Pipette calibration device and method of use thereof

Abstract

The present invention relates to the technical field of pipette calibration technology, and specifically discloses a pipette calibration device and a method for using the same, comprising a base, wherein the base is connected to a turntable via a rotating motor, the turntable is slidably connected to a clamping assembly having multiple groups of pipettes, a first bracket and a second bracket are respectively provided on one side of the turntable, the first bracket is provided with an automatic liquid taking mechanism that cooperates with the pipette on the clamping assembly, and the second bracket is provided with an automatic calibration mechanism that cooperates with the pipette on the clamping assembly; the automatic liquid taking mechanism and the automatic correction mechanism in the present invention cooperate with the turntable to continuously complete automatic liquid taking, automatic calibration, and automatic liquid discharge operations of multiple pipettes. Compared with the traditional manual calibration operation of a single pipette, the present pipette calibration device has diversified functions, can continuously perform calibration operations on multiple pipettes, and improves the calibration efficiency of the pipette.

Description

Technical Field

[0001] The present invention relates to the technical field of pipette calibration, and in particular to a pipette calibration device and a use method thereof. Background Art

[0002] A pipette, also known as a pipette gun, is an instrument used for quantitatively transferring liquids. Pipettes are commonly used to transfer small or trace amounts of liquids in analytical testing and research. Pipettes can be categorized by their operating principle as either gas piston pipettes or external piston pipettes. Gas piston pipettes are primarily used for standard pipetting, while external piston pipettes are primarily used for handling volatile, corrosive, and viscous liquids.

[0003] Currently, pipette calibration is usually done manually, which involves many steps. Calibration of a pipette takes at least ten minutes, which is not only inefficient but also very tiring for continuous operation of simple and multiple steps, thus affecting the calibration accuracy of the pipette.

[0004] Therefore, we provide a pipette calibration device and a method of using the same to solve the above problems. Summary of the Invention

[0005] (1) Technical issues to be resolved

[0006] The problem to be solved by the present invention is to provide a pipette calibration device and a method of using the same, so as to overcome the defects in the prior art.

[0007] (2) Technical solution

[0008] To solve the above technical problems, the present invention provides a pipette calibration device, comprising a base, the base being connected to a turntable via a rotating motor, the turntable being slidably connected to a clamping assembly having multiple groups of pipettes, a first bracket and a second bracket being respectively provided on one side of the turntable, the first bracket being provided with an automatic liquid extraction mechanism that cooperates with the pipette on the clamping assembly, and the second bracket being provided with an automatic calibration mechanism that cooperates with the pipette on the clamping assembly;

[0009] The turntable is provided with a plurality of matching grooves in a circumferential array, the clamping assembly includes an electric clamping jaw slidably connected to the matching groove, and limit grooves are provided on both side walls of the matching groove. Limiting portions slidably connected to the limit grooves are provided on both sides of the electric clamping jaw, and a V-shaped clamping block is hingedly connected to the connecting rod of the electric clamping jaw. The electric clamping jaw in the clamping assembly clamps the pipette via the V-shaped clamping block, and the turntable drives the pipettes on the plurality of electric clamping jaws to perform circular trajectory displacement on one side of the first bracket and the second bracket;

[0010] The automatic liquid dispensing mechanism includes a first lifting cylinder mounted on a first bracket, a telescopic end of the first lifting cylinder fixedly connected to a connecting seat, two ends of the connecting seat are respectively connected to a first push rod and a second push rod, one end of the first push rod and the second push rod are respectively slidably connected to a first pressure head and a second pressure head, a second spring is provided between the first push rod and the first pressure head and between the second push rod and the second pressure head, the first pressure head corresponds to the upper end of the electric clamp, the second pressure head corresponds to the button on the pipette, and a sensor corresponding to the button is provided on the first bracket;

[0011] The automatic calibration mechanism includes a second lifting cylinder mounted on a second bracket, a telescopic end of the second lifting cylinder is fixedly connected to a third pressure head, a through hole is opened on the second bracket, a slide is fixedly connected to the second bracket on one side of the through hole, the slide is slidably connected to a slide, an electric push rod cooperating with the slide is fixedly connected to the slide, one end of the slide is fixedly connected to a reduction motor, the other end of the slide is rotatably connected to a rotating shaft, a rotating wheel corresponding to the adjustment knob on the pipette is fixedly connected to the rotating shaft, and a rubber ring is provided on the outer ring of the rotating wheel.

