A pesticide composition and its application

Abstract

The present invention belongs to the technical field of pesticide acaricides and discloses a pesticide composition and its use. The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide, and active ingredient B is either spirodiclofen or bifenazate. The mass ratio of active ingredient A to active ingredient B is 1:52 to 45:1. The pesticide composition of the present invention has a good control effect on citrus spider mites and two-spotted spider mites, and has excellent fast-acting properties and a long-lasting effect.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide acaricide and discloses a pesticide composition and application thereof. Background Art

[0002] (4'-Chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide is a biphenyl sulfide acaricide with excellent control effects on common plant pests. Its structural formula is shown below:

[0003]

[0004] Spirodiclofen is a highly effective, low-toxic acaricide developed by Bayer. It primarily inhibits fat synthesis in the mite's body, blocking energy metabolism. It is effective at all stages of mite development, especially against mite eggs and nymphs, and has a certain inhibitory effect on the reproduction of adult female mites. Its structural formula is as follows:

[0005]

[0006] Bifenazate, chemically known as isopropyl 3-(4-methoxybiphenyl-3-yl)hydrazinecarboxylate, is a miticide that primarily acts at the Qo site of cytochrome b complex III in the electron transport chain. It is effective against all developmental stages of spider mites. Its structural formula is as follows:

[0007]

[0008] Due to the rough field production methods, agricultural growers use a large amount of insecticides and acaricides, which has caused the field mite population to develop varying degrees of resistance to common acaricides. Therefore, the development of acaricides that have obvious synergistic effects on pest mites and can reduce the dosage of pesticides is an important trend in current agricultural development. Summary of the Invention

[0009] Based on the problems in the prior art, the present invention provides a pesticide composition, which has excellent control effects on harmful mites, especially significant synergistic effects on common two-spotted spider mites and citrus mites, reduces the dosage of the agent, has excellent fast-acting properties and a long lasting effect.

[0010] The present invention also provides various formulations of the pesticide composition, which are used for preventing and controlling Tetranychus urticae and Panonychus citri.

[0011] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition, comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide, and the active ingredient B is either spirodiclofen or bifenazate;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:52 to 45:1;

[0013] Furthermore, the active ingredient B is spirodiclofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:52 to 44:1;

[0014] The active ingredient B is bifenazate, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 40:1.

[0015] Furthermore, the active ingredient B is spirodiclofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:33 to 38:1;

[0016] The active ingredient B is bifenazate, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 40:1.

[0017] Furthermore, the total weight of the pesticide composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.1 wt% to 80 wt% of the total weight of the pesticide composition;

[0018] Furthermore, the pesticide composition further comprises auxiliary ingredients in addition to the active ingredient, and the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0019] Furthermore, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0020] The dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0021] The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose; and / or

[0022] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0023] The emulsifier is selected from one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester; and / or

[0024] The defoaming agent is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8~C 10 One or more of fatty alcohol compounds or silicone compounds; and / or

[0025] The preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate; and / or

[0026] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, epoxidized vegetable oil, and / or

[0027] The synergist is selected from synergist phosphine and piperonyl butoxide; and / or

[0028] The carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and white carbon black; and / or

[0029] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water; and / or

[0030] All of the above auxiliary ingredients are commercially available.

[0031] Furthermore, the pesticide composition can be prepared into a formulation in the form of a solid preparation or a liquid preparation;

[0032] Furthermore, the liquid preparation is selected from soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions; the solid preparation is selected from powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0033] Furthermore, the solid preparation is a water-dispersible granule or a wetting dispersant, and the liquid preparation is a suspension, an aqueous emulsion, a microemulsion, an emulsifiable concentrate, a dispersible oil suspension, or a suspoemulsion.

[0034] The present invention also discloses the use of the above pesticide composition and its preparation for preventing and controlling harmful mites.

[0035] Furthermore, the pest mite is a Tetranychus pest mite; the Tetranychus pest mite is Panonychus citri, Panonychus apical, Tetranychus urticae or Tetranychus cinnabarinus;

[0036] Furthermore, the harmful mites of the Tetranychus family are Panonychus citri and Tetranychus urticae.

[0037] The beneficial effects of the present invention are as follows:

[0038] 1. The pesticide composition of the present invention has a significant synergistic effect on common plant pests, especially on spider mites and citrus mites, and can effectively reduce the number of pesticide applications and the amount of a single dose used;

[0039] 2. The pesticide composition of the present invention is composed of active ingredients with different mechanisms of action, which can effectively slow down the development of drug resistance in harmful mites. DETAILED DESCRIPTION

[0040] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described using the following specific implementation methods. However, the present invention can be implemented in various forms and should not be limited by the implementation methods described herein.

