Developing stain and method for developing biological traces

Abstract

The present application provides a developing dye and a method for developing biological traces, which belongs to the field of identification and detection technology, and in particular to the field of detection technology of trace organic matter. The raw materials of the developing dye include: rhodamine 6G, anhydrous ethanol and hydrogen peroxide; wherein, the developing dye includes the following raw materials when prepared in a volume of 100 ml: 0.025g to 0.05g of rhodamine 6G, 85 to 95 ml of anhydrous ethanol mixed with 5 to 15 ml of 30% hydrogen peroxide. The present application can solve the problem of poor biological trace development effect during the inspection process, and there is no need to distinguish in advance whether the biological trace is a blood latent trace or a sweat latent trace, and a better development effect can be obtained.

Description

Technical Field

[0001] The present application relates to the field of identification and detection technology, in particular to the field of detection technology of trace organic matter, and specifically to a developing dye and a visualization method for biological traces. Background Art

[0002] Fingerprint visualization is the basis for fingerprint recognition, analysis and identification. Scientifically and correctly discovering, extracting and displaying fingerprints plays an important role in conducting investigations and punishing crimes. How to make the biological traces of the target object left on non-permeable objects, such as blood-latent or sweat-latent fingerprints, more completely visible has become a hot topic in the industry.

[0003] At present, in the existing technology, the traditional methods include optical visualization (such as ultraviolet light inspection, laser inspection, etc.), physical adsorption (such as powder brushing, iodine fumigation, etc.) and chemical visualization (such as silver nitrate method, cyanoacrylate fumigation technology, ninhydrin method, 1,8-diaza-9-fluorenone method, etc.). However, when detecting biological traces with traditional methods, there are more than one type of residues in the biological traces left by the target object, and the traditional methods used for different residues are also different. Different methods will interfere with each other, resulting in poor visualization of biological traces. Summary of the invention

[0004] The present application provides a developing stain and a method for developing biological traces, which are used to solve the problem of poor biological trace development effect during the inspection process.

[0005] In a first aspect, the present application provides a developing dye for biological traces, characterized in that the raw materials of the developing dye include: rhodamine 6G, anhydrous ethanol and hydrogen peroxide;

[0006] The developing dye comprises the following raw materials when prepared in a volume of 100 ml: 0.025 g to 0.05 g of rhodamine 6G, 85-95 ml of anhydrous ethanol mixed with 5-15 ml of 30% hydrogen peroxide.

[0007] In a possible implementation, the raw materials of the developing dye further include: methanol, acetic acid, ethyl acetate and glycerol;

[0008] The developing dye comprises the following raw materials when prepared in a volume of 100 ml: 0.025 g to 0.05 g of rhodamine 6G, 4.5 ml of methanol, 2 ml of acetic acid, 2.5 ml of ethyl acetate, 1 ml of glycerol, 85.5 ml of anhydrous ethanol and 4.5 ml of 30% hydrogen peroxide.

[0009] In a second aspect, the present application also provides a method for displaying biological traces, comprising the following steps:

[0010] Loading the developing dye as described in the first aspect into an ultrasonic atomizing device, and placing the nozzle of the ultrasonic atomizing device at a preset distance from the object to be developed, with the nozzle facing the object to be developed;

[0011] Turning on the ultrasonic atomization device to atomize the developing dye from a liquid state into an aerosol state, so as to spray the developing dye in the aerosol state for a preset time onto the object to be developed;

[0012] The light source is turned on, so that the potential biological traces on the surface of the object to be displayed can be observed by an observer wearing cut-off filter glasses.

[0013] In a possible implementation, after turning on the light source, the method further includes: turning on an image acquisition device, wherein a cutoff filter is installed on the camera of the image acquisition device; and photographing the potential biological traces on the surface of the object to be displayed through the image acquisition device to obtain target trace image data.

[0014] In a possible implementation, photographing the potential biological traces on the surface of the object to be displayed by the image acquisition device includes: adjusting the photographing focal length of the image acquisition device to photograph the potential biological traces on the surface of the object to be displayed.

[0015] In one possible implementation, the spray rate of the nozzle is 0.15 ml / min to 0.30 ml / min, the ultrasonic operating frequency of the ultrasonic atomization device is 1 MHz to 3 MHz; the preset distance is 10 cm to 15 cm, the particle size of the developing dye in the aerosol state is 3.0 μm ± 25%, and the preset time is 5 s to 10 s.

