Pen-shaped artificial pollinator and pollination method for pepper breeding

Abstract

The application discloses a pen-shaped artificial pollinator for pepper breeding and a pollination method, which comprises a containing tube, a tube cap movably sleeved with a closed end of the containing tube, an elastic air bag connected between the tube cap and the closed end, a closed end plate hinged with an open end of the containing tube, an elastic pull member connected between the closed end plate and an inner wall of the open end, a push sleeve tube slidably arranged in the open end, a first sliding rod connected with the push sleeve tube, the first sliding rod being slidably arranged in a first sliding hole of the containing tube, a brush head slidably arranged in the containing tube, a second sliding rod of the brush head being slidably arranged in a second sliding hole of the containing tube, one end of a linkage rod of the tube cap being connected with the first sliding rod, a pen ejecting block connected with a middle part of the linkage rod, a protective cover comprising a plurality of arc-shaped petals and isolation bags, one end of each of the arc-shaped petals being connected with an outer wall of the open end through a hinge shaft, a torsional spring being arranged on the hinge shaft, and the isolation bags being detachably sleeved with an outer part of the protective cover. The application solves the problems that the existing pepper pollination process is complicated and easy to be polluted by pollen of other plants.

Description

Technical Field

[0001] This invention relates to the field of chili breeding technology, specifically to a pen-shaped artificial pollinator and pollination method for chili breeding. Background Technology

[0002] In the breeding of three-line chili peppers (restorer line, maintainer line, and sterile line), pollen from different fertile plants is transferred to the flowers of the sterile line to select restorer and maintainer lines. In routine operations, hands must be disinfected before collecting pollen from different plants. During chili pepper breeding, the pollinated flowers are further wrapped in cotton to prevent contamination from pollen from other plants. As you can see, the process is cumbersome and prone to cross-contamination, making breeding even more difficult.

[0003] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0004] To overcome the shortcomings of existing technologies, a pen-shaped artificial pollinator and pollination method for pepper breeding are provided to solve the problems of cumbersome pollination process and easy contamination by pollen from other plants in existing small peppers.

[0005] To achieve the above objectives, a pen-shaped artificial pollinator for chili pepper breeding is provided, comprising:

[0006] A receiving tube has a closed end and an open end. A cap is movably fitted onto the closed end of the receiving tube. An elastic airbag is connected between the cap and the closed end. A closing end plate for sealing the open end is hinged to the open end of the receiving tube. An elastic tie is connected between the closing end plate and the inner wall of the open end. A push sleeve is slidably provided inside the open end. The tube wall of the receiving tube has a first sliding hole and a second sliding hole arranged along the axial direction of the receiving tube. A first sliding rod is connected to the push sleeve. The first sliding rod slides in the first sliding hole and extends to the outside of the receiving tube.

[0007] A brush tip is slidably disposed within the receiving tube. The brush tip is connected to a second sliding rod, which is slidably disposed in a second sliding hole and extends to the outside of the receiving tube. An elastic element is connected between the brush tip and the closed end. A linkage rod is connected to the tube cap. The end of the linkage rod away from the tube cap is connected to the first sliding rod via a spring. A brush-dispensing block for driving the second sliding rod is connected to the middle of the linkage rod. After the tube cap is pressed, the linkage rod pushes the push sleeve via the spring. The push sleeve pushes the closed end plate to open the open end. After the tube cap is continuously pressed, the brush-dispensing block drives the second sliding rod to allow the brush tip to extend to the outside of the open end via the push sleeve.

[0008] The protective cover includes multiple arc-shaped petals and an isolation bag. One end of each arc-shaped petal is connected to the outer wall of the open end via a hinge shaft. The hinge shaft is equipped with a torsion spring for rotating the arc-shaped petals to open them and form the protective cover. The isolation bag has two opposing elastic openings and is detachably fitted onto the outside of the protective cover. After the chili pepper flower is pollinated by the brush, the multiple arc-shaped petals are closed so that one elastic opening slides down and is secured to the flower stalk of the chili pepper flower. The other elastic opening is then detached from the multiple arc-shaped petals and contracts to close, allowing the isolation bag to be fitted onto the outside of the pollinated chili pepper flower to prevent pollen contamination.