[0012] Furthermore, eight matching grooves are provided on the turntable, and a limiting column is provided on the bottom wall of each matching groove. A first spring is sleeved on each limiting column, and the first spring is located between the electric clamp and the bottom wall of the matching groove.

[0013] Furthermore, the upper end of each of the matching grooves is connected to a limit block via a bolt, and the limit block is located above the electric clamp.

[0014] Furthermore, the length of the first push rod is greater than the length of the second push rod.

[0015] Furthermore, a weighing pan is provided between the first bracket and the base, a liquid collection bucket is provided on the weighing pan, a recycling bucket is provided between the second bracket and the base, and a unscrew is provided on the pipette.

[0016] Furthermore, when the sensor detects the button of the pipette, the tip of the pipette close to the first bracket is located above the liquid collection bucket, and the tip of the pipette close to the second bracket is located above the recovery bucket.

[0017] A method for using a pipette calibration device comprises the following steps:

[0018] S1. Fix multiple pipettes on the V-shaped clamping blocks of the electric clamp respectively; control the rotary motor on the base to drive the turntable to move the pipette to the automatic liquid collection mechanism through the controller;

[0019] S2. When the sensor in the automatic liquid dispensing mechanism detects the button, the sensor detection signal is transmitted to the controller. After receiving the signal, the controller controls the turntable to stop running, controls the first lifting cylinder to drive the connecting seat to descend, and causes the first pressure head on the first push rod to press down the electric clamping claw, so that the electric clamping claw drives the pipette to move toward the liquid dispensing bucket;

[0020] S3, while the first pressure head presses down the electric clamp, the second pressure head on the second push rod presses down the button. When the electric clamp hits the limit column, the controller controls the first lifting cylinder to rise, and the first and second pressure heads are separated from the button and the electric clamp, so that the pipette extracts the solution in the liquid barrel. The electric clamp returns to its original position under the action of the first spring force. The controller controls the turntable to operate again, so that the sensor waits to detect the button of the next pipette that has not yet extracted liquid, and the pipette that has completed the liquid extraction operation moves to the automatic calibration mechanism.

[0021] S4. The scale weighs the weight of the liquid collection bucket in real time and transmits the weighing data to the controller synchronously. When the pipette completes the automatic liquid collection operation, the controller obtains the weight of the solution extracted by the pipette, thereby obtaining the volume of the extracted liquid in the pipette; the electric push rod in the automatic calibration mechanism drives the slide to move in the direction close to the pipette, so that the rubber ring of the rotating wheel contacts the adjustment knob of the pipette; when the controller obtains that the data value of the extracted volume in the pipette is less than the set calibration threshold, the reduction motor drives the rotating wheel on the rotating shaft to rotate forward through the transmission belt, and the rotating wheel drives the adjustment knob to rotate reversely to increase the extracted volume of the pipette; when the controller obtains that the data value of the extracted volume in the pipette is greater than the set calibration threshold, the reduction motor drives the rotating wheel on the rotating shaft to rotate reversely through the transmission belt, and the rotating wheel drives the adjustment knob to rotate forward to reduce the extracted volume of the pipette;

[0022] S5. After the automatic correction mechanism completes the correction operation of the pipette, the controller controls the electric push rod to drive the slide to return to its original position, and the second lifting cylinder drives the third pressure head to move down and press the button to discharge the extracted liquid in the pipette into the recovery bucket; repeat steps S1-S5 to complete the automatic liquid collection, automatic calibration, and automatic liquid discharge operations of multiple pipettes on the turntable.

