An apparatus for culturing cells under adjustable fluid shear stress

Abstract

The present invention discloses a device for cell culture under adjustable fluid shear force, which relates to cell culture. It includes a protective housing, an installation component is installed inside the protective housing, a central connecting piece is installed in the middle of the installation component, the installation component includes an installation plate, a plurality of annularly distributed grooves are formed on the installation plate, each group of grooves includes a plurality of installation grooves, and a guide rail component is fixedly installed on the lower surface of the installation plate. By setting the installation component, the installation component generates a wavy swing during use, and the culture solution inside it will be driven to flow in a circulating manner, thereby creating shear stress. There is no need to use a pump body, and the swinging method can not only achieve the flow of liquid, but also create impact and vibration effects, which is more in line with the real environment in the organism, and the cost is relatively low. Because the pump body is removed, the overall volume of the device is small and convenient to move, so that it can be applied to more experiments.

Description

Technical Field

[0001] The present invention relates to the field of cell culture, and particularly to a device for culturing cells under adjustable fluid shear force. Background Art

[0002] In vivo, fluid flow generates shear stress, which is a mechanical force that affects cell morphology and behavior in various ways. In many standard in vitro experiments, cells are cultured under static conditions without flow. Under these static conditions, cell changes related to shear stress cannot be considered. In contrast, in vitro cell culture under flow conditions mimics this mechanical stimulation and induces a more physiological, in vivo-like behavior. When using cells in a biological fluid system (such as endothelial cells or epithelial cells), it is particularly important to work under flow conditions.

[0003] Existing cell culture devices under fluid shear force on the market mimic the shear stress in vivo by forming liquid flushing in a pipeline. For example: the ibidi pump system / fluid shear force system. The ibidi fluid shear force system includes two main parts: the ibidi pump (computer-controlled) that generates air pressure and a portable fluid unit (two culture medium reservoirs, a μ-Slide fixing device, and a flow pipeline). The ibidi fluid shear force system controls parameters such as flow rate and shear force magnitude through the pump, and realizes the self-circulation of cell culture medium in the two reservoirs through the opening and closing states of the two valves in the fluid unit. Furthermore, only a very small amount of cell culture medium is required for long-term cell culture, and the small amount of culture medium is also convenient for analyzing changes in soluble factors produced by cells. Unidirectional flow of cell culture medium can be achieved through a single fluid unit, and pulsed flow or oscillatory flow of cell culture medium can be achieved by combining two fluid units. The portable fluid unit can be easily connected to and disconnected from the pump, making it simple to operate the fluid unit in a laminar flow hood, such as adding culture medium to the reservoir and connecting a slide for culturing cells. After preparation, the fluid unit is placed in a cell culture incubator and connected to the pump for gas path and electrical connection, and then cell culture under fluid shear force can be carried out.

[0004] Existing cell culture devices under fluid shear force mimic the shear stress in vivo by forming liquid flushing in a pipeline. For example: the ibidi pump system / fluid shear force system. However, the price of this kind of device is generally high, the volume is relatively large, and it is relatively inconvenient to use. Therefore, we have developed a practical and convenient device for cell culture under fluid shear force. Summary of the Invention

[0005] The purpose of the present invention is to provide a device for culturing cells under adjustable fluid shear force to solve the problems raised in the above background art.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A device for cell culture under adjustable fluid shear force, comprising a protective housing, an installation component is installed inside the protective housing, a central connector is installed in the middle of the installation component, and the installation component includes an installation plate;

[0008] A plurality of groups of annularly distributed grooves are provided on the installation plate, each group of grooves includes a plurality of installation grooves, a culture bottle is placed inside the installation groove, a guide rail component is fixedly installed on the lower surface of the installation plate, the central connector includes a spherical central member, a lower support rod is fixedly installed below the spherical central member, a rotating plate is rotatably installed in the middle section of the lower support rod, a telescopic member is fixedly installed on one side of the rotating plate, a collar is fixedly installed at the end of the telescopic member away from the rotating plate, a hydraulic cylinder is fixedly installed on the lower surface of the collar, the telescopic end of the hydraulic cylinder has a piston rod, the piston rod is slidably clamped in the middle of the collar, a slider is slidably clamped inside the guide rail component, and the top end of the piston rod is connected to the middle of the lower surface of the slider through ball socket fit.

