Waste discharge structure and biochip

Abstract

The application discloses a kind of waste discharge structures, including waste liquid cavity, water-absorbing material A is contained in the waste liquid cavity, the liquid inlet of the waste liquid cavity is communicated with waste discharge pipeline B, the liquid inlet of waste discharge pipeline B is equipped with waste discharge valve, water-absorbing material A includes water-soluble film packaging bag and granular water-absorbing material D contained in the water-soluble film packaging bag, the liquid outlet of the waste liquid cavity is sequentially communicated with waste discharge pipeline C, water-absorbing material B, suction negative pressure port;Multiple liquid inlets are provided in the waste liquid cavity and / or multiple waste liquid cavities are connected in parallel.The waste discharge can reach 8~9 times by using the new parallel multi-outlet waste discharge mode, and the outlet is completely blocked in the smallest waste liquid cavity volume, the waste discharge target is completed, and efficient waste discharge is realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biochips, in particular to a waste discharge structure and a biochip. BACKGROUND

[0002] A biochip is a kind of chip that integrates sample preparation, reaction, separation, detection and other basic operation units of biological, chemical and medical analysis processes on a small chip to automatically complete the whole analysis process. Therefore, the biochip technology instrument is relatively simple, has no liquid path, and is easy to maintain or maintenance-free.

[0003] During the whole analysis process of the biochip, waste liquid is generated. The waste liquid left in the chip is relatively friendly to the instrument and the customer. However, due to some special functional requirements, the waste liquid discharge can only be discharged by using a negative pressure power source. In the case of waste liquid discharge by using negative pressure, the waste liquid chamber needs to be designed to lock and trap water, so that after the waste liquid enters the waste liquid chamber, the negative pressure cannot continue to suck the waste liquid out of the chip. Therefore, water-absorbing material needs to be designed in the waste liquid chamber of the chip. After absorbing water, the water-absorbing material will change the water into a part of the water-absorbing material through physical or chemical means, which can resist the suction force of the negative pressure.

[0004] A negative pressure waste liquid suction device and a biochip are disclosed in Chinese Patent No. CN215833418U. The main part of the negative pressure waste liquid suction device is a waste liquid collection chamber. One end of the waste liquid collection chamber is in communication with a liquid inlet, and a normally closed micro valve is arranged at the liquid inlet. The other end is in communication with the negative pressure suction port of the base, and a normally open micro valve is arranged at the negative pressure suction port. The negative pressure generating device is connected to the negative pressure suction port. A vacuum negative pressure waste liquid collection chamber is formed by controlling the on-off of the micro valve. The waste liquid is caused to flow into the waste liquid collection chamber by the negative pressure suction force and is absorbed by the water-absorbing material in the chamber. The patent has the following characteristics during the waste discharge process:

[0005] (1) During the negative pressure waste discharge process, the waste liquid flows in the direction of air flow according to the principle of fluid dynamics;

[0006] (2) The waste liquid cannot pass through the water-absorbing material to be discharged into the negative pressure pump, and the entry of the waste liquid into the negative pressure pump will damage the negative pressure pump;

[0007] (3) After the water-absorbing material absorbs water, it will expand or form a dense material, blocking the waste discharge path, so that the waste discharge cannot continue, the chip cannot reach the designed volume, and the chip is scrapped.

[0008] When the patent has only one fixed waste discharge port, the waste liquid chamber can only discharge waste liquid for 3-4 times, and the outlet of the waste liquid chamber is blocked. SUMMARY

[0009] The technical problem solved by the present application is to provide a waste discharge structure and a biochip, which can complete the waste discharge target and realize efficient waste discharge under the condition of the smallest waste liquid cavity volume.

[0010] To solve the above technical problems, the technical solution of the present application is as follows: a waste discharge structure, comprising a waste liquid cavity, wherein the waste liquid cavity contains water-absorbing material A, the waste liquid cavity is connected with waste discharge pipeline B through a liquid inlet, the waste discharge pipeline B is provided with a waste discharge valve at the liquid inlet, the water-absorbing material A comprises a water-soluble film packaging bag and granular water-absorbing material D contained in the water-soluble film packaging bag, and the waste liquid cavity is sequentially connected with waste discharge pipeline C, water-absorbing material B and negative pressure suction port through a liquid outlet.

[0011] The waste liquid cavity is provided with two or more liquid inlets, and / or the waste liquid cavity is provided with multiple waste discharge pipelines in parallel.

