Thrips trapping composition containing horseradish, preparation method and application thereof
By combining horseradish and thrips pheromone in a specific ratio, a thrips trapping composition enhanced with horseradish is prepared and used, which solves the problems of drug resistance and environmental impact in thrips control and achieves efficient and stable control effects.
Patent Information
- Application Number
- CN202411068748.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-06
- Publication Date
- 2025-09-19
- Estimated Expiration
- 2044-08-06
AI Technical Summary
Existing methods for thrips control have problems such as the development of pesticide resistance, environmental impact, insufficient monitoring and prediction, difficulty in implementing integrated control, and seasonal and regional differences. There is a lack of low-toxic, safe and efficient biological control methods.
Horseradish is used as a synergist in combination with thrips pheromones. By mixing them in a specific ratio, the trapping effect of the thrips pheromones is improved, a thrips trapping composition containing horseradish is prepared, and a specific carrier is used for trapping.
It significantly improves the trapping effect of thrips pheromones, solves the problem of drug resistance, prolongs the duration of drug efficacy, reduces the negative impact on the environment, and achieves efficient and stable prevention and control effects.
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Figure BDA0004980186950000101
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of agricultural pest control, and in particular to a thrips trapping composition containing horseradish, and a preparation method and application thereof. Background Art
[0002] Thrips are a group of tiny insects belonging to the order Thysanoptera. They are tiny, with adults measuring approximately 0.5 to 5 mm in length. They are primarily found in tropical and temperate regions. There are many species of thrips, but the thrips that currently cause significant economic damage to crops primarily belong to the Thripidae family, including the Thrips and Frankliniella genera. The most serious thrips species worldwide include Frankliniella occidentalis, Frankliniella intonsa, Thrips palmi, Thrips tobacco thrips, and Megalurrothrips usitatus. Thrips are slender and feed on plant sap, causing damage to crops and affecting growth and yield. Some thrips species can also transmit plant viruses, exacerbating damage to crops and are considered pests in agriculture, posing a particular threat to cash crops such as flowers, vegetables, and fruits. Commonly affected crops include cotton, beans, tomatoes, grapes, roses, etc.
[0003] The current state of thrips control combines a variety of strategies and technologies aimed at effectively controlling this ubiquitous agricultural pest, while taking into account environmental friendliness and sustainable development. These mainly include the following means: (1) Agricultural control: This approach emphasizes the prevention and reduction of thrips damage through good agricultural practices. This includes clearing weeds and crop residues in the field to eliminate overwintering insect sources; rationally arranging crop planting time and layout, implementing crop rotation and intercropping; and enhancing the crop's own insect resistance through irrigation and fertilization management; (2) Physical control: Taking advantage of the thrips' tendency to specific colors, such as blue and yellow, sticky insect traps are set up to trap and kill adult insects. This method is particularly effective in greenhouses and small-scale crops, reducing the use of chemical pesticides; (3) Chemical control: Although the use of traditional pesticides such as imidacloprid and acetamiprid is still ongoing, the use of new pesticides such as thiamethoxam is increasingly popular due to the problem of resistance. These agents have a longer lasting effect and can more effectively control thrips. When using pesticides, emphasis should be placed on scientific application, such as applying pesticides in the evening or early morning when thrips are active, and fully covering all possible hiding places, including the soil, to improve the control effect; (4) Biological control: Control the thrips population by introducing and protecting natural enemies, such as certain predatory mites, parasitic wasps, and other natural enemies. The use of biological pesticides such as bacterial preparations, fungal preparations, and botanical insecticides is also increasing to reduce dependence on chemical pesticides; (5) Integrated control: Given the limitations of a single control method, the comprehensive use of multiple control methods has become the mainstream trend. This includes timely monitoring of pest occurrence, combining agricultural, physical, chemical, and biological control methods, and flexibly adjusting control strategies according to specific circumstances to achieve the goal of both controlling pests and protecting the ecological environment.