[0023] (3) Beneficial effects

[0024] Compared with the prior art, the pipette calibration device and the method of use provided by the present invention have the following beneficial effects:

[0025] The present invention provides an automatic clamping assembly, which includes an electric clamping jaw that is slidably connected to a matching groove, and a limiting groove is provided on both side walls of the matching groove. Limiting parts that are slidably connected to the limiting groove are provided on both sides of the electric clamping jaw, and the connecting rod of the electric clamping jaw is hinged with a V-shaped clamping block. The electric clamping jaw in the clamping assembly clamps the pipette through the V-shaped clamping block, and the turntable drives the pipettes on the multiple electric clamping jaws to make circular trajectory displacements on the first bracket and one side of the first bracket, and the multiple pipettes are respectively fixed on the V-shaped clamping blocks of the electric clamping jaw, and the V-shaped clamping blocks hingedly connected to the connecting rod can well clamp irregular pipette bodies. The controller controls the rotating motor on the base to drive the turntable to operate, so that the multiple pipettes can be automatically moved to the automatic liquid taking mechanism and the automatic calibration mechanism, without the need for manual independent operation of the multiple pipettes, saving the operator's time for liquid taking and calibration, and improving the calibration efficiency of the pipette.

[0026] When the sensor in the automatic liquid dispensing mechanism of the present invention detects the button, the sensor detection signal is transmitted to the controller. After receiving the signal, the controller controls the turntable to stop operating, controls the first lifting cylinder to drive the connecting seat to descend, causes the first pressure head on the first push rod to press down the electric clamp, and causes the electric clamp to drive the pipette to move toward the liquid taking bucket; while the first pressure head presses the electric clamp, the second pressure head on the second push rod presses the button. When the electric clamp hits the limit column, the controller controls the first lifting cylinder to rise, and the first and second pressure heads are separated from the button and the electric clamp, causing the pipette to extract the solution in the liquid taking bucket. The electric clamp returns to its original position under the action of the first spring force, and the controller controls the turntable to operate again, causing the sensor to wait for detecting the button of the next pipette that has not yet taken liquid, so that the pipette that has completed the liquid taking operation moves to the automatic calibration mechanism, so that the liquid taking and calibration operations of multiple pipettes are carried out synchronously, thereby further improving the calibration efficiency of multiple pipettes.

[0027] The weighing pan in the present invention weighs the weight of the liquid collection bucket in real time and synchronously transmits the weighing data to the controller. When the pipette completes the automatic liquid collection operation, the controller obtains the weight of the solution extracted by the pipette, thereby obtaining the volume of the extracted liquid in the pipette; the electric push rod in the automatic calibration mechanism drives the slide to move in the direction close to the pipette, so that the rubber ring of the rotating wheel contacts the adjusting knob of the pipette; when the controller obtains that the data value of the extracted volume in the pipette is less than the set calibration threshold, the reduction motor drives the rotating wheel on the rotating shaft to rotate forward through the transmission belt, and the rotating wheel drives the adjusting knob to rotate reversely to increase the extracted volume of the pipette; when the controller obtains that the data value of the extracted volume in the pipette is greater than the set calibration threshold, the reduction motor drives the rotating wheel on the rotating shaft to rotate reversely through the transmission belt, and the rotating wheel drives the adjusting knob to rotate forward to reduce the extracted volume of the pipette; thereby achieving the effect of flexible, fast and portable calibration of the pipette, and the overall calibration mechanism has a simple structure and no complicated design.

[0028] 4. When the automatic correction mechanism completes the correction operation of the pipette, the controller controls the electric push rod to drive the slide to return to its original position, and the second lifting cylinder drives the third pressure head to move down and press the button to discharge the extracted liquid in the pipette into the recovery bucket; thereby completing the automatic liquid collection, automatic calibration, and automatic liquid discharge operations of multiple pipettes on the turntable. Compared with the traditional single pipette calibration operation, this pipette calibration device has diversified functions and can perform calibration operations on multiple pipettes continuously. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0030] Figure 1 It is a first stereoscopic view of a pipette calibration device according to the present invention;

[0031] Figure 2 A second stereoscopic view of a pipette calibration device according to the present invention;

[0032] Figure 3 This is a schematic diagram of the working of an automatic liquid dispensing mechanism of a pipette calibration device of the present invention;

[0033] Figure 4 This is a schematic diagram of the working of an automatic calibration mechanism of a pipette calibration device of the present invention;

[0034] Figure 5 This is a schematic diagram of the connection between the turntable and the electric gripper of a pipette calibration device of the present invention;

[0035] Figure 6 This is a schematic diagram of the separation of the turntable and the electric gripper of a pipette calibration device of the present invention;

[0036] Figure 7 This is a schematic diagram of the turntable structure of a pipette calibration device of the present invention;

