Method and application for promoting the growth rate of acclimatization of tissue culture seedlings of Panax ginseng

Abstract

The present invention relates to the technical field of Chinese medicinal material cultivation, in particular to a method and application for promoting the acclimation growth rate of ginseng vine vine tissue culture seedlings, wherein the nutrient solution composition comprises: a nutrient solution A for soaking the tissue culture seedlings, a nutrient solution B for foliar spraying after 7 days of acclimation, a nutrient solution C for foliar spraying after 15 days of acclimation, and a nutrient solution D for root irrigation after 30 days of acclimation. The present invention can shorten the acclimation time from the original 10 to 12 months to 5 to 6 months, greatly reduce the management cost, and improve the annual production efficiency and land utilization rate of ginseng vine vine.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicinal material cultivation, in particular to a method for promoting the acclimation growth rate of Panax ginseng vine tissue culture seedlings and an application thereof. Background Art

[0002] Tylophora kerrii Craib, also known as wild ginseng, wild cow seven, and mountain bean root, is a small climbing shrub of the genus Tylophora in the Apocynaceae family. Its root is used as medicine, which has the effects of clearing the liver and improving eyesight, promoting qi and relieving pain. It is mainly used for blurred vision and abdominal distension and pain. Modern Chinese medicine research shows that Tylophorine, Tylocrebrine, and Tylophorinine isolated from Tylophora plants have significant anti-cancer activity, and are a good drug resource with high development and utilization value.

[0003] There are very few existing medicinal plant resources of Panax ginseng, and the medical demand is increasing. It has become a trend to use tissue culture technology to quickly propagate Panax ginseng. The publication number is: CN105532453A, and the name is: A two-step seedling tissue culture method for Panax ginseng; the publication number is: CN116569844A, and the name is: A tissue culture rapid propagation method for inducing clustered buds using Panax ginseng stem segments. The above method can achieve rapid propagation of Panax ginseng, and the survival rate of domestication and transplantation is high. However, the tissue culture seedlings of Panax ginseng grow very slowly during domestication. It takes 6 to 7 months to initially start growing fleshy roots, and 10 to 12 months before they can be transplanted. The domestication period is long and the domestication cost is high.

[0004] Therefore, it is very necessary to develop a method to promote the acclimatization growth rate of Panax ginseng vine tissue culture seedlings. Summary of the invention

[0005] The purpose of the present invention is to provide a method and application for promoting the acclimation growth rate of Panax ginseng vine tissue culture seedlings.

[0006] To achieve the above object, the technical solution adopted by the present invention is: a nutrient solution composition for promoting the acclimation growth rate of tissue culture seedlings of Panax ginseng, the nutrient solution composition comprising: nutrient solution A for soaking tissue culture seedlings, nutrient solution B for foliar spraying after 7 days of acclimation, nutrient solution C for foliar spraying after 15 days of acclimation, and nutrient solution D for root irrigation after 30 days of acclimation;

[0007] The nutrient solution A component is: IBA 3.0-4.0 mg / L+NAA 2.0-3.0 mg / L+BR 0.05-0.08 mg / L+50% benomyl powder 0.5-1.0 mg / L;

[0008] The nutrient solution B component is: Hoagland nutrient solution + IBA 5.0-6.0 mg / L;

[0009] The nutrient solution component C is: Hoagland nutrient solution + IBA 0.5-1.0 mg / L + BR 0.02-0.04 mg / L + KT10.0-12.0 mg / L;

[0010] The nutrient solution D component is: Hoagland nutrient solution + KT 30-40 mg / L + CCC 80-100 mg / L + potassium dihydrogen phosphate 100-120 mg / L.

[0011] Further:

[0012] The nutrient solution A component is: IBA 3.5mg / L+NAA 2.5mg / L+BR 0.07mg / L+50% benomyl powder 0.7mg / L;

[0013] The nutrient solution B component is: Hoagland nutrient solution + IBA 5.5 mg / L;

[0014] The nutrient solution component C is: Hoagland nutrient solution + IBA 0.7mg / L + BR 0.03mg / L + KT 11.0mg / L;

[0015] The nutrient solution D component is: Hoagland nutrient solution + KT 35 mg / L + CCC 90 mg / L + potassium dihydrogen phosphate 110 mg / L.

