A method for efficient mixed sowing using artificial seeds

Abstract

The present invention belongs to the technical field of agricultural planting, and specifically relates to a method for efficient mixed sowing using artificial seeds. First, the seed materials to be mixed are mixed in proportion and then soaked in a disinfectant with specific components, and finally the seeds are cleaned and the moisture of the seeds is sucked dry; the obtained sterile seed materials are inoculated on a culture medium for suspension and mixing, and the suspension is dripped into a complexing agent for complexation, and finally the obtained artificial endosperm is frozen and stored, and when used, the refrigerated artificial endosperm is placed in a culture matrix for cultivation and germination. The artificial seed production provided by the present invention is not restricted by seasons, is easy to store and transport, can maintain the excellent properties of grass seeds, can improve seed adaptability, and shorten germination time; the artificial seeds are used to reasonably mix lawn grass seeds, the process is simple, the cost is low, and the lawn effect is good, and it can be widely used in golf courses, greens and other places with high requirements for lawn quality.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a method for efficient mixed sowing using artificial seeds. Background Art

[0002] Mixed seeding is an important way to build a good green lawn. Mixed grass species need to be reasonably matched according to the biological characteristics and functions of lawn grasses and people's needs. For example, mixed seeding of warm-season grass species and cool-season grass species can extend the green period of lawns; mixed seeding of grass species with strong trampling resistance and mowing resistance can improve the wear resistance of lawns, etc. Mixed seeding can bring into play the excellent characteristics of various lawn grasses, adapt to the changing environmental conditions, form lawns faster, maintain the stability of lawn quality, and extend the life of lawns.

[0003] In the prior art, a Chinese patent with publication number CN100348084C provides a method for constructing a skiing lawn. The method comprises mixing bluegrass seeds, tall fescue seeds and ryegrass seeds in proportion, and then rationally and scientifically optimizing the sowing amount of the three grass seeds, so that the skiing lawn has higher wear resistance and recovery ability after being paved, thereby effectively improving the service life of the skiing lawn.

[0004] However, the existing mixed sowing technology mentioned above still has the problem of insufficient pre-treatment of mixed sowing seeds, resulting in insufficient germination rate of mixed sowing seeds and difficulty in storage, which greatly limits the development of mixed sowing technology. Summary of the invention

[0005] In view of the above-mentioned problems existing in the current mixed sowing technology, the present invention provides a method for efficient mixed sowing using artificial seeds, which can greatly improve the storage time and germination rate of artificial seed mixed sowing by double sterilizing the artificial seed materials and culturing the seed materials to the artificial endosperm stage and finally mixing and culturing. The specific technical scheme is as follows:

[0006] First, the present invention provides a method for efficient mixed sowing using artificial seeds, characterized in that it comprises the following steps:

[0007] S1 Seed material disinfection: Mix the seed materials to be mixed in proportion and soak them in disinfectant I. After soaking, wash the seeds and then soak them in disinfectant II. Finally, wash the seeds and absorb the moisture of the seeds;

[0008] S2: preparing artificial endosperm: inoculating the sterile seed material obtained in S1 onto the culture medium, suspending and mixing, and dropping the suspension into the complexing agent for complexation;

[0009] S3 storage and culture: The artificial endosperm obtained in S2 is frozen and stored, and when used, the refrigerated artificial endosperm is placed in a culture matrix to cultivate germination.

[0010] Furthermore, the seed material in step S1 includes common bermudagrass seeds and creeping bentgrass seeds, and the mixing ratio of the common bermudagrass seeds to the creeping bentgrass seeds is 1:2.

[0011] Furthermore, in step S1, the disinfectant I is a 75% to 90% ethanol solution, and the disinfection time of the disinfectant I is 1 to 2 minutes; the disinfectant II is a sodium hypochlorite solution with an effective chlorine concentration of 0.05% to 0.06%, and the disinfection time of the disinfectant II is 15 to 25 minutes.

[0012] Furthermore, in step S2, the culture medium and the complexing solution are sterilized at high temperature before use, the sterilization temperature is 121° C., and the sterilization time is 15 to 20 minutes.

[0013] Furthermore, in step S2, the culture medium includes MS powder, 20-30 g / L sodium alginate solution and growth hormone, the added amount of the MS powder is 0.44-0.88 g; and the growth hormone is 5-10 mg / L GA3 hormone.

