An in-situ stratified culture device and method for improving carbon and nitrogen fixation rates in the open ocean.

Abstract

This invention discloses an in-situ stratified culture device and method for improving carbon and nitrogen fixation rates in the ocean, relating to the field of ocean carbon and nitrogen fixation technology, including a feeding cylinder. The device and method disclosed in this invention, during in-situ stratified culture, involves adjusting an electric telescopic rod to deflect a deflecting roller, thereby separating the sealing plate from the mixing ball, exposing the drainage holes on the outside. Seawater from the ocean is then poured into the mixing cylinder. Next, the electric telescopic rod resets the sealing plate, and a drive motor rotates the sealing plate 360°, removing impurities located at the drainage holes. The culture medium is then introduced into the mixing cylinders at each level, mixing the seawater with the culture medium. The mixed culture medium is then discharged from the drainage holes, which are evenly distributed throughout the mixing cylinders, ensuring that the culture medium is uniformly discharged to the designated locations.

Description

Technical Field

[0001] This invention relates to the field of carbon and nitrogen fixation technology in the ocean, and particularly to an in-situ stratified culture device and method for improving carbon and nitrogen fixation rates in the ocean. Background Technology

[0002] The process of converting free nitrogen in the air into combined nitrogen is called nitrogen fixation; carbon fixation, also known as carbon sequestration, refers to measures to increase the carbon content of the carbon pool outside the atmosphere, with the aim of reducing the concentration of carbon dioxide in the atmosphere; in order to improve the carbon and nitrogen fixation rate in the ocean, in-situ stratified cultivation is required.

[0003] In existing in-situ stratified culture devices, after the device filled with culture medium is introduced into the ocean and submerged to a designated depth, the culture medium is discharged. However, due to the irregular direction of water flow and the increase in water pressure, the discharged culture medium flows disorderly at the designated location and cannot be evenly distributed. As a result, the carbon-fixing and nitrogen-fixing organisms at that location cannot be effectively cultured, thus reducing the use value of the in-situ stratified culture device. Summary of the Invention

[0004] This invention discloses an in-situ stratified culture device and method for improving carbon and nitrogen fixation rates in the open ocean. It aims to solve the technical problem that in existing in-situ stratified culture devices, after the device filled with culture medium is introduced into the open ocean and submerged to a designated depth, the discharged culture medium flows disorderly at the designated location due to the irregular direction of water flow and increased water pressure, failing to disperse evenly and thus preventing the effective cultivation of carbon and nitrogen-fixing organisms at that location.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An in-situ stratified culture device for carbon and nitrogen fixation in the open ocean includes a feeding cylinder. Multiple sets of equalizing spheres are arranged on the outer side of the feeding cylinder, and each equalizing sphere includes a mixing cylinder. A connecting frame is fixedly connected to the side wall of the feeding cylinder near the mixing cylinder. A motor frame is fixedly connected to one side of the connecting frame, and a drive motor is fixedly connected to the side of the motor frame away from the feeding cylinder. The output shaft of the drive motor is fixedly connected to a drive shaft via a coupling. A rotating frame is fixedly connected to the end of the drive shaft. A docking hole is opened on the side wall of the feeding cylinder near the mixing cylinder, and a connecting pipe is fixedly connected inside the docking hole. A solenoid valve is connected to the outer side of the connecting pipe via a flange. One end of the connecting pipe is fixedly connected to the inside of the mixing cylinder. Drainage holes are evenly spaced on the outer side of the mixing cylinder.

[0007] By incorporating a uniformly distributed ball, during in-situ layered culture, the electric telescopic rod is adjusted to drive the deflection roller, causing the sealing plate to separate from the mixing ball. The drainage holes are exposed on the outside, allowing seawater from the ocean area to flow into the mixing cylinder. The electric telescopic rod then resets the sealing plate, and the drive motor rotates it 360°, removing impurities from the drainage holes. The culture medium is then introduced into the mixing cylinders at each layer, mixing the seawater with the culture medium. The mixed culture medium is then discharged through the drainage holes, which are evenly distributed throughout the mixing cylinder, ensuring uniform discharge of the culture medium to the designated locations, improving the culture effect, and ultimately increasing carbon and nitrogen fixation efficiency.

