A slide dyeing integrated workstation and a slide dyeing method thereof

Abstract

The application provides a slice preparation and dyeing integrated workstation, which comprises a feeding area and a discharging area, and a first multi-axis robot, a second multi-axis robot and a dyeing pool are sequentially arranged between the feeding area and the discharging area. The centrifugal pipetting integrated device comprises a bottom frame, a first rotating disc and a second rotating disc are coaxially arranged on the bottom frame and can rotate, a plurality of sampling bottle placing holes are circumferentially arranged on the first rotating disc, the sampling bottle placing holes are used for placing sampling bottles, a plurality of slice preparation bin placing grooves are circumferentially arranged on the second rotating disc, the slice preparation bin placing grooves are used for placing slice preparation bins, a multi-axis moving mechanism is further arranged on the bottom frame, the multi-axis moving mechanism is provided with a pipetting device, the pipetting device is used for transferring liquid in the sampling bottles and the slice preparation bins, the first multi-axis robot is used for feeding the centrifugal pipetting integrated device from the feeding area, and the second multi-axis robot is used for clamping the dyed glass slides after centrifugation. The problem of loose distribution of centrifugal stations and pipetting stations in traditional slice preparation machines is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cell detection, in particular to a slice preparation and staining integrated workstation and a slice preparation and staining method thereof. BACKGROUND

[0002] Liquid-based thin-layer cell preparation is a kind of cervical cancer screening technology that is currently used more frequently. It is to put the cervical exfoliative cells into a sampling bottle containing cell preservative solution, and to prepare thin-layer cells by centrifugation and other methods, and then to fix the thin-layer cells on a glass slide, and to complete the process by staining and mounting. This cervical cancer screening method has a series of advantages such as favorable price, accurate results, and fast detection time.

[0003] In the traditional slice preparation station, pipetting and centrifugation belong to two different positions, and are usually arranged separately. Since the centrifugation process occupies a relatively long tact time, the centrifugation mechanism needs to be set as a rotating disc type, and the slice preparation bin needs to be centrifuged in batches to share the tact time, so the centrifugation mechanism itself needs a relatively large area, and in addition to the pipetting position and the sampling bottle buffer position upstream of the pipetting position, the overall equipment occupies a large area, and the structure is not compact enough. SUMMARY

[0004] The present application provides a slice preparation and staining integrated workstation and a slice preparation and staining method thereof, which solves the problem of loose distribution of the centrifugation position and the pipetting position of the traditional slice preparation machine, resulting in a large area occupied by the slice preparation machine and an insufficiently compact structure.

[0005] To solve the above technical problems, the technical scheme adopted by the present application is as follows: a slice preparation and staining integrated workstation, comprising a feeding area and a discharging area, a first multi-axis robot, a second multi-axis robot and a staining pool are sequentially arranged between the feeding area and the discharging area, a centrifugation and pipetting integrated device comprises a bottom frame, a first rotating disc and a second rotating disc are coaxially arranged on the bottom frame and can rotate, the first rotating disc is provided with a plurality of sampling bottle placing holes in the circumferential direction, the sampling bottle placing holes are used for placing sampling bottles, the second rotating disc is provided with a plurality of slice preparation bin placing grooves in the circumferential direction, the slice preparation bin placing grooves are used for placing slice preparation bins, a multi-axis moving mechanism is further arranged on the bottom frame, the multi-axis moving mechanism is provided with a pipetting device, the pipetting device is used for transferring the liquid in the sampling bottles and the slice preparation bins, the first multi-axis robot is used for feeding the centrifugation and pipetting integrated device from the feeding area, and the second multi-axis robot is used for clamping the glass slides after centrifugation for staining.

[0006] In the preferred scheme, a third rotating disc coaxially arranged with the first rotating disc and the second rotating disc is further included, the third rotating disc is arranged above the first rotating disc and the second rotating disc, a plurality of pipetting tube resting holes are arranged on the third rotating disc in the circumferential direction, and the pipetting tube resting holes are used for temporarily storing pipetting tubes.

[0007] In the preferred solution, the bottom frame is provided with a multi-axis moving mechanism, the multi-axis moving mechanism comprises a first linear module, a second linear module is arranged on the sliding plate of the first linear module, a liquid transfer device is connected to the sliding plate of the second linear module, the first linear module and the second linear module are arranged vertically, and a waste barrel is further arranged on one side of the bottom frame. The first linear module drives the liquid transfer device to move above the waste barrel to discharge waste liquid.

[0008] In the preferred solution, a stop shoulder is arranged outside the open end of the pipette, the stop shoulder is clamped in the pipette resting hole, a side slot is arranged on the outer edge side of the third rotating disc near the pipette resting hole, a spring plunger is arranged on the third rotating disc, the spring plunger comprises a retractable ball, and the ball abuts against the outer wall of the pipette.