[0014] Furthermore, in step S2, the complexing liquid is a calcium chloride solution with a mass fraction of 4% to 5%, and the complexing time is 5 to 10 minutes.

[0015] Furthermore, the temperature of the frozen storage in step S3 is 0 to 4° C., and the time is 7 to 90 days.

[0016] Furthermore, in step S3, the artificial endosperm culture and planting temperature is 25-30°C, the relative humidity is 65%, and the PAR is 800 μmol / m 2 / s, the photoperiod was 16h, and the culture time was 30 days.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1) The present invention first performs a two-step disinfection treatment on the seeds that need to be mixed sown to remove harmful bacteria carried by the seeds, ensure the health of the artificial endosperm prepared subsequently, and improve its germination rate. At the same time, the present invention uses a hydrogel method to embed artificial seeds, and uses sodium alginate and calcium chloride solutions to complex the artificial seeds that need to be mixed sown, thereby increasing the storage time of the artificial seeds.

[0019] 2) The artificial seeds provided by the present invention optimize the ratio between different artificial seeds, so that the planted lawn grass seeds can be reasonably matched and have high flatness. At the same time, compared with directly sowing artificial seeds, the present invention shortens the germination time of seeds by adding specific doses and types of growth hormones, which can make the artificial seeds more valuable. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1This is a physical picture of the artificial endosperm prepared in Example 1 of the present invention;

[0021] Figure 2 The artificial endosperm prepared in Example 4 of the present invention is cultured and germinated (above) and the commercially available seeds are cultured and germinated after being frozen for 90 days (below). DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the present invention more clear, the technical solution of the present invention will be clearly and completely described below in conjunction with embodiments.

[0023] Example 1

[0024] This embodiment provides a method for efficient mixed sowing using artificial seeds, comprising the following steps:

[0025] S1 Seed material disinfection: Mix the seed materials to be mixed in proportion and soak them in disinfectant I. After soaking, wash the seeds and then soak them in disinfectant II. Finally, wash the seeds and absorb the moisture of the seeds;

[0026] Specifically, 2000 creeping bentgrass seeds 'PennA4' and 1000 common bermudagrass seeds 'common' were fully mixed, and then the mixed seeds were soaked in 75% ethanol (the solution was obtained by mixing anhydrous ethanol and water in a volume ratio of 75:25) for 2 minutes, and then washed twice with distilled water and drained. 100 mL of sodium hypochlorite solution with an effective chlorine concentration of 0.056% was added to the mixed seeds and soaked for 15 minutes, during which time it was properly shaken 2-3 times and then washed with sterile water, rinsed and placed on sterile filter paper to absorb water, and sterile seeds were obtained.

[0027] S2: preparing artificial endosperm: inoculating the sterile seed material obtained in S1 onto the culture medium, suspending and mixing, and dropping the suspension into the complexing agent for complexation;

[0028] Specifically, 30 g / L sodium alginate solution was sterilized at 121°C for 15 min and then cooled to room temperature, 0.44 g MS powder was added thereto and mixed, and then GA3 hormone was added to prepare a culture medium, and the concentration of GA3 hormone was ensured to be 5 mg / L. After the above culture medium was mixed, the sterile seeds obtained in S1 were added and mixed and suspended.

[0029] Then prepare a sterile 4% CaCl2 solution (dissolve 20g of CaCl2 in 480g of water and sterilize at 121℃ for 15min), drop the mixed suspension into the CaCl2 solution, wait for 10min after all the suspension is added, then take out and refrigerate for later use. Figure 1 shown.

[0030] S3 storage and culture: The artificial endosperm obtained in S2 is frozen and stored, and when used, the refrigerated artificial endosperm is placed in a culture matrix to cultivate germination.

[0031] Specifically, the artificial endosperm obtained in step S2 is evenly sown into a culture dish containing peat soil, and then placed in a culture room with a relative humidity of 65% and a PAR of 800 μmol / m 2 / s, average temperature 30℃ / 25℃ (day / night), photoperiod 16h, culture for 30 days. Meanwhile, the artificial endosperm obtained in S2 can be stored at 4℃ for 7 days.

