A kind of aquatic animal lipid-lowering and blood-enriching compound preparation and its application

Abstract

The present application relates to the technical field of aquatic animals, and specifically to a compound preparation for reducing lipids and replenishing blood in aquatic animals and its application. The compound preparation comprises 40 to 60 parts of salvia miltiorrhiza, 15 to 25 parts of astragalus, 10 to 20 parts of cassia seed, 8 to 12 parts of chrysanthemum and 4 to 6 parts of hawthorn by weight. The compound preparation can regulate lipid metabolism in fish, reduce blood lipid levels, increase the number of blood cells and hemoglobin content in fish blood, improve blood oxygen carrying capacity, and can also improve the survival rate of aquatic animals.

Description

Technical Field

[0001] The present application relates to the technical field of aquatic animals, and in particular to a lipid-lowering and blood-enriching compound preparation of aquatic animals and its application. Background Art

[0002] Aquatic animals (such as grass carp) that are fed with feed often have excessive fat deposition in the liver. The widespread use of high-fat diets is an important reason for this consequence. Long-term and excessive use of high-fat diets often leads to lipid metabolism disorders in the body, excessive deposition of fat in the organs or tissues of the fish, increased blood lipids, lipotoxicity, and fatty liver. The liver is the largest metabolic and immune organ in the fish body. The occurrence of fatty liver often causes disorders in the body's various metabolic functions, thereby reducing the fish's immunity and anti-stress ability, and then causing bacterial and viral infections, causing large-scale and large-scale deaths and injuries.

[0003] During the peak growth season of fish, the liver of fish will also show symptoms of "anemia" with insufficient redness. The most numerous blood cell in fish's blood is red blood cells, which are the main medium for fish to transport oxygen through the blood. Hemoglobin is an iron-containing complex allosteric protein of red blood cells, whose function is to transport oxygen and carbon dioxide and maintain the acid-base balance of blood. The number of red blood cells and the hemoglobin content are indicators closely related to the body's breathing and nourishment conditions. When the fish grows too fast, the number of red blood cells and the hemoglobin content in the fish's blood are insufficient, and the blood's oxygen-carrying capacity cannot meet the oxygen demand of the rapidly growing fish, affecting its normal physiological functions, and the fish's resistance becomes worse. When the environment changes, it is easy to be infected with diseases. Summary of the invention

[0004] In order to solve the technical problems existing in the prior art, the inventors took into account that Chinese herbal medicines have the synergistic regulatory effects of multiple components, multiple targets and multiple pathways, and based on the traditional Chinese medicine theory, formulated and compounded the prescriptions and invented a compound preparation with "Salvia miltiorrhiza, Astragalus, Cassia seed, Chrysanthemum and Hawthorn" as ingredients, which has the effect of lowering lipids and tonifying blood.

[0005] To this end, the embodiments of the present application disclose at least the following technical solutions:

[0006] In a first aspect, an embodiment discloses a compound preparation for lowering lipids and replenishing blood in aquatic animals, which comprises, by weight, 40 to 60 parts of salvia miltiorrhiza, 15 to 25 parts of astragalus, 10 to 20 parts of cassia seed, 8 to 12 parts of chrysanthemum and 4 to 6 parts of hawthorn.

[0007] In some embodiments, the compound preparation comprises, by weight, 45 parts of salvia miltiorrhiza, 20 parts of astragalus, 18 parts of cassia seed, 10 parts of chrysanthemum and 5 parts of hawthorn.

[0008] In some embodiments, the compound preparation comprises, by weight, 50 parts of salvia miltiorrhiza, 20 parts of astragalus, 18 parts of cassia seed, 10 parts of chrysanthemum and 5 parts of hawthorn.

[0009] In some embodiments, the compound preparation comprises, by weight, 55 parts of salvia miltiorrhiza, 20 parts of astragalus, 18 parts of cassia seed, 10 parts of chrysanthemum and 5 parts of hawthorn.

[0010] In the second aspect, the embodiment discloses the application of the compound preparation of the first aspect, wherein the application is selected from at least one of the following: blood cell count, increasing hemoglobin content, reducing mortality during breeding, improving body resistance and / or reducing blood lipid levels. DETAILED DESCRIPTION

[0011] In order to make the purpose, technical scheme and advantages of the present application clearer, the present application is further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described herein are only used to explain the present application and are not intended to limit the present application. The reagents not described separately in detail in the present application are all conventional reagents and can be obtained from commercial channels; the methods not described in detail are all conventional experimental methods and can be obtained from the prior art.