[0037] Figure 8 This is a schematic diagram of the electric gripper structure of a pipette calibration device of the present invention;

[0038] Figure 9 A schematic diagram of the pipette structure of a pipette calibration device of the present invention;

[0039] Figure 10 This is a schematic diagram of an automatic liquid dispensing mechanism of a pipette calibration device of the present invention;

[0040] Figure 11 A schematic diagram of an automatic calibration mechanism of a pipette calibration device according to the present invention;

[0041] Figure 12 This is a partial structural diagram of an automatic calibration mechanism of a pipette calibration device according to the present invention;

[0042] The names of the components corresponding to the reference numerals in the figures are: 1. base; 2. turntable; 201. matching groove; 202. limiting groove; 203. limiting column; 204. first spring; 205. limiting block; 3. electric clamp; 301. limiting portion; 302. connecting rod; 303. V-shaped clamp; 4. pipette; 401. button; 402. untwist; 403. adjusting knob; 5. first bracket; 501. Sensor; 6. First lifting cylinder; 7. Connecting seat; 701. First push rod; 7011. First pressure head; 702. Second push rod; 7021. Second pressure head; 703. Second spring; 8. Second bracket; 9. Second lifting cylinder; 10. Slide; 11. Electric push rod; 12. Slide; 1201. Reducer motor; 1202. Rotating shaft; 1203. Rotating wheel; 14. Weighing pan; 16. Third pressure head. DETAILED DESCRIPTION

[0043] The present application is described in detail below with reference to the accompanying drawings and specific embodiments.

[0044] The following describes the embodiments of the present application through specific examples, and those skilled in the art can easily understand other advantages and effects of the present application from the contents disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The present application can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that, in the absence of conflict, the features in the following embodiments and embodiments can be combined with each other. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of this application.

[0045] It should be noted that various aspects of the embodiments within the scope of the appended claims are described below. It should be apparent that the aspects described herein can be embodied in a wide variety of forms, and any specific structure and / or function described herein is merely illustrative. Based on this application, it should be understood by those skilled in the art that an aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspect described herein can be used to implement an apparatus and / or practice a method. In addition, other structures and / or functionalities other than one or more of the aspects described herein can be used to implement this apparatus and / or practice this method.

[0046] It should also be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present application. The illustrations only show components related to the present application and are not drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component can be changed at will, and the component layout type may also be more complicated.

[0047] Additionally, in the following description, specific details are provided to provide a thorough understanding of the examples, however, one skilled in the art will appreciate that the examples can be practiced without these specific details.

[0048] The following describes the technical solutions provided by various embodiments of the present application in conjunction with the accompanying drawings.

[0049] See Figures 1 to 12 The present invention provides a pipette calibration device, comprising a base 1, the base 1 being connected to a turntable 2 via a rotating motor, the turntable 2 being slidably connected to a plurality of clamping assemblies for clamping pipettes 4, a first bracket 5 and a second bracket 8 being respectively provided on one side of the turntable 2, the first bracket 5 being provided with an automatic liquid taking mechanism cooperating with the pipette 4 on the clamping assembly, and the second bracket 8 being provided with an automatic calibration mechanism cooperating with the pipette 4 on the clamping assembly;

[0050] A plurality of mating grooves 201 are formed in a circular array on the turntable 2. The clamping assembly includes an electric clamping jaw 3 that is slidably connected to the mating groove 201. Limiting grooves 202 are formed on both side walls of the mating groove 201. Limiting portions 301 that are slidably connected to the limiting grooves 202 are provided on both sides of the electric clamping jaw 3. A V-shaped clamping block 303 is hingedly connected to the connecting rod 302 of the electric clamping jaw 3. The electric clamping jaw 3 in the clamping assembly clamps the pipette 4 via the V-shaped clamping block 303. The turntable 2 drives the pipettes 4 on the plurality of electric clamping jaws 3 to move in a circular trajectory on one side of the first bracket 5 and the second bracket 8.

