Insecticide composition and its application

Abstract

The present invention belongs to the field of pesticide compounding technology and specifically relates to an insecticidal composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), and active ingredient B is any one of flubendiamide, cyprodinil, isoxathiapyram, and brofenoxamide, and the mass ratio of active ingredient A to active ingredient B is 1:36 to 48:1. The composition can effectively control lepidopteran pests such as diamondback moth and cabbage looper, and has the characteristics of expanding the insecticidal spectrum, reducing the application amount, being safe for crops, producing synergistic effects, and addressing resistant pests.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides and insecticides, and particularly relates to an insecticide composition and its application in preventing and controlling herbivorous pests in agriculture, forestry and gardening. Background Art

[0002] Isoflualanam is an isoxazoline insecticide and an allosteric modulator of γ-aminobutyric acid (GABA)-gated chloride channels. It has a unique mechanism of action and exhibits no cross-resistance with other insecticides. Isoflualanam exhibits stomach toxicity, contact toxicity, and some systemic activity, is highly fast-acting, and has ovicidal properties.

[0003] Cyproflanilide is a diamide insecticide with no cross-resistance to existing insecticides. It has a broad insecticide spectrum, good penetration, rapid onset, and high activity. It can effectively control pests that are resistant to existing insecticides, such as the rice stem borer. It is safe for crops and can be widely used on a variety of crops.

[0004] Isocycloseram is an isoxazoline insecticide and acaricide with high insecticidal activity against many types of pests such as Lepidoptera, Hemiptera, Thysanoptera, Coleoptera, Diptera and Acarina. It is also highly ecologically safe for mammals, crops and the environment, and its production and use costs are relatively low.

[0005] Broflanilide is an m-benzamidobenzamide insecticide, an allosteric modulator of GABA-gated chloride channels, which allosterically inhibits GABA-activated chloride channels, causing insects to become overexcited and convulse. It is mainly used to control common pests on crops such as Lepidoptera, Coleoptera, termites, mosquitoes and flies.

[0006] In the process of agricultural production practice, the use of pesticides, while bringing huge benefits to agricultural production, also brings certain problems, among which the generation of pest resistance is one of the main problems. In the specific chemical control process, due to the long-term single use of a certain preparation, pests are easily resistant to different degrees, and the dosage of pesticides used will be increased, polluting the environment and increasing costs. After the inventor's compounding research, the compound of formula (I) was rationally compounded with oxazolidinone, cyprodinil flumipramide, isoxazolidinone amide, and bromofenac, and the activity of the compounded composition was verified. It has a synergistic effect under a certain mass ratio, and there are no related reports on the compounding of the compound shown in formula (I) with oxazolidinone, cyprodinil flumipramide, isoxazolidinone amide, and bromofenac. Summary of the Invention

[0007] The purpose of the present invention is to provide an insecticide composition which has synergistic effect, reduces drug resistance, has low use cost and good prevention effect.

[0008] To achieve the above object, the technical solution adopted by the present invention is: an insecticide composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I) with the following structural formula:

[0009] Active ingredient B is any one of flubendiamide, cyflumilast, isoxathiapyr, and bromofenac;

[0010] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 48:1;

[0011] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 48:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to oxazolidinone is 1:30 to 26:1, such as 1:30, 1:25, 1:13, 1:6, 2:5, 6:1, 13:1, 26:1 or any value therebetween;

[0013] The mass ratio of the compound of formula (I) to oxazolidinone is 1:25 to 26:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to oxazolidinone is 1:25, 1:13, 1:6, 2:5, 6:1, 13:1, and 26:1;

[0015] The mass ratio of the compound of formula (I) to ciprofloxacin is 1:28 to 28:1, such as 1:28, 1:18, 1:8, 4:1, 8:1, 16:1, 28:1 or any value therebetween;

[0016] The mass ratio of the compound of formula (I) to cyflumilast is 1:18 to 28:1;

[0017] The mass ratio of the compound of formula (I) to cyflumilast is 1:18, 1:8, 4:1, 8:1, 16:1, and 28:1;

[0018] The mass ratio of the compound of formula (I) to isoxathiapiprolin is 1:24 to 24:1, such as 1:24, 1:12, 1:6, 5:2, 6:1, 12:1, 24:1 or any value between the above values;