[0008] In a preferred embodiment, mounting blocks are arranged in a ring on the side of the rotating frame facing the mixing cylinder, and a shaft frame is fixedly connected to the outer side of each mounting block. A connecting shaft is connected to the inner walls on both sides of each shaft frame via bearings. A deflection roller is fixedly connected to the outer side of the connecting shaft, and a fitting sealing plate is fixedly connected to the outer side of the deflection roller. A fixing block is fixedly connected to the outer side of the rotating frame near each shaft frame. An electric telescopic rod is hinged to the side of the fixing block facing the shaft frame. A push block is hinged to the output end of the electric telescopic rod, and the push block is fixedly connected to the side wall of the deflection shaft.

[0009] In a preferred embodiment, scraper blades are hinged to both sides of the fitting sealing plate at equal distances, and mounting arc plates are fixedly connected to the outer side of the fitting sealing plate near the scraper blades at equal distances. Connecting rods are fixedly connected to both ends of the top of the mounting arc plates, and vibrating balls are fixedly connected to the ends of the connecting rods. A vibrating spring rod is fixedly connected to the side of the mounting arc plate facing the scraper blades, and one end of the vibrating spring rod is fixedly connected to the outer side of the scraper blades.

[0010] By incorporating a scraper, a vibrating ball, and a vibrating spring rod, the mixing cylinder addresses the potential for blockage caused by seawater carrying impurities when the drain hole is open. After introducing some seawater into the mixing cylinder, the drive motor rotates the scraper, which scrapes off the impurities adhering to the drain hole. Simultaneously, when the scraper contacts the outside of the mixing cylinder, the vibrating spring rod is compressed. As the mixed culture medium is discharged from the mixing cylinder, the vibrating spring rod gradually returns to its original position, thus working in conjunction with the vibrating ball to vibrate the scraper, quickly removing the impurities adhering to the scraper and ensuring optimal performance for the next use.

[0011] In a preferred embodiment, the mixing cylinder is provided with an internal auxiliary component, which includes an internal rotating ring. A sealing cap is fixedly connected to one end of the mixing cylinder near the motor frame, and the internal rotating ring is fixedly connected to the side of the sealing cap facing the inside of the mixing cylinder.

[0012] In a preferred embodiment, a second drive motor is fixedly connected to one side of the built-in rotating ring, and the output shaft of the second drive motor is fixedly connected to a second drive shaft via a coupling. Sleeves are fixedly connected to the outer sides of both ends of the second drive shaft. Telescopic connecting rods are distributed in a ring around the outer sides of both sleeves. The ends of two telescopic connecting rods located on the same horizontal plane on different sleeves are fixedly connected to the same assembly plate. A stirring tooth is fixedly connected to the outer side of each assembly plate. The same telescopic sealing strip is fixedly connected to the outer sides of every two adjacent assembly plates. End sealing plates are provided at the ends of multiple assembly plates and multiple telescopic sealing strips. An electric telescopic rod is distributed in a ring around the outer side of the second drive shaft, and the output end of the electric telescopic rod is fixedly connected to the outer side of an adjacent assembly plate.

[0013] With built-in auxiliary components, after the culture medium is mixed with seawater, it is introduced into the external seawater. The second drive motor is activated, which drives each stirring tooth to stir the mixture of culture medium and seawater inside the mixing cylinder, improving the uniformity of the mixture. At the same time, during the discharge process, the second electric telescopic rod is adjusted to drive the assembled rigid plate to gradually unfold outward, thereby squeezing the space inside the mixing cylinder and assisting the culture medium to be discharged from the drain hole to the corresponding position. The stirring teeth play a certain rotational driving role during the rotation process, thereby further improving the uniformity of the culture medium discharge.

[0014] In a preferred embodiment, an embedded column is fixedly connected to the bottom inner wall of the feeding cylinder, and a sealing cap is placed on the top of the embedded column. An upper bonding frame is fixedly connected at equal intervals to the outer side of the embedded column. Two electric telescopic rods are fixedly connected to the bottom of the upper bonding frame. The output ends of the two electric telescopic rods located on the same upper bonding frame are fixedly connected to the same lifting pressure plate, and the lifting pressure plate is in contact with the outer wall of the embedded column.

[0015] In a preferred embodiment, each embedded column has a discharge hole on the outer side near each connecting pipe, and a discharge pipe is fixedly connected inside each discharge hole. A solenoid valve is connected to the outer side of the discharge pipe via a flange.

[0016] In a preferred embodiment, a docking frame is fixedly connected to the outer wall of the feeding cylinder away from the mixing cylinder, and a lifting slide groove is opened on one side of the docking frame. A lifting slider is slidably connected inside the lifting slide groove. A support plate is fixedly connected to the side of the docking frame located below the lifting slide groove. An electric telescopic rod three is fixedly connected to the top of the support plate. The output end of the electric telescopic rod three is fixedly connected to the bottom of the lifting slider.

