Quickly reconstituted eel protein powder and preparation method thereof

Abstract

The application discloses quick-dissolved eel protein powder and a preparation method thereof, and belongs to the field of food processing. The preparation method of the quick-dissolved eel protein powder comprises the following steps: (1) pretreatment: thawing and peeling eels, stirring and crushing to obtain eel crushing products; adding a phosphate buffer solution, stirring and crushing, and centrifuging to obtain a precipitate 1; taking the precipitate 1, adding a sodium chloride solution, stirring and crushing, and centrifuging to obtain a precipitate 2; (2) taking the precipitate 2, adding a NaOH solution, stirring, centrifuging to obtain a supernatant, adjusting the pH to neutral, and freeze-drying to obtain eel protein powder. The prepared eel protein powder has the advantages of fast dissolving speed, high dissolving rate, retained protein structure, excellent emulsifying capacity and antioxidant activity, and provides an improved direction for development and utilization of eel protein.

Description

Technical Field

[0001] This invention relates to a rapidly reconstituted eel protein powder and its preparation method, belonging to the field of food processing. Background Technology

[0002] Eels, also known as eels, mainly inhabit tropical and temperate waters. They are highly nutritious, rich in high-quality protein and various essential amino acids, making them a valuable aquatic resource. Eels contain up to 37.81% essential amino acids, far exceeding the FAO's adult amino acid standard (27.60%). Furthermore, besides tryptophan, eels also have high amino acid scores for phenylalanine and tyrosine, indicating a good balance of essential amino acids in eel protein. However, the high amount of high-quality lipids in eel meat has led to a neglect of eel protein extraction and application in eel processing products. The high fat content also presents a challenge for eel protein extraction and processing, as conventional fish protein extraction methods cannot be applied to eel protein extraction.

[0003] Currently, alkaline dissolution and acid precipitation, and isoelectric point precipitation are commonly used to extract proteins from fish meat. Patent CN107151686A discloses the use of isoelectric point precipitation technology to separate cod protein, improving its solubility, emulsifying ability, and foaming properties. Patent CN117567585A discloses the use of alkaline dissolution and acid precipitation to separate tilapia protein, significantly improving the solubility of tilapia protein isolate. The literature "Extraction, Characterization and Utilization of Fish Protein Concentrate; doi.org / 10.4194 / AFS47" discloses the use of organic solvents to extract fish protein, with the protein extracted using isopropanol exhibiting excellent solubility (84.40%), emulsifying ability (68.53%), and emulsifying stability (60.92%).

[0004] However, the aforementioned techniques, such as alkali dissolution and acid precipitation, organic solvents, and the addition of surfactants as traditional methods for protein extraction, suffer from problems such as disrupting the natural conformation of proteins and resulting in low protein concentrations. Furthermore, eel muscle fibers are characterized by small muscle groups and susceptibility to putrefaction. This makes eel proteins prepared using conventional methods difficult to reconstitute after freeze-drying, resulting in unusable flocculent precipitates, which significantly limits the development and application of eel proteins.

[0005] Therefore, there is an urgent need to find a method to prepare eel protein that is easy to reconstitute, can be reconstituted quickly, and has excellent protein properties such as emulsification and emulsion stability. This would help the effective utilization and deep processing of eel resources and has extremely high economic and practical value. Summary of the Invention

[0006] [Technical Issues]

[0007] Eel protein is difficult to extract. Eel protein powder prepared using conventional fish protein extraction methods often has flocculent precipitates, low solubility, and difficulty in reconstitution, making it unsuitable for deep processing.

[0008] [Technical Solution]

[0009] To address the aforementioned problems, this invention provides an eel protein powder with high solubility and rapid reconstitution, as well as a method for its preparation. This invention employs extraction with sodium hydroxide solution and freeze-drying to prepare a rapidly soluble eel protein powder. The prepared eel protein powder exhibits higher solubility and emulsifying properties, providing an improved direction for the development and utilization of eel protein.