[0051] The automatic liquid dispensing mechanism includes a first lifting cylinder 6 mounted on the first bracket 5, the telescopic end of the first lifting cylinder 6 is fixedly connected to the connecting base 7, the two ends of the connecting base 7 are respectively connected to the first push rod 701 and the second push rod 702, one end of the first push rod 701 and the second push rod 702 are respectively slidably connected to the first pressure head 7011 and the second pressure head 7021, a second spring 703 is provided between the first push rod 701 and the first pressure head 7011 and the second push rod 702 and the second pressure head 7021, the first pressure head 7011 corresponds to the upper end of the electric clamp 3, the second pressure head 7021 corresponds to the button 401 on the pipette 4, and the first bracket 5 is provided with a sensor 501 corresponding to the button 401;

[0052] The automatic calibration mechanism includes a second lifting cylinder 9 installed on the second bracket 8, the telescopic end of the second lifting cylinder 9 is fixedly connected to the third pressure head 16, the second bracket 8 is provided with a through hole, the second bracket 8 is located on one side of the through hole and is fixedly connected to a slide 10, the slide 10 is slidably connected to the slide 12, the slide 10 is fixedly connected to the electric push rod 11 that cooperates with the slide 12, one end of the slide 12 is fixedly connected to the reduction motor 1201, the other end of the slide 12 is rotatably connected to the rotating shaft 1202, the rotating shaft 1202 is fixedly connected to a rotating wheel 1203 corresponding to the adjusting knob 403 on the pipette 4, and the outer ring of the rotating wheel 1203 is provided with a rubber ring.

[0053] See Figures 3 to 12 There are eight matching grooves 201 on the turntable 2, and a limiting column 203 is provided on the bottom wall of each matching groove 201. A first spring 204 is sleeved on each limiting column 203. The first spring 204 is located between the electric clamp 3 and the bottom wall of the matching groove 201. The upper end of each matching groove 201 is connected to a limiting block 205 by a bolt. The limiting block 205 is located above the electric clamp 3. The length of the first push rod 701 is greater than the length of the second push rod 702.

[0054] See Figures 3 to 11 A weighing pan 14 is provided between the first bracket 5 and the base 1, a liquid collection bucket is provided on the weighing pan 14, a recovery bucket is provided between the second bracket 8 and the base 1, and a release button 402 is provided on the pipette 4. When the sensor 501 detects the button 401 of the pipette 4, the tip of the pipette 4 close to the first bracket 5 is located above the liquid collection bucket, and the tip of the pipette 4 close to the second bracket 8 is located above the recovery bucket.

[0055] A method for using a pipette calibration device comprises the following steps:

[0056] S1. Fix multiple pipettes 4 on the V-shaped clamping blocks 303 of the electric clamp 3 respectively; control the rotary motor on the base 1 to drive the turntable 2 to operate, so that the pipettes 4 move to the automatic liquid collection mechanism;

[0057] S2. When the sensor 501 in the automatic liquid dispensing mechanism detects the button 401, the sensor 501 transmits the detection signal to the controller. After receiving the signal, the controller controls the turntable 2 to stop operating, controls the first lifting cylinder 6 to drive the connecting seat 7 to descend, and causes the first pressure head 7011 on the first push rod 701 to press down the electric clamp 3, so that the electric clamp 3 drives the pipette 4 to move toward the liquid dispensing bucket;

[0058] S3, while the first pressure head 7011 presses down the electric clamp 3, the second pressure head 7021 on the second push rod 702 presses down the button 401. When the electric clamp 3 hits the limit column 203, the controller controls the first lifting cylinder 6 to rise, and the first pressure head 7011 and the second pressure head 7021 are separated from the button 401 and the electric clamp 3, so that the pipette 4 extracts the solution in the liquid collection barrel. The electric clamp 3 returns to its original position under the elastic force of the first spring 204. The controller controls the turntable 2 to operate again, so that the sensor 501 waits to detect the button 401 of the next pipette 4 that has not yet extracted liquid, so that the pipette 4 that has completed the liquid extraction operation moves to the automatic calibration mechanism;