[0009] Furthermore, the other end of each of the multiple arc-shaped petals is connected to an elastic rope.

[0010] Furthermore, the second slide bar is connected to a ring sleeve, and the linkage rod is movably inserted through the ring sleeve.

[0011] Furthermore, it also includes a storage box for containing chili pollen, the storage box being detachably installed on the receiving tube, the storage box being connected to the receiving tube through a powder feeding channel to allow the chili pollen to enter the receiving tube, the elastic airbag being connected to the closed end through a pipe, and after the elastic airbag is pressed and before the open end is opened, the airflow inside the elastic airbag blows the chili pollen so that the chili pollen is applied to the brush tip.

[0012] Furthermore, a filter sponge block is installed in the powder feeding channel.

[0013] Furthermore, the elastic element is a return spring.

[0014] Furthermore, the inner wall of the receiving tube is formed with a powder-collecting ring plate, which is disposed below the powder feeding channel, and the inner edge of the powder-collecting ring plate is formed with a flange plate.

[0015] Furthermore, the isolation bag is a light-transmitting nylon bag.

[0016] This invention provides a method for artificial pollination of chili peppers using a pen-shaped artificial pollinator for chili pepper breeding, comprising the following steps:

[0017] a. Press the cap of the receiving tube, and the linkage rod pushes the push sleeve through the spring. The push sleeve pushes the closed end plate to open the opening end of the receiving tube.

[0018] b. Continue pressing the cap, and the brush-ejecting block on the linkage rod drives the second slide rod so that the brush head extends through the push sleeve into the protective cover outside the opening end of the receiving tube.

[0019] c. Dip the brush tip into the pollen of the male parent of the chili pepper;

[0020] d. Release the cap, causing the brush tip to retract into the receiving tube, and the closing end plate closes the opening end;

[0021] e. Repeat steps a to b, so that the brush tip dipped in the pollen is inserted into the protective cover, and the brush tip is used to apply the pollen to the stigma of the chili flower of the chili parent plant for artificial pollination.

[0022] f. After artificial pollination, close multiple arc-shaped petals to allow the elastic opening of the isolation bag to slide down and be secured to the flower stalk of the chili flower;

[0023] g. Disconnect the other elastic opening of the isolation bag from the plurality of arc-shaped petals, and close the other elastic opening so that the isolation bag is placed over the outside of the pollinated pepper flower to avoid pollen contamination.

[0024] The beneficial effects of this invention are as follows: The pen-shaped artificial pollinator for chili pepper breeding uses a linkage rod on the cap to control the opening and closing of the accommodating tube and the intake and output of the brush head via a closed end plate. When the brush head picks up pollen, it is contained within the accommodating tube, preventing contamination from external airflow. When pollinating the parent chili pepper flower, the pollen is expelled through the cap, achieving efficient and rapid artificial pollination with a low pollen contamination rate. Furthermore, after artificial pollination, the chili pepper flower is covered with an isolation bag, avoiding the use of cotton wrapping. This ensures the flower receives light while isolating it from external pollen contamination, thus improving the success rate of artificial pollination. Attached Figure Description

[0025] Other features, objects, and advantages of this application will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0026] Figure 1 This is a schematic diagram of the pen-shaped artificial pollinator for chili pepper breeding according to Embodiment 1 of the present invention.

[0027] Figure 2 This is a schematic diagram of the lower end of the receiving tube according to Embodiment 1 of the present invention.

[0028] Figure 3 This is a bottom view of the lower end of the receiving tube according to Embodiment 1 of the present invention.

[0029] Figure 4 This is a schematic diagram of the open state of the closed end in Embodiment 1 of the present invention.

[0030] Figure 5 This is a schematic diagram of the brush tip extending into the protective cover in Embodiment 1 of the present invention.

[0031] Figures 6 to 10 This is a schematic diagram of the steps for artificial pollination of chili peppers according to Embodiment 2 of the present invention. Detailed Implementation

[0032] The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, only the parts relevant to the invention are shown in the accompanying drawings.

[0033] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0034] Example 1

[0035] Reference Figures 1 to 5 As shown, the present invention provides a pen-shaped artificial pollinator for chili pepper breeding, comprising: a receiving tube 1, a brush tip 2, and a protective cover 3.

