A mite-killing composition and its application

Abstract

The present invention belongs to the field of pesticide technology and relates to an acaricidal composition and its use. The acaricidal composition comprises active ingredient A and active ingredient B; active ingredient A is a compound of formula (I), and active ingredient B is emamectin benzoate. The acaricidal composition exhibits a synergistic effect against harmful mites within a certain ratio range, particularly effective against root mites, and can reduce the dosage and cost of the pesticide.

Description

Technical Field

[0001] The invention belongs to the field of pesticides, and particularly relates to an acaricidal composition and application thereof. Background Art

[0002] In recent years, frequent transportation of garlic seeds, high temperatures in autumn, winter, and spring, continuous garlic cropping year-round, and farmers' lack of attention to pest control have led to a significant outbreak of the spiny-legged root mite (Rhizoglyphus echinopus (Fumouze et Robin)) in garlic fields. These tiny, highly reproductive mites can carry microbial pathogens and exhibit strong resistance to adversity. They coexist with root maggots and leek maggots, making them a prominent pest of garlic, severely impacting both yield and quality.

[0003] Emamectin benzoate (emamectin benzoate) is a semisynthetic antibiotic insecticide with both stomach and contact effects. Emamectin benzoate primarily enters the insect's stomach during feeding, exerting its toxic effect. It has a limited contact effect on some insects with thinner body walls, but is inactive against their eggs. By controlling the opening of chloride ion channels, it disrupts information transmission within the insect's nervous system, inhibiting normal physiological activity and disrupting the activity of related detoxification enzymes, ultimately leading to paralysis and death.

[0004] The spiny-footed root mite, with its adult and nymphal mites, attacks the leaf sheaths, pseudostems, and bulbs at the base of the fibrous roots. The affected plants have fewer fibrous roots, underdeveloped root systems, and in severe cases, the fibrous roots may fall off. When feeding on pseudostems or bulbs, they generally feed from the outside inwards, causing necrosis of the affected tissue cells, which then turn brown, rot, and stink, similar to the symptoms of soft rot. In the later stages of the damage, it can indirectly cause bacterial soft rot and red root rot. The aboveground parts initially show slow growth, with short, thin plants, yellowing and drying of the lower leaves, followed by the upper leaves gradually turning yellow and being very easy to pull out by hand. In severe cases, the entire plant withers and dies. Reasonable compounding or mixing of chemical agents has the positive characteristics of expanding the insecticide and acaricide spectrum, improving the control effect, extending the appropriate period for application, reducing the amount of medicine used, reducing medicine damage, reducing residues, and delaying the occurrence of pest tolerance and resistance. The applicant surprisingly found through indoor toxicity experiments and field efficacy tests that the combination of the compound of formula (I) and emamectin benzoate has a significant synergistic effect on the spiny-legged root mite, can reduce the amount of medicine used, reduce the cost of use, and promote crop yield. Summary of the Invention

[0005] In order to solve the above-mentioned problems existing in the prior art, the present invention provides a mite-killing composition, which can effectively prevent harmful mites, especially spiny-legged root mites, and exhibits a synergistic effect within a certain proportion range, thereby reducing the cost of use, reducing the dosage, extending the effective period and delaying the development of drug resistance.

[0006] In order to solve the above technical problems, the present invention provides the following technical solution: an acaricidal composition, comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound of formula (I):

[0007] Active ingredient B is emamectin benzoate;

[0008] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 50:1;

[0009] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 30:1;

[0010] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to emamectin benzoate is 1:20 to 20:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to emamectin benzoate is 1:20, 1:15, 1:10, 1:5, 6:1, 12:1, 16:1, 20:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to emamectin benzoate is 1:15 to 20:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to emamectin benzoate is 1:15, 1:10, 1:5, 6:1, 12:1, 16:1, 20:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to emamectin benzoate is 1:10 to 20:1;

[0016] Furthermore, based on the total weight of the acaricidal composition being 100 wt%, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 0.1 to 95 wt%;

[0017] Furthermore, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1 to 80 wt%;

[0018] Furthermore, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 2 to 60 wt%;

[0019] Furthermore, the acaricidal composition further comprises, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0020] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0021] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0022] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0023] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0024] Disintegrants: The disintegrants are selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0025] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0026] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0027] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0028] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0029] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0030] Synergists are selected from synergist, piperonyl butoxide; and / or

[0031] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0032] Furthermore, the acaricidal composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is selected from a solid formulation and / or a liquid formulation;

[0033] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0034] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0035] Furthermore, the solid preparation is selected from wettable powder, water dispersible granules, soluble granules; the liquid preparation is selected from emulsifiable concentrate, water emulsion, suspension concentrate, suspoemulsion;

[0036] Furthermore, the harmful mite is of the genus Rhizoctonia of the family Acaridae in the order Acarina;

[0037] Further, the root mite genus is the spiny-foot root mite;

[0038] Furthermore, the acaricidal composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled or the medium where they grow.