[0009] Optionally, the culture bottle includes an annular flat bottle for culturing cells, bottle mouths for putting cells are fixedly installed on both sides of the upper surface of the annular flat bottle, a bottle stopper is detachably clamped inside the bottle mouth, the annular flat bottle and the bottle mouths are made of biological glass material, the bottle stopper is made of ordinary glass material, and the connection positions between the bottle stopper and the bottle mouths are all treated with ground glass.

[0010] Optionally, a plurality of uniformly distributed clamping grooves are provided at the outer circle of the installation groove, two convex blocks are fixedly installed at the positions corresponding to the clamping grooves on the upper surface of the installation plate, a first L-shaped blocking rod is rotatably installed between the two convex blocks, a sliding groove is provided below each of the plurality of clamping grooves, a second L-shaped blocking rod is slidably clamped inside the sliding groove, one end of the second L-shaped blocking rod has an inclined protrusion, a plurality of uniformly distributed pressing springs are fixedly installed between the second L-shaped blocking rod and the sliding groove, pressing plates are provided above both sides of the installation groove, and the ends of the second L-shaped blocking rods away from the inclined protrusions all extend to the outside of the installation plate 21 and are fixedly installed on the lower surfaces of the corresponding pressing plates.

[0011] Optionally, a ball socket type kit is fixedly installed in the middle of the installation plate, and the ball socket type kit is sleeved on the outer surface of the spherical central member through ball socket fit.

[0012] Optionally, an upper connecting rod is fixedly installed above the spherical central member, a bearing box is fixedly installed at the end of the upper connecting rod away from the spherical central member, and a culture bottle is also placed inside the bearing box.

[0013] Optionally, uniformly distributed fixing rods are fixedly installed at the outer ring of the carrying box, and one ends of the fixing rods away from the carrying box are fixedly installed at the inner ring of the protection housing.

[0014] Optionally, a plurality of annularly distributed spring telescopic rods are fixedly installed on the lower surface inside the protection housing, a connecting plate is installed at the telescopic end of the spring telescopic rods, and the connecting plate is slidably installed on the lower surface of the placement plate.

[0015] Optionally, a torsion spring is fixedly installed between the bump and the first L-shaped stop rod.

[0016] Optionally, a sealing cover plate is installed at the opening of the protection housing, and a support base is fixedly installed at the bottom end of the protection housing.

[0017] Optionally, a motor is fixedly installed on the support base, and the driving end of the motor penetrates through the protection housing and the lower support rod and is fixedly installed in the middle of the rotating plate.

[0018] The technical solution provided by the present invention has at least the following beneficial effects compared with the prior art:

[0019] In the above solution, by setting the placement component, when in use, the placement component generates a wavy swing, so that the culture bottles on its surface will also swing accordingly. When the placement component generates a wavy undulating swing, the culture solution inside the culture bottle will flow under the action of the swing. Because the shape of the culture bottle is annular, when the culture bottle swings, the culture solution inside it will be driven to circulate, thereby creating a shear stress. There is no need to use a pump body, and the use of the swing method can not only achieve the flow of the liquid, but also create impact and vibration effects, which is more in line with the real environment in the organism, and the cost is low. Because the pump body is removed, the overall volume of the device is small and convenient to move, so that it can be applied to more experiments;

[0020] By setting the carrying box, the culture bottles inside the carrying box can be used as a control group experiment, so that they can be in the same environment as the culture bottles on the placement component, only the state of the liquid flow changes, ensuring that other factors are the same, ensuring the accuracy of the control experiment, and preventing the external environment from affecting the control group;