[0036] In this embodiment, the pen-shaped artificial pollinator for pepper breeding of the present invention is applicable to the artificial pollination of a single female pepper plant by a small amount of pollen from a male parent.

[0037] The receiving tube 1 is pen-shaped, i.e. cylindrical. The receiving tube 1 has a closed end and an open end. The closed end of the receiving tube is located above the open end of the receiving tube.

[0038] A cap 11 is movably fitted onto the closed end of the receiving tube 1. An elastic airbag 12 is connected between the cap 11 and the closed end. The elastic airbag is a rubber airbag. In this embodiment, the elastic airbag provides a restoring force to the cap.

[0039] See Figures 1 to 3 As shown, a sealing end plate 13 is hinged to the open end of the receiving tube 1. The sealing end plate 13 is used to seal the open end. There are multiple sealing end plates 13. In this embodiment, the sealing end plates are fan-shaped. There are eight sealing end plates.

[0040] An elastic tie is connected between the closed end plate 13 and the inner wall of the open end. The elastic tie is a helical spring. In some embodiments, the closed end plate is hinged to the inner wall of the open end via a hinge shaft. A torsion spring is mounted on the hinge shaft, causing the closed end plate to be positioned radially along the receiving tube.

[0041] A pusher sleeve 14 is slidably installed inside the open end of the closed end plate. The pusher sleeve is located on the inner side of the closed end plate. The outer diameter of the pusher sleeve is adapted to the inner diameter of the receiving tube. The wall of the receiving tube 1 has a first sliding hole and a second sliding hole. The first sliding hole and the second sliding hole are respectively arranged along the axial direction of the receiving tube 1. The first sliding hole is located directly below the second sliding hole. The pusher sleeve 14 is connected to a first sliding rod. The first sliding rod slides in the first sliding hole and extends to the outside of the receiving tube 1.

[0042] The brush head 2 is slidably disposed within the receiving tube 1. The brush head is used to pick up pollen to apply pollen to the chili flowers. The brush head 2 is connected to a second sliding rod. The second sliding rod is slidably disposed within a second sliding hole and extends to the outside of the receiving tube 1.

[0043] An elastic element 21 connects the brush tip 2 to the closed end. In a preferred embodiment, the elastic element is a return spring.

[0044] The cap 11 is connected to a linkage rod 15. The end of the linkage rod 15 furthest from the cap 11 is connected to the first slide rod via a spring. A pen-ejector block 16 is connected to the middle of the linkage rod 15. The pen-ejector block 16 is used to drive the second slide rod.

[0045] In this embodiment, the second slide bar is connected to a ring sleeve. The linkage rod 15 is movably inserted through the ring sleeve. When the brush-spitting block touches the ring sleeve, it pushes the ring sleeve to slide downwards along the linkage rod to eject the brush head outside the opening end of the receiving tube.

[0046] See Figure 4 As shown, after pressing the cap 11, the linkage rod 15 pushes the push sleeve 14 via the spring. The push sleeve 14 pushes the closed end plate 13 to open the open end. At this time, the pen ejector block has not yet contacted the second sliding rod. (See reference...) Figure 5 As shown, after pressing the cap 11, the brush ejector block 16 drives the second slide bar to extend the brush head 2 through the push sleeve 14 to the outside of the opening end, so that the brush head is exposed outside the opening end of the receiving tube.

[0047] The protective cover 3 includes multiple arc-shaped petals 31 and isolation bags 32. The arc-shaped petals have two ends along their length. The width of the arc-shaped petals gradually decreases from the middle of the arc-shaped petals to both ends.

[0048] One end of the arc-shaped petal 31 is connected to the outer wall of the opening end via a hinge shaft. A torsion spring is mounted on the hinge shaft. The torsion spring is used to rotate the arc-shaped petal 31 so that multiple arc-shaped petals 31 are spread out to form a protective cover 3. The protective cover is semi-circular.

[0049] As a preferred embodiment, the other end of the plurality of arc-shaped lobes 31 is connected to an elastic cord to prevent the plurality of arc-shaped lobes from continuing to expand.

[0050] In this embodiment, the isolation bag 32 is a light-transmitting nylon bag or a light-transmitting non-woven bag.

[0051] The isolation bag 32 has two opposing elastic openings. Elastic bands are attached to both ends of the isolation bag to form the elastic openings.