[0017] In a preferred embodiment, the top of the lifting slider has two positioning holes, and a guide rail is fixedly connected to the top of the lifting slider. Follower sliders are slidably connected inside the guide rail at both ends of the positioning holes. Locking rings are fixedly connected to the outer side of the follower sliders, and the locking rings are located outside the positioning holes. The same electric telescopic rod is fixedly connected to the opposite side of the two follower sliders located outside the same positioning hole. Mounting side frames are fixedly connected to both sides of the lifting slider, and cameras are fixedly connected to the side of the two mounting side frames facing the mixing ball.

[0018] An in-situ stratified culture method for improving carbon and nitrogen fixation rates in the open ocean, using the in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean as described above, the culture method comprising the following steps:

[0019] Step 1: The submersible device is connected to the feeding cylinder. Adjust the telescopic rod three to move the lifting slider to the designated position. Then, insert the two positioning rods in the submersible device into the positioning holes on the lifting slider. Adjust the electric telescopic rod four to move the follower slider in the guide rail. The two locking rings gradually move towards the positioning rods to quickly complete the clamping and fixing of the two positioning rods. After the connection is completed, the feeding cylinder is moved to the designated position by the submersible device so that each mixing cylinder is distributed on the corresponding layer.

[0020] Step 2: During in-situ stratified culture, adjust the electric telescopic rod one to drive the deflection roller to deflect, thereby separating the fitting sealing plate from the mixing ball, exposing the outlet hole on the outside, and the seawater inside the ocean area is poured into the mixing cylinder. Then, the electric telescopic rod one drives the fitting sealing plate to reset. After some seawater is poured into the mixing cylinder, open the solenoid valve one and solenoid valve two, and adjust the electric telescopic rod five to drive the lifting pressure plate to squeeze downward. The culture liquid introduced into the feeding cylinder is squeezed out through the lifting pressure plate and introduced into the mixing cylinder.

[0021] Step 3: After the culture medium and seawater are mixed, drive motor 2 is started. Drive motor 2 drives each stirring tooth to stir the mixture of culture medium and seawater inside the mixing cylinder, improving the uniformity of the mixture. At the same time, during the discharge process, electric telescopic rod 2 is adjusted to drive the assembled hard plate to gradually unfold outward, thereby squeezing the space inside the mixing cylinder and assisting the culture medium to be discharged from the drain hole into the corresponding position, completing the in-situ stratified culture.

[0022] As can be seen from the above, the in-situ layered culture device for carbon and nitrogen fixation in the ocean provided by the present invention, during in-situ layered culture, adjusts the electric telescopic rod to drive the deflection roller to deflect, thereby separating the fitting sealing plate from the mixing ball, exposing the drain hole to the outside, and the seawater inside the ocean is poured into the mixing cylinder. Then, the electric telescopic rod drives the fitting sealing plate to reset, and the drive motor is started to drive the fitting sealing plate to rotate 360°, removing impurities located at the drain hole. Then, the culture medium is introduced into the mixing cylinders of each layer, so that the seawater and the culture medium are mixed. Then, the mixed culture medium is discharged from the drain hole. The drain hole is evenly distributed in various positions of the mixing cylinder, thereby ensuring that the culture medium can be evenly discharged to the designated position, improving the culture effect, and thus improving the efficiency of carbon and nitrogen fixation. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of an in-situ stratified culture device for improving carbon and nitrogen fixation rates in the ocean area, as proposed in this invention.

[0024] Figure 2 This is a front view of the overall structure of an in-situ stratified culture device for improving carbon and nitrogen fixation rates in the ocean area, as proposed in this invention.

[0025] Figure 3 This is an enlarged view of the fitted closed sheet structure of an in-situ layered culture device for improving carbon and nitrogen fixation rates in the ocean area, as proposed in this invention.

[0026] Figure 4 This is a structural breakdown diagram of the fitting sealing sheet and mixing cylinder of an in-situ layered culture device for improving carbon and nitrogen fixation rates in the ocean area, as proposed in this invention.

[0027] Figure 5 This is a schematic diagram of the evenly distributed sphere structure of an in-situ stratified culture device for improving carbon and nitrogen fixation rates in the ocean area, as proposed in this invention.

[0028] Figure 6 for Figure 5 Enlarged view of the structure of part A in the middle.

[0029] Figure 7 This is a schematic diagram of the built-in auxiliary components of an in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean, as proposed in this invention.