[0019] The mass ratio of the compound of formula (I) to brofenoxamide is 1:36 to 48:1, such as 1:36, 1:24, 1:12, 1:4, 4:1, 12:1, 24:1, 48:1 or any value therebetween;

[0020] The mass ratio of the compound of formula (I) to brofenoxamide is 1:24 to 48:1;

[0021] The mass ratio of the compound of formula (I) to brofenoxamide is 1:24, 1:12, 1:4, 4:1, 12:1, 24:1, and 48:1;

[0022] Furthermore, based on the total weight of the insecticide composition being 100 wt%, the sum of the content of the active ingredient A and the active ingredient B in the insecticide composition is 0.5 to 90 wt%;

[0023] Furthermore, based on the total weight of the insecticide composition being 100 wt%, the sum of the content of the active ingredient A and the active ingredient B in the insecticide composition is 1 to 80 wt%;

[0024] The insecticide composition of the present invention can be prepared by a common processing method known to those skilled in the art, that is, after mixing the active ingredient with a liquid solvent or a solid carrier, one or more surfactants such as a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a penetrant and a carrier are added;

[0025] Furthermore, the wetting agent is selected from a mixture of one or more of sodium lauryl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, sapodilla powder, silkworm excrement or soapberry powder;

[0026] Furthermore, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ether phosphates, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers;

[0027] Furthermore, the emulsifier is selected from Nongru 500# (calcium alkylbenzene sulfonate), OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether disulfonic acid magnesium salt, triethanolamine salt, Nongru 400# (benzyl dimethylphenol polyoxyethyl ether), Nongru 700# (alkylphenol formaldehyde resin polyoxyethyl ether), Ningru 36# (phenylethylphenol formaldehyde resin polyoxyethylene ether), A mixture of one or more of the following: Nongru 1600# (phenylethylphenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), Nongru 33# (alkylaryl polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether);

[0028] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

[0029] Furthermore, the disintegrant is selected from a mixture of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

[0030] Furthermore, the antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea;

[0031] Furthermore, the defoaming agent is selected from silicone oil, silicone compounds, C 10 ~C 20 Saturated fatty acid compounds or C8~C 10 A mixture of one or more fatty alcohol compounds;

[0032] Furthermore, the solvent is selected from a mixture of one or more of N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;

[0033] Furthermore, the stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0034] Furthermore, the penetrant is selected from a mixture of one or more of penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone or silicone;

[0035] Furthermore, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;

[0036] Furthermore, the filler is selected from a mixture of one or more of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

[0037] The above substances are all commercially available.

[0038] The insecticide composition of the present invention can be processed into any agriculturally acceptable dosage form;

[0039] Furthermore, the insecticide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is selected from a solid formulation and / or a liquid formulation;

[0040] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0041] Furthermore, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0042] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0043] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; and the liquid preparation is selected from emulsifiable concentrates and suspension concentrates.

[0044] The present invention also provides the use of the above-mentioned insecticide composition in controlling agricultural, forestry or gardening herbivorous pests;

[0045] Furthermore, the herbivorous pests are pests with chewing mouthparts.

[0046] The chewing mouthparts pests include: Adoxophyes spp., Adoxophyes orana, Agrotis spp. (cutworms), Agrotis ipsilon (black cutworm), Alabama argillacea (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twigborer), Anomis sabulifera (jute looper), Anticarsia gemma ta lis, Archips argyrospila (fruittree leafroller), Archips rosana (rose leafroller), and A. roller), Argyrotaenia spp. (tortricidmoths), Argyrotaenia citrana (orange tortrix), Autographagamma, Bonagota cranaodes, Borbo cinnara (rice leaf folder), Bucculatrix thurberiella (cotton leaf perforator), Caloptilia spp. (leaf miners), Capua reticulana, Carposina niponensis (peachfruit moth), Chilo spp., Chlumetia transversa (mangoshoot borer), Choristoneura rosaceana (obliquebanded leaf roller),