[0004] Although existing thrips control measures are quite comprehensive, there are still some shortcomings and challenges, such as (1) Development of pesticide resistance: Long-term reliance on chemical pesticides, especially the frequent use of single ingredients, has led to the rapid development of pesticide resistance in thrips. This means that the once effective chemical pesticides gradually become ineffective, and new pesticides need to be continuously developed to replace them, which increases the cost and difficulty of control. (2) Environmental impact: Chemical control may have negative impacts on non-target organisms and ecosystems, including the reduction of beneficial insects and water pollution, affecting biodiversity. (3) Biological control efficiency: Although biological control is an environmentally friendly method, in actual application, its effectiveness is affected by many factors, such as the adaptability of natural enemies, release timing, climatic conditions, etc., and it is sometimes difficult to quickly and large-scale control of thrips populations. (4) Insufficient monitoring and prediction: Accurate pest monitoring and prediction systems have not yet been popularized in all agricultural production areas, resulting in control often lagging behind the occurrence of pests and missing the best control time. (5) Difficulty in implementing integrated control: Although the integrated control strategy is ideal, in actual operation, it requires a high level of technology, capital investment and farmers' acceptance. Small-scale farmers may find it difficult to implement effectively due to lack of resources and knowledge; (6) Seasonal and regional differences: The occurrence patterns and degree of damage of thrips vary in different regions and seasonal conditions, requiring targeted prevention and control strategies, but general prevention and control guidelines may not be fully applicable.
[0005] The existing technology for the prevention and control of thrips cannot meet the current demand for the prevention of diseases and pests. There is an urgent need for a less toxic, safer and more efficient biological control method to fill the gap in the existing technology. Summary of the Invention
[0006] A first aspect of the present application provides a use of horseradish as a synergist in improving the thrips trapping effect of thrips pheromone.
[0007] Horseradish, also known as mustardin or water-soluble allyl isothiocyanate, is a secondary metabolite extracted from cruciferous plants such as horseradish. It is a volatile, oily liquid containing an N=C=S reactive group, exhibiting a pungent, spicy odor and fumigant activity. In agriculture, horseradish is an environmentally friendly, botanical pesticide with broad-spectrum, highly effective killing of various pathogenic fungi, bacteria, viruses, nematodes, and small insect pests. It can be used for pest control of vegetables and stored goods, as well as for soil disinfection, making it particularly suitable for the production of organic, green, and pollution-free agricultural products. There are no reports in the prior art that horseradish has a direct control effect on thrips. Based on general understanding in the field, it is theoretically possible that horseradish could affect the thrips' habitat through fumigation or directly affect the pests. However, because horseradish is primarily used for soil treatment and stored-goods pest control, there are currently no published reports of its direct control effect on flying pests such as thrips.
[0008] In the prior art, the combination of nicotine and horseradish has a control effect on thrips. Although it shows obvious synergistic effects and good effects in controlling pests such as strawberry thrips, it also has defects, such as (1) ecological safety issues: although horseradish is derived from plants and is relatively environmentally friendly, nicotine has certain ecotoxicity and may have adverse effects on non-target organisms such as beneficial insects and bees, thereby affecting the ecological balance; (2) residue issues: as an active ingredient, nicotine residues in agricultural products and the environment may cause concerns about food safety and environmental pollution, and it is necessary to strictly control the dosage and comply with the withdrawal period; (3) drug resistance risk: long-term use of nicotine and horseradish can cause pests to develop drug resistance; (4) the sustained release time of the drug effect after the combination of the two is not enough to meet the long-term control needs.
[0009] In the present application, thrips pheromones include two categories. One is aggregation pheromones, such as the aggregation pheromones of flower thrips and western flower thrips, whose components include neryl (S)-2-methylbutanoate and (R)-lavandulyl acetate; the aggregation pheromone of common giant thrips includes trans, trans-farnesyl acetate (E,E-farnesyl acetate) and the like; the other category is artificially synthesized plant volatiles, such as methylisonicotinate, p-anisaldehyde, eugenol, linalool, and (-)-verbenone.
[0010] Specifically, in the present invention, the thrips aggregation pheromone contains the following substances in a molar ratio: (R)-lavender acetate and chondrosyl (S)-2-methylbutyrate, and the ratio of the two is 1:4 to 1:8.
[0011] The optional ratio of the aggregation pheromone components of western flower thrips is (R)-lavender acetate: choleryl (S)-2-methylbutyrate (500-10000ng) = 1:8.
[0012] The optional ratio of the flower thrips aggregation pheromone components is (R)-lavender acetate: choleryl (S)-2-methylbutyrate (1000-5000ng) = 1:4.
[0013] In addition, in the present application, the thrips pheromone can also be a plant volatile. In parts by mass, the thrips pheromone contains the following substances: methyl isonicotinate (3-60%), 2-phenylethanol (1-40%), and anisaldehyde (0.5-20%).
[0014] Specifically, the thrips pheromone may be: methyl isonicotinate (45%), 2-phenylethanol (37%), and anisaldehyde (18%).