[0016] In one possible implementation, the ultrasonic atomization device is turned on to atomize the developer dye from a liquid state into an aerosol state so as to spray the developer dye in the aerosol state for a preset time length onto the object to be developed, comprising: turning on the ultrasonic atomization device, controlling the operating frequency of the ultrasonic atomization device, and adjusting the average particle size of the developer dye in the aerosol state so as to spray the developer dye in the aerosol state for a preset time length onto the object to be developed.

[0017] In a possible implementation, the operating frequency of the ultrasonic atomization device is 1.67 MHz, and the average particle size of the developing dye in the aerosol state is 2.582 μm.

[0018] In a possible implementation, the fluorescence wavelength generated by the light source is 450 to 530 nm.

[0019] In a possible implementation, the ultrasonic atomization device, the light source, and the image acquisition device are integrated into a developer, which also includes a power supply device and a control device. The power supply device is electrically connected to the ultrasonic atomization device, the light source, the image acquisition device, and the control device, and the control device is communicatively connected to the ultrasonic atomization device, the light source, and the image acquisition device.

[0020] The present application provides a developing dye and a method for developing biological traces, wherein the developing dye, through the concentration range of the developing dye prepared according to the above embodiment, can make the surface of the object to be displayed show clear and complete fingerprint lines. And different types of fingerprints have strong fluorescence characteristics. When using the developing dye for biological traces provided in the embodiment of the present application for biological trace inspection, there is no need to distinguish in advance whether the biological trace is a blood latent trace or a sweat latent trace, and a better display effect can be obtained. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following is a brief introduction to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0022] Figure 1 A schematic diagram of a process for displaying biological traces provided in an embodiment of the present application;

[0023] Figure 2 A schematic diagram of the atomization principle of the ultrasonic atomization device provided in the embodiment of the present application;

[0024] Figure 3 A schematic diagram of the process of biological traces remaining on the surface of an object to be displayed provided in an embodiment of the present application;

[0025] Figure 4 A schematic diagram of the visual fixing process of the blood and sweat handprint provided in an embodiment of the present application;

[0026] Figure 5 A schematic diagram of the display effect of a half-blood, half-sweat handprint obtained by the method for revealing biological traces provided in an embodiment of the present application;

[0027] Figure 6 Schematic diagram of the display effect of half-blood and half-sweat handprints on different objects to be displayed provided in the embodiments of the present application. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0029] At present, when the inventor uses traditional methods to test the potential biological traces remaining on non-permeable objects, the inventor takes latent blood fingerprints as an example. The inventor found that there is no blood around the latent blood fingerprints, but there are sweat components that cannot be detected by the naked eye left on the surface of the object. Therefore, these seemingly incomplete and incomplete blood fingerprints are actually complete fingerprints containing sweat and blood latents, but they exist in the form of half blood and half sweat fingerprints. Because the chemical composition of blood and sweat is different, the developer, development method and development procedure required are also different. And interference will occur during the detection process, affecting the development effect.

[0030] In order to solve the above technical problems, the embodiments of the present application provide the following technical ideas for solving the problems: first, Rhodamine 6G is used as the basic developer, and then an appropriate amount of hydrogen peroxide, methanol, acetic acid, ethyl acetate, glycerol and anhydrous ethanol are mixed to make a developer dye, and then with the help of ultrasonic atomization technology, the atomized developer dye liquid particles will generate highly active, oxidized chromophores under the catalysis of hemoglobin and heme, inducing sweat and blood to synchronously generate photoluminescence. Finally, under the excitation environment of blue-green light, a complete orange-red blood and sweat fluorescent handprint can be observed through orange goggles, and the display effect is good.

[0031] Embodiment 1:

[0032] In the present embodiment, the inventors found in the experimental process of preparing the developing dye that when multiple raw materials are mixed, the ratio and concentration have a great influence on the final development effect. Too low or too high a concentration can cause the particle size of the fluorescent molecules to change, disperse and accumulate, thereby weakening its specific binding ability to the papillary lines. Under the premise of ensuring the development effect, it is recommended to use a lower concentration and a small dose of the developing dye for biological traces.

[0033] In this embodiment, the raw materials of the developing dye include: rhodamine 6G, anhydrous ethanol and hydrogen peroxide;

[0034] The developing dye comprises the following raw materials when prepared in a volume of 100 ml: 0.025 g to 0.05 g of rhodamine 6G, 85 to 95 ml of anhydrous ethanol mixed with 5 to 15 ml of 30% hydrogen peroxide.

