Active oxygen concentration control method and brain-computer interface implant accelerated life test system

Abstract

The invention relates to an active oxygen concentration control method and a brain-computer interface implant accelerated life test system. The accelerated life test system is used for testing the electrochemical performance of a brain-computer interface implant by using a test solution. The method comprises the following steps: detecting a current feedback current corresponding to a current test solution; obtaining the current active oxygen concentration according to the current feedback current and a preset active oxygen concentration and current relation function; generating a filling parameter according to a concentration difference value between the current active oxygen concentration and a preset active oxygen concentration; and adding a hydrogen peroxide solution into the current test solution according to the filling parameters, so that the current test solution reaches the preset active oxygen concentration. According to the method, the active oxygen environment generated by immune reaction in a living body can be simulated through the test solution, the active oxygen concentration in the test solution can be precisely regulated and controlled, and the accuracy of the test result of the brain-computer interface implant is improved.

Description

Technical Field

[0001] This application mainly relates to the field of brain-computer interface implant testing technology, specifically to a method for controlling reactive oxygen species concentration and a brain-computer interface implant accelerated life testing system. Background Technology

[0002] In vitro lifespan testing of brain-computer interface implants is a common method for simulating physiological environments and assessing the expected lifespan of implants, and is crucial for the safety and effectiveness of medical devices. To shorten the testing cycle, accelerated lifespan testing methods based on the relationship between temperature and material reaction rates are typically used.

[0003] Reactive oxygen species (ROS) generated by the immune response in vivo can accelerate the aging of implants, and current accelerated lifespan testing methods do not take into account the impact of ROS. For high-risk devices such as invasive brain-computer interface (BCI) implants, temperature control alone is insufficient to meet the accuracy requirements of in vitro assessment. Existing BCI implant testing methods suffer from inaccurate results. Summary of the Invention

[0004] The technical problem to be solved by this application is to provide a method for controlling reactive oxygen species concentration and a brain-computer interface implant accelerated life testing system. The system can simulate the reactive oxygen species environment generated by the immune response in the body through the test solution, and can accurately control the reactive oxygen species concentration in the test solution, thereby improving the accuracy of brain-computer interface implant test results.

[0005] The technical solution adopted in this application to solve the above-mentioned technical problems is a method for controlling reactive oxygen species (ROS) concentration, applicable to an accelerated life testing system for brain-computer interface (BCI) implants. The accelerated life testing system is used to test the electrochemical performance of the BCI implant using a test solution. The method includes: detecting the current feedback current corresponding to the current test solution; obtaining the current ROS concentration based on the current feedback current and a preset ROS concentration-current relationship function; generating a dispensing parameter based on the concentration difference between the current ROS concentration and the preset ROS concentration; and adding hydrogen peroxide solution to the current test solution according to the dispensing parameter, thereby bringing the current test solution to the preset ROS concentration.

[0006] In one embodiment of this application, the accelerated life testing system includes an electrochemical detection device, which includes a counter electrode and a working electrode. The preset reactive oxygen species (ROS) concentration versus current relationship function is constructed using the following steps: Step Sa: Obtain a preset concentration from a preset ROS concentration range as the current preset concentration, prepare a test solution that conforms to the current preset concentration, and immerse the counter electrode and the working electrode in the test solution; Step Sb: Control the counter electrode to cyclically output a square wave voltage within a first preset duration; Step Sc: Acquire the current value corresponding to the square wave voltage through the working electrode; Step Sd: Filter the current value using a filtering function to obtain the feedback current within a second preset duration before the high level ends, where the second preset duration is less than the first preset duration; Repeat steps Sa to Sd until all preset concentrations within the preset ROS concentration range have been traversed; Step Se: Perform data fitting processing on each preset concentration in the preset ROS concentration range and the corresponding feedback current to obtain the preset ROS concentration versus current relationship function.

[0007] In one embodiment of this application, the preset reactive oxygen species concentration-current relationship function includes a curve function, wherein the reactive oxygen species concentration monotonically increases with the increase of the feedback current.

[0008] In one embodiment of this application, the curve function is constructed as follows: the preset reactive oxygen species concentration range is divided into a first concentration interval, a second concentration interval, and a third concentration interval in ascending order of concentration; an exponential fitting algorithm is used to fit the corresponding preset concentration and feedback current in the first concentration interval to obtain a first sub-curve function; a linear fitting algorithm is used to fit the corresponding preset concentration and feedback current in the second concentration interval to obtain a second sub-curve function; a polynomial fitting algorithm is used to fit the corresponding preset concentration and feedback current in the third concentration interval to obtain a third sub-curve function; and a smooth transition algorithm is used to connect the first sub-curve function, the second sub-curve function, and the third sub-curve function to form the curve function.

[0009] In one embodiment of this application, step Sb: controlling the counter electrode to cyclically output a square wave voltage within a first preset duration includes: step Sb1: controlling the counter electrode to output a square wave voltage of a first preset volt within a third preset duration; step Sb2: controlling the counter electrode to output a square wave voltage of a second preset volt within a fourth preset duration; repeating steps Sb1 and Sb2 until the sum of all third preset durations and all fourth preset durations is greater than or equal to the first preset duration.

