A self-flocculating water purifying algae and its application

Abstract

The present invention belongs to the technical field of water treatment and resource utilization, and in particular relates to a self-flocculating water purifying algae and its application. Nannochloropsis sp. YTLJ‑NAPE‑SF1; The present invention adopts a special self-flocculating water purification algae species, which can realize self-flocculation and precipitation in the water purification process. While purifying nitrogen and phosphorus pollutants in the water, it has a combined removal effect on toxic pollutants such as microplastics and heavy metals.

Description

Technical Field

[0001] The invention relates to the technical field of water treatment and resource utilization, in particular to self-flocculating water-purifying algae and application thereof. Background Art

[0002] High-intensity human activities have brought about problems such as complex pollution in the water environment. Currently, there is a relative lack of biological technologies that can remove complex pollution in water. Traditional biological water purification technologies based on nitrogen-metabolizing bacteria usually have a certain effect on nitrogen removal, but it is difficult to achieve the coordinated removal of multiple pollutants and is prone to cause the accumulation of heavy metals, which greatly limits the promotion and application of such technologies.

[0003] At present, the removal of pollutants such as nutrients in water mainly relies on bacterial biological treatment. However, bacterial denitrification and phosphorus removal faces problems such as complex processes and easy inhibition by high dissolved oxygen. In recent years, microalgae in-situ water purification technology has been used in the water treatment industry to a certain extent. This technology can achieve rapid removal of nitrogen and phosphorus nutrients in water and play a role in purifying water quality. However, microalgae usually grow in water in a suspended state, facing many problems such as difficulty in precipitation and removal from water, and high suspended solids (SS) in the back-end effluent. Summary of the invention

[0004] The purpose of the present invention is to provide a self-flocculating water purifying algae and its application in view of the deficiencies of the prior art.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] A self-flocculating water purifying algae, the self-flocculating water purifying algae is Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 was deposited in the General Microbiology Center of China Microbiological Culture Collection Administration on December 27, 2023, with the deposit number CGMCC No.40800 and the classification name Nannochloropsis Nannochloropsis sp. , the storage address is Beijing, China.

[0007] An application of the self-flocculating water purification algae, the Nannochloropsis Nannochloropsis sp. Application of YTLJ-NAPE-SF1 in self-flocculation.

[0008] An application of the self-flocculating water purification algae, the Nannochloropsis Nannochloropsis sp. Application of YTLJ-NAPE-SF1 in the removal of microplastics and / or heavy metals in the environment.

[0009] The heavy metal is Cu and / or As.

[0010] An application of the self-flocculating water purification algae, the Nannochloropsis Nannochloropsis sp. Application of YTLJ-NAPE-SF1 in water purification.

[0011] A self-flocculating water purification algae agent, the algae agent containing the Nannochloropsis described in claim 1 Nannochloropsis sp. YTLJ-NAPE-SF1.

[0012] The algae agent is Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 is expanded and then mixed with the auxiliary agent at a mass ratio of 1-100:1; the total effective number of live algae in the algae agent is ≥1.0×10 9 Pieces / mL.

[0013] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 is dried, crushed, and then mixed with additives in proportion.

[0014] The auxiliary agent is sodium humate (commercially available), nitromycin-attapulgite, and bisphenol A complex mixed at a mass mixing ratio of 0.01-0.1:1:0.0001-0.00001; wherein the nitromycin-attapulgite complex is prepared by mixing nitromycin and attapulgite at a mass ratio of 1:50-200 through ball milling for standby use.

[0015] The advantages of the present invention are:

[0016] The present invention overcomes many shortcomings of traditional microalgae water purification technology by adding special algae species to strengthen self-flocculation purification, specifically:

[0017] 1) The obtained algae species can solve the problem of difficult flocculation and sedimentation removal in the process of microalgae treatment of aquaculture tail water in an eco-friendly way through self-flocculation and sedimentation, thereby reducing suspended matter in the effluent while purifying the water.

[0018] 2) The algae species of the present invention are easy to grow and proliferate on the plastic surface, can promote the co-flocculation and precipitation of microplastics, and provide a new idea for the removal of microplastic pollution.

[0019] 3) The present invention adopts a special self-flocculating water purification algae species, which can remove typical heavy metals in the water while purifying nitrogen and phosphorus pollutants in the tail water, and can solve the complex pollution problem in aquaculture water in an eco-friendly manner. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The Nannochloropsis algae provided by the embodiment of the present invention Nannochloropsis sp. Self-flocculation diagram of YTLJ-NAPE-SF1 during cultivation; Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 (left) has self-flocculation function compared with common microalgae (right, commercial Chlorella). DETAILED DESCRIPTION

[0021] The present invention is further described below by way of examples, however the present invention is not limited to the following examples.