[0052] The isolation bag 32 is detachably fitted onto the outside of the protective cover 3. After the brush tip 2 pollinates the chili flower, the multiple arc-shaped petals 31 are closed so that the opening of one elastic bag slides down and is clamped to the stem of the chili flower. The opening of another elastic bag is detached from the multiple arc-shaped petals 31, and the opening of the other elastic bag is contracted and closed so that the isolation bag 32 is fitted onto the outside of the pollinated chili flower to avoid pollen contamination.

[0053] The elastic bag opening is normally contracted and closed. In some embodiments, the elastic bag opening is covered with a fuzzy texture. When the elastic bag opening is fitted over the flower stem, the fuzzy texture further prevents external pollen from entering the isolation bag. The fuzzy texture of the elastic bag opening improves the isolation bag's ability to prevent pollen contamination.

[0054] See Figure 4 and Figure 5 As shown, the present invention provides a method for artificial pollination of chili peppers using a pen-shaped artificial pollinator for chili pepper breeding, comprising the following steps:

[0055] a-1. Press the cap 11 of the accommodating tube 1. The linkage rod 15 pushes the push sleeve 14 through the spring. The push sleeve 14 pushes the closed end plate 13 to open the open end of the accommodating tube 1.

[0056] b-1. Continue pressing the cap 11. The brush-ejecting block 16 on the linkage rod 15 drives the second slide rod so that the brush head 2 extends through the push sleeve 14 into the protective cover 3 outside the opening end of the receiving tube 1.

[0057] c-1. Dip the brush tip 2 into the pollen of the male parent of the chili pepper.

[0058] d-1. Release the cap 11 to allow the brush tip 2 to retract into the receiving tube 1, and close the opening end of the sealing plate 13.

[0059] e-1. Repeat steps a to b, so that the brush tip 2, which is dipped in pollen, is inserted into the protective cover 3, and the brush tip 2 is used to apply the pollen to the stigma of the chili pepper flower of the parent plant for artificial pollination.

[0060] f-1. After artificial pollination, multiple arc-shaped petals 31 are closed so that the elastic opening of the isolation bag 32 slides down and is clamped to the flower stalk 5 of the chili flower.

[0061] g. Disconnect the other elastic opening of the isolation bag 32 from the multiple arc-shaped petals 31, and close the other elastic opening so that the isolation bag 32 is placed over the outside of the pollinated pepper flower to avoid pollen contamination.

[0062] This invention relates to a pen-shaped artificial pollinator for chili pepper breeding. A linkage rod on the cap controls the opening and closing of the accommodating tube and the ejection of the brush tip via a closed end plate. When the brush tip picks up pollen, it is contained within the accommodating tube, preventing contamination from external airflow. During pollination of the parent chili pepper flower, the pollen is ejected through the cap, achieving efficient and rapid artificial pollination with a low pollen contamination rate. Furthermore, after artificial pollination, the chili pepper flower is covered with an isolation bag, avoiding the use of cotton wrapping. This ensures the flower receives light while isolating it from external pollen contamination, thus improving the success rate of artificial pollination.

[0063] Example 2

[0064] Reference Figures 6 to 10 As shown, the present invention provides a pen-shaped artificial pollinator for chili pepper breeding, comprising a container tube 1, a brush tip 2, and a protective cover 3.

[0065] The difference between this embodiment and Embodiment 1 is that the pen-shaped artificial pollinator for chili pepper breeding also includes a collection box 4 for containing chili pepper pollen. The collection box 4 is detachably installed on the receiving tube 1. The collection box 4 is connected to the receiving tube 1 through a pollen delivery channel to allow chili pepper pollen to enter the receiving tube 1. An elastic airbag is connected to the closed end through an air delivery pipe.

[0066] After the elastic airbag is pressed and before the opening is opened, the airflow inside the elastic airbag blows the chili pollen so that the chili pollen is applied to the brush tip 2.

[0067] In this embodiment, a through hole is provided at the closed end of the receiving tube. An elastic airbag is connected to an air supply line, which passes through the through hole, and the other end of the air supply line extends to the inside of the closed end of the receiving tube. In this embodiment, the elastic airbag not only provides a restoring force for the tube cap, but also uses the airflow stored inside the device to lift the pollen from the chili peppers, so as to evenly coat the brush tip with the pollen.