[0010] In one embodiment of this application, the accelerated life testing system includes an active oxygen injection device equipped with a water pump, the two ends of which are respectively connected to the test solution and the hydrogen peroxide solution; the injection parameters include injection volume and injection rate; generating injection parameters based on the concentration difference between the current active oxygen concentration and the preset active oxygen concentration includes: calculating a control signal using a proportional-integral-derivative control method based on the concentration difference; and generating the injection volume and injection rate based on the control signal, the concentration parameter of the hydrogen peroxide solution, and the flow rate parameter of the water pump.

[0011] In one embodiment of this application, adding hydrogen peroxide solution to the current test solution according to the addition parameters includes: instructing the water pump to add the hydrogen peroxide solution to the test solution according to the addition amount and the addition speed in response to the current reactive oxygen species concentration being less than the preset reactive oxygen species concentration; or instructing the water pump to stop adding the hydrogen peroxide solution to the test solution in response to the current reactive oxygen species concentration being greater than or equal to the preset reactive oxygen species concentration.

[0012] In one embodiment of this application, the test solution includes salt; the method further includes: detecting the current liquid level height corresponding to the current test solution; in response to the current liquid level height being less than a first preset height, adding purified water to the current test solution to make the current liquid level height reach the first preset height, thereby making the salt concentration in the current test solution meet a preset salt concentration; and / or in response to the current liquid level height being less than a second preset height, stopping heating the current test solution, the second preset height being less than the first preset height.

[0013] In one embodiment of this application, the method further includes: detecting the current solution temperature corresponding to the current test solution; in response to the current solution temperature being less than a preset solution temperature, heating the current test solution until the current solution temperature reaches the preset solution temperature, and dynamically adjusting the heating power based on the temperature difference between the current solution temperature and the preset solution temperature during the heating process; or in response to the current solution temperature being greater than the preset solution temperature, stopping the heating of the current test solution so that the current solution temperature reaches the preset solution temperature.

[0014] To address the aforementioned technical problems, this application also proposes an accelerated lifespan testing system for brain-computer interface implants, comprising: an implant testing device for housing the brain-computer interface implant and testing its electrochemical performance using a test solution; an electrochemical detection device including a counter electrode and a working electrode, wherein the counter electrode is used to output a square wave voltage and the working electrode is used to acquire the current value corresponding to the square wave voltage; an active oxygen (OO) dispensing device for dispensing hydrogen peroxide solution; a liquid level sensor for detecting the liquid level height corresponding to the test solution; a purified water dispensing device for dispensing purified water; a temperature sensor for detecting the solution temperature corresponding to the test solution; a heating device for heating the test solution; and a processor configured to adjust the test solution using the active OO concentration control method described above.

[0015] The technical solution of this application accurately obtains the current reactive oxygen species (ROS) concentration by detecting the current feedback current of the test solution and combining it with a preset ROS concentration-current relationship function. Then, it generates dosing parameters based on the concentration difference and adds hydrogen peroxide solution, thereby achieving dynamic control of ROS concentration. This allows the test solution to be stably maintained at the preset ROS concentration, effectively simulating the ROS environment generated by the immune response in the body during the accelerated life test of brain-computer interface implants. This provides stable test conditions for the electrochemical performance testing of implants and improves the accuracy and reliability of brain-computer interface implant life assessment results. Attached Figure Description

[0016] To make the above-mentioned objectives, features and advantages of this application more apparent and understandable, the specific embodiments of this application will be described in detail below with reference to the accompanying drawings, wherein: Figure 1 This is a schematic diagram of an accelerated life testing system for a brain-computer interface implant according to an embodiment of this application; Figure 2 This is a partial schematic diagram of an accelerated life testing system for a brain-computer interface implant according to one embodiment of this application; Figure 3 This is a flowchart of a reactive oxygen species concentration control method according to an embodiment of this application; Figure 4 This is a graph showing the relationship between time and voltage when the electrodes cyclically output a square wave voltage in one embodiment of this application; Figure 5 This is a schematic diagram of the pre-defined function relating reactive oxygen species concentration to current in one embodiment of this application; Figure 6 This is a system block diagram of a reactive oxygen species concentration control system according to an embodiment of this application.

[0017] Explanation of reference numerals in the accompanying drawings for specific embodiments: 10. Accelerated life testing system; 11. Implant testing device; 111. Test solution; 112. Hydrogen peroxide solution; 12. Electrochemical detection device; 121. Counter electrode; 122. Working electrode; 123. Electrochemical workstation; 13. Active oxygen injection device; 131. Water pump; 132. Controller; 14. Purified water dispensing device. Detailed Implementation

[0018] To make the above-mentioned objectives, features and advantages of this application more apparent and understandable, the specific embodiments of this application will be described in detail below with reference to the accompanying drawings.

[0019] Many specific details are set forth in the following description in order to provide a full understanding of this application. However, this application may also be implemented in other ways different from those described herein, and therefore this application is not limited to the specific embodiments disclosed below.

[0020] As illustrated in this application, unless the context clearly indicates otherwise, the words "a," "an," "an," and / or "the" do not specifically refer to the singular and may also include the plural. Generally speaking, the terms "comprising" and "including" only indicate the inclusion of explicitly identified steps and elements, which do not constitute an exclusive list, and the method or apparatus may also include other steps or elements.

[0021] Flowcharts are used in this application to illustrate the operations performed by the system according to embodiments of this application. It should be understood that the preceding or following operations are not necessarily performed in exact order. Instead, various steps can be processed in reverse order or simultaneously. Furthermore, other operations may be added to these processes, or one or more steps may be removed from these processes.