[0021] By setting multiple groups of placement grooves, different flow velocities can be generated inside one device, thereby generating different shear stresses, which is convenient for studying the influence of the magnitude of the shear stress on cell culture experiments, so that only the shear stress is different and other factors are the same, such as the influence of temperature and humidity factors, ensuring the accuracy of the experiment, and multiple groups of experiments can be carried out simultaneously, which is convenient for intuitively understanding the influence of shear force on cell culture;

[0022] By setting up a hydraulic cylinder, during operation, the hydraulic cylinder is controlled to contract quantitatively, and then the motor is controlled to operate, realizing the swing of the placement component, thereby driving the culture solution to flow and generating shear stress. Controlling the rotation speed of the motor and the contraction amount of the hydraulic cylinder can indirectly change the magnitude of the shear stress. The operation is simple and easy to start without much experience. Even students in their first experiments can easily get started;

[0023] By setting up a culture bottle, using bio-glass can play a certain role in promoting cell culture. Since the annular flat bottle is circular, under the action of the placement component, the culture solution is easier to flow inside it. And the annular flat bottle is flat. After the culture is completed, the culture bottle can be directly placed under the microscope, and then a staining solution, etc. can be added to it without using a glass slide for sampling and then observing. Setting two bottle mouths facilitates adding cells, replacing the culture medium, and digesting cells, making the experiment more convenient;

[0024] By setting up the first L-shaped stop rod, during installation, only by pressing the culture bottle into the inside of the placement groove can automatic clamping be achieved. When the culture bottle needs to be taken out, only by pressing the pressing plate can the first L-shaped stop rod be released, thereby losing the effect on the culture bottle. At this time, only by pulling the culture bottle out of the placement groove can it be taken out, which is convenient for installation and removal. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The drawings incorporated herein and constituting a part of the specification illustrate embodiments of the present invention and, together with the specification, are further used to explain the principles of the present invention and enable those skilled in the relevant art to implement and use the present invention.

[0026] Figure 1 It is a schematic structural diagram of the device for culturing cells under adjustable fluid shear stress of the present invention.

[0027] Figure 2 It is a schematic structural diagram of the interior of the protective housing of the present invention.

[0028] Figure 3 It is a schematic connection structure diagram of the guide rail component and the spring telescopic rod of the present invention.

[0029] Figure 4 It is a schematic structural diagram of the central connecting piece of the present invention.

[0030] Figure 5 It is a schematic structural diagram of the placement component of the present invention.

[0031] Figure 6 It is a schematic structural diagram of the culture bottle of the present invention.

[0032] Figure 7 It is a partial schematic structural diagram of the placement plate of the present invention.

[0033] Figure 8 This is a partial sectional view of the placement plate in the present invention.

[0034] [Reference numerals]

[0035] In the figure:

[0036] 1. Protection housing; 11. Sealing cover plate; 12. Support base;

[0037] 2. Placement component; 21. Placement plate; 211. Protrusion; 212. Chute; 22. Placement groove; 221. Card slot; 23. First L-shaped stop bar; 24. Second L-shaped stop bar; 241. Inclined protrusion; 25. Pressing plate; 26. Pressing spring; 27. Ball socket kit;

[0038] 3. Central connecting piece; 31. Spherical central piece; 32. Lower support rod; 33. Upper connecting rod; 34. Carrying box; 341. Fixed rod; 35. Rotating plate; 36. Telescopic member; 37. Collar; 38. Hydraulic cylinder; 381. Piston rod; 39. Slide block;

[0039] 4. Guide rail component;

[0040] 5. Spring telescopic rod; 51. Connecting plate;

[0041] 6. Culture bottle; 61. Annular flat bottle; 62. Bottle mouth; 63. Bottle stopper.