[0016] The second object of the present invention is to provide a method for promoting the growth rate of acclimatization of Panax ginseng vine tissue culture seedlings by using the nutrient solution composition of the present invention, the steps comprising:

[0017] (1) After the tissue culture seedlings are taken out of the bottle and washed, the whole plant is soaked in nutrient solution A for 1 to 2 minutes and then transplanted into the acclimation medium;

[0018] (2) After acclimation of the tissue culture seedlings for 7 days, spray nutrient solution B on the leaves at a rate of 30-40 kg / mu;

[0019] (3) After 15 days of acclimatization of the tissue culture seedlings, spray nutrient solution C on the leaves at a rate of 30-40 kg / mu;

[0020] (4) After 30 days of acclimatization, irrigate the roots with nutrient solution D at a rate of 200-250 kg / mu;

[0021] (5) After that, irrigate the roots with nutrient solution D every 10 to 15 days at a rate of 200 to 250 kg / mu.

[0022] Further, the steps include:

[0023] (1) After the tissue culture seedlings are taken out of the bottle and washed, the whole plant is soaked in nutrient solution A for 1.5 minutes and then transplanted into the acclimation medium;

[0024] (2) After acclimation of the tissue culture seedlings for 7 days, spray nutrient solution B on the leaves at a rate of 35 kg / mu;

[0025] (3) After 15 days of acclimatization of the tissue culture seedlings, spray nutrient solution C on the leaves at a rate of 35 kg / mu;

[0026] (4) After 30 days of acclimatization, the roots of the seedlings were irrigated with nutrient solution D at a rate of 225 kg / mu;

[0027] (5) Every 13 days, irrigate the roots with nutrient solution D at a rate of 225 kg / mu.

[0028] Furthermore, the domestication matrix is ​​humus and perlite, and the mass ratio of the two is 4 to 5:1.

[0029] The beneficial technical effects of the present invention are:

[0030] 1. In the present invention, at the beginning of domestication, a mixed nutrient solution consisting of IBA, NAA and BR is used to soak the tissue culture seedlings, and a mixed nutrient solution consisting of Hoagland nutrient solution and IBA is used for foliar spraying 7 days after planting, and a mixed nutrient solution consisting of Hoagland nutrient solution, IBA, BR and KT is used for foliar spraying 15 days after planting. This can promote the rapid elongation and growth of adventitious roots during the domestication of Panax ginseng baby vine, and can also promote the tillering of aboveground buds to a certain extent, thereby producing new leaves and new branches, which can accelerate the transformation from heterotrophy to autotrophy.

[0031] 2. After 30 days of planting, the adventitious roots of the present invention have been generated and elongated in large quantities, and new stems and leaves have begun to be generated in large quantities, which has completely transformed from anomaly to autotrophy. At this time, a mixed nutrient solution consisting of Hogland nutrient solution, KT, CCC, and potassium dihydrogen phosphate is used for root irrigation. After that, the mixed nutrient solution is used for root irrigation every 10 to 15 days. On the one hand, it can effectively promote the elongation and thickening of the adventitious roots, and finally promote fleshiness. On the other hand, it can make the aboveground stems and leaves grow new, and at the same time, it can make the stems stronger and the leaves thicker and greener.

[0032] 3. The present invention can shorten the domestication time from the original 10 to 12 months to 5 to 6 months, greatly reducing the management cost and improving the annual production efficiency and land utilization rate of Panax ginseng. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0034] Figure 1 This is a graph showing the results of acclimatization for one month in Example 4 of the present invention.

[0035] Figure 2 It is the result diagram of acclimatization for 3 months of Example 4 of the present invention.

[0036] Figure 3 It is the result diagram of acclimation for 5 months of Example 4 of the present invention. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Example 1

[0039] A nutrient solution composition for promoting the acclimation growth rate of tissue culture seedlings of Panax ginseng, the nutrient solution composition comprising: nutrient solution A for soaking tissue culture seedlings, nutrient solution B for foliar spraying after 7 days of acclimation, nutrient solution C for foliar spraying after 15 days of acclimation, and nutrient solution D for root irrigation after 30 days of acclimation;

[0040] Component A of nutrient solution is: IBA 3.0mg / L+NAA 2.0mg / L+BR 0.05mg / L+50% benomyl powder 0.5mg / L;

[0041] Nutrient solution B component is: Hoagland nutrient solution + IBA 5.0mg / L;

[0042] Component C of nutrient solution is: Hoagland nutrient solution + IBA 0.5mg / L + BR 0.02mg / L + KT 10.0mg / L;

[0043] Component D of nutrient solution is: Hoagland nutrient solution + KT 30mg / L + CCC 80mg / L + potassium dihydrogen phosphate 100mg / L.