[0047] leafroller)), Chrysodeixis spp., Cnaphalocerus medinalis (grass

[0048] leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus (carpenter moth), Crambus spp. (sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydian ignicana (pea moth), Cydia pomonella (codling moth), Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester cornborer), Earias spp. (cotton bollworm), Earias insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopophaaurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Epiphysias postruttana (light brown applemoth), Ephestia spp. (flour moths), Ephestia cautella (almond moth), tobacco powdery moth

[0049] Ephestia elutella (tobbaco moth), Ephestiakuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella (grape berry moth), Euxoa auxiliaris (army cutworm), Feltia spp. (cutworms), Gortyna spp. (stemblerers), Grapholita molesta (Oriental fruit moth),

[0050] (Oriental Fruit Moth), Hedylepta indicate (Bean Leaf Webber), Helicoverpa sp. (Spodoptera exigua), Helicoverpa armigera, Helicoverpa zea, Heliothis spp., Heliothis virescens, Hellula undalis (Cabbage Webworm), Indarbela spp. (Root Borers), Keiferia lycopersicella, Leucinodes orbonalis, Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (Grape Fruit Moth), fruitmoth), Loxagrotis spp., Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.) (tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis (bean pod borer), Metisa plana (bagworm), Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis, Nymphula depunctalis (three-spotted water borer), Operophtherabrumata (winter looper), Ostrinia nubilalis (European corn borer), Oxydia vesulia, Pandemis cerasana (common currant tortrix), Pandemis heparana (brown apple tortrix), Papilio demodocus (African swallowtail), Pectinophora gossypiella (pink bollworm), (Bollworm), Peridroma species (pp.), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp., Pieris rapae, Plathypena scabra, Plodia interpunctella, Plutellaxylostella (diamondback moth), Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays olive moth oleae) (olive moth), Pseudaletia spp.) (spodoptera), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (, Rachiplusia nu), Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp., Spodoptera exigua, Spodoptera fugiperda, Spodoptera oridania (southern armyworm), Synanthedon spp., Thecla basilides, Thermisia gemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (cabbage worm), and Tuta absoluta;

[0051] Furthermore, the chewing mouthpart pests include diamondback moth, beet armyworm, Spodoptera litura, fall armyworm, striped stem borer, cotton bollworm, and cabbage worm;

[0052] The present invention also provides the above-mentioned insecticide composition to be applied in an effective dose to the pests to be controlled or the medium where they grow.

[0053] The beneficial effects of the present invention are:

[0054] The insecticide composition of the present invention is environmentally friendly and easily degraded in the environment; it has obvious synergistic and long-lasting effects, has a high preventive effect on chewing mouthparts pests of crops, can reduce the development of chewing mouthparts pests' resistance to pesticides, reduces the amount of pesticide used, and reduces the amount of pesticide residues on crops. DETAILED DESCRIPTION

[0055] The present invention is further described below with reference to the examples. The percentages in the examples are all by weight, but the present invention is not limited thereto.

[0056] The insecticide composition of the present invention can be provided in the form of a formulation. It can be formulated as a suspension concentrate, water-dispersible granules, wettable powder, or dispersible oil suspension concentrate, as needed. The content of the active ingredient in the composition of the present invention depends on the application rate when used alone, as well as the mixing ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies depending on the formulation type of the composition.

[0057] Preparation Example

[0058] Preparation Example 1:

[0059] 21% formula (I) compound·oxazolidinone suspension concentrate (6:15)

[0060] Preparation formula: 6% of compound of formula (Ⅰ), 15% of oxazolidinone,

[0061] Polyether 3.5%, alkylphenol polyoxyethylene ether phosphate 3%, dioctyl sodium sulfosuccinate 3%, magnesium aluminum silicate 1%, xanthan gum 0.25%, ethylene glycol 5%, benzisothiazolinone 0.02%, silicone defoamer 0.4%, deionized water to make up the balance;

[0062] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0063] Preparation Example 2:

[0064] 45% formula (I) compound·oxazolidinone wettable powder (10:35)

[0065] Preparation formula: 10% of compound of formula (I), 35% of oxazolidinone,

[0066] Sodium lignin sulfonate 5%, naphthalene sulfonate formaldehyde condensate 3.5%, polycarboxylic acid sodium salt 1.5%, BX powder 2.5%, white carbon black 10%, kaolin makes up the balance;

[0067] Preparation method: The active ingredients, other functional additives and fillers are mixed according to the formula ratio, stirred evenly in a stirring kettle, and crushed and mixed evenly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0068] Preparation Example 3:

[0069] 24% formula (I) compound·Cycloflumizone suspension concentrate (20:4)

[0070] Preparation formula: 20% of compound of formula (Ⅰ), 4% of cyprodinil.