[0010] The first objective of this invention is to provide a method for preparing rapidly reconstituted eel protein powder, comprising the following steps:

[0011] (1) Pretreatment: Thaw the eel, remove the skin, stir and crush to obtain eel powder; add phosphate buffer, stir and crush, centrifuge to obtain precipitate 1; take precipitate 1, add sodium chloride solution, stir and crush, centrifuge to obtain precipitate 2;

[0012] (2) Take precipitate 2, add NaOH solution, stir, centrifuge to obtain supernatant, adjust pH to neutral, freeze dry to obtain eel protein powder.

[0013] In one embodiment, the processing temperature of each sample is controlled at 4℃±0.5℃ in steps (1) and (2).

[0014] In one embodiment, the centrifugation in steps (1) and (2) is 6000-10000 rpm for 5-20 min.

[0015] In one embodiment, the concentration of the phosphate buffer in step (1) is 0.01-0.08 mol / L, the pH is 7-8, and the solvent is water; the ratio of eel pulverizer to phosphate buffer is 1 kg: 5-10 L.

[0016] In one embodiment, the concentration of the sodium chloride solution in step (1) is 0.4 to 0.6 mol / L, and the solvent is water; the ratio of precipitate 1 to sodium chloride solution is 1 kg: 5 to 10 L.

[0017] In one embodiment, the eel pulverizer preparation in step (1) is stirred and crushed at 150-650 rpm for 1-5 minutes; optionally, the stirring and crushing process is divided into 4 times.

[0018] In one embodiment, the stirring and crushing in step (1) of precipitate preparation is carried out at 3000-10000 rpm for 1-5 min; optionally, the stirring and crushing process is divided into 4 times.

[0019] In one embodiment, the stirring and crushing in step (1) of the preparation of precipitate 2 is carried out at 3000-10000 rpm for 1-5 min; optionally, the stirring and crushing process is divided into 4 times.

[0020] In one embodiment, the concentration of the NaOH solution in step (2) is 0.08 to 0.12 mol / L, and the solvent is water; the ratio of precipitate 2 to NaOH solution is 1 kg: 8 to 12 L.

[0021] In one embodiment, the stirring in step (2) is carried out at 100-500 rpm for 2 to 16 hours.

[0022] In one embodiment, adjusting the pH to neutral in step (2) means adjusting the pH to 7, and the pH adjuster used is hydrochloric acid solution with a concentration of 0.1 to 1 mol / L.

[0023] In one embodiment, the freeze-drying in step (2) is freeze-drying at -20 to -50°C for more than 20 hours.

[0024] A second objective of this invention is to provide eel protein powder prepared by any of the above methods.

[0025] A third objective of this invention is to provide the application of the above-mentioned eel protein powder in the preparation of products, including but not limited to food, health products, cosmetics, or additives.

[0026] In one embodiment, the food includes, but is not limited to, processed grain products, condiments, meat products, dairy products, beverages, convenience foods, biscuits, canned goods, frozen drinks, quick-frozen foods, potato or puffed foods; the additives include, but are not limited to, emulsifiers, fillers, stabilizers, flavor enhancers, nutritional fortifiers, antioxidants, thickeners or flavorings; the health products include, but are not limited to, antioxidant products; the cosmetics include, but are not limited to: toners, makeup removers, serums, perfumes, cleansing oils, hair oils, body lotions, shower gels, sunscreen gels, facial cleansing gels, face creams, sunscreens, eyeshadows, pressed powders, lipsticks, eyebrow pencils, hair waxes, hairsprays, mousses or sprays.

[0027] A fourth objective of this invention is to provide a product containing the eel protein powder described above; the product includes, but is not limited to, food, health products, cosmetics, or additives.