[0015] The applicant unexpectedly discovered in experiments that horseradish can be used as a synergist for thrips pheromones. The simultaneous addition of thrips pheromones and horseradish can significantly enhance the thrips trapping effect of thrips pheromones. Specifically, in the present invention, the thrips pheromones can be one or more of the aforementioned aggregation pheromones and plant volatiles.
[0016] The second aspect of the present application provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition includes thrips pheromone and horseradish.
[0017] In some embodiments, the mass ratio of the thrips pheromone to horseradish is 1:(0.05-1.2).
[0018] In some embodiments, the mass ratio of the thrips pheromone to horseradish is 1:(0.2-1.15).
[0019] In some embodiments, the mass ratio of the thrips pheromone to horseradish is 1:(0.8-1.1).
[0020] In some embodiments, the mass ratio of the thrips pheromone to horseradish is 1:1.
[0021] Horseradish enhances the trapping effect of thrips pheromones on thrips, but the same effect cannot be achieved by compounding them in any ratio. After a large number of field experiments, the applicant found that only when the mass ratio of thrips pheromones to horseradish is 1: (0.05-1.2), especially 1: 1, can the two exert their maximum trapping effect through their synergistic effect. Below or above this ratio, the trapping effect will be significantly reduced, and the stability of the efficacy will also be weakened accordingly. This may be because the addition of horseradish can penetrate the cell membrane of the pest, resulting in increased membrane permeability, disrupting the balance of ions inside and outside the cell, affecting cell function, further inhibiting the antioxidant defense system in the pest body, reducing the pest's resistance to oxidative stress, and accelerating the decline of its physiological function. In addition, the synergistic effect of the two can also enhance the repellent or antifeedant effect of the composition, and reduce its harm to crops by changing the behavior pattern of the pest.
[0022] The third aspect of the present application provides a method for preparing a thrips trapping composition containing horseradish, the preparation method comprising: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a carrier; the stabilizer comprises at least one of tert-butylhydroquinone, 3,4,5-trihydroxybenzoic acid n-propyl ester, 2,6-di-tert-butyl-4-methylphenol, and butylated hydroxyanisole.
[0023] In some embodiments, the content of the thrips trapping composition in each of the carriers is 0.01-0.5 g.
[0024] The carrier includes but is not limited to natural devulcanized rubber stoppers, capillary microtubes (such as slender and transparent PVC plastic tubes), polyethylene small black bottles or electrospun fiber material belts.
[0025] In some embodiments, the stabilizer includes at least one of tert-butylhydroquinone, 3,4.5-trihydroxybenzoic acid n-propyl ester, 2,6-di-tert-butyl-4-methylphenol, and butylated hydroxyanisole.
[0026] In some embodiments, the organic solvent includes at least one of olive oil, triglyceride, dioctyl terephthalate, chlorinated paraffin, and n-hexane.
[0027] The fourth aspect of the present application provides the use of the above-mentioned thrips trapping composition in thrips control.
[0028] A fifth aspect of the present application provides a method for controlling thrips, comprising: placing a thrips trapping composition with a carrier in a control area for trapping.
[0029] Compared with the prior art, the present invention has at least the following beneficial effects:
[0030] 1. The present application discovered that horseradish can be used as a synergist for thrips control. The simultaneous addition of horseradish and thrips pheromone can significantly improve the trapping effect of thrips pheromone on thrips.
[0031] 2. The present application has found that only when the mass ratio of thrips pheromone and horseradish is 1: (0.05-1.2), especially 1: 1, can the two exert the maximum trapping effect through their synergistic effect. If the ratio is lower or higher than this, the trapping effect will be significantly reduced and the stability of the efficacy will be weakened accordingly.
[0032] 3. The composition prepared in this application can solve the problem of drug resistance in the prior art for thrips control. It is a new control product with good stability, long action time and good application prospects.