[0009] In the preferred solution, the spring plunger comprises a plunger barrel, a front plug and a rear stop block are arranged in the plunger barrel, the front plug is slidably sleeved with the inner wall of the plunger barrel, one end of the front plug abuts against the ball, and a second spring is arranged between the front plug and the rear stop block.

[0010] In the preferred solution, a rotatable tablet bin resting plate is arranged at the tablet bin placing groove, the liquid transfer device is provided with a connecting hanger, a magnetic block part is arranged at the lower end of the connecting hanger, the magnetic block part attracts one end of the tablet bin resting plate to make the tablet bin inclined, and the pipette is inserted into the liquid injection cylinder of the tablet bin to suck out waste liquid.

[0011] In the preferred solution, the connecting hanger comprises a fork-shaped frame, the open end of the fork-shaped frame is connected with the liquid transfer device, a hanger rod is arranged at the lower end of the fork-shaped frame, a horizontal plate is connected to the lower end of the hanger rod, and the magnetic block part is arranged at the lower end of the horizontal plate on both sides.

[0012] In the preferred solution, the liquid transfer device comprises a base block, a pipe clamp is arranged at the lower end of the base block, the pipe clamp comprises a columnar cylinder, the outer wall diameter of the columnar cylinder is smaller than the inner diameter of the pipette, one end of the inner cavity of the columnar cylinder is closed, an opening is arranged at one end of the columnar cylinder close to the pipe clamp, a plurality of retractable tongues are arranged at the opening end of the columnar cylinder in the circumferential direction, a wedge surface is arranged on the side close to the inside of the retractable tongue, a tapered plug is arranged in the columnar cylinder, a first spring is arranged at one end of the tapered plug, the first spring abuts against the closed end of the columnar cylinder, the wedge surface of each retractable tongue abuts against the tapered surface of the tapered plug, an annular arc groove is arranged on the outer wall of the retractable tongue, a flange is arranged on the inner wall of the upper end port of the pipette, the annular arc groove is clamped on the flange, a filling block is arranged between the retractable tongues in the columnar cylinder, a second air channel is arranged in the base block, and one end of the second air channel is communicated with the opening end of the columnar cylinder.

[0013] In the preferred solution, a sealing block is arranged at the lower end of the base block, the sealing block blocks the upper end port of the pipette, a second air hole is arranged in the center of the sealing block, the second air hole is communicated with the second air channel, a plurality of first air holes are arranged on the sealing block in the axial direction, an air circulation ring groove is arranged in the base block, each first air hole is communicated through the air circulation ring groove, a first air channel is further arranged in the base block, and one end of the first air channel is communicated with the air circulation ring groove.

[0014] In the preferred solution,

[0015] The first multi-axis robot transfers the sample bottle in the sample bottle preparation tray to the sample bottle placement hole, transfers the empty slice preparation bin in the slice preparation bin preparation tray to the slice preparation bin placement groove, and transfers the empty pipette in the pipette preparation tray to the pipette resting hole.

[0016] In the slice preparation stage, the first rotating disc, the second rotating disc and the third rotating disc rotate to align the slice preparation bin, the sample bottle and the pipette on the radial line, the pipette is loaded from the pipette resting hole on the radial line, transferred above the sample bottle, lowered and aspirated the liquid in the sample bottle, and transferred into the liquid injection cylinder of the slice preparation bin on the corresponding radial line, then the pipette is placed back in the pipette resting hole, the second rotating motor drives the second rotating disc to rotate at high speed, the cells are deposited on the glass slide, and the slice preparation is completed.

[0017] In the cleaning stage, the first rotating disc, the second rotating disc and the third rotating disc rotate again to align the slice preparation bin, the sample bottle and the pipette according to the original position on the radial line, the pipette is loaded from the pipette resting hole on the radial line, the waste liquid in the slice preparation bin is aspirated and discharged into the waste barrel, the liquid outlet injects cleaning liquid, then the waste liquid is aspirated and discharged into the waste barrel again, then the pipette is discarded into the waste barrel, since each pipette corresponds to a slice preparation bin and a sample bottle, and is not cross-utilized, there is no sample contamination.

[0018] In the staining stage, the second multi-axis robot clamps the liquid injection cylinder of the slice preparation bin and removes it and places it in the recycling channel, then clamps the glass slide and transfers it to the slide rack on one side of the staining pool, after the slide rack is full, the second multi-axis robot clamps the slide rack and places it in the staining pool for soaking and staining, and places the stained slide rack at the slide unloading position.

