein the mass ratio of glutaraldehyde crosslinking agent to chitinase solution is 1:35;

[0130] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0131] Example 7

[0132] The preparation method of humic acid organic fertilizer containing multiple active groups comprises the following steps:

[0133] S2.1. Weigh the following raw materials separately: 40 parts by weight of peat, 20 parts by weight of organic fertilizer, 3 parts by weight of modified bio-enzyme preparation, 0.5 parts by weight of RW enzyme agent, 10 parts by weight of potassium fulvate, 15 parts by weight of monoammonium phosphate, and 10 parts by weight of potassium chloride;

[0134] S2.2, peat and organic fertilizer are dried, crushed, and sieved to control the particle size to 3-5mm;

[0135] S2.3, respectively, put peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride into a mixer, and mix at a speed of 80-120 rpm for 20-30 min to fully mix and obtain a mixture a;

[0136] S2.4. Adjust the water content of mixture a to 55%. At the same time, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly. Mix at a speed of 160-180 rpm for 10-15 minutes to obtain mixture b.

[0137] S2.5. Transfer mixture b into a fermentation tank for primary fermentation;

[0138] The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days.

[0139] S2.7. The fermented material needs to be dried. Use a dryer at a drying temperature of 45-60°C to reduce the moisture content of the fermented material to 12%.

[0140] The specific preparation method of the modified bioenzyme preparation is as follows:

[0141] S1.1. Weigh nanosilica and chitinase in a mass ratio of 1:13;

[0142] S1.2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 6.3 g / L, wherein the chitinase solution has a pH of 5.2 and an ionic strength of 0.26 M;

[0143] S1.3. Prepare a nano-silica suspension at a concentration of 8 mg / ml, and add a chitinase solution at a concentration of 6.3 g / L dropwise to the nano-silica suspension. Stir with a magnetic stirrer at 120-180 rpm for 15-20 minutes to mix thoroughly.

[0144] S1.5, adding glutaraldehyde crosslinking agent to carry out crosslinking curing reaction, wherein the mass ratio of glutaraldehyde crosslinking agent to chitinase solution is 1:35;

[0145] S1.6. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

[0146] Comparative Example 1

[0147] The method of Example 4 was adopted, but no modified bioenzyme preparation was added to the humic acid organic fertilizer raw material containing multiple active groups.

[0148] Comparative Example 2

[0149] The method of Example 4 was used without using nano-silica to modify the chitinase.

[0150] The present invention prepares humic acid organic fertilizer containing multiple active groups by modifying the bioenzyme preparation, wherein the performance index test items and test standards of the humic acid organic fertilizer are as follows:

[0151] Tomatoes were selected as the cultivation object and the experiment was carried out through the following steps:

[0152] The humic acid organic fertilizer containing multiple active groups prepared by the methods of Examples 1-7 and Comparative Examples 1-2 was selected, and representative plots were selected to ensure that the soil type, fertility, irrigation conditions, etc. were basically consistent. In addition to fertilization treatment, other management measures (such as sowing density, irrigation, pest and disease control, etc.) needed to be consistent to eliminate interference from other variables;

[0153] Regularly record tomato plant height, root length, and other growth indicators. Root length is monitored primarily through the taproot. Soil samples were taken before and after the experiment to determine fertilizer residues in the soil to assess fertilizer utilization and potential environmental impacts.

[0154] At the same time, considering the growth cycle of tomatoes, the experiment needs to cover at least one complete growing season.

[0155] The humic acid organic fertilizers prepared in Examples 1-7 and Comparative Examples 1-2 were tested using the above standards, and the obtained data are shown in Table 1:

[0156] Table 1 Fertilizer efficiency performance data of humic acid organic fertilizers of Examples 1-7 and Comparative Examples 1-2

[0157] Example Plant height m Root length m Fertilizer utilization rate (%) Example 1 1.28 1.38 36.8 Example 2 1.45 1.46 43.6 Example 3 1.62 1.54 51.6 Example 4 1.86 1.63 57.8 Example 5 1.97 1.70 59.1 Example 6 1.89 1.65 58.1 Example 7 1.82 1.59 56.4 Comparative Example 1 0.86 1.06 26.8 Comparative Example 2 1.14 1.17 28.9

