An anti-wrinkle and firming composition containing plant extracts, and preparation method and application thereof

Abstract

The present invention relates to an anti-wrinkle and firming efficacy composition containing plant extracts and its preparation method and application, and belongs to the technical field of skin care products. In the composition provided by the present invention, when a small amount of Bossain is used together with golden button flower extract, aloe extract, seaweed extract and acerola cherry extract, the seaweed extract and acerola cherry extract are combined with Bossain to enhance the antioxidant capacity of the skin, resist the invasion of the external environment, and promote the synthesis of collagen and skin repair; the seaweed extract, aloe extract and Bossain are combined to enhance the antioxidant capacity of the skin and improve skin elasticity; the golden button flower extract contains rich N-alkylamide components, and a small amount of Bossain can play a good anti-wrinkle effect. The components cooperate with each other, so that Bossain still shows a good anti-wrinkle and firming effect when the dosage is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and in particular, relates to an anti-wrinkle and firming composition containing plant extracts, and a preparation method and application thereof. Background Art

[0002] The English name of "Pro-Xylane" is "Pro-Xylane", which is a xylose derivative with anti-aging active ingredients. Pro-Xylane is a mixture of glycoproteins derived from xylose. Since xylose exists in large quantities in beech trees and has the ability to promote the production of glucosaminoglycans, also known as mucopolysaccharides (GAGs), Pro-Xylane, extracted from beech trees, has similar effects to xylose. Since "Pro-Xylane" can promote the synthesis of glycosaminoglycans and increase the production of dermal collagen, it can promote the synthesis of collagen, thereby promoting dermal repair, improving skin elasticity, improving fine lines, increasing skin firmness and preventing aging, thereby playing an anti-aging role on the skin. However, the cost of Pro-Xylane is relatively high, and the speed of effectiveness needs to be improved. Summary of the invention

[0003] The object of the present invention is to provide an anti-wrinkle and firming composition containing plant extracts, which reduces the amount of phosphatidylcholine used and still has good anti-wrinkle and firming effects.

[0004] The second object of the present invention is to provide a method for preparing the above composition.

[0005] The third object of the present invention is to provide application of the above composition in skin care products.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] The method for preparing the anti-wrinkle and firming composition containing plant extracts of the present invention is specifically to uniformly mix the following components in parts by weight:

[0008]

[0009]

[0010] As a preferred embodiment of the present invention, the composition comprises the following components in parts by weight:

[0011]

[0012] As a preferred embodiment of the present invention, the composition comprises the following components in parts by weight:

[0013]

[0014] As a preferred embodiment of the present invention, the extract of golden button flower is prepared by the following operation: crushing the golden button flower, and performing ultrasonic extraction with cyclohexane to prepare the golden button flower extract.

[0015] As a preferred embodiment of the present invention, the seaweed extract is prepared by the following operation: extracting seaweed with ethanol to obtain the seaweed extract.

[0016] As a preferred embodiment of the present invention, the acerola cherry extract is prepared by the following operations:

[0017] Preparation of acerola cherry pulp: taking acerola cherry fruit as a raw material, crushing it by wet ultrafine grinding method to obtain acerola cherry pulp;

[0018] Preparation of acerola cherry extract: Acerola cherry pulp was extracted with an aqueous two-phase solution formed by (NH4)2SO4 and ethanol.

[0019] As a preferred embodiment of the present invention, in the preparation of acerola cherry pulp, the wet ultrafine grinding method is specifically: mixing acerola cherry and water in a mass ratio of 1 to 2:3 and crushing.

[0020] As a preferred embodiment of the present invention, the specific parameters for preparing acerola cherry extract are: extraction is performed with 1.66 g of (NH4)2SO4 per 10 mL of the extraction system, 4.3 mL of ethanol, a temperature of 40°C, and an extraction time of 20 min.

[0021] The composition prepared by the above preparation method.

[0022] The composition is used as an effective component in skin care products.

[0023] Beneficial effects of the present invention:

[0024] The composition provided by the present invention comprises bosaicin, golden button flower extract, aloe extract, seaweed extract and acerola extract. The aloe extract can quickly penetrate into the deep layer of the skin and provide lasting moisture nourishment for the skin; the acerola extract not only provides rich vitamin C for the skin, promotes the synthesis of collagen and the regeneration of skin cells, but also has certain antioxidant and anti-inflammatory effects. When the acerola extract is used in combination with the aloe extract, it can play a comprehensive skin care effect and has a stronger moisturizing effect; and when the acerola extract is used in combination with the seaweed extract, the seaweed extract enhances the effect of vitamin C in the acerola extract in promoting the synthesis of collagen and the regeneration of skin cells.