[0022] This application uses hydrogen peroxide as a reactive oxygen species (ROS) source to simulate the effects of immune responses in vivo during the testing of brain-computer interface (BCI) implants. This application identifies potential problems with adding hydrogen peroxide to the test solution, including: firstly, hydrogen peroxide is easily decomposed and volatilized at room temperature and under heating conditions, making it impossible to maintain a stable ROS concentration in the test solution, which may interfere with the analysis of electrochemical performance; secondly, the lack of a coordinated control mechanism for liquid level, temperature, and ROS concentration may also affect the accuracy and reliability of the BCI implant test results. To overcome these problems, this application designs a ROS concentration control scheme for the test solution in a BCI implant accelerated lifespan testing system.

[0023] First, we will introduce the accelerated life testing system for the brain-computer interface implant of this application. The reactive oxygen species concentration control method of this application will be explained later.

[0024] Figure 1 This is a schematic diagram of an accelerated life testing system for a brain-computer interface implant according to an embodiment of this application. (Reference) Figure 1 As shown, the accelerated life testing system 10 for brain-computer interface implants in this embodiment includes: an implant testing device 11, an electrochemical detection device 12, a reactive oxygen species (ROS) injection device 13, a liquid level sensor (not shown), a purified water injection device 14, a temperature sensor (not shown), a heating device (not shown), and a processor (not shown). The processor is configured to execute the ROS concentration control method described later to adjust the test solution. Specifically, the implant testing device 11 is used to house a brain-computer interface implant (not shown) and to test the electrochemical performance of the brain-computer interface implant using the test solution.

[0025] Figure 2 This is a partial schematic diagram of an accelerated life testing system for a brain-computer interface implant according to one embodiment of this application. (Reference) Figure 1 and Figure 2 As shown, the electrochemical detection device 12 includes a counter electrode 121 and a working electrode 122. The counter electrode 121 is used to output a square wave voltage, and the working electrode 122 is used to acquire the current value corresponding to the square wave voltage. For example, the electrochemical detection device 12 also includes an electrochemical workstation 123. The counter electrode 121 and the working electrode 122 are immersed in a test solution 111. The electrochemical workstation 123 is capable of controlling the output voltage and acquiring the feedback current. Figure 2 In the diagram, R corresponds to the reference electrode, which provides a stable reference potential; W corresponds to the working electrode 122, which is the electrode where the target electrochemical reaction occurs; and C corresponds to the counter electrode 121, which forms a current loop with the working electrode 122 to assist in the electrochemical reaction.

[0026] Active oxygen injection device 13 (equipped with such as Figure 2 The water pump 131 shown is used to add hydrogen peroxide solution 112. Exemplarily, the water pump 131 can be controlled by the controller 132 to draw hydrogen peroxide solution 112 into the test solution 111. A level sensor is used to detect the liquid level height corresponding to the test solution 111. A purified water dispensing device 14 is used to add purified water. A temperature sensor is used to detect the solution temperature corresponding to the test solution 111. A heating device is used to heat the test solution 111.

[0027] For example, the test solution 111 of this application is composed of water (such as purified water), salt (such as phosphate), and hydrogen peroxide. The accelerated life testing system 10 for brain-computer interface implants of this application provides a stable test carrier through the implant testing device 11; the counter electrode 121 of the electrochemical detection device 12 outputs a square wave voltage, and the working electrode 122 collects the current value. In conjunction with the active oxygen infusion device 13, hydrogen peroxide solution 112 is added. The liquid level and salt concentration of the test solution 111 are maintained according to the liquid level sensor and the purified water infusion device 14; the temperature of the test solution 111 is accurately controlled through the temperature sensor and the heating device; and the active oxygen concentration control method of this application is executed by the processor, realizing a three-way cyclic coordinated control scheme of active oxygen concentration, liquid level, and solution temperature.

[0028] This application can simulate the reactive oxygen species environment generated by the immune response in a living organism, providing a stable, reliable and safe accelerated life testing environment for brain-computer interface implants that is close to physiological reality, thereby improving the accuracy and reliability of implant life test results.

[0029] The method for controlling reactive oxygen species concentration in this application will be described later.

[0030] This application proposes a method for controlling reactive oxygen species (ROS) concentration, applicable to scenarios where the electrochemical performance of a brain-computer interface (BCI) implant is tested using a test solution in an accelerated life testing system. The ROS concentration control method can run locally on the accelerated life testing system, such as within the system's controller or processor, or on a cloud platform. When running on a cloud platform, data from the local accelerated life testing system interacts with cloud platform data via a wireless network. Exemplarily, the cloud platform may include a private cloud, public cloud, hybrid cloud, community cloud, distributed cloud, interconnected cloud, and multiple clouds, or any combination thereof. This application does not limit the operating environment of the ROS concentration control method.

[0031] Figure 3 This is a flowchart of a reactive oxygen species concentration control method according to an embodiment of this application. (Reference) Figure 3 As shown, the reactive oxygen species concentration control method of this embodiment includes the following steps: Step S110: Detect the current feedback current corresponding to the current test solution.

[0032] Step S120: Obtain the current reactive oxygen species concentration based on the current feedback current and the preset reactive oxygen species concentration-current relationship function.

[0033] Step S130: Generate injection parameters based on the concentration difference between the current reactive oxygen species concentration and the preset reactive oxygen species concentration.

[0034] Step S140: Add hydrogen peroxide solution to the current test solution according to the addition parameters, so that the current test solution reaches the preset active oxygen concentration.

