Snow lotus extract, its preparation method and application

Abstract

The present invention relates to the technical field of skin care products, and particularly to a snow lotus extract, a preparation method thereof and an application. The preparation method of the snow lotus extract includes: the alcohol extract of snow lotus is redissolved and purified by polyhydric alcohol and an adsorbent. In the preparation method of the snow lotus extract of the present invention, polyhydric alcohol with low polarity is used, and the alcohol extract of snow lotus is redissolved and purified in combination with an adsorbent. The prepared snow lotus extract has no peculiar smell, light color, no problem of microbial contamination, and at the same time, its biological activity is significantly improved, the product quality is significantly improved, the application problem is solved, and its application in the cosmetic field is greatly enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a saussurea involucrata extract, a preparation method thereof and an application thereof. Background Art

[0002] Saussurea involucrata is a perennial herbaceous plant of the genus Saussurea in the family Asteraceae and belongs to precious plant raw materials. The effective substance components in saussurea involucrata include polymethoxy flavones such as homoplantaginin, jaceosidin, acacetin, etc., and flavonol glycoside compounds with quercetin as the aglycone such as quercitrin, isoquercitrin, rutin, etc., especially the content of rutin is the highest. It also contains a rich variety of phenolic acid compounds such as chlorogenic acid, p-coumaric acid and dimer compounds with chlorogenic acid as the main body such as 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, etc. In addition, there are lignan compounds such as arctiin, which are the material basis for the saussurea involucrata extract to exert biological activities such as whitening, antioxidant, anti-inflammatory, anti-aging, etc.

[0003] At present, the extract prepared from saussurea involucrata by the extraction process often has problems such as unpleasant odor, deep color, few active ingredients, and unremarkable efficacy; moreover, during the separation and enrichment of the efficacy substances, the loss of the efficacy substances is relatively large, resulting in a low content of the efficacy substances in the product, further affecting the applicability and efficacy of the saussurea involucrata extract. Summary of the Invention

[0004] An object of the present invention is to provide a preparation method of a saussurea involucrata extract that can effectively increase the content of the efficacy components in the saussurea involucrata extract and fade the unpleasant odor and color of the saussurea involucrata extract, including: the alcohol extract of saussurea involucrata is redissolved and purified by polyol and an adsorbent.

[0005] In a specific embodiment of the present invention, the redissolving and purifying treatment includes: after the alcohol extract of saussurea involucrata is redissolved by polyol, it is mixed evenly with the adsorbent and filtered.

[0006] In a specific embodiment of the present invention, the redissolving and purifying treatment includes: mixing the alcohol extract of saussurea involucrata, polyol and an adsorbent evenly and filtering.

[0007] In a specific embodiment of the present invention, the adsorbent includes a polar adsorbent.

[0008] In a specific embodiment of the present invention, the adsorbent includes at least one of silica gel, macroporous adsorption resin and neutral alumina.

[0009] In a specific embodiment of the present invention, the adsorbent includes silica gel.

[0010] In a specific embodiment of the present invention, the dosage of the adsorbent is 0.05% - 5% of the total mass of the polyol and the ethanol extract of Saussurea involucrata.

[0011] In a specific embodiment of the present invention, the dosage of the adsorbent is 0.5% - 5% of the total mass of the polyol and the ethanol extract of Saussurea involucrata.

[0012] In a specific embodiment of the present invention, the dosage of the adsorbent is 1% - 3% of the total mass of the polyol and the ethanol extract of Saussurea involucrata.

[0013] In a specific embodiment of the present invention, the temperature of the purification treatment is 20 - 85°C.

[0014] In a specific embodiment of the present invention, the temperature of the purification treatment is 20 - 50°C.

[0015] In a specific embodiment of the present invention, the temperature of the purification treatment is 20 - 30°C.

[0016] In a specific embodiment of the present invention, the time of the purification treatment is 5 - 90 min.

[0017] In a specific embodiment of the present invention, the time of the purification treatment is 5 - 30 min.

[0018] In a specific embodiment of the present invention, the time of the purification treatment is 10 - 20 min.

[0019] In a specific embodiment of the present invention, the polyol includes at least one of 1,3 - butanediol and dipropylene glycol.

[0020] In a specific embodiment of the present invention, the mass fraction of the polyol is ≥50%.

[0021] In a specific embodiment of the present invention, the mass fraction of the polyol is 50% - 65%.

[0022] In a specific embodiment of the present invention, the mass percentage of the polyol is 100%.

[0023] In a specific embodiment of the present invention, the temperature of the reconstitution is 20 - 85°C.

[0024] In a specific embodiment of the present invention, the temperature of the reconstitution is 40 - 80°C.

[0025] In a specific embodiment of the present invention, the method for preparing the ethanol extract of Saussurea involucrata includes:

[0026] Saussurea involucrata is extracted with ethanol, and the mass - to - volume ratio of Saussurea involucrata to ethanol is 1:10 - 1:40;

[0027] Extract at 45 - 85°C for 1 - 4 h and filter.

[0028] In a specific embodiment of the present invention, the mass - to - volume ratio of saussurea involucrata to ethanol is 1:15 - 1:30.

[0029] In a specific embodiment of the present invention, the mass - to - volume ratio of saussurea involucrata to ethanol is 1:18 - 1:25.

[0030] In a specific embodiment of the present invention, the mass fraction of ethanol ≥70%.

[0031] In a specific embodiment of the present invention, the mass fraction of ethanol ≥90%.

[0032] In a specific embodiment of the present invention, the extraction temperature is 50 - 80°C.

[0033] In a specific embodiment of the present invention, the extraction time is 1 - 2 h.

[0034] In a specific embodiment of the present invention, the method for preparing the ethanol extract of saussurea involucrata further includes: removing ethanol from the solution obtained after filtration.

[0035] In a specific embodiment of the present invention, reduced - pressure concentration treatment is used to remove ethanol from the solution obtained after filtration.

[0036] In a specific embodiment of the present invention, the temperature of the reduced - pressure concentration treatment is 38 - 55°C.

[0037] In a specific embodiment of the present invention, the temperature of the reduced - pressure concentration treatment is 42 - 48°C.

[0038] In a specific embodiment of the present invention, before the saussurea involucrata is mixed with ethanol, the saussurea involucrata is sterilized.

[0039] In a specific embodiment of the present invention, the sterilization treatment includes high - pressure steam sterilization.

[0040] In a specific embodiment of the present invention, the pressure of the high - pressure steam sterilization is 103.4 - 205.8 kPa.

[0041] In a specific embodiment of the present invention, the temperature of the high - pressure steam sterilization is 120 - 132°C.

[0042] In a specific embodiment of the present invention, a saussurea involucrata extract is further provided, which is prepared by the above - mentioned preparation method.

[0043] In a specific embodiment of the present invention, there is also provided an application of a saussurea involucrata extract in the preparation of cosmetics. The saussurea involucrata extract includes the saussurea involucrata extract prepared by the above preparation method and the above saussurea involucrata extract.