[0035] In this embodiment, Rhodamine 6G is an azo dye, and the fluorescent molecules absorb specific electromagnetic radiation and emit strong fluorescence, which has obvious advantages when processing reflective or colored substrates. Rhodamine 6G may have higher resolution and signal-to-noise ratio in handprint visualization.

[0036] In this embodiment, anhydrous ethanol is the carrier solvent of the developing dye. Anhydrous ethanol is more suitable as a blood fixative than 5-sulfosalicylic acid and is less harmful to humans and the environment.

[0037] In an optional embodiment of the present application, when preparing the developing dye, the order of adding rhodamine 6G, methanol, acetic acid, ethyl acetate and glycerol is set before the order of adding anhydrous ethanol.

[0038] Example 2

[0039] In this embodiment, the developing dye is prepared in a volume of 100 ml and includes the following raw materials: 0.025 g of rhodamine 6G, 85 ml of anhydrous ethanol mixed with 15 ml of 30% hydrogen peroxide.

[0040] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0041] Example 3

[0042] In this embodiment, the developing dye is prepared in a volume of 100 ml and includes the following raw materials: 0.05 g of rhodamine 6G, 85 ml of anhydrous ethanol mixed with 15 ml of 30% hydrogen peroxide.

[0043] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0044] Example 4

[0045] In this embodiment, the developing dye is prepared in a volume of 100 ml and includes the following raw materials: 0.025 g of rhodamine 6G, 95 ml of anhydrous ethanol mixed with 5 ml of 30% hydrogen peroxide.

[0046] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0047] Example 5

[0048] In this embodiment, the developing dye is prepared in a volume of 100 ml and includes the following raw materials: 0.05 g of rhodamine 6G, 95 ml of anhydrous ethanol mixed with 5 ml of 30% hydrogen peroxide.

[0049] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0050] Example 6

[0051] In this embodiment, the developing dye is prepared in a volume of 100 ml and includes the following raw materials: 0.05 g of rhodamine 6G, 90 ml of anhydrous ethanol mixed with 10 ml of 30% hydrogen peroxide.

[0052] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0053] Example 7

[0054] In this embodiment, the developing dye is prepared in a volume of 100 ml and includes the following raw materials: 0.025 g of rhodamine 6G, 90 ml of anhydrous ethanol mixed with 10 ml of 30% hydrogen peroxide.

[0055] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0056] Example 8

[0057] In this embodiment, the developing dye is prepared in a volume of 100 ml and includes the following raw materials: 0.04 g of rhodamine 6G, 90 ml of anhydrous ethanol mixed with 10 ml of 30% hydrogen peroxide.

[0058] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0059] Example 9

[0060] The difference from Example 1 is that the present application example provides a developing dye for biological traces, and the raw materials of the developing dye also include: methanol, acetic acid, ethyl acetate and glycerol;

[0061] The developing dye comprises the following raw materials when prepared in a volume of 100 ml: 0.025 g to 0.05 g of rhodamine 6G, 4.5 ml of methanol, 2 ml of acetic acid, 2.5 ml of ethyl acetate, 1 ml of glycerol, 85.5 ml of anhydrous ethanol and 4.5 ml of 30% hydrogen peroxide.

[0062] In this embodiment, please refer to Example 1 for the preparation method of the developing dye.

[0063] Example 10

[0064] An embodiment of the present application provides a developing stain for biological traces, which includes the following raw materials when prepared in a volume of 100 ml: 0.05 g of rhodamine 6G, 4.5 ml of methanol, 2 ml of acetic acid, 2.5 ml of ethyl acetate, 1 ml of glycerol, 85.5 ml of anhydrous ethanol and 4.5 ml of 30% hydrogen peroxide.

[0065] Embodiment 11

[0066] An embodiment of the present application provides a developing stain for biological traces, which includes the following raw materials when prepared in a volume of 100 ml: 0.25 g of rhodamine 6G, 4.5 ml of methanol, 2 ml of acetic acid, 2.5 ml of ethyl acetate, 1 ml of glycerol, 85.5 ml of anhydrous ethanol and 4.5 ml of 30% hydrogen peroxide.

[0067] Example 12

[0068] An embodiment of the present application provides a developing stain for biological traces, which includes the following raw materials when prepared in a volume of 100 ml: 0.35 g of rhodamine 6G, 4.5 ml of methanol, 2 ml of acetic acid, 2.5 ml of ethyl acetate, 1 ml of glycerol, 85.5 ml of anhydrous ethanol and 4.5 ml of 30% hydrogen peroxide.