[0019] The beneficial effects of the present application are: the sampling bottle placement station is arranged concentrically with the centrifugal turntable, the planar position space is saved, the structure of the tablet machine is more compact, and the miniaturization of the equipment is beneficial; the transfer mechanism is arranged above the centrifugal turntable, the pipette is used to transfer the cell sample in the sampling bottle to the tablet bin, and the waste liquid after centrifugation can also be sucked out by the pipette, so that the tablet bin overturning mechanism is omitted, and the cost is saved; a third turntable for temporarily placing the pipette is added, the pipette is temporarily placed on the third turntable after the first transfer from the sampling bottle to the tablet bin, and is left for subsequent waste liquid cleaning, the use amount of the pipette can be reduced by half, and the consumable cost is greatly reduced; the pipette is controlled by the double air path to load, unload, transfer and discharge the liquid, and the operation is simple, convenient and fast; the pipette device necessary for tabletting is used as a waste liquid cleaning device, an overturning mechanism does not need to be additionally increased, the cost is low, and no additional space is occupied; the rear end of the tablet bin resting plate is adsorbed by the magnetic block part of the connecting hanging bracket which is lifted synchronously with the pipette device, so that the rear end is lifted, the waste liquid at the bottom is more concentrated, and the waste liquid cleaning is more thorough. BRIEF DESCRIPTION OF DRAWINGS

[0020] The present application will be further described below in combination with the drawings and examples.

[0021] Figure 1 is a layout schematic diagram of the present application.

[0022] Figure 2 is a position schematic diagram of the centrifugal pipetting integrated device and the multi-axis robot.

[0023] Figure 3 is a side view schematic diagram of the multi-turntable type pipetting centrifugal device.

[0024] Figure 4 is a top view schematic diagram of the multi-turntable type pipetting centrifugal device.

[0025] Figure 5 is a liquid outlet head arrangement schematic diagram.

[0026] Figure 6 is a local enlarged view of the structure at the tablet bin placement groove.

[0027] Figure 7 is a centrifugal state tablet bin inclination schematic diagram.

[0028] Figure 8 is a reverse inclination schematic diagram of the tablet bin during waste liquid cleaning.

[0029] Figure 9 is a connecting hanging bracket schematic diagram.

[0030] Figure 10 is a second straight line module avoidance connecting hanging bracket schematic diagram.

[0031] Figure 11Figure 1 is a schematic diagram of the pipette device.

[0032] Figure 12 Figure 2 is a cross-sectional view of the pinch clamp.

[0033] Figure 13 Figure 3 is a schematic diagram of the retractable tongue distribution of the pinch clamp.

[0034] Figure 14 Figure 4 is an enlarged schematic diagram of the side slot.

[0035] In the figure: bottom frame 1; bottom plate 101; upper plate 102; liquid outlet head 103; first rotating disc 2; first rotating motor 201; sampling bottle placement hole 202; second rotating disc 3; second rotating motor 301; tablet preparation bin placement slot 302; tablet preparation bin resting plate 303; resting plate rotating shaft 304; lower extension 305; connecting plug block 306; blocking pin 307; U-shaped socket 308; arc-shaped recess 309; plug slot 310; threaded hole 311; adjusting bolt 312; multi-axis moving mechanism 4; first linear module 401; second linear module 402; pipette device 5; pipette 501; flange 502; stop shoulder 503; sealing block 504; first air hole 505; air ring groove 506; first air duct 507; second air hole 508; second air duct 509; base block 510; pinch clamp 511; cylinder 512; retractable tongue 513; tapered block 514; first spring 515; gap portion 516; filling block 517; suspension bracket 6; magnetic block portion 601; fork-shaped bracket 602; suspension rod 603; cross plate 604; waste bucket 7; third rotating disc 8; third rotating motor 801; pipette resting hole 802; side slot 803; spring plunger 9; ball 901; plunger cylinder 902; second spring 903; front stopper 904; rear stopper 905; centrifugal pipetting integrated device 10; first multi-axis robot 11; second multi-axis robot 1101; clamp device 1102; sampling bottle preparation tray 12; tablet preparation bin preparation tray 13; pipette preparation tray 14; dyeing pool 15; slide discharging position 16. DETAILED DESCRIPTION

[0036] As Figures 1-14In one embodiment, a dyeing and slicing integrated workstation includes a feeding area and a discharging area, and a first multi-axis robot 11, a second multi-axis robot 1101 and a dyeing pool 15 are sequentially arranged between the feeding area and the discharging area. The centrifugal pipetting integrated device 10 includes a bottom frame 1, and a first rotating disc 2 and a second rotating disc 3 are coaxially arranged on the bottom frame 1 and are rotatable. The first rotating disc 2 is circumferentially provided with a plurality of sampling bottle placing holes 202 for placing sampling bottles. The second rotating disc 3 is circumferentially provided with a plurality of slicing chamber placing slots 302 for placing slicing chambers. The bottom frame 1 is further provided with a multi-axis moving mechanism 4, and the multi-axis moving mechanism 4 is provided with a pipetting device 5 for transferring liquid in the sampling bottles and the slicing chambers. The first multi-axis robot 11 is used for feeding the centrifugal pipetting integrated device 10 from the feeding area. The second multi-axis robot 1101 is used for clamping the completed glass slides for dyeing.