[0059] S4, the scale 14 weighs the weight of the liquid barrel in real time and transmits the weighing data to the controller synchronously. When the pipette 4 completes the automatic liquid extraction operation, the controller obtains the weight of the solution extracted by the pipette 4, thereby obtaining the volume of the extracted liquid in the pipette 4; the electric push rod 11 in the automatic calibration mechanism drives the slide 12 to move in the direction close to the pipette 4, so that the rubber ring of the wheel 1203 contacts the adjustment knob 403 of the pipette 4; when the controller obtains the data value of the extracted volume in the pipette 4 is less than When the set calibration threshold is reached, the reduction motor 1201 drives the rotating wheel 1203 on the rotating shaft 1202 to rotate forward through the transmission belt, and the rotating wheel 1203 drives the adjusting knob 403 to rotate reversely to increase the extraction volume of the pipette 4; when the controller obtains that the extraction volume data value in the pipette 4 is greater than the set calibration threshold, the reduction motor 1201 drives the rotating wheel 1203 on the rotating shaft 1202 to rotate reversely through the transmission belt, and the rotating wheel 1203 drives the adjusting knob 403 to rotate forward to reduce the extraction volume of the pipette 4;

[0060] S5. After the automatic correction mechanism completes the correction operation of the pipette, the controller controls the electric push rod 11 to drive the slide 12 to return to its original position, and the second lifting cylinder 9 drives the third pressure head 16 to move down and press the button 401 to discharge the extracted liquid in the pipette 4 into the recovery bucket; repeat steps S1-S5 to complete the automatic liquid collection, automatic calibration, and automatic liquid discharge operations of multiple pipettes 4 on the turntable 2.

[0061] The same or similar parts between the various embodiments in this specification can be referred to each other, and each embodiment focuses on the differences from other embodiments.

[0062] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present application should be included in the scope of protection of the present application. Therefore, the scope of protection of the present application should be based on the scope of protection of the claims.

Claims

1. A pipette calibration device, comprising a base (1), characterized in that: The base (1) is connected to a turntable (2) via a rotating motor, and the turntable (2) is slidably connected to a plurality of clamping assemblies for clamping pipettes (4). A first bracket (5) and a second bracket (8) are respectively provided on one side of the turntable (2), and an automatic liquid taking mechanism cooperating with the pipette (4) on the clamping assembly is provided on the first bracket (5), and an automatic calibration mechanism cooperating with the pipette (4) on the clamping assembly is provided on the second bracket (8); The turntable (2) is provided with a plurality of matching grooves (201) in a circumferential array, the clamping assembly includes an electric clamp (3) slidably connected to the matching groove (201), and the two side walls of the matching groove (201) are provided with limiting grooves (202), and the two sides of the electric clamp (3) are provided with limiting portions (301) slidably connected to the limiting grooves (202), and the connecting rod (302) of the electric clamp (3) is hinged with a V-shaped clamping block (303), and the electric clamp (3) in the clamping assembly clamps the pipette (4) through the V-shaped clamping block (303), and the turntable (2) drives the pipettes (4) on the plurality of electric clamps (3) to move in a circular trajectory on one side of the first bracket (5) and the second bracket (8); The automatic liquid taking mechanism comprises a first lifting cylinder (6) mounted on a first bracket (5); a telescopic end of the first lifting cylinder (6) is fixedly connected to a connecting seat (7); two ends of the connecting seat (7) are respectively connected to a first push rod (701) and a second push rod (702); one end of the first push rod (701) and the second push rod (702) are respectively slidably connected to a first pressure head (7011) and a second pressure head (7021); a second spring (703) is provided between the first push rod (701) and the first pressure head (7011) and between the second push rod (702) and the second pressure head (7021); the first pressure head (7011) corresponds to the upper end of the electric clamp (3); the second pressure head (7021) corresponds to the button (401) on the pipette (4); and a sensor (501) corresponding to the button (401) is provided on the first bracket (5); The automatic calibration mechanism includes a second lifting cylinder (9) mounted on a second bracket (8), a telescopic end of the second lifting cylinder (9) is fixedly connected to a third pressure head (16), a through hole is provided on the second bracket (8), and a slide (10) is fixedly connected to the second bracket (8) on one side of the through hole, the slide (10) is slidably connected to a slide (12), an electric push rod (11) that cooperates with the slide (12) is fixedly connected to the slide (10), one end of the slide (12) is fixedly connected to a reduction motor (1201), and the other end of the slide (12) is rotatably connected to a rotating shaft (1202), a rotating wheel (1203) corresponding to the adjustment knob (403) on the pipette (4) is fixedly connected to the rotating shaft (1202), and the outer ring of the rotating wheel (1203) is provided with a rubber ring; Eight matching grooves (201) are provided on the turntable (2), and a limiting column (203) is provided on the bottom wall of each matching groove (201). A first spring (204) is sleeved on each limiting column (203), and the first spring (204) is located between the electric clamp (3) and the bottom wall of the matching groove (201); A weighing pan (14) is provided between the first bracket (5) and the base (1), a liquid collection bucket is provided on the weighing pan (14), a recovery bucket is provided between the second bracket (8) and the base (1), and a release screw (402) is provided on the pipette (4); When the sensor (501) detects the button (401) of the pipette (4), the tip of the pipette (4) close to the first bracket (5) is located above the liquid collection barrel, and the tip of the pipette (4) close to the second bracket (8) is located above the recovery barrel.