[0042] As shown in the figure, in order to clearly show the structure of the embodiments of the present invention, specific structures and devices are marked in the figure, but this is only for illustrative purposes and is not intended to limit the present invention to this specific structure, device and environment. Those of ordinary skill in the art can adjust or modify these devices and environments according to specific needs. Detailed implementation manners

[0043] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0044] Such as Figures 1 to 8As shown, an embodiment of the present invention provides a device for culturing cells under adjustable fluid shear force, including a protective housing 1. A sealing cover plate 11 is installed at the opening of the protective housing 1. An installation component 2 is installed inside the protective housing 1. A central connecting component 3 is installed in the middle of the installation component 2. The central connecting component 3 includes a spherical central part 31. An upper connecting rod 33 is fixedly installed above the spherical central part 31. One end of the upper connecting rod 33 away from the spherical central part 31 is fixedly installed with a bearing box 34. Uniformly distributed fixing rods 341 are fixedly installed at the outer circle of the bearing box 34. One end of each fixing rod 341 away from the bearing box 34 is fixedly installed at the inner circle of the protective housing 1. The installation component 2 includes an installation plate 21. Multiple groups of annularly distributed grooves are formed on the installation plate 21. Each group of grooves includes multiple installation grooves 22. A culture bottle 6 is placed inside each installation groove 22. A culture bottle 6 is also placed inside the bearing box 34. The culture bottle 6 includes an annular flat bottle 61 for culturing cells.

[0045] During actual use, first inject the cells to be cultured and the culture medium into the inside of the culture bottle 6, then place the culture bottle 6 into the corresponding installation groove 22 and the bearing box 34, and then start the device. The installation component 2 can swing in a wavy manner around the central axis, so that the culture medium inside the culture bottle 6 flows, and then an impact is generated to form fluid shear force. Because when cells are in the body, the body fluid is constantly flowing, and shear force will be generated during the flowing process. However, in many existing cell cultures, this characteristic is ignored, and a static culture environment is used to cultivate cells. This method cannot consider the cell changes related to shear stress and cannot truly simulate the mechanical stimulation generated by the flowing of body fluid in the human body. A flowing environment can induce a more physiological and in-vivo-like behavior. When using cells in a biofluid system (such as endothelial cells or epithelial cells), it is particularly important to work under flowing conditions.

[0046] In the prior art, in order to simulate such a flowing environment, most of them form liquid scouring in a pipeline to imitate the shear stress in the body, for example: ibidi pump system / fluid shear force system. However, the price of this kind of device is generally high, the volume is relatively large, and it is not very convenient to use.

[0047] By setting the placement component 2, the placement component 2 generates a wavy swing during use, causing the culture flask 6 on its surface to also swing accordingly. When the placement component 2 generates a wavy undulating swing, the culture solution inside the culture flask 6 will flow under the action of the swing. Since the shape of the culture flask 6 is annular, when the culture flask 6 swings, the culture solution inside it will be driven to flow in a cyclic manner, thereby creating a shear stress. There is no need to use a pump body, and the use of the swing method can not only achieve the flow of the liquid, but also create impact and vibration effects, which is more in line with the real environment in the organism, and the cost is relatively low, making it applicable to more experiments.

[0048] During use, the culture flask 6 on the placement component 2 will swing with the swing of the placement component 2. However, since the central connecting piece 3 is fixedly installed inside the protective housing 1, the culture flask 6 inside the carrier box 34 will not move. The culture flask 6 inside the carrier box 34 is in a relatively stable environment. The culture flask 6 inside the carrier box 34 can be used as a control group experiment, enabling it to be in the same environment as the culture flask 6 on the placement component 2, only the state of liquid flow changes, ensuring that all other factors are the same, guaranteeing the accuracy of the control experiment, and preventing the external environment from affecting the control group.

[0049] During use, first place the culture flask 6 inside the placement groove 22. When the culture flask 6 is placed in the placement grooves 22 at different positions, the forces it receives are different. When the culture flask 6 is located near the spherical center piece 31, the centrifugal force it receives is small, and thus the flow force generated by the liquid is also small, that is, the shear stress is small. While the culture flask 6 on the side far from the spherical center piece 31 has a larger centrifugal force because the distance increases, and thus the flow rate of the liquid inside the culture flask 6 is faster, and the generated shear stress is higher. This enables different flow rates to be generated inside one device, and thus different shear stresses, facilitating the study of the influence of the magnitude of shear stress on cell culture experiments, making only the shear stress different while all other factors are the same, such as the influence of temperature and humidity factors, guaranteeing the accuracy of the experiment, and enabling multiple groups of experiments to be carried out simultaneously, facilitating an intuitive understanding of the influence of shear force on cell culture.

