A high uniformity slide staining system

Abstract

The present invention relates to a high-uniformity slide staining system applied to the field of slide staining technology, comprising a system track, a slide module, a staining module, a staining positioning detection module, a dyeing dripping component, a cleaning component, a slide recording unit and a dyeing position recording unit. The present invention allows the slide to be normally stained by a No. 1 staining module first, and then when the No. 2 staining module is about to be stained, the dyeing positioning detection module detects the staining position of the slide, the dyeing position recording unit corresponds the staining position to the slide data of the slide recording unit, and provides accurate staining position for the No. 2 staining module and subsequent staining modules, and the dyeing dripping component accurately drips dye to the colored tissue, so as to realize uniform staining of the slide with a small amount of dye, effectively improve the dyeing uniformity of the slide, effectively reduce the waste of dye, and thus reduce the production cost of the slide.

Description

Technical Field

[0001] The invention relates to a glass slide staining system, in particular to a high-uniformity glass slide staining system applied in the technical field of glass slide staining. Background Art

[0002] A slide is a piece of glass or quartz used to place objects when observing with a microscope. When making samples, cells or tissue slices are placed on the slide, and a cover glass is placed on it for observation. In optics, a thin piece of glass-like material is used to produce phase difference. The sample needs to be stained during the production process of the slide, and the staining of the sample is a key factor in the successful production of the slide. Traditionally, the staining of slides is done manually, and the method of putting the slide into a staining container for staining is time-consuming and labor-intensive. The staining process lacks unified operating specifications and standards, and the efficiency of slide production is low.

[0003] The specification of Chinese invention patent CN201811614966.9 discloses "A system for automatic staining of glass slides". The invention uses a sampling gripper to grab the staining component at the outlet end of the input conveying mechanism and send it to the transport component on the conveying chain. At each staining station, the sample loading mechanism injects dye into the staining component for staining or cleaning. After the predetermined time is reached, the rotating hand of the flipping robot and the transport component act together on the staining component, and the rotating hand is driven by a rotating motor to flip 180°, pouring the stain or cleaning liquid into the waste liquid pool below. After the staining is completed, it is transferred by the sampling gripper to the synchronous belt of the load-out conveying mechanism, realizing the assembly line automatic staining of the glass slide.

[0004] For sample tissues that need to be stained multiple times, the slides need to undergo multiple staining processes to highlight the hierarchy of the sample tissue and distinguish different types of tissue structures. In order to fully stain the samples on the slides, a large amount of dye needs to be used on the surface of the slides each time the staining is performed. The slides are directly infiltrated with a large amount of dye. This method of slide staining is prone to waste of dye. If the amount of dye used is reduced, it is easy to cause uneven staining of the slides, affecting the production quality of the slides. Summary of the invention

[0005] In view of the above-mentioned prior art, the technical problem to be solved by the present invention is that a large amount of dye is used on the surface of the slide so that the tissue sample is fully infiltrated with the dye, which easily causes a waste of the dye. However, a reduced amount of dye is likely to cause uneven dyeing of the slide, affecting the production quality of the slide.

[0006] To solve the above problems, the present invention provides a high-uniformity slide staining system, including a system track, wherein the movable end of the system track is fixedly connected with equidistantly arranged slide modules, the middle of the slide module is fixedly connected with a slide, and the top of the system track is fixedly connected with equidistantly arranged staining modules, and the staining modules correspond vertically to the slide modules; the front and rear ends of the system track are respectively fixedly connected with an input table and an output table, both of which correspond to the slide modules, and a plurality of staining modules are equidistantly arranged from the input table to the output table, and the middle of the second staining module is fixedly connected with a staining positioning detection module, and the two ends of the staining module are respectively fixedly connected with a drip dye component and a cleaning component, and the drip dye component is close to the input table, and the drip dye component is fixedly connected to the detection end of the staining positioning detection module; the output ends of the input table and the output table are commonly connected with a slide recording unit, the input end of the slide recording unit is connected with a staining position recording unit, the input end of the staining position recording unit is connected to the staining positioning detection module, and the output end of the slide recording unit is connected to the control end of the drip dye component.