[0028] In one embodiment, the food includes, but is not limited to, processed grain products, condiments, meat products, dairy products, beverages, convenience foods, biscuits, canned goods, frozen drinks, quick-frozen foods, potato or puffed foods; the additives include, but are not limited to, emulsifiers, fillers, stabilizers, flavor enhancers, nutritional fortifiers, antioxidants, thickeners or flavorings; the health products include, but are not limited to, antioxidant products; the cosmetics include, but are not limited to: toners, makeup removers, serums, perfumes, cleansing oils, hair oils, body lotions, shower gels, sunscreen gels, facial cleansing gels, face creams, sunscreens, eyeshadows, pressed powders, lipsticks, eyebrow pencils, hair waxes, hairsprays, mousses or sprays.

[0029] A fifth objective of this invention is to provide a method for simultaneously improving the performance of eel protein, wherein the performance includes one or more of solubility, emulsifiability, emulsion stability, and antioxidant properties; the method includes the following steps:

[0030] (1) Pretreatment: Thaw the eel, remove the skin, stir and crush to obtain eel powder; add phosphate buffer, stir and crush, centrifuge to obtain precipitate 1; take precipitate 1, add sodium chloride solution, stir and crush, centrifuge to obtain precipitate 2;

[0031] (2) Take precipitate 2, add NaOH solution, stir, centrifuge to obtain supernatant, adjust pH to neutral, freeze dry to obtain eel protein powder with improved performance.

[0032] In one embodiment, in step (1), the concentration of the phosphate buffer is 0.01–0.08 mol / L, the solvent is water, and the pH is 7–8; the ratio of eel pulverizer to phosphate buffer is 1 kg: 5–10 L; the concentration of sodium chloride solution is 0.4–0.6 mol / L, and the solvent is water; the ratio of precipitate 1 to sodium chloride solution is 1 kg: 5–10 L; in step (2), the concentration of NaOH solution is 0.08–0.12 mol / L; the ratio of precipitate 2 to NaOH solution is 1 kg: 8–12 L.

[0033] [Technical Effects]

[0034] This invention provides an eel protein powder with high solubility and rapid reconstitution, and its preparation method. The invention uses sodium hydroxide solution extraction and freeze-drying to prepare a rapidly soluble eel protein powder. This eel protein powder exhibits good solubility and emulsifying properties, providing an improved direction for the development and utilization of eel protein.

[0035] Specifically:

[0036] (1) The method for preparing eel protein powder in this invention simplifies the extraction steps and the formula of the extraction solution, which is of practical significance. Moreover, the eel protein powder obtained has a fast reconstitution speed (dissolves instantly upon adding water) and high solubility (up to 95.31%), thus providing an effective method for storing and applying eel protein.

[0037] (2) The eel protein powder prepared by this invention retains the natural conformation of the protein and has good emulsifying properties (up to 15723.54m). 2 The high levels of eel protein ( / g) and emulsification stability (176.50%) provide a foundation for its development and use.

[0038] (3) The eel protein powder prepared by this invention has excellent antioxidant activity, and its ABTS + • The free radical scavenging rate can reach up to 92.46%, providing a foundation for the development and use of antioxidant products. Attached Figure Description

[0039] Figure 1 The results show the solubility and dissolution rate of eel protein powder.

[0040] Figure 2 The results of the tests on the emulsifying properties and emulsion stability of eel protein powder;

[0041] Figure 3 ABTS eel protein powder + • Test results of free radical scavenging rate. Detailed Implementation

[0042] The following examples and comparative examples further illustrate this application in detail. Unless otherwise specified, all raw materials used in this application are commercially available.

[0043] Raw materials used in the examples and comparative examples:

[0044] The eels were purchased from Guangxi Zhongnan Fishery Co., Ltd.

[0045] Unless otherwise specified, the solvent used in the solutions mentioned in the examples is water; unless otherwise specified, the percentages refer to mass percentages; lyophilization is lyophilization at -48°C for 48 hours.