[0044] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0045] By using polyols in combination with an adsorbent to redissolve and purify the alcohol extract of saussurea involucrata, it is possible to effectively remove / fade the unpleasant odor / stink of the obtained saussurea involucrata extract, fade the color of the saussurea involucrata extract, and enhance its application in the cosmetic field;

[0046] The stability of the obtained saussurea involucrata extract is further improved, and the content of the target components therein is high, the loss is small, and the anti-inflammatory, antioxidant and whitening effects are remarkable. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0048] Figure 1 It is the HPLC chromatogram of the redissolution solution of Example 1 of the present invention;

[0049] Figure 2 It is the HPLC chromatogram of the saussurea involucrata extract of Example 1 of the present invention;

[0050] Figure 3 It is the HPLC chromatogram of the redissolution solution of Example 3 of the present invention;

[0051] Figure 4 It is the HPLC chromatogram of the saussurea involucrata extract of Example 3 of the present invention;

[0052] Figure 5 It is the graph of the NO production amount and cytotoxicity of the saussurea involucrata extracts of Examples 1 and 3 of the present invention, the redissolution solution, and the saussurea involucrata extract of Comparative Example 1;

[0053] Figure 6 It is the relationship graph of the melanin production amount of the saussurea involucrata extracts of Examples 1 and 3 of the present invention, the redissolution solution, and the saussurea involucrata extract of Comparative Example 1. DETAILED DESCRIPTION OF THE INVENTION

[0054] The technical solutions of the present invention will be described below in conjunction with the accompanying drawings and specific embodiments. However, those skilled in the art will understand that the following described embodiments are some embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present invention. For those conditions not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0055] In a specific embodiment of the present invention, a method for preparing a saussurea involucrata extract is provided, including: the alcohol extract of saussurea involucrata is redissolved and purified by polyol and adsorbent. After the alcohol extract of saussurea involucrata is redissolved and purified by polyol and adsorbent, the obtained saussurea involucrata extract has no obvious unpleasant odor, light color, better stability, higher contents of total flavonoids and total polyphenols in the product, and can more effectively exert functions such as whitening, antioxidant, and anti-inflammatory.

[0056] When redissolving and purifying, polyol is used, so that the active ingredients in the extract obtained by extracting saussurea involucrata with an alcohol-containing solvent can be fully dissolved in the polyol, facilitating the separation or enrichment of the active ingredients in the alcohol extract of saussurea involucrata.

[0057] The applicant found that the water-soluble components and their contents in the alcohol extract of saussurea involucrata have a great influence on the dark color and strong unpleasant odor of the saussurea involucrata extract. Using polyol combined with an adsorbent to redissolve and purify the alcohol extract of saussurea involucrata can effectively adsorb the water-soluble components in the alcohol extract of saussurea involucrata, thereby lightening the unpleasant odor and color of the obtained saussurea involucrata extract. The obtained saussurea involucrata extract retains the characteristic aroma and has almost no unpleasant odor.

[0058] Moreover, the adsorption treatment is carried out by directly mixing the adsorbent. Compared with before the adsorbent treatment, the loss of the target components in the obtained saussurea involucrata extract is very small, and the content change is extremely small, which is more conducive to the application of the saussurea involucrata extract in cosmetics such as creams, essential oils, toners, gels, serums, facial masks, sunscreens, and lotions. In addition, the above treatment method can also effectively remove polar macromolecules such as odors and pigments in the alcohol extract of saussurea involucrata, and can effectively improve the stability of the obtained saussurea involucrata extract.

[0059] It can be understood that the alcohol extract of saussurea involucrata can be the extract obtained by extracting saussurea involucrata with an alcohol-containing solvent and filtering, or the concentrate of the above extract after removing the alcohol-containing solvent to the extract or crude drug amount, which can be adjusted adaptively according to actual needs. When the saussurea involucrata extract is applied to the cosmetic field or related fields, corresponding treatments can also be carried out according to actual needs, such as sterilization treatment, etc.

[0060] In a specific embodiment of the present invention, the reconstitution and purification treatment include: after the alcohol extract of saussurea involucrata is reconstituted with polyol, it is mixed evenly with the adsorbent and filtered. Among them, for the reconstitution solution obtained after the alcohol extract of saussurea involucrata is reconstituted with polyol, when it is mixed evenly with the adsorbent again, conventional mixing methods such as stirring can be used.

[0061] It can be understood that if the alcohol extract of saussurea involucrata is a concentrate after removing the alcohol-containing solvent, such as in the form of an extract or a concentrate concentrated to the crude drug amount, then the alcohol extract of saussurea involucrata can be mixed and reconstituted with polyol; after complete reconstitution, the obtained reconstitution solution is mixed evenly with the adsorbent, and after sufficient reaction, it is filtered to obtain the saussurea involucrata extract. Among them, before mixing with the adsorbent, the mixed solution obtained after the alcohol extract of saussurea involucrata is reconstituted with polyol can also be filtered to remove insoluble substances to obtain a reconstitution solution, and then mixed with the adsorbent.

[0062] If the alcohol extract of saussurea involucrata is an extract solution that has not removed the alcohol-containing solvent, the alcohol-containing solvent therein can be removed by conventional methods and then mixed with polyol for reconstitution to obtain a reconstitution solution; the above extract solution can also be mixed with polyol first and then the alcohol-containing solvent therein can be removed by conventional methods such as concentration. For example, when the alcohol-containing solvent is an ethanol aqueous solution, the extract solution obtained after saussurea involucrata is extracted with the ethanol aqueous solution is the alcohol extract of saussurea involucrata. The above extract solution is mixed with polyol first, and then the ethanol therein is removed by conventional methods such as concentration. The obtained concentrate is reconstituted with polyol and filtered to obtain a reconstitution solution. The above reconstitution solution is mixed evenly with the adsorbent, and after sufficient reaction, it is filtered to obtain the filtrate as the saussurea involucrata extract.

[0063] In a specific embodiment of the present invention, the reconstitution and purification treatment include: mixing the alcohol extract of saussurea involucrata, polyol and the adsorbent evenly and filtering. It can be understood that if the alcohol extract of saussurea involucrata is a concentrate after removing the alcohol-containing solvent, such as in the form of an extract or a concentrate concentrated to the crude drug amount, then the alcohol extract of saussurea involucrata, polyol and the adsorbent can be directly mixed evenly, and after sufficient reaction, it is filtered to obtain the saussurea involucrata extract.

[0064] If the alcohol extract of saussurea involucrata is an extract solution that has not removed the alcohol-containing solvent, the alcohol-containing solvent therein can be removed by conventional methods such as concentration and then mixed evenly with polyol and the adsorbent. After sufficient reaction, it is filtered to obtain the saussurea involucrata extract; the above extract solution can also be mixed with polyol and the adsorbent first, and then the alcohol-containing solvent therein is removed by conventional methods such as concentration, and after sufficient reaction, it is filtered to obtain the saussurea involucrata extract.

[0065] In a specific embodiment of the present invention, the adsorbent includes a polar adsorbent.

[0066] In a specific embodiment of the present invention, the adsorbent comprises at least one of silica gel, macroporous adsorption resin and neutral alumina. Using silica gel, macroporous adsorption resin or neutral alumina as the adsorbent can further improve the adsorption treatment effect and reduce the loss of target components in the product. Among them, the polar macroporous adsorption resin can be macroporous adsorption resins such as D101, AB-8, HP-100, etc. It can be understood that the adsorbent is usually in powder form, and its particle size can be not specifically limited, and the commercially available conventional particle size can also be used.

[0067] In a specific embodiment of the present invention, the adsorbent is further preferably silica gel.

[0068] In a specific embodiment of the present invention, the dosage of the adsorbent is 0.05%-5% of the total mass of the polyol and the alcohol extract of Saussurea involucrata. Keeping the dosage of the adsorbent within a suitable range can effectively improve the adsorption efficiency and adsorption effect.

[0069] It can be understood that the dosage of the adsorbent can also be 0.05%, 0.1%, 0.3%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc. of the total mass of the polyol and the alcohol extract of Saussurea involucrata, or the dosage between any two of the above values.