[0012] Indoor test on efficacy of drugs on California bass

[0013] 1. Test samples of compound preparations

[0014] Compound preparation 1 is prepared by mixing 50 g of purple salvia miltiorrhiza, 20 g of astragalus, 15 g of cassia seed, 10 g of chrysanthemum and 5 g of hawthorn after being crushed.

[0015] Compound preparation 2 is prepared by mixing 45 g of salvia miltiorrhiza, 20 g of astragalus, 15 g of cassia seed, 10 g of chrysanthemum and 5 g of hawthorn after being crushed.

[0016] Compound preparation 3 is prepared by crushing and mixing 55 g of Salvia miltiorrhiza, 20 g of Astragalus, 15 g of Cassia seed, 10 g of Chrysanthemum and 5 g of Crataegus.

[0017] Compound preparation 4 is prepared by mixing 65 g of purple salvia miltiorrhiza, 10 g of astragalus, 8 g of cassia seed, 10 g of chrysanthemum and 5 g of hawthorn after being crushed.

[0018] Compound preparation 5 is prepared by crushing and mixing 50 g of Salvia miltiorrhiza, 20 g of Astragalus membranaceus and 15 g of Cassia seed.

[0019] Compound preparation 6 is prepared by crushing and mixing 50 g of Salvia miltiorrhiza, 20 g of Astragalus, 15 g of Cassia seed and 10 g of Chrysanthemum.

[0020] 2. Experimental animals and breeding management

[0021] California bass, purchased from Hubei Weilicheng Agricultural Technology Co., Ltd., was temporarily raised for one week with basic feed (Tianma California bass feed, Xiamen Zhengxia Biotechnology Co., Ltd.) before the start of the experiment. The experimental fish were randomly divided into 7 groups, 3 parallels in each group, and 30 fish in each parallel. The drug was administered by mixed feeding, and the amount of drug feed added was 2%. The control group was fed with basic feed, and the experimental groups were fed with mixed feed containing 2% of compound preparations 1 to 6, respectively, for 30 consecutive days.

[0022] 3. Sample collection

[0023] (1) Whole blood collection. At the end of the experiment, 5 fish were taken from each parallel group, and blood was collected from the tail vein. The blood was placed in a 1.5 mL EP tube (previously moistened with 1% sodium heparin), gently mixed, and placed in a 4°C refrigerator for red blood cell and hemoglobin determination.

[0024] (2) Serum collection. At the end of the experiment, five fish were taken from each parallel group, and blood was collected from the tail vein. After standing at room temperature for 1 hour, it was placed in a 4°C refrigerator overnight. Serum was collected by centrifugation at 3000 r / min (4°C) for 10 minutes and immediately stored in a -80°C refrigerator for the determination of serum biochemical indicators.

[0025] 5. Index determination

[0026] Hemoglobin content was determined by alkaline colorimetry; red blood cell counts were diluted 200 times with saline and counted under a microscope using a hemacytometer. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were determined using Nanjing Jiancheng kits.

[0027] 6. Results and Analysis

[0028] (1) Changes in blood physiological indicators of California bass

[0029] As can be seen from Table 1, the addition of compound preparations 1, 2, and 3 to the feed significantly increased the number of red blood cells, hemoglobin content, and white blood cells in the serum of California bass compared with the control group (P < 0.05). Among them, the number of red blood cells, hemoglobin content, and white blood cells in the serum of compound preparation 1 were the highest. The indicators of compound preparations 4, 5, and 6 were higher than those of the control group, but the difference was not significant (P > 0.05). Red blood cells can transport oxygen and carbon dioxide. The hemoglobin content in blood cells reflects the oxygen carrying capacity of blood. The increase in its content can indicate the enhancement of oxygen carrying capacity, indicating that compound preparations 1, 2, and 3 have the effect of increasing the number of blood cells and improving the body's oxygen transport capacity. Compound preparation 1 has the best effect. White blood cells have an important role in phagocytizing foreign bodies, producing antibodies, resisting pathogen invasion, and immunity. It shows that compound preparations 1, 2, and 3 have the effect of enhancing the body's immunity, among which compound preparation 1 has the best effect.