[0046] Example 1

[0047] A method for preparing rapidly resoluble eel protein powder includes the following steps:

[0048] (1) Thaw the eel, remove the skin, stir and crush at 150 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), add 0.02 mol / L phosphate buffer (pH 7.5) at a ratio of 1 kg: 8 L, stir and crush at 3000 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), centrifuge at 6000 rpm for 5 min to obtain precipitate 1; take precipitate 1, add 0.5 mol / L sodium chloride solution at a ratio of 1 kg: 8 L, stir and crush at 3000 rpm for 1 min, centrifuge at 6000 rpm for 5 min to obtain precipitate 2;

[0049] (2) Take precipitate 2 and add 0.1 mol / L NaOH solution (pH 13) at a ratio of 1 kg: 10 L. Stir at 150 rpm for 2 h; centrifuge at 6000 rpm for 5 min, collect the supernatant, adjust the pH to 7 with 1 mol / L hydrochloric acid solution (no precipitation will occur during this period), and freeze-dry the supernatant to obtain eel protein powder.

[0050] The sample temperature was controlled at 4℃±0.5℃ throughout the process.

[0051] Example 2

[0052] A method for preparing rapidly resoluble eel protein powder includes the following steps;

[0053] (1) Thaw the eel, remove the skin, stir and crush at 150 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), add 0.08 mol / L phosphate buffer (pH 7.5) at a ratio of 1 kg: 5 L, stir and crush at 5000 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), centrifuge at 6000 rpm for 5 min to obtain precipitate 1; take precipitate 1, add 0.6 mol / L sodium chloride solution at a ratio of 1 kg: 5 L, stir and crush at 10000 rpm for 1 min, centrifuge at 6000 rpm for 5 min to obtain precipitate 2;

[0054] (2) Take precipitate 2 and add 0.08 mol / L NaOH solution (pH 13) at a ratio of 1 kg: 12 L. Stir at 150 rpm for 2 h; centrifuge at 6000 rpm for 5 min, collect the supernatant, adjust the pH to 7 with 1 mol / L hydrochloric acid solution, and freeze dry to obtain eel protein powder.

[0055] The sample temperature was controlled at 4℃±0.5℃ throughout the process.

[0056] Example 3

[0057] A method for preparing rapidly resoluble eel protein powder includes the following steps;

[0058] (1) Thaw the eel, remove the skin, stir and crush at 650 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), add 0.01 mol / L phosphate buffer (pH 7.5) at a ratio of 1 kg: 10 L, stir and crush at 10000 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), centrifuge at 6000 rpm for 5 min to obtain precipitate 1; take precipitate 1, add 0.4 mol / L sodium chloride solution at a ratio of 1 kg: 10 L, stir and crush at 10000 rpm for 1 min, centrifuge at 6000 rpm for 5 min to obtain precipitate 2;

[0059] (2) Take precipitate 2 and add 0.12 mol / L NaOH solution (pH 13) at a ratio of 1 kg: 8 L. Stir at 150 rpm for 2 h; centrifuge at 6000 rpm for 5 min, collect the supernatant, adjust the pH to 7 with 1 mol / L hydrochloric acid solution, and freeze dry to obtain eel protein powder.

[0060] The sample temperature was controlled at 4℃±0.5℃ throughout the process.

[0061] Comparative Example 1

[0062] The preparation of eel protein powder using acid precipitation includes the following steps:

[0063] (1) Thaw the eel, remove the skin, stir and crush at 150 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), add the extraction solution at a ratio of 1 kg: 8 L, stir and crush at 3000 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), centrifuge at 6000 rpm for 5 min to obtain a precipitate; take the precipitate, add 0.5 mol / L sodium chloride solution at a ratio of 1 kg: 8 L to resuspend the precipitate, filter with gauze to remove insoluble matter, and obtain eel protein suspension;

[0064] (2) Take the eel protein suspension, adjust the pH to 6.25 with 1 mol / L hydrochloric acid solution, centrifuge at 6000 rpm for 5 min to obtain a paste precipitate, freeze dry to obtain eel protein powder.

[0065] The sample temperature was controlled at 4℃±0.5℃ throughout the process.