[0070] In a specific embodiment of the present invention, the dosage of the adsorbent is 0.5%-5% of the total mass of the polyol and the alcohol extract of Saussurea involucrata.

[0071] In a specific embodiment of the present invention, the dosage of the adsorbent is 1%-3% of the total mass of the polyol and the alcohol extract of Saussurea involucrata.

[0072] In a specific embodiment of the present invention, the temperature of the purification treatment is 20-85°C. It can be understood that when the alcohol extract of Saussurea involucrata, polyol and adsorbent are mixed for re-dissolution and purification treatment, the temperature of the purification treatment is the same as that of the re-dissolution. When the alcohol extract of Saussurea involucrata is first re-dissolved with polyol and then mixed with the adsorbent for purification treatment, the temperature of the purification treatment can be the same as or different from that of the re-dissolution.

[0073] Among them, the temperature of the purification treatment can be 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85°C, or the temperature between any two of the above values.

[0074] In a specific embodiment of the present invention, the temperature for the purification treatment is 20 - 50 °C. When the alcohol extract of Saussurea involucrata is first redissolved with a polyol and then mixed with an adsorbent for purification treatment, the temperature for the purification treatment is preferably 20 - 50 °C. Conducting the purification treatment at a relatively low temperature can not only enable the effective interaction between the adsorbent and the redissolved solution but also save costs.

[0075] In a specific embodiment of the present invention, the temperature for the purification treatment is 20 - 30 °C.

[0076] In a specific embodiment of the present invention, the time for the purification treatment is 5 - 90 min. It can be understood that when the alcohol extract of Saussurea involucrata, the polyol, and the adsorbent are mixed for redissolution and purification treatment, the time for the purification treatment is the same as the time for redissolution. When the alcohol extract of Saussurea involucrata is first redissolved with a polyol and then mixed with an adsorbent for purification treatment, the time for the purification treatment may or may not be the same as the time for redissolution.

[0077] Among them, the time for the purification treatment can be 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90 min, or the time between any two of the above values.

[0078] In a specific embodiment of the present invention, the time for the purification treatment is 5 - 30 min.

[0079] In a specific embodiment of the present invention, the time for the purification treatment is 10 - 20 min.

[0080] In a specific embodiment of the present invention, the polyol includes at least one of 1,3 - butanediol and dipropylene glycol.

[0081] In a specific embodiment of the present invention, the mass fraction of the polyol ≥ 50%. It can be understood that the polyol can be a polyol solution with a mass fraction of 100%, or an aqueous polyol solution; when the polyol is an aqueous polyol solution, the mass fraction of the aqueous polyol solution ≥ 50%.

[0082] Using a polyol with a relatively high mass fraction can more effectively improve the redissolution efficiency of the alcohol extract of Saussurea involucrata, further reduce the loss of target components, and can achieve the purpose of removing / fading the unpleasant odor and fading the color of the Saussurea involucrata extract, which is beneficial to forming a stable system.

[0083] In a specific embodiment of the present invention, the mass fraction of the polyol is 50%-65%. When the polyol is an aqueous polyol solution, within this range of the mass fraction of the polyol, it is more conducive to the redissolution of the ethanol extract of Saussurea involucrata, obtaining a more stable redissolved solution, and simultaneously achieving the purpose of removing / fading unpleasant odors and fading the color of the product. The mass fraction of the polyol can be further preferably 50%-60%.

[0084] In a specific embodiment of the present invention, the mass fraction of the polyol is 100%. When a polyol solution with a mass fraction of 100% is used for the polyol, the ethanol extract of Saussurea involucrata can be more fully redissolved in the polyol, which can reduce the loss of target components and obtain a more stable redissolved solution; moreover, when redissolving the ethanol extract of Saussurea involucrata with a 100% mass fraction of polyol, it can also effectively remove / fade the unpleasant odor of the obtained Saussurea involucrata extract and fade the color of the Saussurea involucrata extract.

[0085] In a specific embodiment of the present invention, during redissolution and purification, the mass ratio of the ethanol extract of Saussurea involucrata to the polyol is 1:2 - 1:50. It can be understood that the mass ratio of the ethanol extract of Saussurea involucrata to the polyol can be specifically adjusted according to actual needs, and the preferred range can be selected within the above range.

[0086] It can be understood that if the ethanol extract of Saussurea involucrata is a concentrate after removing the alcohol-containing solvent, the mass ratio of the ethanol extract of Saussurea involucrata to the polyol is preferably within the above range. If the ethanol extract of Saussurea involucrata is an extract solution that has not yet removed the alcohol-containing solvent, the alcohol-containing solvent can be removed by a conventional treatment method and then mixed with the polyol. At this time, the mass ratio between the concentrate after removing the alcohol-containing solvent and the polyol is preferably maintained within the above range.

[0087] In a specific embodiment of the present invention, during redissolution and purification, the mass ratio of the ethanol extract of Saussurea involucrata to the polyol is 1:4 - 1:30.

[0088] In a specific embodiment of the present invention, the redissolution temperature is 20 - 85°C. Similarly, it can be understood that when the ethanol extract of Saussurea involucrata, the polyol, and the adsorbent are mixed for redissolution and purification, the redissolution temperature is the same as the purification temperature.

[0089] When the ethanol extract of Saussurea involucrata is first redissolved with the polyol and then mixed with the adsorbent for purification, the redissolution temperature and the purification temperature can be the same or different; in addition, in this treatment method, the redissolution time and the purification time can also be the same or different. The redissolution time can be adjusted adaptively according to the redissolution situation. It is sufficient when the ethanol extract of Saussurea involucrata is fully redissolved, and usually, it can be treated for 0.5 - 2 h.

[0090] Among them, the temperature for reconstitution can be 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85 °C, or the temperature between any two of the above values.

[0091] In a specific embodiment of the present invention, the temperature for reconstitution is 40 - 80 °C. Using a relatively high temperature for reconstitution is beneficial to improving the reconstitution efficiency. It can be understood that when the ethanol extract of Saussurea involucrata is first reconstituted with polyol and then mixed with an adsorbent for purification, the temperature for reconstitution is preferably 40 - 80 °C; the temperature for purification is preferably 20 - 50 °C. When the ethanol extract of Saussurea involucrata is first reconstituted with polyol and then mixed with an adsorbent for purification, the time for reconstitution is preferably 40 - 90 min; the time for purification is preferably 5 - 30 min.

[0092] In a specific embodiment of the present invention, the ethanol extract of Saussurea involucrata can be prepared or can be a commercially available product.

[0093] In a specific embodiment of the present invention, the preparation method of the ethanol extract of Saussurea involucrata includes: Saussurea involucrata is extracted with ethanol, and the mass - volume ratio of Saussurea involucrata to ethanol is 1:10 - 1:40; it is extracted at 45 - 85 °C for 1 - 4 h and then filtered.

[0094] Saussurea involucrata can be a plant of the genus Saussurea DC. in the family Asteraceae. Among them, the Saussurea involucrata used for preparing the ethanol extract of Saussurea involucrata can be the whole plant of Saussurea involucrata; it can also be the above - ground part of Saussurea involucrata, such as the flower part of Saussurea involucrata, or the leaf part of Saussurea involucrata, or the flower part and leaf part of Saussurea involucrata; it can also be the underground part of Saussurea involucrata, such as the root of Saussurea involucrata, etc.; preferably, the whole plant of Saussurea involucrata is used. Saussurea It can be understood that when extracting Saussurea involucrata with ethanol, the number of extraction times can be one or more, and can be adjusted adaptively according to the actual extraction situation. Among them, the extraction time of 1 - 4 h at 45 - 85 °C is the time range for single - time extraction. When multiple extractions are carried out, the time for any single extraction can be respectively within the range of 1 - 4 h.