[0007] In the above-mentioned high-uniformity slide staining system, the slide with the sample tissue is driven to move on the system track by the slide module. After the slide is stained by the No. 1 staining module and is about to be stained by the No. 2 staining module, the staining positioning detection module detects the staining position of the slide, and provides accurate staining position for the No. 2 staining module and subsequent staining modules, thereby achieving uniform staining of the slide with a small amount of stain, effectively improving the staining uniformity of the slide, and effectively reducing the waste of stain, thereby reducing the production cost of the slide.

[0008] As a further improvement of the present application, the dye drip assembly is made of a T-shaped electric rail, and the movable end of the T-shaped electric rail is fixedly connected to the dye barrel, the dye positioning detection module is fixed to the outside of the dye barrel, and the T-shaped electric rail can flexibly adjust the position of the dye barrel to facilitate accurate dripping of the dye on the glass slide.

[0009] As a further improvement of the present application, the slide module includes an end block, which is fixedly connected to the movable end of the system track. The middle part of the end block is rotatably connected to a rotating clamp, which clamps and fixes the glass slide. The glass slide is clamped and fixed by the rotating clamp, which facilitates the transmission and movement of the glass slide on the system track.

[0010] As a further improvement of the present application, the rotating clamp is arranged in an L-shape and vertically bent, and a chuck is hinged at the top of the short end of the rotating clamp, the lower surface of the chuck contacts the surface of the glass slide, and the chuck presses one end of the glass slide, effectively improving the clamping stability of the glass slide.

[0011] As another improvement of the present application, a water inlet is fixedly connected to the middle part of the chuck, and the water outlet end of the cleaning component corresponds to the water inlet. A water seepage groove is provided on the lower surface of the chuck, and the water seepage groove is connected to the water inlet. The cleaning component inputs cleaning water into the water inlet, and the water then seeps out evenly from the water seepage groove, which makes it convenient for the cleaning water to slowly rinse the surface of the glass slide, thereby effectively improving the cleaning effect of the glass slide.

[0012] As another improved supplement to the present application, a rotating wheel is fixedly connected to one end of the rotating clamp close to the end block, and tooth pads are staggered and fixedly connected to the top and bottom of the system track. The rotating wheel is engaged with the tooth pads, and the rotating wheel contacts the tooth pads, driving the rotating clamp to rotate, so that the glass slide on the rotating clamp is in an inclined state, which is convenient for cleaning the glass slide.

[0013] As another improved supplement of the present application, the tooth groove length of the tooth pad is one eighth of the circumference of the outer ring of the rotating wheel, the surface of the end block is fixedly connected with a fan-shaped groove, the surface of the rotating wheel is fixedly connected with a positioning block, the positioning block is slidably connected to the fan-shaped groove, and the movable angle of the positioning block is 45°, so that the rotating clamp can drive the glass slide to rotate between horizontal and 45°, which is convenient for the staining and cleaning operations of the glass slide.

[0014] As another improvement of the present application, adsorption blocks are fixedly embedded at both ends of the fan-shaped groove, and the adsorption blocks are adsorbed correspondingly to the positioning blocks. The positioning blocks and the adsorption blocks are both made of magnetic materials. The positioning blocks and the adsorption blocks attract each other to achieve the fixation of the inclined state and the horizontal state of the glass slide, thereby effectively improving the stability of the glass slide during staining and cleaning.

[0015] As another improvement supplement of the present application, the long end surface of the rotating clamp is slidably connected with a glass slide pad, the glass slide pad corresponds to the edge of the glass slide, and a spring is fixedly connected between the glass slide pad and the curved portion of the rotating clamp. When the glass slide is horizontally pushed into the rotating clamp, the glass slide pad limits the position of the glass slide, thereby facilitating precise clamping of the glass slide.

[0016] As another improved supplement of the present application, an output push rod is fixedly connected to the middle part of the output table, and a slide hook is connected to the output end of the output push rod. The slide hook is parallel to the glass slide pad, and the top of the slide hook corresponds to the edge of the glass slide. When the rotating clamp moves to the output table position, the slide hook pushes away and replaces the glass slide pad, and the output push rod subsequently pulls the slide hook to realize the automatic removal of the glass slide.