[0041] Some examples of preparation of related preparations:

[0042]

[0043] Indoor toxicity test

[0044] Example 1: Indoor active ratio screening test

[0045] Test targets: nymphs of Tetranychus urticae and Panonychus citri.

[0046] The test was conducted with reference to NY / T 1154.13-2008 “Guidelines for Indoor Bioassay Tests of Pesticides—Insecticides Part 13: Leaf Disk Spray Method”.

[0047] Test Method: Leaves from beans and citrus trees, grown and untreated, were used. Leaf discs were punched out of the leaves and placed, dorsal surface upward, in moisturizing petri dishes. Two leaf discs were placed in each dish. Nymphs were then transferred to the discs, with 15 mites per disc. Once the mites were stable, sprays were applied using a potter spray tower, starting with low and then increasing concentrations. Each treatment was repeated four times, with 1.5 mL per treatment and allowed to settle for 1 minute. A control area was treated with clean water.

[0048] After treatment, the test mites were transferred to a temperature of (25±1)°C and a photoperiod of L:D = (16:8) h for rearing and observation. Each treatment was replicated four times, and a treatment without the agent (containing all organic solvents and emulsifiers) served as a blank control. Nymph mortality was examined 48 hours after treatment, and the total number of mites and the number of dead mites were recorded.

[0049] According to statistics and analysis:

[0050] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0051]

[0052] Where:

[0053] P——mortality rate, in percentage (%);

[0054] K——indicates the number of dead insects, the unit is head;

[0055] N——represents the total number of insects processed, in heads.

[0056]

[0057] Where:

[0058] P1——adjusted mortality rate, in percentage (%);

[0059] P t ——Treatment mortality rate, expressed in percentage (%);

[0060] P0 - blank control mortality rate, in percentage (%).

[0061] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0062] The obtained test data were analyzed using the DPS statistical analysis system to obtain the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0063] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0064]

[0065] Where:

[0066] ATI - measured toxicity index of mixture;

[0067] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0068] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0069] TTI=TI A ×P A +TI B ×P B

[0070] Where:

[0071] TTI – Theoretical Toxicity Index of Mixtures;

[0072] TI A ——Agent toxicity index;

[0073] P A ——The percentage of agent A in the mixture, in percentage (%);

[0074] TI B ——Toxicity index of agent B;

[0075] P B——The percentage of agent B in the mixture, in percentage (%).

[0076]

[0077] Where:

[0078] CTC – Co-toxicity coefficient;

[0079] ATI - measured toxicity index of mixture;

[0080] TTI - Theoretical Toxicity Index of Mixture.

[0081] A co-toxicity coefficient (CTC) of ≥120 indicates a synergistic effect; a CTC of ≤80 indicates an antagonistic effect; and a CTC of 80 < CTC <120 indicates an additive effect. The results of the indoor activity test are shown in the table below:

[0082] Table 1 Indoor biological activity test of active ingredient A combined with spiroclofen against nymphs of Tetranychus urticae

[0083]

[0084]

[0085] Table 1 shows that the combination of active ingredient A and spirodiclofen exhibited a significant synergistic effect against Tetranychus urticae within a reasonable range. The mass ratio of active ingredient A to spirodiclofen ranged from 1:52 to 44:1, with a co-toxicity coefficient greater than 120, indicating a synergistic effect.

[0086] Table 2 Indoor biological activity test of active ingredient A combined with bifenazate against nymphs of Tetranychus urticae

[0087]

[0088] The results of the indoor activity test are shown in Table 2. When the active ingredient A is combined with bifenazate at a mass ratio of 1:30 to 40:1, the co-toxicity coefficient is greater than 120, showing a synergistic effect against two-spotted spider mites.

[0089] Table 3 Indoor bioactivity test of active ingredient A combined with spiroclofen on citrus nymphs

[0090]

[0091] The results of the indoor activity test are shown in Table 3. The mass ratio of active ingredient A to spirodiclofen was 1:33 to 38:1, and the co-toxicity coefficient was greater than 120. The combined effect on Panonychus citri was synergistic.