[0158] The above data fully show that compared with Comparative Examples 1-2, the humic acid organic fertilizer containing multiple active groups prepared by the modified bio-enzyme preparation of Examples 1-7 has the following fertilizer effects:

[0159] It can be seen from Examples 1-3 that:

[0160] As the content of modified enzyme preparations continued to increase, the plant height and root length of tomatoes increased significantly, and the fertilizer utilization rate was also significantly improved;

[0161] Among them, the modified enzyme preparation can be more evenly dispersed in the aqueous solution, while enhancing its adsorption capacity to soil particles or plant roots, helping the enzyme to better contact the substrate and improve catalytic efficiency;

[0162] Moreover, modification reduces the loss of enzymes during application, especially during irrigation or rainwater erosion, which helps maintain the effective enzyme concentration in the soil and improves fertilizer utilization efficiency. More modified enzyme preparations mean more efficient decomposition of organic matter, making key nutrients such as nitrogen, phosphorus, and potassium in the soil easier for plants to absorb and utilize, thereby promoting plant growth.

[0163] Furthermore, by comparing Examples 3-5, it can be seen that:

[0164] As the content of chitinase in the modified bio-enzyme preparation continued to increase, the plant height and root length of tomatoes increased significantly, and the fertilizer utilization rate was also significantly improved;

[0165] Chitinase catalyzes the hydrolysis of chitin (a natural polysaccharide widely found in fungi, insect exoskeletons, and some plant residues) into N-acetylglucosamine, which helps accelerate the decomposition of organic waste and releases nutrients for plant absorption. By breaking down chitin-like substances in the soil, chitinase helps increase soil aeration and water retention, promotes the activity of beneficial microorganisms, and thus indirectly improves the growth environment of crop roots. Chitinase activity stimulates root growth, enhancing the root system's ability to absorb water and nutrients from the soil, thereby supporting the rapid growth of the aboveground part and increasing overall plant height and biomass.

[0166] Furthermore, by comparing Example 4, Example 6 and Example 7, it can be seen that:

[0167] When the content of modified enzyme preparation and chitinase remained unchanged and other raw materials were changed, the plant height, root length and fertilizer utilization efficiency of tomatoes changed little;

[0168] According to the above test experiments, the humic acid organic fertilizer prepared according to Example 4 has the best fertilizer efficiency performance, so Example 4 is taken as the best example;

[0169] By comparing Example 4 with Comparative Examples 1-2, it can be seen that:

[0170] In Comparative Example 1, when the method of Example 4 was used and no modified bio-enzyme preparation was added to the humic acid organic fertilizer raw material containing multiple active groups, the plant height, root length and fertilizer utilization rate of tomatoes were significantly reduced. This shows that the modified bio-enzyme preparation plays a dominant role in the plant height, root length and fertilizer utilization rate of tomatoes.

[0171] In Comparative Example 2, when chitinase was not modified with nanosilica, the plant height, root length, and fertilizer utilization efficiency of tomatoes were significantly improved compared to Comparative Example 1. This demonstrates that chitinase itself has a positive effect on promoting tomato growth and improving fertilizer utilization. It can help decompose organic matter such as chitin in the soil, promote the release and absorption of nutrients, and improve the soil environment. However, the modification process further enhances the stability and efficiency of chitinase, resulting in the modified bioenzyme preparation showing superior performance in improving crop growth performance and fertilizer utilization efficiency.

[0172] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely preferred examples of the present invention and are not intended to limit the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. Humic acid organic fertilizer containing multiple active groups, characterized in that: The invention comprises the following components: 40-60 parts by weight of peat, 20-35 parts by weight of organic fertilizer, 1-3 parts by weight of modified biological enzyme preparation, 0.5-1 parts by weight of RW enzyme agent, 10-15 parts by weight of potassium fulvic acid, 15-20 parts by weight of monoammonium phosphate and 10-15 parts by weight of potassium chloride; The modified bioenzyme preparation is prepared by surface-modifying chitinase with nano-silicon dioxide, the mass ratio of nano-silicon dioxide to chitinase is 1:10-15, and the particle size of nano-silicon dioxide is 40-60 nm. The specific preparation method of the modified bioenzyme preparation is as follows: S1.