[0066] Comparative Example 2

[0067] The preparation of eel protein powder using surfactants includes the following steps:

[0068] (1) Thaw the eel, remove the skin, stir and crush at 150 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), mix with biological buffer No. 1 (sodium chloride 1.48 g / L, tris(hydroxymethyl)aminomethane 0.62 g / L, disodium ethylenediaminetetraacetate 1.88 g / L, the rest is water) at a ratio of 1 kg: 10 L, stir and crush at 3000 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), centrifuge at 6000 rpm for 5 min to obtain precipitate 1;

[0069] (2) Take precipitate 1 and mix it with biological buffer No. 2 (sodium chloride 5.9g / L, tris(hydroxymethyl)aminomethane 0.62g / L, disodium ethylenediaminetetraacetate 1.88g / L, the remainder being water) at a ratio of 1kg:10L to obtain a mixture; add surfactant Triton X100 until the final concentration in the mixture is 0.4%, stir and crush at 3000rpm for 1min, and centrifuge at 6000rpm for 5min to obtain precipitate 2;

[0070] (3) Take precipitate 2 and mix it with biological buffer No. 2 (sodium chloride 5.9g / L, tris(hydroxymethyl)aminomethane 0.62g / L, disodium ethylenediaminetetraacetate 1.88g / L, the remainder being water) at a ratio of 1kg:5L. Stir and crush at 3000rpm for 1min (the crushing process in 1min needs to be divided into 4 times), and centrifuge at 6000rpm for 5min to obtain precipitate 3.

[0071] (4) Take precipitate 3 and mix it with biological buffer No. 3 (sodium chloride 0.14g / L, tris(hydroxymethyl)aminomethane 0.62g / L, the remainder being water) at a ratio of 1kg:10L. Stir and crush at 3000rpm for 1min (the crushing process in 1min needs to be divided into 4 times), centrifuge at 6000rpm for 5min to obtain a paste-like precipitate, and freeze-dry to obtain eel protein powder.

[0072] Throughout the process, the sample temperature was controlled at 4℃±0.5℃.

[0073] Comparative Example 3

[0074] The preparation of eel protein powder using the alkali dissolution and acid precipitation method includes the following steps:

[0075] (1) Thaw the eel, remove the skin, stir and crush at 150 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), mix with 2 mol / L sodium hydroxide solution at a ratio of 1 kg: 8 L, stir and crush at 3000 rpm for 1 min (the crushing process in 1 min needs to be divided into 4 times), stir at 450 rpm for 2 h, centrifuge at 6000 rpm for 5 min, and collect the supernatant.

[0076] (2) Adjust the pH of the supernatant to 5 with 1 mol / L hydrochloric acid solution, centrifuge at 6000 rpm for 5 min, take the precipitate and disperse it in deionized water, adjust the pH to 7 with 1 mol / L sodium hydroxide solution, and freeze dry to obtain eel protein powder.

[0077] Comparative Example 4

[0078] The preparation of eel protein powder using alcohol extraction includes the following steps:

[0079] Thaw the eel, remove the skin, add acetone of equal mass to the eel meat, stir at 150 rpm for 45 min; filter to obtain precipitate 1, dry; add 90% ethanol solution of equal mass to the precipitate, reflux for 45 min, filter to obtain precipitate 2, dry; wash precipitate 2 with ethanol, filter, dry, pulverize to obtain eel protein powder.

[0080] Comparative Example 5

[0081] Based on Example 1, the 0.02 mol / L phosphate buffer in step (1) was changed to an extraction solution (sodium phosphate 1.69 g / L, sodium chloride 5.86 g / L, magnesium chloride 0.19 g / L, ethylene glycol diethyl ether diaminetetraacetic acid 0.38 g / L, and the remainder was water). The remaining steps were the same as in Example 1, and eel protein powder was prepared.

[0082] Comparative Example 6

[0083] Based on Example 1, the 0.5 mol / L sodium chloride solution in step (1) was changed to a 0.1 mol / L sodium chloride solution, and the remaining steps were the same as in Example 1, to prepare eel protein powder.