[0050] The lower surface of the placement plate 21 is fixedly installed with a guide rail component 4. The middle part of the placement plate 21 is fixedly installed with a ball socket type kit 27. The ball socket type kit 27 is sleeved on the outer surface of the spherical center piece 31 through ball socket fitting. A lower support rod 32 is fixedly installed below the spherical center piece 31. A rotating plate 35 is rotatably installed in the middle section of the lower support rod 32. One side of the rotating plate 35 is fixedly installed with a telescopic rod member 36. The end of the telescopic rod member 36 away from the rotating plate 35 is fixedly installed with a collar 37. The lower surface of the collar 37 is fixedly installed with a hydraulic cylinder 38. The telescopic end of the hydraulic cylinder 38 has a piston rod 381. The piston rod 381 is slidably clamped in the middle of the collar 37. A slider 39 is slidably clamped inside the guide rail component 4. The top end of the piston rod 381 is connected to the middle of the lower surface of the slider 39 through ball socket fitting. The bottom end of the protection shell 1 is fixedly installed with a support base 12. A motor is fixedly installed on the support base 12. The driving end of the motor penetrates through the protection shell 1 and the lower support rod 32 and is fixedly installed in the middle of the rotating plate 35. A plurality of spring telescopic rods 5 distributed in a ring are fixedly installed on the inner lower surface of the protection shell 1. The telescopic end of the spring telescopic rod 5 is installed with a connecting plate 51. The connecting plate 51 is slidably installed on the lower surface of the placement plate 21. The spring telescopic rod 5 can not only restore the placement component 2 to a horizontal state, but also increase the stability and continuity when the placement component 2 swings.

[0051] Before use, the hydraulic cylinder 38 extends to the maximum state. Under the action of a plurality of spring telescopic rods 5, the placement plate 21 maintains a horizontal state. The connecting plate 51 can increase the connection length and prevent the placement component 2 from separating from the spring telescopic rod 5 when swinging. After the culture bottle 6 is installed, control the hydraulic cylinder 38 to contract the piston rod 381. At this time, the piston rod 381 drives the slider 39 to move downward, and then the placement plate 21 is driven to be inclined. The inclination angle of the placement plate 21 can be changed by controlling the contraction amount of the hydraulic cylinder 38. At this time, start the motor. The motor drives the rotating plate 35 to rotate. The rotating plate 35 drives the telescopic rod member 36 to rotate. Then the telescopic rod member 36 drives the collar 37, the hydraulic cylinder 38, and the piston rod 381 to rotate. Then the piston rod 381 will drive the slider 39 to slide in a circular shape along the inside of the guide rail component 4. Because the connecting plate 51 is slidably installed between the placement plate 21, the placement plate 21 will not rotate, but will be driven to swing in a wavy shape around the spherical center piece 31. The force on the culture bottle 6 above it can be changed by changing the rotation speed of the motor and the contraction amount of the hydraulic cylinder 38. Then the flow rate and shear stress of the culture solution inside the culture bottle 6 can be controlled. By setting the hydraulic cylinder 38, control the hydraulic cylinder 38 to contract quantitatively during work, and then control the motor to work to realize the swing of the placement component 2, and then drive the culture solution to flow to generate shear stress. Controlling the rotation speed of the motor and the contraction amount of the hydraulic cylinder 38 can indirectly change the magnitude of the shear stress. The operation is simple and easy to start without much experience. Even students who are doing experiments for the first time can easily get started.

[0052] On both sides of the upper surface of the annular flat bottle 61, there are fixedly installed bottle mouths 62 for placing cells. A bottle stopper 63 is detachably clamped inside the bottle mouth 62. The annular flat bottle 61 and the bottle mouth 62 are both made of bioglass material, the bottle stopper 63 is made of ordinary glass material, and the connection positions between the bottle stopper 63 and the bottle mouth 62 are all treated with frosted glass.