[0092] Table 4 Indoor bioactivity test of active ingredient A combined with bifenazate against citrus nymphs

[0093]

[0094] The results of the indoor activity test are shown in Table 4. The mass ratio of active ingredient A to bifenazate is 1:35-40:1, the co-toxicity coefficient is greater than 120, and the combined effect on citrus mites is synergistic.

[0095] Field efficacy verification test

[0096] Example 2: Field control test of Tetranychus urticae

[0097] This test was conducted with reference to GB / T 17980.17-2000 “Guidelines for field efficacy tests of pesticides (I) Acaricides for the control of spider mites on beans and vegetables”.

[0098] The experiment was carried out in Zhangqiao Village, Shouguang City, Shandong Province. The soil fertility of the experimental site was above average.

[0099] Experimental crop: loofah.

[0100] Test target: Two-spotted spider mite.

[0101] Experimental design: The experimental plots, control plots and blank control plots were arranged in random blocks. The cultivation conditions of all experimental plots were uniform and consistent, in line with local scientific agricultural practices. The experimental plot area was 30m 2 , each treatment was repeated 4 times.

[0102] Application time: Apply the pesticide once on September 20, 2022, with a water consumption of 45 kg per mu. Spray the pesticide once when the mite control index reaches about 10% of the leaves, with 3 to 10 adult mites on each leaf.

[0103] Test survey time: Take 20 leaves from each plot to count the number of live mites and nymphs. Conduct a base survey before the test and conduct surveys 3, 7, and 14 days after the test.

[0104] Calculation method of drug efficacy:

[0105]

[0106] Table 5 Field control test results of spider mites in loofah

[0107]

[0108] The results of the field efficacy test for controlling spider mites showed (Table 5) that the pesticide composition of the present invention has a good control effect on spider mites of the sponge gourd, and has good fast-acting property and a long lasting effect.

[0109] Example 3: Field test for controlling citrus spider mites

[0110] Refer to GB / T 17980.11-2000 "Guidelines for field efficacy tests of pesticides (I) Acaricides for the control of Panonychus citri".

[0111] Test location: This test was conducted in Shuangqiao Town, Wuming District, Nanning City, Guangxi Zhuang Autonomous Region. The trees in each plot of the test site were of the same age and had basically the same crown size.

[0112] Experimental crops: citrus.

[0113] Test target: Citrus spider mite.

[0114] Test agent: The test plot area consists of 2 fruit trees, and each treatment is replicated 4 times. The plots for the test agent, control agent, and blank control are arranged in randomized blocks.

[0115] Application time: Apply pesticide once on October 28, 2022, apply 1.5L of pesticide per plant, use an electric sprayer to apply pesticide to the whole citrus tree, and apply the pesticide until the leaves are wet but not dripping.

[0116] Survey Method: Mark the young shoots of each tree in each plot at five locations: east, west, south, north, and center. Count the number of active mites on 20 leaves. Observe the leaf surface directly using a handheld magnifying glass to count the number of mites.

[0117] Survey time and frequency: Survey once every 3 days and 15 days after treatment, and record the number of active mites.

[0118] Calculation method of drug efficacy:

[0119]

[0120] The results of the field efficacy test are shown in the table below:

[0121] Table 6 Results of field trials on citrus spider mites

[0122]

[0123]

[0124] The results of the field efficacy control test (Table 6) show that the pesticide composition of the present invention has a good control effect on citrus red spider mites. Three days after application, the control efficacy of Preparation Example 2 and Preparation Example 5 on citrus spider mites is higher than 90%.

[0125] During the above field trials, no phytotoxicity was found on the target crops, nor was any adverse effect on non-target organisms.

[0126] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that The pesticide composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide, and the active ingredient B is spirodiclofen. The mass ratio of the active ingredient A to the active ingredient B is 1:52 to 44:

1.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:33-38:

1.

3. The pesticide composition according to claim 1, characterized in that The total weight of the pesticide composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.1 wt% to 80 wt% of the total weight of the pesticide composition.

4. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also includes auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The pesticide composition according to claim 1, characterized in that The pesticide composition can be prepared into a solid preparation or a liquid preparation.

6. The pesticide composition according to claim 5, characterized in that The solid preparation is a water-dispersible granule or a wetting dispersant, and the liquid preparation is a suspension, an aqueous emulsion, a microemulsion, an emulsifiable concentrate, a dispersible oil suspension or a suspoemulsion.

7. Use of the pesticide composition according to any one of claims 1 to 6 for controlling pest mites, characterized in that: The harmful mite is Panonychus citri or Tetranychus urticae.

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