[0012] In one embodiment of the present application, the waste liquid cavity is provided with multiple liquid inlets, when the water-absorbing material A contacts the waste liquid, the water-soluble film packaging bag contacts the waste liquid first, and the waste liquid dissolves the water-soluble film packaging bag in a few seconds, and in the dissolution time, the waste liquid is absorbed by the water-absorbing material B. After the first waste discharge, the water-soluble film packaging bag of the water-absorbing material A is dissolved, and the granular water-absorbing material D leaks out. Then, during the second waste discharge, the liquid inlet close to the waste discharge valve absorbs more water than the liquid inlet far from the waste discharge valve, and expands first. The volume of the front expansion gradually increases, resulting in gradually increasing pressure at the liquid inlet outlet. According to fluid mechanics, at this time, the waste liquid will preferentially flow to the place with small pressure at the liquid inlet outlet for waste discharge, and when the pressure difference of all liquid inlets of the waste discharge pipeline B8 is similar, the waste liquid will be discharged from the front liquid inlet. When all the water-absorbing resins are expanded to block the path near each liquid inlet, the final waste discharge failure will occur, which can effectively utilize the volume of the waste liquid cavity and realize efficient waste discharge.

[0013] In one embodiment of the present application, the waste liquid chambers are connected in parallel, and when the water-absorbing material A contacts the waste liquid, the water-soluble film packaging bag contacts the waste liquid first, and the waste liquid dissolves the water-soluble film packaging bag in a few seconds. During the dissolving time, the waste liquid is absorbed by the water-absorbing material B. After the first waste discharge, the water-soluble film packaging bag of the water-absorbing material A dissolves, and the granular water-absorbing material D leaks out. Then, during the second waste discharge, the waste liquid inlet of the waste liquid chamber close to the waste discharge valve absorbs more water than the waste liquid inlet far from the waste discharge valve, and expands first. The volume of the front expansion gradually increases, causing the pressure of the waste liquid inlet close to the waste discharge valve to gradually increase. According to fluid mechanics, at this time, the waste liquid will preferentially flow to the place with lower pressure at the inlet outlet, and when the pressure difference of all inlet outlets on the waste discharge pipeline B8 is similar, the waste liquid will be discharged from the front inlet again. When the water-absorbing resin in all chambers expands to block the path near each inlet, the final waste discharge failure occurs, which can effectively utilize the volume of the waste liquid chamber and achieve efficient waste discharge.

[0014] In the present application, the water-absorbing material A is packaged with a water-soluble film packaging bag, which is not easily sucked into the vacuumizing device at the beginning of vacuumizing. The water-soluble film has water-soluble characteristics and is soluble in cold water. The water-soluble speed can be designed and selected, and it is non-toxic and non-polluting.

[0015] In a preferred embodiment of the present application, the inlet is arranged close to the water-absorbing material A. When the water-soluble film packaging bag dissolves, the granular water-absorbing material D leaks out and absorbs the waste liquid at the inlet.

[0016] In a preferred embodiment of the present application, the water-absorbing material A is provided in multiple bags and is fixed at each inlet. Each water-soluble film packaging bag is dissolved after contacting the waste liquid, and the granular water-absorbing material D leaks out in turn, and then absorbs the waste liquid. The inlet close to the waste discharge valve absorbs more water than the inlet far from the waste discharge valve, and expands first. The multiple bags of water-absorbing material A are fully utilized to absorb the waste liquid until all the water-absorbing resin expands to block the path near each inlet.

[0017] In a preferred embodiment of the present application, the number of waste liquid discharges required by different test items is usually 5-7 times, and 6-8 inlets are arranged in the waste liquid chamber.

[0018] In a preferred embodiment of the present application, the granular water-absorbing material D is water-absorbing resin microparticles.

[0019] In a preferred embodiment of the present application, the water-soluble film packaging bag is a PVA film, which has a dissolving time of about 4-10 seconds after contacting the waste liquid.

[0020] In a preferred embodiment of the present invention, the absorbent material B is absorbent paper, which is a material that can quickly absorb water.

[0021] In a preferred embodiment of the present invention, the waste discharge valve is connected to the atmosphere and / or to an atmosphere duct.

[0022] The present invention also discloses a biochip, including a waste discharge structure, wherein the inlet of the waste discharge valve is connected to the reaction chamber, and an inlet valve is installed at the inlet of the reaction chamber.

[0023] In a preferred embodiment of the present invention, the two ends of the atmospheric vent pipe are respectively connected to the reaction chamber and the waste discharge valve.

[0024] Compared with existing technologies, the beneficial effects of this invention are as follows: By employing a novel parallel multi-outlet waste discharge method, waste discharge can be completed in 8-9 cycles before the outlet is completely blocked. The waste discharge target is achieved with a minimal waste liquid chamber volume, thus realizing highly efficient waste discharge. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the structure of the biochip in Embodiment 1 of the present invention.

[0026] Figure 2 This is a schematic diagram of the structure of the biochip in Embodiment 2 of the present invention.