[0017] In summary, the present invention places a glass slide on a slide carrier module to realize the transmission movement of the glass slide on the system track. After the glass slide is normally stained by the No. 1 staining module and is about to be stained by the No. 2 staining module, the staining positioning detection module detects the staining position of the glass slide, and the staining position recording unit records the slide data of the glass slide corresponding to the staining position, and provides accurate staining position information for the No. 2 staining module and subsequent staining modules. The dye dripping component accurately drips the dye to the already colored tissue, thereby realizing uniform staining of the glass slide with a small amount of dye, effectively improving the dyeing uniformity of the glass slide, and effectively reducing the waste of dye, thereby reducing the production cost of the glass slide. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a three-dimensional structural diagram of the first embodiment of the present application;

[0019] Figure 2 This is a three-dimensional structural diagram of a dyeing module according to the first embodiment of the present application;

[0020] Figure 3 This is a three-dimensional structural diagram of the dyeing module and the dyeing positioning detection module of the first embodiment of the present application;

[0021] Figure 4 This is a system diagram of the first implementation mode of the present application;

[0022] Figure 5 This is a demonstration diagram of the rotation change of the rotating clamp in the second embodiment of the present application;

[0023] Figure 6 This is a three-dimensional structural diagram of a cleaning assembly and a rotating clamp according to a second embodiment of the present application;

[0024] Figure 7 This is a three-dimensional structural diagram of a rotary clamp according to a second embodiment of the present application;

[0025] Figure 8 This is a three-dimensional structural diagram of the rotor and the end block of the second embodiment of the present application;

[0026] Fig. 9 This is an enlarged view of the output station of the second embodiment of the present application;

[0027] Fig.10 This is a side cross-sectional view of the output station according to the second embodiment of the present application.

[0028] Description of the numbers in the figure:

[0029] 1. System track; 101. Input platform; 102. Output platform; 2. Slide carrier module; 201. End block; 202. Rotating clamp; 203. Chuck; 204. Water inlet; 205. Water seepage trough; 3. Staining module; 301. Dye drip assembly; 302. Cleaning assembly; 303. T-type electric rail; 304. Dye cylinder; 4. Staining positioning detection module; 5. Rotating wheel; 501. Tooth pad; 502. Fan-shaped groove; 503. Positioning block; 504. Adsorption block; 6. Slide pad; 601. Spring; 602. Output push rod; 603. Slide hook; 7. Slide. DETAILED DESCRIPTION

[0030] Two implementation modes of the present application are described in detail below with reference to the accompanying drawings.

[0031] The first implementation method:

[0032] Figure 1 As shown, a high uniformity slide staining system comprises a system track 1, the movable end of the system track 1 is fixedly connected with slide modules 2 arranged at equal intervals, the middle of the slide module 2 is fixedly connected with a glass slide 7, and the top of the system track 1 is fixedly connected with staining modules 3 arranged at equal intervals, and the staining modules 3 correspond vertically to the slide modules 2;

[0033] The glass slide 7 is placed on the slide module 2 to realize the transmission movement of the glass slide 7 on the system track 1. Each staining module 3 continuously stains the glass slide 7 multiple times, which effectively improves the staining automation of the glass slide 7, thereby improving the production efficiency of the glass slide 7.