[0095]

[0096] For example, when two extractions are carried out, Saussurea involucrata can be mixed with a certain amount of ethanol, extracted at a certain temperature for 3 h, and filtered to obtain the first filter residue and the first filtrate; the above - mentioned first filter residue is then mixed with a certain amount of ethanol, extracted at a certain temperature for 3 h, and after filtration, the second filtrate is obtained. The above - mentioned first filtrate and the second filtrate are combined to obtain the ethanol extract of Saussurea involucrata. It should be noted that when multiple extractions are carried out, the conditions for each extraction (such as the material - liquid ratio, temperature, extraction time, etc.) can be the same or different.

[0097] In a specific embodiment of the present invention, the mass-volume ratio of saussurea involucrata to ethanol is 1:15 - 1:30.

[0098] In a specific embodiment of the present invention, the mass-volume ratio of saussurea involucrata to ethanol is 1:18 - 1:25.

[0099] In a specific embodiment of the present invention, the mass fraction of ethanol ≥ 70%. Ethanol can be an ethanol solution with a mass fraction of 100%, or an ethanol aqueous solution. When ethanol is an ethanol aqueous solution, the mass fraction of the ethanol aqueous solution ≥ 70%. Using ethanol with a relatively high mass fraction as the extraction solvent can effectively reduce the leaching of water-soluble substances in saussurea involucrata, improve the leaching of target components, and is beneficial to lightening the unpleasant odor and color of the obtained saussurea involucrata extract. Among them, before extracting saussurea involucrata with ethanol, ethanol can also be used to soak saussurea involucrata for a certain time, and the soaking time can be appropriately selected according to requirements.

[0100] In a specific embodiment of the present invention, the mass fraction of ethanol ≥ 90%.

[0101] In a specific embodiment of the present invention, the extraction temperature can preferably be 50 - 80°C.

[0102] In a specific embodiment of the present invention, the extraction time can preferably be 1 - 2 h.

[0103] In a specific embodiment of the present invention, the preparation method of the ethanol extract of saussurea involucrata further includes: after saussurea involucrata is extracted with ethanol, removing the ethanol in the filtered solution.

[0104] In a specific embodiment of the present invention, reduced pressure concentration treatment is used to remove the ethanol in the filtered solution.

[0105] In a specific embodiment of the present invention, the temperature of the reduced pressure concentration treatment is 38 - 55°C. Performing the reduced pressure concentration treatment at a lower temperature can avoid deepening the color of the obtained product while removing the alcohol-containing solvent ethanol, which is beneficial to further lightening the color of the saussurea involucrata extract. Among them, the solution obtained after ethanol extraction and filtration can be concentrated to an extract state or concentrated to a mass approximately equal to the crude drug amount.

[0106] In a specific embodiment of the present invention, the temperature of the reduced pressure concentration treatment is 42 - 48°C.

[0107] In a specific embodiment of the present invention, before mixing saussurea involucrata with ethanol, saussurea involucrata can be pulverized to improve the extraction efficiency.

[0108] In a specific embodiment of the present invention, before mixing saussurea involucrata with ethanol, sterilization treatment can also be performed.

[0109] In a specific embodiment of the present invention, the sterilization treatment includes autoclaving. Before extracting snow lotus with ethanol, autoclaving the snow lotus can not only effectively solve the problem of microbial residue, but also destroy the tissue structure of the snow lotus raw material and improve the dissolution efficiency of active ingredients in the snow lotus.

[0110] In a specific embodiment of the present invention, the pressure of autoclaving is 103.4 - 205.8 kPa.

[0111] In a specific embodiment of the present invention, the temperature of autoclaving is 120 - 132 °C. It can be understood that the time of autoclaving can be adjusted adaptively according to the actual situation, for example, it can be 10 - 50 min.

[0112] In a specific embodiment of the present invention, a snow lotus extract is also provided, which is prepared by the above method.

[0113] In a specific embodiment of the present invention, the mass ratio of total polyphenols to total flavonoids in the snow lotus extract is (0.5 - 1.2):(1.5 - 6); the above ratio is further preferably (0.7 - 1):(2 - 5), and the above ratio is further preferably (0.79 - 0.95):(3.2 - 4). Keeping the mass ratio of total polyphenols to total flavonoids in the snow lotus extract within the above range enables it to have good anti-inflammatory, whitening and antioxidant effects.

[0114] In a specific embodiment of the present invention, the snow lotus extract includes chlorogenic acid, rutin, 1,5 - dicaffeoylquinic acid and arctiin.

[0115] In a specific embodiment of the present invention, an application of the snow lotus extract in preparing cosmetics is also provided, and the snow lotus extract is the above snow lotus extract or the snow lotus extract prepared by the above preparation method.

[0116] The following is further illustrated by specific examples and experimental examples.

[0117] Example 1

[0118] A preparation method of snow lotus extract, comprising:

[0119] 1) Add 100 g of dry whole - plant raw material of snow lotus, without sieving the coarse powder, and then soak it in 2000 mL of 95% ethanol for 0.5 h; then heat it to 75 °C and extract for 2 h, filter to obtain solution A and filter residue;

[0120] 2) The filter residue is added again to 2000 mL of 95% ethanol and extracted at 75 °C for 2 h, then filtered to obtain Solution B. Solutions A and B are combined and concentrated under reduced pressure by rotary evaporation at 45 °C to the crude drug amount to obtain the ethanol extract of Saussurea involucrata;

[0121] 3) The ethanol extract of Saussurea involucrata is mixed with 100% 1,3-butanediol in a mass ratio of 1:4, stirred and redissolved at 70 °C for 1 h, cooled to room temperature, and filtered to obtain the redissolved solution;

[0122] 4) The redissolved solution is mixed with silica gel powder, stirred at room temperature for 15 min, filtered, and sterilized to obtain the Saussurea involucrata extract; wherein, the dosage of silica gel powder is 0.05% of the mass of the redissolved solution.

[0123] Example 2

[0124] A method for preparing the Saussurea involucrata extract, comprising:

[0125] 1) 100 g of dry whole plant raw material of Saussurea involucrata, the coarse powder is not sieved, and sterilized at 103.4 Kpa and 121.3 °C for 20 min;

[0126] 2) Then add it to 2000 mL of 95% ethanol and soak for 0.5 h; then heat up to 75 °C and extract for 2 h, filter to obtain Solution A and the filter residue;

[0127] 3) The filter residue is added again to 2000 mL of 95% ethanol and extracted at 75 °C for 2 h, filtered to obtain Solution B. Solutions A and B are combined and concentrated under reduced pressure by rotary evaporation at 48 °C to the crude drug amount to obtain the ethanol extract of Saussurea involucrata;

[0128] 4) The ethanol extract of Saussurea involucrata is mixed with 100% dipropylene glycol in a mass ratio of 1:30, stirred and redissolved at 70 °C for 1 h, cooled to room temperature, and filtered to obtain the redissolved solution;

[0129] 5) The redissolved solution is mixed with silica gel powder, stirred at 40 °C for 10 min, filtered, and sterilized to obtain the Saussurea involucrata extract; wherein the dosage of silica gel powder is 0.5% of the mass of the redissolved solution.