[0035] The following details steps S110 to S140 described above: In step S110, the current feedback current corresponding to the current test solution is detected. For example, refer to... Figure 1 and Figure 2 As shown, the feedback current of the test solution 111 can be detected by the electrochemical detection device 12.

[0036] In step S120, the current reactive oxygen species (ROS) concentration is obtained based on the current feedback current and a preset ROS concentration-current relationship function. For example, the preset ROS concentration-current relationship function can be constructed in advance through experiments.

[0037] Continue to refer to Figure 1 and Figure 2 As shown, in some embodiments, the accelerated lifetime testing system 10 includes an electrochemical detection device 12, which includes a counter electrode 121 and a working electrode 122; the preset reactive oxygen species concentration versus current relationship function is constructed using the following operation: Step Sa: Obtain a preset concentration (such as 0mM, 1mM, or 2mM) from the preset active oxygen concentration range (such as 0mM~100mM, where mM represents millimoles per liter) as the current preset concentration, prepare a test solution 111 that conforms to the current preset concentration, and immerse the counter electrode 121 and the working electrode 122 into the test solution 111. Step Sb: Control the counter electrode 121 to cyclically output square wave voltage within a first preset duration (e.g., 20s, where s represents seconds); Step Sc: Acquire the current value corresponding to the square wave voltage through the working electrode 122; Step Sd: Use a filtering function to filter the current value to obtain the feedback current within the second preset time (e.g., 1s~2s) before the high level ends. The second preset time is less than the first preset time. Repeat steps Sa~Sd until all preset concentrations within the preset active oxygen concentration range have been traversed. Step Se: Perform data fitting processing on each preset concentration in the preset reactive oxygen species concentration range and the corresponding feedback current to obtain the preset reactive oxygen species concentration and current relationship function.

[0038] Figure 4 This is a graph showing the relationship between time and voltage when a square wave voltage is cyclically output from the electrodes in one embodiment of this application. (Reference) Figure 4As shown, for example, in step Sb above, the cyclic output parameters of the square wave voltage can be set to V1 volts for t1 seconds and V2 volts for t2 seconds, for example, V1=-800mV (mV represents millivolts), t1=1s, V2=850mV, t2=4s, and the first preset duration is the total duration of the cyclic output (e.g., 20s). In step Sd above, the interference noise of the signal corresponding to the current value can be eliminated through the filtering function.

[0039] This application completes multiple sets of data acquisition and fitting by traversing the preset reactive oxygen species concentration range through the aforementioned operations. The constructed reactive oxygen species concentration and current relationship function can accurately reflect the correspondence between the reactive oxygen species concentration and the feedback current in the test solution, providing a reliable data basis for the real-time detection of reactive oxygen species concentration.

[0040] Continue to refer to Figure 2 and Figure 4 As shown, in some embodiments, step Sb: controlling the counter electrode 121 to cyclically output a square wave voltage within a first preset duration (e.g., 20 seconds) includes: Step Sb1: Control the counter electrode 121 to output a square wave voltage of the first preset volt (e.g., the first preset volt V1 = -800mV) within the third preset time period (e.g., the third preset time period t1 = 1s); Step Sb2: Control the counter electrode 121 to output a square wave voltage of the second preset volt (e.g., the second preset volt V2 = 850mV) within the fourth preset duration (e.g., the fourth preset duration t2 = 4s); repeat steps Sb1 and Sb2 until the sum of all third preset durations and all fourth preset durations is greater than or equal to the first preset duration.

[0041] For example, this application ensures that the working electrode 122 can acquire a stable current signal by continuously cyclically outputting a square wave voltage, thus guaranteeing the reliability of the feedback current.

[0042] Figure 5 This is a schematic diagram illustrating the pre-defined relationship between reactive oxygen species concentration and current in one embodiment of this application. (Reference) Figure 5 As shown, in some embodiments, the preset function relating reactive oxygen species concentration to current includes a curve function, wherein the reactive oxygen species concentration C monotonically increases with increasing feedback current A. For example, Figure 5 The curve shown indicates that the value of the feedback current A increases as the reactive oxygen species concentration C increases from 0 mM to 100 mM.

[0043] In some embodiments, the curve function is constructed in the following manner: The preset active oxygen concentration range (e.g., 0mM~100mM) is divided into a first concentration range (e.g., 0mM~20mM), a second concentration range (e.g., 20mM~60mM), and a third concentration range (e.g., 60mM~100mM) in ascending order of concentration. In the first concentration range, an exponential fitting algorithm is used to fit the corresponding preset concentration and feedback current to obtain the first sub-curve function; In the second concentration range, a linear fitting algorithm is used to fit the corresponding preset concentration and feedback current to obtain the second sub-curve function; In the third concentration range, a polynomial fitting algorithm is used to fit the corresponding preset concentration and feedback current to obtain the third sub-curve function. A smooth transition algorithm is used to connect the first sub-curve function, the second sub-curve function, and the third sub-curve function to form a complete curve function.

[0044] For example, the first concentration range can be a low concentration range, corresponding to the reaction rate-limited stage, where the feedback current may increase non-linearly, so an exponential fitting algorithm can be used; the second concentration range can be a medium concentration range, corresponding to the reaction rate-stable stage, where the reactive oxygen concentration and the feedback current have an approximately linear relationship, so a linear fitting algorithm can be used; the third concentration range can be a high concentration range, corresponding to the reaction rate-saturated stage, where the feedback current may increase more slowly, so a polynomial fitting algorithm can be used; the smooth transition algorithm can eliminate the fitting discontinuities of the three types of sub-curve functions at the interval boundaries, ensuring the continuity and monotonicity of the entire curve function, and improving the fitting accuracy of the curve function.