[0084] Example 4: Performance Testing of Eel Protein Powder

[0085] The eel protein powders prepared in Examples 1-3 and Comparative Examples 1-6 were tested for their dissolution rate, solubility, and emulsifying ability. The testing steps are as follows:

[0086] 1. Dissolution rate detection:

[0087] Add 0.02g of protein powder to 10mL of deionized water and observe the dissolution at 0, 10, 30 and 60 minutes after dissolution, and observe whether there is any flocculent precipitate.

[0088] 2. Solubility test:

[0089] The eel protein powder prepared in the comparative example and the example were mixed with deionized water at a ratio of 0.2 mg: 1 mL to obtain the original solution. The original solution was placed at 4°C, stirred at 150 rpm for 30 min, and then centrifuged at 8000 rpm for 15 min. The protein concentration in the supernatant was determined by BCA method, and the protein solubility (%) was calculated.

[0090] The specific calculation formula is as follows:

[0091] Solubility = (protein content in supernatant / protein content in original solution) × 100%.

[0092] 3. Emulsifying ability:

[0093] Take the eel protein powder prepared in the comparative example and the embodiment, and prepare a 9 mL eel protein solution with a concentration of 0.5 mg / mL using deionized water. Add 3 mL soybean oil and shear at 10000 rpm for 1 min. Take 50 μL of sample from the bottom of the sample emulsion at 0 min and 10 min after shearing, respectively, add 5 mL of 0.1% (w / v, mg / mL) SDS solution and mix thoroughly. Measure the absorbance at 500 nm.

[0094] Emulsificability is represented by the Emulsificability Index (EAI), and emulsification stability (ES) is represented by the Emulsification Stability Index. Both the Emulsificability Index and Emulsification Stability are calculated using the following formulas:

[0095] Emulsifying property calculation formula:

[0096] EAI=(2.303×2×OD 500 ×Dilution rate) / [Sample mass concentration × (1 - oil phase fraction) / 100]

[0097] Emulsion stability calculation formula:

[0098] ES = 100 × EAI 0min / (EAI 0min -EAI 10min ).

[0099] 4. Antioxidant properties:

[0100] The eel protein obtained from the examples and comparative examples was used to prepare an eel protein solution with a concentration of 0.5 mg / mL using deionized water, which was then used as the sample to be tested.

[0101] Accurately weigh 0.0960 g ABTS and 0.0116 g potassium persulfate, dissolve them in deionized water and dilute to a final volume in a 25 mL brown volumetric flask. Wrap the flask with aluminum foil, shake well, and react at room temperature in the dark for 12 hours to form ABTS. + • Stock solution.

[0102] Dilute ABTS with 10 mmol / L phosphate buffer (pH 7.4) before use. + • The stock solution was prepared so that its absorbance at 734 nm was 0.700 (±0.020), thus obtaining ABTS. + • Working fluid.

[0103] Accurately pipette 10 μL of the sample to be tested and mix it with 990 μL of ABTS. + • Mix the working solution thoroughly. After reacting in the dark for 10 min, measure the absorbance at 734 nm, using a 70% ethanol solution as a blank control.

[0104] Antioxidant properties with ABTS + • Free radical scavenging ability is expressed in ABTS + The formula for calculating the free radical scavenging rate is as follows:

[0105] ABTS + • Free radical scavenging rate (%) = (A 空白 -A 样品 ) / A 空白 ×100%.

[0106] The test results of the eel protein powders prepared in Examples 1-3 and Comparative Examples 1-6 are as follows:

[0107] Table 1 Performance test results of eel protein powder

[0108]

[0109] Solubility details are shown in Table 1 and Figure 1 As shown, the results indicate that the eel protein powder prepared in Examples 1-3 was superior to the comparative examples in terms of solubility at 0, 10, 30, and 60 min. The eel protein powder prepared in Examples 1-3 dissolved rapidly after being added to water and had stable solubility. In contrast, the eel protein powder prepared in Examples 1-6 was difficult to dissolve and the solubility did not improve with dissolution time.