[0068] As a preferred embodiment, a filter sponge block 19 is installed in the air delivery channel to prevent pollen from being reabsorbed into the elastic airbag.

[0069] In this embodiment, the pen-shaped artificial pollinator for pepper breeding of the present invention is suitable for continuous artificial pollination of multiple female pepper plants by pollen from the same male parent, hence the inclusion of a storage box.

[0070] The inner wall of the receiving tube 1 has a powder-collecting ring plate 17. The powder-collecting ring plate is located below the powder delivery channel. A flange plate 18 is formed on the inner edge of the powder-collecting ring plate. The collection box discharges pollen from the collection box into the powder-collecting ring plate through the inclined powder delivery channel. The collection box has a powder inlet and an exhaust port. After pressing the tube cap, the airflow in the elastic air bladder blows the pollen on the powder-collecting ring plate, and the pollen disperses in the receiving tube and adheres to the brush tip.

[0071] This invention provides a method for artificial pollination of chili peppers using a pen-shaped artificial pollinator for chili pepper breeding, comprising the following steps:

[0072] a-2. Press the cap 11 of the accommodating tube 1. The linkage rod 15 pushes the push sleeve 14 through the spring. The push sleeve 14 pushes the closed end plate 13 to open the open end of the accommodating tube 1.

[0073] Before opening the sac, refer to Figure 6 As shown, after the cap presses the elastic air bladder, the airflow in the elastic air bladder blows the pollen on the pollen ring plate, and the pollen floats in the container tube and adheres to the brush tip.

[0074] See Figure 7 As shown, continue pressing the cap 11 of the accommodating tube 1, and the linkage rod 15 pushes the push sleeve 14 through the spring. The push sleeve 14 pushes the closed end plate 13 to open the open end of the accommodating tube 1.

[0075] b-2, see reference Figure 8 As shown, continue pressing the cap 11, and the brush-ejecting block 16 on the linkage rod 15 drives the second slide rod so that the brush head 2 extends through the push sleeve 14 into the protective cover 3 outside the opening end of the receiving tube 1.

[0076] c-2. Dip the brush tip 2 into the pollen of the male parent of the chili pepper.

[0077] d-2. Release the cap 11 to allow the brush tip 2 to retract into the receiving tube 1, and close the opening end of the sealing end plate 13.

[0078] e-2. Repeat steps a-2 to b-2, so that the brush tip 2 dipped in pollen is inserted into the protective cover 3, and the brush tip 2 is used to apply the pollen to the stigma of the chili pepper flower of the parent plant for artificial pollination.

[0079] f-2, see reference Figure 9 and Figure 10 As shown, after artificial pollination, multiple arc-shaped petals 31 are closed so that the elastic opening of the isolation bag 32 slides down and is clamped to the flower stalk 5 of the chili flower.

[0080] g-2, see further. Figure 10 As shown, the other elastic opening of the isolation bag 32 is detached from the multiple arc-shaped petals 31, and the other elastic opening is contracted and closed so that the isolation bag 32 is placed over the outside of the pollinated pepper flower to avoid pollen contamination.

[0081] The above description is merely a preferred embodiment of this application and an explanation of the technical principles employed. Those skilled in the art should understand that the scope of the invention involved in this application is not limited to technical solutions formed by specific combinations of the above-described technical features, but should also cover other technical solutions formed by arbitrary combinations of the above-described technical features or their equivalents without departing from the inventive concept. For example, technical solutions formed by substituting the above features with (but not limited to) technical features with similar functions disclosed in this application.