[0037] The feeding area is provided with a sampling bottle standby tray 12, a slicing chamber standby tray 13 and a pipette standby tray 14. The discharging area is provided with a glass slide discharging position 16. The first multi-axis robot 11 and the second multi-axis robot 1101 are preferably six-axis robots, and a special clamp device 1102 is mounted at the sixth axis end, which is preferably a pneumatic clamp jaw.

[0038] In the preferred embodiment, a rotatable third rotating disc 8 is coaxially arranged with the first rotating disc 2 and the second rotating disc 3. The third rotating disc 8 is arranged above the first rotating disc 2 and the second rotating disc 3. The third rotating disc 8 is circumferentially provided with a plurality of pipette resting holes 802 for temporarily storing pipettes 501.

[0039] After the sampling bottle is pipetted into the slicing chamber, the traditional streamlined slicing station is far away from the pipetting station and the slicing chamber cleaning station. The slicing chamber cleaning station uses a separate overturning cleaning mechanism. The slicing chamber is overturned as a whole to pour out the waste liquid. This method needs to additionally arrange an overturning mechanism, which has a high cost. In addition, a transfer device needs to be additionally arranged to move the slicing chamber after centrifugation to the overturning station. Not only the cost is increased, but also the space occupied by the equipment is increased. The three-rotating-disc slicing station structure. The third rotating disc 8 is not only used for placing clean pipettes initially, but also can temporarily store used pipettes. After centrifugation, the pipettes can be loaded again to suck the waste liquid, and no additional cleaning mechanism is needed.

[0040] The first rotating disc 2 is arranged at the outermost side, the second rotating disc 3 is arranged at the middle, and the third rotating disc 8 is arranged at the innermost side. The number and angle distribution of the pipette resting holes 802 are consistent with those of the sampling bottle placing holes 202 and the slicing chamber placing slots 302. A third rotating motor 801 is mounted at the upper end of the bottom frame 1 to drive the third rotating disc 8 to rotate.

[0041] In the preferred solution, the base frame 1 is provided with a multi-axis moving mechanism 4, which includes a first linear module 401, a sliding plate of the first linear module 401 is provided with a second linear module 402, a sliding plate of the second linear module 402 is connected with the pipette device 5, the first linear module 401 and the second linear module 402 are arranged vertically, and the base frame 1 is further provided with a waste barrel 7 on one side, and the first linear module 401 drives the pipette device 5 to move above the waste barrel 7 to discharge waste liquid.

[0042] The main structure of the linear module is a screw block mechanism driven by a servo motor.

[0043] In the preferred solution, the pipette 501 is provided with a stop shoulder 503 outside the opening end, the stop shoulder 503 is clamped at the pipette resting hole 802, the pipette resting hole 802 is provided with a side slot 803 near the outer edge of the third rotating disc 8, the third rotating disc 8 is provided with a spring plunger 9, the spring plunger 9 includes a retractable ball 901, and the ball 901 abuts against the outer wall of the pipette 501.

[0044] Since the third rotating disc 8 is located at a high position, it is not easy to adopt the mode of moving upward to pull out the pipette 501, and the mode of pulling out laterally can be adopted. In addition, generally, the pipette 501 produced in mass production is a cylindrical tube in the upper region except that the lower end liquid suction part is tapered. If the pull-in and pull-out type is adopted, the residual sample at the lower end of the last batch of sampling tubes will be scraped and left on the pipette resting hole 802 and the spring plunger 9, thereby polluting the next batch of sampling tubes.

[0045] In order to avoid that the pipette 501 is thrown out when the third rotating disc 8 rotates, the spring plunger 9 is arranged at the side slot 803 to abut against the pipette 501. Since the third rotating disc 8 only rotates at a low speed, the centrifugal force is not enough to compress the ball 901, and thus the ball 901 will not be pulled out.

[0046] In the preferred solution, the spring plunger 9 includes a plunger barrel 902, the plunger barrel 902 is provided with a front plug 904 and a rear stop block 905, the front plug 904 is slidably sleeved with the inner wall of the plunger barrel 902, one end of the front plug 904 abuts against the ball 901, and the front plug 904 and the rear stop block 905 are provided with the second spring 903 abutting against each other.

[0047] The rear stop block 905 is threadedly connected with the inner wall of the plunger barrel 902, the compression amount of the second spring 903 can be adjusted, and the unlocking force of the ball 901 can be adjusted.

[0048] In the preferred solution, the third rotating disc 8 is provided with a rotatable slide plate 303 at the slide plate placing groove 302, the pipette device 5 is provided with a connecting hanger 6, the lower end of the connecting hanger 6 is provided with a magnetic block part 601, the magnetic block part 601 adsorbs one end of the slide plate 303 to make the slide plate 303 inclined, and the pipette 501 is inserted into the liquid injection barrel of the slide plate to suck out waste liquid.

[0049] The tablet bin tilts with the inclination of the tablet bin rest plate 303, so that the pipette 501 can suck the waste liquid accumulated in the corner of the tablet bin liquid injection cylinder, and the waste liquid cleaning is more thorough.