[0025] When a small amount of posione is used together with golden button flower extract, aloe extract, seaweed extract and acerola cherry extract, the combination of seaweed extract and acerola cherry extract with posione can enhance the skin's antioxidant capacity, resist the damage of the external environment, and promote the synthesis of collagen and skin repair; the combination of seaweed extract, aloe extract and posione can enhance the skin's antioxidant capacity and improve skin elasticity; golden button flower extract contains rich N-alkylamide components, which can play a good anti-wrinkle effect with a small amount of posione. The mutual cooperation between the various components makes posione still show a good anti-wrinkle and firming effect when the dosage is reduced.

[0026] Furthermore, in the preparation method of the acerola cherry extract of the present invention, a wet ultrafine grinding method + a two-phase aqueous extraction method is used, and the obtained acerola cherry extract contains high active ingredients and performs well in efficacy tests. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with specific implementation methods.

[0028] The aloe extract described below is aloe vera freeze-dried powder, batch number 20150501, which will not be described in detail.

[0029] Example 1

[0030] Preparation of Spilanthes japonica flower extract

[0031] 80 kg of golden button flowers were crushed and ultrasonically extracted with cyclohexane for 30 minutes to obtain an extract with a solid-liquid ratio of 1:20. The extract was decompressed and the solvent was recovered to obtain the golden button flower extract.

[0032] Example 2

[0033] Preparation of seaweed extract

[0034] 80 kg of seaweed was soaked and extracted three times with 25% ethanol solution, each extraction time was 7 days, the filtrates were combined, concentrated to an extract, and finally made into a freeze-dried powder seaweed extract.

[0035] Example 3

[0036] Preparation of Acerola Extract

[0037] Preparation of acerola cherry pulp: taking 100 kg of acerola cherry fruit as a raw material, mixing acerola cherry and water in a mass ratio of 1 to 2:3, and crushing to obtain acerola cherry pulp;

[0038] Preparation of acerola cherry extract: The acerola cherry pulp is ground again with a wet ultrafine grinder, the grinding frequency is controlled to 30 Hz, and the grinding time is adjusted to 40 s to obtain a pre-made slurry. The pre-made slurry is extracted with a two-phase aqueous solution formed by (NH4)2SO4 and ethanol, with an extraction system of 1.66 g (NH4)2SO4, 4.3 mL ethanol, a temperature of 40°C, and an extraction time of 20 min per 10 mL of the extraction system. The two phases after extraction are evaporated and concentrated separately, the solvent is removed, and the acerola cherry extract is obtained by mixing.

[0039] Example 4

[0040] The extract of golden button flower, seaweed extract, acerola extract, aloe extract and bosacein prepared in Examples 1 to 3 were mixed according to the following weights to prepare a composition:

[0041]

[0042]

[0043] Example 5

[0044] The extract of golden button flower, seaweed extract, acerola extract, aloe extract and bosacein prepared in Examples 1 to 3 were mixed according to the following weights to prepare a composition:

[0045]

[0046] Example 6

[0047] The extract of golden button flower, seaweed extract, acerola extract, aloe extract and bosacein prepared in Examples 1 to 3 were mixed according to the following weights to prepare a composition:

[0048]

[0049] Example 7

[0050] The extract of golden button flower, seaweed extract, acerola extract, aloe extract and bosacein prepared in Examples 1 to 3 were mixed according to the following weights to prepare a composition:

[0051]

[0052]

[0053] Example 8

[0054] The extract of golden button flower, seaweed extract, acerola extract, aloe extract and bosacein prepared in Examples 1 to 3 were mixed according to the following weights to prepare a composition:

[0055]

[0056] Comparative Example 1

[0057] Compared with Example 6, the golden button flower extract was not added, and an equal mass of acerola cherry extract was used instead. The rest was the same as Example 6.

[0058] Comparative Example 2

[0059] Compared with Example 6, aloe vera extract was not added and seaweed extract of equal mass was used instead. The rest was the same as Example 6.

[0060] Comparative Example 3

[0061] Compared with Example 6, no seaweed extract was added, and an equal mass of golden button flower extract was used instead. The rest was the same as Example 6.

[0062] Comparative Example 4

[0063] Compared with Example 6, the acerola cherry extract was not added and an equal mass of aloe vera extract was used instead. The rest was the same as Example 6.