[0033] Regarding the above-mentioned beneficial technical effects, the applicant needs to point out and emphasize that: although horseradish root may have a certain insecticidal effect when used alone, the applicant unexpectedly found that by adding thrips pheromone and horseradish root at the same time, this specific combination achieved a technical effect that neither of the two could achieve alone, which was completely unexpected by those skilled in the art; in addition, the applicant found through research that the ratio of thrips pheromone and horseradish root is very critical. Too little thrips pheromone results in insufficient trapping ability of the thrips trapping composition, while too much thrips pheromone results in insufficient horseradish root and thus the enhancing effect of horseradish root cannot be fully reflected; further, the applicant pointed out that the trapping effect (number of trapped, number of heads) and the duration of the trapping effect of the thrips trapping composition are It is very difficult to take both the intensity and duration of the trapping effect of the thrips trapping composition into account. If a very strong trapping effect is to be achieved, the amount of thrips pheromone needs to be relatively large, and accordingly, the amount of other components such as horseradish and stabilizers is relatively small, which will affect the action time of the composition, and it will not be possible to achieve a very long effect, and the sustained action time is limited. The applicant needs to emphasize again that it is very difficult to take both the intensity and duration of the trapping effect of the thrips trapping composition into account. On the one hand, it can achieve a very strong trapping effect, and on the other hand, it must maintain a long action time. However, the present application has achieved very good results in both trapping effect and action time by introducing horseradish into the thrips pheromone and conducting extensive research on the ratio of the two. DETAILED DESCRIPTION
[0034] To make the purpose, technical solutions and advantages of this application more clear, this application is further described in detail below. However, it should be understood that the description herein is only used to explain this application and is not intended to limit the scope of this application.
[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are intended solely for the purpose of describing specific embodiments and are not intended to limit this application. The reagents and instruments used herein are commercially available, and the characterization methods involved can be found in the relevant descriptions in the prior art and will not be further elaborated herein.
[0036] The thrips pheromone described in the present invention refers to plant volatiles, and the plant volatiles specifically include the following substances by mass: methyl isonicotinate (45%), 2-phenylethanol (37%), and anisaldehyde (18%).
[0037] Example 1
[0038] This embodiment provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition includes thrips pheromone and horseradish in a mass ratio of 1:0.8.
[0039] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0040] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0041] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0042] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0043] Example 2
[0044] This embodiment provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition includes thrips pheromone and horseradish in a mass ratio of 1:0.9.
[0045] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0046] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0047] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0048] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0049] Example 3
[0050] This embodiment provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition includes thrips pheromone and horseradish in a mass ratio of 1:1.
[0051] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0052] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is thirty times the total weight of the thrips pheromone, horseradish and the stabilizer.
[0053] The organic solvent is triglyceride, and the added amount of the organic solvent is 30% of the total weight of the thrips pheromone and horseradish.
[0054] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0055] Example 4
[0056] This embodiment provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition includes thrips pheromone and horseradish in a mass ratio of 1:1.1.
[0057] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0058] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is thirty times the total weight of the thrips pheromone, horseradish and the stabilizer.
[0059] The organic solvent is triglyceride, and the added amount of the organic solvent is 30% of the total weight of the thrips pheromone and horseradish.
[0060] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0061] Example 5
[0062] This embodiment provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition includes thrips pheromone and horseradish in a mass ratio of 1:1.2.
[0063] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0064] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0065] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0066] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0067] Comparative Example 1
[0068] This comparative example provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition comprises thrips pheromone and horseradish in a mass ratio of 1:0.4.
[0069] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0070] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0071] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0072] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0073] Comparative Example 2
[0074] This comparative example provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition comprises thrips pheromone and horseradish in a mass ratio of 1:0.5.
[0075] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0076] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0077] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0078] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0079] Comparative Example 3
[0080] This comparative example provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition comprises thrips pheromone and horseradish in a mass ratio of 1:0.6.
[0081] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0082] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0083] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0084] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0085] Comparative Example 4
[0086] This comparative example provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition comprises thrips pheromone and horseradish in a mass ratio of 1:0.7.
[0087] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0088] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0089] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0090] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0091] Comparative Example 5
[0092] This comparative example provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition comprises thrips pheromone and horseradish in a mass ratio of 1:1.3.
[0093] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0094] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0095] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0096] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0097] Comparative Example 6
[0098] This comparative example provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition comprises thrips pheromone and horseradish in a mass ratio of 1:1.4.
[0099] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0100] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0101] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0102] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0103] Comparative Example 7
[0104] This comparative example provides a thrips trapping composition containing horseradish, wherein the thrips trapping composition comprises thrips pheromone and horseradish in a mass ratio of 1:1.5.
[0105] The preparation method of the thrips trapping composition comprises the following steps: uniformly mixing the thrips trapping composition and a stabilizer in an organic solvent, and then putting the mixture into a natural desulfurized rubber plug to obtain the composition.
[0106] The stabilizer is tert-butylhydroquinone, and the added amount of the stabilizer is 3% of the total weight of the thrips pheromone and horseradish.
[0107] The organic solvent is triglyceride, and the added amount of the organic solvent is thirty times the total weight of the thrips pheromone, horseradish and stabilizer.
[0108] The content of the thrips trapping composition in each natural devulcanized rubber plug is 0.01 g.