[0034] Figure 1-4 As shown, the front and rear ends of the system track 1 are respectively fixedly connected with an input platform 101 and an output platform 102, both of which correspond to the slide module 2, and a plurality of dyeing modules 3 are arranged equidistantly from the input platform 101 to the output platform 102, and a dyeing positioning detection module 4 is fixedly connected to the middle of the second dyeing module 3 in sequence, and the two ends of the dyeing module 3 are respectively fixedly connected with a drip dye component 301 and a cleaning component 302, and the drip dye component 301 is close to the input platform 101, and the drip dye component 301 is made of a T-shaped electric rail 303, and the movable end of the T-shaped electric rail 303 is fixedly connected to the cleaning component 302. A dye barrel 304 is connected, and the dyeing position detection module 4 is fixed to the outside of the dye barrel 304. The T-shaped electric rail 303 can flexibly adjust the position of the dye barrel 304, so that the dye can be accurately dripped on the glass slide 7. The dye dripping component 301 is fixedly connected to the detection end of the dyeing position detection module 4; the output ends of the input platform 101 and the output platform 102 are commonly connected to a glass slide recording unit, and the input end of the glass slide recording unit is connected to a dyeing position recording unit. The input end of the dyeing position recording unit is connected to the dyeing position detection module 4, and the output end of the glass slide recording unit is connected to the control end of the dye dripping component 301;

[0035] When the glass slide 7 is normally stained by the No. 1 staining module 3, the staining method is consistent with the traditional staining method, and the amount of dye used is the same. The purpose of this staining is mainly to highlight the outline of the sample tissue on the glass slide 7, that is, the overall staining of the sample tissue. The subsequent staining is to stain the details of the sample tissue. When the No. 2 staining module 3 is about to be stained, the staining positioning detection module 4 detects the staining position of the glass slide 7, and the staining position recording unit records the glass slide 7 data of the glass slide corresponding to the staining position, and provides the No. 2 staining module 3 and the subsequent staining modules 3 with accurate staining position. The dripping component 301 accurately drips the dye on the already colored tissue, and the accurately dripped dye accurately dyes the sample tissue, that is, the dye is dripped at the center of the original staining position, and the dye diffuses evenly outward, effectively improving the uniformity of dyeing, realizing uniform staining of the glass slide 7 with a small amount of dye, and effectively improving the uniformity of staining of the glass slide 7, effectively reducing the waste of dye, and thus reducing the production cost of the glass slide 7.

[0036] The second implementation method:

[0037] Compared with the first embodiment, this embodiment mainly adds a rotating clamp 202. The specific newly added structure is as follows, and the remaining structures are consistent with the first embodiment.

[0038] Figure 5-7 As shown, the slide module 2 includes an end block 201, which is fixedly connected to the movable end of the system track 1, and a rotating clamp 202 is rotatably connected to the middle of the end block 201, and the rotating clamp 202 clamps and fixes the glass slide 7. The glass slide 7 is clamped and fixed by the rotating clamp 202, which is convenient for the glass slide 7 to be transported and moved on the system track 1. The rotating clamp 202 is vertically bent in an L shape, and a clamp 203 is hinged at the top of the short end of the rotating clamp 202, and the lower surface of the clamp 203 contacts the surface of the glass slide 7. The clamp 203 One end of the glass slide 7 is pressed tightly, which effectively improves the clamping and stabilizing effect of the glass slide 7. A water inlet 204 is fixedly connected to the middle of the chuck 203. The water outlet end of the cleaning component 302 faces the water inlet 204. A water seepage groove 205 is provided on the lower surface of the chuck 203. The water seepage groove 205 is connected to the water inlet 204. The cleaning component 302 inputs cleaning water to the water inlet 204, and the water then seeps out evenly from the water seepage groove 205, which is convenient for the cleaning water to slowly rinse the surface of the glass slide 7, effectively improving the cleaning effect of the glass slide 7.

[0039] The glass slide 7 with the sample tissue is placed on the rotating clamp 202, and the clamp 203 presses and fixes one end of the glass slide 7 to achieve stable installation of the glass slide 7 and facilitate stable transmission of the glass slide 7. After the glass slide 7 is infiltrated with the stain, when the cleaning component 302 cleans the glass slide 7, the cleaning water output by the cleaning component 302 is poured into the water injection port 204, and the cleaning water then seeps out evenly from the seepage groove 205, so that the cleaning water evenly and slowly rinses the surface of the glass slide 7, effectively improving the cleaning effect of the glass slide 7, and effectively preventing the tissue sample from falling off due to scouring.