[0069] In summary, the concentration range of the developing dye prepared according to the above embodiment can make the surface of the object to be displayed show clear and complete fingerprint lines. And different types of fingerprints have strong fluorescence characteristics. When using the developing dye for biological traces provided in the embodiment of the present application to perform biological trace inspection, there is no need to distinguish in advance whether the biological trace is a blood latent trace or a sweat latent trace, and a better display effect can be obtained.

[0070] Example 13 Figure 1 A schematic flow chart of a method for revealing biological traces provided in an embodiment of the present application.

[0071] Figure 3 A schematic diagram of the process of biological traces remaining on the surface of an object to be revealed provided in an embodiment of the present application.

[0072] like Figure 1 As shown, an embodiment of the present application provides a method for revealing biological traces. This embodiment takes a biological trace that is half blood and half sweat fingerprint as an example to describe the method steps.

[0073] like Figure 1 As shown, the method for revealing biological traces includes the following steps:

[0074] S101: Load the developing dye in Example 1 into an ultrasonic atomizing device, and place the nozzle of the ultrasonic atomizing device at a preset distance from the object to be developed, with the nozzle facing the object to be developed.

[0075] Figure 2 This is a schematic diagram of the atomization principle of the ultrasonic atomization device provided in an embodiment of the present application.

[0076] like Figure 2As shown, in this embodiment, the ultrasonic atomization device can be an ultrasonic atomizer, and the transducer of the ultrasonic atomizer can generate ultrasonic waves that propagate in the atomization medium, form surface tension waves, destroy the force between liquid molecules of the developer dye, and atomize the liquid.

[0077] In this embodiment, the nozzle type can be a straight nozzle or other nozzles with good atomization effect. The object to be displayed can be a non-permeable object, such as: a knife, a packaging bag, tin foil, aluminum foil, a tile, a copper sheet, a door handle, a table or a chair.

[0078] S102: Turn on the ultrasonic atomization device to atomize the developing dye from a liquid state into an aerosol state, so as to spray the developing dye in the aerosol state for a preset time onto the object to be developed.

[0079] In this embodiment, after the ultrasonic atomization device is turned on, it can atomize the developing dye from a liquid state into an aerosol state. The developing dye in the aerosol state is droplets, which will be sprayed on the surface of the object to be developed where potential biological traces may exist.

[0080] S103: Turn on the light source so that the potential biological traces on the surface of the object to be displayed can be observed by an observer wearing cut-off filter glasses.

[0081] In this embodiment, the light source may be an object that generates fluorescence, and the fluorescence can induce the chromophore to generate photoluminescence. The cut-off filter may be an orange filter, and the observer can observe the potential biological traces on the object to be displayed through the cut-off filter.

[0082] In summary, the method for developing biological traces provided in the embodiments of the present application does not require conventional complicated steps such as soaking, fixing, and rinsing. It is only necessary to first add the pre-prepared developing dye for biological traces into the ultrasonic atomization device, then point the nozzle toward the object to be displayed, and then turn on the ultrasonic atomization device. Then turn on the light source so that the observer can observe the potential biological traces on the surface of the object to be displayed through the cut-off filter glasses. It can be sprayed and displayed immediately, with high efficiency, and can simultaneously display the potential biological traces of blood and sweat, so as to obtain more complete biological traces and better display effects.

[0083] Figure 4 Schematic diagram of the visual fixation process of the blood and sweat fingerprint provided in an embodiment of the present application.

[0084] Based on the above embodiments, Figure 4 As shown, in an optional embodiment of the present application, after the light source is turned on in step S103, the process further includes:

[0085] S104: Turn on an image acquisition device, wherein a cutoff filter is installed on a camera of the image acquisition device.

[0086] In this embodiment, the image acquisition device may be a device capable of capturing images or video data, such as a video camera, a still camera, or a recorder. A cutoff filter is also installed on the camera of the image acquisition device, so that the bloody handprint can be captured and fixed later as needed.

[0087] S105: Using an image acquisition device, photograph potential biological traces on the surface of the object to be detected to obtain target trace image data.

[0088] In this embodiment, the target trace image data refers to a picture or video containing potential biological traces. Obtaining the target trace image data can facilitate the fixation and preservation of evidence.