[0044] Example 2

[0045] A nutrient solution composition for promoting the acclimation growth rate of tissue culture seedlings of Panax ginseng, the nutrient solution composition comprising: nutrient solution A for soaking tissue culture seedlings, nutrient solution B for foliar spraying after 7 days of acclimation, nutrient solution C for foliar spraying after 15 days of acclimation, and nutrient solution D for root irrigation after 30 days of acclimation;

[0046] Component A of nutrient solution is: IBA 4.0mg / L+NAA 3.0mg / L+BR 0.08mg / L+50% benomyl powder 1.0mg / L;

[0047] Nutrient solution B component is: Hoagland nutrient solution + IBA 6.0mg / L;

[0048] Nutrient solution C component is: Hoagland nutrient solution + IBA 1.0mg / L + BR 0.04mg / L + KT 12.0mg / L;

[0049] Component D of nutrient solution is: Hoagland nutrient solution + KT 40mg / L + CCC 100mg / L + potassium dihydrogen phosphate 120mg / L.

[0050] Example 3

[0051] A nutrient solution composition for promoting the acclimation growth rate of tissue culture seedlings of Panax ginseng, the nutrient solution composition comprising: nutrient solution A for soaking tissue culture seedlings, nutrient solution B for foliar spraying after 7 days of acclimation, nutrient solution C for foliar spraying after 15 days of acclimation, and nutrient solution D for root irrigation after 30 days of acclimation;

[0052] Component A of nutrient solution is: IBA 3.5mg / L+NAA 2.5mg / L+BR 0.07mg / L+50% benomyl powder 0.7mg / L;

[0053] Nutrient solution B component is: Hoagland nutrient solution + IBA 5.5mg / L;

[0054] Component C of nutrient solution is: Hoagland nutrient solution + IBA 0.7mg / L + BR 0.03mg / L + KT 11.0mg / L;

[0055] Component D of nutrient solution is: Hoagland nutrient solution + KT 35mg / L + CCC 90mg / L + potassium dihydrogen phosphate 110mg / L.

[0056] Example 4

[0057] A method for promoting the growth rate of acclimatized Panax ginseng vine tissue culture seedlings by using a nutrient solution composition, wherein the specific formula of the nutrient solution composition is the formula of Example 1, and the steps include:

[0058] (1) After the tissue culture seedlings are taken out of the bottle and washed, the whole plant is soaked in nutrient solution A for 1 min and then transplanted into the acclimation medium. The acclimation medium is humus and perlite, with a mass ratio of 4:1.

[0059] (2) After 7 days of acclimatization, spray nutrient solution B on the leaves at a rate of 30 kg / mu.

[0060] (3) After 15 days of acclimatization, spray nutrient solution C on the leaves at a rate of 30 kg / mu.

[0061] (4) After 30 days of acclimatization, the seedlings were irrigated with nutrient solution D at a rate of 200 kg / mu. The acclimatization results are shown in the figure below. Figure 1 As shown in the figure, it can be seen that the root system of the tissue culture seedlings is well developed.

[0062] (5) Every 10 days, irrigate the roots with nutrient solution D at a rate of 200 kg / mu.

[0063] The seedlings were acclimated for 3 months and the results were as follows Figure 2 As shown, the roots have begun to become fleshy; after 5 months of acclimatization, the results are as follows Figure 3 As shown, the seedlings grew vigorously.

[0064] Example 5

[0065] A method for promoting the acclimation growth rate of Panax ginseng vine tissue culture seedlings by using the nutrient solution composition of the present invention, wherein the specific formula of the nutrient solution composition is the formula of Example 2, and the steps include:

[0066] (1) After the tissue culture seedlings are taken out of the bottle and washed, the whole plant is soaked in nutrient solution A for 2 minutes and then transplanted into the acclimation medium. The acclimation medium is: humus soil and perlite, and the mass ratio of the two is: 5:1.

[0067] (2) After 7 days of acclimatization, spray nutrient solution B on the leaves at a rate of 40 kg / mu.

[0068] (3) After 15 days of acclimatization, spray nutrient solution C on the leaves at a rate of 40 kg / mu.

[0069] (4) After 30 days of acclimatization, the roots of the seedlings were irrigated with nutrient solution D at a rate of 250 kg / mu.

[0070] (5) After that, irrigate the roots with nutrient solution D every 10 to 15 days at a rate of 250 kg / mu.