[0045] In step S130, injection parameters are generated based on the concentration difference between the current reactive oxygen species concentration and the preset reactive oxygen species concentration.

[0046] refer to Figure 1 and Figure 2 As shown, in some embodiments, the accelerated life testing system 10 includes an active oxygen injection device 13 equipped with a water pump 131, with the two ends of the water pump 131 connected to a test solution 111 and a hydrogen peroxide solution 112, respectively; the injection parameters include injection volume and injection speed; the injection parameters are generated based on the concentration difference between the current active oxygen concentration and the preset active oxygen concentration, including: The control signal is calculated using the proportional-integral-derivative (PID) control method based on the concentration difference. The injection volume and injection rate are generated based on the control signal, the concentration parameters of the hydrogen peroxide solution, and the flow parameters of the water pump.

[0047] For example, the flow rate parameter of the water pump can be the delivery volume per unit time; the concentration parameter of the hydrogen peroxide solution can be set to 10mM~30mM. This application uses a PID controller based on the current reactive oxygen species concentration C and a preset reactive oxygen species concentration C. SET The control signal is dynamically calculated based on the concentration difference between the two solutions. Combined with the flow parameters of the water pump and the concentration parameters of the hydrogen peroxide solution, the required amount of hydrogen peroxide to be added (i.e., the total amount of hydrogen peroxide solution needed to compensate for the concentration difference) and the addition rate (i.e., the amount added per unit time) can be accurately calculated. This application, through PID control and multi-parameter collaborative calculation, can generate accurate addition parameters, avoiding concentration fluctuations caused by over- or under-addition. Through the stable delivery of water pump 131, the smooth addition of hydrogen peroxide solution 112 is achieved, ensuring the stability of the active oxygen concentration in test solution 111.

[0048] In step S140, hydrogen peroxide solution is added to the current test solution according to the addition parameters, thereby bringing the current test solution to a preset active oxygen concentration. (Reference) Figure 2 As shown, in some embodiments, it includes: In response to the current reactive oxygen species concentration C being less than the preset reactive oxygen species concentration C SET The water pump 131 is instructed to add hydrogen peroxide solution 112 to the test solution 111 according to the injection volume and injection speed; Alternatively, in response to the current reactive oxygen species concentration C being greater than or equal to the preset reactive oxygen species concentration C... SET The water pump 131 is instructed to stop adding hydrogen peroxide solution 112 to the test solution 111.

[0049] For example, step S140 is equivalent to "reactive oxygen species concentration cycling logic", when the electrochemical detection device detects C < C SET If the concentration of reactive oxygen species in the test solution is insufficient, hydrogen peroxide solution needs to be added using a water pump at the calculated injection rate and volume. When C ≥ C... SET When the concentration of reactive oxygen species (ROS) meets the testing requirements, the pump is instructed to stop adding water to prevent excessive ROS concentration. This application enables on-demand replenishment of hydrogen peroxide solution, avoiding excessive or insufficient ROS concentration, ensuring that the ROS concentration in the test solution is always maintained within a suitable range, and guaranteeing the stability of the testing environment.

[0050] In some embodiments, the test solution includes a salt, such as phosphate-buffered saline (PBS). The reactive oxygen species concentration control method further includes: Detect the current liquid level height of the current test solution; In response to the current liquid level being less than the first preset height, purified water is added to the current test solution to bring the current liquid level to the first preset height, thereby ensuring that the salt concentration in the current test solution meets the preset salt concentration. And / or, in response to the current liquid level being less than a second preset height, stop heating the current test solution, where the second preset height is less than a first preset height.

[0051] For example, the above steps are equivalent to "liquid level sensor loop logic". The test solution in this application consists of water, salt, and hydrogen peroxide. The first preset height Lup is the upper limit of the preset liquid level, and the second preset height Low is the lower limit of the preset liquid level. During the electrochemical performance testing of the brain-computer interface implant, water will evaporate, causing the liquid level of the test solution to drop, which in turn increases the salt concentration and affects the accuracy of electrochemical signal acquisition. Therefore, when the liquid level is lower than the first preset height Lup, purified water is added through a purified water adding device until the liquid level is restored to the first preset height Lup to maintain a constant salt concentration. To avoid the risk of dry burning, when the liquid level is lower than the second preset height Low (warning lower limit), the heating device is controlled to stop heating and an alarm signal is issued.

[0052] This application can maintain a constant salt concentration in the test solution, avoid abnormal salt concentration from interfering with electrochemical signal acquisition and active oxygen concentration detection, and also realize dry burning protection and alarm functions, thereby improving the safety of the test process.

[0053] In some embodiments, the reactive oxygen species concentration control method further includes: Detect the current temperature of the current test solution; In response to the current solution temperature being lower than the preset solution temperature, the current test solution is heated until the current solution temperature reaches the preset solution temperature. During the heating process, the heating power is dynamically adjusted based on the temperature difference between the current solution temperature and the preset solution temperature. Alternatively, in response to the current solution temperature being higher than the preset solution temperature, heating of the current test solution is stopped so that the current solution temperature reaches the preset solution temperature.