[0022] The attapulgite described in the following Examples is commercially available attapulgite. Example 1

[0023] Nannochloropsis Nannochloropsis sp. Isolation of YTLJ-NAPE-SF1:

[0024] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 was isolated from the coastal environment. The specific isolation process was as follows: 10 g of seawater sample (without filtration) was placed in 50 mL of sterile seawater and oscillated at 25 °C for 48 h. The bacterial solution was centrifuged at 8000 rpm for 10 min, and the upper clear liquid was removed. The lower algae solution was transferred to a sterilized enrichment medium. After culturing at 30 °C for 7 days, it was diluted and cultured in LB medium. Single algae were selected and separated by streaking on agar solid medium to obtain purified Nannochloropsis spp. Nannochloropsis sp. YTLJ-NAPE-SF1.

[0025] The enrichment medium used was 0.05% NH4NO3, 0.05% NH4NO2, 0.2% sodium humate, 0.1% sodium hexanoate, 2% K2HPO4, 3% MgSO4, 1% NaCl, 0.01% FeSO4, 0.01% MnSO 4、 0.01% bisphenol A and 0.005% azomycin.

[0026] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 was deposited in the General Microbiology Center of China Microbiological Culture Collection Administration, Beijing, China, on December 27, 2023, with the deposit number CGMCC No.40800;

[0027] By comparing the 23S rDNA gene sequence, it was confirmed that Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 is a newly discovered algae species.

[0028] Nannochloropsis Nannochloropsis sp. The 23S rDNA gene sequence of YTLJ-NAPE-SF1 is shown in SEQ ID NO: 1:

[0029] TGGTCCCAAAGCGATGATCTGGTCTTTACTTGCGCAGAATAGGTGGGAGGCATTGAAGAAAATCTTGTGGGATTTTTGGAGCCAATCGTGAGATACCACTCTTTAAAGCTCGGATTCTCACTCTCTACCGTAATCCGGGGAGAGGACACTCCCAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGG TAACGGAGGCGCACAAAGGTTTCCTCAGATCGGATAGAAATCGATCGAAGAGGTGTAAAGGCAAAAGGAAGCTTGACTGCGAGACGTACAAGTCAAGCAGAGACGAAAGTCGGTCTTAGTGATCCGACGATCCTGAGTGGAAAGGTCGTCGCTCAACGGATAAAAGTTACTCTAGGGATAACGGGGCTGATCTA.

[0030] Nannochloropsis Nannochloropsis sp. Application of YTLJ-NAPE-SF1 in water treatment system:

[0031] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 was cultured in enriched medium until OD 750 The bacterial solution with a value of 1 was inoculated into the simulated nitrogen-containing aquaculture tail water at an inoculation rate of 10wt%. After 4 weeks of treatment at 25℃, the concentrations of ammonia nitrogen, nitrite, nitrate nitrogen, and phosphate dropped to 0.1, 0.14, 4, and 0.05 mg / L, respectively. The abundance of microplastics was reduced to 1.0×10 8 MPs×mL -1 The concentrations of heavy metals Cu and As were reduced to 0.04 mg / L and 0.01 mg / L, respectively. SS decreased from 150 mg / L to 20 mg / L. At the same time, commercially available Chlorella vulgaris (purchased from Nanhua Qianmu) was used as a control (see Figure 1 ).

[0032] The nitrogen-containing aquaculture tail water is ammonia nitrogen, nitrite, nitrate, phosphate, microplastics, and heavy metals added per liter of water, wherein the concentrations of ammonia nitrogen, nitrite, nitrate nitrogen, and phosphate are 1 mg / L, 1 mg / L, 50 mg / L, and 0.5 mg / L, respectively, and the abundance of microplastics (average size 1 mm) added is 1.0×10 9 MPs / mL, and the concentrations of heavy metals Cu and As were 0.5 mg / L and 0.06 mg / L, respectively.

[0033] Depend on Figure 1 It can be seen that NannochloropsisNannochloropsis sp. YTLJ-NAPE-SF1 can overcome the shortcomings of ordinary microalgae water purification and achieve self-flocculation sedimentation and control of complex pollution. Example 2

[0034] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 is used for tail water treatment in an eel farm in Shandong:

[0035] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 was propagated under the conditions of 25°C, pH 7.5, and 2000 lx of light. 750 The value is 1.

[0036] The expanded Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 algae liquid is freeze-dried and crushed to make algae powder, which is then mixed with additives at a mass ratio of 100:1 to obtain algae agent. The total effective number of live algae in the algae agent is ≥1.0×10 9 Pieces / mL.

[0037] The algae agent obtained above was added to the eel breeding tailwater pond at a dosage of 1wt%. Four weeks after the breeding started at 25°C, the removal rates of ammonia nitrogen, nitrite, nitrate and phosphate in the breeding tailwater exceeded 90%, the SS removal rate was 80%, the microplastic removal rate was 80%, and the heavy metal Cu removal rate was 90%.

[0038] The auxiliary agent is a mixture of sodium humate, nitromycin-attapulgite complex and bisphenol A in a mass ratio of 0.01:1:0.0001; the nitromycin-attapulgite complex is a mixture of nitromycin and attapulgite in a ratio of 1:50, which is then vacuum dried after ultrasonic treatment for later use.