Claims

1. A pen-shaped artificial pollinator for chili pepper breeding, characterized in that, include: A receiving tube has a closed end and an open end. A cap is movably fitted onto the closed end of the receiving tube. An elastic airbag is connected between the cap and the closed end. A closing end plate for sealing the open end is hinged to the open end of the receiving tube. An elastic tie is connected between the closing end plate and the inner wall of the open end. A push sleeve is slidably provided inside the open end. The tube wall of the receiving tube has a first sliding hole and a second sliding hole arranged along the axial direction of the receiving tube. A first sliding rod is connected to the push sleeve. The first sliding rod slides in the first sliding hole and extends to the outside of the receiving tube. A brush tip is slidably disposed within the receiving tube. The brush tip is connected to a second sliding rod, which is slidably disposed in a second sliding hole and extends to the outside of the receiving tube. An elastic element is connected between the brush tip and the closed end. A linkage rod is connected to the tube cap. The end of the linkage rod away from the tube cap is connected to the first sliding rod via a spring. A brush-dispensing block for driving the second sliding rod is connected to the middle of the linkage rod. After the tube cap is pressed, the linkage rod pushes the push sleeve via the spring. The push sleeve pushes the closed end plate to open the open end. After the tube cap is continuously pressed, the brush-dispensing block drives the second sliding rod to allow the brush tip to extend to the outside of the open end via the push sleeve. The protective cover includes multiple arc-shaped petals and an isolation bag. One end of each arc-shaped petal is connected to the outer wall of the open end via a hinge shaft. The hinge shaft is equipped with a torsion spring for rotating the arc-shaped petals to open them and form the protective cover. The isolation bag has two opposing elastic openings and is detachably fitted onto the outside of the protective cover. After the chili pepper flower is pollinated by the brush, the multiple arc-shaped petals are closed so that one elastic opening slides down and is secured to the flower stalk of the chili pepper flower. The other elastic opening is then detached from the multiple arc-shaped petals and contracts to close, allowing the isolation bag to be fitted onto the outside of the pollinated chili pepper flower to prevent pollen contamination.

2. The pen-shaped artificial pollinator for chili pepper propagation according to claim 1, characterized in that, The other end of each of the multiple arc-shaped petals is connected to an elastic rope.

3. The pen-shaped artificial pollinator for chili pepper propagation according to claim 1, characterized in that, The second slide bar is connected to a ring sleeve, and the linkage rod is movably inserted through the ring sleeve.

4. The pen-shaped artificial pollinator for chili pepper propagation according to claim 1, characterized in that, It also includes a storage box for containing chili pollen, the storage box being detachably installed on the receiving tube, the storage box being connected to the receiving tube through a powder feeding channel to allow the chili pollen to enter the receiving tube, the elastic airbag being connected to the closed end through a pipe, and after the elastic airbag is pressed and before the open end is opened, the airflow inside the elastic airbag blows the chili pollen so that the chili pollen is applied to the brush tip.

5. The pen-shaped artificial pollinator for chili pepper propagation according to claim 4, characterized in that, A filter sponge block is installed in the powder feeding channel.

6. The pen-shaped artificial pollinator for chili pepper propagation according to claim 4, characterized in that, The elastic element is a return spring.

7. The pen-shaped artificial pollinator for chili pepper propagation according to claim 4, characterized in that, The inner wall of the receiving tube is formed with a powder-collecting ring plate, which is located below the powder feeding channel, and the inner edge of the powder-collecting ring plate is formed with a flange plate.

8. The pen-shaped artificial pollinator for chili pepper propagation according to claim 1, characterized in that, The isolation bag is a light-transmitting nylon bag.

9. A method for artificial pollination of chili peppers using a pen-shaped artificial pollinator for chili pepper propagation as described in any one of claims 1 to 8, characterized in that, Includes the following steps: a. Press the cap of the receiving tube, and the linkage rod pushes the push sleeve through the spring. The push sleeve pushes the closed end plate to open the opening end of the receiving tube. b. Continue pressing the cap, and the brush-ejecting block on the linkage rod drives the second slide rod so that the brush head extends through the push sleeve into the protective cover outside the opening end of the receiving tube. c. Dip the brush tip into the pollen of the male parent of the chili pepper; d. Release the cap, causing the brush tip to retract into the receiving tube, and the closing end plate closes the opening end; e. Repeat steps a to b, so that the brush tip dipped in the pollen is inserted into the protective cover, and the brush tip is used to apply the pollen to the stigma of the chili flower of the chili parent plant for artificial pollination. f. After artificial pollination, close multiple arc-shaped petals to allow the elastic opening of the isolation bag to slide down and be secured to the flower stalk of the chili flower; g. Disconnect the other elastic opening of the isolation bag from the plurality of arc-shaped petals, and close the other elastic opening so that the isolation bag is placed over the outside of the pollinated pepper flower to avoid pollen contamination.

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