[0050] The bottom frame 1 includes a bottom plate 101 and an upper plate 102, and the upper plate 102 is centrally provided with a second rotary motor 301 for driving the second turntable 3 to rotate. The upper plate 102 is provided with a liquid outlet head 103, which is aligned with the tablet bin.

[0051] The bottom frame 1 is provided with coaxially arranged and rotatable first and second turntables 2 and 3. The first turntable 2 is provided with a plurality of sampling bottle placement holes 202 along the circumference, which are used for placing sampling bottles. The second turntable 3 is provided with a plurality of tablet bin placement grooves 302 along the circumference, which are used for placing tablet bins.

[0052] The number of sampling bottle placement holes 202 and tablet bin placement grooves 302 is equal and uniformly distributed along the circumferential direction. Each sampling bottle corresponds to a tablet bin on the radial line.

[0053] The bottom plate 101 is provided with a first rotary motor 201 for driving the first turntable 2 to rotate.

[0054] The liquid outlet head 103 can communicate with external cleaning liquid. After the pipette 501 sucks out the waste liquid in the liquid injection cylinder of the tablet bin, the liquid outlet head 103 injects cleaning liquid into the liquid injection cylinder, and the pipette 501 sucks out the cleaning liquid in the liquid injection cylinder again, so that the waste liquid cleaning is more thorough.

[0055] The pipette device 5 originally exists as a mechanism for transferring liquid from the sampling bottle to the tablet bin. The upper end of the pipette 501 is provided with a pipeline connected to an external air system and a control valve, which can be connected to negative pressure for suction, and also can be connected to positive pressure for liquid discharge.

[0056] The tablet bin placement groove 302 is provided with a slot 310, and the slot 310 is provided with a connecting plug 306. The both sides of the tablet bin rest plate 303 are provided with rest plate rotating shafts 304, which are rotationally connected with the connecting plug 306. One end of the connecting plug 306 is further provided with a stop pin 307. The both sides of the tablet bin rest plate 303 are provided with U-shaped sockets 308, and the stop pin 307 is clamped in the U-shaped socket 308.

[0057] The U-shaped socket 308 is located at the other end of the adsorption end, and the opening faces downward. When the magnetic block part 601 adsorbs the end of the tablet bin rest plate 303, the bottom end of the U-shaped socket 308 abuts against the stop pin 307 to limit the inclination angle of the tablet bin rest plate 303 to a fixed angle.

[0058] In the preferred embodiment, the adapter hanger 6 comprises a fork-shaped bracket 602, the open end of the fork-shaped bracket 602 is connected with the pipette device 5, the lower end of the fork-shaped bracket 602 is provided with a hanger rod 603, the lower end of the hanger rod 603 is connected with a horizontal plate 604, and the magnetic block part 601 is arranged at the lower end of the two sides of the horizontal plate 604.

[0059] The middle part of the tablet bin resting plate 303 is provided with an arc-shaped groove 309 matched with the convex arc shape at the lower end of the tablet bin, after the tablet bin is clamped on the tablet bin resting plate 303, the tablet bin is naturally inclined because the lower extension part 305 is arranged at the rear end of the tablet bin resting plate 303, the lower end of the lower extension part 305 is provided with a threaded hole 311, and the center of gravity is adjusted by adjusting the number and screwing depth of the adjusting bolts 312 to adjust the inclination of the lower tablet bin during centrifugation. During centrifugation, the tablet bin resting plate 303 is always on the lower side and the outer side, and the tablet bin is clamped by the arc-shaped groove 309, so it will not fall out.

[0060] Under the premise of such structural design, the two side edges of the tablet bin resting plate 303 are the adsorption positions of the magnetic block part 601, so the adapter hanger 6 needs to be designed to avoid interference with the fixed part of the second linear module 402 when the adapter hanger 6 moves up and down with the pipette device 5. At the same time, by installing the magnetic block part 601 at the two ends of the horizontal plate 604, it is ensured that the magnetic block part 601 is aligned with the two side edges of the tablet bin resting plate 303.

[0061] In the preferred embodiment, the pipette device 5 comprises a base block 510, the lower end of the base block 510 is provided with a pipe clamp 511, the pipe clamp 511 comprises a cylinder 512, the outer wall diameter of the cylinder 512 is smaller than the inner diameter of the pipette 501, one end of the inner cavity of the cylinder 512 is closed, the end of the cylinder 512 close to the pipe clamp 511 is provided with an opening, the opening end of the cylinder 512 is provided with a plurality of expansion tongues 513 in the circumferential direction, the inner side of the expansion tongue 513 is provided with a wedge surface, the cylinder 512 is provided with a tapered block 514, one end of the tapered block 514 is provided with a first spring 515, the first spring 515 abuts against the closed end of the cylinder 512, the wedge surface of each expansion tongue 513 abuts against the tapered surface of the tapered block 514, the outer wall of the expansion tongue 513 is provided with an annular arc groove, the inner wall of the upper port of the pipette 501 is provided with a flange 502, the annular arc groove is clamped on the flange 502, the cylinder 512 is provided with a filling block 517 between the expansion tongues 513, the base block 510 is provided with a second air channel 509, one end of the second air channel 509 is communicated with the opening end of the cylinder 512.