[0032] Example 2

[0033] The difference between this embodiment and embodiment 1 is that in step S1, the seeds are immersed in the sodium hypochlorite solution for 25 minutes, and the other experimental steps and parameters are the same as those in embodiment 1.

[0034] Example 3

[0035] The difference between this embodiment and embodiment 1 is that the concentration of the sodium alginate solution in step S2 is 20 g / L, and the other experimental steps and parameters are the same as those in embodiment 1.

[0036] Example 4

[0037] The difference between this embodiment and embodiment 1 is that the artificial endosperm cultured in step S3 is the artificial endosperm prepared in step S2 and refrigerated for 90 days. The other experimental steps and parameters are the same as those in embodiment 1. The culture conditions are as follows: Figure 2 shown.

[0038] Example 5

[0039] The difference between this embodiment and embodiment 1 is that the concentration of GA3 in the culture medium in step S2 is 10 mg / L, and the other experimental steps and parameters are the same as those in embodiment 1.

[0040] Comparative Example 1

[0041] The difference between this comparative example and Example 1 is that in step S1, the time for soaking the seeds in the sodium hypochlorite solution is 0 min, and the other experimental steps and parameters are the same as those in Example 1.

[0042] Comparative Example 2

[0043] The difference between this comparative example and Example 1 is that in step S1, the seeds are immersed in the sodium hypochlorite solution for 30 minutes, and the other experimental steps and parameters are the same as those in Example 1.

[0044] Comparative Example 3

[0045] The difference between this comparative example and Example 1 is that the concentration of the sodium alginate solution in step S2 is 10 g / L, and the other experimental steps and parameters are the same as those in Example 1.

[0046] Comparative Example 4

[0047] The difference between this comparative example and Example 1 is that the concentration of the sodium alginate solution in step S2 is 40 g / L, and the other experimental steps and parameters are the same as those in Example 1.

[0048] Comparative Example 5

[0049] The difference between this comparative example and Example 1 is that the concentration of GA3 in the culture medium in step S2 is 1 mg / L, and the other experimental steps and parameters are the same as those in Example 1.

[0050] Comparative Example 6

[0051] The difference between this comparative example and Example 1 is that in step S2, GA3 is not added to the culture medium, and 6-BA is used as a growth hormone at a concentration of 5 mg / L. The other experimental steps and parameters are the same as those in Example 1.

[0052] Comparative Example 7

[0053] The difference between this comparative example and Example 1 is that the cultivation method is direct seeding. The specific steps are to fully mix 2000 creeping bentgrass seeds 'PennA4' and 1000 common bermudagrass seeds 'common' and directly sow them in a culture dish containing peat soil, and then place it in a culture room with a relative humidity of 65% and a PAR of 800 μmol / m 2 / s, average temperature 30℃ / 25℃ (day / night), and photoperiod 16h for 30 days.

[0054] Experimental Example 1: Effects of Sodium Hypochlorite Sterilization Steps and Time on Germination of Mixed Seeds

[0055] Experimental method: Take the sterile seeds obtained in step S1 of Example 1, Example 2 and Comparative Example 1-2, and sow them in a sterilized culture dish containing moist filter paper. After 10 days, the germination rate and contamination rate of the seeds are counted. The experimental results are shown in Table 1 below, where germination rate = number of seeds germinated / total number of seeds inoculated × 100%, and contamination rate = number of contaminated seeds / total number of seeds inoculated × 100%.

[0056] Table 1: Experimental results of the effects of sodium hypochlorite sterilization steps and time on the germination of mixed seeds

[0057]

[0058]

[0059] Analysis of experimental results: From the table above, it can be seen that the contamination rate of seeds that are not disinfected with sodium hypochlorite is 100%, which will seriously affect the development and cultivation of seeds in the later cultivation process. If the disinfection time is too long, the germination rate of seeds will decrease rapidly. The disinfection time of 15 to 25 minutes can not only ensure that the mixed seeds are free from contamination, but also do not affect the germination rate of seeds.