[0054] For example, the above steps are equivalent to a "temperature sensor loop logic." When the temperature sensor detects that the current solution temperature is lower than the preset solution temperature, the heating device is activated and the heating power is dynamically adjusted according to the temperature difference: for example, when the temperature difference is large, high-power heating is used; when the temperature difference is small, low-power heating is used to avoid a sudden temperature rise. When the current solution temperature is higher than the preset solution temperature, the heating device is stopped to allow the temperature to drop back to the preset solution temperature.

[0055] The temperature control in this application is based on water bath heating and PID algorithm control. The preset solution temperature can be set within the range of 20℃ to 87℃. This temperature range can meet the high-temperature requirements of accelerated life testing of brain-computer interface implants, thereby enabling implant life testing at a rate up to 32 times faster. This application can achieve precise temperature control of the test solution, avoiding rapid decomposition of hydrogen peroxide due to temperature fluctuations, and providing a good environmental basis for the stability of reactive oxygen species concentration. At the same time, through the coordinated operation of temperature control, reactive oxygen species concentration control, and liquid level control, a stable test environment is constructed, which can meet the requirements of accelerated life testing of brain-computer interface implants.

[0056] refer to Figure 1 and Figure 2 As shown below, a complete embodiment is used to describe the accelerated life testing process of the brain-computer interface implant of this application.

[0057] 1. Pre-configuration stage. Add a test solution 111 containing salt to the implant testing device 11, inject purified water through the purified water injection device 14, and use a liquid level sensor to detect the current liquid level height until the liquid level reaches the first preset height to complete the pre-configuration.

[0058] 2. Electrochemical Performance Testing Stage. Preset parameters include active oxygen concentration, solution temperature, first preset height, second preset height, and test duration. Hydrogen peroxide solution 112 is added to the test solution 111 via the active oxygen addition device 13 to create a test environment containing active oxygen. The heating device is activated to heat the test solution 111, and the current solution temperature is detected by a temperature sensor.

[0059] 3. Monitoring cycle and test environment maintenance phase.

[0060] (1) Cyclic control of reactive oxygen species (ROS) concentration is performed. The counter electrode 121 of the electrochemical detection device 12 cyclically outputs a square wave voltage, and the working electrode 122 collects the corresponding current value. The current value is processed by a filtering function to obtain the feedback current. Combined with the preset ROS concentration and current relationship function, the current ROS concentration is obtained. Based on the concentration difference between the current ROS concentration and the preset ROS concentration, the PID control method is used to generate the injection parameters. If the current ROS concentration is less than the preset ROS concentration, the water pump 131 is instructed to add hydrogen peroxide solution 112 to the test solution 111 according to the injection parameters; if the current ROS concentration is greater than or equal to the preset ROS concentration, the water pump 131 is instructed to stop adding hydrogen peroxide solution 112.

[0061] (2) Perform liquid level sensor cycle control. The liquid level sensor continuously detects the current liquid level of the test solution 111. If the current liquid level is less than the first preset height, purified water is added to the test solution 111 through the purified water adding device 14 to make the liquid level reach the first preset height and maintain a constant salt concentration.

[0062] (3) Perform temperature sensor cycle control. The current solution temperature of the test solution 111 is continuously detected by the temperature sensor. If the current solution temperature is lower than the preset solution temperature, the test solution 111 is heated and the heating power is dynamically adjusted according to the temperature difference; if the current solution temperature is higher than the preset solution temperature, the heating of the test solution 111 is stopped.

[0063] 4. Safety Protection Phase. The current liquid level of the test solution 111 is continuously monitored by a liquid level sensor. If the current liquid level is lower than the second preset height, heating of the test solution 111 is immediately stopped and an alarm signal is issued.

[0064] 5. Electrochemical performance test completion stage. When the test duration reaches the preset time, the test is stopped, and the heating device and all filling devices are shut down. The test solution 111 is drained through the draining device.

[0065] This application can produce the following beneficial effects: (1) Closed-loop control of reactive oxygen species concentration was achieved. By combining electrochemical detection with PID replenishment control, the reactive oxygen species concentration in the test solution can be stably maintained, thus improving the accuracy of the test results.

[0066] (2) It effectively simulates the immune environment in the body. By introducing reactive oxygen species, it breaks through the limitation of traditional tests that rely solely on temperature control, making the test environment closer to the real physiological immune state in the body, and improving the reliability of implant life assessment.

[0067] (3) It can be adapted to high temperature accelerated testing scenarios. This application supports testing environments such as 20℃~87℃, and can achieve implant life testing at a rate of up to 32 times, which can meet the actual needs of rapid implant life assessment.

[0068] This application also includes a reactive oxygen species (ROS) concentration control system, comprising a memory and a processor. The memory stores instructions executable by the processor; the processor executes these instructions to implement the ROS concentration control method described above.

[0069] Figure 6 This is a system block diagram of a reactive oxygen species concentration control system according to an embodiment of this application. (Reference) Figure 6As shown, the reactive oxygen species (ROS) concentration control system 600 may include an internal communication bus 601, a processor 602, a read-only memory (ROM) 603, a random access memory (RAM) 604, and a communication port 605. The ROS concentration control system 600 may also include a hard disk 606. The internal communication bus 601 enables data communication between the components of the ROS concentration control system 600. The processor 602 can make judgments and issue prompts. In some embodiments, the processor 602 may consist of one or more processors. The communication port 605 enables data communication between the ROS concentration control system 600 and external devices. In some embodiments, the ROS concentration control system 600 can send and receive information and data from a network through the communication port 605. The ROS concentration control system 600 may also include different forms of program storage units and data storage units, such as the hard disk 606, the read-only memory (ROM) 603, and the random access memory (RAM) 604, capable of storing various data files used for computer processing and / or communication, as well as possible program instructions executed by the processor 602. The processor executes these instructions to implement the main part of the method. The results processed by the processor are transmitted to the user device through the communication port and displayed on the user interface.