[0040] Figure 6-8 As shown, the end of the rotating clamp 202 close to the end block 201 is fixedly connected with a rotating wheel 5, the top and bottom of the system track 1 are staggered and fixedly connected with tooth pads 501, the rotating wheel 5 is meshed with the tooth pads 501, the rotating wheel 5 contacts the tooth pads 501, and drives the rotating clamp 202 to rotate, so that the glass slide 7 on the rotating clamp 202 is in an inclined state, which is convenient for the cleaning operation of the glass slide 7, the tooth groove length of the tooth pad 501 is one eighth of the circumference of the outer ring of the rotating wheel 5, the surface of the end block 201 is fixedly connected with a fan-shaped groove 502, the surface of the rotating wheel 5 is fixedly connected with a positioning block 503, and the positioning block 50 3 is slidably connected to the fan-shaped groove 502, and the movable angle of the positioning block 503 is 45°, so that the rotating clamp 202 drives the glass slide 7 to rotate between the horizontal and 45°, which is convenient for the staining and cleaning operations of the glass slide 7. Adsorption blocks 504 are fixedly embedded at both ends of the fan-shaped groove 502. The adsorption blocks 504 are adsorbed and correspond to the positioning blocks 503. The positioning blocks 503 and the adsorption blocks 504 are both made of magnetic materials. The positioning blocks 503 and the adsorption blocks 504 attract each other to achieve the fixation of the glass slide 7 in the inclined state and the horizontal state, thereby effectively improving the stability of the glass slide 7 during staining and cleaning;

[0041] During the transmission movement of the glass slide 7, the rotating wheel 5 contacts the tooth pad 501, driving the rotating clamp 202 to rotate, so that the glass slide 7 on the rotating clamp 202 is in a tilted state, specifically, the glass slide 7 is in a 45° tilted state, and then the rotating wheel 5 contacts the tooth pad 501 again, and the rotating clamp 202 drives the glass slide 7 to restore to a horizontal state. The glass slide 7 in the tilted state is convenient for cleaning operations, and the glass slide 7 in the horizontal state is convenient for dripping and infiltrating with a dye. The horizontal state and the tilted state of the glass slide 7 are both attracted and fixed by the positioning block 503 and the adsorption block 504, so as to maintain the state of the glass slide 7 stably.

[0042] Figure 9-10As shown, a glass slide pad 6 is slidably connected to the long end surface of the rotating clamp 202, and the glass slide pad 6 corresponds to the edge of the glass slide 7, and a spring 601 is fixedly connected between the glass slide pad 6 and the curved portion of the rotating clamp 202. When the glass slide 7 pushes the rotating clamp 202 horizontally, the glass slide pad 6 limits the glass slide 7, which is convenient for accurate clamping of the glass slide 7. An output push rod 602 is fixedly connected to the middle of the output platform 102, and a film taking hook 603 is connected to the output end of the output push rod 602. The film taking hook 603 corresponds to the glass slide pad 6 in parallel, and the top of the film taking hook 603 corresponds to the edge of the glass slide 7. When the rotating clamp 202 moves to the position of the output platform 102, the film taking hook 603 pushes away and replaces the glass slide pad 6, and the output push rod 602 subsequently pulls the film taking hook 603 to realize automatic removal of the glass slide 7.

[0043] When the glass slide 7 is pushed into the rotating clamp 202, the glass slide pad 6 is used to limit the edge of the glass slide 7, so that the rotating clamp 202 can accurately clamp the glass slide 7, which is convenient for positioning the staining position on the subsequent glass slide 7. When the rotating clamp 202 drives the glass slide 7 to move to the position of the output table 102, the glass slide pad 6 is displaced by the slide taking hook 603 and compresses the spring 601, so that the slide taking hook 603 pushes away and replaces the glass slide pad 6, and then the output push rod 602 pulls the slide taking hook 603 to pull the glass slide 7 out of the rotating clamp 202, which is convenient for taking out the glass slide 7 after staining, thereby effectively improving the production efficiency of the glass slide 7.

[0044] In view of current practical needs, the above-mentioned implementation mode adopted in this application is not limited to the scope of protection. Various changes made within the knowledge scope of technical personnel in this field without departing from the concept of this application still fall within the scope of protection of the present invention.