[0039] Beneficial effects of the present invention:

[0040] (1) The acaricidal composition of the present invention has a significant synergistic effect at a certain ratio, especially having a good control effect on spiny-legged root mites;

[0041] (2) It is safe and highly effective, can delay the development of pesticide resistance in pest mites, and reduce the amount of pesticides used;

[0042] (3) It has a long lasting effect and can effectively control the damage of crops to mites throughout the entire growth period. It is safe for crops, non-target organisms, beneficial organisms and natural enemies, and has the effect of increasing production and ensuring income. DETAILED DESCRIPTION

[0043] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0044] Preparation example:

[0045] Preparation Example 1: 22% formula (I) compound·emamectin benzoate suspension (18:4)

[0046] Preparation formula: 18% compound of formula (I), 4% emamectin benzoate, 1.5% sodium lignin sulfonate, 3.5% fatty amine polyoxyethylene ether, 2% polycarboxylate, 5% naphthalene sulfonate, 0.45% sodium p-hydroxybenzoate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.2% organosilicon defoamer, and deionized water to make up the balance;

[0047] Preparation method: According to the formula ratio of the embodiment, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, deionized water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0048] Preparation Example 2: 4.3% compound of formula (I)·emamectin benzoate emulsifiable concentrate (4:0.3)

[0049] Preparation formula: 4% compound of formula (I), 0.3% emamectin benzoate, 1% BHT, 15% propylene carbonate, 10% DMF, 10% cyclohexanone, 3% calcium dodecylbenzenesulfonate, 15% fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0050] Preparation method: Add the active ingredients into the cosolvent according to the formula ratio of the embodiment, and add surfactants and other functional additives thereto, stir and mix them evenly in a stirring mixing kettle to obtain the emulsifiable concentrate product.

[0051] Preparation Example 3: 9% compound of formula (I)·emamectin benzoate aqueous emulsion (7.5:1.5)

[0052] Preparation formula: 7.5% compound of formula (I), 1.5% emamectin benzoate, 2% BHT, 6% xylene, 20% cyclohexanone, 1% calcium dodecylbenzenesulfonate, 8% ethylene oxide-propylene oxide copolymer, 3.5% glycerol, 5% glycerol, 0.2% silicone defoamer, 0.1% xanthan gum, 0.2% kason, and deionized water to make up the balance;

[0053] Preparation method: According to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase. Deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shear, the water phase is added to the oil phase, and finally a thickener, preservative and defoaming agent are added to form a well-dispersed water emulsion product.

[0054] Indoor toxicity

[0055] Examples refer to "Guidelines for Indoor Bioassay of Pesticides Part 7: Determination of Combined Effects of Mixtures NY / T1154.7-2006"

[0056] Test insect source: spiny-legged root mite (raised in the R&D center)

[0057] Select female adult mites raised indoors with consistent physiological status. The temperature is (25±1)°C, the relative humidity is 65%±5%, and the photoperiod is 16 / 8h (L / D).

[0058] Preparation: Dissolve the original drug in acetone and dilute with a 0.1% Tween-80 solution. Prepare separate single-dose stock solutions. Design five groups of mixes based on the mixing objectives and drug activity. Prepare five series of mass concentrations for each single dose and each mix using the same ratio.

[0059] Test method: Take a glass tube with a diameter of 1 cm and roll it evenly with 250ul of drug solution. After the drug solution is naturally dried, 15 female adult mites are sucked into the glass tube and sealed with a 200-mesh gauze.

[0060] Test repetition: Each treatment shall be repeated at least 4 times, and the number of test insects in each dose treatment shall be at least 60. A treatment without pesticide (including all organic solvents and emulsifiers) shall be set as blank control.