2. The pipette calibration device according to claim 1, wherein: The upper end of each matching groove (201) is connected to a limit block (205) via a bolt, and the limit block (205) is located above the electric clamp (3).

3. The pipette calibration device according to claim 1, wherein: The length of the first push rod (701) is greater than the length of the second push rod (702).

4. A method for using a pipette calibration device, applied to the pipette calibration device according to any one of claims 1 to 3, characterized in that: The steps include: S1. Fixing a plurality of pipettes (4) on the V-shaped clamping blocks (303) of the electric clamping jaws (3) respectively; controlling the rotary motor on the base (1) by the controller to drive the turntable (2) to operate, so that the pipettes (4) move to the automatic liquid collection mechanism; S2. When the sensor (501) in the automatic liquid taking mechanism detects the button (401), the sensor (501) transmits a detection signal to the controller. After receiving the signal, the controller controls the turntable (2) to stop running, controls the first lifting cylinder (6) to drive the connecting seat (7) to descend, and causes the first pressure head (7011) on the first push rod (701) to press down the electric clamp (3), so that the electric clamp (3) drives the pipette (4) to move toward the liquid taking barrel; S3, while the first pressure head (7011) presses down the electric clamp (3), the second pressure head (7021) on the second push rod (702) presses down the button (401). When the electric clamp (3) contacts the limit column (203), the controller controls the first lifting cylinder (6) to rise, and the first pressure head (7011) and the second pressure head (7021) are separated from the button (401) and the electric clamp (3), so that the pipette (4) extracts the solution in the liquid collection barrel. The electric clamp (3) returns to its original position under the elastic force of the first spring (204). The controller controls the turntable (2) to operate again, so that the sensor (501) waits to detect the button (401) of the next pipette (4) that has not yet taken liquid, so that the pipette (4) that has completed the liquid collection operation moves to the automatic calibration mechanism; S4, the weighing pan (14) weighs the weight of the liquid collection barrel in real time and transmits the weighing data to the controller synchronously. When the pipette (4) completes the automatic liquid collection operation, the controller obtains the weight of the solution extracted by the pipette (4), thereby obtaining the volume of the liquid extracted in the pipette (4); the electric push rod (11) in the automatic calibration mechanism drives the slide (12) to move in the direction close to the pipette (4), so that the rubber ring of the rotating wheel (1203) contacts the adjustment knob (403) of the pipette (4); when the controller obtains the data value of the volume extracted in the pipette (4) which is less than the set value, the controller turns to the control panel (11). When the calibration threshold is reached, the reduction motor (1201) drives the rotating wheel (1203) on the rotating shaft (1202) to rotate forward through the transmission belt, and the rotating wheel (1203) drives the adjusting knob (403) to rotate reversely to increase the extraction volume of the pipette (4); when the controller obtains that the extraction volume data value in the pipette (4) is greater than the set calibration threshold, the reduction motor (1201) drives the rotating wheel (1203) on the rotating shaft (1202) to rotate reversely through the transmission belt, and the rotating wheel (1203) drives the adjusting knob (403) to rotate forward to reduce the extraction volume of the pipette (4); S5. After the automatic correction mechanism completes the correction operation of the pipette, the controller controls the electric push rod (11) to drive the slide (12) to return to its original position, and the second lifting cylinder (9) drives the third pressure head (16) to descend and press the button (401), so that the extracted liquid in the pipette (4) is discharged into the recovery bucket; repeat steps S1-S5 to complete the automatic liquid collection, automatic calibration, and automatic liquid discharge operations of multiple pipettes (4) on the turntable (2).

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