[0071] Isotridecyl alcohol polyoxyethylene ether 2.5%, polyoxyethylene sorbitan monooleate 1.5%, tristyrylphenol ethoxylate phosphate 2.5%, sodium polycarboxylate 1%, magnesium aluminum silicate 1%, carboxyethyl cellulose 0.05%, sodium sorbate 0.5%, ethylene glycol 5%, silicone oil 0.5%, deionized water to make up the balance;

[0072] Preparation method: Same as Preparation Example 1.

[0073] Preparation Example 4:

[0074] 40% formula (I) compound·Cyclosulfuron water dispersible granules (32:8)

[0075] Preparation formula: 32% of compound of formula (Ⅰ), 8% of cyprodinil.

[0076] Naphthalenesulfonate formaldehyde condensate 10.5%, polycarboxylate sodium salt 6%, sodium lauryl sulfate 3%, ammonium sulfate 15%, starch makes up the balance;

[0077] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and screening.

[0078] Preparation Example 5:

[0079] 21% formula (I) compound·isoxazole amide suspension (18:3)

[0080] Preparation formula: 18% of compound of formula (Ⅰ), 3% of isoxathiapiprolin,

[0081] Ethylene glycol oxyethylene polyoxypropylene ether 1%, polyoxyethylene sorbitan monooleate 2.5%, tristyrylphenol ethoxylate phosphate 2.5%, polycarboxylic acid sodium salt 1%, magnesium aluminum silicate 1%, carboxyethyl cellulose 0.05%, sodium sorbate 0.5%, ethylene glycol 5%, silicone oil 0.5%, deionized water to make up the balance;

[0082] Preparation method: Same as Preparation Example 1.

[0083] Preparation Example 6:

[0084] 42% formula (I) compound·isoxazoline wettable powder (36:6)

[0085] Preparation formula: 36% of compound (I), 6% of isoxathiapiprolin,

[0086] Sodium lignin sulfonate 5%, naphthalene sulfonate formaldehyde condensate 3.2%, polycarboxylic acid sodium salt 2%, BX powder 2.5%, white carbon black 10%, starch 5%, kaolin makes up the balance;

[0087] Preparation method: Same as Preparation 2.

[0088] Preparation Example 7:

[0089] 15% formula (I) compound·isoxazole amide dispersible oil suspension (12:3)

[0090] Preparation formula: 12% of compound of formula (Ⅰ), 3% of isoxathiapiprolin,

[0091] Sulfonic acid succinate 2%, alkyl aryl polyoxyethylene polyoxypropylene ether 12%, fatty alcohol polyoxyethylene ether 3%, calcium dodecylbenzene sulfonate 2%, silicon dioxide 1%, organic bentonite 1%, 200# solvent oil 15%, methyl oleate makes up the balance;

[0092] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, and solvent oil is added and mixed evenly. After high-speed shearing, wet sand grinding, and finally homogenization filtration, the dispersible oil suspension product is obtained.

[0093] Preparation Example 8:

[0094] 10% formula (I) compound·bromofenac suspension (8:2)

[0095] Preparation formula: 8% of compound of formula (Ⅰ), 2% of bromofenac,

[0096] Alkylphenol polyoxyethylene ether phosphate 4%, ethylene glycol oxyethylene polyoxypropylene ether 4%, polycarboxylic acid sodium salt 1.6%, polyoxyethylene sorbitan monooleate 1.5%, magnesium aluminum silicate 1%, xanthan gum 0.3%, sodium sorbate 0.5%, ethylene glycol 5%, silicone oil 0.5%, deionized water to make up the balance;

[0097] Preparation method: Same as Preparation Example 1.