[0053] Bioglass is a kind of glass material with specific biological and physiological functions. The degradation products of bioglass can promote the generation of growth factors, promote the reproduction of cells, enhance the gene expression of osteoblasts and the growth of bone tissue. Using bioglass can play a certain role in promoting cell culture. Because the annular flat bottle 61 is circular, under the action of the placement component 2, the culture solution is more likely to flow inside it. And the annular flat bottle 61 is flat. After the culture is completed, the culture bottle 6 can be directly placed under the microscope, and then a heat solution, etc. can be added to it, without the need to use a glass slide for sampling and then observing. Setting two bottle mouths 62 facilitates adding cells, replacing the culture medium and digesting cells, making the experiment more convenient.

[0054] A plurality of uniformly distributed clamping grooves 221 are formed in the outer ring of the placement groove 22. Two bumps 211 are fixedly installed at positions corresponding to the clamping grooves 221 on the upper surface of the placement plate 21. A first L-shaped blocking rod 23 is rotatably installed between the two bumps 211. The middle part of the first L-shaped blocking rod 23 is rotatably connected to the bump 211 so that the bending direction of the first L-shaped blocking rod 23 can be changed when it rotates, thereby realizing the clamping effect. A torsion spring is fixedly installed between the bump 211 and the first L-shaped blocking rod 23. A sliding groove 212 is formed below each of the plurality of clamping grooves 221. The sliding groove 212 communicates with both the clamping groove 221 and the placement groove 22. A second L-shaped blocking rod 24 is slidably clamped inside the sliding groove 212. One end of the second L-shaped blocking rod 24 has an inclined protrusion 241. A plurality of uniformly distributed pressing springs 26 are fixedly installed between the second L-shaped blocking rod 24 and the sliding groove 212. Pressing plates 25 are provided above both sides of the placement groove 22. The ends of the second L-shaped blocking rods 24 far from the inclined protrusions 241 extend to the outside of the placement plate 21 and are fixedly installed on the lower surfaces of the corresponding pressing plates 25. When the culture bottle 6 is not placed inside the placement groove 22, the first L-shaped blocking rod 23 and the second L-shaped blocking rod 24 are not connected at this time. Only when the culture bottle 6 enters the inside of the placement groove 22, one end of the first L-shaped blocking rod 23 will be hooked by the inclined protrusion 241 under the pushing action of the culture bottle 6 at this time. When the first L-shaped blocking rod 23 is pushed to rotate, one end of it will push the inclined protrusion 241. Because the inclined protrusion 241 is inclined, it will be pushed down. When the end of the first L-shaped blocking rod 23 passes over the inclined protrusion 241, the second L-shaped blocking rod 24 rises under the action of the spring 26, thereby driving the inclined protrusion 241 to rise. The inclined protrusion 241 abuts against one end of the first L-shaped blocking rod 23, thereby realizing the fixation of the first L-shaped blocking rod 23. The inability of the first L-shaped blocking rod 23 to rotate can fix the culture bottle 6 inside the placement groove 22.

[0055] When installing the culture bottle 6, it is only necessary to push the culture bottle 6 into the interior of the placement groove 22. At this time, the annular flat bottle 61 pushes the first L-shaped stop lever 23 to rotate it around the bump 211. At this time, the first L-shaped stop lever 23 is driven to rotate by ninety degrees. One side of the first L-shaped stop lever 23 pushes the inclined protrusion 241. Because the inclined protrusion 241 is inclined, the second L-shaped stop lever 24 will be driven to slide inside the chute 212 under the push of the first L-shaped stop lever 23. Furthermore, the pressing spring 26 is compressed. When the annular flat bottle 61 completely enters the interior of the placement groove 22, at this time, the pressing spring 26 resets and pushes the second L-shaped stop lever 24 and the inclined protrusion 241 to move upward to block the first L-shaped stop lever 23. The other side of the first L-shaped stop lever 23 blocks the annular flat bottle 61. At this time, the installation can be completed. When it is necessary to take it out, only need to press the pressing plate 25, so that the pressing plate 25 drives the second L-shaped stop lever 24 and the inclined protrusion 241 to move downward. At this time, the inclined protrusion 241 loses the locking effect on the first L-shaped stop lever 23. At this time, the annular flat bottle 61 can be taken out. The annular flat bottle 61 will push one side of the first L-shaped stop lever 23 to restore its original position. The torsion spring can keep it in an open state for the next installation.