[0030] Figure 8 This is a schematic diagram of the lifting slider and camera combination structure of an in-situ stratified culture device for improving carbon and nitrogen fixation rates in the ocean area, as proposed in this invention.

[0031] Figure 9 This is a cross-sectional view of the feeding cylinder and embedded column structure of an in-situ stratified culture device for carbon and nitrogen fixation rate in the ocean area proposed in this invention.

[0032] In the diagram: 1. Feeding cylinder; 2. Sealing cap; 3. Embedded column; 4. Connecting frame; 5. Distributing ball; 501. Fitting sealing plate; 502. Drive motor one; 503. Drive shaft one; 504. Rotating frame; 505. Mixing cylinder; 506. Drain hole; 507. Shaft frame; 508. Mounting block; 509. Deflection roller; 510. Pushing block; 511. Fixing block; 512. Electric telescopic rod one; 6. Motor frame; 7. Solenoid valve one; 8. Connecting pipe; 9. Connecting frame; 10. Lifting slider; 11. Vibrating spring rod; 12. Scraper; 13. Connecting thin rod; 14. Mounting arc plate; 15. Vibrating ball; 16. Sealing cap; 7. Built-in auxiliary components; 1701. Built-in rotating ring; 1702. Stirring teeth; 1703. Telescopic sealing strip; 1704. Assembly hard plate; 1705. Electric telescopic rod II; 1706. End sealing plate; 1707. Drive shaft II; 1708. Sleeve; 1709. Telescopic connecting rod; 1710. Drive motor II; 18. Support plate; 19. Electric telescopic rod III; 20. Mounting side frame; 21. Locking ring; 22. Camera; 23. Follow-up slider; 24. Electric telescopic rod IV; 25. Positioning hole; 26. Upper bonding frame; 27. Lifting pressure plate; 28. Discharge pipe; 29. ​​Solenoid valve II; 30. Electric telescopic rod V. Detailed Implementation

[0033] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0034] The present invention discloses an in-situ stratified culture device and method for improving carbon and nitrogen fixation rates in the ocean. This invention is mainly applied to situations where, during the use of existing in-situ stratified culture devices, after the device filled with culture medium is introduced into the ocean and submerged to a designated depth, the culture medium is discharged. However, due to the irregular direction of water flow and the increase in water pressure, the discharged culture medium flows disorderly at the designated location and cannot be evenly dispersed, resulting in the inability of the carbon and nitrogen fixing organisms at that location to be effectively cultured.

[0035] Reference Figures 1-9An in-situ stratified culture device for carbon and nitrogen fixation rate in the ocean includes a feeding cylinder 1. Multiple sets of equalizing spheres 5 are provided on the outer side of the feeding cylinder 1, and each equalizing sphere 5 includes a mixing cylinder 505. A connecting frame 4 is fixedly connected to the side wall of the feeding cylinder 1 near the mixing cylinder 505. A motor frame 6 is fixedly connected to one side of the connecting frame 4, and a drive motor 502 is fixedly connected to the side of the motor frame 6 away from the feeding cylinder 1. The output shaft of the drive motor 502 is fixedly connected to a drive shaft 503 via a coupling. A rotating frame 504 is fixedly connected to the end of the drive shaft 503. A docking hole is opened on the side wall of the feeding cylinder 1 near the mixing cylinder 505, and a connecting pipe 8 is fixedly connected inside the docking hole. A solenoid valve 7 is connected to the outer side of the connecting pipe 8 via a flange. One end of the connecting pipe 8 is fixedly connected to the inside of the mixing cylinder 505. Drain holes 506 are opened at equal intervals on the outer side of the mixing cylinder 505.

[0036] In specific application scenarios, during in-situ layered culture, the electric telescopic rod 512 is adjusted to drive the deflection roller 509 to deflect, thereby separating the sealing plate 501 from the mixing ball, exposing the outlet hole 506 on the outside. Seawater from the ocean area is poured into the mixing cylinder 505. Then, the electric telescopic rod 512 drives the sealing plate 501 to reset, and the drive motor 502 is started to drive the sealing plate 501 to rotate 360°, removing impurities located at the outlet hole 506. Subsequently, the culture medium is introduced into the mixing cylinders 505 at each layer, so that the seawater and the culture medium are mixed. The mixed culture medium is then discharged from the outlet hole 506. The outlet hole 506 is evenly distributed in various positions of the mixing cylinder 505, thereby ensuring that the culture medium can be evenly discharged to the designated position, improving the culture effect, and thus improving the carbon and nitrogen fixation efficiency.