[0130] Example 3

[0131] A method for preparing the Saussurea involucrata extract, comprising:

[0132] 1) 100 g of dry whole plant raw material of Saussurea involucrata, the coarse powder is not sieved, then add it to 2000 mL of 70% ethanol and soak for 0.5 h; then heat up to 75 °C and extract for 1 h, filter to obtain Solution A and the filter residue;

[0133] 2) The filter residue is added again to 2000 mL of 70% ethanol and extracted at 75 °C for 1 h, then filtered to obtain solution B. Solutions A and B are combined and concentrated under reduced pressure by rotary evaporation at 55 °C until the crude drug amount is reached, obtaining the ethanol extract of Saussurea involucrata.

[0134] 3) The ethanol extract of Saussurea involucrata is mixed with 80% 1,3-butanediol in a mass ratio of 1:4, stirred and redissolved at 85 °C for 30 min, cooled to room temperature, and filtered to obtain the redissolved solution.

[0135] 4) The redissolved solution is mixed with silica gel powder, stirred at 50 °C for 5 min, filtered, and sterilized to obtain the Saussurea involucrata extract; among them, the dosage of silica gel powder is 3% of the mass of the redissolved solution.

[0136] Example 4

[0137] A method for preparing the Saussurea involucrata extract, comprising:

[0138] 1) 100 g of dry whole plant raw material of Saussurea involucrata, the coarse powder is not sieved, sterilized at 205.8 KPa and 132 °C for 10 min;

[0139] 2) Then it is added to 2000 mL of 70% ethanol and soaked for 0.5 h; then the temperature is raised to 50 °C and extracted for 1 h, filtered to obtain solution A and the filter residue;

[0140] 3) The filter residue is added again to 2000 mL of 70% ethanol, extracted at 50 °C for 1 h, filtered to obtain solution B. Solutions A and B are combined and concentrated under reduced pressure by rotary evaporation at 45 °C until the crude drug amount is reached, obtaining the ethanol extract of Saussurea involucrata;

[0141] 4) The ethanol extract of Saussurea involucrata is mixed with 50% 1,3-butanediol in a mass ratio of 1:4, stirred and redissolved at 70 °C for 30 min, cooled to room temperature, and filtered to obtain the redissolved solution;

[0142] 5) The redissolved solution is mixed with silica gel powder, stirred at 20 °C for 30 min, filtered, and sterilized to obtain the Saussurea involucrata extract; among them, the dosage of silica gel powder is 3% of the mass of the redissolved solution.

[0143] Example 5

[0144] A method for preparing the Saussurea involucrata extract, comprising:

[0145] 1) 100 g of dry whole plant raw material of Saussurea involucrata, the coarse powder is not sieved, sterilized at 121.3 Kpa and 121.3 °C for 20 min;

[0146] 2) Then it is added to 1500 mL of 70% ethanol and soaked for 0.5 h; then the temperature is raised to 50 °C and extracted for 2 h, filtered to obtain solution A and the filter residue;

[0147] 3) The filter residue is added again to 1500 mL of 70% ethanol, extracted at 50 °C for 2 h, filtered to obtain Solution B. Solutions A and B are combined and concentrated under reduced pressure by rotary evaporation at 55 °C to the crude drug amount to obtain the ethanol extract of Saussurea involucrata.

[0148] 4) The ethanol extract of Saussurea involucrata and 60% 1,3-butanediol are mixed at a mass ratio of 1:2, stirred and redissolved at 85 °C for 30 min, cooled to room temperature, and filtered to obtain the redissolved solution.

[0149] 5) The redissolved solution is mixed with D101 macroporous adsorption resin, stirred at 50 °C for 15 min, filtered, and sterilized to obtain the Saussurea involucrata extract; wherein, the dosage of D101 macroporous adsorption resin is 5% of the mass of the redissolved solution.

[0150] Example 6

[0151] A preparation method of Saussurea involucrata extract, comprising:

[0152] 1) 100 g of dry whole plant raw material of Saussurea involucrata is coarsely powdered without sieving, and then added to 3000 mL of 95% ethanol and soaked for 0.5 h; then the temperature is raised to 75 °C for extraction for 2 h, filtered to obtain Solution A and the filter residue;

[0153] 2) The filter residue is added again to 3000 mL of 95% ethanol and extracted at 75 °C for 2 h, filtered to obtain Solution B. Solutions A and B are combined and concentrated under reduced pressure by rotary evaporation at 45 °C to the crude drug amount to obtain the ethanol extract of Saussurea involucrata.

[0154] 3) The ethanol extract of Saussurea involucrata, 100% 1,3-butanediol and silica gel powder are mixed, stirred and redissolved and purified at 70 °C for 1 h, cooled to room temperature, filtered, and sterilized to obtain the Saussurea involucrata extract; wherein, the mass ratio of the ethanol extract of Saussurea involucrata to 1,3-butanediol is 1:4, and the dosage of silica gel powder is 2% of the total mass of the ethanol extract of Saussurea involucrata and 1,3-butanediol.

[0155] Example 7

[0156] A preparation method of Saussurea involucrata extract, comprising:

[0157] 1) The ethanol extract of Saussurea involucrata in the form of an extract (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd.) commercially available and 100% 1,3-butanediol are mixed at a mass ratio of 1:4, stirred and redissolved at 80 °C for 1 h, cooled to room temperature, and filtered to obtain the redissolved solution.

[0158] 2) The redissolved solution is mixed with silica gel powder, stirred at 25 °C for 20 min, filtered, and sterilized to obtain the Saussurea involucrata extract; wherein, the dosage of silica gel powder is 3% of the mass of the redissolved solution.

[0159] Example 8

[0160] Preparation method of saussurea involucrata extract, comprising:

[0161] 1) Add 100 g of dry whole plant raw materials of saussurea involucrata, without sieving the coarse powder, then soak it in 2000 mL of 95% ethanol for 0.5 h; then heat it to 75 °C and extract for 2 h, filter to obtain solution A; solution A is concentrated by rotary evaporation under reduced pressure at 45 °C to the crude drug amount to obtain the alcohol extract of saussurea involucrata;

[0162] 2) Mix the alcohol extract of saussurea involucrata with 100% 1,3-butanediol according to a mass ratio of 1:4, stir and redissolve at 70 °C for 1 h, cool to room temperature, filter to obtain the redissolved solution;

[0163] 3) Mix the redissolved solution with silica gel powder, stir at room temperature for 15 min, filter, sterilize to obtain the saussurea involucrata extract; wherein, the dosage of silica gel powder is 1% of the mass of the redissolved solution.

[0164] Comparative example 1

[0165] Preparation method of saussurea involucrata extract, comprising:

[0166] 1) Add 100 g of dry whole plant raw materials of saussurea involucrata, without sieving the coarse powder, then add it to 2000 mL of 70% ethanol, heat to 80 °C and extract for 2 h, filter to obtain solution A and filter residue;

[0167] 2) Add the filter residue to 2000 mL of 50% ethanol again, extract at 85 °C for 2 h, filter to obtain solution B, combine solution A and B, concentrate by rotary evaporation under reduced pressure at 70 °C to the crude drug amount to obtain the alcohol extract of saussurea involucrata;

[0168] 3) Mix the alcohol extract of saussurea involucrata with 50% 1,3-butanediol according to a mass ratio of 1:4, stir and redissolve at 50 °C for 60 min, cool to room temperature, filter, sterilize to obtain the saussurea involucrata extract.