[0056] By setting the first L-shaped stop lever 23 in this device, it can be automatically locked when installing the culture bottle 6 only by pressing it into the interior of the placement groove 22. When it is necessary to take out the culture bottle 6, only need to press the pressing plate 25 to release the first L-shaped stop lever 23, and then it loses its effect on the culture bottle 6. At this time, only need to pull out the culture bottle 6 from the placement groove 22, which is convenient for installation and removal.

[0057] The working principle of the present invention is as follows:

[0058] During use, first install the culture bottle 6 inside the placement grooves 22 at different positions, and then place the culture bottles 6 of the control group inside the carrier box 34. Start the hydraulic cylinder 38 to contract, and then the placement component 2 is driven to tilt. Control the motor to rotate to drive the rotating plate 35, the telescopic member 36, the collar 37, the hydraulic cylinder 38, and the slider 39 to rotate. Furthermore, the placement component 2 generates a wavy swing, and then a liquid flow is formed inside the culture bottle 6 on the placement component 2, and then a shear stress is generated to imitate the environment inside the organism;

[0059] When installing the culture bottle 6, it is only necessary to push the culture bottle 6 into the interior of the placement groove 22. At this time, the annular flat bottle 61 pushes the first L-shaped stop lever 23 to rotate it around the convex block 211. At this time, the first L-shaped stop lever 23 is driven to rotate by ninety degrees. One side of the first L-shaped stop lever 23 pushes the inclined protrusion 241. Because the inclined protrusion 241 is inclined, the second L-shaped stop lever 24 will be driven to slide inside the chute 212 under the push of the first L-shaped stop lever 23, and then the compression spring 26 is compressed. When the annular flat bottle 61 completely enters the interior of the placement groove 22, at this time, the compression spring 26 resets and pushes the second L-shaped stop lever 24 and the inclined protrusion 241 to move upward to block the first L-shaped stop lever 23. The other side of the first L-shaped stop lever 23 blocks the annular flat bottle 61. At this time, the installation is completed. When it is necessary to take it out, only need to press the pressing plate 25, so that the pressing plate 25 drives the second L-shaped stop lever 24 and the inclined protrusion 241 to move downward. At this time, the inclined protrusion 241 loses the blocking effect on the first L-shaped stop lever 23. At this time, the annular flat bottle 61 can be taken out, and the annular flat bottle 61 will push one side of the first L-shaped stop lever 23 to restore it to its original position.

[0060] The present invention covers any alternatives, modifications, equivalent methods and solutions made on the essence and scope of the present invention. In order to enable the public to have a thorough understanding of the present invention, specific details are described in detail in the following preferred embodiments of the present invention. However, those skilled in the art can fully understand the present invention without the description of these details. In addition, in order to avoid unnecessary confusion to the essence of the present invention, well-known methods, processes, procedures, components and circuits are not described in detail.