[0061] Data statistics and analysis: 48 h after treatment, the mortality of the test insects was checked and the total number of mites and the number of dead mites were recorded respectively (death standard: use a needle tip to poke the mite appendages, those that do not move are dead, and those that move are alive).

[0062] Based on the survey data, the adjusted mortality rate of each treatment was calculated.

[0063] Calculate using the following formula, with the results rounded to two decimal places:

[0064]

[0065] Where:

[0066] P——mortality rate, in percentage (%);

[0067] K——indicates the number of dead insects, the unit is head;

[0068] N——represents the total number of insects processed, in heads.

[0069]

[0070] Where:

[0071] P1——adjusted mortality rate, in percentage (%);

[0072] P t ——Treatment mortality rate, expressed in percentage (%);

[0073] P0 - blank control mortality rate, in percentage (%).

[0074] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0075] The data were processed using the probability value analysis method. The DPS statistical analysis system can be used to analyze and calculate the LC of the toxicity regression line. 50 The activity of the test agent on the biological test material was evaluated by using the b value and its 95% confidence limit.

[0076] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0077]

[0078] Where:

[0079] ATI - measured toxicity index of mixture;

[0080] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0081] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0082] TTI=TI A *P A +TI B *P B

[0083] Where:

[0084] TTI – Theoretical Toxicity Index of Mixtures;

[0085] TI A ——Agent toxicity index;

[0086] P A ——The percentage of agent A in the mixture, in percentage (%);

[0087] TI B ——Toxicity index of agent B;

[0088] P B ——The percentage of agent B in the mixture, in percentage (%).

[0089]

[0090] Where:

[0091] CTC – Co-toxicity coefficient;

[0092] ATI - measured toxicity index of mixture;

[0093] TTI - Theoretical Toxicity Index of Mixture.

[0094] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0095] Test results:

[0096] The test results in Table 1 show that emamectin benzoate has a high toxicity to the spiny-legged root mite, LC 50 The concentration of the compound of formula (I) and emamectin benzoate was 3.9086 mg / L, and the concentration of the compound of formula (I) and emamectin benzoate showed a synergistic effect in the range of 1:15 to 20:1.

[0097] Table 1 Combined toxicity test of different ratios of compound of formula (I) and emamectin benzoate against Rhizoctonia spinulosa

[0098]

[0099] Example 2 Field trial to control garlic root mite

[0100] Test crops: garlic, variety: Jinxiang white garlic;

[0101] Test subjects: Rhizopus radicifolius;

[0102] The experimental site is Jinxiang, Jining City, Shandong Province. The experimental field is flat, the previous crop is soybean, and the soil is sandy loam with medium fertility.

[0103] Test agent:

[0104] Table 2 Experimental treatment and dosage

[0105] Experimental treatment Experimental drugs Dosage mL / kg seeds 1 4.3% formula (Ⅰ) compound·emamectin benzoate emulsifiable concentrate (4:0.3) 0.4 2 9% compound of formula (I)·emamectin benzoate aqueous emulsion (7.5:1.5) 0.2 3 2% emamectin benzoate aqueous emulsion 0.5 4 6% Imidacloprid Suspension Seed Coating Agent 8 5 Clean Water Treatment (CK) /

[0106] Experimental design: The experiment set up 4 pesticide seed treatments and 1 water treatment (CK), with random arrangement and 3 replicates for each treatment. The plot area was 20m 2 Before seed mixing, dilute the agent with 100mL of water. On October 2, 2021, seed mixing was carried out. Garlic cloves harvested that year were selected, with a single clove weight of about 5g, firm and plump, white in color, without disease spots or wounds, and of uniform size. After mixing, they were dried and sown the next day. The planting density was 600,000 plants / hm2. 2 .

[0107] Investigation method: The damage situation of spiny-footed root mites was investigated on November 20, 2021, the peak period of infestation. 15 plants were randomly sampled from each treatment to investigate the spiny-footed root mites in the diseased plants (spiny-footed root mite investigation method: investigate the total number of mites on the pseudostem and bulb, combining above-ground observation with indoor microscopic examination after pulling out the seedlings).

[0108] During the harvest period, the incidence of diseased plants in each plot was investigated. Fifty plants were randomly surveyed in each treatment, and the number of diseased plants was recorded, and the incidence rate and control effect were calculated.

[0109] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0110]

[0111]

[0112] Measurement indicators and methods: 30 plants were sampled from each treatment on June 10, 2022. The plant height was measured with a tape measure, and the transverse diameter of the bulb was measured with a vernier caliper (based on the distance from the stem disk to the top of the longest leaf).