[0098] Preparation Example 9:

[0099] 20% formula (I) compound·bromofenac dispersible oil suspension (15:5)

[0100] Preparation formula: 15% of compound of formula (Ⅰ), 5% of bromofenac,

[0101] Sulfonic acid succinate 2%, alkyl aryl polyoxyethylene polyoxypropylene ether 12%, fatty alcohol polyoxyethylene ether 3%, calcium dodecylbenzene sulfonate 2%, organosilicon defoamer 0.2%, organobentonite 1%, silicon dioxide 1%, 200# solvent oil 15%, methyl oleate makes up the balance;

[0102] Preparation method: Same as Preparation Example 7.

[0103] Indoor biological activity assay:

[0104] The specific test methods are as follows:

[0105] Lepidoptera larvae: a combination of leaf and insect dipping;

[0106] Test insect age: Use sensitive populations raised indoors for multiple generations, and select healthy and consistent second-instar Lepidoptera larvae (no less than 60 per treatment).

[0107] Use tweezers to place fresh cabbage slices in the drug solution and immerse them for 10 seconds, then take them out. After the drug solution has dried naturally, place the cabbage slices in a culture dish lined with moisturizing filter paper. Place the test insects in the drug solution for 5 seconds, absorb the excess drug solution with filter paper, and place the test insects in a culture dish with leaves soaked in the corresponding concentration.

[0108] Each treatment was repeated 4 times, with a blank treatment as a control. The treated insects were reared in an artificial intelligence culture chamber at 26±1°C, a light intensity of L:D=16h:8h, and a relative humidity of 60%.

[0109] After treatment with the pesticide, the mortality of the test insects was investigated 48 hours later. The criterion for judging the death of the test insects was obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. The number of dead insects was recorded.

[0110] Data statistics and analysis:

[0111] According to the survey data, the adjusted mortality rate of each treatment was calculated using the following formula:

[0112]

[0113] Where:

[0114] P——mortality rate, in percentage (%);

[0115] K——indicates the number of dead insects, the unit is head;

[0116] N——represents the total number of insects processed, in heads.

[0117]

[0118] Where:

[0119] P1——adjusted mortality rate, in percentage (%);

[0120] P t ——Treatment mortality rate, expressed in percentage (%);

[0121] P0 - blank control mortality rate, in percentage (%).

[0122] Data statistics and analysis: If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the experiment needs to be repeated.

[0123] The data were processed by probability value analysis. The DPS statistical analysis system was used to analyze the toxicity regression line and LC 50 The activity of the test agents on the biological materials was evaluated by using the values, 95% confidence limits and correlation coefficient r.

[0124] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0125]

[0126] Where:

[0127] ATI - measured toxicity index of mixture;

[0128] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0129] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0130] TTI=TI A *P A +TI B *P B

[0131] Where:

[0132] TTI – Theoretical Toxicity Index of Mixtures;

[0133] TI A ——Agent toxicity index;

[0134] P A ——The percentage of agent A in the mixture, in percentage (%);

[0135] TI B ——Toxicity index of agent B;

[0136] P B ——The percentage of agent B in the mixture, in percentage (%).

[0137]

[0138] Where:

[0139] CTC – Co-toxicity coefficient;

[0140] ATI - measured toxicity index of mixture;

[0141] TTI - Theoretical Toxicity Index of Mixture.

[0142] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0143] The test results are as follows:

[0144] Indoor activity assay of Pieris rapae

[0145] As shown in Table 1 and Table 2, the compound of formula (I) has a good toxic effect on the rapae worm. The LC values ​​of tetrazobactam and cyantraniliprole on rapae worm are 50 They are 2.3827 mg / L and 1.1871 mg / L respectively.

[0146] When the weight ratio of the compound of formula (I) and oxazolidinone to Pieris rapae is 1:30 to 26:1, the co-toxicity coefficient is greater than 80, indicating that the two exhibit additive or synergistic effects when mixed within the range of 1:30 to 26:1, and no antagonistic effect occurs; when the compound of formula (I) and oxazolidinone are mixed with Pieris rapae at a weight ratio of 1:25 to 26:1, the co-toxicity coefficient is greater than 120, indicating that the two exhibit synergistic effects when mixed within this weight ratio range.