[0110] The solubility results are shown in Table 1. The results show that the solubility of the eel protein powder prepared in Examples 1-3 is higher than that of the comparative examples, and is increased by more than 73.91%, 7.89%, 74.90%, 76.10%, 13.86%, and 33.89% respectively compared with the comparative examples 1-6.

[0111] The results of emulsifying properties and emulsion stability are shown in Table 1 and Figure 2 As shown, the results indicate that the eel protein powder prepared in Examples 1-3 has slightly lower emulsification performance than Comparative Example 1, but is 185%, 139%, 1031%, 1135%, and 781% higher than Comparative Examples 2-6, respectively. The emulsification stability results of Examples 1-3 are similar to those of Comparative Examples 1-6. Among them, Comparative Example 1 has high emulsification but low solubility, and Comparative Example 2 has high emulsification stability but low emulsification. Their overall potential for preparing emulsions is not as good as that of the Examples.

[0112] ABTS + The results of free radical scavenging are shown in Table 1 and... Figure 3As shown, the results indicate that the eel proteins prepared in Examples 1-3 possess the highest ABTS free radical scavenging rate. + The free radical scavenging rate was increased by more than 336%, 817%, 353%, 934%, 397%, and 359% respectively compared with Comparative Examples 1 to 6, indicating that the eel protein powder prepared in the examples has excellent antioxidant properties.

[0113] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims.

Claims

1. A method for preparing rapidly reconstituted eel protein powder, characterized in that, Includes the following steps: (1) Pretreatment: Thaw the eel, remove the skin, stir and crush to obtain eel powder; add phosphate buffer, stir and crush, centrifuge to obtain precipitate 1; take precipitate 1, add sodium chloride solution with a concentration of 0.4~0.6 mol / L, stir and crush, centrifuge to obtain precipitate 2; (2) Take precipitate 2, add NaOH solution, stir, centrifuge to obtain supernatant, adjust pH to neutral, freeze dry to obtain eel protein powder.

2. The preparation method according to claim 1, characterized in that, In step (1), the concentration of phosphate buffer is 0.01~0.08 mol / L and the pH is 7~8; the ratio of eel pulverized material to phosphate buffer is 1 kg: 5~10 L.

3. The preparation method according to claim 1, characterized in that, In step (1), the ratio of precipitate 1 to sodium chloride solution is 1 kg: 5~10 L.

4. The preparation method according to claim 1, characterized in that, In step (2), the concentration of NaOH solution is 0.08~0.12 mol / L; the ratio of precipitate 2 to NaOH solution is 1 kg: 8~12 L.

5. The preparation method according to claim 1, characterized in that, In steps (1) and (2), the processing temperature of each sample is controlled at 4℃±0.5℃.

6. Eel protein powder prepared by any one of the preparation methods according to claims 1 to 5.

7. The application of the eel protein powder according to claim 6 in the preparation of the product, characterized in that, The products include food, cosmetics, or additives.

8. The application according to claim 7, characterized in that, The food products include grain products, condiments, meat products, dairy products, beverages, biscuits, canned goods, frozen drinks, quick-frozen foods, and potato or puffed foods; the additives include emulsifiers, fillers, stabilizers, flavor enhancers, nutritional fortifiers, antioxidants, thickeners, or fragrances; the cosmetics include: toners, makeup removers, serums, perfumes, makeup remover oils, hair oils, body lotions, shower gels, sunscreen gels, facial cleansing gels, face creams, sunscreens, eyeshadows, pressed powders, lipsticks, eyebrow pencils, hair waxes, hairsprays, mousses, or sprays.

9. The use of the eel protein powder according to claim 6 in the preparation of health products.

10. The application according to claim 9, characterized in that, The health supplement in question is an antioxidant product.

11. A product characterized in that, The product contains the eel protein powder as described in claim 6; the product includes food, cosmetics, or additives.

12. A health product, characterized in that, The health product contains the eel protein powder as described in claim 6.

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