[0169] Comparative example 2

[0170] Preparation method of saussurea involucrata extract, comprising:

[0171] 1) Add 100 g of dry whole plant raw materials of saussurea involucrata, without sieving the coarse powder, then soak it in 2000 mL of 95% ethanol for 0.5 h; then heat it to 75 °C and extract for 2 h, filter to obtain solution A and filter residue;

[0172] 2) Add the filter residue to 2000 mL of 95% ethanol again, extract at 75 °C for 2 h, filter to obtain solution B, combine solution A and B, concentrate by rotary evaporation under reduced pressure at 45 °C to the crude drug amount to obtain the alcohol extract of saussurea involucrata;

[0173] 3) Mix the alcohol extract of Saussurea involucrata with 100% 1,3-butanediol at a mass ratio of 1:4, stir and redissolve at 70 °C for 1 h, cool to room temperature, and filter to obtain a redissolved solution;

[0174] 4) Mix the redissolved solution with β-cyclodextrin, stir at room temperature for 15 min, filter, and sterilize to obtain the Saussurea involucrata extract; wherein, the dosage of β-cyclodextrin is 1% of the redissolved solution.

[0175] Comparative Example 3

[0176] A method for preparing the Saussurea involucrata extract, comprising:

[0177] 1) Take 100 g of dry whole plant raw material of Saussurea involucrata, without sieving the coarse powder, sterilize at 205.8 Kpa and 132 °C for 10 min;

[0178] 2) Then add it to 1500 mL of 70% ethanol and soak for 0.5 h, then raise the temperature to 45 °C and extract for 1 h, filter to obtain solution A and filter residue;

[0179] 3) Add the filter residue to 1500 mL of 70% ethanol again and extract at 75 °C for 1 h, filter to obtain solution B, combine solution A and B, and concentrate and rotary evaporate under reduced pressure at 55 °C to the crude drug amount to obtain the alcohol extract of Saussurea involucrata;

[0180] 4) Mix the alcohol extract of Saussurea involucrata with 80% 1,3-butanediol at a mass ratio of 1:4, stir and redissolve at 85 °C for 30 min, cool to room temperature, and filter to obtain a redissolved solution;

[0181] 5) Mix the redissolved solution with activated clay, stir at 50 °C for 5 min, filter, and sterilize to obtain the Saussurea involucrata extract; wherein, the dosage of activated clay is 5% of the mass of the redissolved solution.

[0182] Comparative Example 4

[0183] A method for preparing the Saussurea involucrata extract, comprising:

[0184] 1) Take 100 g of dry whole plant raw material of Saussurea involucrata, without sieving the coarse powder, sterilize at 121.3 Kpa and 121.3 °C for 20 min;

[0185] 2) Then add it to 2000 mL of 70% ethanol and soak for 0.5 h; then raise the temperature to 50 °C and extract for 2 h, filter to obtain solution A and filter residue;

[0186] 3) Add the filter residue to 2000 mL of 70% ethanol again, extract at 50 °C for 2 h, filter to obtain solution B, combine solution A and B, and concentrate and rotary evaporate under reduced pressure at 55 °C to the crude drug amount to obtain the alcohol extract of Saussurea involucrata;

[0187] 4) Mix the ethanol extract of Saussurea involucrata with water at a mass ratio of 1:2, stir and redissolve at 70 °C for 30 min, cool to room temperature, and filter to obtain a redissolved solution;

[0188] 5) Mix the redissolved solution with D101 macroporous adsorption resin, stir at 50 °C for 15 min, and filter to obtain solution C; wherein, the dosage of D101 macroporous adsorption resin is 5% of the mass of the redissolved solution;

[0189] 6) Mix solution C with 60% 1,3 - butanediol at a mass ratio of 1:2, sterilize to obtain the Saussurea involucrata extract.

[0190] Using the method of this comparative example to prepare the Saussurea involucrata extract, the precipitation phenomenon is obvious.

[0191] Experimental Example 1 Product Odor Evaluation

[0192] Find 21 volunteers to evaluate the odor of the products prepared in Examples 1 - 8 and Comparative Examples 1 - 4 respectively. Score the products from the characteristic odor of the product, the presence or absence of unpleasant odor / stink, the acceptance degree of the product odor, and the degree of liking for the odor. Each index is set with a full score of 10 points, and the average value is taken for comparison. The evaluation results of the usage effects of the examples are shown in Table 1.

[0193] Table 1 Odor Evaluation Table of the Products Obtained in Examples 1 - 8 and Comparative Examples 1 - 4

[0194] Category Characteristic odor Degree of stench Acceptance level Degree of preference Example 1 Saussurea extract 10 0 10 9 Example 2 Saussurea extract 10 1 10 9 Example 3 Saussurea extract 8 3 8 8 Example 3 reconstitution solution 9 7 5 5 Example 4 Saussurea extract 9 2 9 9 Example 5 Saussurea extract 7 4 8 8 Example 6 Saussurea extract 10 0 10 9 Example 7 Saussurea extract 9 2 9 8 Example 8 Saussurea extract 8 3 8 7 Control Example 1 Saussurea extract 3 8 2 2 Control Example 2 Saussurea extract 4 7 6 4 Control Example 3 Saussurea extract 3 8 3 2 Control Example 4 Saussurea extract 2 9 2 1

[0195] As can be seen from the data in Table 1, among Examples 1 - 5, the Saussurea involucrata extract prepared by the method of Example 1 has the highest evaluation, that is, it highly retains the characteristic odor of Saussurea involucrata flowers, has no stink, and is widely liked by volunteers. For the products without silica gel treatment (the redissolved solution of Example 3 and the Saussurea involucrata extract prepared by the method of Comparative Example 1), the stinking substances were found by most of the subjects. Therefore, after silica gel solid - phase extraction, the stink of the Saussurea involucrata extract can be significantly reduced, and the quality of the Saussurea involucrata extract can be improved.

[0196] For the Saussurea involucrata extracts obtained in Comparative Examples 1 - 4, the unpleasant odor of the product cannot be effectively reduced, and at the same time, the loss of target components is relatively large.

[0197] Experimental Example 2

[0198] Detect the total polyphenol and total flavonoid contents of the samples obtained in Examples 1 - 5 and Comparative Examples 1 - 4. The detection method is as follows.

[0199] Detection of total polyphenol content:

[0200] Add 100 μL of each prepared gallic acid standard solution and each sample respectively, and then add 900 μL of deionized water to make up to 1 mL;

[0201] Then add 100 μL of Folin-Ciocalteu reagent respectively and react fully for 3 min;

[0202] Add 200 μL of 7% sodium carbonate solution respectively, and then add 700 μL of deionized water to make up to 2 mL;

[0203] React for 1 h, pipette 200 μL of the reaction solution into a 96-well plate, and measure the absorbance at 700 nm with a microplate reader;

[0204] Calculate the total polyphenol content in the sample through the prepared gallic acid standard curve and the absorbance of the sample.

[0205] Detection of total flavonoid content:

[0206] Add 100 μL of each prepared rutin standard solution and each sample respectively, and then add 300 μL of 95% ethanol;

[0207] Then add 20 μL of 10% Al(NO3)3 solution respectively and react fully for 3 min (Blank: replace with deionized water);

[0208] Add 20 μL of 1 mol / L sodium acetate solution respectively, and then add 560 μL of deionized water to make up to 1 mL;

[0209] React for 30 min, pipette 200 μL of the reaction solution into a 96-well plate, and measure the absorbance at 415 nm with a microplate reader;

[0210] Calculate the total flavonoid content in the sample through the rutin standard curve and the absorbance of the sample.

[0211] The total polyphenol and total flavonoid contents of the Saussurea involucrata extracts obtained in Examples 1-5, Comparative Examples 1-4, and the reconstituted solutions without silica gel treatment in Examples 1 and 3 are shown in Table 2.