[0109] Comparative Example 8
[0110] In this comparative example, only horseradish is used as the trapping agent, and the content of horseradish in each natural devulcanized rubber plug is 0.01 g.
[0111] Comparative Example 9
[0112] In this comparative example, only thrips pheromone was used as the trapping agent, and the content of thrips pheromone in each natural desulfurized rubber plug was 0.01 g.
[0113] The natural desulfurized rubber plugs prepared in the above embodiments and comparative examples were used in conjunction with triangular traps, with each trap equipped with a natural desulfurized rubber plug. The experiment was carried out in the rainbow bean field of Lingao Village, Yazhou District, Sanya City, Hainan Province. On the afternoon of March 29, 2024, thrips attractants were set in the 2-mu red bean field, with 2 natural desulfurized rubber plugs per mu. The number of thrips trapped on the 7th, 14th, 21st and 35th days was recorded respectively. After each stage, the dead insects in the trap were cleaned in time, and the results were recorded in Table 1.
[0114] Table 1
[0115]
[0116] The above performance test results show that Examples 1-5 have good trapping effects, especially Example 3, which has the most outstanding overall performance, the strongest trapping ability, and the strongest stability. This is mainly because horseradish can be used as a synergist. The simultaneous addition of the two can significantly improve the trapping effect of thrips pheromone on thrips. In addition, when the mass ratio of thrips pheromone to horseradish is 1:1, good trapping effect can be achieved, and a longer duration can be achieved. In Comparative Examples 1-7, the mass ratio of thrips pheromone to horseradish is changed, and it can be seen that the trapping effect decreases, demonstrating that the ratio specified in this application has a significant impact on both the trapping effect and the stability of the composition.
[0117] In addition, in Comparative Example 8, only horseradish was used as a trapping agent, which failed to achieve a good trapping effect and had poor sustainability. Furthermore, compared with Comparative Example 9 in which only thrips pheromone was used, it was demonstrated that the addition of horseradish can improve the stability of thrips pheromone in the environment and prolong the duration of action.
[0118] The applicant declares that while the present application illustrates the solutions of the present application through the above-mentioned embodiments, the present application is not limited to the above-mentioned embodiments, which does not mean that the present application must rely on the above-mentioned embodiments in order to be implemented. Those skilled in the art should understand that any improvements to the present application, equivalent replacements of the raw materials of the present application's products, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present application.
[0119] The preferred embodiments of the present application are described in detail above. However, the present application is not limited to the specific details of the above embodiments. Within the technical concept of the present application, various simple modifications can be made to the technical solution of the present application, and these simple modifications all fall within the scope of protection of the present application.
[0120] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner unless there is any contradiction. In order to avoid unnecessary repetition, this application will not further describe various possible combinations.
Claims
1. A thrips trapping composition, characterized in that: The thrips trapping composition comprises thrips pheromone and horseradish, The thrips pheromone refers to plant volatiles, which specifically include the following substances by mass: methyl isonicotinate (45%), 2-phenylethanol (37%), and anisaldehyde (18%); The mass ratio of the thrips pheromone to horseradish is 1: (0.8-1.1); The thrips trapping composition further comprises an organic solvent and a stabilizer, The stabilizer includes at least one of tert-butylhydroquinone, 3,4,5-trihydroxybenzoic acid n-propyl ester, 2,6-di-tert-butyl-4-methylphenol, and butylated hydroxyanisole.
2. The thrips trapping composition according to claim 1, characterized in that The mass ratio of the thrips pheromone to horseradish is 1:
1.
3. A method for preparing the thrips trapping composition according to any one of claims 1 to 2, characterized in that: The preparation method comprises: uniformly mixing a thrips trapping composition and a stabilizer in an organic solvent, and then placing the mixture into a carrier; the stabilizer comprises at least one of tert-butylhydroquinone, 3,4,5-trihydroxybenzoic acid n-propyl ester, 2,6-di-tert-butyl-4-methylphenol, and butylated hydroxyanisole.
4. The preparation method according to claim 3, characterized in that The content of the thrips trapping composition in each carrier is 0.01-0.5g.
5. Use of the thrips trapping composition according to any one of claims 1 to 2 in thrips control.
6. A method for controlling thrips using the thrips trapping composition according to any one of claims 1 to 2, characterized in that: The method comprises: placing a thrips trapping composition with a carrier in a prevention and control area for trapping.
7. Use of the thrips trapping composition according to any one of claims 1 to 2 in improving the thrips trapping effect of thrips pheromones.
Citation Information
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