[0070] The above-described reactive oxygen species concentration control method can be implemented as a computer program, stored in hard disk 606, and loaded into processor 602 for execution to implement the reactive oxygen species concentration control method of this application.

[0071] This application also includes a computer-readable medium storing computer program code that, when executed by a processor, implements the reactive oxygen species concentration control method described above.

[0072] When a reactive oxygen species (ROS) concentration control method is implemented as a computer program, it can also be stored as an article of manufacture in a computer-readable storage medium. For example, a computer-readable storage medium may include, but is not limited to, magnetic storage devices (e.g., hard disks, floppy disks, magnetic stripes), optical discs (e.g., compact discs (CDs), digital multifunction discs (DVDs)), smart cards, and flash memory devices (e.g., electrically erasable programmable read-only memory (EPROM), cards, sticks, key drives). Furthermore, the various storage media described herein can represent one or more devices and / or other machine-readable media for storing information. The term "machine-readable medium" may include, but is not limited to, wireless channels and various other media (and / or storage media) capable of storing, containing, and / or carrying code and / or instructions and / or data.

[0073] It should be understood that the embodiments described above are merely illustrative. The embodiments described herein may be implemented in hardware, software, firmware, middleware, microcode, or any combination thereof. For hardware implementation, the processor may be implemented within one or more application-specific integrated circuits (ASICs), digital signal processors (DSPs), digital signal processing devices (DSPDs), programmable logic devices (PLDs), field-programmable gate arrays (FPGAs), processors, controllers, microcontrollers, microprocessors, and / or other electronic units designed to perform the functions described herein, or combinations thereof.

[0074] Some aspects of this application can be executed entirely by hardware, entirely by software (including firmware, resident software, microcode, etc.), or by a combination of hardware and software. The aforementioned hardware or software may be referred to as a "data block," "module," "engine," "unit," "component," or "system." The processor may be one or more application-specific integrated circuits (ASICs), digital signal processors (DSPs), digital signal processing devices (DAPDs), programmable logic devices (PLDs), field-programmable gate arrays (FPGAs), processors, controllers, microcontrollers, microprocessors, or combinations thereof. Furthermore, aspects of this application may manifest as computer products residing in one or more computer-readable media, including computer-readable program code. For example, computer-readable media may include, but are not limited to, magnetic storage devices (e.g., hard disks, floppy disks, magnetic tapes, etc.), optical discs (e.g., compressed CDs, digital multifunction DVDs, etc.), smart cards, and flash memory devices (e.g., cards, sticks, key drives, etc.).

[0075] A computer-readable medium may contain a propagated data signal containing computer program code, for example, on baseband or as part of a carrier wave. This propagated signal may take various forms, including electromagnetic, optical, and so on, or suitable combinations thereof. A computer-readable medium can be any computer-readable medium other than a computer-readable storage medium, which can be connected to an instruction execution system, apparatus, or device to enable communication, propagation, or transmission of a program for use. The program code located on the computer-readable medium can be propagated through any suitable medium, including radio, cable, fiber optic cable, radio frequency signals, or similar media, or any combination of the above media.

[0076] The basic concepts have been described above. Obviously, for those skilled in the art, the above disclosure is merely illustrative and does not constitute a limitation of this application. Although not explicitly stated herein, those skilled in the art may make various modifications, improvements, and corrections to this application. Such modifications, improvements, and corrections are suggested in this application, and therefore remain within the spirit and scope of the exemplary embodiments of this application.

[0077] Furthermore, this application uses specific terms to describe embodiments of the application. For example, "an embodiment," "one embodiment," and / or "some embodiments" refer to a particular feature, structure, or characteristic related to at least one embodiment of the application. Therefore, it should be emphasized and noted that "an embodiment," "one embodiment," or "an alternative embodiment" mentioned twice or more in different locations in this specification do not necessarily refer to the same embodiment. In addition, certain features, structures, or characteristics in one or more embodiments of the application can be appropriately combined.

[0078] In some embodiments, numbers describing the quantity of components and attributes are used. It should be understood that such numbers used to describe embodiments are sometimes modified by the terms "approximately," "approximately," or "generally." Unless otherwise stated, "approximately," "approximately," or "generally" indicates that the numbers are allowed to vary by ±20%. Accordingly, in some embodiments, the numerical parameters used in this application are approximate values, which may be changed depending on the characteristics required by individual embodiments. In some embodiments, numerical parameters should take into account specified significant digits and employ a general method of digit reservation. Although the numerical ranges and parameters used to confirm their breadth of range in some embodiments of this application are approximate values, in specific embodiments, such values ​​are set as precisely as feasible.

Claims

1. A method of controlling the concentration of active oxygen, characterized by, An accelerated life test system suitable for a brain-computer interface implant, the accelerated life test system being used for testing electrochemical performance of the brain-computer interface implant using a test solution; the method comprising: detecting a current feedback current corresponding to a current test solution; obtaining a current active oxygen concentration according to the current feedback current and a preset active oxygen concentration-current relationship function; generating a filling parameter according to a concentration difference between the current active oxygen concentration and a preset active oxygen concentration; adding hydrogen peroxide solution to the current test solution according to the filling parameter, so that the current test solution reaches the preset active oxygen concentration.