[0037] Reference Figures 1-5 In a preferred embodiment, mounting blocks 508 are arranged in a ring on the side of the rotating frame 504 facing the mixing cylinder 505, and a shaft frame 507 is fixedly connected to the outer side of each mounting block 508. A connecting shaft is connected to the inner walls on both sides of each shaft frame 507 through bearings. A deflection roller 509 is fixedly connected to the outer side of the connecting shaft. A fitting sealing plate 501 is fixedly connected to the outer side of the deflection roller 509. A fixing block 511 is fixedly connected to the outer side of the rotating frame 504 near each shaft frame 507. An electric telescopic rod 512 is hinged to the side of the fixing block 511 facing the shaft frame 507. A push block 510 is hinged to the output end of the electric telescopic rod 512. The push block 510 is fixedly connected to the side wall of the deflection shaft.

[0038] Reference Figure 1 , Figure 2 , Figure 5 and Figure 6In a preferred embodiment, scraper blades 12 are hinged to both sides of the fitting sealing plate 501 at equal distances, and mounting arc plates 14 are fixedly connected to the outer side of the fitting sealing plate 501 near the scraper blades 12 at equal distances. Connecting thin rods 13 are fixedly connected to both ends of the top of the mounting arc plates 14, and vibrating balls 15 are fixedly connected to the ends of the connecting thin rods 13. Vibrating spring rods 11 are fixedly connected to the side of the mounting arc plates 14 facing the scraper blades 12, and one end of the vibrating spring rods 11 is fixedly connected to the outer side of the scraper blades 12.

[0039] Specifically, when the drain hole 506 is open, the seawater carrying some impurities may clog the drain hole 506. After some seawater is introduced into the mixing cylinder 505, the drive motor 502 drives the scraper 12 to rotate. The scraper 12 scrapes off the impurities attached to the drain hole 506. At the same time, when the scraper 12 contacts the outside of the mixing cylinder 505, the oscillating spring rod 11 is in a compressed state. When the mixed culture medium inside the mixing cylinder 505 is discharged, the oscillating spring rod 11 gradually returns to its original position, thereby cooperating with the oscillating ball 15 to drive the scraper 12 to oscillate, quickly removing the impurities attached to the scraper 12, ensuring the best effect when used next time.

[0040] Reference Figure 1 , Figure 2 , Figure 4 and Figure 7 In a preferred embodiment, the mixing cylinder 505 has an internal auxiliary component 17, which includes an internal rotating ring 1701. A sealing cover 16 is fixedly connected to one end of the mixing cylinder 505 near the motor frame 6. The internal rotating ring 1701 is fixedly connected to the side of the sealing cover 16 facing the inside of the mixing cylinder 505. A second drive motor 1710 is fixedly connected to one side of the internal rotating ring 1701. The output shaft of the second drive motor 1710 is fixedly connected to a second drive shaft 1707 via a coupling. Sleeves 1708 are fixedly connected to the outer sides of the second drive shaft 1707 near both ends. Telescopic connecting rods are distributed in a ring on the outer sides of both sleeves 1708. 1709, the ends of two telescopic connecting rods 1709 located on the same horizontal plane on different sleeves 1708 are fixedly connected to the same assembly hard plate 1704. Each assembly hard plate 1704 has a stirring tooth 1702 fixedly connected to its outer side. Each pair of adjacent assembly hard plates 1704 has a telescopic sealing strip 1703 fixedly connected to its outer side. The ends of multiple assembly hard plates 1704 and multiple telescopic sealing strips 1703 are provided with end sealing pieces 1706. Electric telescopic rods 1705 are distributed in a ring on the outer side of the second drive shaft 1707. The output end of the electric telescopic rods 1705 is fixedly connected to the outer side of the adjacent assembly hard plate 1704.

[0041] It should be noted that during the process of introducing the culture medium and seawater into the external seawater after mixing, the second drive motor 1710 is started. The second drive motor 1710 drives each stirring tooth 1702 to stir the mixture of culture medium and seawater inside the mixing cylinder 505, thereby improving the uniformity of the mixture. At the same time, during the discharge process, the second electric telescopic rod 1705 is adjusted to drive the assembled rigid plate 1704 to gradually unfold outward, thereby squeezing the space inside the mixing cylinder 505 and assisting the culture medium to be discharged from the discharge hole 506 into the corresponding position. The stirring tooth 1702 plays a certain rotational driving role during the rotation process, thereby further improving the uniformity of the culture medium discharge.