[0027] Among them, 1. Liquid inlet valve; 2. Liquid inlet pipeline; 3. Reaction chamber; 4. Vent pipe; 5. Waste discharge pipeline A; 6. Vent port; 7. Waste discharge valve; 8. Waste discharge pipeline B; 9. Waste liquid chamber; 10. Absorbent material A; 11. Waste discharge pipeline C; 12. Absorbent material B; 13. Negative pressure port; 14. Liquid inlet; 15. Liquid inlet II. Detailed Implementation

[0028] Example 1

[0029] like Figure 1 As shown, a waste discharge structure includes a waste liquid chamber 9, which contains absorbent material A10. The inlet of the waste liquid chamber 9 is connected to a waste discharge pipe B8, and the inlet of the waste discharge pipe B8 is equipped with a waste discharge valve 7. The outlet of the waste liquid chamber 9 is sequentially connected to a waste discharge pipe C11, absorbent material B12, and a negative pressure port 13. The granular absorbent material D is absorbent resin microparticles. The water-soluble film packaging bag is PA film. The absorbent material B12 is absorbent paper. The waste liquid chamber 9 has seven inlets 14. Each inlet 14 is located adjacent to the absorbent material A10. Multiple bags of absorbent material A10 are disposed and fixed at each of the inlets 14. The waste discharge valve 7 is connected to the atmosphere and / or to an atmosphere-venting pipe 4.

[0030] The reaction cavity 3 is actually a plurality of reaction chambers. The liquid reagent after each reaction needs to be discharged into the waste liquid chamber 9. The volume of the waste liquid chamber is 9000ul, and the volume of the waste liquid discharged each time is 800-900ul. According to different test items, the number of times of discharging waste liquid is different from 5 to 7 times. The waste liquid chamber 9 cannot block the waste discharge pipeline B8 before the detection is completed. Blocking will cause the waste liquid in the subsequent reaction cavity 3 to be unable to be discharged, so that the subsequent reaction cannot be carried out.

[0031] The waste discharge workflow of the embodiment: the biological chip is used for synchronous reaction of a plurality of reaction cavities. The power source of reagent sampling and waste discharge is a negative pressure power source. The negative pressure power source performs negative pressure suction on the chip reaction cavity 3, the waste liquid chamber 9, and the corresponding liquid inlet pipeline 2, waste discharge pipeline A, waste discharge pipeline B, and atmosphere pipeline, so that these regions are all in a negative pressure state. Then the liquid inlet valve 1 is opened, and the reagent stored in the chip enters the reaction cavity 3 through the liquid inlet valve 1 and the liquid inlet pipeline 2. After sampling is completed, the sampling valve 1 is closed.

[0032] After the reaction is completed, the liquid inlet valve 1 is in a closed state, and the state of the waste discharge valve 7 is opened to a waste liquid state. At this time, the waste discharge pipeline A5 is connected to the waste discharge pipeline B8 through the waste discharge valve 7. The atmosphere pipeline 4 is connected to the atmosphere port 6 through the waste discharge valve 7. The negative pressure power source performs negative pressure suction through the negative pressure suction port 13. The liquid in the reaction cavity enters the waste liquid chamber 9 through the waste discharge pipeline A5, the waste discharge valve 7, and the waste discharge pipeline B8 under the pressure difference between the atmospheric pressure of the atmosphere port 6 of the reaction cavity and the negative pressure. The waste liquid is absorbed by the water absorption material A10 after contacting the water absorption material A10. The water absorption material A10 can trap water by physically or chemically allowing water to become part of the water absorption material A10, so that the water cannot be sucked by negative pressure. In this example, the water absorption material A10 is preferably water absorption resin microparticles, which can quickly absorb water and expand into a gel state.