[0064] The compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4 were tested as follows:

[0065] Test Example 1 Fibroblast Anti-wrinkle Index Test

[0066] The fiber formation was performed by setting a blank group and a sample group, and the sample group was added with the compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4, respectively. The number of cells per well was controlled to be 5×10 5 After culturing for 24 h in a 37°C, 5% CO2 incubator, the culture medium was discarded and a small amount of PBS (pH = 7.4) was added to just cover the cells. UVA was used to stimulate the cells, and the UV radiation dose was 7 J / cm 2 , the blank group was not irradiated. PBS was discarded, and samples of different concentrations were added. The concentration of the composition was 6%, VC was 80 μg / mL, and GSH was 20 μm / mL to stimulate the cells for 24 hours. The cell culture supernatant was centrifuged at 4°C, 1000g for 15 minutes, and the supernatant was taken. The human matrix metalloproteinase 1 (MMP-1) ELISAKit was used to test the MMP-1 content. The cell suspension was diluted with PBS (pH 7.2-7.4) to a cell concentration of 1×10 6 The cells were broken by repeated freezing and thawing and centrifuged at 1000 g for 20 min. The supernatant was collected and the type I collagen (CoL I) test kit was used to test the CoL I content. The human hyaluronic acid (HA) ELISA Kit was used to test the HA content. The operations were carried out according to the instructions of the kits. The results are shown in Table 1.

[0067] Table 1

[0068]

[0069] As shown in Table 1, the MMP-1 contents measured in the compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4 are all less than 22.88 ng / mL of the blank group, indicating that the compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4 can reduce the expression of MMP-1 in fibroblasts. The MMP-1 contents measured in the compositions prepared in Comparative Examples 1 to 4 are all greater than those in Examples 4 to 8 to varying degrees, indicating that Comparative Examples 1 to 4 have different degrees of reduction in the effect of reducing the expression of MMP-1 in fibroblasts.

[0070] The measured Co L I contents of the compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4 were all greater than 5.6 ng / mL of the blank group, indicating that the compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4 could increase the expression of CoLI. The measured Co L I contents of the compositions prepared in Comparative Examples 1 to 4 were all less than those in Examples 4 to 8 to varying degrees, indicating that the effects of Comparative Examples 1 to 4 on increasing the expression of CoLI were reduced to varying degrees.

[0071] The HA contents measured in the compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4 were all greater than 5.3 ng / mL of the blank group, indicating that the compositions prepared in Examples 4 to 8 and Comparative Examples 1 to 4 were able to increase the HA content of fibroblasts and reverse the reduction of HA caused by UVA radiation. The HA contents measured in the compositions prepared in Comparative Examples 1 to 4 were all less than those in Examples 4 to 8 to varying degrees, indicating that Comparative Examples 1 to 4 had varying degrees of reduction in the effect of reversing the reduction of HA caused by UVA radiation.

[0072] The compositions obtained in Examples 4 to 8 and Comparative Examples 1 to 4 were made into eye creams according to the following operation, and then human trial experiments were carried out:

[0073] (1) Pre-dispersing carbomer in water, adding other raw materials of phase B after evenly dispersing, placing in a water bath, controlling the temperature at 75-80° C., and stirring at a constant speed for about 30 minutes until all the carbomer is dissolved, to obtain a mixture B;

[0074] (2) Weigh the raw material of phase A into a beaker, place it in a water bath, control the temperature at 75-80°C, and stir at a constant speed for about 30 minutes to obtain oily material A after all the raw materials are dissolved;

[0075] (3) Premixing and stirring the raw materials of phase C to obtain mixed material C;

[0076] (4) adding the phase D raw material to water to prepare a 2 wt % water mixture as mixed material D;

[0077] (5) Pour mixed material B into A, control the temperature within the range of 75-80°C and stir rapidly for 30 minutes, then reduce the temperature to 40-45°C, add mixed materials C and D, and then reduce the temperature to 30-35°C, stop stirring and discharge the materials to obtain an eye cream.

[0078] Table 2

[0079]

[0080] Test Example 2 Human Trial Test

[0081] The eye creams prepared in Examples 4 to 8 and Comparative Examples 1 to 4 were used for human body experiments, and the specific operation was as follows:

[0082] Volunteer inclusion criteria: ① healthy Chinese female subjects aged 34 to 65 years; ② "crow's feet" level ≥ 2 points, using the standard in "Ski n Ageing Atlas Volume 2 Asian Type"; ③ not participating in other skin care products or clinical research within 1 month; ④ signing the informed consent form.

[0083] Exclusion criteria: ① Women who are planning to become pregnant, pregnant, breastfeeding, or within 6 months after giving birth; ② Those with serious systemic diseases, immunodeficiency, or autoimmune diseases; ③ Those with allergic diseases or cosmetic allergies in the past 1-2 years; ④ Those who are currently or have used anti-wrinkle products and medical beauty treatments on the test area or participated in other tests in the past month; ⑤ Those who have dermatological diseases in the test area or are receiving medication; ⑥ The subjects have diseases that may affect the test, or are undergoing treatments that may affect the test; ⑦ Those who are considered unsuitable to participate by the researchers.