[0042] Reference Figure 1 , Figure 2 and Figure 9 In a preferred embodiment, an embedded column 3 is fixedly connected to the bottom inner wall of the feeding cylinder 1, and a sealing cap 2 is placed on the top of the embedded column 3. An upper bonding frame 26 is fixedly connected at equal intervals to the outer side of the embedded column 3. Two electric telescopic rods 30 are fixedly connected to the bottom of the upper bonding frame 26. The output ends of the two electric telescopic rods 30 located on the same upper bonding frame 26 are fixedly connected to the same lifting pressure plate 27. The lifting pressure plate 27 is in contact with the outer side wall of the embedded column 3. A discharge hole is opened on the outer side of the embedded column 3 near each connecting pipe 8, and a discharge pipe 28 is fixedly connected inside each discharge hole. A solenoid valve 29 is connected to the outer side of the discharge pipe 28 through a flange.

[0043] Specifically, when delivering culture medium at various levels, after the feeding cylinder 1 is lowered to the designated point, each solenoid valve 29 is opened, and the electric telescopic rod 30 is adjusted to drive the lifting pressure plate 27 to squeeze downwards. The culture medium introduced into the feeding cylinder 1 is then expelled through the lifting pressure plate 27, thereby realizing the export of the culture medium.

[0044] Reference Figure 1 , Figure 2 and Figure 8In a preferred embodiment, a docking frame 9 is fixedly connected to the outer wall of the feeding cylinder 1 away from the mixing cylinder 505, and a lifting groove is opened on one side of the docking frame 9. A lifting slider 10 is slidably connected inside the lifting groove. A support plate 18 is fixedly connected to the side of the docking frame 9 located below the lifting groove. An electric telescopic rod 19 is fixedly connected to the top of the support plate 18. The output end of the electric telescopic rod 19 is fixedly connected to the bottom of the lifting slider 10. Two positioning holes 25 are opened on the top of the lifting slider 10, and a guide rail is fixedly connected to the top of the lifting slider 10. Follower sliders 23 are slidably connected inside the guide rail located at both ends of the positioning holes 25. A locking ring 21 is fixedly connected to the outside of the follower slider 23. The locking ring 21 is located outside the positioning hole 25. The same electric telescopic rod 24 is fixedly connected to the opposite side of the two follower sliders 23 located outside the same positioning hole 25. Mounting side frames 20 are fixedly connected to both sides of the lifting slider 10. Cameras 22 are fixedly connected to the side of the two mounting side frames 20 facing the mixing ball.

[0045] Specifically, before the feeding cylinder 1 is lowered, it needs to be fixed to the lowering equipment. First, adjust the telescopic rod three to move the lifting slider 10 to the designated position. Then, insert the two positioning rods in the lowering equipment into the positioning holes 25 on the lifting slider 10. Adjust the electric telescopic rod four 24 to move the follower slider 23 in the guide rail. The two locking rings 21 gradually move towards the positioning rods to quickly complete the clamping and fixing of the two positioning rods, and complete the docking of the feeding cylinder 1 with the lowering equipment.

[0046] An in-situ stratified culture method for improving carbon and nitrogen fixation rates in the open ocean is disclosed, using the aforementioned in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean. The culture method includes the following steps:

[0047] Step 1: The submersible device is connected to the feeding cylinder 1. The telescopic rod 3 is adjusted to move the lifting slider 10 to the designated position. Then, the two positioning rods in the submersible device are inserted into the positioning holes 25 on the lifting slider 10. The electric telescopic rod 4 24 is adjusted to move the follower slider 23 in the guide rail. The two locking rings 21 move towards the positioning rods to quickly clamp and fix the two positioning rods. After the connection is completed, the feeding cylinder 1 is moved to the designated position by the submersible device so that each mixing cylinder 505 is distributed on the corresponding layers.

[0048] Step 2: During in-situ stratified culture, adjust the electric telescopic rod 512 to drive the deflection roller 509 to deflect, thereby separating the sealing plate 501 from the mixing ball, exposing the outlet hole 506 on the outside, and the seawater inside the ocean area is poured into the mixing cylinder 505. Then, the electric telescopic rod 512 drives the sealing plate 501 to reset. After some seawater is poured into the mixing cylinder 505, open the solenoid valve 7 and the solenoid valve 29, and adjust the electric telescopic rod 30 to drive the lifting pressure plate 27 to squeeze downward. The culture liquid introduced into the feeding cylinder 1 is squeezed out through the lifting pressure plate 27, and the culture liquid is introduced into the mixing cylinder 505.