[0113] During the experiment, garlic in each treatment plot grew well and no phytotoxicity was observed in any treatment.

[0114] In early May 2022, garlic stalks were harvested to measure yield; in mid-June 2022, garlic bulbs were harvested to measure total yield.

[0115] The weather was good on the day and during the test.

[0116] Effects of different treatments on the control of garlic root mites

[0117] Test results:

[0118] Table 3 shows the control effects of each treatment on garlic root mite. The number of root mites, diseased plant rate and diseased fingers in each treatment were lower than those in the control, and the control efficacy ranged from 34.17% to 85.78%. Among them, the mixture of emamectin benzoate of formula (I) compound showed better control effect. The control efficacy of 4.3% formula (I) compound·emamectin benzoate emulsifiable concentrate (4:0.3) and 9% formula (I) compound·emamectin benzoate emulsifiable concentrate (7.5:1.5) were 85.78% and 84.90%, respectively.

[0119] Table 3 Control effects of different treatments on garlic root mites

[0120]

[0121]

[0122] Note: The number of Rhizoctonia suspensa (heads), diseased plant rate (%), and control efficacy (%) in the above table are the averages of each replicate. Lowercase letters indicate significant differences at the 5% level.

[0123] Effects of different treatments on garlic plant height As shown in Table 4, the plant height of garlic treated with seed dressing was greater than that of the control, among which the 4.3% formula (I) compound·emamectin benzoate emulsifiable concentrate (4:0.3) was the highest, significantly higher than the blank control.

[0124] Table 4 Effects of different treatments on garlic plant height

[0125]

[0126] Note: Lowercase letters represent significant differences at the 5% level.

[0127] Effects of different treatments on the transverse diameter of garlic bulbs

[0128] As shown in Table 5, the transverse diameters of garlic bulbs in the seed dressing treatments were larger than those in the control, but the differences were not significant.

[0129] Table 5 Effects of different treatments on the transverse diameter of garlic bulbs

[0130]

[0131] Note: Lowercase letters represent significant differences at the 5% level.

[0132] Effects of different treatments on the yield of garlic stalks and bulbs

[0133] As shown in Table 6, compared with the control, the yield of garlic stalks and garlic heads in each treatment with seed dressing agent increased.

[0134] Table 6 Effects of different treatments on garlic stalk and garlic bulb yield

[0135]

[0136]

[0137] According to the field investigation, no adverse reaction of leaves to the pesticide was found in any treatment, indicating that all treatments were safe for garlic growth at the concentrations used in the experiment.

[0138] Indoor toxicity assays and field tests on garlic have shown that the acaricidal composition of the present invention demonstrates excellent control efficacy against Rhizopus spinulosa. The acaricidal composition or formulation obtained by compounding the present invention exhibits significant control efficacy, outperforming single agents in delaying the development of resistance and prolonging drug retention. Furthermore, no phytotoxicity to crops was observed with the compounded formulation in the tests, demonstrating that the enhanced synergistic acaricidal efficacy of the acaricidal composition or formulation can reduce production and usage costs, be safe for crops, and promote increased crop yields.

[0139] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, characterized in that: The acaricidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound of formula (I): (I), active ingredient B is emamectin benzoate, and the mass ratio of the compound of formula (I) to emamectin benzoate is 1:15~20:

1.

2. The acaricidal composition according to claim 1, characterized in that Based on the total weight of the acaricidal composition being 100 wt %, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1 to 80 wt %.

3. The acaricidal composition according to claim 1, characterized in that In addition to the active ingredients, the acaricidal composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

4. The acaricidal composition according to claim 3, characterized in that The acaricidal composition can be prepared into an agriculturally acceptable formulation, which is selected from a solid formulation and / or a liquid formulation.

5. The acaricidal composition according to claim 4, characterized in that The solid preparation is selected from wettable powder, water-dispersible granules, and soluble granules; the liquid preparation is selected from emulsifiable concentrate, aqueous emulsion, suspension, and suspoemulsion.

6. Use of the acaricidal composition according to any one of claims 1 to 5 for controlling harmful mites, characterized in that: The harmful mite is a pest mite of the genus Rhizoctonia of the family Acaridae in the order Acarina; and the harmful mite of the genus Rhizoctonia is the spiny-legged Rhizoctonia.

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