[0033] The special features of the present invention: if it is only a conventional waste, and then the water absorbing resin absorbs water and expands, it is easy to think. But what are the defects? If the waste liquid cavity 9 filled with water absorbing material A10 for bulk powder, will be difficult to produce, and the water absorbing material can not be fixed, there is a risk of being negative pressure. Secondly, because the liquid in the negative pressure under the movement of the airflow direction, if the waste liquid pipeline only one waste, because the waste liquid all to one direction, then the water absorbing material A10 local absorption of water too much, after the expansion will be to the negative pressure port 13 path ahead of the blockage, resulting in waste liquid cavity 9 in many places volume waste, low efficiency. To solve these problems, first in the waste liquid cavity with two kinds of water absorbing material, water absorbing material A10 and water absorbing material B12, water absorbing material A10 for bagged packaging form, packaging material for water soluble film, the present example is preferred PVA film. Water absorbing material B12 for fast water absorbing material, the present example is preferred water absorbing paper. Another in the waste pipeline B8 from front to back parallel with 7 liquid inlet 14. The purpose of this is, in the waste, PVA film after the water about 4~10 seconds of dissolution time, so in the waste liquid cavity 9 to the negative pressure port 13 of the public part of the design of water absorbing material B12 can absorb the first few seconds out of the waste liquid. After the first time, the water absorbing material A10 packaging bag has been dissolved, leakage of water absorbing resin particles. Then in the second time, the waste, the front several liquid inlet 14 (close to the waste valve 7 of the liquid inlet 14) than the water absorbing water absorbing material B12 (distance from the waste valve 7 of the liquid inlet 14) more, will be first swelling. The volume of the front swelling gradually increases, leading to the pressure of liquid inlet 14 outlet gradually increases. According to fluid mechanics, the waste liquid will be preferentially run to the liquid inlet 14 outlet pressure small place for waste, when the waste pipeline B8 on all the liquid inlet 14 outlet pressure difference is about the same time, also began to first from the front of the liquid inlet 14. When the difference is about all the chamber of water absorbing resin swelling to the road near each liquid inlet 14 are blocked, it will cause the final can't waste, so you can maximize the effective use of the volume of the waste liquid cavity 9, realize efficient waste.

[0034] Example 2

[0035] The difference between Example 1 and Example 2 is that the waste liquid chamber 9 is parallelly arranged with 7, and each waste liquid chamber is respectively provided with the water absorbing material A 10. When the water absorbing material A contacts the waste liquid, the water-soluble film packaging bag contacts the waste liquid first, and the waste liquid makes the water-soluble film packaging bag dissolve for several seconds, and in the several seconds of dissolution time, the waste liquid is absorbed by the water absorbing material B. After the first waste discharge, the water-soluble film packaging bag of the water absorbing material A dissolves, and the granular water absorbing material D leaks out. Then in the second waste discharge, the waste liquid chamber inlet 14 close to the waste discharge valve 7 absorbs more water than the waste liquid chamber inlet (such as inlet II 15) far away from the waste discharge valve, and expands first. The volume of the previous expansion gradually increases, causing the pressure of the waste liquid chamber inlet 14 close to the waste discharge valve to gradually increase. According to fluid mechanics, at this time, the waste liquid will preferentially discharge to the place where the outlet pressure of the inlet (such as inlet II 15) is small, and when the pressure difference of all the inlets on the waste discharge pipeline B8 is similar, it will start to discharge from the previous inlet (such as inlet 14) first. When the water absorbing resin in all the chambers expands to block the way near each inlet, the final failure to discharge will be caused, which can maximize the effective use of the volume of the waste liquid chamber 9 and achieve efficient waste discharge.

Claims

1. A waste discharge structure, comprising a waste liquid chamber (9), wherein the waste liquid chamber (9) contains absorbent material A (10), the inlet (14) of the waste liquid chamber (9) is connected to a waste discharge pipe B (8), and the inlet of the waste discharge pipe B (8) is equipped with a waste discharge valve (7), characterized in that, The absorbent material A (10) includes a water-soluble film packaging bag and granular absorbent material D contained in the water-soluble film packaging bag. The outlet of the waste liquid chamber (9) is connected in sequence to the waste discharge pipe C (11), the absorbent material B (12), and the negative pressure port (13). The waste liquid chamber (9) is provided with two or more inlets (14) and / or multiple waste liquid chambers (9) are arranged in parallel; The liquid inlet (14) is located immediately adjacent to the absorbent material A (10); The absorbent material A (10) is provided in multiple bags, and the number of absorbent materials A (10) corresponds one-to-one with the number of liquid inlets (14), and is fixed at each of the liquid inlets (14); The granular absorbent material D is micro-particles of absorbent resin.

2. The waste discharge structure according to claim 1, characterized in that, The waste liquid chamber (9) is provided with 6-8 inlets (14).

3. The waste discharge structure according to any one of claims 1-2, characterized in that, The water-soluble film packaging bag is a PVA film.

4. The waste discharge structure according to any one of claims 1-2, characterized in that, The absorbent material B (12) is absorbent paper.

5. The waste discharge structure according to any one of claims 1-2, characterized in that, The waste discharge valve (7) is connected to the atmosphere and / or to the atmosphere duct (4).

6. A biochip, characterized in that... The waste discharge structure includes any one of claims 1-5, wherein the inlet of the waste discharge valve (7) is connected to the reaction chamber (3), and an inlet valve (1) is installed at the inlet of the reaction chamber (3).

7. The biochip according to claim 6, characterized in that, The two ends of the atmospheric duct (4) are respectively connected to the reaction chamber (3) and the waste discharge valve (7).

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