[0089] In an optional embodiment of the present application, potential biological traces on the surface of the object to be displayed are photographed by an image acquisition device, including: adjusting the shooting focal length of the image acquisition device by the image acquisition device to photograph the potential biological traces on the surface of the object to be displayed.

[0090] In this embodiment, adjusting the shooting focal length can enable the image acquisition device to acquire a clearer image. For example, adjusting the camera focal length of a camera to focus the camera.

[0091] like Figure 4 As shown, in this embodiment, after the potential biological traces are formed on the object, an ultrasonic atomization device is used to adsorb and combine the aerosol-state developing dye with the residual blood, residual oil and sweat. Then, under the illumination of a light source, the complete biological traces can be viewed by wearing goggles equipped with a cutoff filter. Alternatively, a camera with a filter installed at the lens can be used to photograph and fix the biological traces.

[0092] Figure 5 A schematic diagram of the display effect of a half-blood, half-sweat handprint obtained by the method for revealing biological traces provided in an embodiment of the present application.

[0093] like Figure 5 As shown, for the half-blood and half-sweat handprint, the detailed features in the figure include: 1 small bridge; 2, branch 1; 3, starting point; 4, branch 2; 5, first joint; 6, small dot; 7, short stick; 8, second joint. It can be seen that the biological trace display method provided in the embodiment of the present application can make the biological trace on the object to be displayed have a high degree of integrity.

[0094] In an optional embodiment of the present application, the spray rate of the nozzle is 0.15ml / min to 0.30ml / min, the ultrasonic operating frequency of the ultrasonic atomization device is 1MHz to 3MHz; the preset distance is 10cm to 15cm, the particle size of the developing dye in the aerosol state is 3.0μm±25%, and the preset time is 5s to 10s.

[0095] Example 1: The spray rate of the nozzle is 0.15 ml / min, the ultrasonic working frequency of the ultrasonic atomization device is 1 MHz, the preset distance is 10 cm, the particle size of the developing dye in the aerosol state is 2.25 μm, and the preset time is 5 seconds.

[0096] Example 2: The spray rate of the nozzle is 0.3 ml / min, the ultrasonic working frequency of the ultrasonic atomization device is 3 MHz, the preset distance is 15 cm, the particle size of the developing dye in the aerosol state is 3.75 μm, and the preset time is 10 seconds.

[0097] Example 3: The spray rate of the nozzle is 0.2 ml / min, the ultrasonic working frequency of the ultrasonic atomization device is 2 MHz, the preset distance is 12 cm, the particle size of the developing dye in the aerosol state is 3 μm, and the preset time is 8 seconds.

[0098] In an optional embodiment of the present application, an ultrasonic atomization device is turned on to atomize the developer dye from a liquid state into an aerosol state, so as to spray the developer dye in an aerosol state for a preset time length onto the object to be developed, including: turning on the ultrasonic atomization device, controlling the operating frequency of the ultrasonic atomization device, and adjusting the average particle size of the developer dye in an aerosol state, so as to spray the developer dye in an aerosol state for a preset time length onto the object to be developed.

[0099] In this embodiment, the particle size of the developer in aerosol state can be adjusted by controlling the working frequency of the ultrasonic atomization device, thereby achieving precise control of the particle size, so that the developer in aerosol state can spread faster and adhere to the surface of the object to be developed.

[0100] Specifically, in an optional embodiment of the present application, the operating frequency of the ultrasonic atomization device is 1.67 MHz, and the average particle size of the developing dye in the aerosol state is 2.582 μm.

[0101] In an optional embodiment of the present application, the light source is a dual-band light source, a multi-band light source or a laser.

[0102] In an optional embodiment of the present application, the fluorescence wavelength generated by the light source is 450 to 590 nm.

[0103] Specifically, in an optional embodiment of the present application, the fluorescence generated by the light source is 530nm green light.

[0104] In an optional embodiment of the present application, the fluorescence wavelength generated by the light source is 450nm.

[0105] In an optional embodiment of the present application, the fluorescence wavelength generated by the light source is 590 nm.

[0106] In an optional embodiment of the present application, in order to facilitate operation, the ultrasonic atomization device, the light source and the image acquisition device are integrated into a developer, and the developer also includes a power supply device and a control device. The power supply device is electrically connected to the ultrasonic atomization device, the light source, the image acquisition device and the control device, and the control device is communicatively connected to the ultrasonic atomization device, the light source and the image acquisition device.