[0084] The test process test plan is as follows:

[0085] 1. Skin application

[0086] After cleansing, volunteers took an appropriate amount of eye cream and applied it around the eyes. Massage gently until absorbed. Apply once in the morning and evening every day for 30 days. Test once before use and once after 1 month.

[0087] 2. Instrument testing

[0088] (1) Clean your eyes with clean water and sit quietly in the room for at least 15 minutes.

[0089] (2) The elasticity index R2 of the eye before and after use was measured using a skin elasticity meter. The average value was taken for three measurements. The results are shown in Table 3. The R2 value is the ratio of the skin's rebound amount when there is no negative pressure to the maximum stretch amount when there is negative pressure. The closer the R2 value is to 1, the better the skin elasticity.

[0090] (3) The skin glossiness measuring probe was used to measure the glossiness of the eye area before and after use. The average value was taken after three measurements. The results are shown in Table 3. The higher the glossiness value, the better the skin glossiness.

[0091] (4) The moisture of the eye skin before and after use was measured using a skin moisture measuring probe. The average value was taken after 10 measurements. The results are shown in Table 3.

[0092] Table 3

[0093]

[0094] As shown in Table 3, in the human body experiment, the R2 values ​​of Examples 4 to 8 and Comparative Examples 1 to 4 before using the eye cream were all less than 0.62, and the R2 values ​​after use were all greater than 0.62. The eye creams prepared by Examples 4 to 8 and Comparative Examples 1 to 4 can make the R2 value close to 1, indicating that they all have the effect of enhancing skin elasticity. The R2 values ​​of the eye creams prepared by Comparative Examples 1 to 4 after use are all less than those of Examples 4 to 8 to varying degrees, which shows that the effect of Comparative Examples 1 to 4 on enhancing skin elasticity is reduced to varying degrees.

[0095] The moisture content of the eye skin before using the eye cream in Examples 4 to 8 and Comparative Examples 1 to 4 was measured to be less than 64%, and the moisture content of the eye skin after using the eye cream was greater than 64%, indicating that the eye creams prepared in Examples 4 to 8 and Comparative Examples 1 to 4 have the effect of enhancing skin moisture. The moisture content of the eye skin after using the eye cream prepared in Comparative Examples 1 to 4 was measured to be less than that in Examples 4 to 8 to varying degrees, indicating that the efficacy of Comparative Examples 1 to 4 in enhancing skin moisture was reduced to varying degrees.

[0096] The above is only an implementation method of the invention, and does not limit the scope of patent protection. Those skilled in the art can make non-substantial changes or substitutions based on the invention, which still fall within the scope of patent protection.

Claims

1. A method for preparing an anti-wrinkle and firming composition containing plant extracts, characterized in that: Specifically, the following components in parts by weight are mixed uniformly: 1~20 parts of Bose 0.1~5 parts of Spirulina extract Aloe extract 0.1~10 parts 0.1~10 parts of seaweed extract 0.1-10 parts of acerola extract; The extract of golden button flower is prepared by the following operations: crushing the golden button flower, and performing ultrasonic extraction with cyclohexane to prepare the golden button flower extract; The seaweed extract is prepared by the following operations: extracting the seaweed with ethanol to obtain the seaweed extract; The acerola cherry extract is prepared by the following operations: Preparation of acerola cherry pulp: taking acerola cherry fruit as a raw material, crushing it by wet ultrafine grinding method to obtain acerola cherry pulp; Preparation of acerola cherry extract: Acerola cherry pulp was extracted with an aqueous two-phase solution formed by (NH4)2SO4 and ethanol.

2. The preparation method according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 5~10 parts of Bose 0.1~2 parts of golden button flower extract Aloe vera extract 5-10 parts 1~5 parts of seaweed extract 0.1~3 parts of acerola cherry extract.

3. The preparation method according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 5~8 parts of Bose 0.1~0.5 parts of golden button flower extract Aloe vera extract 5-8 parts 1~3 parts of seaweed extract 0.1~1 part of acerola cherry extract.

4. The preparation method according to claim 1, characterized in that: In the preparation of acerola cherry pulp, the wet ultrafine grinding method is specifically as follows: acerola cherry fruit and water are mixed in a mass ratio of 1-2:3 and crushed.

5. The preparation method according to claim 1, characterized in that: The specific parameters for preparing the acerola cherry extract are: extraction with 1.66 g of (NH4)2SO4 per 10 mL of the extraction system, 4.3 mL of ethanol, a temperature of 40°C, and an extraction time of 20 min.

6. A composition obtained by the preparation method according to any one of claims 1 to 5.

7. Use of the composition prepared by the preparation method according to any one of claims 1 to 5 as an effective component in the preparation of skin care products.

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