[0030] Table 1 Changes in blood physiological indicators of California bass

[0031]

[0032]

[0033] Note: "*" indicates significant difference compared with the control group (P<0.05).

[0034] (2) Changes in serum biochemical indicators of California bass

[0035] As can be seen from Table 2, the levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in the serum of California bass in compound preparation groups 1, 2 and 3 were significantly lower than those in the control group (P < 0.05), and the values ​​of each index in compound preparation groups 4, 5 and 6 were lower than those in the control group, but the difference was not significant (P > 0.05). The body's lipid metabolism capacity can be evaluated by the levels of TG, HDL-C, LDL-C and TC. HDL-C plays an important role in transporting TC and free fatty acids (FFA) from body tissues to liver cells for re-metabolism, while LDL-C transfers TC to peripheral tissue cells. The reduction of TG, TC, HDL-C and LDL-C in serum indicates that compound preparations 1, 2 and 3 can promote the body's fat metabolism and reduce blood lipid levels, among which compound preparation 1 has the best effect.

[0036] Table 2 Changes in blood biochemical indicators of California bass

[0037] Group TG (mmol / L) TC(mmol / L) HDL-C (mmol / L) LDL-C (mmol / L) Compound preparation 1 <![CDATA[2.25±0.27 * ]]> <![CDATA[6.53±0.35 * ]]> <![CDATA[6.65±0.32 * ]]> <![CDATA[2.57±0.27 * ]]> Compound preparation 2 <![CDATA[2.37±0.17 * ]]> <![CDATA[6.73±0.32 * ]]> <![CDATA[6.82±0.29 * ]]> <![CDATA[2.74±0.24 * ]]> Compound preparation 3 <![CDATA[2.41±0.15 * ]]> <![CDATA[6.79±0.29 * ]]> <![CDATA[6.97±0.41 * ]]> <![CDATA[2.93±0.32 * ]]> Compound preparation 4 2.73±0.22 6.92±0.25 7.25±0.36 3.52±0.29 Compound preparation 5 2.85±0.24 7.14±0.27 7.37±0.32 3.34±0.30 Compound preparation 6 2.76±0.18 7.06±0.30 7.31±0.25 3.21±0.26 Control group 2.94±0.24 7.16±0.27 7.42±0.23 3.68±0.28

[0038] Note: "*" indicates significant difference compared with the control group (P<0.05).

[0039] Combining the results of Tables 1 and 2, it can be seen that Compounds 1, 2, and 3 have the effects of increasing the number of blood cells, improving the body's resistance, and lowering blood lipid levels.

[0040] California bass pond experiment

[0041] 1. Test samples of compound preparations

[0042] Compound preparation 1 is prepared by mixing 50 g of purple salvia miltiorrhiza, 20 g of astragalus, 15 g of cassia seed, 10 g of chrysanthemum and 5 g of hawthorn after being crushed.

[0043] Compound preparation 2 is prepared by mixing 45 g of salvia miltiorrhiza, 20 g of astragalus, 15 g of cassia seed, 10 g of chrysanthemum and 5 g of hawthorn after being crushed.

[0044] Compound preparation 3 is prepared by crushing and mixing 55 g of Salvia miltiorrhiza, 20 g of Astragalus, 15 g of Cassia seed, 10 g of Chrysanthemum and 5 g of Crataegus.

[0045] Compound preparation 4 is prepared by mixing 65 g of purple salvia miltiorrhiza, 10 g of astragalus, 8 g of cassia seed, 10 g of chrysanthemum and 5 g of hawthorn after being crushed.

[0046] Compound preparation 5 is prepared by crushing and mixing 50 g of Salvia miltiorrhiza, 20 g of Astragalus membranaceus and 15 g of Cassia seed.

[0047] Compound preparation 6 is prepared by crushing and mixing 50 g of Salvia miltiorrhiza, 20 g of Astragalus, 15 g of Cassia seed and 10 g of Chrysanthemum.

[0048] 2. Datang test

[0049] The experiment was carried out at the California bass breeding farm in Tongshan, Xianning. Seven ponds with similar areas and fish numbers were selected as the control group and the experimental group. The control group was fed with basic feed (Tianma California bass feed, Xiamen Zhengxia Biotechnology Co., Ltd.), and the experimental group was fed with medicated feed (the addition of compound preparations 1 to 6 was 2%). The mixed feed was fed for 30 days, and the breeding conditions of California bass were recorded.