[0060] Experimental Example 2: Effects of different sodium alginate concentrations on seeds

[0061] Experimental method: The artificial endosperms prepared in step S2 of Example 1, Example 3 and Comparative Examples 3-4 were placed in the culture environment of Example 1 to test the germination rate of seeds and the integrity of their seed coats. The experimental results are shown in Table 2 below:

[0062] Table 2: Experimental results of the effect of different sodium alginate concentrations on grass seed germination rate

[0063]

[0064] Analysis of experimental results: From the table above, it can be seen that when the sodium alginate concentration is 20-30g / L, the artificial endosperm prepared can maintain a high germination rate and a high integrity of the seed coat surface. When the concentration of sodium alginate is reduced, the artificial endosperm cannot be formed, and when the concentration of sodium alginate is too high, the seeds cannot maintain a normal germination rate.

[0065] Experimental Example 3: Effects of different growth hormone concentrations and types on seed germination time

[0066] Experimental method: The artificial endosperms prepared in step S2 of Example 1, Example 5 and Comparative Examples 5 and 6 were cultured according to the culture conditions to test the germination time of the seeds. The experimental results are shown in Table 3 below:

[0067] Table 3: Experimental results of the effects of different growth hormone concentrations and types on the germination time of grass seeds

[0068]

[0069] Analysis of experimental results: From the above experimental results, it can be seen that when 5 mg / L and 10 mg / L GA3 are used as growth hormone in Examples 1 and 5 of the present invention, the average germination time of the artificial endosperm is about 3 days. However, when the concentration of growth hormone is reduced or 6-BA is used instead of GA3, the germination time of the artificial seeds increases to about 5 days.

[0070] Comparing the test results of the embodiment and comparative example 7, the germination rate of the artificial seeds sown by direct mixed sowing is about 99%, but the average germination time is about 5 days, which is 2 days longer than the germination time of the artificial endosperm in the embodiment of the present invention. At the same time, the lawn grass seeds cultivated by the artificial seeds sown by direct mixed sowing are unevenly mixed, and the lawn quality is poor.

[0071] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view. In addition, it should be understood that although this specification is described in accordance with the embodiments, it does not contain only one technical solution. This narrative of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in the embodiments can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A method for efficient mixed sowing using artificial seeds, characterized in that: The following steps are involved: S1 seed material disinfection: the seed materials to be mixed and sown are mixed in proportion and then soaked in disinfectant I, washed after soaking and then soaked in disinfectant II, finally washed and dried, the seed materials include common bermudagrass seeds and creeping bentgrass seeds, and the mixing ratio of the common bermudagrass seeds to the creeping bentgrass seeds is 1:2; S2: preparing artificial endosperm: inoculating the sterile seed material obtained in S1 onto the culture medium for suspension and mixing, and dropping the suspension into the complexing agent for complexing. The culture medium includes MS powder, 20-30 g / L sodium alginate solution and growth hormone, wherein the amount of MS powder added is 0.44-0.88 g; the growth hormone is 5-10 mg / L GA3 hormone, the complexing liquid is 4%-5% calcium chloride solution by mass, and the complexing time is 5-10 min; S3 storage and culture: The artificial endosperm obtained in S2 is frozen and stored, and when used, the refrigerated artificial endosperm is placed in a culture matrix to cultivate germination.

2. The method for efficient mixed sowing using artificial seeds according to claim 1, characterized in that: Place In step S1, the disinfectant I is a 75% to 90% ethanol solution, and the disinfection time of the disinfectant I is 1 to 2 minutes; the disinfectant II is a sodium hypochlorite solution with an effective chlorine concentration of 5.5% to 6%, and the disinfection time of the disinfectant II is 15 to 25 minutes.

3. The method for efficient mixed sowing using artificial seeds according to claim 1, characterized in that: Place In step S2, the culture medium and the complexing solution are sterilized at high temperature before use, the sterilization temperature is 121° C., and the sterilization time is 15 to 20 minutes.

4. The method for efficient mixed sowing using artificial seeds according to claim 3, characterized in that: In step S2, the complexing liquid is a calcium chloride solution with a mass fraction of 4% to 5%, and the complexing time is 5 to 10 minutes.

5. A method for efficient mixed sowing using artificial seeds according to claim 1, characterized in that: The temperature of the frozen storage in step S3 is 0 to 4° C., and the time is 7 to 90 days.

6. A method for efficient mixed sowing using artificial seeds according to claim 5, characterized in that: In step S3, the artificial endosperm culture and planting temperature is 25-30° C., the relative humidity is 65%, and the PAR is 800 μmol / m 2 / s, the photoperiod was 16h, and the culture time was 30 days.

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