[0049] Step 3: After the culture medium and seawater are mixed, drive motor 2 1710 is started. Drive motor 2 1710 drives each stirring tooth 1702 to stir the mixture of culture medium and seawater inside the mixing cylinder 505, improving the uniformity of the mixture. At the same time, during the discharge process, the electric telescopic rod 2 1705 is adjusted to drive the assembled rigid plate 1704 to gradually unfold outward, thereby squeezing the space inside the mixing cylinder 505, assisting the culture medium to be discharged from the discharge hole 506 into the corresponding position, completing the in-situ layered culture.

[0050] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. An in-situ stratified culture device for carbon and nitrogen fixation rates in the open ocean, comprising a feeding cylinder (1), characterized in that, The feeding cylinder (1) has multiple sets of equalizing balls (5) on its outer side, and each equalizing ball (5) includes a mixing cylinder (505). A connecting frame (4) is fixedly connected to the side wall of the feeding cylinder (1) near the mixing cylinder (505). A motor frame (6) is fixedly connected to one side of the connecting frame (4), and a drive motor (502) is fixedly connected to the side of the motor frame (6) away from the feeding cylinder (1). The output shaft of the drive motor (502) is fixedly connected to a drive shaft (503) through a coupling. A rotating frame (504) is fixedly connected to the end of the drive shaft (503). A docking hole is opened on the side wall of the feeding cylinder (1) near the mixing cylinder (505), and a connecting pipe (8) is fixedly connected inside the docking hole. A solenoid valve (7) is connected to the outside of the connecting pipe (8) through a flange. One end of the connecting pipe (8) is fixedly connected to the inside of the mixing cylinder (505). The outside of the mixing cylinder (505) is equidistant from each other. The mixing cylinder (505) is provided with an internal auxiliary component (17), which includes an internal rotating ring (1701). A sealing cover (16) is fixedly connected to one end of the mixing cylinder (505) near the motor frame (6). The internal rotating ring (1701) is fixedly connected to the side of the sealing cover (16) facing the inside of the mixing cylinder (505). An embedded column (3) is fixedly connected to the bottom inner wall of the feeding cylinder (1), and a sealing cover (2) is placed on the top of the embedded column (3). An upper bonding frame (26) is fixedly connected at equal distances to the outside of the embedded column (3). Two electric telescopic rods (30) are fixedly connected to the bottom of the upper bonding frame (26). The output ends of the two electric telescopic rods (30) located on the same upper bonding frame (26) are fixedly connected to the same lifting pressure plate (27). The lifting pressure plate (27) is in contact with the outer wall of the embedded column (3).

2. The in-situ stratified culture device for carbon and nitrogen fixation rate in the ocean area according to claim 1, characterized in that, The rotating frame (504) has mounting blocks (508) arranged in a ring on the side facing the mixing cylinder (505), and each mounting block (508) is fixedly connected to a shaft frame (507) on its outer side. The inner walls of both sides of each shaft frame (507) are connected to a connecting shaft through bearings. A deflection roller (509) is fixedly connected to the outer side of the connecting shaft. A fitting sealing plate (501) is fixedly connected to the outer side of the deflection roller (509). A fixing block (511) is fixedly connected to the outer side of the rotating frame (504) near each shaft frame (507). An electric telescopic rod (512) is connected to the side of the fixing block (511) facing the shaft frame (507) through a hinge. A push block (510) is connected to the output end of the electric telescopic rod (512) through a hinge. The push block (510) is fixedly connected to the side wall of the deflection shaft.

3. The in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean area according to claim 2, characterized in that, Both sides of the fitting sealing plate (501) are connected to scraper blades (12) at equal distances via hinges. The fitting sealing plate (501) is fixedly connected to mounting arc plates (14) at equal distances on the outer side of the scraper blades (12). Both ends of the top of the mounting arc plates (14) are fixedly connected to connecting rods (13). The ends of the connecting rods (13) are fixedly connected to vibrating balls (15). The side of the mounting arc plates (14) facing the scraper blades (12) is fixedly connected to a vibrating spring rod (11). One end of the vibrating spring rod (11) is fixedly connected to the outer side of the scraper blades (12).