[0050] 3. Sample collection

[0051] (1) Whole blood collection. At the end of the experiment, 5 fish were taken from each pool, and blood was collected from the tail vein. The blood was placed in a 1.5 mL EP tube (previously moistened with 1% sodium heparin), gently mixed, and placed in a 4°C refrigerator for red blood cell and hemoglobin determination.

[0052] (2) Serum collection. At the end of the experiment, 5 fish were taken from each pool, and blood was collected from the tail vein. After standing at room temperature for 1 hour, it was placed in a 4°C refrigerator overnight. Serum was collected by centrifugation at 3000 r / min (4°C) for 10 minutes and immediately stored in a -80°C refrigerator for the determination of serum biochemical indicators.

[0053] 4. Index determination

[0054] Hemoglobin content was determined by alkaline colorimetry; red blood cell counts were diluted 200 times with saline and counted under a microscope using a hemacytometer. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were determined using Nanjing Jiancheng kits.

[0055] 5. Results

[0056] (1) Breeding conditions

[0057] The experiment was conducted from April to May. During the breeding period, farmers reported that the California bass in the drug group were more active in feeding and had stronger stress resistance. During the trial, a total of 36 fish died in the control group, 10-13 fish died in the compound preparations 1, 2, and 3 groups, and 28-31 fish died in the compound preparations 4, 5, and 6 groups, as shown in Table 3.

[0058] Table 3 Death of California bass during the experiment

[0059] Group Number of deaths (last) Compound preparation 1 10 Compound preparation 2 12 Compound preparation 3 13 Compound preparation 4 28 Compound preparation 5 31 Compound preparation 6 29 Control group 36

[0060] (2) Changes in blood indicators of California bass

[0061] As can be seen from Table 4, after 30 days of medication, the number of red blood cells, hemoglobin content, and white blood cell count in the blood of California bass in the compound preparations 1, 2, and 3 groups were significantly higher than those in the control group (P < 0.05), and the compound preparations 4, 5, and 6 were higher than the control group, but the difference was not significant (P > 0.05); the triglyceride, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol content in the serum of the compound preparations 1, 2, and 3 groups were significantly lower than those in the control group (P < 0.05), and the compound preparations 4, 5, and 6 were lower than the control group, but the difference was not significant (P > 0.05). It can be seen that compound preparations 1, 2, and 3 can increase the number of blood cells and hemoglobin content in the blood, improve the body's oxygen transport capacity, promote the body's lipid metabolism, and reduce blood lipid levels.

[0062] Table 4 Changes in blood indicators of California bass

[0063]

[0064] Note: "*" indicates significant difference compared with the control group (P<0.05).

[0065] In summary, feeding compound preparations 1, 2, and 3 mixed with feed can increase the number of blood cells and hemoglobin content, reduce blood lipid levels, improve the body's resistance, and reduce the mortality rate during the breeding process.

[0066] The above is only a preferred specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by any technician familiar with the technical field within the technical scope disclosed in the present application should be covered within the protection scope of the present application.

Claims

1. Use of a crushed mixture in preparing a compound preparation for increasing the number of blood cells in California seabass, increasing the hemoglobin content in California seabass, reducing the mortality rate in a California seabass breeding process, increasing the body resistance of California seabass and / or reducing the blood lipid level in California seabass, wherein the crushed mixture is prepared by weight from 40 to 60 parts of salvia miltiorrhiza, 15 to 25 parts of astragalus, 10 to 20 parts of cassia seed, 8 to 12 parts of chrysanthemum and 4 to 6 parts of hawthorn.

2. The use according to claim 1, wherein the crushed mixture is made of 45 parts of Salvia miltiorrhiza, 20 parts of Astragalus, 18 parts of Cassia seed, 10 parts of Chrysanthemum and 5 parts of Crataegus by weight.

3. The use according to claim 1, wherein the crushed mixture is made of 50 parts of Salvia miltiorrhiza, 20 parts of Astragalus, 18 parts of Cassia seed, 10 parts of Chrysanthemum and 5 parts of Crataegus by weight.

4. The use according to claim 1, wherein the crushed mixture is made of 55 parts of Salvia miltiorrhiza, 20 parts of Astragalus, 18 parts of Cassia seed, 10 parts of Chrysanthemum and 5 parts of Crataegus by weight.

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