[0212] Table 2 Total polyphenol and total flavonoid contents in the products obtained in Examples 1-5 and Comparative Examples 1-4

[0213] Category Total polyphenol content (mg / mL) Total flavonoid content (mg / mL) Example 1 reconstitution solution 0.95 4.0 Example 1 Saussurea extract 0.93 3.8 Example 2 Saussurea extract 0.89 3.6 Example 3 reconstitution solution 0.91 4.0 Example 3 Saussurea extract 0.86 3.2 Example 4 Saussurea extract 0.82 3.7 Example 5 Saussurea extract 0.79 3.5 Control Example 1 Saussurea extract 0.92 4.2 Control Example 2 Saussurea extract 0.42 1.6 Control Example 3 Saussurea extract 0.35 1.2 Control Example 4 Saussurea extract 0.46 1.7

[0214] As can be seen from the data in Table 2, the contents of total polyphenols and flavonoids in the snow lotus extracts prepared in Examples 1-5 are relatively high, indicating that the snow lotus extracts prepared by this method can enrich polyphenols and flavonoid chemical components well, have strong antioxidant activity, and the phenolic hydroxyl groups contained can have a strong scavenging effect on free radicals through electron transfer or hydrogen donation.

[0215] Compared with the products without silica gel treatment (reconstituted solutions), although the contents of total polyphenols and flavonoids in the snow lotus extracts obtained after silica gel treatment in Examples 1 and 3 decreased slightly, the degree of decrease was very small. However, the contents of total polyphenols and flavonoids in the snow lotus extracts obtained after treatment with other reagents such as β-cyclodextrin (Comparative Example 2) and activated clay (Comparative Example 3) decreased significantly, by more than half respectively. Therefore, selecting a specific adsorbent can not only effectively reduce unpleasant odors and colors, but also effectively reduce the loss of target components in the product.

[0216] Experimental Example 3 Stability Observation Data

[0217] The stability observation data of the products obtained in Example 1, Example 3 and Comparative Example 1 are shown in Table 3.

[0218] Table 3 Stability Observation Data of the Products Obtained in Example 1, Example 3 and Comparative Example 1

[0219] Detection index Example 1 reconstitution solution Example 1 Saussurea extract Example 3 reconstitution solution Example 3 Saussurea extract Control Example 1 Saussurea extract Presence or absence of precipitation (day 0) None None None None None Presence or absence of precipitation (room temperature, day 3) None None None None Yes Presence or absence of precipitation (room temperature, day 45) None None Yes None Yes Color (room temperature, day 0) Yellow Light yellow Brownish yellow Yellow Dark brown Color (room temperature, day 45) Dark yellow Light yellow Reddish brown Yellow Dark brown

[0220] There are obvious precipitation phenomena in the reconstituted solution prepared by the method of Example 3 and the snow lotus extract prepared in Comparative Example 1. In addition, it can be found from Table 3 that among them, the stability of the snow lotus extract obtained after silica gel treatment of the reconstituted solution of Example 3 is significantly improved, and it is still a homogeneous and stable system after 45 days of storage; and after silica gel treatment, the color of the snow lotus extract of Example 3 changes from brownish-yellow to yellow, and the color becomes significantly lighter. The color of the snow lotus extract prepared by the preparation method of Example 1 also becomes lighter to a certain extent compared with the reconstituted solution before silica gel treatment, changing from yellow to light yellow; and after 45 days of storage at room temperature, the color of the reconstituted solution before silica gel treatment becomes slightly darker, changing from yellow to dark yellow, while the color of the snow lotus extract obtained after silica gel treatment hardly changes. It can be seen that silica gel adsorption treatment can effectively fade the color of the snow lotus extract and improve the stability of the snow lotus extract; for products with poor stability themselves, after adding silica gel adsorption treatment, the unstable phenomenon of the products can be effectively improved, and the stability and formula applicability of the products can be significantly improved.

[0221] Experimental Example 4 High Performance Liquid Chromatography (HPLC) Substance Composition Analysis

[0222] HPLC Substance Composition Analysis Method:

[0223] The snow lotus extract and the reconstitution solution obtained by the preparation methods of Example 1 and Example 3 were respectively diluted to 10% with methanol. Then, 1.5 mL was sucked with a syringe, filtered through an organic 0.22-μm filter membrane, and injected into a sample bottle for standby as samples.

[0224] For the HPLC chromatogram analysis of the snow lotus extract and the reconstitution solution, an Agilent system was used with an e2695 PAD UV detector. The chromatographic column was Eclipse XBD C18 (5 µm, 4.6 mm × 250 mm). The mobile phases were: A: water (0.1% acetic acid); B: acetonitrile. The injection volume was 5 µL, and the detection wavelength was 280 nm.

[0225] See Figures 1 - 4 , through the HPLC analysis of the composition of the snow lotus extract obtained from Examples 1 and 3 and the reconstitution solution of the product without silica gel treatment, it was found that the snow lotus extract prepared by this method mainly includes bioactive components such as chlorogenic acid, rutin, 1,5-dicaffeoylquinic acid, and arctiin, which are the material basis for the antioxidant, anti-inflammatory, and whitening effects of the snow lotus extract.

[0226] From the HPLC detection data, the relative content ratios of chlorogenic acid, rutin, 1,5-dicaffeoylquinic acid, and arctiin in the product can be obtained. See Table 4 for details. As can be seen from Table 4, the content of chlorogenic acid in the snow lotus extract prepared by this method is the most abundant. Before and after silica gel treatment, the relative content of the target components changes very little, and hardly affects the biological activity of the product.

[0227] Moreover, compared with the reconstitution solution without silica gel treatment, in the snow lotus extract obtained after silica gel treatment, the relative contents of chlorogenic acid and 1,5-dicaffeoylquinic acid slightly decreased, while the relative contents of rutin and arctiin both slightly increased. It can be seen that the purification treatment of the present invention has a certain effect of selectively enriching the target components.

[0228] Table 4 Composition and proportion of the samples obtained in Examples 1 and 3

[0229]

[0230] Experimental Example 5 Anti-inflammatory activity evaluation

[0231] The anti-inflammatory activity was evaluated by the method of inhibiting NO generation. The specific measurement experimental method is as follows:

[0232] The prepared original solution of saussurea involucrata extract / reconstituted solution was diluted with cell culture medium to prepare material solutions with three concentrations of 0.1%, 0.2% and 0.4% respectively. The cells were mouse macrophage RAW264.7 cells. On the first day, the seeding density in 24-well plates was 2×10^5 cells / well. After 18 h, the old culture medium solution was removed, and 1 mL of DMEM cell culture medium containing 1 μg / mL lipopolysaccharide (LPS) was added to each well. Then, the prepared product solutions were added respectively. After culturing in an incubator for 24 h, 100 μL of the supernatant was aspirated from each well, and then an equal volume of Griess reagent was added to measure the NO content. Then, the absorbance A was measured at a wavelength of 540 nm, and the NO production was calculated using the following formula (1) (NO production %), where A control is the absorbance corresponding to the cell model induced only by LPS.