Claims

1. A high uniformity slide staining system, characterized in that: The system comprises a system track (1), wherein the movable end of the system track (1) is fixedly connected to slide modules (2) arranged at equal intervals, the middle of the slide module (2) is fixedly connected to a glass slide (7), and the top of the system track (1) is fixedly connected to staining modules (3) arranged at equal intervals, and the staining modules (3) correspond vertically to the slide modules (2); The front and rear ends of the system track (1) are respectively fixedly connected to an input platform (101) and an output platform (102), and the input platform (101) and the output platform (102) both correspond to the slide module (2). The plurality of dyeing modules (3) are arranged equidistantly from the input platform (101) to the output platform (102), and the middle of the second dyeing module (3) is fixedly connected to a dyeing positioning detection module (4), and the two ends of the dyeing module (3) are respectively fixedly connected to a dye dripping component (301) and a cleaning component (302), and the dye dripping component (301) is close to the input platform (101), and the dye dripping component (301) is fixedly connected to the detection end of the dyeing positioning detection module (4); The output ends of the input station (101) and the output station (102) are commonly connected to a glass slide recording unit, the input end of the glass slide recording unit is connected to a dyeing position recording unit, the input end of the dyeing position recording unit is connected to a dyeing positioning detection module (4), and the output end of the glass slide recording unit is connected to a control end of a dye dripping assembly (301); The slide module (2) comprises an end block (201), the end block (201) is fixedly connected to the movable end of the system track (1), the middle part of the end block (201) is rotatably connected to a rotating clamp (202), the rotating clamp (202) clamps and fixes the glass slide (7), the rotating clamp (202) is arranged in an L-shaped vertical bending, and a clamp (203) is hingedly connected to the top of the short end of the rotating clamp (202), the lower surface of the clamp (203) is in contact with the surface of the glass slide (7), the middle part of the clamp (203) is fixedly connected to a water injection port (204), the water outlet end of the cleaning component (302) is correspondingly facing the water injection port (204), and the lower surface of the clamp (203) is provided with a water seepage groove (205), and the water seepage groove (205) is connected to the water injection port (204); One end of the rotating clamp (202) close to the end block (201) is fixedly connected to a rotating wheel (5); the top and bottom of the system track (1) are alternately fixedly connected to tooth pads (501); the rotating wheel (5) is meshed with the tooth pads (501); the tooth groove length of the tooth pads (501) is one eighth of the circumference of the outer ring of the rotating wheel (5); the surface of the end block (201) is fixedly connected to a fan-shaped groove (502); the surface of the rotating wheel (5) is fixedly connected to a positioning block (503); the positioning block (503) is slidably connected to the fan-shaped groove (502); and the movable angle of the positioning block (503) is 45°.

2. A high uniformity slide staining system according to claim 1, characterized in that: The dye dripping assembly (301) is made of a T-shaped electric rail (303), and the movable end of the T-shaped electric rail (303) is fixedly connected to a dye barrel (304), and the dye positioning detection module (4) is fixed to the outside of the dye barrel (304).

3. A high uniformity slide staining system according to claim 1, characterized in that: Adsorption blocks (504) are fixedly embedded at both ends of the fan-shaped groove (502), the adsorption blocks (504) adsorb and correspond to the positioning blocks (503), and the positioning blocks (503) and the adsorption blocks (504) are both made of magnetic material.

4. A high uniformity slide staining system according to claim 1, characterized in that: A glass slide pad (6) is slidably connected to the long end surface of the rotating clamp (202), the glass slide pad (6) corresponds to the edge of the glass slide (7), and a spring (601) is fixedly connected between the glass slide pad (6) and the curved portion of the rotating clamp (202).

5. A high uniformity slide staining system according to claim 4, characterized in that: An output push rod (602) is fixedly connected to the middle of the output platform (102), and an output end of the output push rod (602) is connected to a slide hook (603), the slide hook (603) is parallel to the glass slide pad (6), and the top end of the slide hook (603) is corresponding to the edge of the glass slide (7).

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