[0071] Example 6

[0072] A method for promoting the acclimation growth rate of Panax ginseng vine tissue culture seedlings by using the nutrient solution composition of the present invention, wherein the specific formula of the nutrient solution composition is the formula of Example 3, and the steps include:

[0073] (1) After the tissue culture seedlings are taken out of the bottle and washed, the whole plant is soaked in nutrient solution A for 1.5 minutes and then transplanted into the acclimation medium. The acclimation medium is: humus soil and perlite, and the mass ratio of the two is: 4.5:1.

[0074] (2) After 7 days of acclimatization, spray nutrient solution B on the leaves at a rate of 35 kg / mu.

[0075] (3) After 15 days of acclimatization, spray nutrient solution C on the leaves at a rate of 35 kg / mu.

[0076] (4) After 30 days of acclimatization, the roots of the seedlings were irrigated with nutrient solution D at a rate of 225 kg / mu.

[0077] (5) After that, irrigate the roots with nutrient solution D every 10 to 15 days at a rate of 225 kg / mu.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. Any modification or partial replacement that does not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A nutrient solution composition for promoting the acclimatization growth rate of Panax ginseng vine tissue culture seedlings, characterized in that: The nutrient solution composition comprises: nutrient solution A for soaking tissue culture seedlings, nutrient solution B for foliar spraying after 7 days of acclimation, nutrient solution C for foliar spraying after 15 days of acclimation, and nutrient solution D for root irrigation after 30 days of acclimation; The nutrient solution A component is: IBA 3.0-4.0 mg / L+NAA 2.0-3.0 mg / L+BR 0.05-0.08 mg / L+50% benomyl powder 0.5-1.0 mg / L; The nutrient solution B component is: Hoagland nutrient solution + IBA 5.0-6.0 mg / L; The nutrient solution component C is: Hoagland nutrient solution + IBA 0.5-1.0 mg / L + BR 0.02-0.04 mg / L + KT 10.0-12.0 mg / L; The nutrient solution D component is: Hoagland nutrient solution + KT 30-40 mg / L + CCC 80-100 mg / L + potassium dihydrogen phosphate 100-120 mg / L.

2. The nutrient solution composition according to claim 1, characterized in that: The nutrient solution A component is: IBA 3.5mg / L+NAA 2.5mg / L+BR 0.07mg / L+50% benomyl powder 0.7mg / L; The nutrient solution B component is: Hoagland nutrient solution + IBA 5.5 mg / L; The nutrient solution component C is: Hoagland nutrient solution + IBA 0.7mg / L + BR 0.03mg / L + KT 11.0mg / L; The nutrient solution D component is: Hoagland nutrient solution + KT 35 mg / L + CCC 90 mg / L + potassium dihydrogen phosphate 110 mg / L.

3. A method for promoting the growth rate of acclimatized Panax ginseng vine tissue culture seedlings by using the nutrient solution composition according to claim 1 or 2, characterized in that the steps include: (1) After the tissue culture seedlings are taken out of the bottle and washed, the whole plant is soaked in nutrient solution A for 1 to 2 minutes and then transplanted into the acclimation medium; (2) After acclimation of the tissue culture seedlings for 7 days, spray nutrient solution B on the leaves at a rate of 30-40 kg / mu; (3) After 15 days of acclimatization of the tissue culture seedlings, spray nutrient solution C on the leaves at a rate of 30-40 kg / mu; (4) After 30 days of acclimatization, irrigate the roots with nutrient solution D at a rate of 200-250 kg / mu; (5) After that, irrigate the roots with nutrient solution D every 10 to 15 days at a rate of 200 to 250 kg / mu.

4. The method according to claim 3, characterized in that the steps include: (1) After the tissue culture seedlings are taken out of the bottle and washed, the whole plant is soaked in nutrient solution A for 1.5 minutes and then transplanted into the acclimation medium; (2) After acclimation of the tissue culture seedlings for 7 days, spray nutrient solution B on the leaves at a rate of 35 kg / mu; (3) After 15 days of acclimatization of the tissue culture seedlings, spray nutrient solution C on the leaves at a rate of 35 kg / mu; (4) After 30 days of acclimatization, the roots of the seedlings were irrigated with nutrient solution D at a rate of 225 kg / mu; (5) Every 13 days, irrigate the roots with nutrient solution D at a rate of 225 kg / mu.

5. The method according to claim 3 or 4, characterized in that: The domestication matrix includes humus and perlite, and the mass ratio of the two is 4-5:

1.

6. Application of the method according to claim 3 or 4 in promoting the acclimatization growth rate of Panax ginseng vine tissue culture seedlings.

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