[0233] NO production (%) = × 100% (1)

[0234] For the NO production data of the saussurea involucrata extracts obtained in Examples 1 and 3, the corresponding reconstituted solutions, the saussurea involucrata extract obtained in Comparative Example 1, and the positive control group, please refer to Figure 5 . Among them, the result data corresponding to the "dexamethasone" label is the result data of the product with 10 μg dexamethasone, the "+" label is the result data corresponding to the cell model induced only by LPS, the "-" label is the result data corresponding to the natural state of the cells without LPS induction, the "saussurea involucrata extract of Example 1" label is the result data corresponding to the saussurea involucrata extract prepared in Example 1, the "reconstituted solution of Example 1" label is the result data corresponding to the reconstituted solution prepared in Example 1, the "saussurea involucrata extract of Example 3" label is the result data corresponding to the saussurea involucrata extract prepared in Example 3, the "reconstituted solution of Example 3" label is the result data corresponding to the reconstituted solution prepared in Example 3, and the "Comparative Example 1" label is the result data corresponding to the saussurea involucrata extract prepared in Comparative Example 1.

[0235] From Figure 5 it can be seen that the NO production inhibitory activity experiments were carried out on the saussurea involucrata extracts obtained in Examples 1 and 3, and the reconstituted solutions without silica gel treatment in Examples 1 and 3, indicating that the saussurea involucrata extracts and the corresponding reconstituted solutions obtained in Examples 1 and 3 all have good anti-inflammatory activities.

[0236] Compared with the reconstituted solutions without silica gel treatment in Examples 1 and 3, the change in the anti-inflammatory activity of the saussurea involucrata extracts obtained in Examples 1 and 3 is very small, and the antioxidant activity effect increases slightly, which is consistent with the change in the (relative) content of total polyphenols, total flavonoids and the corresponding target components in the products of Examples 1 and 3 described above.

[0237] Moreover, at a concentration of 0.1% - 0.4%, the snow lotus extract inhibited the production of NO in a concentration gradient manner, showing strong anti-inflammatory activity. In addition, the antioxidant activities of the snow lotus extracts (reconstituted solutions) obtained in Examples 1 and 3 were superior to those of Comparative Example 1, further indicating that the snow lotus extract prepared by the method of the present invention has a higher content of target components.

[0238] Experimental Example 6 Evaluation of Whitening Activity

[0239] The whitening activity was evaluated by detecting the inhibitory activity of melanin production. The specific experimental method is as follows:

[0240] The prepared stock solution of the snow lotus extract / reconstituted solution was diluted with cell culture medium to prepare material solutions with three concentrations of 0.1%, 0.2%, and 0.4% respectively. The cells were mouse melanoma B16F10 cells. On the first day, the seeding density in 24-well plates was 2×10^4 cells / well. After 18 h, the old culture medium solution was removed, and 1 mL of DMEM cell culture medium containing 1 nM neuropeptide α-melanocyte-stimulating hormone (α-MSH) was added to each well. Then, the prepared product solutions were added respectively. After culturing in an incubator for 24 h, the supernatant was discarded. Then, 1 mL of phosphate buffer solution (PBS) was added to wash the adherent cells. After removing the PBS solution, 200 μL of 1N NaOH solution was added to each well, and the cells were dissolved at 80°C for about 30 min. After completion, 100 μL was taken and added to a 96-well plate. The absorbance was measured with an enzyme-linked immunosorbent assay (ELISA) reader at a wavelength of 405 nm to determine the intracellular melanin secretion amount, where A control is the absorbance corresponding to the cell model induced only by α-MSH.

[0241] Melanin production (%)= ×100% (2)

[0242] For the data of the melanin secretion amounts of the snow lotus extracts obtained in Examples 1 and 3 and the corresponding reconstituted solutions, the snow lotus extract obtained in Comparative Example 1, and the positive control group, please refer to Figure 6, where the "kojic acid" label indicates the result data corresponding to the 300 Um kojic acid product, the "+" label indicates the result data corresponding to the cell model induced only by α-MSH, the "-" label indicates the result data corresponding to the natural state of the cells without α-MSH induction, the "Saussurea involucrata extract of Example 1" indicates the result data corresponding to the Saussurea involucrata extract prepared in Example 1, the "reconstitution solution of Example 1" indicates the result data corresponding to the reconstitution solution prepared in Example 1, the "Saussurea involucrata extract of Example 3" indicates the result data corresponding to the Saussurea involucrata extract prepared in Example 3, the "reconstitution solution of Example 3" indicates the result data corresponding to the reconstitution solution prepared in Example 3, and the "Comparative Example 1" indicates the result data corresponding to the Saussurea involucrata extract prepared in Comparative Example 1.

[0243] From Figure 6 It can be seen that melanogenesis inhibition activity experiments were conducted on the Saussurea involucrata extracts obtained in Examples 1 and 3, and the reconstitution solutions without silica gel treatment in Examples 1 and 3, indicating that the Saussurea involucrata extracts and the corresponding reconstitution solutions obtained in Examples 1 and 3 both have good melanogenesis inhibition activity.

[0244] Compared with the reconstitution solutions without silica gel treatment in Examples 1 and 3, the change in the melanogenesis inhibition activity of the Saussurea involucrata extracts obtained in Examples 1 and 3 is very small, and the melanogenesis inhibition activity effect increases slightly, which is also consistent with the (relative) content changes of total polyphenols, total flavonoids, and the corresponding target components in the products of Examples 1 and 3 described above.

[0245] Moreover, at a concentration of 0.1% - 0.4%, the Saussurea involucrata extract inhibited melanogenesis in a concentration gradient and showed stronger potency compared to the positive control kojic acid, demonstrating strong whitening activity. In addition, the whitening activity of the Saussurea involucrata extracts (reconstitution solutions) obtained in Examples 1 and 3 is superior to that of Comparative Example 1, further indicating that the Saussurea involucrata extract prepared by the method of the present invention has a higher content of target components.

[0246] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing examples, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing examples, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a saussurea involucrata extract, characterized in that, Comprising: The alcohol extract of Saussurea involucrata is redissolved and purified by polyhydric alcohol and adsorbent; The redissolving and purifying treatment includes: after the alcohol extract of Saussurea involucrata is redissolved by polyhydric alcohol, it is mixed evenly with the adsorbent and filtered, and the obtained solution is the Saussurea involucrata extract; The adsorbent includes silica gel or macroporous adsorption resin; the dosage of the adsorbent is 0.05%-5% of the total mass of the polyhydric alcohol and the alcohol extract of Saussurea involucrata; The temperature of the purification treatment is 20-85°C; the time of the purification treatment is 5-90 min; The polyhydric alcohol includes at least one of 1,3-butanediol and dipropylene glycol; The mass fraction of the polyhydric alcohol ≥50%; The temperature of the redissolving is 20-85°C; The preparation method of the alcohol extract of Saussurea involucrata includes: Saussurea involucrata is extracted with ethanol, and the mass-volume ratio of Saussurea involucrata to ethanol is 1:10-1:40; It is extracted at 45-85°C for 1-4 h and filtered; The mass fraction of the ethanol ≥70%.

2. The preparation method according to claim 1, characterized in that The preparation method of the alcohol extract of Saussurea involucrata further includes: removing the ethanol in the solution obtained after filtration by vacuum concentration treatment; The temperature of the vacuum concentration treatment is 38-55°C; The temperature of the vacuum concentration treatment is 42-48°C.

3. The preparation method according to claim 2, characterized in that, Before mixing Saussurea involucrata with ethanol, Saussurea involucrata is subjected to high-pressure steam sterilization treatment; the pressure of the high-pressure steam sterilization is 103.4-205.8 kPa; the temperature of the high-pressure steam sterilization is 120-132°C.

4. A Saussurea involucrata extract prepared by the preparation method according to any one of claims 1-3.

5. An application of a Saussurea involucrata extract in the preparation of cosmetics, wherein the Saussurea involucrata extract includes the Saussurea involucrata extract prepared by the preparation method according to any one of claims 1-3 or the Saussurea involucrata extract according to claim 4.

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