[0107] In this embodiment, the ultrasonic atomization device, the light source and the image acquisition device can be used to implement the visualization method in Embodiment 2 after being powered on.

[0108] Figure 6 Schematic diagram of the display effect of half-blood and half-sweat handprints on different objects to be displayed provided in the embodiments of the present application.

[0109] like Figure 6 As shown, these objects to be displayed include a. kitchen knife, b. tin foil, c. aluminum foil, d. blue tile, e. copper sheet, f. black tile, g. black packaging bag, and the biological traces on different objects have different display effects, but each object to be displayed can show a relatively complete and stable half-blood and half-handprint. To illustrate the technical solution of this application, rather than to limit it; although the application is described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that: it is still possible to modify the technical solutions recorded in the aforementioned embodiments, or to replace some or all of the technical features therein; and these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of each embodiment of this application.

Claims

1. A developing dye for biological traces, characterized in that: The developing dye is composed of rhodamine 6G, anhydrous ethanol and hydrogen peroxide; The developing dye comprises the following raw materials when prepared in a volume of 100 ml: 0.025 g to 0.05 g of rhodamine 6G, 85 to 95 ml of anhydrous ethanol mixed with 5 to 15 ml of 30% hydrogen peroxide; the developing dye is used to achieve the simultaneous visualization of blood and sweat components in biological traces.

2. A developing dye for biological traces, characterized in that: The developing dye is composed of rhodamine 6G, anhydrous ethanol, hydrogen peroxide, methanol, acetic acid, ethyl acetate and glycerol; The developing dye comprises the following raw materials when prepared in a volume of 100 ml: 0.025 g to 0.05 g of rhodamine 6G, 4.5 ml of methanol, 2 ml of acetic acid, 2.5 ml of ethyl acetate, 1 ml of propylene glycol, 85.5 ml of anhydrous ethanol and 4.5 ml of 30% hydrogen peroxide.

3. A method for revealing biological traces, characterized in that: The steps include: The developing dye as claimed in claim 1 is loaded into an ultrasonic atomizing device, and the nozzle of the ultrasonic atomizing device is placed at a preset distance from the object to be developed, and the nozzle faces the object to be developed; Turning on the ultrasonic atomization device to atomize the developing dye from a liquid state into an aerosol state, so as to spray the developing dye in the aerosol state for a preset time onto the object to be developed; The light source is turned on, so that the potential biological traces on the surface of the object to be displayed can be observed by an observer wearing cut-off filter glasses.

4. The method according to claim 3, characterized in that After turning on the light source, it also includes: Turning on an image acquisition device, wherein a cutoff filter is installed on a camera of the image acquisition device; The image acquisition device is used to photograph the potential biological traces on the surface of the object to be displayed to obtain target trace image data.

5. The method according to claim 4, characterized in that The method of photographing the potential biological traces on the surface of the object to be displayed by the image acquisition device includes: The image acquisition device is used to adjust the shooting focal length of the image acquisition device to shoot potential biological traces on the surface of the object to be displayed.

6. The method according to claim 4, characterized in that The spray rate of the nozzle is 0.15ml / min to 0.30ml / min, the ultrasonic working frequency of the ultrasonic atomization device is 1MHz to 3MHz; the preset distance is 10cm to 15cm, the particle size of the developing dye in the aerosol state is 3.0μm±25%, and the preset time is 5s to 10s.

7. The method according to claim 6, characterized in that The ultrasonic atomization device is turned on to atomize the developing dye from a liquid state into an aerosol state, so as to spray the developing dye in an aerosol state for a preset time onto the object to be developed, comprising: The ultrasonic atomization device is turned on, the working frequency of the ultrasonic atomization device is controlled, and the average particle size of the aerosol-state developing dye is adjusted to spray the aerosol-state developing dye for a preset time onto the object to be developed.

8. The method according to claim 7, characterized in that The working frequency of the ultrasonic atomization device is 1.67 MHz, and the average particle size of the developing dye in the aerosol state is 2.582 μm.

9. The method according to any one of claims 3 to 8, characterized in that The light source generates a fluorescent light with a wavelength of 450 to 530 nm.

10. The method according to claim 5, characterized in that The ultrasonic atomization device, the light source and the image acquisition device are integrated into a developer, which also includes a power supply device and a control device. The power supply device is electrically connected to the ultrasonic atomization device, the light source, the image acquisition device and the control device, and the control device is communicatively connected to the ultrasonic atomization device, the light source and the image acquisition device.

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