[0062] In the preferred embodiment, a sealing block 504 is provided at the lower end of the base block 510, and the sealing block 504 blocks the upper end of the pipette 501. A second air vent 508 is provided in the center of the sealing block 504, and the second air vent 508 is connected to the second air channel 509. The sealing block 504 is also provided with a plurality of first air vents 505 arranged along the axial direction. The base block 510 is provided with a ventilation ring groove 506, and each first ventilation hole 505 is connected through the ventilation ring groove 506. A first air channel 507 is also provided in the base block 510, and one end of the first air channel 507 is connected to the ventilation ring groove 506.

[0063] One end of the first air channel 507 and the second air channel 509 is provided with a quick connector for connecting to the control valve of the external air circuit.

[0064] The filler block 517 can reduce the gap between the telescopic tongues 513 in the cylinder 512.

[0065] A gap 516 is defined between the flange 502 and the outer wall of the column 512 , and each first vent hole 505 is aligned with each gap 516 .

[0066] When the pipetting device 5 aspirates liquid, the first air channel 507 is independently connected to negative pressure, and the liquid enters the pipette 501 . Since the pipette 501 is long, the liquid level will not touch the column 512 .

[0067] When the pipetting device 5 is discharging liquid, the first air channel 507 is connected to positive pressure alone.

[0068] When the pipette 501 is removed, the first air channel 507 and the second air channel 509 are simultaneously connected to positive pressure, and the gas enters the open end of the column 512 through the second air vent 508 in the center. Since the filling block 517 reduces the gap between the telescopic tongues 513, the air pressure in the cavity of the column 512 is lower than that at the open end in a short period of time. Under the combined force of the air pressure difference and the impact force of the gas, the cone top block 514 moves downward to compress the spring 515. The cone top block 514 and the telescopic tongue 513 can be magnets. When the cone top block 514 moves downward, it can drive the telescopic tongue 513 to move downward. The tongue 513 retracts, loosening the flange 502. At the same time, since the suction port at the lower end of the pipette 501 is small, the positive pressure has a downward impact force on the pipette 501. At this time, the telescopic tongue 513 is in a loosened state. Under the combined action of gravity and gas impact force, the pipette 501 falls. Under the action of the spring 515, the conical top block 514 returns to its original position. A limiting protrusion is provided on the telescopic tongue 513 to limit the lateral displacement of the telescopic tongue 513, preventing the telescopic tongue 513 from being pushed out, and also preventing the pipette 501 from being squeezed and deformed.

[0069] When loading the pipette 501, the multi-axis moving mechanism 4 drives the base block 510 to move downward so that the sealing block 504 rests against the open end of the pipette 501. At the same time, the second air channel 509 is connected to positive pressure alone, loosening the retractable tongue 513. Then the sealing block 504 moves down into place, the positive pressure is closed, and the retractable tongue 513 is clamped on the flange 502.

[0070] In a preferred embodiment,

[0071] In the loading stage, the first multi-axis robot 11 transfers the sample bottle in the sample bottle preparation tray 12 to the sample bottle placement hole 202, transfers the empty slice preparation chamber in the slice preparation chamber preparation tray 13 to the slice preparation chamber placement groove 302, and transfers the empty pipette 501 in the pipette preparation tray 14 to the pipette resting hole 802.

[0072] In the slice preparation stage, the first rotating disc 2, the second rotating disc 3 and the third rotating disc 8 rotate to align the slice preparation chamber, the sample bottle and the pipette 501 on the radial line, the pipette 501 is loaded from the pipette resting hole 802 on the radial line by the pipette device 5, is transferred above the sample bottle, is lowered and sucks the liquid in the sample bottle, and is transferred to the liquid injection cylinder of the slice preparation chamber on the corresponding radial line, then the pipette 501 is placed back to the pipette resting hole 802, the second rotating motor 301 drives the second rotating disc 3 to rotate at high speed, the cells are deposited on the slide, and the slice preparation is completed.

[0073] In the cleaning stage, the first rotating disc 2, the second rotating disc 3 and the third rotating disc 8 rotate again to align the slice preparation chamber, the sample bottle and the pipette 501 on the radial line according to the original position, the pipette 501 is loaded from the pipette resting hole 802 on the radial line by the pipette device 5, the waste liquid in the slice preparation chamber is sucked and discharged into the waste barrel 7, the liquid outlet head 103 injects the cleaning liquid, then the waste liquid is sucked and discharged into the waste barrel 7 again, then the pipette 501 is discarded into the waste barrel 7, since each pipette 501 corresponds to a slice preparation chamber and a sample bottle, and is not cross used, there is no sample pollution.