4. The in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean area according to claim 3, characterized in that, One side of the built-in rotating ring (1701) is fixedly connected to a second drive motor (1710), and the output shaft of the second drive motor (1710) is fixedly connected to a second drive shaft (1707) via a coupling. Sleeves (1708) are fixedly connected to the outer sides of both ends of the second drive shaft (1707). Telescopic connecting rods (1709) are arranged in a ring around the outer sides of both sleeves (1708). The ends of the two telescopic connecting rods (1709) located on the same horizontal plane on different sleeves (1708) are fixedly connected to the same assembly rigid plate (170). 4) Each assembled hard plate (1704) is fixedly connected to a stirring tooth (1702) on its outer side. The same telescopic sealing strip (1703) is fixedly connected to the outer side of each pair of adjacent assembled hard plates (1704). End sealing pieces (1706) are provided at the ends of multiple assembled hard plates (1704) and multiple telescopic sealing strips (1703). Electric telescopic rods (1705) are distributed in a ring on the outer side of the drive shaft (1707). The output end of the electric telescopic rods (1705) is fixedly connected to the outer side of the adjacent assembled hard plate (1704).

5. The in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean area according to claim 4, characterized in that, The embedded column (3) has a discharge hole on the outside of each connecting pipe (8), and a discharge pipe (28) is fixedly connected inside each discharge hole. A solenoid valve (29) is connected to the outside of the discharge pipe (28) through a flange.

6. The in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean area according to claim 5, characterized in that, The feeding cylinder (1) is fixedly connected to the outer wall away from the mixing cylinder (505) with a docking frame (9), and a lifting slide groove is opened on one side of the docking frame (9). A lifting slider (10) is slidably connected inside the lifting slide groove. A support plate (18) is fixedly connected to the side of the docking frame (9) located below the lifting slide groove. An electric telescopic rod three (19) is fixedly connected to the top of the support plate (18). The output end of the electric telescopic rod three (19) is fixedly connected to the bottom of the lifting slider (10).

7. The in-situ stratified culture device for improving carbon and nitrogen fixation rates in the open ocean area according to claim 6, characterized in that, The top of the lifting slider (10) has two positioning holes (25), and the top of the lifting slider (10) is fixedly connected to a guide rail. The guide rail is slidably connected to the inside of the two ends of the positioning hole (25). The outer side of the follower slider (23) is fixedly connected to a locking ring (21). The locking ring (21) is located outside the positioning hole (25). The two follower sliders (23) located outside the same positioning hole (25) are fixedly connected to the same electric telescopic rod (24) on opposite sides. Both sides of the lifting slider (10) are fixedly connected to mounting side frames (20). Both mounting side frames (20) facing the mixing ball are fixedly connected to a camera (22).

8. A method for in-situ layered culture of carbon and nitrogen fixation rates in the open ocean, using the in-situ layered culture apparatus for carbon and nitrogen fixation rates in the open ocean as described in claim 7, characterized in that... The cultivation method includes the following steps: Step 1: The submersible device is connected to the feeding cylinder (1). The telescopic rod 3 is adjusted to move the lifting slider (10) to the designated position. Then, the two positioning rods in the submersible device are inserted into the positioning holes (25) on the lifting slider (10). The electric telescopic rod 4 (24) is adjusted to move the follower slider (23) in the guide rail. The two locking rings (21) move towards the positioning rods to quickly complete the clamping and fixing of the two positioning rods. After the connection is completed, the feeding cylinder (1) is moved to the designated position through the submersible device so that each mixing cylinder (505) is distributed on the corresponding layers. Step 2: During in-situ stratified culture, adjust the electric telescopic rod 1 (512) to drive the deflection roller (509) to deflect, thereby separating the fitting sealing plate (501) from the mixing ball, exposing the outlet hole (506) to the outside, and the seawater inside the ocean area is poured into the mixing cylinder (505). Then, the electric telescopic rod 1 (512) drives the fitting sealing plate (501) to reset. After some seawater is poured into the mixing cylinder (505), open the solenoid valve 1 (7) and the solenoid valve 2 (29), adjust the electric telescopic rod 5 (30) to drive the lifting pressure plate (27) to squeeze downwards, and the culture liquid introduced into the feeding cylinder (1) is squeezed out through the lifting pressure plate (27), and the culture liquid is introduced into the mixing cylinder (505). Step 3: After the culture medium and seawater are mixed, drive motor 2 (1710) is started. Drive motor 2 (1710) drives each stirring tooth (1702) to stir the mixture of culture medium and seawater inside the mixing cylinder (505) to improve the uniformity of the mixture. At the same time, during the discharge process, electric telescopic rod 2 (1705) is adjusted to drive the assembly hard plate (1704) to gradually unfold outward, thereby squeezing the space inside the mixing cylinder (505) and assisting the culture medium to be discharged from the drain hole (506) into the corresponding position to complete the in-situ layered culture.

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