2. The active oxygen concentration control method according to claim 1, wherein The accelerated life test system comprises an electrochemical detection device, the electrochemical detection device comprising a counter electrode and a working electrode; the preset active oxygen concentration-current relationship function is constructed by the following operations: Step Sa: obtaining a preset concentration as a current preset concentration from a preset active oxygen concentration range, preparing a test solution conforming to the current preset concentration, and immersing the counter electrode and the working electrode in the test solution; Step Sb: controlling the counter electrode to cyclically output a square wave voltage within a first preset time length; Step Sc: collecting a current value corresponding to the square wave voltage through the working electrode; Step Sd: filtering the current value by using a filtering function to obtain a feedback current within a second preset time length before a high level ends, the second preset time length being less than the first preset time length; repeatedly executing the steps Sa-Sd until all preset concentrations in the preset active oxygen concentration range are traversed; Step Se: performing data fitting processing on each preset concentration in the preset active oxygen concentration range and the corresponding feedback current, thereby obtaining the preset active oxygen concentration-current relationship function.

3. The active oxygen concentration control method according to claim 2, wherein The preset active oxygen concentration-current relationship function comprises a curve function, and the curve function satisfies that the active oxygen concentration monotonically increases with the increase of the feedback current.

4. The active oxygen concentration control method according to claim 3, wherein The curve function is constructed in the following way: dividing the preset active oxygen concentration range into a first concentration interval, a second concentration interval and a third concentration interval in order of concentration from small to large; in the first concentration interval, using an exponential fitting algorithm to fit the corresponding preset concentration and feedback current to obtain a first sub curve function; in the second concentration interval, using a linear fitting algorithm to fit the corresponding preset concentration and feedback current to obtain a second sub curve function; in the third concentration interval, using a polynomial fitting algorithm to fit the corresponding preset concentration and feedback current to obtain a third sub curve function; using a smooth transition algorithm to connect the first sub curve function, the second sub curve function and the third sub curve function to form the curve function.

5. The active oxygen concentration control method according to claim 2, wherein The step Sb: controlling the counter electrode to cyclically output a square wave voltage within a first preset time length, comprises: Step Sb1: controlling the counter electrode to output a square wave voltage of a first preset volt within a third preset time length; Step Sb2: controlling the counter electrode to output a second preset voltage square wave voltage in a fourth preset time length; repeating the step Sb1 and the step Sb2 until the sum of all third preset time lengths and all fourth preset time lengths is greater than or equal to the first preset time length.

6. The active oxygen concentration control method according to claim 1 or 2, characterized by, The accelerated life test system comprises an active oxygen filling device provided with a water pump, two ends of the water pump being communicated with the test solution and the hydrogen peroxide solution respectively; the filling parameters comprise a filling amount and a filling speed; The filling parameters are generated according to a concentration difference between the current active oxygen concentration and a preset active oxygen concentration, comprising: The control signal is calculated using a proportional-integral-derivative control method according to the concentration difference; The filling amount and the filling speed are generated according to the control signal, a concentration parameter of the hydrogen peroxide solution and a flow parameter of the water pump.

7. The active oxygen concentration control method according to claim 6, wherein The hydrogen peroxide solution is added to the current test solution according to the filling parameters, comprising: In response to the current active oxygen concentration being less than the preset active oxygen concentration, the water pump is instructed to add the hydrogen peroxide solution to the test solution according to the filling amount and the filling speed; or In response to the current active oxygen concentration being greater than or equal to the preset active oxygen concentration, the water pump is instructed to stop adding the hydrogen peroxide solution to the test solution.

8. The active oxygen concentration control method according to claim 1, wherein The test solution comprises salt; the method further comprises: Detecting a current liquid level corresponding to the current test solution; In response to the current liquid level being less than a first preset height, adding purified water to the current test solution to make the current liquid level reach the first preset height, so that the salt concentration in the current test solution meets a preset salt concentration; and / or In response to the current liquid level being less than a second preset height, stopping heating the current test solution, the second preset height being less than the first preset height.

9. The active oxygen concentration control method according to claim 1, wherein Further comprising: Detecting a current solution temperature corresponding to the current test solution; In response to the current solution temperature being less than a preset solution temperature, heating the current test solution until the current solution temperature reaches the preset solution temperature, and dynamically adjusting a heating power according to a temperature difference between the current solution temperature and the preset solution temperature during the heating; Or In response to the current solution temperature being greater than the preset solution temperature, stopping heating the current test solution so that the current solution temperature reaches the preset solution temperature.

10. An accelerated life testing system for a brain computer interface implant, comprising: Comprising: An implant test device for accommodating the brain-computer interface implant and testing the electrochemical performance of the brain-computer interface implant using a test solution; An electrochemical detection device comprising a counter electrode and a working electrode, the counter electrode being used to output a square wave voltage, and the working electrode being used to collect a current value corresponding to the square wave voltage; An active oxygen filling device for filling hydrogen peroxide solution; A liquid level sensor for detecting a liquid level corresponding to the test solution; A purified water filling device for filling purified water; A temperature sensor for detecting a solution temperature corresponding to the test solution; A heating device for heating the test solution; a processor configured to adjust the test solution using the active oxygen concentration control method of any of claims 1-9.

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