[0074] In the staining stage, the second multi-axis robot 1101 clamps the liquid injection cylinder of the slice preparation chamber and removes it to the recycling channel placed aside, then clamps the slide and transfers it to the slide rack on one side of the staining pool 15, after the slide rack is fully inserted, the second multi-axis robot 1101 clamps the slide rack and places it in the staining pool 15 for soaking and staining, and places the slide rack after staining at the slide unloading position 16.

[0075] The above embodiment is only a preferred technical solution of the present application, and should not be regarded as a limitation of the present application. The protection scope of the present application should be the technical solution recited in the claims, including the equivalent replacement solution of the technical features recited in the claims. That is, the equivalent replacement improvement within this range is also within the protection scope of the present application.

Claims

1. An integrated slide preparation and dyeing workstation, characterized by: The invention comprises a loading area and a unloading area, wherein a first multi-axis robot (11), a second multi-axis robot (1101) and a dyeing pool (15) are sequentially arranged between the loading area and the unloading area, and the centrifugal pipetting integrated device (10) comprises a bottom frame (1), wherein a first turntable (2) and a second turntable (3) are coaxially arranged and rotatable, and the first turntable (2) is provided with a plurality of sampling bottle placement holes (202) along the circumference, and the sampling bottle placement holes (202) are used to place sampling bottles, and the second turntable ( 3) A plurality of slide chamber placement slots (302) are provided along the circumference, the slide chamber placement slots (302) are used to place the slide chamber, a multi-axis moving mechanism (4) is further provided on the bottom frame (1), the multi-axis moving mechanism (4) is provided with a liquid transfer device (5), the liquid transfer device (5) is used to transfer liquids in the sampling bottle and the slide chamber, a first multi-axis robot (11) is used to load materials from the loading area to the centrifugal liquid transfer integrated device (10), and a second multi-axis robot (1101) is used to clamp the slide glass after centrifugation for staining; The pipetting device (5) includes a base block (510), a tube holder (511) is provided at the lower end of the base block (510), the tube holder (511) includes a column (512), the outer wall diameter of the column (512) is smaller than the inner diameter of the pipette (501), one end of the inner cavity of the column (512) is closed, and an end of the column (512) close to the tube holder (511) is provided with an opening, a plurality of telescopic tongues (513) are provided along the circumferential direction of the open end of the column (512), a wedge-shaped surface is provided on the inner side of the telescopic tongue (513), a cone top block (514) is provided in the column (512), and the cone top block (514) A first spring (515) is provided at one end, the first spring (515) abuts against the closed end of the column (512), the wedge-shaped surface of each telescopic tongue (513) abuts against the conical surface of the conical top block (514), an annular arc groove is provided on the outer wall of the telescopic tongue (513), a flange (502) is provided on the inner wall of the upper end of the pipette (501), the annular arc groove is clamped on the flange (502), a filling block (517) is provided between the telescopic tongues (513) in the column (512), a second air channel (509) is provided in the base block (510), and one end of the second air channel (509) is connected to the open end of the column (512); A sealing block (504) is provided at the lower end of the base block (510), and the sealing block (504) blocks the upper end of the pipette (501). A second vent hole (508) is provided in the center of the sealing block (504), and the second vent hole (508) is connected to the second air channel (509). The sealing block (504) is also provided with a plurality of first vent holes (505) arranged along the axial direction. The base block (510) is provided with a ventilation ring groove (506), and each first ventilation hole (505) is connected through the ventilation ring groove (506). A first air channel (507) is also provided in the base block (510), and one end of the first air channel (507) is connected to the ventilation ring groove (506).

2. The integrated slide preparation and dyeing workstation according to claim 1, characterized in that: The invention also includes a rotatable third turntable (8) coaxially arranged with the first turntable (2) and the second turntable (3). The third turntable (8) is arranged above the first turntable (2) and the second turntable (3). A plurality of pipette placement holes (802) are circumferentially provided on the third turntable (8). The pipette placement holes (802) are used for temporarily storing pipettes (501).

3. The integrated slide preparation and dyeing workstation according to claim 2, characterized in that: A multi-axis moving mechanism (4) is provided on the bottom frame (1), and the multi-axis moving mechanism (4) includes a first linear module (401). A second linear module (402) is provided on a sliding plate of the first linear module (401), and the sliding plate of the second linear module (402) is connected to a pipetting device (5). The first linear module (401) and the second linear module (402) are arranged vertically. A waste bucket (7) is also provided on one side of the bottom frame (1), and the first linear module (401) drives the pipetting device (5) to move above the waste bucket (7) to discharge waste liquid.