[0147] When the weight ratio of the compound of formula (I) and cyprodinil flumipramide to Pieris rapae is 1:28 to 28:1, the co-toxicity coefficient is greater than 80, indicating that the two exhibit additive or synergistic effects when mixed within the range of 1:28 to 28:1. When the weight ratio of the compound of formula (I) and cyprodinil flumipramide to Pieris rapae is 1:18 to 28:1, the co-toxicity coefficient is greater than 120, indicating that the two exhibit synergistic effects when mixed within this weight ratio range.

[0148] Table 1 Toxicity test results of compound of formula (I) combined with oxazolidinone to Pieris rapae

[0149]

[0150] Table 2 Toxicity test results of compound of formula (I) combined with cyfluthrin against Pieris rapae

[0151]

[0152] Indoor activity determination of Plutella xylostella

[0153] As shown in Table 3 and Table 4, the compound of formula (I) has a high toxicity effect on Plutella xylostella. The LC values ​​of isoxadiazole and bromofenac on Plutella xylostella are 50 They are 0.4839 mg / L and 0.2306 mg / L respectively.

[0154] When the weight ratio of the compound of formula (I) and isoxathiapiprole to the diamondback moth is 1:24 to 24:1, the co-toxicity coefficient is greater than 120, indicating that the mixture of the two exhibits a synergistic effect within this weight ratio range.

[0155] When the weight ratio of the compound of formula (I) and brofenac to the diamondback moth is 1:36 to 48:1, the co-toxicity coefficient is greater than 80, indicating that the two show additive or synergistic effects when mixed within the range of 1:36 to 48:1, and no antagonistic effect occurs; when the compound of formula (I) and brofenac are mixed with the diamondback moth at a weight ratio of 1:24 to 48:1, the co-toxicity coefficient is greater than 120, indicating that the two show synergistic effects when mixed within this weight ratio range.

[0156] Table 3 Toxicity test results of compound of formula (I) combined with isoxathiapiprole against Plutella xylostella

[0157]

[0158] Table 4 Toxicity test results of compound of formula (I) and tetrachlorantraniliprole against Plutella xylostella

[0159]

[0160] Field trials on controlling cabbage loopers

[0161] Experimental crops: Cabbage (cabbage), which was in the rosette stage during the experiment;

[0162] Test target: Pieris rapae;

[0163] Test reference: Based on GB / T 17980.13-2000 "Guidelines for field efficacy tests (I): Insecticides for the control of lepidopteran larvae of cruciferous vegetables";

[0164] Experimental location: Cabbage experimental field in the cruciferous vegetable planting base of Biejiatun Village, Anqiu City, Weifang City, Shandong Province;

[0165] Application equipment: 3WBS-16 electric backpack sprayer;

[0166] Trial date: July 10, 2023;

[0167] Experimental environment: All experimental plots were uniformly cultivated, with the same fertilizer and water management level, good water and fertilizer conditions, and clay loam soil;

[0168] Test agent:

[0169] Table 5 Field test design and dosage

[0170]

[0171] Test method: Apply the pesticide once during the peak period of cabbage looper occurrence (mostly 2nd to 4th instar).

[0172] Plot area and repetition: random block arrangement, plot area 20m 2 , each treatment was repeated 4 times.

[0173] Survey method: Randomly sample five points in each plot, hang a sign on four cabbages at each point, and survey the number of cabbage worms on all cabbages. Before applying pesticides, survey the base number of the insect population.

[0174] The number of remaining insects was investigated 3 days and 10 days after application.

[0175] Calculation formula and data analysis:

[0176]

[0177] Test results:

[0178] As can be seen from Table 6, 3 days after application, 21% formula (I) compound·oxaflumizone suspension concentrate (6:15) and 24% formula (I) compound·cyprosulfuron suspension concentrate (20:4) showed good fast-acting properties against cabbage loopers, with control rates of 84.30% and 96.02%, respectively, which were significantly higher than those of the control single agent.

[0179] Table 6 Field control effects of different pesticides against cabbage worms (3 days after application)

[0180]

[0181] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0182] As can be seen from Table 7, 10 days after application, the control efficacy of 21% formula (I) compound·oxaflumizone suspension concentrate (6:15) and 24% formula (I) compound·cyprosulfuron suspension concentrate (20:4) against cabbage loopers were 90.96% and 90.42%, respectively, which were significantly higher than the control single agent, showing good long-lasting efficacy.