[0061] The above are only the preferred embodiments of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A device for cell culture under adjustable fluid shear force, comprising a protective housing, characterized in that: A placement component is installed inside the protection shell, a central connecting piece is installed in the middle of the placement component, and the placement component includes a placement plate; The placement plate is provided with a plurality of groups of annularly distributed slots, each group of slots includes a plurality of placement slots, culture bottles are placed inside the placement slots, a guide rail component is fixedly installed on the lower surface of the placement plate, the central connecting member includes a spherical center member, a lower support rod is fixedly installed below the spherical center member, a rotating plate is rotatably installed on the middle section of the lower support rod, a telescopic rod is fixedly installed on one side of the rotating plate, a collar is fixedly installed on the end of the telescopic rod away from the rotating plate, a hydraulic cylinder is fixedly installed on the lower surface of the collar, the telescopic end of the hydraulic cylinder has a piston rod, the piston rod is slidably clamped in the middle part of the collar, a slider is slidably clamped inside the guide rail component, and the top end of the piston rod is connected to the middle part of the lower surface of the slider through a ball and socket fit; The culture bottle comprises an annular flat bottle for culturing cells. The annular flat bottle is in a circular ring shape, so under the action of the placement component, the culture solution is more likely to flow inside it, and the annular flat bottle is flat. After the culture is completed, the culture bottle is directly placed under a microscope. Bottle openings for placing cells are fixedly installed on both sides of the upper surface of the annular flat bottle. The rotation of the motor drives the rotating plate, the telescopic rod, the collar, the hydraulic cylinder, and the slider to rotate, thereby causing the placement component to swing in a wave shape, thereby causing liquid flow inside the culture bottle on the placement component, thereby generating shear stress; By setting a carrier box, the culture bottle inside the carrier box is used as a control group experiment, so that it can be in the same environment as the culture bottle on the placement component, and only the state of liquid flow changes, ensuring that other factors are the same, ensuring the accuracy of the control experiment, and preventing the external environment from affecting the control group; An upper connecting rod is fixedly installed above the spherical center piece, and a carrying box is fixedly installed at one end of the upper connecting rod away from the spherical center piece, and a culture bottle is also placed inside the carrying box.

2. The device for cell culture under adjustable fluid shear force according to claim 1, characterized in that: The bottle mouth has a detachable card inside with a bottle stopper, the annular flat bottle and the bottle mouth are both made of bioglass, the bottle stopper is made of ordinary glass, and the connection position between the bottle stopper and the bottle mouth is made of frosted glass.

3. The device for cell culture under adjustable fluid shear force according to claim 2, characterized in that: A plurality of evenly distributed card slots are provided at the outer circle of the seating groove, and two protrusions are fixedly installed at positions corresponding to the card slots on the upper surface of the seating plate, and a first L-shaped baffle is rotatably installed between the two protrusions, and a sliding groove is provided under the plurality of card slots, and a second L-shaped baffle is slidingly provided inside the sliding groove, and one end of the second L-shaped baffle has an oblique protrusion, and a plurality of evenly distributed pressing springs are fixedly installed between the second L-shaped baffle and the sliding groove, and pressing plates are provided above both sides of the seating groove, and one end of the second L-shaped baffle away from the oblique protrusion extends to the outside of the seating plate and is fixedly installed on the corresponding lower surface of the pressing plate.

4. The device for cell culture under adjustable fluid shear force according to claim 3, characterized in that: A ball-and-socket type kit is fixedly installed in the middle of the placement plate, and the ball-and-socket type kit is sleeved on the outer surface of the spherical center piece through a ball-and-socket matching sleeve.

5. The device for cell culture under adjustable fluid shear force according to claim 3, characterized in that: The outer circle of the carrying box is fixedly mounted with evenly distributed fixing rods, and one end of the fixing rods away from the carrying box is fixedly mounted on the inner circle of the protective shell.

6. The device for cell culture under adjustable fluid shear force according to claim 5, characterized in that: A plurality of spring telescopic rods distributed in a ring shape are fixedly mounted on the inner lower surface of the protection shell, and a connecting plate is mounted on the telescopic end of the spring telescopic rod, and the connecting plate is slidably mounted on the lower surface of the placement plate.

7. The device for cell culture under controllable fluid shear force according to claim 6, characterized in that: A torsion spring is fixedly installed between the protrusion and the first L-shaped blocking rod.

8. The device for cell culture under controllable fluid shear force according to claim 7, characterized in that: A sealing cover plate is installed at the opening of the protection shell, and a supporting base is fixedly installed at the bottom end of the protection shell.

9. The device for cell culture under controllable fluid shear force according to claim 8, characterized in that: A motor is fixedly mounted on the support base, and a driving end of the motor penetrates the protective shell and the lower support rod and is fixedly mounted on the middle part of the rotating plate.

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