4. The integrated slide preparation and dyeing workstation according to claim 3 is characterized by: A stop shoulder (503) is provided on the outer side of the open end of the pipette (501), and the stop shoulder (503) is stuck in the pipette resting hole (802). A side slot (803) is provided on the outer edge of the pipette resting hole (802) close to the third turntable (8). A spring plunger (9) is provided on the third turntable (8), and the spring plunger (9) includes a retractable ball (901), and the ball (901) abuts against the outer wall of the pipette (501).

5. The integrated slide preparation and dyeing workstation according to claim 4 is characterized by: The spring plunger (9) comprises a plunger barrel (902), wherein a front top plug (904) and a rear stop block (905) are provided in the plunger barrel (902), the front top plug (904) is slidably sleeved with the inner wall of the plunger barrel (902), one end of the front top plug (904) abuts against the ball (901), and a second spring (903) abutting against the front top plug (904) and the rear stop block (905) is provided between them.

6. The integrated slide preparation and dyeing workstation according to claim 3 is characterized by: A rotatable film-making chamber shelf plate (303) is provided at the film-making chamber placement groove (302), a liquid transfer device (5) is provided with a connecting hanger (6), a magnetic block portion (601) is provided at the lower end of the connecting hanger (6), the magnetic block portion (601) adsorbs one end of the film-making chamber shelf plate (303) to tilt the film-making chamber, and a pipette (501) is inserted into the film-making chamber injection cylinder to suck out waste liquid.

7. The integrated slide preparation and dyeing workstation according to claim 6 is characterized by: The connecting hanger (6) includes a fork-shaped frame (602), the open end of the fork-shaped frame (602) is connected to the pipetting device (5), the lower end of the fork-shaped frame (602) is provided with a suspension rod (603), the lower end of the suspension rod (603) is connected to a transverse plate (604), and the magnetic block portion (601) is provided on both sides of the lower end of the transverse plate (604).

8. The slide preparation and dyeing method of the slide preparation and dyeing integrated workstation according to claim 3, characterized in that: In the loading stage, the first multi-axis robot (11) transfers the sampling bottles in the sampling bottle preparation tray (12) to the sampling bottle placement hole (202), transfers the empty film preparation bins in the film preparation bin preparation tray (13) to the film preparation bin placement slot (302), and transfers the empty pipettes (501) in the pipette preparation tray (14) to the pipette placement hole (802); In the preparation stage, the first turntable (2), the second turntable (3) and the third turntable (8) rotate until the preparation chamber, the sampling bottle and the pipette (501) are aligned on a radial line, the pipette device (5) loads the pipette (501) from the pipette holding hole (802) on the radial line, transfers it to the top of the sampling bottle, moves it downward and absorbs the liquid in the sampling bottle, and transfers it to the injection cylinder of the preparation chamber corresponding to the radial line, then puts the pipette (501) back to the pipette holding hole (802), and the second rotary motor (301) drives the second turntable (3) to rotate at high speed to deposit the cells on the slide, completing the preparation; During the cleaning phase, the first turntable (2), the second turntable (3) and the third turntable (8) are rotated again until the film preparation chamber, the sampling bottle and the pipette (501) are aligned on a radial line according to their original positions. The pipette device (5) loads the pipette (501) from the pipette resting hole (802) on the radial line, absorbs the waste liquid in the film preparation chamber and discharges it into the waste bucket (7). The liquid outlet head (103) injects the cleaning liquid, and then absorbs the waste liquid again and discharges it into the waste bucket (7). The pipette (501) is then discarded into the waste bucket (7). When the pipette (501) is removed, the first airway (507) and the second airway (509) are simultaneously connected to positive pressure, and the gas enters the open end of the column (512) through the second vent hole (508) in the center. Since the filling block (517) reduces the gap between the telescopic tongues (513), the air pressure in the inner cavity of the column (512) is lower than that at the open end in a short period of time. Under the combined force of the air pressure difference and the gas impact force, the cone top block (514) moves downward to compress the spring (515). The cone top block (514) and the telescopic tongue (513) can be magnets. When the cone top block (514) moves downward, it can drive the telescopic tongue (513) to move downward. The retractable tongue (513) retracts inward, loosening the flange (502). At the same time, since the liquid suction port at the lower end of the pipette (501) is small, the positive pressure exerts a downward impact force on the pipette (501). At this time, the retractable tongue (513) is in a loosened state. Under the combined action of gravity and gas impact force, the pipette (501) falls down. Under the action of the spring (515), the cone top block (514) is reset. A limiting protrusion is provided on the retractable tongue (513) to limit the lateral displacement of the retractable tongue (513), thereby preventing the retractable tongue (513) from being pushed out and also preventing the pipette (501) from being squeezed and deformed. During the staining stage, the second multi-axis robot (1101) clamps the liquid injection cylinder of the preparation chamber and removes it and places it in the recycling channel on the side, then clamps the slide and transfers it to the slide rack on one side of the staining tank (15). After the slide rack is full, the second multi-axis robot (1101) picks up the slide rack and places it in the staining tank (15) for immersion staining, and places the stained slide rack at the slide unloading position (16).

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