[0183] Table 7 Field control effects of different pesticides on cabbage worms (10 days after application)

[0184]

[0185] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0186] Safety: During the test period and later observations, cabbage grew normally and no obvious phytotoxicity was observed.

[0187] Field trials on controlling diamondback moth

[0188] Test crops: cauliflower;

[0189] Test target: Diamondback moth;

[0190] Test reference: Based on GB / T 17980.13-2000 "Guidelines for field efficacy tests (I): Insecticides for the control of lepidopteran larvae of cruciferous vegetables";

[0191] Experimental location: Cauliflower experimental field at the cruciferous vegetable planting base in Zhangjiashagou Village, Anqiu City, Weifang City, Shandong Province;

[0192] Application equipment: 3WBS-16 electric backpack sprayer;

[0193] Trial time: August 15, 2023;

[0194] Experimental environment: All experimental plots were uniformly cultivated, with the same fertilizer and water management level, good water and fertilizer conditions, and clay loam soil;

[0195] Test agent:

[0196] Table 8 Field test design and dosage

[0197]

[0198] Test method: Apply the pesticide once during the peak period of diamondback moth infestation.

[0199] Plot area and repetition: random block arrangement, plot area 20m 2 , each treatment was repeated 4 times.

[0200] Survey method: Randomly sample five points in each plot, hang two cauliflower plants at each point, and investigate the number of diamondback moths on the whole cauliflower plant. The insect population base was investigated before spraying.

[0201] The number of remaining insects was investigated 3 days and 7 days after application.

[0202] Calculation formula and data analysis:

[0203]

[0204] Test results:

[0205] As can be seen from Table 9, 3 days after application, 21% formula (I) compound·isoxazole suspension concentrate (18:3) and 10% formula (I) compound·bromofenac suspension concentrate (8:2) showed good quick-acting properties against Plutella xylostella, with control efficacies of 84.55% and 84.96%, respectively, which were significantly higher than those of the control single agent.

[0206] Table 9 Field control effects of different pesticides on Plutella xylostella (3 days after application)

[0207]

[0208] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0209] As can be seen from Table 10, 7 days after application, 21% formula (I) compound·isoxazole suspension concentrate (18:3) and 10% formula (I) compound·bromofenac suspension concentrate (8:2) showed good persistent effect against Plutella xylostella, with control efficacy of 92.50% and 93.22%, respectively, which were significantly higher than the control single agent.

[0210] Table 10 Field control effects of different pesticides on Plutella xylostella (7 days after application)

[0211]

[0212] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0213] Safety: During the trial period and subsequent observations, cauliflower grew normally and no obvious signs of drug damage were observed.

[0214] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is defined by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition, characterized in that It contains active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I) with the following structural formula: (I); active ingredient B is oxazolidinone, and the mass ratio of the compound of formula (I) to oxazolidinone is 1:25 to 26:

1.

2. The insecticidal composition according to claim 1, characterized in that The mass ratio of the compound of formula (I) to oxazolidinone is 1:25, 1:13, 1:6, 2:5, 6:1, 13:1, and 26:

1.

3. The insecticidal composition according to claim 1, characterized in that Based on the total weight of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 0.5 to 90 wt%.

4. The insecticidal composition according to claim 3, characterized in that Based on the total weight of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 1 to 80 wt%.

5. The insecticidal composition according to claim 1, characterized in that The insecticide composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a penetrant and a carrier.

6. The insecticidal composition according to claim 5, characterized in that The insecticide composition is prepared into an agriculturally acceptable formulation, which is selected from a solid formulation and / or a liquid formulation.

7. The insecticidal composition according to claim 6, characterized in that The solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

8. Use of the insecticidal composition according to any one of claims 1 to 7 in controlling herbivorous pests in agriculture, forestry or gardening.

9. The use according to claim 8, characterized in that The herbivorous pests are chewing pests; the chewing pests include diamondback moth, beet armyworm, Spodoptera litura, fall armyworm, striped stem borer, cotton bollworm or cabbage worm.

10. The use according to claim 8, characterized in that The insecticide composition is applied to the pests to be controlled or the medium where they grow in an effective dose.

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