1. Weigh nanosilica and chitinase at a mass ratio of 1:10-15; S1.

2. Dissolve chitinase in phosphate buffer to obtain a chitinase solution with a concentration of 3.6-8.7 g / L; S1.

3. Prepare a nano-silica suspension with a concentration of 8 mg / ml, and dropwise add a chitinase solution with a concentration of 3.6-8.7 g / L to the nano-silica suspension. Stir and mix thoroughly to allow the chitinase molecules to adhere to the surface of the nano-silica particles. S1.4, adding glutaraldehyde crosslinking agent to carry out crosslinking and curing reaction to ensure that the enzyme molecules are firmly fixed on the nano-silica surface; S1.

5. After the reaction is completed, the modified bio-enzyme preparation is washed with deionized water to remove unbound enzyme molecules and small molecular impurities. The washed modified bio-enzyme preparation is vacuum dried to remove moisture to obtain a dry powdered modified bio-enzyme preparation.

2. The humic acid organic fertilizer containing multiple active groups according to claim 1, characterized in that, The pH value of the chitinase solution is 5.2-5.6, and the ionic strength is 0.26-0.32M.

3. The humic acid organic fertilizer containing multiple active groups according to claim 1, characterized in that, In S1.3, the specific stirring conditions are: stirring at a speed of 120-180 rpm for 15-20 minutes using a magnetic stirrer.

4. The humic acid organic fertilizer containing multiple active groups according to claim 1, characterized in that, In the above-mentioned S1.4, the mass ratio of the glutaraldehyde cross-linking agent to the chitinase solution is 1:

35.

5. The humic acid organic fertilizer containing multiple active groups according to claim 1, characterized in that, The organic fertilizer is prepared by mixing cow dung and chicken dung in a mass ratio of 2:

1.

6. A method for preparing a humic acid organic fertilizer containing multiple active groups, for preparing the humic acid organic fertilizer containing multiple active groups as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: S2.

1. Weigh the following raw materials separately: 40-60 parts by weight of peat, 20-35 parts by weight of organic fertilizer, 1-3 parts by weight of modified bio-enzyme preparation, 0.5-1 part by weight of RW enzyme agent, 10-15 parts by weight of potassium fulvate, 15-20 parts by weight of monoammonium phosphate, and 10-15 parts by weight of potassium chloride; S2.

2. Peat and organic fertilizer are dried and crushed, and the particle size is controlled to 3-5mm by screening; S2.

3. Peat, organic fertilizer, potassium fulvic acid, monoammonium phosphate and potassium chloride are respectively put into a mixer and mixed thoroughly to obtain a mixture a; S2.

4. Adjust the moisture content of mixture a to 40%-60% to promote microbial activity. Simultaneously, add the modified bio-enzyme preparation and RW enzyme agent to the mixer and continue stirring to mix them evenly to obtain mixture b, which is used to promote the decomposition and conversion of organic matter. S2.

5. Transfer mixture b into a fermentation tank for primary fermentation; The fermentation temperature is controlled at 25-55°C, the compost is turned regularly to increase oxygen supply, and the fermentation period is 21-30 days. S2.

6. The fermented materials need to be dried and the moisture content should be reduced to 8-14% using a dryer.

7. The method for preparing humic acid organic fertilizer containing multiple active groups according to claim 6, wherein: In the above S2.3, the mixer is mixed at a speed of 80-120 rpm for 20-30 min.

8. The method for preparing humic acid organic fertilizer containing multiple active groups according to claim 6, wherein: In the above S2.4, the mixer is mixed at a speed of 160-180 rpm for 10-15 minutes.

9. The method for preparing humic acid organic fertilizer containing multiple active groups according to claim 6, wherein: In said S2.6, the drying temperature of the dryer is 45-60°C.