[0039] The initial concentrations of ammonia nitrogen, nitrite, nitrate, phosphate, SS, and Cu in the eel breeding tailwater pond were 0.8, 0.05, 17.8, 5.6, 250, and 0.2 mg / L, respectively. Example 3

[0040] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 is used for tail water treatment of Penaeus vannamei aquaculture:

[0041] The algae powder obtained above was mixed with the auxiliary agent at a mass ratio of 50:1 to prepare an algae agent, and the total number of effective live algae in the algae agent was ≥1.0×10 9 Pieces / mL.

[0042] The algae agent obtained above was added to the ecological ditch of shrimp aquaculture tailwater at an addition amount of 2 wt%. One week after the breeding started at 25℃, the removal rates of ammonia nitrogen, nitrite, nitrate and phosphate in the aquaculture pond were 80%. The removal rates of SS, microplastics and heavy metal Cu were 70%, 70%, and 80%, respectively.

[0043] The auxiliary agent is a mixture of sodium humate, nitromycin-attapulgite complex and bisphenol A in a mass ratio of 0.05:1:0.00005; the nitromycin-attapulgite complex is a mixture of nitromycin and attapulgite in a mass ratio of 1:100, which is subjected to ultrasonic treatment and then vacuum dried for later use.

[0044] The initial concentrations of ammonia nitrogen, nitrite, nitrate, phosphate, SS, microplastics, and Cu in the shrimp aquaculture tailwater ecological ditch were 1.6 mg / L, 0.14 mg / L, 18 mg / L, 2.5 mg / L, 600 mg / L, 0.40 mg / L, and 2.4×10 9 MPs / mL. Example 4

[0045] Nannochloropsis Nannochloropsis sp. YTLJ-NAPE-SF1 is used for municipal tail water treatment:

[0046] The algae powder obtained above was mixed with the auxiliary agent in a mass ratio of 1:1 to prepare an algae agent, and the total number of effective live algae in the algae agent was ≥ 1.0×10 9 Pieces / mL.

[0047] The algae agent obtained above was added to the municipal tail water at an addition amount of 0.1wt%. After 8 weeks of breeding at 20℃, the removal rates of ammonia nitrogen, nitrite, nitrate and phosphate in the aquaculture tail water were 70%. The removal rate of microplastics was 90%. The removal rate of SS was 80%, and the removal rate of heavy metals Cu and As was 60%.

[0048] The auxiliary agent is a mixture of sodium humate, azomycin-attapulgite composite and bisphenol A in a mass ratio of 0.1:1:0.00001; the azomycin-attapulgite composite is a mixture of azomycin and attapulgite in a mass ratio of 1:200, which is subjected to ultrasonic treatment and then vacuum dried for later use.

[0049] The initial concentrations of ammonia nitrogen, nitrite, nitrate, phosphate, SS, Cu, and microplastics in the municipal tail water were 5.4 mg / L, 0.4 mg / L, 10 mg / L, 1.0 mg / L, 100 mg / L, 0.18 mg / L, and 1.7×10 9 MPs / mL.

Claims

1. A self-flocculating water purification algae, characterized in that: The self-flocculating water-purifying algae is Nannochloropsis ( Nannochloropsis sp. )YTLJ-NAPE-SF1, deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 27, 2023, with the deposit number CGMCC No.40800, the classification name is Nannochloropsis, and the deposit address is Beijing, China.

2. An application of the self-flocculating water purification algae according to claim 1, characterized in that: The application of the Nannochloropsis algae YTLJ-NAPE-SF1 in removing microplastics and / or heavy metals in aquatic environments.

3. The use of the self-flocculating water purifying algae according to claim 2, characterized in that: The heavy metal is Cu and / or As.

4. An application of the self-flocculating water purification algae according to claim 1, characterized in that: The application of the Nannochloropsis algae YTLJ-NAPE-SF1 in purifying water bodies.

5. A self-flocculating algae purifier, characterized in that: The algae agent contains the Nannochloropsis YTLJ-NAPE-SF1 described in claim 1.

6. The self-flocculating algae purifier according to claim 5, characterized in that: The algae agent is obtained by propagating Nannochloropsis YTLJ-NAPE-SF1, and then mixing it with an auxiliary agent at a mass ratio of 1-100:1; wherein the total effective number of live algae in the algae agent is ≥1.0×10 9 Pieces / mL.

7. The self-flocculating algae purifier according to claim 6, characterized in that: The Nannochloropsis algae YTLJ-NAPE-SF1 is expanded, dried, crushed, and then mixed with an auxiliary agent in proportion.

8. The self-flocculating algae purifier according to claim 6, characterized in that: The auxiliary agent is a mixture of sodium humate, nitromycin-attapulgite and bisphenol A compound in a mass ratio of 0.01-0.1:1:0.0001-0.00001; wherein the nitromycin-attapulgite compound is prepared by mixing nitromycin and attapulgite in a mass ratio of 1:50-200 through ball milling for standby use.

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